WO2018088562A1 - Culture device for alga - Google Patents
Culture device for alga Download PDFInfo
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- WO2018088562A1 WO2018088562A1 PCT/JP2017/040827 JP2017040827W WO2018088562A1 WO 2018088562 A1 WO2018088562 A1 WO 2018088562A1 JP 2017040827 W JP2017040827 W JP 2017040827W WO 2018088562 A1 WO2018088562 A1 WO 2018088562A1
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- tank body
- culture
- tank
- culture solution
- upper lid
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M1/00—Apparatus for enzymology or microbiology
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- the present invention includes a culture tank in which a culture solution of microalgae is stored, an illumination device that irradiates the tank body with light that promotes photosynthesis of microalgae, and a part of the culture solution in the tank body is extracted by a circulation pump.
- the present invention also relates to an algae culture apparatus provided with a culture solution circulation path for refluxing again into the tank body.
- the upper lid 23 is made of a metal plate such as a stainless steel plate having a high light reflectance (Patent Document 1).
- a large number of translucent pipes 24, 24,... Penetrating the tank body 22 in the horizontal direction are arranged in parallel at regular intervals in the vertical and horizontal directions. It is formed into a cylindrical pipe with a high-strength acrylic resin and the like excellent in heat resistance and heat resistance, and is provided so as to penetrate from the one surface side of the rectangular cylindrical body portion 22a of the tank body 22 to the opposite surface side, The penetrating portion is sealed with a sealing material such as packing.
- the illuminating device 25 which irradiates the tank main body 22 of the culture tank 21 with the light which promotes the photosynthesis of microalgae is inserted in the translucent pipes 24, 24 ... penetrating the tank main body 22 in the horizontal direction.
- a straight tube type fluorescent lamp is used as the light source 26.
- a part of the culture solution W in the tank body 22 is extracted by a circulation pump 29 from a drain port 28 provided in the bottom 22 b of the tank body 22, and is returned to the tank body 22 again.
- a circulation path 30 is provided.
- the culture medium circulation path 30 includes a pipe 30 a that forms the path and a drain pipe 31 that discharges the culture medium W in the tank body 22 to the drain port 28 provided in the bottom 22 a of the tank body 22.
- CO 2 carbon dioxide
- the body 22a of the tank body 22 has a pair of left and right upper culture medium discharge nozzles 36R and 36L that discharge the culture medium refluxed through the culture medium circulation path 30 from the upper side of the body 22a in the horizontal direction.
- a pair of left and right lower culture medium discharge nozzles 37R and 37 for discharging from the lower side of the body 22a in a direction parallel to the bottom 22b is attached to stir and flow the culture medium W in the tank body 22. Yes.
- the algae culture apparatus configured as described above is a translucent pipe in which the light sources 26, 26,... Of the illuminating device 25 that irradiates light into the tank body 22 of the culture tank 21 are provided so as to penetrate the tank body 22. 24, 24... Are inserted into the inside of the culture tank 21 so as to be freely inserted and removed, so that the light source 26 can be exchanged or maintained while the top cover 23 of the culture tank 21 is closed.
- the light sources 26, 26,... Of the illuminating device 25 that irradiates light into the tank body 22 of the culture tank 21 are provided so as to penetrate the tank body 22. 24, 24... are inserted into the inside of the culture tank 21 so as to be freely inserted and removed, so that the light source 26 can be exchanged or maintained while the top cover 23 of the culture tank 21 is closed.
- harmful microorganisms, foreign substances, etc. enter the culture tank 21 and the culture solution W in the tank body 22 is not contaminated.
- the tank main body 22 is reflected light by being omnidirectionally radiated along Since the light irradiation density in the tank body 22 is substantially uniform as a whole because it is made of a high-rate metal plate such as a stainless steel plate, the microalgae in the culture medium W in the tank body 22 Even if the culture concentration is high, it is possible to uniformly irradiate light that promotes photosynthesis of microalgae throughout the tank body 22.
- the culture medium that recirculates into the tank body 22 through the culture medium circulation path 30 is a pair of left and right upper culture liquid discharge nozzles 36R and 36L and a lower culture liquid discharge nozzle that are attached to the body 22a of the tank main body 22. Since the culture liquid W in the tank body 22 is agitated and fluidized by being discharged from 37R and 37 in the horizontal direction, the light from the light source 26 of the illumination device 25 is uniformly irradiated to the microalgae in the culture liquid W. Will be.
- the conventional apparatus shown in FIGS. 2 and 3 having the advantages as described above is capable of culturing a large amount of high-quality microalgae with high efficiency and high concentration using a large-capacity culture tank 21.
- carbon dioxide gas (CO 2 ) essential for photosynthesis of the algae is required in the culture liquid W of microalgae stored in the tank body 22 of the culture tank 21. It is necessary to inject and dissolve the amount.
- CO 2 carbon dioxide
- the culture solution discharge nozzles 36R and 36L attached to the upper side of the body portion 22a are disposed so as to discharge the culture solution refluxed into the tank body 22 in the horizontal direction
- the culture solution discharge nozzles 36R and 36L are disposed in the discharged culture solution. Since the bubbles of CO 2 contained in the liquid instantly float on the surface of the culture solution W and escape from the culture solution, CO 2 in the culture solution W irradiated with light that promotes photosynthesis of microalgae by the lighting device 25 is emitted. It was difficult to cultivate microalgae with high efficiency by increasing the amount of dissolution.
- oxygen gas (O 2 ) generated by photosynthesis of the algae was obtained.
- O 2 oxygen gas
- it accumulates and accumulates in a large amount in the space formed between the liquid level of the culture medium W in the tank body 22 and the upper lid 23 that closes the upper surface of the tank body 22, thereby inhibiting the growth and growth of microalgae. It turned out to be a factor.
- the present invention the microalgae to mass culture with high efficiency, of the culture tank to increase the CO 2 dissolved amount of culture medium which is stored in the tank body, that the microalgae increase its CO 2 dissolved amount
- the amount of oxygen gas (O 2 ) generated by photosynthesis increases, and the oxygen gas stays and accumulates in a large amount in the space formed between the liquid level of the culture solution in the tank body and the upper lid that closes the upper surface of the tank body. Therefore, it is a technical problem to prevent the algal breeding / growth from being inhibited.
- the present invention provides an illumination device that irradiates light that promotes photosynthesis of microalgae into a tank body of a culture tank in which a culture solution of microalgae is stored, and a culture solution in the tank body.
- An algae culture apparatus comprising a culture medium circulation path for extracting carbon dioxide (CO 2 ) into the liquid by injecting the part with a circulation pump and recirculating it into the tank main body.
- a plurality of culture solution discharge nozzles are attached to discharge the culture solution that circulates through the circulation path from the upper side to the bottom side of the body of the tank body, and the upper lid and the inside of the tank body are attached to the upper lid that closes the upper surface of the tank body.
- a ventilator for releasing oxygen gas (O 2 ) staying in a space formed between the liquid level of the culture solution stored in the outside is attached.
- CO 2 carbon dioxide
- the inside of the culture medium in the tank body is once lowered to the bottom side of the tank body and then turned upward from the bottom side to the liquid level of the culture medium in the tank body.
- the residence time of CO 2 in the culture solution becomes longer, the amount of CO 2 dissolved increases, and photosynthesis of microalgae is promoted.
- oxygen gas (O 2 ) generated by photosynthesis of microalgae and staying in the space between the liquid level of the culture medium in the tank body and the upper lid of the tank body is released to the outside by a ventilation device attached to the upper lid. It is released and the oxygen gas (O 2 ) is prevented from inhibiting the growth and growth of microalgae.
- the schematic diagram which shows schematically the algal culture apparatus which concerns on the Example of this invention.
- An algae culture apparatus includes an illumination device that irradiates light that promotes photosynthesis of microalgae into a tank body of a culture tank in which a culture solution of microalgae such as Haematococcus algae is stored; A culture medium circulation path for extracting a part of the culture medium with a circulation pump, replenishing and injecting carbon dioxide (CO 2 ) into the liquid and recirculating it into the tank body.
- an illumination device that irradiates light that promotes photosynthesis of microalgae into a tank body of a culture tank in which a culture solution of microalgae such as Haematococcus algae is stored
- a culture medium circulation path for extracting a part of the culture medium with a circulation pump, replenishing and injecting carbon dioxide (CO 2 ) into the liquid and recirculating it into the tank body.
- CO 2 carbon dioxide
- a plurality of culture solution discharge nozzles are attached to discharge the culture solution that circulates through the path from the upper side to the bottom side of the tank body, and the upper lid that closes the upper surface of the tank body
- a ventilation device is attached to discharge high-concentration oxygen gas (O 2 ) staying in the upper space generated between the culture solution stored in the chamber and the outside.
- the ventilator includes an exhaust port attached to an upper lid that closes an upper surface of a tank body of a culture tank, a ventilation fan that discharges gas in the tank body from the exhaust port, an electric shutter that opens and closes the exhaust port, and the upper lid
- the concentration of oxygen gas (O 2 ) staying in the upper space generated between the liquid and the liquid level of the culture solution stored in the tank body is measured, and the measured value of the O 2 concentration exceeds a certain value.
- the oximeter has an O 2 sensor that is attached to the upper lid of the culture tank and detects the O 2 concentration in the tank body.
- another embodiment of the ventilation device includes an exhaust port of ⁇ 25 to ⁇ 70 mm attached to the upper lid, and a small-diameter ventilation fan that constantly discharges gas in the tank body from the exhaust port.
- the algae culture apparatus according to the embodiment of the present invention shown in FIG. 1 is a stainless steel plate in which a tank main body 2 of a culture tank 1 in which a culture solution W of microalgae is stored and an upper lid 3 that closes the upper surface of the culture tank It is made with.
- the culture tank 1 is equipped with the illuminating device which irradiates the light which accelerates
- the illuminating device may be the same type as the illuminating device of FIGS. 2 and 3 using a straight tube fluorescent lamp as a light source, or may be an LED or organic EL as a light source, and its configuration is not limited. .
- the culture tank 1 has a tank body 2 composed of a rectangular tube-shaped body 2 a and a square funnel-shaped bottom 2 b, and a circulation pump 5 removes a part of the culture solution W stored in the tank body 2.
- a culture solution circulation path 4 is provided which is extracted from the bottom 2b side, replenished and injected into the culture solution with CO 2 replenishment device 6 and recirculated into the tank body 2.
- a pair of left and right culture solution discharge nozzles for discharging the culture solution refluxed through the culture solution circulation path 4 obliquely downward toward the bottom 2b side of the tank body 2 7R, 7L and 8R, 8L are attached.
- the upper lid 3 that closes the upper surface of the tank body 2 has a high-concentration oxygen gas (O) that stays in the space formed between the upper lid 3 and the liquid level of the culture medium W stored in the tank body 2.
- a ventilator 10 for releasing the outside is attached.
- the ventilation device 10 includes an exhaust port 11 attached to the upper lid 3 of the culture tank 1, a ventilation fan 12 that discharges gas in the tank body 2 from the exhaust port 11, and an electric shutter 13 that opens and closes the exhaust port 11.
- the concentration of oxygen gas (O 2 ) staying in the space formed between the upper lid 2 of the culture tank 1 and the liquid level of the culture solution W stored in the tank body 2 is measured, and the O 2 is measured.
- the ventilation fan 12 is started and the electric shutter 13 is opened at the same time.
- the ventilation fan 12 is stopped and the motor is operated at the same time. and comprises an oxygen meter 14 which outputs a control signal for closing the shutter 13, to the oxygen meter 14, O 2 sensor for detecting the O 2 concentration in the tank body is attached to the upper lid of the culture tank 15 is provided.
- oxygen gas (O 2 ) generated by photosynthesis of microalgae and staying in the space between the liquid level of the culture solution in the tank body 2 and the upper lid 3 is caused by the ventilation device 10 attached to the upper lid 3.
- the oxygen gas (O 2 ) released to the outside is prevented from inhibiting the growth and growth of microalgae.
- the ventilator 10 can prevent entry of foreign matter such as dust and insects into the culture tank 1 by opening and closing the exhaust port 11 with the electric shutter 13 in accordance with the start / stop of the ventilation fan 12. It has become a thing.
- the ventilator of the present invention has a ⁇ 25 to ⁇ 70 mm exhaust port attached to the upper lid 3 that closes the upper surface of the tank main body 2 of the culture tank 1, and the gas in the tank main body 2 from the exhaust port.
- a small-diameter ventilation fan that constantly discharges the gas, and if the small-diameter ventilation fan is continuously operated to release the gas in the tank body 2 constantly, the foreign matter from the exhaust port Intrusion can also be prevented.
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Abstract
A culture device for an alga, wherein the amount of CO2 dissolved in a culture liquid, said culture liquid being pooled in a tank body of a culture tank, is increased, and the retention and accumulation of oxygen gas (O2), said oxygen gas being generated by photosynthesis of microalgae, in the inner space of the tank body are blocked to thereby prevent the inhibition of the proliferation or growth of the alga. The culture device is provided with: culture liquid-discharging nozzles 7R and 7L for discharging the culture liquid, which contains CO2 blown thereinto and which is refluxed through a culture liquid circulation path 4, from the top side of the barrel of the tank body 2 toward the bottom side thereof; and a ventilator 10 for releasing the high-concentration oxygen gas (O2) outside, said oxygen gas (O2) being generated by photosynthesis of the microalgae and retained in the inner space of the tank body 2.
Description
本発明は、微細藻類の培養液が貯留される培養タンクと、そのタンク本体内に微細藻類の光合成を促す光を照射する照明装置と、タンク本体内の培養液の一部を循環ポンプで抜き出して再びタンク本体内に還流させる培養液循環径路とを備えた藻類培養装置に関する。
The present invention includes a culture tank in which a culture solution of microalgae is stored, an illumination device that irradiates the tank body with light that promotes photosynthesis of microalgae, and a part of the culture solution in the tank body is extracted by a circulation pump. The present invention also relates to an algae culture apparatus provided with a culture solution circulation path for refluxing again into the tank body.
この種の藻類培養装置としては、図２に正面視縦断面図を示し、図３に側面視縦断面図を示す装置が提案されており、当該装置は、ヘマトコッカス藻（Haematococcus pluvialis）などの微細藻類を培養する培養液Ｗが貯留される培養タンク２１のタンク本体２２が、角筒状の胴部２２ａと角型漏斗状の底部２２ｂとからなり、当該タンク本体２２と、その上面を密閉する上蓋２３とが、光反射率の高いステンレス鋼板等の金属板で製作されている（特許文献１）。
As an algae culture apparatus of this kind, an apparatus having a longitudinal sectional view in front view in FIG. 2 and a longitudinal sectional view in side view in FIG. 3 has been proposed. A tank body 22 of a culture tank 21 in which a culture solution W for cultivating microalgae is composed of a cylindrical body 22a and a square funnel-shaped bottom 22b, and the tank body 22 and its upper surface are sealed. The upper lid 23 is made of a metal plate such as a stainless steel plate having a high light reflectance (Patent Document 1).
培養タンク２１には、そのタンク本体２２を水平方向に貫通する多数本の透光性パイプ２４、２４…が上下左右方向に一定間隔で並設され、それら各透光性パイプ２４は、光透過性と耐熱性に優れた高強度のアクリル系樹脂等で円筒形パイプに成形されて、タンク本体２２の角筒状胴部２２ａの一面側から対向面側へ貫通するように設けられており、その貫通部がパッキン等のシーリング材でシールされている。
In the culture tank 21, a large number of translucent pipes 24, 24,... Penetrating the tank body 22 in the horizontal direction are arranged in parallel at regular intervals in the vertical and horizontal directions. It is formed into a cylindrical pipe with a high-strength acrylic resin and the like excellent in heat resistance and heat resistance, and is provided so as to penetrate from the one surface side of the rectangular cylindrical body portion 22a of the tank body 22 to the opposite surface side, The penetrating portion is sealed with a sealing material such as packing.
そして、培養タンク２１のタンク本体２２内に微細藻類の光合成を促す光を照射する照明装置２５は、タンク本体２２を水平方向に貫通する透光性パイプ２４、２４…の内部に抜き差し自在に差し込まれる直管型の蛍光ランプを光源２６としている。
And the illuminating device 25 which irradiates the tank main body 22 of the culture tank 21 with the light which promotes the photosynthesis of microalgae is inserted in the translucent pipes 24, 24 ... penetrating the tank main body 22 in the horizontal direction. A straight tube type fluorescent lamp is used as the light source 26.
また、培養タンク２１には、そのタンク本体２２の底部２２ｂに設けた排水口２８からタンク本体２２内の培養液Ｗの一部を循環ポンプ２９で抜き出して再びタンク本体２２内に還流させる培養液循環径路３０が設けられている。
In the culture tank 21, a part of the culture solution W in the tank body 22 is extracted by a circulation pump 29 from a drain port 28 provided in the bottom 22 b of the tank body 22, and is returned to the tank body 22 again. A circulation path 30 is provided.
培養液循環径路３０には、その径路を形成する配管３０ａとタンク本体２２内の培養液Ｗを外部に排出させるドレーン配管３１とをタンク本体２２の底部２２ａに設けられた排水口２８に対して切換接続する電磁三方弁３２と、タンク本体２２内の培養液Ｗの温度を検出する電子温度計３３から出力される温度検出信号に基づいて培養液の温度を調節する温度調節器３４と、微細藻類の光合成に必要な炭酸ガス（ＣＯ２）や微細藻類の生育・繁殖に必要な窒素等の栄養素を培養液Ｗ中に補給する培地補給装置３５と、ダイアフラムポンプ等からなる循環ポンプ２９とが介装されている。
The culture medium circulation path 30 includes a pipe 30 a that forms the path and a drain pipe 31 that discharges the culture medium W in the tank body 22 to the drain port 28 provided in the bottom 22 a of the tank body 22. An electromagnetic three-way valve 32 for switching connection, a temperature regulator 34 for adjusting the temperature of the culture medium based on a temperature detection signal output from an electronic thermometer 33 for detecting the temperature of the culture medium W in the tank body 22, and a fine A medium replenishing device 35 for replenishing the culture solution W with nutrients such as carbon dioxide (CO 2 ) necessary for the photosynthesis of algae and nitrogen necessary for the growth and reproduction of microalgae, and a circulation pump 29 comprising a diaphragm pump and the like It is intervened.
また、タンク本体２２の胴部２２ａには、培養液循環径路３０を通じて還流する培養液をその胴部２２ａの上方側から水平方向に吐出させる左右一対の上方側培養液吐出ノズル３６Ｒ、３６Ｌと、胴部２２ａの下方側から底部２２ｂと平行方向に吐出させる左右一対の下方側培養液吐出ノズル３７Ｒ、３７とが取り付けられて、タンク本体２２内の培養液Ｗを攪拌・流動させるようになっている。
The body 22a of the tank body 22 has a pair of left and right upper culture medium discharge nozzles 36R and 36L that discharge the culture medium refluxed through the culture medium circulation path 30 from the upper side of the body 22a in the horizontal direction. A pair of left and right lower culture medium discharge nozzles 37R and 37 for discharging from the lower side of the body 22a in a direction parallel to the bottom 22b is attached to stir and flow the culture medium W in the tank body 22. Yes.
以上の如く構成された藻類培養装置は、培養タンク２１のタンク本体２２内に光を照射する照明装置２５の光源２６、２６…が、タンク本体２２を貫通するように設けられた透光性パイプ２４、２４…の内部に抜き差し自在に差し込まれて培養タンク２１の外部に配置されているから、培養タンク２１の上蓋２３を閉じた状態で光源２６の交換作業や保守点検作業を行うことができ、その作業を行なう際に、培養タンク２１の内部に有害微生物や異物等が侵入してタンク本体２２内の培養液Ｗが汚染されるおそれもないという利点がある。
The algae culture apparatus configured as described above is a translucent pipe in which the light sources 26, 26,... Of the illuminating device 25 that irradiates light into the tank body 22 of the culture tank 21 are provided so as to penetrate the tank body 22. 24, 24... Are inserted into the inside of the culture tank 21 so as to be freely inserted and removed, so that the light source 26 can be exchanged or maintained while the top cover 23 of the culture tank 21 is closed. When performing the work, there is an advantage that harmful microorganisms, foreign substances, etc. enter the culture tank 21 and the culture solution W in the tank body 22 is not contaminated.
また、照明装置２５の光源２６から放射される光は、培養タンク２１のタンク本体２２内に一定の間隔で上下左右に並ぶように設けられた透光性パイプ２４の外周部からその周方向に沿って全方位的に放射されることにより、タンク本体２２内の胴部２２ａ側、底部２２ｂ側および液面側に向けて略均等に照射されることに加えて、タンク本体２２が、光反射率の高いステンレス鋼板等の金属板で製作されていることもあって、タンク本体２２内における光照射密度が全体的に略均一化するから、タンク本体２２内の培養液Ｗ中における微細藻類の培養濃度が高くても、タンク本体２２内全体にわたって微細藻類の光合成を促す光を均一に照射することが可能となる。
In addition, light emitted from the light source 26 of the illumination device 25 is transmitted in the circumferential direction from the outer peripheral portion of the translucent pipe 24 provided in the tank body 22 of the culture tank 21 so as to be aligned vertically and horizontally at regular intervals. In addition to being irradiated almost uniformly toward the body 22a side, the bottom 22b side and the liquid surface side in the tank main body 22, the tank main body 22 is reflected light by being omnidirectionally radiated along Since the light irradiation density in the tank body 22 is substantially uniform as a whole because it is made of a high-rate metal plate such as a stainless steel plate, the microalgae in the culture medium W in the tank body 22 Even if the culture concentration is high, it is possible to uniformly irradiate light that promotes photosynthesis of microalgae throughout the tank body 22.
更に、培養液循環径路３０を通じてタンク本体２２内へ還流する培養液が、タンク本体２２の胴部２２ａに取り付けられた各左右一対の上方側培養液吐出ノズル３６Ｒ、３６Ｌと下方側培養液吐出ノズル３７Ｒ、３７から水平方向に吐出されることにより、タンク本体２２内の培養液Ｗが攪拌・流動せられるので、照明装置２５の光源２６からの光が培養液Ｗ中の微細藻類に均一に照射されることとなる。
Furthermore, the culture medium that recirculates into the tank body 22 through the culture medium circulation path 30 is a pair of left and right upper culture liquid discharge nozzles 36R and 36L and a lower culture liquid discharge nozzle that are attached to the body 22a of the tank main body 22. Since the culture liquid W in the tank body 22 is agitated and fluidized by being discharged from 37R and 37 in the horizontal direction, the light from the light source 26 of the illumination device 25 is uniformly irradiated to the microalgae in the culture liquid W. Will be.
上記のような利点がある図２及び図３に示す従来装置は、大容量の培養タンク２１を用いて高品質の微細藻類を高効率・高濃度で大量培養することが可能であるとされているが、微細藻類の培養効率を高めるためには、培養タンク２１のタンク本体２２内に貯留された微細藻類の培養液Ｗ中に、その藻類の光合成に不可欠な炭酸ガス（ＣＯ２）を所要量注入・溶解させる必要がある。
The conventional apparatus shown in FIGS. 2 and 3 having the advantages as described above is capable of culturing a large amount of high-quality microalgae with high efficiency and high concentration using a large-capacity culture tank 21. However, in order to increase the culture efficiency of microalgae, carbon dioxide gas (CO 2 ) essential for photosynthesis of the algae is required in the culture liquid W of microalgae stored in the tank body 22 of the culture tank 21. It is necessary to inject and dissolve the amount.
そのため、タンク本体２２の底部２２ａから抜き出した培養液Ｗに培地補給装置３５で炭酸ガス（ＣＯ２）を注入し、培養液循環径路３０を通じてタンク本体２２内へ還流させているが、タンク本体２２の胴部２２ａの上方側に取り付けられた培養液吐出ノズル３６Ｒ、３６Ｌは、タンク本体２２内に還流する培養液を水平方向に吐出させるように配置されているため、吐出させた培養液中に含まれるＣＯ２の気泡が瞬時に培養液Ｗの液面に浮上して培養液中から抜け出てしまうので、照明装置２５により微細藻類の光合成を促す光が照射される培養液Ｗ中のＣＯ２溶解量を高めて微細藻類を高効率で大量培養することは困難であった。
Therefore, carbon dioxide (CO 2 ) is injected into the culture medium W extracted from the bottom 22 a of the tank body 22 by the medium supply device 35 and is refluxed into the tank body 22 through the culture medium circulation path 30. Since the culture solution discharge nozzles 36R and 36L attached to the upper side of the body portion 22a are disposed so as to discharge the culture solution refluxed into the tank body 22 in the horizontal direction, the culture solution discharge nozzles 36R and 36L are disposed in the discharged culture solution. Since the bubbles of CO 2 contained in the liquid instantly float on the surface of the culture solution W and escape from the culture solution, CO 2 in the culture solution W irradiated with light that promotes photosynthesis of microalgae by the lighting device 25 is emitted. It was difficult to cultivate microalgae with high efficiency by increasing the amount of dissolution.
また、本願の発明者が、タンク本体２２内の培養液Ｗ中におけるＣＯ２溶解量を高めて微細藻類を大量培養する実験を行ったところ、その藻類の光合成によって発生する酸素ガス（Ｏ２）が、タンク本体２２内の培養液Ｗの液面と、タンク本体２２の上面を閉塞する上蓋２３との間に生ずる空間内に多量に滞留・蓄積して、微細藻類の繁殖・生育を阻害する要因となることが判明した。
In addition, when the inventors of the present application conducted an experiment to increase the amount of CO 2 dissolved in the culture solution W in the tank body 22 to culture a large amount of microalgae, oxygen gas (O 2 ) generated by photosynthesis of the algae was obtained. However, it accumulates and accumulates in a large amount in the space formed between the liquid level of the culture medium W in the tank body 22 and the upper lid 23 that closes the upper surface of the tank body 22, thereby inhibiting the growth and growth of microalgae. It turned out to be a factor.
本発明は、微細藻類を高効率で大量培養するために、培養タンクのタンク本体内に貯留される培養液中のＣＯ２溶解量を高めると共に、そのＣＯ２溶解量を高めることで微細藻類の光合成によって発生する酸素ガス（Ｏ２）の量が増加し、その酸素ガスがタンク本体内の培養液の液面とタンク本体の上面を塞ぐ上蓋との間に生ずる空間内に多量に滞留・蓄積して、藻類の繁殖・生育が阻害されることを防止することを技術的課題としている。
The present invention, the microalgae to mass culture with high efficiency, of the culture tank to increase the CO 2 dissolved amount of culture medium which is stored in the tank body, that the microalgae increase its CO 2 dissolved amount The amount of oxygen gas (O 2 ) generated by photosynthesis increases, and the oxygen gas stays and accumulates in a large amount in the space formed between the liquid level of the culture solution in the tank body and the upper lid that closes the upper surface of the tank body. Therefore, it is a technical problem to prevent the algal breeding / growth from being inhibited.
上記課題を解決するために、本発明は、微細藻類の培養液が貯留される培養タンクのタンク本体内に微細藻類の光合成を促す光を照射する照明装置と、タンク本体内の培養液の一部を循環ポンプで抜き出してその液中に炭酸ガス（ＣＯ２）を補給注入して再びタンク本体内に還流させる培養液循環径路とを備えた藻類培養装置において、前記タンク本体に、前記培養液循環径路を通じて還流する培養液をタンク本体の胴部上方側から底部側に向けて吐出させる複数本の培養液吐出ノズルが取り付けられ、タンク本体の上面を閉塞する上蓋に、当該上蓋とタンク本体内に貯留された培養液の液面との間に生じた空間内に滞留する酸素ガス（Ｏ２）を外部に放出させる換気装置が取り付けられていることを特徴とする。
In order to solve the above-described problems, the present invention provides an illumination device that irradiates light that promotes photosynthesis of microalgae into a tank body of a culture tank in which a culture solution of microalgae is stored, and a culture solution in the tank body. An algae culture apparatus comprising a culture medium circulation path for extracting carbon dioxide (CO 2 ) into the liquid by injecting the part with a circulation pump and recirculating it into the tank main body. A plurality of culture solution discharge nozzles are attached to discharge the culture solution that circulates through the circulation path from the upper side to the bottom side of the body of the tank body, and the upper lid and the inside of the tank body are attached to the upper lid that closes the upper surface of the tank body. A ventilator for releasing oxygen gas (O 2 ) staying in a space formed between the liquid level of the culture solution stored in the outside is attached.
本発明によれば、培養タンクのタンク本体に取り付けられた培養液吐出ノズルによりタンク本体の胴部上方側から底部側に向けて吐出される培養液中に含まれる炭酸ガス（ＣＯ２）の気泡が、タンク本体内の培養液中を一旦タンク本体の底部側へ下降し、その底部側から上昇に転じて、タンク本体内の培養液の液面へと向かうこととなるので、タンク本体内の培養液中におけるＣＯ２の滞留時間が長くなってＣＯ２溶解量が高まり、微細藻類の光合成が促進される。
According to the present invention, bubbles of carbon dioxide (CO 2 ) contained in the culture solution discharged from the upper side of the trunk portion of the tank body toward the bottom side by the culture solution discharge nozzle attached to the tank body of the culture tank. However, the inside of the culture medium in the tank body is once lowered to the bottom side of the tank body and then turned upward from the bottom side to the liquid level of the culture medium in the tank body. The residence time of CO 2 in the culture solution becomes longer, the amount of CO 2 dissolved increases, and photosynthesis of microalgae is promoted.
また、微細藻類の光合成により発生してタンク本体内の培養液の液面とタンク本体の上蓋との間の空間内に滞留する酸素ガス（Ｏ２）が、上蓋に取り付けた換気装置により外部に放出されて、その酸素ガス（Ｏ２）により微細藻類の繁殖・生育が阻害されることが防止される。
In addition, oxygen gas (O 2 ) generated by photosynthesis of microalgae and staying in the space between the liquid level of the culture medium in the tank body and the upper lid of the tank body is released to the outside by a ventilation device attached to the upper lid. It is released and the oxygen gas (O 2 ) is prevented from inhibiting the growth and growth of microalgae.
本発明の実施形態に係る藻類培養装置は、ヘマトコッカス藻などの微細藻類の培養液が貯留される培養タンクのタンク本体内に微細藻類の光合成を促す光を照射する照明装置と、タンク本体内の培養液の一部を循環ポンプで抜き出してその液中に炭酸ガス（ＣＯ２）を補給注入して再びタンク本体内に還流させる培養液循環径路とを備え、タンク本体には、培養液循環径路を通じて還流する培養液をタンク本体の胴部上方側から底部側に向けて吐出させる複数本の培養液吐出ノズルが取り付けられ、タンク本体の上面を閉塞する上蓋には、当該上蓋とタンク本体内に貯留された培養液の液面との間に生じた上部空間内に滞留する高濃度の酸素ガス（Ｏ２）を外部に放出させる換気装置が取り付けられている。
An algae culture apparatus according to an embodiment of the present invention includes an illumination device that irradiates light that promotes photosynthesis of microalgae into a tank body of a culture tank in which a culture solution of microalgae such as Haematococcus algae is stored; A culture medium circulation path for extracting a part of the culture medium with a circulation pump, replenishing and injecting carbon dioxide (CO 2 ) into the liquid and recirculating it into the tank body. A plurality of culture solution discharge nozzles are attached to discharge the culture solution that circulates through the path from the upper side to the bottom side of the tank body, and the upper lid that closes the upper surface of the tank body A ventilation device is attached to discharge high-concentration oxygen gas (O 2 ) staying in the upper space generated between the culture solution stored in the chamber and the outside.
前記換気装置は、培養タンクのタンク本体の上面を閉塞する上蓋に取り付けられる排気口と、その排気口からタンク本体内の気体を放出させる換気ファンと、排気口を開閉する電動シャッターと、前記上蓋とタンク本体内に貯留された培養液の液面との間に生じた上部空間内に滞留する酸素ガス（Ｏ２）の濃度を計測して、そのＯ２濃度の計測値が一定値以上に達すると、換気ファンを起動させると同時に電動シャッターを開放させ、Ｏ２濃度の計測値が一定値以下に低下すると、換気ファンを停止させると同時に電動シャッターを閉鎖させる酸素計とを具備しており、当該酸素計は、培養タンクの上蓋に取り付けてタンク本体内のＯ２濃度を検出するＯ２センサを有している。
The ventilator includes an exhaust port attached to an upper lid that closes an upper surface of a tank body of a culture tank, a ventilation fan that discharges gas in the tank body from the exhaust port, an electric shutter that opens and closes the exhaust port, and the upper lid The concentration of oxygen gas (O 2 ) staying in the upper space generated between the liquid and the liquid level of the culture solution stored in the tank body is measured, and the measured value of the O 2 concentration exceeds a certain value. reached, and activates the ventilation fan opens the electric shutter simultaneously, the measured value of the O 2 concentration drops below a predetermined value, when stopping the ventilation fan is equipped with an oxygen meter for closing an electric shutter time The oximeter has an O 2 sensor that is attached to the upper lid of the culture tank and detects the O 2 concentration in the tank body.
また、前記換気装置の他の実施形態は、前記上蓋に取り付けられるφ25～φ70ｍｍの排気口と、当該排気口からタンク本体内の気体を常時放出させる小口径換気ファンとを具備している。
Further, another embodiment of the ventilation device includes an exhaust port of φ25 to φ70 mm attached to the upper lid, and a small-diameter ventilation fan that constantly discharges gas in the tank body from the exhaust port.
図１に示す本発明の実施例に係る藻類培養装置は、微細藻類の培養液Ｗが貯留される培養タンク１のタンク本体２とその上面を閉塞する上蓋３が、光反射率の高いステンレス鋼板で製作されている。そして、図示は省略するが、培養タンク１は、タンク本体２内に貯留される培養液Ｗ中に微細藻類の光合成を促す光を照射する照明装置を備えている。なお、当該照明装置は、直管型蛍光ランプを光源とする図２及び図３の照明装置と同型のものでもよいし、ＬＥＤや有機ＥＬ等を光源とするものでもよく、その構成は限定されない。
The algae culture apparatus according to the embodiment of the present invention shown in FIG. 1 is a stainless steel plate in which a tank main body 2 of a culture tank 1 in which a culture solution W of microalgae is stored and an upper lid 3 that closes the upper surface of the culture tank It is made with. And although illustration is abbreviate | omitted, the culture tank 1 is equipped with the illuminating device which irradiates the light which accelerates | stimulates the photosynthesis of a micro algae in the culture solution W stored in the tank main body 2. FIG. The illuminating device may be the same type as the illuminating device of FIGS. 2 and 3 using a straight tube fluorescent lamp as a light source, or may be an LED or organic EL as a light source, and its configuration is not limited. .
培養タンク１は、そのタンク本体２が、角筒状の胴部２ａと角型漏斗状の底部２ｂとで構成され、タンク本体２内に貯留された培養液Ｗの一部を循環ポンプ５で底部２ｂ側から抜き出し、その培養液中に炭酸ガス補給装置６でＣＯ２を補給注入して再びタンク本体２内に還流させる培養液循環径路４を備えている。
The culture tank 1 has a tank body 2 composed of a rectangular tube-shaped body 2 a and a square funnel-shaped bottom 2 b, and a circulation pump 5 removes a part of the culture solution W stored in the tank body 2. A culture solution circulation path 4 is provided which is extracted from the bottom 2b side, replenished and injected into the culture solution with CO 2 replenishment device 6 and recirculated into the tank body 2.
タンク本体２の胴部２ａの上方側と下方側には、培養液循環径路４を通じて還流する培養液をタンク本体２の底部２ｂ側に向けて斜め下方へ吐出させる各々左右一対の培養液吐出ノズル７Ｒ、７Ｌ及び８Ｒ、８Ｌが取り付けられている。
On the upper side and the lower side of the body 2a of the tank body 2, a pair of left and right culture solution discharge nozzles for discharging the culture solution refluxed through the culture solution circulation path 4 obliquely downward toward the bottom 2b side of the tank body 2 7R, 7L and 8R, 8L are attached.
これにより、培養液吐出ノズル７Ｒ、７Ｌ及び８Ｒ、８Ｌからタンク本体２内に吐出される培養液中に溶解したＣＯ２の気泡は、タンク本体２の底部２ｂ側へ一旦下降して、その底部２ｂ側から上昇に転じて、タンク本体２内の培養液Ｗの液面側へと浮上することとなるので、培養液Ｗ中における滞留時間が長くなってＣＯ２溶解量が高まり、微細藻類の光合成が促進される。
As a result, the CO 2 bubbles dissolved in the culture solution discharged from the culture solution discharge nozzles 7R, 7L and 8R, 8L into the tank body 2 once descend to the bottom 2b side of the tank body 2, Since it rises from the 2b side and floats to the liquid surface side of the culture medium W in the tank body 2, the residence time in the culture medium W becomes longer and the amount of dissolved CO 2 increases, and the microalgae Photosynthesis is promoted.
そして、タンク本体２の上面を閉塞する上蓋３には、当該上蓋３とタンク本体２内に貯留された培養液Ｗの液面との間に生じた空間に滞留する高濃度の酸素ガス（Ｏ２）を外部に放出させる換気装置１０が取り付けられている。
The upper lid 3 that closes the upper surface of the tank body 2 has a high-concentration oxygen gas (O) that stays in the space formed between the upper lid 3 and the liquid level of the culture medium W stored in the tank body 2. 2 ) A ventilator 10 for releasing the outside is attached.
換気装置１０は、培養タンク１の上蓋３に取り付けられる排気口１１と、その排気口１１からタンク本体２内の気体を放出させる換気ファン１２と、排気口１１を開閉する電動シャッター１３とを具備すると共に、培養タンク１の上蓋２とタンク本体２に貯留された培養液Ｗの液面との間に生じた空間内に滞留する酸素ガス（Ｏ２）の濃度を計測して、そのＯ２濃度の計測値が一定値以上に達すると、換気ファン１２を起動させると同時に電動シャッター１３を開放させ、Ｏ２濃度の計測値が一定値以下に低下すると、換気ファン１２を停止させると同時に電動シャッター１３を閉鎖させる制御信号を出力する酸素計１４を具備しており、当該酸素計１４には、培養タンクの上蓋に取り付けてタンク本体内のＯ２濃度を検出するＯ２センサ１５が設けられている。
The ventilation device 10 includes an exhaust port 11 attached to the upper lid 3 of the culture tank 1, a ventilation fan 12 that discharges gas in the tank body 2 from the exhaust port 11, and an electric shutter 13 that opens and closes the exhaust port 11. At the same time, the concentration of oxygen gas (O 2 ) staying in the space formed between the upper lid 2 of the culture tank 1 and the liquid level of the culture solution W stored in the tank body 2 is measured, and the O 2 is measured. When the measured value of the concentration reaches a certain value or more, the ventilation fan 12 is started and the electric shutter 13 is opened at the same time. When the measured value of the O 2 concentration falls below the certain value, the ventilation fan 12 is stopped and the motor is operated at the same time. and comprises an oxygen meter 14 which outputs a control signal for closing the shutter 13, to the oxygen meter 14, O 2 sensor for detecting the O 2 concentration in the tank body is attached to the upper lid of the culture tank 15 is provided.
しかして、微細藻類の光合成により発生してタンク本体２内の培養液の液面と上蓋３との間の空間内に滞留する酸素ガス（Ｏ２）が、上蓋３に取り付けた換気装置１０により外部に放出されて、その酸素ガス（Ｏ２）により微細藻類の繁殖・生育が阻害されることが防止される。また、換気装置１０は、換気ファン１２の起動・停止に応じて排気口１１を電動シャッター１３で開閉することにより、培養タンク１内へのゴミや虫などの異物の侵入を防止することができるものとなっている。
Thus, oxygen gas (O 2 ) generated by photosynthesis of microalgae and staying in the space between the liquid level of the culture solution in the tank body 2 and the upper lid 3 is caused by the ventilation device 10 attached to the upper lid 3. The oxygen gas (O 2 ) released to the outside is prevented from inhibiting the growth and growth of microalgae. In addition, the ventilator 10 can prevent entry of foreign matter such as dust and insects into the culture tank 1 by opening and closing the exhaust port 11 with the electric shutter 13 in accordance with the start / stop of the ventilation fan 12. It has become a thing.
なお、図示は省略するが、本発明の換気装置は、培養タンク１のタンク本体２の上面を閉塞する上蓋３に取り付けられるφ25～φ70ｍｍの排気口と、当該排気口からタンク本体２内の気体を常時放出させる小口径換気ファンとを具備したものであってもよく、当該小口径換気ファンを継続的に稼働させてタンク本体２内の気体を常時放出させれば、排気口からの異物の侵入も防止できる。
Although not shown in the drawings, the ventilator of the present invention has a φ25 to φ70 mm exhaust port attached to the upper lid 3 that closes the upper surface of the tank main body 2 of the culture tank 1, and the gas in the tank main body 2 from the exhaust port. A small-diameter ventilation fan that constantly discharges the gas, and if the small-diameter ventilation fan is continuously operated to release the gas in the tank body 2 constantly, the foreign matter from the exhaust port Intrusion can also be prevented.
１…培養タンク
２…タンク本体
２ａ…タンク本体の胴部
２ｂ…タンク本体の底部
３…上蓋
４…培養液循環径路
５…循環ポンプ
６…炭酸ガス補給装置
７Ｒ、７Ｌ…培養液吐出ノズル
８Ｒ、８Ｌ…培養液吐出ノズル
１０…換気装置
１１…排気口
１２…換気ファン
１３…電動シャッター
１４…酸素計
１５…Ｏ２センサ
Ｗ…培養液 DESCRIPTION OF SYMBOLS 1 ... Culture tank 2 ... Tankmain body 2a ... Tank main body 2b ... Tank main body 3 ... Upper cover 4 ... Culture solution circulation path 5 ... Circulation pump 6 ... Carbon dioxide supply device 7R, 7L ... Culture solution discharge nozzle 8R, 8L ... culture solution discharge nozzle 10 ... ventilator 11 ... exhaust port 12 ... ventilation fan 13 ... electric shutter 14 ... oxygen meter 15 ... O 2 sensor W ... culture
２…タンク本体
２ａ…タンク本体の胴部
２ｂ…タンク本体の底部
３…上蓋
４…培養液循環径路
５…循環ポンプ
６…炭酸ガス補給装置
７Ｒ、７Ｌ…培養液吐出ノズル
８Ｒ、８Ｌ…培養液吐出ノズル
１０…換気装置
１１…排気口
１２…換気ファン
１３…電動シャッター
１４…酸素計
１５…Ｏ２センサ
Ｗ…培養液 DESCRIPTION OF SYMBOLS 1 ... Culture tank 2 ... Tank
Claims (3)
- 微細藻類の培養液が貯留される培養タンクのタンク本体内に微細藻類の光合成を促す光を照射する照明装置と、タンク本体内の培養液の一部を循環ポンプで抜き出してその液中に炭酸ガス（ＣＯ２）を補給注入して再びタンク本体内に還流させる培養液循環径路とを備えた藻類培養装置において、前記タンク本体に、前記培養液循環径路を通じて還流する培養液をタンク本体の胴部上方側から底部側に向けて吐出させる複数本の培養液吐出ノズルが取り付けられ、タンク本体の上面を閉塞する上蓋に、当該上蓋とタンク本体内に貯留された培養液の液面との間に生じた空間内に滞留する酸素ガス（Ｏ２）を外部に放出させる換気装置が取り付けられていることを特徴とする藻類培養装置。 A lighting device that irradiates light in the tank body of the culture tank in which the culture solution of the microalgae is stored to promote photosynthesis of the microalgae, and a part of the culture solution in the tank body is extracted by a circulation pump, In an algae culture apparatus comprising a culture medium circulation path for replenishing and injecting gas (CO 2 ) and recirculating it into the tank body, the culture medium refluxed through the culture medium circulation path is supplied to the tank body. A plurality of culture solution discharge nozzles for discharging from the upper side to the bottom side of the unit are attached, and the upper lid that closes the upper surface of the tank body is between the upper lid and the liquid level of the culture solution stored in the tank body. A device for cultivating algae, wherein a ventilator for releasing oxygen gas (O 2 ) staying in the space generated in is released.
- 前記換気装置が、前記上蓋に取り付けられる排気口と、当該排気口から前記タンク本体内の気体を放出させる換気ファンと、排気口を開閉する電動シャッターと、前記上蓋とタンク本体に貯留された培養液の液面との間に生じた空間内に滞留する酸素ガス（Ｏ２）の濃度を計測して、そのＯ２濃度の計測値が一定値以上に達すると、前記換気ファンを起動させると同時に前記電動シャッターを開放させ、Ｏ２濃度の計測値が一定値以下に低下すると、換気ファンを停止させると同時に電動シャッターを閉鎖させる酸素計とを具備し、当該酸素計は、培養タンクの上蓋に取り付けてタンク本体内のＯ２濃度を検出するＯ２センサを有している請求項１記載の藻類培養装置。 The ventilator has an exhaust port attached to the upper lid, a ventilation fan for releasing the gas in the tank body from the exhaust port, an electric shutter for opening and closing the exhaust port, and a culture stored in the upper lid and the tank body When the concentration of oxygen gas (O 2 ) staying in the space generated between the liquid and the liquid surface is measured, and the measured value of the O 2 concentration reaches a certain value or more, the ventilation fan is started. At the same time, the electric shutter is opened, and when the measured value of the O 2 concentration drops below a certain value, an oxygen meter is provided that stops the ventilation fan and closes the electric shutter at the same time. The algae culture apparatus according to claim 1, further comprising an O 2 sensor that is attached to the tank body and detects an O 2 concentration in the tank body.
- 前記換気装置が、前記上蓋３に取り付けられるφ25～φ70ｍｍの排気口と、当該排気口から前記タンク本体内の気体を常時放出させる小口径換気ファンとを具備している請求項１記載の藻類培養装置。 The algae culture according to claim 1, wherein the ventilation device includes a φ25 to φ70 mm exhaust port attached to the upper lid 3 and a small-diameter ventilation fan that constantly discharges gas in the tank body from the exhaust port. apparatus.
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| JP2016-221325 | 2016-11-14 | ||
| JP2016221325A JP2020014385A (en) | 2016-11-14 | 2016-11-14 | Algae culture apparatus |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN109439515A (en) * | 2018-12-19 | 2019-03-08 | 广州甲宝机械有限公司 | A kind of spiral biological second level that declines spreads cultivation device and method |
| CN115851431A (en) * | 2023-02-14 | 2023-03-28 | 上海海圣生物实验设备有限公司 | Indoor alga culture device that uses |
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| JP7502919B2 (en) | 2020-03-25 | 2024-06-19 | 株式会社テヌート | Plant Cultivation Equipment |
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| JPH11239477A (en) * | 1998-02-25 | 1999-09-07 | Nakawaki Okimoto | Enrichment culture of microorganism raw material |
| JP2005169374A (en) * | 2003-12-11 | 2005-06-30 | Linde Kca Dresden Gmbh | Biological treatment method for suspension using bioreactor having liquid pressure type sediment takeout part and biological treatment apparatus therefor |
| WO2010116946A1 (en) * | 2009-04-06 | 2010-10-14 | マイクロリソース株式会社 | Microalgae cultivation device |
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2016
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH11239477A (en) * | 1998-02-25 | 1999-09-07 | Nakawaki Okimoto | Enrichment culture of microorganism raw material |
| JP2005169374A (en) * | 2003-12-11 | 2005-06-30 | Linde Kca Dresden Gmbh | Biological treatment method for suspension using bioreactor having liquid pressure type sediment takeout part and biological treatment apparatus therefor |
| WO2010116946A1 (en) * | 2009-04-06 | 2010-10-14 | マイクロリソース株式会社 | Microalgae cultivation device |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109439515A (en) * | 2018-12-19 | 2019-03-08 | 广州甲宝机械有限公司 | A kind of spiral biological second level that declines spreads cultivation device and method |
| CN115851431A (en) * | 2023-02-14 | 2023-03-28 | 上海海圣生物实验设备有限公司 | Indoor alga culture device that uses |
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| JP2020014385A (en) | 2020-01-30 |
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