KR101656748B1 - 탄화플루오르옥탄술폰산염 노출에 대응하는 바다송사리 유전자 및 이를 이용한 수생태계 환경오염 진단 방법 - Google Patents
탄화플루오르옥탄술폰산염 노출에 대응하는 바다송사리 유전자 및 이를 이용한 수생태계 환경오염 진단 방법 Download PDFInfo
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Abstract
본 발명은 탄화플루오르옥탄술폰산염(Perfluorooctane sulfonate; PFOS) 노출에 대응하는 바다송사리(Oryzias javanicus) 유전자 및 이를 이용하여 수생태계 환경오염을 진단하는 방법에 관한 것으로, 구체적으로 바다송사리를 배양하여 탄화플루오르옥탄술폰산염을 90 ㎍/L농도로 12, 24, 48, 및 72시간 동안 노출시킨 후, 이로부터 RNA를 분리하고, cDNA를 합성한 뒤, Cy3 및 Cy5로 표지하여 혼성화하였고, oligo-마이크로어래이를 제작하여 이를 분석한 결과, 12시간 노출군에서는 35종의 유전자(증가 17종, 감소 18종); 24시간 노출군에서는 60종의 유전자(증가 41종, 감소 19종); 48시간 노출군에서는 39종(증가 17종, 감소 22종); 72시간 노출군에서는 38종(증가 17종, 감소 21종)의 유전자 발현이 변화함을 확인하였고, 중복되는 유전자들을 제외한 총 126종(증가 68, 감소 58종)의 유전자들이 PFOS 노출에 의해 발현량이 변화됨을 확인함으로써, 이들 유전자들은 PFOS 노출을 확인할 수 있는 바이오마커로 유용하게 활용될 수 있다.
Description
본 발명은 탄화플루오르옥탄술폰산염(Perfluorooctane sulfonate; PFOS) 노출에 대응하는 바다송사리(Oryzias javanicus) 유전자 및 이를 이용하여 수생태계 환경오염을 진단하는 방법에 관한 것이다.
탄화플루오르옥탄술폰산염(Perfluorooctane sulfonate; PFOS) 및 탄화플루오르옥탄산(Perfluorooctanoic acid; PFOA)으로 대표되는 과불소화합물(Perfluorinated compounds, PFCs)은 우수한 발수성, 발유성, 방수성등의 특징으로 인해 표면 코팅제, 계면활성제등의 관련물질에 사용되고 있을 뿐 아니라, 발수성 의류, 화장품, 샴푸, 건축자재, 자동차 내장재, 주방용품의 코팅제등 광범위하게 사용되고 있는 물질이다. 이 물질은 분해가 되지 않으므로 환경내에서 잠재적 위험성이 크고, 최근 연구 결과 독성 및 생물농축성의 문제가 제기되어, EU를 비롯한 선진 각국에서는 PFOS 및 관련 화합물에 대한 규제를 예고하고 있다. 과불소화합물(PFCs: Perfluorinated Compounds)은 환경오염을 유발시키는 신종 오염물질로써 설치류나 원숭이 대상 실험에서 발암성이 입증되었고, 특히 수서생물의 내분비계를 교란함으로써 생물의 번식능에 치명적 손상을 초래할 뿐 아니라, 초기발생단계에 강한 독성을 나타내어 생물자원의 감소를 초래함으로써 수계생태계 파괴를 가중시키는 것으로 보고된 바 있다(Lau 등, 2004, Toxicol. Appl. Pharmacol. 198: 231-241). 최근 들어 PFOS가 스톡홀름협약 후보물질로 선정된 바 있고, 미국은 PFOS를 포함한 88종의 불소계화합물을 제조, 수입하는 경우 EPA에 통보하도록 하는 등 규제를 강화해 가고 있고, PFOS, PFOA 관련 물질들의 국내 유통량은 2002년 기준으로 약 120톤에 달한다. 과불소화화합물(PFCs)는 잔류성이 높은 새로운 환경오염물질군으로 알려져 있으며, 이들의 위해성이 특히 주목받고 있다. 이 화학물질들은 소화포말, 살충제 및 카펫, 가구, 종이 등의 표면처리제, 바닥 광택제, 윤활제 등으로 널리 이용되고 있다. PFOS는 환경내에서 가수분해, 광분해, 생물분해되지 않으며, 생물체내에 농축된다. 최근 PFCs의 생물 농축이 극지역에서도 확인되었으며 (Yamashita 등, 2005, Mar. Poll. Bull., 51: 658-668), 생식독성, 신경독성, 간독성 및 면역독성을 나타내며, 발암물질로 파악되고 있는 바(Kudo 와 Kawashima, 2003, J. Toxicol. Sci., 28: 49-57), 이들 화학물질의 위험성에 대한 우려가 높아지고 있다. 이와 같이 본 화학물질군에 대한 위험성이 심각한 수준이다.
생물들은 환경오염, 기후변화 및 병원성 미생물의 감염 등과 같은 외부 환경변화에 대응하여, 항상성을 유지하기 위해 생체방어기작을 진화시켜왔다. 이 생체방어기작의 대부분은 특정 유전자의 발현량을 능동적으로 변화시켜, 특정 단백질의 양을 조절하는 것으로 나타난다. 따라서 특정 변화에 대한 특이적인 유전자들을 발굴하여 이들의 발현량 변화를 모니터링한다면, 특정지역의 환경변화에 관한 정보뿐만 아니라, 이러한 환경변화가 생명현상에 미치는 영향 및 생태계의 건강에 관한 정보를 얻을 수 있다.
이에, 본 발명자들은 바다송사리를 대상으로 환경오염에 대응하는 생체지표의 발굴을 시도하였으며, PFOS 노출에 대한 특이 유전자 후보의 확보 및 검출을 위해, 송사리와 같은 속에 속하는 바다송사리(Oryzias javanicus) 유전자 해즈켐 피쉬 어레이(HazChem Fish Array)를 활용한, 마이크로어래이 실험분석을 통해, PFOS 12시간 노출군에서는 35종의 유전자(증가 17종, 감소 18종); 24시간 노출군에서는 60종의 유전자(증가 41종, 감소 19종); 48시간 노출군에서는 39종(증가 17종, 감소 22종); 72시간 노출군에서는 38종(증가 17종, 감소 21종)의 유전자 발현이 변화함을 확인하였고, 중복되는 유전자들을 제외한 총 126종(증가 68, 감소 58종)의 유전자들이 PFOS 노출에 의해 발현량이 변화됨을 확인함으로써, 이들 유전자들은 PFOS 노출을 확인할 수 있는 바이오마커로 활용하여, 탄화플루오르옥탄술폰산염 노출 여부를 확인할 수 있는 어류 생체지표 및 이들 유전자 생체지표들의 발현량 변화를 규명함으로써 본 발명을 완성하였다.
본 발명의 목적은 탄화플루오르옥탄술폰산염(Perfluorooctane sulfonate; PFOS) 노출에 대응하여 발현량이 변화된 바다송사리(Oryzias javanicus) 유전자 및 이를 이용한 탄화플루오르옥탄술폰산염 노출 여부를 확인하는 방법을 제공하는 것이다.
상기 목적을 달성하기 위하여, 본 발명은 서열번호 1 내지 126으로 기재되는 모든 유전자 각각의 핵산 서열의 올리고뉴클레오티드 전부 또는 그의 상보가닥 분자가 집적된, 시료내 탄화플루오르옥탄술폰산염(Perfluorooctane sulfonate; PFOS) 노출 여부 검출용 마이크로어레이 칩(microarray chip)을 제공한다.
또한, 본 발명은
1) 피검 시료에 노출된 실험군의 바다송사리와, 정상 대조군의 바다송사리에서 각각 RNA를 분리하는 단계;
2) 단계 1)의 실험군 및 대조군의 RNA로부터 cDNA를 합성하면서 실험군과 대조군을 각기 다른 형광물질로 표지하는 단계;
3) 단계 2)의 각기 다른 형광물질로 표지된 cDNA를 제 1항의 마이크로어레이 칩과 혼성화시키는 단계;
4) 반응한 마이크로어레이 칩을 분석하는 단계; 및
5) 분석한 데이터에서 제 1항의 마이크로어레이 칩에 집적된 유전자 발현 정도를 대조군과 비교하여 확인하는 단계를 포함하는, 시료내 탄화플루오르옥탄술폰산염(Perfluorooctane sulfonate; PFOS) 노출 여부 검출 방법을 제공한다.
또한, 본 발명은
1) 피검시료에 노출된 실험군의 바다송사리(Oryzias javanicus)와, 정상 대조군의 바다송사리에서 각각 RNA를 분리하는 단계;
2) 단계 1)의 RNA를, 서열번호 1 내지 126으로 기재되는 각각의 유전자에 상보적이고 유전자를 증폭할 수 있는 프라이머 쌍을 모두 사용하여 정량 실시간 RT-PCR(Quantitative real-time reverse transcript polymerase chain reaction, qRT-PCR)을 수행하는 단계; 및
3) 단계 2)의 유전자 산물을 대조군과 비교하여 발현 정도를 확인하는 단계를 포함하는, 시료내 탄화플루오르옥탄술폰산염(Perfluorooctane sulfonate; PFOS) 노출 여부 검출 방법을 제공한다.
또한, 본 발명은 상기 마이크로어레이 칩을 포함하는 탄화플루오르옥탄술폰산염(Perfluorooctane sulfonate; PFOS) 노출 여부 검출용 키트를 제공한다.
아울러, 본 발명은 서열번호 1 내지 126으로 기재되는 각각의 유전자에 상보적이고 유전자를 증폭할 수 있는 프라이머 쌍을 모두 포함하는 탄화플루오르옥탄술폰산염(Perfluorooctane sulfonate; PFOS) 노출 여부 검출용 키트를 제공한다.
본 발명에서 바다송사리(Oryzias javanicus)를 배양하여 탄화플루오르옥탄술폰산염(Perfluorooctane sulfonate; PFOS)을 90 ㎍/L농도로 12, 24, 48, 및 72시간 동안 노출시킨 후, 이로부터 RNA를 분리하고, cDNA를 합성한 뒤, Cy3 및 Cy5로 표지하여 혼성화하였고, oligo-마이크로어래이를 제작하여 이를 분석한 결과, 12시간 노출군에서는 35종의 유전자(증가 17종, 감소 18종); 24시간 노출군에서는 60종의 유전자(증가 41종, 감소 19종); 48시간 노출군에서는 39종(증가 17종, 감소 22종); 72시간 노출군에서는 38종(증가 17종, 감소 21종)의 유전자 발현이 변화함을 확인하였고, 중복되는 유전자들을 제외한 총 126종(증가 68, 감소 58종)의 유전자들이 PFOS 노출에 의해 발현량이 변화됨을 확인함으로써, 이들 유전자들은 PFOS 노출을 확인할 수 있는 바이오마커로 유용하게 활용될 수 있다.
도 1은 탄화플루오르옥탄술폰산염(Perfluorooctane sulfonate; PFOS)에 노출한 바다송사리(Oryzias javanicus)의 차등 발현 유전자 프로파일링 Hierachial clustering 결과를 나타낸 도이다. Fathead minnow 96-h LC50의 약 1/100에 해당하는 90 μg/L(ppb)의 농도로 12시간, 24시간, 48시간, 및 72시간 동안 노출시켰다.
이하, 본 발명을 상세히 설명한다.
본 발명은 탄화플루오르옥탄술폰산염(Perfluorooctane sulfonate; PFOS) 노출에 대응하여 발현이 변화하는 바다송사리(Oryzias javanicus) 유래의 유전자를 발굴하여, 상기 탄화플루오르옥탄술폰산염 노출에 대하여 발현량이 변화하는 바다송사리 유래 유전자를 집적한 마이크로어레이 칩을 탄화플루오르옥탄술폰산염 노출 여부 검출 및 수생태계 오염 상태를 진단하는데 이용할 수 있다.
본 발명은 서열번호 1 내지 126으로 기재되는 모든 유전자 각각의 핵산 서열의 올리고뉴클레오티드 전부 또는 그의 상보가닥 분자가 집적된, 시료내 탄화플루오르옥탄술폰산염 노출 여부 검출용 마이크로어레이 칩(microarray chip)을 제공한다.
상기 유전자는 바다송사리(Oryzias javanicus)로부터 유래된 것이 바람직하고, 서열번호 1 내지 58로 기재되는 유전자로 구성된 군으로부터 선택되는 유전자는 탄화플루오르옥탄술폰산염 노출에 대응하여 발현이 감소하는 것이 바람직하며, 서열번호 59 내지 126으로 기재되는 유전자로 구성된 군으로부터 선택되는 유전자는 탄화플루오르옥탄술폰산염 노출에 대응하여 발현이 증가하는 것이 바람직하다.
상기 탄화플루오르옥탄술폰산염 노출 여부 검출용 마이크로어레이 칩은 당업자에게 알려진 방법으로 제작할 수 있다. 상기 마이크로어레이 칩을 제작하는 방법은 하기와 같다. 상기 탐색된 유전자를 프로브로 이용하여 마이크로어레이 칩의 기판상에 고정화시키기 위해 파이조일렉트릭(piezoelectric) 방식을 이용한 마이크로피펫팅(micropipetting)법 또는 핀(pin) 형태의 스폿터(spotter)를 이용한 방법 등을 사용하는 것이 바람직하나 이에 한정되지 않는다. 상기 마이크로어레이 칩의 기판은 아미노-실란(amino-silane), 폴리-L-라이신(poly-Llysine) 및 알데히드(aldehyde)로 이루어진 군에서 선택되는 하나의 활성기가 코팅된 것이 바람직하나 이에 한정되지 않는다. 또한, 상기 기판은 슬라이드 글라스, 플라스틱, 금속, 실리콘, 나일론 막, 및 니트로셀룰로스 막으로 이루어진 군에서 선택될 수 있으나 이에 한정되지 않는다.
또한, 본 발명은
1) 피검 시료에 노출된 실험군의 바다송사리와, 정상 대조군의 바다송사리에서 각각 RNA를 분리하는 단계;
2) 단계 1)의 실험군 및 대조군의 RNA로부터 cDNA를 합성하면서 실험군과 대조군을 각기 다른 형광물질로 표지하는 단계;
3) 단계 2)의 각기 다른 형광물질로 표지된 cDNA를 제 1항의 마이크로어레이 칩과 혼성화시키는 단계;
4) 반응한 마이크로어레이 칩을 분석하는 단계; 및
5) 분석한 데이터에서 제 1항의 마이크로어레이 칩에 집적된 유전자 발현 정도를 대조군과 비교하여 확인하는 단계를 포함하는, 시료내 탄화플루오르옥탄술폰산염(Perfluorooctane sulfonate; PFOS) 노출 여부 검출 방법을 제공한다.
상기 시료는 생체시료, 식품시료, 화학시료, 공업시료, 임상시료 및 환경시료로 구성된 군으로부터 선택되는 어느 하나인 것이 바람직하고, 상기 단계 2)의 형광물질은 Cy3, Cy5, FITC(poly L-lysine-fluorescein isothiocyanate), RITC(rhodamine-B-isothiocyanate) 및 로다민(rhodamine)으로 이루어진 군으로부터 선택되는 어느 하나인 것이 바람직하다.
또한, 본 발명은
1) 피검시료에 노출된 실험군의 바다송사리(Oryzias javanicus)와, 정상 대조군의 바다송사리에서 각각 RNA를 분리하는 단계;
2) 단계 1)의 RNA를, 서열번호 1 내지 126으로 기재되는 각각의 유전자에 상보적이고 유전자를 증폭할 수 있는 프라이머 쌍을 모두 사용하여 정량 실시간 RT-PCR(Quantitative real-time reverse transcript polymerase chain reaction, qRT-PCR)을 수행하는 단계; 및
3) 단계 2)의 유전자 산물을 대조군과 비교하여 발현 정도를 확인하는 단계를 포함하는, 시료내 탄화플루오르옥탄술폰산염(Perfluorooctane sulfonate; PFOS) 노출 여부 검출 방법을 제공한다.
상기 시료는 생체시료, 식품시료, 화학시료, 공업시료, 임상시료 및 환경시료로 구성된 군으로부터 선택되는 어느 하나인 것이 바람직하다.
또한, 본 발명은 상기 마이크로어레이 칩을 포함하는 탄화플루오르옥탄술폰산염(Perfluorooctane sulfonate; PFOS) 노출 여부 검출용 키트를 제공한다.
상기 키트는 스트렙타비딘-알칼리 탈인화효소 접합물질(strepavidin -like phosphatease conjugate), 화학형광물질(chemiflurorensce) 및 화학발광물질(chemiluminescent)로 이루어진 형광물질군으로부터 선택되는 어느 하나를 추가적으로 포함하는 것이 바람직하고, 혼성화에 사용되는 완충용액, RNA로부터 cDNA(complementary DNA)를 합성하기 위한 역전사효소, dNTPs(deoxynucleotide triphosphates) 및 rNTPs(ribonucleotide triPhosphates, 사전 혼합형 또는 분리 공급형), 표식시약, 및 세척 완충용액으로 이루어진 반응 시약군으로부터 선택되는 어느 하나를 추가적으로 포함하는 것이 바람직하다.
아울러, 본 발명은 서열번호 1 내지 126으로 기재되는 각각의 유전자에 상보적이고 유전자를 증폭할 수 있는 프라이머 쌍을 모두 포함하는 탄화플루오르옥탄술폰산염(Perfluorooctane sulfonate; PFOS) 노출 여부 검출용 키트를 제공한다.
본 발명의 구체적인 실시예에서, 본 발명자들은 바다송사리를 배양하여 탄화플루오르옥탄술폰산염을 90 ㎍/L농도로 12, 24, 48, 및 72시간 동안 노출시킨 후, 이로부터 RNA를 분리하고, cDNA를 합성한 뒤, Cy3 및 Cy5로 표지하여 혼성화하였고, oligo-마이크로어래이를 제작하여 이를 분석한 결과, 12시간 노출군에서는 35종의 유전자(증가 17종, 감소 18종, 표 1 참조); 24시간 노출군에서는 60종의 유전자(증가 41종, 감소 19종, 표 2 참조); 48시간 노출군에서는 39종(증가 17종, 감소 22종, 표 3 참조); 72시간 노출군에서는 38종(증가 17종, 감소 21종, 표 4 참조)의 유전자 발현이 변화함을 확인하였고, 중복되는 유전자들을 제외한 총 126종(증가 68, 감소 58종, 표 5 및 표 6 참조)의 유전자들을 선별하였다.
따라서, 상기 유전자들을 포함하는 바이오마커를 프로브로 이용하여 피검체와 대조군의 RNA를 추출하여 상기 마커 유전자들의 발현 변화를 비교분석함으로써, 피검체가 탄화플루오르옥탄술폰산염에 노출되었는지 여부를 확인할 수 있다. 즉, 만약 피검체가 탄화플루오르옥탄술폰산염에 노출되었다면, 표 6에 기재되어 있는 유전자들의 발현량이 대조군에 비해 피검체에서 증가한 것으로 나타날 것이고, 표 5에 기재되어 있는 유전자들의 발현량은 대조군에 비해 피검체에서 감소한 것으로 나타날 것이므로, 이를 이용하여 피검체의 탄화플루오르옥탄술폰산염의 노출 여부를 확인할 수 있다.
이하, 본 발명을 실시예에 의해서 상세히 설명한다.
단, 하기 실시예는 본 발명을 예시하기 위한 것일 뿐, 본 발명이 하기 실시예에 의해서 한정되는 것은 아니다.
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실시예
1> 송사리의 배양 및
PFOS
노출
<1-1> 송사리의 배양
송사리는 세 종류의 필터(100, 10 및 1 ㎛, Spun Polypropylene filter cartilage, Heathy)를 거친 해수에 배양하였다. 그런 다음, 수중 히터를 이용하여 수온은 섭씨 25도로 고정하였으며, 하루에 한 번 아르테미아(Artemia) 유생을 먹이로 공급하였다.
<1-2>
PFOS
노출 조건 확립
배양 수조에서 사육중인 송사리 수컷을 여과된 해수 2 L가 담긴 3 L 비이커에 10마리씩 옮기고, 24시간 동안 순치시켰다. 그 이후, 송사리 수컷을 한 그룹 당 5개체씩 탄화플루오르옥탄술폰산염(Perfluorooctane sulfonate; PFOS)을 90 ㎍/L농도로 12, 24, 48, 및 72시간 동안 노출시켰다. 상기 노출 농도는 바다송사리의 PFOS 반수치사농도(Lethal concentration 50, LC50, 9.1 mg/L 96시간)의 약 1/100에 해당되는 매우 낮은 수준의 농도이다. PFOS 노출 이후, 송사리를 한 마리씩 얼음물로 옮겨, 순간적으로 기절시킨 후, 머리를 잘라내고, 배를 갈라, 간을 적출하여 하기 실험에 사용하였다.
<
실시예
2>
PFOS
노출에 의한 유전자 발현량 변화 측정
<2-1> RNA 추출 및 cDNA 합성
상기 실시예 <1-2>에서 수확한 PFOS 노출 시험군(12, 24, 48, 72시간) 및 노출하지 않은 대조군의 송사리 간에 TRI Reagent 용액(Molecular Research Center Inc, Cincinnati, Ohaio, USA) 1 ㎖를 넣고, 유리 균질기(glass homogenizer)를 이용하여 균질화한 뒤, 실온에 5분간 방치하였다. 그런 다음, 클로로포름 200 ㎕를 첨가하여 혼합하였고, 실온에서 10분간 방치한 후, 15분간 원심분리(12,000g, 4℃)하였다. 원심분리 후의 상층액을 수득하여, 아이소프로판올(isopropanol) 500 ㎕를 넣고, 상온에 5분간 방치하였고, 다시 약 20분간 원심분리(12,000×g, 4℃)한 후, 용액을 제거하여 침전물만을 수확하였다. 상기 침전물에 70% 에탄올 용액 50 ㎕를 넣어 5분간 원심분리(12,000×g, 4℃)한 뒤, 에탄올 용액을 제거하고 침전된 RNA를 건조한 후, 적당량의 디에틸피로카보네이트(diethylpyrocarbonate, DEPC)로 처리한 멸균수에 용해하였다.
<2-2>
Cy3
및
Cy5
표지
정제된 노출군 및 대조군의 전체 RNA는 Agilent's Low RNA Input Linear Amplification Kit Plus(Agilent Technologies, USA)를 사용하여 제조사의 프로토콜을 따라 다음과 같이 Cy3 및 Cy5로 표지하였다.
구체적으로, RNA 1 ㎍을 dT-프로모터 프라이머(dT-promoter primer)와 MMLV-역전사효소(MMLV-Reverse transcriptase)와 혼합하여 40℃에서 2시간 동안 역전사반응을 수행하였다. 그런 다음, T7 중합효소(T7 polymerase)를 첨가하여 40℃에서 2시간 동안 선형 증폭(linear amplification)을 수행하였다. 이와 같은 증폭과정을 통해 실험군 및 대조군의 시료를 각각 Cy3-CTP와 Cy5-CTP로 표지하였다.
<2-3>
혼성화
(Hybridization) 및 스캐닝(scanning)
형광물질이 라벨링된 cRNA 시료를 Qiagen PCR purification kit을 사용하여 정제하고, 증류수로 용출하였다. 그 후, 정제된 형광표지-cRNA 시료를 혼성화 완충액(hybridization buffer)(3×SSC, 0.3% SDS, 50% 포름아미드(formamide), 20 ㎍ Cot-1 DNA, 20 ㎍ 효모균(yeast) tRNA)에 첨가한 후, microcon YM-30으로 농축하여 혼성화 혼합물을 만들었다. 혼성화 혼합물은 95℃로 3분 동안 가열하여 변성시키고 12,000×g에서 30초간 원심분리하며 온도를 낮추었다. 제조된 바다송사리 oligo-마이크로어래이(microarray, HazChem Fish Array)에 커버슬립(coverslip)을 덮고, 변성시킨 혼성화 혼합물을 파이펫팅(pipetting)하였다. 그런 다음, 상기 마이크로어래이를 GT-Hyb 챔버(Chamber)에 넣고 65℃에서 16시간 동안 반응시켜 혼성화 하였다. 혼성화가 끝난 후, 챔버에서 마이크로어래이를 꺼내어 세척과정을 수행하였고, 마이크로어래이를 회전하여 건조한 후 스캐닝 할 때까지 암실에서 보관하였다. 실험이 완료된 HazChem Fish Array를 Axon GenePix 4000B scanner(Axon Instrument, USA)를 사용하여 스캔하였으며, GenePix Pro 6.0 program을 이용하여, 스캔 이미지로부터 각 점을 그리딩 파일(gridding file)을 이용하여 그리딩하고, 정량화하여 각 점의 Cy5/Cy3 강도 및 비율등의 분석값이 포함된 GPR 파일(GPR file)을 얻었다.
<
실시예
3>
마이크로어래이
자료 분석
GenePix Pro Program에서 얻어진 GPR 파일로부터, 분석 프로그램인 GeneSpring 7.3.1(Agilent Technologies, USA)를 이용하여 아래와 같이 분석을 수행하였다:
표준화(Normalization)는 LOWESS(locally weighted regression scatterplot smoothing)를 이용하여 수행하였고, 신뢰할 수 있는 유전자(Reliable gene)는 중앙값의 합이 배경(background) 보다 낮거나, 각 화소(pixel) 값의 표준편차가 유의하지 않은 점을 플래그 아웃(flag-out)함으로써 유의한 유전자를 얻었다. 유의한 유전자(Significant genes)는 평준화된 비율(normalized ratio) 값이 10배 이상 차이를 보이는 점을 선별하였다.
<3-1> 특이유전자후보군의 확인
1차적으로 PFOS 노출 시간별 유전자 발현량이 2배 이상 변화되는 유전자들을 선별하였다. 노출 실험은 3회 실시하였으며, 통계적으로 유의한 유전자들을 선별하였다.
그 결과, 12시간 노출군에서는 35종의 유전자(증가 17종, 감소 18종; 표 1); 24시간 노출군에서는 60종의 유전자(증가 41종, 감소 19종; 표 2); 48시간 노출군에서는 39종(증가 17종, 감소 22종; 표 3); 72시간 노출군에서는 38종(증가 17종, 감소 21종; 표 4)의 유전자 발현이 변화함을 확인하였다. 이들 중 중복되는 유전자들을 제외하면, 표 5에 나타난 바와 같이, 총 127종의 유전자들이 PFOS 노출에 의해 발현량이 변화되었으며(표 5), 따라서 이들 유전자들은 PFOS 노출을 확인할 수 있는 바이오마커로 활용 가능함을 확인하였다.
| 유전자 | 실험 1 | 실험 2 | 실험 3 | |
| hemoglobin subunit beta-like [Oryzias javanicus] | 2.043 | 1.990 | 2.097 | |
| 15-hydroxyprostaglandin dehydrogenase [NAD(+)]-like [Oryzias javanicus] | 0.494 | 0.461 | 0.529 | |
| 39S ribosomal protein L12, mitochondrial-like [Oryzias javanicus] | 0.443 | 0.401 | 0.490 | |
| ADP-ribosylation factor-like protein 5C-like [Oryzias javanicus] | 0.397 | 0.450 | 0.351 | |
| apolipoprotein C-II-like isoform X1 [Oryzias javanicus] | 2.804 | 2.570 | 3.058 | |
| apolipoprotein Eb-like [Oryzias javanicus] | 52.460 | 54.282 | 50.698 | |
| beta-2-glycoprotein 1-like [Oryzias javanicus] | 0.479 | 0.439 | 0.523 | |
| cadherin-11-like, partial [Oryzias javanicus] | 2.213 | 1.897 | 2.580 | |
| creatine kinase B-type-like [Oryzias javanicus] | 2.496 | 2.927 | 2.129 | |
| DNA damage-inducible transcript 4 protein-like [Oryzias javanicus] | 0.274 | 0.311 | 0.241 | |
| fibulin-2-like [Oryzias javanicus] | 11.492 | 9.978 | 13.236 | |
| hemoglobin subunit beta-like [Oryzias javanicus] | 2.245 | 2.626 | 1.919 | |
| hepcidin-like [Oryzias javanicus] | 0.206 | 0.223 | 0.189 | |
| inositol oxygenase-like [Oryzias javanicus] | 0.240 | 0.199 | 0.289 | |
| interferon-induced protein 44-like [Oryzias javanicus] | 0.429 | 0.531 | 0.346 | |
| kelch-like protein 12-like isoform X3 [Oryzias javanicus] | 2.023 | 2.030 | 2.016 | |
| ketohexokinase-like [Oryzias javanicus] | 0.464 | 0.473 | 0.455 | |
| LOW QUALITY PROTEIN: selenoprotein H-like [Oryzias javanicus] | 2.046 | 2.220 | 1.885 | |
| lysyl oxidase homolog 2A-like [Oryzias javanicus] | 0.395 | 0.469 | 0.332 | |
| microfibril-associated glycoprotein 4-like [Oryzias javanicus] | 4.864 | 6.519 | 3.630 | |
| myb-binding protein 1A-like protein-like [Oryzias javanicus] | 8.172 | 9.956 | 6.708 | |
| nidogen-2-like [Oryzias javanicus] | 4.993 | 4.081 | 6.110 | |
| nuclear distribution protein nudE-like 1-B-like [Oryzias javanicus] | 0.415 | 0.383 | 0.450 | |
| palmitoyl-protein thioesterase 1-like [Oryzias javanicus] | 0.305 | 0.316 | 0.295 | |
| phosphoglycerate mutase 1-like [Oryzias javanicus] | 0.285 | 0.253 | 0.321 | |
| pleiotrophin-like [Oryzias javanicus] | 2.555 | 2.531 | 2.578 | |
| rab3 GTPase-activating protein non-catalytic subunit-like [Oryzias javanicus] | 4.011 | 3.553 | 4.528 | |
| ras association domain-containing protein 7-like [Oryzias javanicus] | 0.494 | 0.538 | 0.454 | |
| retinol dehydrogenase 7-like [Oryzias javanicus] | 2.955 | 3.102 | 2.815 | |
| SH3 domain and tetratricopeptide repeat-containing protein 2-like [Oryzias javanicus] | 2.258 | 2.548 | 2.002 | |
| sodium-coupled neutral amino acid transporter 2-like [Oryzias javanicus] | 0.489 | 0.433 | 0.551 | |
| sodium-dependent phosphate transporter 1-B-like [Oryzias javanicus] | 0.433 | 0.445 | 0.422 | |
| thyroid transcription factor 1-associated protein 26 homolog [Oryzias javanicus] | 0.472 | 0.408 | 0.547 | |
| transcription factor AP-2-alpha-like [Oryzias javanicus] | 2.621 | 2.248 | 3.055 | |
| uridine phosphorylase 2-like [Oryzias javanicus] | 0.412 | 0.428 | 0.396 | |
(측정 실험은 3번 반복하여 실시하였고, 측정값이 1보다 작으면 발현량이 감소, 1보다 크면 발현량이 증가한 것이다.)
| 유전자 | 실험 1 | 실험 2 | 실험 3 |
| brain-type fatty acid binding protein [Oryzias javanicus] | 35.021 | 55.907 | 21.937 |
| choriogenin H precursor [Oryzias javanicus] | 0.415 | 0.349 | 0.495 |
| cysteine-rich protein 1 (intestinal) [Oryzias javanicus] | 2.522 | 2.611 | 2.436 |
| fatty acid binding protein 7, brain, a [Oryzias javanicus] | 35.896 | 49.406 | 26.080 |
| Guanine nucleotide-binding protein GI/GS/GO subunit gamma-2 [Oryzias javanicus] | 2.146 | 1.982 | 2.324 |
| Mhc class I A precursor [Oryzias javanicus] | 2.125 | 2.549 | 1.771 |
| mitochondrial uncoupling protein 3 [Oryzias javanicus] | 2.538 | 2.720 | 2.368 |
| Na/K ATPase alpha subunit isoform 1b [Oryzias javanicus] | 2.199 | 2.044 | 2.366 |
| acidic mammalian chitinase-like [Oryzias javanicus] | 41.929 | 41.112 | 42.762 |
| AN1-type zinc finger protein 5-like isoform 1 [Oryzias javanicus] | 0.225 | 0.240 | 0.212 |
| apolipoprotein A-IV-like [Oryzias javanicus] | 6.726 | 6.378 | 7.092 |
| apolipoprotein Eb-like [Oryzias javanicus] | 59.765 | 51.494 | 69.366 |
| beta-galactoside-binding lectin-like [Oryzias javanicus] | 10.424 | 12.100 | 8.980 |
| carnitine O-acetyltransferase-like [Oryzias javanicus] | 2.049 | 2.166 | 1.938 |
| caspase-6-like [Oryzias javanicus] | 4.450 | 3.843 | 5.152 |
| c-C motif chemokine 20-like [Oryzias javanicus] | 2.003 | 1.942 | 2.065 |
| coagulation factor XI-like [Oryzias javanicus] | 0.476 | 0.425 | 0.533 |
| coiled-coil domain-containing protein 43-like isoform 1 [Oryzias javanicus] | 0.436 | 0.550 | 0.346 |
| complement C1q-like protein 2-like [Oryzias javanicus] | 0.349 | 0.405 | 0.301 |
| creatine kinase B-type-like [Oryzias javanicus] | 3.872 | 3.056 | 4.905 |
| cytochrome P450 2D10-like isoform 1 [Oryzias javanicus] | 0.422 | 0.483 | 0.369 |
| dehydrogenase/reductase SDR family member 11-like [Oryzias javanicus] | 2.366 | 2.131 | 2.628 |
| deleted in malignant brain tumors 1 protein-like [Oryzias javanicus] | 18.175 | 18.332 | 18.019 |
| fibulin-2-like [Oryzias javanicus] | 12.904 | 10.190 | 16.340 |
| FYVE, RhoGEF and PH domain-containing protein 3-like [Oryzias javanicus] | 2.101 | 1.759 | 2.509 |
| glycogen debranching enzyme-like [Oryzias javanicus] | 0.485 | 0.540 | 0.435 |
| GTP cyclohydrolase 1-like [Oryzias javanicus] | 2.179 | 2.169 | 2.188 |
| GTP:AMP phosphotransferase, mitochondrial-like [Oryzias javanicus] | 2.539 | 2.543 | 2.535 |
| h-2 class II histocompatibility antigen, I-A beta chain-like [Oryzias javanicus] | 3.961 | 3.727 | 4.210 |
| heme oxygenase-like [Oryzias javanicus] | 2.126 | 2.273 | 1.989 |
| heterogeneous nuclear ribonucleoprotein A/B-like [Oryzias javanicus] | 0.394 | 0.353 | 0.441 |
| insulin-induced gene 1 protein-like [Oryzias javanicus] | 3.112 | 3.558 | 2.723 |
| kelch-like protein 12-like isoform X3 [Oryzias javanicus] | 2.305 | 2.753 | 1.929 |
| KH domain-containing, RNA-binding, signal transduction-associated protein 1-like [Oryzias javanicus] | 0.401 | 0.472 | 0.340 |
| methyl-CpG-binding domain protein 3-like [Oryzias javanicus] | 0.435 | 0.350 | 0.540 |
| microfibril-associated glycoprotein 4-like [Oryzias javanicus] | 27.448 | 24.386 | 30.895 |
| microfibril-associated glycoprotein 4-like [Oryzias javanicus] | 11.008 | 13.200 | 9.180 |
| motile sperm domain-containing protein 1-like isoform X1 [Oryzias javanicus] | 2.331 | 2.266 | 2.397 |
| myelin proteolipid protein-like isoform 1 [Oryzias javanicus] | 2.243 | 2.102 | 2.394 |
| NEDD8-activating enzyme E1 catalytic subunit-like isoform X1 [Oryzias javanicus] | 3.141 | 3.104 | 3.178 |
| nidogen-2-like [Oryzias javanicus] | 5.503 | 5.161 | 5.869 |
| nuclear distribution protein nudE-like 1-B-like [Oryzias javanicus] | 0.482 | 0.493 | 0.472 |
| nucleoplasmin-like protein NO29-like [Oryzias javanicus] | 0.440 | 0.476 | 0.406 |
| pancreatic progenitor cell differentiation and proliferation factor A-like isoform 1 [Oryzias javanicus] | 0.240 | 0.258 | 0.223 |
| pleckstrin homology domain-containing family A member 6-like [Oryzias javanicus] | 0.466 | 0.391 | 0.557 |
| pleiotrophin-like [Oryzias javanicus] | 2.459 | 2.780 | 2.174 |
| putative hexokinase HKDC1-like [Oryzias javanicus] | 5.354 | 4.448 | 6.445 |
| pyruvate dehydrogenase [lipoamide] kinase isozyme 2-like [Oryzias javanicus] | 2.053 | 1.919 | 2.197 |
| rab3 GTPase-activating protein non-catalytic subunit-like [Oryzias javanicus] | 3.820 | 3.385 | 4.310 |
| ras association domain-containing protein 7-like [Oryzias javanicus] | 0.482 | 0.520 | 0.448 |
| serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit delta isoform-like [Oryzias javanicus] | 2.012 | 2.253 | 1.796 |
| SH3 domain and tetratricopeptide repeat-containing protein 2-like [Oryzias javanicus] | 2.379 | 2.256 | 2.508 |
| signal peptidase complex subunit 3-like [Oryzias javanicus] | 0.478 | 0.576 | 0.396 |
| sodium- and chloride-dependent taurine transporter-like [Oryzias javanicus] | 2.650 | 3.191 | 2.200 |
| spermidine synthase-like [Oryzias javanicus] | 0.482 | 0.565 | 0.411 |
| sphingomyelin phosphodiesterase 2-like [Oryzias javanicus] | 2.422 | 2.069 | 2.836 |
| thyroid transcription factor 1-associated protein 26 homolog [Oryzias javanicus] | 0.392 | 0.373 | 0.413 |
| ubiquitin-like protein-like [Oryzias javanicus] | 2.567 | 2.554 | 2.580 |
| zinc finger and BTB domain-containing protein 1-like [Oryzias javanicus] | 0.349 | 0.417 | 0.291 |
| Ubiquitin-like protein [Oryzias javanicus] | 4.160 | 5.240 | 3.302 |
| 유전자 | 실험1 | 실험2 | 실험3 |
| actin, alpha, cardiac muscle 1a [Oryzias javanicus] | 0.453 | 0.519 | 0.395 |
| choriogenin H precursor [Oryzias javanicus] | 0.423 | 0.459 | 0.390 |
| mitochondrial uncoupling protein 3 [Oryzias javanicus] | 2.788 | 3.099 | 2.508 |
| NEF1 protein [Oryzias javanicus] | 0.486 | 0.560 | 0.423 |
| 40S ribosomal protein S16-like isoform X1 [Oryzias javanicus] | 2.039 | 2.341 | 1.775 |
| 5-aminolevulinate synthase, erythroid-specific, mitochondrial isoform 1 [Oryzias javanicus] | 2.892 | 3.547 | 2.358 |
| actin, cytoplasmic-like [Oryzias javanicus] | 0.466 | 0.536 | 0.406 |
| ADP-ribosylation factor-like protein 5C-like [Oryzias javanicus] | 0.332 | 0.366 | 0.302 |
| ankyrin-1-like [Oryzias javanicus] | 2.315 | 2.593 | 2.067 |
| apolipoprotein A-IV-like [Oryzias javanicus] | 2.053 | 1.880 | 2.242 |
| c-C motif chemokine 20-like [Oryzias javanicus] | 2.977 | 3.435 | 2.581 |
| cholinephosphotransferase 1-like [Oryzias javanicus] | 2.179 | 1.859 | 2.556 |
| coiled-coil domain-containing protein 109B-like, partial [Oryzias javanicus] | 0.468 | 0.475 | 0.461 |
| coiled-coil domain-containing protein 43-like isoform 1 [Oryzias javanicus] | 0.395 | 0.504 | 0.310 |
| DNA damage-inducible transcript 4 protein-like [Oryzias javanicus] | 0.251 | 0.264 | 0.238 |
| eukaryotic translation initiation factor 3 subunit C-like, partial [Oryzias javanicus] | 0.440 | 0.512 | 0.379 |
| fatty acid desaturase 2-like [Oryzias javanicus] | 0.449 | 0.391 | 0.515 |
| fibulin-2-like [Oryzias javanicus] | 15.640 | 14.231 | 17.189 |
| fructose-bisphosphate aldolase B-like [Oryzias javanicus] | 2.243 | 1.869 | 2.693 |
| iron-sulfur cluster assembly enzyme ISCU, mitochondrial-like isoform 1 [Oryzias javanicus] | 0.467 | 0.517 | 0.422 |
| kelch-like protein 12-like isoform X3 [Oryzias javanicus] | 2.156 | 2.130 | 2.182 |
| kinesin heavy chain isoform 5C-like [Oryzias javanicus] | 2.168 | 2.635 | 1.784 |
| leucine--tRNA ligase, cytoplasmic-like [Oryzias javanicus] | 0.391 | 0.480 | 0.319 |
| lysyl oxidase homolog 2A-like [Oryzias javanicus] | 0.403 | 0.388 | 0.418 |
| mitochondrial uncoupling protein 2-like isoform X1 [Oryzias javanicus] | 2.452 | 2.506 | 2.400 |
| multiple coagulation factor deficiency protein 2-like [Oryzias javanicus] | 0.447 | 0.469 | 0.426 |
| nidogen-2-like [Oryzias javanicus] | 5.960 | 6.443 | 5.513 |
| pancreatic progenitor cell differentiation and proliferation factor A-like isoform 1 [Oryzias javanicus] | 0.377 | 0.314 | 0.452 |
| peptidyl-prolyl cis-trans isomerase FKBP2-like [Oryzias javanicus] | 0.445 | 0.460 | 0.430 |
| pirin-like [Oryzias javanicus] | 0.478 | 0.522 | 0.439 |
| pleckstrin homology domain-containing family A member 6-like [Oryzias javanicus] | 0.444 | 0.411 | 0.480 |
| probable ATP-dependent RNA helicase DDX5-like [Oryzias javanicus] | 0.483 | 0.484 | 0.483 |
| rab3 GTPase-activating protein non-catalytic subunit-like [Oryzias javanicus] | 4.333 | 4.030 | 4.659 |
| ras association domain-containing protein 7-like [Oryzias javanicus] | 0.425 | 0.362 | 0.499 |
| retinol dehydrogenase 12-like [Oryzias javanicus] | 0.457 | 0.540 | 0.387 |
| sodium- and chloride-dependent taurine transporter-like [Oryzias javanicus] | 2.012 | 1.755 | 2.305 |
| thyroid transcription factor 1-associated protein 26 homolog [Oryzias javanicus] | 0.484 | 0.579 | 0.405 |
| translation initiation factor eIF-2B subunit beta-like [Oryzias javanicus] | 2.049 | 1.840 | 2.281 |
| transmembrane protein 230-like [Oryzias javanicus] | 2.113 | 2.485 | 1.796 |
| 유전자 | 실험 1 | 실험 2 | 실험 3 |
| beclin 1 [Oryzias javanicus] | 0.347 | 0.317 | 0.379 |
| mitochondrial uncoupling protein 3 [Oryzias javanicus] | 3.575 | 3.422 | 3.735 |
| Na/K ATPase alpha subunit isoform 1b [Oryzias javanicus] | 2.067 | 1.880 | 2.273 |
| 1,4-alpha-glucan-branching enzyme-like [Oryzias javanicus] | 2.017 | 1.706 | 2.384 |
| 14 kDa phosphohistidine phosphatase-like [Oryzias javanicus] | 0.459 | 0.494 | 0.426 |
| 5-aminolevulinate synthase, nonspecific, mitochondrial-like [Oryzias javanicus] | 2.236 | 2.712 | 1.843 |
| ADP-ribosylation factor-like protein 5C-like [Oryzias javanicus] | 0.124 | 0.150 | 0.103 |
| AN1-type zinc finger protein 5-like isoform 1 [Oryzias javanicus] | 0.196 | 0.237 | 0.162 |
| anti-apoptotic protein NR13-like [Oryzias javanicus] | 0.434 | 0.506 | 0.372 |
| chaperone activity of bc1 complex-like, mitochondrial-like [Oryzias javanicus] | 2.033 | 2.003 | 2.063 |
| coagulation factor XI-like [Oryzias javanicus] | 0.413 | 0.337 | 0.507 |
| complement C1q-like protein 2-like [Oryzias javanicus] | 0.200 | 0.167 | 0.240 |
| DNA damage-inducible transcript 4 protein-like [Oryzias javanicus] | 0.065 | 0.058 | 0.074 |
| dnaJ homolog subfamily C member 15-like isoform X1 [Oryzias javanicus] | 0.394 | 0.405 | 0.383 |
| dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48 kDa subunit-like [Oryzias javanicus] | 0.496 | 0.577 | 0.426 |
| fructose-bisphosphate aldolase B [Oryzias javanicus] | 2.111 | 2.230 | 1.997 |
| fructose-bisphosphate aldolase B-like isoform X2 [Oryzias javanicus] | 2.249 | 2.120 | 2.386 |
| GTP cyclohydrolase 1-like [Oryzias javanicus] | 3.354 | 3.896 | 2.887 |
| heterogeneous nuclear ribonucleoprotein A/B-like [Oryzias javanicus] | 0.444 | 0.435 | 0.453 |
| iron-sulfur cluster assembly enzyme ISCU, mitochondrial-like isoform 1 [Oryzias javanicus] | 0.405 | 0.424 | 0.386 |
| LOW QUALITY PROTEIN: eukaryotic translation initiation factor 3 subunit H-like [Oryzias javanicus] | 2.747 | 2.855 | 2.644 |
| mammalian ependymin-related protein 1-like [Oryzias javanicus] | 0.489 | 0.527 | 0.454 |
| mitochondrial uncoupling protein 2-like isoform X1 [Oryzias javanicus] | 3.212 | 3.038 | 3.395 |
| nidogen-2-like [Oryzias javanicus] | 4.072 | 3.491 | 4.749 |
| nuclear distribution protein nudE-like 1-B-like [Oryzias javanicus] | 0.478 | 0.464 | 0.492 |
| pancreatic progenitor cell differentiation and proliferation factor A-like isoform 1 [Oryzias javanicus] | 0.216 | 0.203 | 0.229 |
| peptidyl-prolyl cis-trans isomerase FKBP2-like [Oryzias javanicus] | 0.407 | 0.469 | 0.353 |
| pirin-like [Oryzias javanicus] | 0.496 | 0.495 | 0.496 |
| plexin-D1-like [Oryzias javanicus] | 2.312 | 2.173 | 2.460 |
| probable ATP-dependent RNA helicase DDX5-like [Oryzias javanicus] | 0.406 | 0.406 | 0.406 |
| pyruvate dehydrogenase [lipoamide] kinase isozyme 2-like [Oryzias javanicus] | 2.168 | 1.985 | 2.368 |
| rab3 GTPase-activating protein non-catalytic subunit-like [Oryzias javanicus] | 3.656 | 3.269 | 4.088 |
| RNA-directed DNA polymerase from mobile element jockey-like [Oryzias javanicus] | 2.031 | 1.972 | 2.092 |
| SH3 domain and tetratricopeptide repeat-containing protein 2-like [Oryzias javanicus] | 2.207 | 1.882 | 2.589 |
| testican-2-like isoform 1 [Oryzias javanicus] | 0.383 | 0.427 | 0.344 |
| testis-expressed sequence 2 protein-like [Oryzias javanicus] | 0.386 | 0.404 | 0.369 |
| thyroid transcription factor 1-associated protein 26 homolog [Oryzias javanicus] | 0.491 | 0.435 | 0.554 |
| ubiquitin-conjugating enzyme E2 A-like isoform X1 [Oryzias javanicus] | 0.472 | 0.513 | 0.434 |
| 서열번호 | 유전자 | 실험1 | 실험2 | 실험3 |
| 1 | coiled-coil domain-containing protein 43-like isoform 1 [Oryzias javanicus] | 0.436 | 0.550 | 0.346 |
| 2 | ketohexokinase-like [Oryzias javanicus] | 0.464 | 0.473 | 0.455 |
| 3 | 14 kDa phosphohistidine phosphatase-like [Oryzias javanicus] | 0.459 | 0.494 | 0.426 |
| 4 | eukaryotic translation initiation factor 3 subunit C-like, partial [Oryzias javanicus] | 0.440 | 0.512 | 0.379 |
| 5 | testican-2-like isoform 1 [Oryzias javanicus] | 0.383 | 0.427 | 0.344 |
| 6 | ADP-ribosylation factor-like protein 5C-like [Oryzias javanicus] | 0.332 | 0.366 | 0.302 |
| 7 | KH domain-containing, RNA-binding, signal transduction-associated protein 1-like [Oryzias javanicus] | 0.401 | 0.472 | 0.340 |
| 8 | nucleoplasmin-like protein NO29-like [Oryzias javanicus] | 0.440 | 0.476 | 0.406 |
| 9 | choriogenin H precursor [Oryzias javanicus] | 0.415 | 0.349 | 0.495 |
| 10 | NEF1 protein [Oryzias javanicus] | 0.486 | 0.560 | 0.423 |
| 11 | dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48 kDa subunit-like [Oryzias javanicus] | 0.496 | 0.577 | 0.426 |
| 12 | signal peptidase complex subunit 3-like [Oryzias javanicus] | 0.478 | 0.576 | 0.396 |
| 13 | pleckstrin homology domain-containing family A member 6-like [Oryzias javanicus] | 0.466 | 0.391 | 0.557 |
| 14 | retinol dehydrogenase 12-like [Oryzias javanicus] | 0.457 | 0.540 | 0.387 |
| 15 | hepcidin-like [Oryzias javanicus] | 0.206 | 0.223 | 0.189 |
| 16 | interferon-induced protein 44-like [Oryzias javanicus] | 0.429 | 0.531 | 0.346 |
| 17 | ADP-ribosylation factor-like protein 5C-like [Oryzias javanicus] | 0.397 | 0.450 | 0.351 |
| 18 | dnaJ homolog subfamily C member 15-like isoform X1 [Oryzias javanicus] | 0.394 | 0.405 | 0.383 |
| 19 | beta-2-glycoprotein 1-like [Oryzias javanicus] | 0.479 | 0.439 | 0.523 |
| 20 | complement C1q-like protein 2-like [Oryzias javanicus] | 0.349 | 0.405 | 0.301 |
| 21 | sodium-coupled neutral amino acid transporter 2-like [Oryzias javanicus]] | 0.489 | 0.433 | 0.551 |
| 22 | zinc finger and BTB domain-containing protein 1-like [Oryzias javanicus] | 0.349 | 0.417 | 0.291 |
| 23 | actin, cytoplasmic-like [Oryzias javanicus] | 0.466 | 0.536 | 0.406 |
| 24 | complement C1q-like protein 2-like [Oryzias javanicus] | 0.200 | 0.167 | 0.240 |
| 25 | fatty acid desaturase 2-like [Oryzias javanicus] | 0.449 | 0.391 | 0.515 |
| 26 | nuclear distribution protein nudE-like 1-B-like [Oryzias javanicus] | 0.415 | 0.383 | 0.450 |
| 27 | actin, alpha, cardiac muscle 1a [Oryzias javanicus] | 0.453 | 0.519 | 0.395 |
| 28 | multiple coagulation factor deficiency protein 2-like [Oryzias javanicus] | 0.447 | 0.469 | 0.426 |
| 29 | uridine phosphorylase 2-like [Oryzias javanicus] | 0.412 | 0.428 | 0.396 |
| 30 | coiled-coil domain-containing protein 109B-like, partial [Oryzias javanicus] | 0.468 | 0.475 | 0.461 |
| 31 | DNA damage-inducible transcript 4 protein-like [Oryzias javanicus] | 0.274 | 0.311 | 0.241 |
| 32 | pirin-like [Oryzias javanicus] | 0.478 | 0.522 | 0.439 |
| 33 | ubiquitin-conjugating enzyme E2 A-like isoform X1 [Oryzias javanicus] | 0.472 | 0.513 | 0.434 |
| 34 | coagulation factor XI-like [Oryzias javanicus] | 0.476 | 0.425 | 0.533 |
| 35 | palmitoyl-protein thioesterase 1-like [Oryzias javanicus] | 0.305 | 0.316 | 0.295 |
| 36 | 39S ribosomal protein L12, mitochondrial-like [Oryzias javanicus] | 0.443 | 0.401 | 0.490 |
| 37 | thyroid transcription factor 1-associated protein 26 homolog [Oryzias javanicus] | 0.472 | 0.408 | 0.547 |
| 38 | spermidine synthase-like [Oryzias javanicus] | 0.482 | 0.565 | 0.411 |
| 39 | AN1-type zinc finger protein 5-like isoform 1 [Oryzias javanicus] | 0.225 | 0.240 | 0.212 |
| 40 | glycogen debranching enzyme-like [Oryzias javanicus] | 0.485 | 0.540 | 0.435 |
| 41 | phosphoglycerate mutase 1-like [Oryzias javanicus] | 0.285 | 0.253 | 0.321 |
| 42 | lysyl oxidase homolog 2A-like [Oryzias javanicus] | 0.395 | 0.469 | 0.332 |
| 43 | probable ATP-dependent RNA helicase DDX5-like [Oryzias javanicus] | 0.483 | 0.484 | 0.483 |
| 44 | testis-expressed sequence 2 protein-like [Oryzias javanicus] | 0.386 | 0.404 | 0.369 |
| 45 | anti-apoptotic protein NR13-like [Oryzias javanicus] | 0.434 | 0.506 | 0.372 |
| 46 | sodium-dependent phosphate transporter 1-B-like [Oryzias javanicus] | 0.433 | 0.445 | 0.422 |
| 47 | peptidyl-prolyl cis-trans isomerase FKBP2-like [Oryzias javanicus] | 0.445 | 0.460 | 0.430 |
| 48 | beclin 1 [Oryzias javanicus] | 0.347 | 0.317 | 0.379 |
| 49 | methyl-CpG-binding domain protein 3-like [Oryzias javanicus] | 0.435 | 0.350 | 0.540 |
| 50 | heterogeneous nuclear ribonucleoprotein A/B-like [Oryzias javanicus] | 0.394 | 0.353 | 0.441 |
| 51 | inositol oxygenase-like [Oryzias javanicus] | 0.240 | 0.199 | 0.289 |
| 52 | 15-hydroxyprostaglandin dehydrogenase [NAD(+)]-like [Oryzias javanicus] | 0.494 | 0.461 | 0.529 |
| 53 | pancreatic progenitor cell differentiation and proliferation factor A-like isoform 1 [Oryzias javanicus] | 0.240 | 0.258 | 0.223 |
| 54 | cytochrome P450 2D10-like isoform 1 [Oryzias javanicus] | 0.422 | 0.483 | 0.369 |
| 55 | mammalian ependymin-related protein 1-like [Oryzias javanicus] | 0.489 | 0.527 | 0.454 |
| 56 | leucine--tRNA ligase, cytoplasmic-like [Oryzias javanicus] | 0.391 | 0.480 | 0.319 |
| 57 | iron-sulfur cluster assembly enzyme ISCU, mitochondrial-like isoform 1 [Oryzias javanicus] | 0.467 | 0.517 | 0.422 |
| 58 | ras association domain-containing protein 7-like [Oryzias javanicus] | 0.494 | 0.538 | 0.454 |
| 서열번호 | 유전자 | 실험1 | 실험2 | 실험3 |
| 59 | insulin-induced gene 1 protein-like [Oryzias javanicus] | 3.112 | 3.558 | 2.723 |
| 60 | putative hexokinase HKDC1-like [Oryzias javanicus] | 5.354 | 4.448 | 6.445 |
| 61 | fibulin-2-like [Oryzias javanicus] | 11.492 | 9.978 | 13.236 |
| 62 | nidogen-2-like [Oryzias javanicus] | 4.993 | 4.081 | 6.110 |
| 63 | motile sperm domain-containing protein 1-like isoform X1 [Oryzias javanicus] | 2.331 | 2.266 | 2.397 |
| 64 | SH3 domain and tetratricopeptide repeat-containing protein 2-like [Oryzias javanicus] | 2.258 | 2.548 | 2.002 |
| 65 | ankyrin-1-like [Oryzias javanicus] | 2.315 | 2.593 | 2.067 |
| 66 | Ubiquitin-like protein [Oryzias javanicus] | 4.160 | 5.240 | 3.302 |
| 67 | myb-binding protein 1A-like protein-like [Oryzias javanicus] | 8.172 | 9.956 | 6.708 |
| 68 | beta-galactoside-binding lectin-like [Oryzias javanicus] | 10.424 | 12.100 | 8.980 |
| 69 | microfibril-associated glycoprotein 4-like [Oryzias javanicus] | 27.448 | 24.386 | 30.895 |
| 70 | Na/K ATPase alpha subunit isoform 1b [Oryzias javanicus] | 2.199 | 2.044 | 2.366 |
| 71 | transmembrane protein 230-like [Oryzias javanicus] | 2.113 | 2.485 | 1.796 |
| 72 | rab3 GTPase-activating protein non-catalytic subunit-like [Oryzias javanicus] | 4.011 | 3.553 | 4.528 |
| 73 | 40S ribosomal protein S16-like isoform X1 [Oryzias javanicus] | 2.039 | 2.341 | 1.775 |
| 74 | LOW QUALITY PROTEIN: selenoprotein H-like [Oryzias javanicus] | 2.046 | 2.220 | 1.885 |
| 75 | GTP:AMP phosphotransferase, mitochondrial-like [Oryzias javanicus] | 2.539 | 2.543 | 2.535 |
| 76 | NEDD8-activating enzyme E1 catalytic subunit-like isoform X1 [Oryzias javanicus] | 3.141 | 3.104 | 3.178 |
| 77 | ubiquitin-like protein-like [Oryzias javanicus] | 2.567 | 2.554 | 2.580 |
| 78 | fructose-bisphosphate aldolase B [Oryzias javanicus] | 2.111 | 2.230 | 1.997 |
| 79 | chaperone activity of bc1 complex-like, mitochondrial-like [Oryzias javanicus] | 2.033 | 2.003 | 2.063 |
| 80 | transcription factor AP-2-alpha-like [Oryzias javanicus] | 2.621 | 2.248 | 3.055 |
| 81 | cysteine-rich protein 1 (intestinal) [Oryzias javanicus] | 2.522 | 2.611 | 2.436 |
| 82 | apolipoprotein A-IV-like [Oryzias javanicus] | 6.726 | 6.378 | 7.092 |
| 83 | microfibril-associated glycoprotein 4-like [Oryzias javanicus] | 4.864 | 6.519 | 3.630 |
| 84 | mitochondrial uncoupling protein 3 [Oryzias javanicus] | 2.538 | 2.720 | 2.368 |
| 85 | h-2 class II histocompatibility antigen, I-A beta chain-like [Oryzias javanicus] | 3.961 | 3.727 | 4.210 |
| 86 | hemoglobin subunit beta-like [Oryzias javanicus] | 2.245 | 2.626 | 1.919 |
| 87 | Mhc class I A precursor [Oryzias javanicus] | 2.125 | 2.549 | 1.771 |
| 88 | dehydrogenase/reductase SDR family member 11-like [Oryzias javanicus] | 2.366 | 2.131 | 2.628 |
| 89 | fructose-bisphosphate aldolase B-like isoform X2 [Oryzias javanicus] | 2.249 | 2.120 | 2.386 |
| 90 | hemoglobin subunit beta-like [Oryzias javanicus] | 2.043 | 1.990 | 2.097 |
| 91 | fatty acid binding protein 7, brain, a [Oryzias javanicus] | 35.896 | 49.406 | 26.080 |
| 92 | fructose-bisphosphate aldolase B-like [Oryzias javanicus] | 2.243 | 1.869 | 2.693 |
| 93 | microfibril-associated glycoprotein 4-like [Oryzias javanicus] | 11.008 | 13.200 | 9.180 |
| 94 | brain-type fatty acid binding protein [Oryzias javanicus] | 35.021 | 55.907 | 21.937 |
| 95 | deleted in malignant brain tumors 1 protein-like [Oryzias javanicus] | 18.175 | 18.332 | 18.019 |
| 96 | acidic mammalian chitinase-like [Oryzias javanicus] | 41.929 | 41.112 | 42.762 |
| 97 | myelin proteolipid protein-like isoform 1 [Oryzias javanicus] | 2.243 | 2.102 | 2.394 |
| 98 | sphingomyelin phosphodiesterase 2-like [Oryzias javanicus] | 2.422 | 2.069 | 2.836 |
| 99 | 1,4-alpha-glucan-branching enzyme-like [Oryzias javanicus] | 2.017 | 1.706 | 2.384 |
| 100 | GTP cyclohydrolase 1-like [Oryzias javanicus] | 2.179 | 2.169 | 2.188 |
| 101 | creatine kinase B-type-like [Oryzias javanicus] | 2.496 | 2.927 | 2.129 |
| 102 | c-C motif chemokine 20-like [Oryzias javanicus] | 2.003 | 1.942 | 2.065 |
| 103 | pleiotrophin-like [Oryzias javanicus] | 2.555 | 2.531 | 2.578 |
| 104 | LOW QUALITY PROTEIN: eukaryotic translation initiation factor 3 subunit H-like [Oryzias javanicus] | 2.747 | 2.855 | 2.644 |
| 105 | cadherin-11-like, partial [Oryzias javanicus] | 2.213 | 1.897 | 2.580 |
| 106 | plexin-D1-like [Oryzias javanicus] | 2.312 | 2.173 | 2.460 |
| 107 | translation initiation factor eIF-2B subunit beta-like [Oryzias javanicus] | 2.049 | 1.840 | 2.281 |
| 108 | cholinephosphotransferase 1-like [Oryzias javanicus] | 2.179 | 1.859 | 2.556 |
| 109 | Guanine nucleotide-binding protein GI/GS/GO subunit gamma-2 [Oryzias javanicus] | 2.146 | 1.982 | 2.324 |
| 110 | 5-aminolevulinate synthase, nonspecific, mitochondrial-like [Oryzias javanicus] | 2.236 | 2.712 | 1.843 |
| 111 | kinesin heavy chain isoform 5C-like [Oryzias javanicus] | 2.168 | 2.635 | 1.784 |
| 112 | pyruvate dehydrogenase [lipoamide] kinase isozyme 2-like [Oryzias javanicus] | 2.053 | 1.919 | 2.197 |
| 113 | apolipoprotein Eb-like [Oryzias javanicus] | 59.765 | 51.494 | 69.366 |
| 114 | caspase-6-like [Oryzias javanicus] | 4.450 | 3.843 | 5.152 |
| 115 | apolipoprotein C-II-like isoform X1 [Oryzias javanicus] | 2.804 | 2.570 | 3.058 |
| 116 | sodium- and chloride-dependent taurine transporter-like [Oryzias javanicus] | 2.012 | 1.755 | 2.305 |
| 117 | mitochondrial uncoupling protein 2-like isoform X1 [Oryzias javanicus] | 2.452 | 2.506 | 2.400 |
| 118 | 5-aminolevulinate synthase, erythroid-specific, mitochondrial isoform 1 [Oryzias javanicus] | 2.892 | 3.547 | 2.358 |
| 119 | FYVE, RhoGEF and PH domain-containing protein 3-like [Oryzias javanicus] | 2.101 | 1.759 | 2.509 |
| 120 | RNA-directed DNA polymerase from mobile element jockey-like [Oryzias javanicus] | 2.031 | 1.972 | 2.092 |
| 121 | kelch-like protein 12-like isoform X3 [Oryzias javanicus] | 2.023 | 2.030 | 2.016 |
| 122 | retinol dehydrogenase 7-like [Oryzias javanicus] | 2.955 | 3.102 | 2.815 |
| 123 | heme oxygenase-like [Oryzias javanicus] | 2.126 | 2.273 | 1.989 |
| 124 | carnitine O-acetyltransferase-like [Oryzias javanicus] | 2.049 | 2.166 | 1.938 |
| 125 | apolipoprotein A-IV-like [Oryzias javanicus] | 2.053 | 1.880 | 2.242 |
| 126 | serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit delta isoform-like [Oryzias javanicus] | 2.012 | 2.253 | 1.796 |
본 발명에서 제시한 탄화플루오르옥탄술폰산염(Perfluorooctane sulfonate; PFOS) 노출에 대응하는 바다송사리(Oryzias javanicus)의 유전자는 수생태계의 내분비계장애물질 오염 및 이에 따른 수생태계의 상황을 모니터링하고 진단하기 위한 바이오센서의 성분으로 유용하게 사용될 수 있다. 아울러, 제시된 유전자들의 기능에 의거하여 본 생물의 대사/생리변화를 구체화함으로써 앞으로 일어날 수도 있는 병리적 현상을 예측할 수 있는 생체지표 및 센서로 이용할 수 있으며, 수생태계의 스트레스원 검출 또는 건강 진단방법에 효과적으로 이용될 수 있다.
<110> Korea Institute of Ocean Science and Technology
<120> Perfluorooctane sulfonate responsive genes in Oryzias javanicus
and the method for diagnosing aquatic environment pollution using
the same
<130> 2016P-01-009
<160> 126
<170> KoPatentIn 3.0
<210> 1
<211> 702
<212> DNA
<213> Oryzias javanicus
<400> 1
aagcagtggt atcaacgcag agtggccatt acggccgggg acagcgccaa gatggcggcg 60
cccttgtcag acgccgggga gtttgaaatc tggttaaatg agcgattaga taatctggaa 120
gtggatcgag aggtgtacgg ggcttacatt ttggggattc tacaagaaga agaaaatgac 180
gaggagaagg aagacgctct tcgcgggatt ttatcggcgt ttctggaaga ggatgccatg 240
gaagatgtct gcaaacagat catccagcag tgggcagact attccagccg atcagcagcc 300
atgaaaaatg cagatgatgc tgaggtccag gccatcgcca gcatgataga aaagcaagcg 360
cagatcgtgg tgaaacagaa ggaggtctct gaggagtcaa agaagaggaa ggaggctcta 420
ctggctcaat atgccaacat aacagacgag gaggatgaag ctgaagaaga ggagcctgca 480
ggtggaaacg acatcgctgc aaatgaaaga tccctgttca agaacaccaa cgtggaggag 540
gtgctccaca gacagaagca gaagcgggaa caggcccggg aagacgctca gaagaagaaa 600
gaaacggaca agatgcagcg agagaaggac aagctggcca agcaggacag gaaggagaag 660
gagaagaagc gcacacagaa aaatgaacgg cgaagataaa ac 702
<210> 2
<211> 689
<212> DNA
<213> Oryzias javanicus
<400> 2
agggaagaaa tgcagaggag caggtgaaaa tgatccagca ggtggagcgg tataacagca 60
cgctgcctca gcaggagagg atcaccgtgt ctgtggagat cgagaaaatc cgtgagccgc 120
tctatcagct gttcccatac ggcgatgtgg tgtttgtcag taaagacgtc gcacgtcact 180
ttggcttcga gtccgctgaa gctgctgtga gaggatttta caatcgagtc aaaaaagggg 240
ccgtccttat ctgtgcctgg gcagagaaag gagccgatgc tttgggtcct gacggcgtgc 300
tcgttcattc agacgcgttt cctcctgaga aactggtgga cactctcgga gctggagaca 360
cctttaatgc tggagtcatc ttcatgctgt caaacggaaa aagtttgcag gaagctctca 420
actttggctg caagattgca gggaggaagt gcggtttcca tggttacgat ggcatcgttg 480
agaagacaaa cacctgaaga acaagacatg tttttacaga atttatttca ggttaatatt 540
gttttctatt ttgttgccat ggagtaaatt tttatgatta ttgtaaataa agaagcactt 600
agaagccttt ttcttcatca aaattttatc ttcaatcatt gttttaatgc aagtattgaa 660
aaatgtaaat aaatgtaact tcctttaag 689
<210> 3
<211> 640
<212> DNA
<213> Oryzias javanicus
<400> 3
aagcagtggt atcaacgcag agtgccatta cggccggggg ctgtccggca gagatcagac 60
gcgcgcgcgt gtcgctttaa ggcaggaaat agaatccgag cgtccaaata aaaccgattc 120
tactgctgcg gccgtcgggg tgcagctgtc gttataatgg cggagtgtct ggccaaagtc 180
cccgaagtgg agatcgatcc cgacggaacc tttaaataca tcctgctcag agtcaaagtg 240
aaagatggcg aagcgcataa agatgtggtc cgaggcacga aaagtgcaga ataccacaat 300
catatttttg agaaggtgag tccggctctg gagtgtctgg ggatggagtg taaatgtctc 360
ggcggaggga aaatagagca caacagccag gagaagaaaa tccgagtgtt tggagaatcc 420
actgcctttg gcaaagccga ccacgccgtc tccgtggaga agctgaagtc tgtcttcagc 480
gactatgaca tcacctggtc tgatgacaca aaatgaacat tttttttccc cccaaactct 540
cacatttaaa tcatattcac catgaataaa actgaatgtt gacaaaaaaa anaaaaaaaa 600
aaaaggccgc ctcggccact ctgcgttgat accactgctt 640
<210> 4
<211> 617
<212> DNA
<213> Oryzias javanicus
<400> 4
aagcagtggt atcaacgcag agtgccatta cggccggggc taacccggca agaccgagct 60
cgcggcattc gcacgttttg tgacgaccgc acagcaaaac tggatttcaa ggccgttgaa 120
gttgtgaaag cgacgcacat tgtaccttac atttgcacat agattgacct cacccttcag 180
cgatgtctcg tttctttgcc accgggtccg acagtgaaag tgaggagtcc tcatccgccg 240
atgagatcac ccccaaagca cccgggacta ctttgaagca atccttgctg cttagtgatg 300
atgaggagga caccaaaagg gtggtgcgca gcgccaagga taagaggttt gaggagttga 360
ccaacctgat taagacgatt cgcaacgcca tgaagatccg cgacatggcc aaatgtttgg 420
aggagtttga gcagctgtgc cgagccttcc ttaaaagcaa gaacatagtg gataaagaag 480
gcgttccgtc tttttattat ccgtcttctg gctgatctgg aggattatct gaaccagctt 540
tgggaggaca aagagggcaa gaaaaaaaaa aaaaaaaaaa aggccgcctc ggccactctg 600
cgttgatacc actgctt 617
<210> 5
<211> 1930
<212> DNA
<213> Oryzias javanicus
<400> 5
cggggaattt catggaggac gagcagtggc tgtccaccat ttcacagtac agccgcaaga 60
tcaagcactg gaatcgcttc agagacgagg tcgaggatga ttatgttcgg acctgggatg 120
aaaatcaaag ctccaacgac aacgtagaca ccaccaagga cccgtgtcaa aaggttaagt 180
gcagccgaca caaggtgtgt attgcccagg gctaccagag agctgtgtgc gtcaatcgta 240
agaagctgga acacagactg aagcagcccg ccctccgctc tcctgatgga aactgtcagc 300
cctgtcccat ctcctccacc gggcccgtgt gcggatcgga cggtcacaac tacgcttcga 360
agtgcaagct ggagcagcag gcgtgcctta cagggaagga tctcaccctg aaatgttcag 420
gtctgtgtcc ttgtccaact gcagctccta cgtccaagga aggcaagcgt gagggctgca 480
ccggacagga cctgtctgat ctcggcgagc gtcttaggga ttggttccag cttctgcaga 540
gcaatgctaa gcagaacaac agtaagcctg gagccagatc cagcaccaca tcagtgctgg 600
acaggagttt ggtggccagc tgtaaggact ccataggttg gatgttctcc aagctggaca 660
ccaataatga cctgtatttg gatcaggccg agctggccgc catcaacctg gacaagtacg 720
agatctgcat tcgtcccttt ttcaactcct gcgactcgta cagggatggc aaggtctcca 780
cggcggagtg gtgcctctgc ttctggcgag agaagccacc ctgcttggct gaactggaga 840
ggatccaagt cttggatggt ggaaagagaa agtttggtcg attcatcccc agctgcaatg 900
aagacggctt cttcaggagg cagcagtgtg acaggggcga gtgctggtgc gtggatcaga 960
acggaggaga aatagctgga tccaggattc ggggaaagcc aaactgtgat tatgaaatgg 1020
catattcagg agatttcggc agcggggtcg gatgggagga tgaggaggag aaagaggcag 1080
aggagactgc agaggaggct gaagaggagg agggagaagt gggagaggtg gatgacgggg 1140
gatatatttg gtaaatgccc aacgtgaaag ccaacctctc tacaggagca cttccatggt 1200
cctgaaaata cacacacaca cgcaccaaca aacacacaga cagatggaca cgatgaaccg 1260
ctcacactca gaacaacact tgctttggaa accgtgaggg agaggagtta aataagctgg 1320
gaggttgaag atgggagcat ttaatgttct taaaacgttc aagggaaggg gagatttatt 1380
gctattaacc cttcgtgggt ctggagttga gatttttgtc cttcgtacgt gtgactcctt 1440
gttcttcttt gttgtatgtc ttttgaacta tctttgagca gtttttagct gatgcataat 1500
ggaggcttta agaagctgca ggtgctcgtc atgtgcttgc gttgaaaaaa actgagctcg 1560
tctgggtgca tttgggggct tttctctgcg gtcggaaagc acgtcggaca ggaagccggg 1620
tcagaaaaca gacaaacacc ctttgacttt atgatttcca tgtcatgcgc tttggctcat 1680
atgtgacatt agtgctgtct ccctaatgac ttattaagaa caagtttctc acagatcact 1740
cttaagcttg tatataatgt gtgtatccga aggcagaatg tcctcttcta gtcaaatgcc 1800
ttgaaagaac agcagattga agctgcttag ctgacaagtg tgcatcatgt cataatgtgg 1860
attaattact gtgtttaata aaaaaaaaga attaaataaa ggtatctctg tggctcagct 1920
ggccaccatt 1930
<210> 6
<211> 292
<212> DNA
<213> Oryzias javanicus
<400> 6
aacacaaagt catcgttgtg ggtttggaca acgccggaaa gacaaccatc ctttaccagt 60
ttctaacgaa agaagccgtc cacacgtcgc ccaccattgg cagcaacgtt gaggagatca 120
ttgttcggaa cacgcacttc atggtttggg acattggagg gcaggagagt ctgagagcca 180
gctggtactc ctactacagc aacacagaga tcatcctttt ggtggtggac agcactgacc 240
gtgaaaggct gcctataacc cgaggggagc tccaccaaat gcttgcacac ga 292
<210> 7
<211> 552
<212> DNA
<213> Oryzias javanicus
<400> 7
acttttgaaa gggtaagtga gttacaacag aggcaattgc cttagcaacc ctctaccagt 60
agtcgttcaa tatcttccat atggatgttc tctataggag cccttagact gccgcgatgg 120
gggggctttg cctccagcac cagagcttga ccacgagttg tcccaatcat cttgagcata 180
ggagtcgtat cctgtgtcct gggcttcacc gtgcccatag tcataatact cagtatctgc 240
agaagcagat tgctggctgt aataactatc atatccctca taagcaggct cggcgtaacc 300
ctcctcatat gaaggatatt catcataacc gtcgggttta gcttgtgacc ctgaagggcc 360
accctggtgt tgctggtgag acatggcggc agatgggagc atcctcgcgg cgcctgcagc 420
aggtggtcta gagcgcgcag aagtgccgcc tcgagtagga gcagaaggaa gtccccccct 480
gcctgctggt gctcctcgag gggcagtgcc gcgtccgagt cctccacgcg gtgctccgcc 540
acgcacagcc cc 552
<210> 8
<211> 526
<212> DNA
<213> Oryzias javanicus
<400> 8
acccacgtcg ttgttcgtgc tggttctagt tggttacaaa aaaaatccta aggaaaactt 60
atttagcttg acaaaatgtc tttcagcgac gacgaatcta ttgattttaa atcagccggg 120
ccatcgaagc tggaaagcta cttgttcagc tgtgaattat catccaaagt accttcctat 180
agtttccacg gtgatgaaga ggaggatttg gaacattttc ttgagcttag aacagtttgc 240
ctcggagaag gtgccaagga ggagagcaat gtcgtggaag taacggctat gaaccacgag 300
ggaaagaaca tttcagtgcc catcgtcagt cttcacttaa agtgccttcc aatgattagt 360
ctgggagaat ttgaactcaa agccccggtg accatcagtc tcaaagctgg atcaggacct 420
gttgttgtca gtggtcttca ccttattgct tcacaggagg aagactctga catgtctgag 480
gaggaagaaa cagaagagga agatgaggag gaagaggaag aagaag 526
<210> 9
<211> 491
<212> DNA
<213> Oryzias javanicus
<400> 9
ccgcagtggg acattttgat tgacggatgt ccatatgaag atgatcgcta cctgtcggca 60
ttggttccaa ttgatgcctc ttctggattg ccgttcccaa ctcatcacag acgcttccta 120
ttcaagatgt ttacctttgt tgatcctcat tcaatggaac cactaaggga aaaggtgtac 180
attcactgta gcacagctgc atgcgttcca ggacagggta ccagttgtga accctcatgc 240
agcagaagaa aaggaagaga tactgacgct gtatccatta gaacggatga aagaaaggtt 300
gtggtatcgt ctggagaagt gctcatggtc gccgaagctg cacagcctga actgtgaaac 360
gacagaagct ccagagttct gaaaaaataa cattatgaaa atctgtttca tcatgggtca 420
aaattaaatg cataaaataa naaaaaaaaa aaaaaggccg cctcggccac tctgcgttga 480
taccactgct t 491
<210> 10
<211> 427
<212> DNA
<213> Oryzias javanicus
<400> 10
tttttctgta caaatccacc tttacttgag tcaatatgat ccaaagaaaa acctgtcagt 60
gccctcttat taaatccaga atcctgaaaa acacgagtga agtcctcgat tctgctggaa 120
tgtgtgtctt gaagacagtt atcttcaaac atagatgccg acccagagta gcaggaagag 180
cacgccaaag cccatgaaaa gtgaagccac aagggagatc agcagctcct tatagatgtc 240
cctcgtgtat tttgttgatg taacctcata tacgaagaac caggcggtga agaatgtgcc 300
aatggccaac aggaccacgg tgaggtgggg gaagacggcc gggttcaccg ggctggtgta 360
tctggtcata gcctcgagtt ccatttcttt tgaatgaagc gaacctcttg ctgatgtcga 420
ctatggt 427
<210> 11
<211> 1790
<212> DNA
<213> Oryzias javanicus
<400> 11
aagcagtggt atcaacgcag agtggccatt acggccgggg acgtcgcttt ctgctccaag 60
atggcggcgc tgacagcagt cacacagacc cggtccggtt ctatgttgac ccagaaacgg 120
atccgcacga tggcggagag ccgcttcggt tccgtcagcc caaagctcgt gctgctgttc 180
tggttggcgt ccatgctgcc gcgcgctgag gctgacggga aaacgctggt gctgctggac 240
aacctgaaca tcagagacac tcattcggtc ttcttccgca gtctggccga tcgaggcttt 300
gacctgacct ttaagaccgc ggacgacccc tccctgtctt tgatcaaata cggacagttc 360
ctgtacgatc acctgatcct gttctctcca tcagtggaag attttggtgg aagcattaac 420
gtggagacca tcacatcttt catcgatggg gggggcaatg ttctggtggc tgccagctca 480
gacatcggtg accctctgcg ggagctgggc agcgagtgcg gcatcgagtt cgatgaggag 540
aagacggctg tcatcgacca tcacaactac gacgtgtcgg atcctggaga gcacaccctg 600
atcgtggctg accctcagaa catgctgaag gcccccacca tcgtcgggaa gcctacagat 660
aaacccgtct tatttaaggg tgtgggcatg gtggccgacc cggacaaccc gctggtcctt 720
gacatcctca ctggctcttc cacttcctac tcctacttcc ctgacagagc catctcccag 780
tacccccacg ccgtgggtaa gaacacgctg ctgatcgccg gccttcaggc ccggaacaac 840
gcccgggtgg ttttcagtgg ctccctggac ttcttcagtg acgccttctt caactccgcg 900
gtgcagaaag ccacgccggg ttctccgagg cacgagcaga cggggaacat ggagctggct 960
gtggctctgt cccgctgggt gttcaaagag gcgggggtcc tccgagtggg agctgtgagc 1020
caccatcgcg ttggagaggc cacgcccccc accgcataca ccatcacaga ccttgtggag 1080
tacagcatcg tcattgaaat gctgtccgag ggccgctggg tcccttttga cggggacgac 1140
atccagctgg agttcgtgag gatcgacccg ttcgtcagaa ctttcctcca gaaaaacgga 1200
ggtaaataca gcgtccagtt caagctgccc gacgtctacg gagtcttcca gttcaaagtg 1260
gattacaacc ggctgggata cacccacctg tactcctcca cccaggtgtc ggtgcgcccc 1320
ctgcagcaca ctcagtacga gcgcttcatc ccctcggcct tcccgtacta cgccagtgcc 1380
ttctccatga tggccggact cttcgtcttc agcgtggttt tcctgcacat gaaggagaaa 1440
gagaagacgg actagatcag aggaaccgca gagcaaagag aacttgtgtg aagagttcaa 1500
agtgaatgtc tgtgagtctg tgggacaggg acttggactt gaatttttat ttatctggca 1560
ctcgtttgga atctctgggg aaaggggggt ttactgactt tgtctacttt ttttaagcta 1620
cgtcacaatt agccagatgt tcattcaaaa gtcagaaatg ttgtttttgc atcagataaa 1680
ttccatttga agagagcaga aatgtcaggc actgaaaacc tttgttttga taaggattag 1740
tttccataaa aatctggggt ctgctaaata aaatgttgac attccactct 1790
<210> 12
<211> 423
<212> DNA
<213> Oryzias javanicus
<400> 12
gttggctttg gtagcgtatt cagctgacag ataaatgaac agctgcttca cgttccagtc 60
aaaaatgggt tgcagatcag ctgagaggtc aaaagtcaca aagccaaggt cgctccgttc 120
tcttggtcca gtaaaatcat cgacattctt cagcaggact ctggacacac ggatgtccac 180
cgggaccctc cgatccttga aggcggtggt gatgaagcag ccgaaggtga gcaccgccat 240
gacgctcagg gagaaggcga acagcgagtt ggctctggac aaaaccgtat tcatctttga 300
agcttccgaa aaccgaccag aaccgcacag aaacactccg ctagctacag cagctcacaa 360
atgaatcgaa aactcaagct gtgccccggc cgtaatggcc actctgcgtt gataccactg 420
ctt 423
<210> 13
<211> 1755
<212> DNA
<213> Oryzias javanicus
<400> 13
ggacagagtg agcttgttcg aagacacatt cagaaagagt tttggcgagt ccaggatgtc 60
cttgagggac tacacaaaaa ccattcatcc agaggcactg acacagccaa gcacagagtg 120
gccagcggtg cgtctggtcc tttagcacca atagtccagc cagtcccctg agctcagtaa 180
gcctaaccag tccactcagc cccttctccc cagtgcaagg ttgccaggcc tcccccacca 240
aacaactggg cccggagggc agggcaggtg aacagagcag tccgagagat gttcaggacc 300
aggttcagga caaacaatcc acaaacaaag ttggtatcgt tcctccgaga accaagtctc 360
caactgaaga agcccacaga aatgcagctg aagcgccgcg gcacatctcc aaggaccgcc 420
caaagagtgc agtgtttcct gcagagctga ggtccaagat gagcctagag gagcaaaatg 480
agcgaattcg tcgtaaccag agcagctcca tgagagacaa gaggcgaagc cttaatctgt 540
cggctggccc gcttccacct aacttcaaag tggtccgaag gcgagtaaca gctcatgaaa 600
ttgacattaa ggacctggag gcagcagttc gcgatgacaa ccaagagtct ccatgggagg 660
agattgctcg gctcaggcgg ttgcagaatg acggggtgta tgacagcaga gagatgacag 720
ctcaagacca acccatgatc cctgagggcc tcctggatcc agagaacgac actcctctga 780
gtccagagga gcagaaagag aagcagaaga agctggatcg gatcaagact ctaatagcca 840
aatcaaacct gcagaacgtg gttccggttc tggatggtcc agtggagggt ggagcttcag 900
ccagctcaca gcaccagcta ctggaacagg agaagcggat tgagattacg tgtgctctgg 960
ctgcggaggc ttctcgtcgt agtcgtcttc tgtccgccca gtgtgcctcc agccccccca 1020
cgtcaccgag cagcctggtt cctccccatc ctccagtgac ttctttaacc ctgcacacat 1080
cctgaaagtg taagacgcac agctttggca gcagttaagt tcgatggagc atctttctga 1140
ggattcacag gagcaacatc aaaggaggga gcatccaaag tcattggagg caaggcaagg 1200
gaatgcgccc ccaccccctg aggatctaac ccatgtagca caaaggcttc agacctcttc 1260
tcgccaactt gtgtcatcat ctttaattta gctcaggaca aattcttctg aacctgctga 1320
cactgaaatc ttttacaata acatcgtcaa actgtgagct tactgtcagc tgcttttgac 1380
atgctctgaa acgttgggag gtgcgactcc taaggcttac tgtggagggg gagccccaac 1440
tgtttacttc tgtgtttatg tcggagagga aacttaagct tcaacgtccg cgtgcctgaa 1500
agtgtggaga aagaaacctc cacaaaaaaa agcaaaacaa aaaaaccctg ctgaacaatg 1560
taacacggtg gggcagatca ctgcctctga tattgtcttt gaaacagcac gccccaaatt 1620
aagaaaatca cactggagtg agcaggagcc ttcctgacat ctgtggattt cactgaagct 1680
ctgcagaact ggactggagg gtctcagatc ctctgcaggg ctggtccagc agctgactca 1740
gatcgtgcag tttct 1755
<210> 14
<211> 368
<212> DNA
<213> Oryzias javanicus
<400> 14
gatgaaccaa aactcaacat cctcatcaac aacgctggcg tgatggtttg tccgtacggg 60
aaaacggccg acggcttcga gatgcagatc ggtgtcaatc atctgggtca cttcctgttg 120
acctacttat tgatagacct gctgaaacgg tcggcgccgg cccgcatcgt caccgtgtcg 180
tccatggctc acgcctgggg ctccatcaac ctggaggacc tgaacagcga gaagagctac 240
agcaagagca aagcctacgc ccagagcaag ctggccaacg tcctcttcac gcgctcgctg 300
gccaaacgcc tggaaggtac cggcgtgacg gcgtactccc tccaccccgg cgtggtccag 360
accgactt 368
<210> 15
<211> 353
<212> DNA
<213> Oryzias javanicus
<400> 15
aaggcattca gcattgcagt tgcagtgaca ctcgtgctcg cctttatttg catccaggag 60
agctctgcca tcccagtcaa tgggcaggtc acagagctgg aagaagcagc aagcaatgac 120
actccagttg cggcacgtca cgagatgtca atgcaatcgt ggatgatgcc aaaccacatc 180
agggaaaagc ggcagagcca cctctccatg tgcagcgtgt gctgcaactg ctgcaagaac 240
tacaagggct gcggtttctg ctgcaggttc tgaggggtcc tgcgacaacc accacacatt 300
aatttattat taggcttgtt tttccatact ttccacaaga aattcacttt ttt 353
<210> 16
<211> 308
<212> DNA
<213> Oryzias javanicus
<400> 16
tctgactttt ccaatgactg aattatgtga ccagaataag cataatatga aacatcctaa 60
tgcaatggaa atgtacttgt attttattct ttcatatttt tctttgtcag taatctgtag 120
ttttcttcct ctcagtttag cttggagcag ccagtatcat cagaaactgt tggctatgtg 180
aagacaccga acctcgcaga aaagattcac tgtgtggttt ttgtggtgga cgcttccaaa 240
gttgcaactt accccaaaag cctcggcgcc acattcagga ggctcagaga acaaatcagt 300
gatctggg 308
<210> 17
<211> 556
<212> DNA
<213> Oryzias javanicus
<400> 17
ggatctacag aatgcagcgg tgctcattct agcaaataaa caagacataa agggctccat 60
gacagtagca gagatctccc agtgcctcac actcaactct atcacttcac acccctggca 120
tgtccaggcc tgctgcgccc taacaggaga aggtctacct gcctgcctag attggatgaa 180
atcacaggtt gtggtaaact gatgccactt ccagtgcctg aaacttcctt gaaccgcgcc 240
aaccttcgca cttttttgtg tgtttcaaag tgtttactgt gtgtggcatc tgtaacagag 300
actatagtct ccacgccttc tgcgtgtttg tgtgggagta ttcgaaacgg atggatgaac 360
attacagtga cctggacgtt ttatgttatt ttagtttgtt tatgcagttc tctttcttgt 420
aactgtggaa acacgcacta ctaggaactg tttttgttcc gtactgtgtg aagaggtgct 480
ttcagactgg atgccctacc tgacaagtca cattccactt tgcgtcattt tgcacgggtt 540
tggtacaaaa caaagg 556
<210> 18
<211> 280
<212> DNA
<213> Oryzias javanicus
<400> 18
aagcagtggt atcaacgcag agtggccatt acggccgggg atgaaaaaca tggcgaacac 60
agcagggacc agcatggatg actcgatgag atacgccgaa tattccccta aaacgaccac 120
aaggagcgac gcagaaatag acagaaagct gggagggact ctgattgcag ttggactctg 180
tgctgctgct gctggttttg caggtcgtta cgccttccag ctgtggaagc ctctgggaca 240
ggtcttctct gaaactgtaa agaagatgcc ctcatcgatt 280
<210> 19
<211> 264
<212> DNA
<213> Oryzias javanicus
<400> 19
ttcagcactt taatacattt tatatccttg gatcacaaca atctcatttt cattgtcacc 60
gtaaatgaaa gaaatgcatt ctggcacacc acatgttctt ctgtgagctc atgtatgtct 120
tgttgtcctt gcaggaggtg aagcagcaca catgctgatt tctgatggca gggtcttaga 180
ctttatgtta tactcaactt ttccaggttc ttcaaagcat gcagggattg ggagggttcc 240
atcgttgcag gtgctagaca cggg 264
<210> 20
<211> 247
<212> DNA
<213> Oryzias javanicus
<400> 20
tggatataac ccagagacag gcatcttcac ggctccagtc aaagggctgt actacttcag 60
ggtcactggg attgctggga gcaatgggga attaaacgca ggactcaaaa agaacggaca 120
gaacatgttt gcaatctacc agaaggcagg aacacaggct ggcacctcaa acgggatggc 180
tctggaactg gagcagggtg accgggtcta cgtccagatt tggccaaact tgaccgtggc 240
tgaccag 247
<210> 21
<211> 199
<212> DNA
<213> Oryzias javanicus
<400> 21
tctgaagcca aaaaagaaaa atgcccaaca agaagcagcc aggcgcagag atgaagtgcc 60
tcagcgcccc ccaggaggac gacagcagca ccaacagcag cgagttcccc taccccacct 120
ttgtcaagaa agttcctctg gaggggccat acccagagtt tgaccccgag tgtcagaact 180
tcctgccgga gcacagcac 199
<210> 22
<211> 164
<212> DNA
<213> Oryzias javanicus
<400> 22
caatggggaa ttaaacgcag gactcaaaaa gaacggacag aacatgtttg caatctacca 60
gaaggcagga acacaggctg gcacctcaaa cgggatggct ctggaactgg agctgggtga 120
ccgggtctac gtccagattt ggccaaactt gaccgtggcc gacc 164
<210> 23
<211> 122
<212> DNA
<213> Oryzias javanicus
<400> 23
aaaaggaaat cactgctctg gccccaccca ccatgaagat caagatcatt gcccctccag 60
agaggaagta ctctgtctgg atcggtggct ccatcctggc ttccctgtcc accttccagc 120
ag 122
<210> 24
<211> 120
<212> DNA
<213> Oryzias javanicus
<400> 24
aagcagtggt atcaacgcag agtggccatt acggccgggg acccaggaat ggaccacttt 60
ggggccgttt gaggcagaca aaacccttga attcaagaag gtcgtgacca acattggaaa 120
120
<210> 25
<211> 1539
<212> DNA
<213> Oryzias javanicus
<400> 25
aattcttgaa ggctaaaatg tgttatctga ttttatgcaa catagcaaag atctctcctt 60
ccaggtccat tttgtcattt atgaaggtat gcatcaagcc acagatcgcc tgactttttc 120
agagacctga caacatccac catgccgttc cacaaggatt tcatttcata gggaattcca 180
tgtttctcac acagttcacg gacctgtggc gccaccaagt ggtagttatg acgcggcatc 240
ctggggaaca agtggtgttc aatttggaag ttgaggtgtc ccgagaacca gtcattgaag 300
aaggactgtt caatattaca agtggacgac agctgcgagg tcacccagtc ttgtcgcttc 360
tcgtgctcca tctccatagg tatatggctc atttgagtta cccacacaaa ccagtgactc 420
tctaaaaatc tggcaaacat ctggagggca aaggaacccc agaagccata aagaggcacg 480
aagcagaaat agtagcggaa gtaatatgtc atggcccagg acaggtccac ccagtcacgg 540
cgggtaatca tggtgtaaaa tatattaaaa ttgtagaata ttgggattag tagaggtgga 600
cccacgagga agaaatactg gtgttggcga ttgtagggca ttttcttgat cttttttatt 660
ccgtactcta ctggttgagt gtttccaagt acgaagacgt ccatcacgtt aatatcaggg 720
tccttcctga agatattggg tttggcgtgg tgctggaaat gtctgtgatt ccaccaattg 780
gctgaagctc cctttaactg tccaatgaca aatttgtgta caatgtgatt ccatttagac 840
ttcttaaaga cagatagatg cccaaagtca tgctgcaacc accctgcctg tgcctgagca 900
attgccagca gtgtcgcaca caggaaagtc attgtccagc tggtccccca gtaaagaaca 960
gagagccatg cgagtgcctc cagcagtatg atgtgaccca gatggaggag gaaaacaaag 1020
gcttagcctt aaacaaacca tctttttctg cctggactcg aagggaatcg aaatctttta 1080
ttatggaatc attcttttct ctgtcctggc tgggctctgt tgctgccaac tctccaatgt 1140
ggagaggctt tagaaacttt tgtacgaagt cctgatctgg atgaaaggca gcaaatacct 1200
ctgtggcatc ctctccagca tagtggttga tgacacgaaa cccccctgga tgccttttgg 1260
cccaattagt gacgttgtaa acctttcgtt tgaccaccag ccactgatca ttcctgttgc 1320
tgtgcttctg cacctcctcc caggtgaaag aacctgtccc tttgccgctg accggctccg 1380
tctgctgacc tccacctccc atcttcactg ctgcaaatcc acctgcaaac taaaggagca 1440
gcatccagtt tttgataact ctcctcgggc ttcagtcaca tcaccagacc ggcgcaagtc 1500
cccggccgta atggccactc tgcgttgata ccactgctt 1539
<210> 26
<211> 1515
<212> DNA
<213> Oryzias javanicus
<400> 26
tcccagcaac acctgtaggg aagagtgtgg atcattcctt tatcacctcc aaagctctta 60
ccaacggcag ccccgccctc acgccctctg caagaatctc agcacttaat attgttggag 120
atcttctgag aaaagtcggg gctctagagt ccaaacttgc agcttgtagg aactttgcca 180
aagaccaagc agccaggaaa agctactcca ctgacaaggg cacgctcatc aacagcaacg 240
ccaccaagtt ctcgcactca atccacacga cttactttga taaaaccact gtcaatggac 300
tggaccccag ctctctgacc tccatggccc catccagagc cgtgtctcct cccggcatgc 360
tgcctttaag tgtgtgaggc tgctgaaacg cctgcccagc caccacggcg cggcgacgaa 420
gaggacagct gtgtgcgtgc gtgagagagt gctcgctcgc tcgcctgctt aagagtttga 480
gtagaattgg ctaaaattca tgttgcagct ataggtgcat ccgtgcttgt gctccttcca 540
catgcgcatg cgcctgcagc tgcactgtgc tcgagtggct gtcattgcaa tgcgcttaac 600
ataaaaaaaa aatccagatt ttcctgacaa aggtcctaaa cagaccacag cttcgtttaa 660
agctcccttt tttctaaacc gcacagttaa actattaaga atgtatgata tttaaaaggg 720
aaacgttcat ctaaactaat ttatctgaga gtctgattcc aaacgctgtt tttctacctg 780
aagttttttg catgtttgtt aagagtgttt actgaagtgc gaatgaccga cgtgaatgtt 840
gacggttctt ggccgcgcgg tgcaagcgtt cctcccgttc ctggctggaa gaagaagaag 900
ttctgaattc ccctcagctg tgtgtctcct ctgcactgaa agaagctgct cctcacaggt 960
ttttggttgc agtggaggtg gttctcgaca tcagctgacg tatttaggaa aacagttatg 1020
tccttttttt ttattgtttt gtgtggatgt tggaagctca ctgtgattcc tcacttacaa 1080
acaacagttt gtgttactgt cccaacatgt ttgccaggtg tagcttcatt tatggttctt 1140
tgctgcgtca cagcagtgca gctaatcttt ctgttcagta ttaattttgc tatatcccct 1200
ttagttttct attttgattt tattttacgc acaaaaactg tatccgcatc catttattta 1260
ttatcaaagc ttgcaagacc attaaaaagt taatattcct gggtgttttt ttgagaaggc 1320
gccctcagag gctgttacgg ctgcagtcac tctgtacgtt tctttaccac tgtacatctg 1380
gcattataga gtcctattta tttctagatg ctgttggcat gtgttccaga gaagttttac 1440
taccatacag tggttacatt gtataataaa gtgtttgttg taatgttaaa ataaataaag 1500
attcagatta ttaaa 1515
<210> 27
<211> 1457
<212> DNA
<213> Oryzias javanicus
<400> 27
aagcagtggt atcaacgcag agtggccgag gcggcctttt tttttttttt ttttctacgt 60
tatttattca tatttctcat tttctgtcag tcattaaatt ttgaaaggtg tactgtcaga 120
aaggcttgtc tttattacac catacatata tatatacaaa aggacacaag gggagggaat 180
ggaataaaga aaggggatgt ttgcgcgatt agaagcattt cctgtggacg atggagggcc 240
cagcttcatc atactcctgt ttgctgatcc acatctgctg gaaggtggac agagaagcca 300
gaatggagcc accgatccag acggagtact tcctctcagg tggagcaatg atcttgatct 360
tcattgtgct tggagccagg gctgtgatct ctttctgcat acggtctgca ataccaggat 420
acatggttgt cccgccagac aggacgttgt tggcgtacaa atccttacgg atgtcaatgt 480
cacatttcat aatgctgttg taggcagttt catggatgcc agcagactcc atcccaataa 540
aagaaggttg gaagagtgtc tctgggcaac ggaaacgctc atttccaatg gtgatgacct 600
gtccgtctgg tagctcatag ctcttttcca aagaggaaga ggaggcagcg gtagccatct 660
cattctcaaa gtcaagagcc acgtagcaca gcttctcctt gatgtcacgc acaatctcac 720
gctcagcagt tgtgacgaag gagtaaccgc gctctgtgag gatcttcatc agatagtccg 780
taaggtcacg accggccagg tccagacgca tgatagcgtg tggcagagcg taaccctcat 840
aaattgggac attgtgagtc actccatcac cagagtcaag cacaataccg gtggtacggc 900
cagacgcata cagagacaga acagcctgga tggcaacata catggctgga acgttgaagg 960
tctcaaacat gatctgagtc attttctccc tgttggcttt ggggtttaga ggagcctcgg 1020
tgagcagggt ggggtgctcc tctggtgcaa cacgaagctc attgtaaaat gtgtggtgcc 1080
agatcttttc catgtcatcc cagttggtga tgatgccgtg ctctatgggg tacttcagcg 1140
tcagaatacc tctcttgctc tgtgcctcat cacctacgta gctgtccttc tgacccatac 1200
caaccatcac accctggtgg cggggtcttc caacgatgga aggaaaaaca gcacggggag 1260
catcgtctcc tgcaaatcca gctttcacca agccggagcc gttgtcacac acaagcgcag 1320
ttgtctcgtc gtcgtcacac atggttgagc tttaccggcg ggggtctaca atttgagctg 1380
gttgctcgcc aacgtacgag gatgcttcca ctgggacccc cggccgtaat ggccactctg 1440
cgttgatacc actgctt 1457
<210> 28
<211> 1412
<212> DNA
<213> Oryzias javanicus
<400> 28
aagcagtggt atcaacgcag agtggccatt acggccgggg accccacaac tgctaacagc 60
cgtctactgc aacaaaaaca cggcggttct tggaaaagac atttaaaaaa atgaggagca 120
gcttctgtgt cctgcagtgg ggaggtgcac tcctcctcct gttctccctg ttgcacgtcc 180
attcggaggt cccaccactt gaggagcaga ttgtgggcag tgcagctcac atacccagcc 240
accacacgcg tctggacaag aacatggtcc aggatcagga tcacatcatg gagcatttgg 300
aaggagtcat agacaaacca gagacagaca tgacaccgca ggagctgcag ctgcattatt 360
tcaaaatgca cgattatgat ggcaacaacc tgctggacgg tctggagctg gccacagcga 420
tcacacacgt ccacagagag gaaaaaggag agcacagcca gcccatgaag gaggaggatc 480
ttattgctct catcgacgac gttttaaaag atgacgacaa aaacaacgac ggctacatcg 540
attatgccga gtttgccaaa tctctggagt agagtggatt cggttgggag gacttgttag 600
aaaagggaga acagcgtgaa gacaaaggga aagagcccac cacatcttcg tttctgtcgc 660
tcacgcttga taatcagggg gggtgtctgt aatttaagct ggttcttttg taccggctgt 720
aggagcacaa ctcatcactc tagatatcgg atcaacaaat gtgctggaat ctgggtcatt 780
ttgcatttct gtgtggtcct gtccgtttca gctgttttct gccaaagtcc aacagtccat 840
attgatcggt tgctcaaact aaaactaatg ctgccttcat ttaccaggct gctctgtagc 900
gttcactaaa acatcgtcaa attaacaaaa gtatttacgc gttgacacgt tgtttgatgg 960
accaaacgag gccgtctgtg agccggttca gttacgacag aaacgtggcc ttcacttgat 1020
ccaggatgaa tgaagcttga actgatcgga tgtttcctgc ttgagtattt ccacaatgag 1080
cccaccttca gttggtgcag ccagacctct cctcgtttcg cctctgaggt gaagcggcgc 1140
cgcactgacg tacgtcactg ccgtgacagt aaagcacttg aatgtaactc gtctgtttca 1200
cacgctcgct gcctttaaaa cacgtcaggt caaagtttgt tgaaaccatc tgtgatttct 1260
tctgaagcgt tttttcatgt agatgaatgc tatatttttg ccatccattg gtgattcttc 1320
agatgtaatt caatgaaaaa tatttaaaga taaaaaaaaa anaaaaaaaa aaaaaaggcc 1380
gcctcggcca ctctgcgttg ataccactgc tt 1412
<210> 29
<211> 1400
<212> DNA
<213> Oryzias javanicus
<400> 29
aagcagtggt atcaacgcag agtggccatt acggccgggg gaattgaaag agacaggagc 60
tcggtgaaaa catttttttt ttaaactttg cttcaaagca tctcctactg tcatggcaat 120
gcttcttctg aaacgcttgg cgaaagagca tgaggattac atcaaacaac aagtccatgt 180
gaaaaatcct tatcttgact ccatggagga ggacattctg taccacttca gcctgagtac 240
aaagactcac aacctcccag agatgtttgg agatatcaaa tttgtgtgtg ttggtggaag 300
cgcgaatcga atgaaggctt tcgctcactt catccaccaa gagctaaacg ttcctggaaa 360
cccagacgac atccaagaca tctgtgaggg aacggaccgc tactccatgt acaaagtggg 420
gcctgtgctt tctttaagcc acggcatggg cgtcccatcc atctcaatca tgctgcatga 480
gctcatcaag ctgctgcatc atgctcactg ccaggatgtg gttttgtttc gtctgggaac 540
atctggagga gttgggttaa atccagggac ggtggtgata acagaaaaag cagtggacta 600
ctctttccgc ccccagtttg agcaagtggt tctgggtaag gtcatcacca ggagtacaga 660
gttggacgaa gaggtggcga gtgagcttct gcagtacgcc tctgaactcg agagctttcc 720
aactgttatt ggaaacacca tgtgcaccca tgatttctat gaaggtcagg gccggctgga 780
cggagctctc tgctctttct ctcacgagga gaagctggag tatctgaaga aagcgtatga 840
agctggagtg aggaacatcg agatggaatc caccgtcttt gctgccatgt gccgcgtctg 900
tggcctcaaa gctgctgtgg tctgcgttgc gttactgaac cgctttgaag gagatcagat 960
cacctcttct catgaggttc tggtggagta tcagcagaga cctcaaatcc tggtagctta 1020
ctttatcaag aagcggcttg gacttattcc ctgaagcccc ggatatttaa ataactgtaa 1080
atatgtaaca tttagacatg tatatatcga ttggtgatat ttgaagtaag cacttattta 1140
agtcttaaat gatttattca ataagccaaa tgtattgggt ttgttggatc tttttttttt 1200
tttaaatcct ttgatattta tgtgaggaaa taaaaatatt atgttgagtt gtttctatat 1260
tactgcaaag gttgtaaatt gcacacatga caaatatgaa ataaacttca ttaaatcctg 1320
aataaagttt atttactata gaaaaaaagg aaaaaaaaaa aaaaggccgc ctcggccact 1380
ctgcgttgat accactgctt 1400
<210> 30
<211> 1354
<212> DNA
<213> Oryzias javanicus
<400> 30
cagtggtatc aacgcagagt ggccattacg gccgggggtg ttgtccctcc ctgaatcgcg 60
ctgttatcgc attcgtggct acttgcgtac acaatagccg ggttcggcaa gatactttgg 120
gctcttaagg acatcgcgag gcgaaacctt gtaacgttat gcctcaaaga cgcagctaaa 180
tcgctttagg atggctttct cggtgcggat gatcggcaaa gttgggacga gacttcgggg 240
aagtcttcgg actcttcact gcggtttcac ccgtcctcat ctggcgacat ttctacacca 300
agttcatcac aggcaggctc cctttggaag tcgcagagct cttctcttca gtacactagc 360
tccctccagt gacttgtctt tggagtataa acgtggccgt ctggtgctgg aggtgccact 420
gccatccaga aatgagaagt gtctgttctt cctcaggccc atgctgatga atgtgggaga 480
tctgattact gatctgcagc gggaggactc tggagtgacg gcggctgttc tctctaaaga 540
tggggagcgc gttgctaact ctacactaat agaagccctc ctgggaaagg actttctcct 600
cgtcattaat gatatttgct acaacgtctg ccctcctgaa aaagcttttg caaccagcga 660
gcatgccagg gacatggagg acatgaaaca catcgtgcac ctgctgcaca cggctctcca 720
cctgcccgaa caccacctcc tgaaagagcg gcagttactg gagaggctgg ataacctcaa 780
acaggagtta acacctctgg agaagatgaa ggcacagctg tcccgtacag ctgagttcca 840
cagctccagg gttctgtgga ccggcatggc actgctttcc gtgcagggcg gggctctggc 900
ttggctcacc tggtgggtgt actcgtggga cgtcatggaa ccggtcactt acttcatcac 960
gtatgcgacc agcatgggag tttttgccta ctatgttctg accaaacagg attatgtata 1020
tccagacgcc aaagacagac agttcctgca ttacttttat aagggggcca gaaagaagga 1080
gttcaatgtg gaaagataca atgaactgaa ggaagaacta gctgaggtgg aggaggatct 1140
gaggcgcctg agaaatccaa cccagctcca gctgccacta gaacagatcc agccaaagcc 1200
ataaatgaaa ccaagctgag agcaattctg aaactcatcc ctttttattt gatattttca 1260
aactggaatc cagaggacca tgagttgttt agtgttatga tgatgatgcc aacttgtacg 1320
attggaaaaa atgtaataaa aaaggtgttt tacc 1354
<210> 31
<211> 1333
<212> DNA
<213> Oryzias javanicus
<400> 31
aagcagtggt atcaacgcag agtggccatt acggccgggg gcttggcagc tggtgagagc 60
aaggacggtg gtgtcgacgc tagaagtttc tttgcagaca ataaccatcc aattcggaat 120
ctaaaacctc ggaataagga aactggacac gtttgaattc gcttaaatta ctcttcagca 180
gctgttgaat actgcaacca tgccagaagt ctttgcaccc gcagacatgg gcagctcacc 240
tccatccccc gtggacagca gtccgaggag actgtcctgg ggcaaactgg tccacaggtt 300
gacagatttc agcacaaaca gcagcagtat cacgtcaggg tccaacaatg gcagcaggag 360
tgacctctcg gaatctgggt ccgatacctc cagtgacctg tcaccgtaca atgatgacct 420
cttcttcgat ccgatggagg aaaccatcct gaaagaagtg gtggacctca tcgcatccag 480
ccttagagaa gccaaagact cggactgtgc tctgaggtgc gccaagctcc tcattcccga 540
gaaactgctg gagcacatcg gacgggagct ccttcatctg gctgccagcg agccctgcgg 600
attgaggggg gctctcattg acctttgtgt ggagcaagga gatgtgtgtg agagcatggc 660
tcagctatcg gtggacccct gcctcgtccc cacttttcag ctgactttgg tgttgaggct 720
tgagtccggt ggcttgtggc caaaaattca aggacttttt agctccagat ctacttccac 780
tccagcagtc cgacaagcac ttaaactcag cactggtttc cgcgtgatca aaaagaaact 840
gtactgttct gaagacctgc tcattgagga atgttaagaa atgaaagact cgtttttttt 900
ttaatataat gcgagcttac aatgaaagct caaagaaggg ccttttaaga ttatgcctac 960
agaagtataa agaaggactt tgttctgatg tagcctttgg ttcctgctgt aagtttctgg 1020
tacttggatg tcttcatgca gcacttgctc aaaacttaag tttaaaaaat gtaaccatat 1080
aatatctggt acatctgcat ttgaaactga atttgaattt taaatgcacc gacgtttcta 1140
ctgaaacatt gaaaaaggca gctagctgta ttgtgttacg cattgaagac aactgaattg 1200
tacggcaact catgacatgt actccaattc ctcagtttgg gaacatttaa gctaaagctt 1260
gcacatgtgt aagcctccac tttgatatca cgtctaagtt tttttttttt ttttgttttt 1320
ttttttcttt tac 1333
<210> 32
<211> 1214
<212> DNA
<213> Oryzias javanicus
<400> 32
gcagagtggc cattacggcc gggggagctt ccgccttgtc accttagctg aggagaaaat 60
cgaaggagcc gcagtcagga gtttgaagaa ggcttgtaaa taaggaaaat acagtctggc 120
tctcacagag ggtttgggaa gctgagaatg gatgtgagga aggtgaaaaa gatagttctg 180
agtgtggagc aagcagaagg agttggtgct cgagtccgca gaagcattgg cagaaaagag 240
ttgagaaatc tggatccttt cctgatgctg gatgaattca aagtgagcaa accagcaggt 300
tttcctgacc acccccacag aggatttgag acggtcacat atgttctgca ggggaccacg 360
gctcatgaag acttctgtgg tcattcggga gttctgaagc ctggggattt gcagtggatg 420
actgcaggac ggggagtgat gcacgccgag atgcccgtgt cagatgagcc tgtggtgggc 480
ttgcagctgt gggtgaactt gtcaaagcga gataagatgg tggagcctgc gtaccaggag 540
ctgaaagggt cggaaatccc taaaccgagt aaaggaggag ttacggtggc tgtgatatct 600
ggagaagctt taggagccaa gtccaaggtc tacacaagga cgccaaccat gtacctggac 660
tttacattga agccgggggc cgtgcatgtg cagccagtct ctttgggatg gactaccttc 720
atctacacgc tcacaggaac ccttcatgtt ggccctgatc aggagcaaaa gcaggtggag 780
ccgcatcaca ctgtggtgtt cacagatgga gactgcgtta agcttgaaaa caagggttct 840
ggagattccc attttgtgct cattgccgga gaacccatca aagagcctgt agttcaacac 900
ggtccgtttg taatgacttc agaggaggag atcacacagg ctttgactga ctaccgcact 960
gggacaaatg gctttgaaag ggctttgaat tggaggtcaa aaataggaag ttctttctga 1020
acattttaag gtcaaaaatg tttttattta tcttcatttt aatgatcact aaaatgttta 1080
atattcacaa atatattggc aattttgaat tttggtctgt taaggatgaa gtatgtagtg 1140
agttccaatc ttttatttat tagttttagt gttttattaa tcttccacca ttcctgtttg 1200
gctttaaaca taca 1214
<210> 33
<211> 1198
<212> DNA
<213> Oryzias javanicus
<400> 33
aagcagtggt atcaacgcag agtggccatt acggccgggg aacgacggaa gacgtctccc 60
ccgtgatacg gattactgtg aatttctcaa aatttaaacc ccttttcctt cctcttttgt 120
gtcaagacag catcctttcg ataaaatatt tgttttatta actatttcaa aagccaaagc 180
atgtccaccc cggctagaag aagacttatg agggatttta aacggctaca agaagacccc 240
ccagctggtg tcagcggagc cccatctgaa aataacatca tggtttggaa tgctgtcata 300
tttggccctg aaggaacacc atttgaagat ggtacattta aactcattgt agaattcaca 360
gaagaatacc ccaacaaacc ccccacagta cgatttgtgt caaagatgtt tcatccaaat 420
gtctatgcag acgggagtat atgtctagac atcttacaga accgctggag ccccacgtac 480
gacgtgtcat ctattcttac atccatccag tccctgcttg acgagccgaa ccccaacagt 540
ccagccaaca gtcaggcggc gcagctgtac caggaaaaca agcgggagta tgagaagcgc 600
gtgtccgcta tcgtagaaca aagctggagg gacagttgac ctgacaatcc tgctagctgc 660
tatattaagc tgtgcgagct ggaagtttta gtggactctc ttgttacccc ctcacctttt 720
ttttaatttt ttattttaag ctttttatgc actttacctt aatcttccac tcagttttct 780
tttgtaacca cttttttttt taaatatttc accctcttct aatccttcag ggggaaatgt 840
gttgaaactt gccttggtat tttgtcagaa ctgatttgag gattacaaaa actatttgct 900
tataacgatt cataaggggg cgtcaatcaa gtggtgggtc atttctgttg atcggttttt 960
ggttctgaag gaagtgagga tgatgaggag tcttggaatc aagccccaca actgaatcgg 1020
gcatctttct gctgtgtctt tgctgtgaac tgttacatac atgcatgatt aaatgttctc 1080
ctgacccgtt cacttctgca tacaacagaa acctgcaagt gaaattatgt acagattttg 1140
cactggttgt cccccatgta agtcttttcc agaaattgta aataaacaga caaaatta 1198
<210> 34
<211> 1198
<212> DNA
<213> Oryzias javanicus
<400> 34
tgtttcacat attttatttt tcccatcttc agacaaaaag ctgttgaatg tttcagtgtt 60
tctattggcc tgttgctgga cagttcctcg tggagaagcc ggacaccacg ttggtcacct 120
taaccacctt tggaggagcg ggcatggtga tgacaccctt cagatagcag tggcgccgag 180
ctgcagattc gggggagttg tccttgacgt aggtgtagaa ctgacaggtg gggtctccag 240
agcacagcct ctggcactgc tctgcattat ccttcagctc gaaacggatc tctggaccag 300
gaaaatctat tccctcgtaa gccgtcctga gccagttggt aaactggcag aagcgtgcag 360
gcatccctga tgtggctccc tctttaactt tagtctccag gtgatgtttg ttgttcttca 420
aataacattt aaagtcaaga ctgccaaagg tgaagcaagt gcattgtggg tgagcggagc 480
acagggcctg acagtgttct gcagaggcgg ccttcagcgt ctggaagtca cttcctggga 540
tgtccgtgtt tgacaggaac ttgttctgac agactgtctg agggggattc ggtgtttggg 600
aaaatccaga gaccacgccg gcctttttct ccacggagga agcctttggt atattccagc 660
tgtatttgag gaaacatttg tacctgacgt tctgctgttt ggggtcctgt ttgacaaacg 720
tgaagaactg acatgaagga tcctgagtgc aagctttctg acagtcctca tagtcggctg 780
tgaagaagga ggtgtactct gcccccacaa agtccacgtt ctcgtagacc cgaggcagac 840
acggttgtgg gcttggattg caggatttga gggagaagcc ggaggtgacg ccctgaaggg 900
gagtttgaga agtgggttgt ccagaagaag ctgatttgag aaagcacttg aaggtcctgt 960
cgtctctggt ccagtcggct cggtggaagg tgaagaagcg gcaggagggc tgctgggtgc 1020
acagcagctg acagtgctcc acgtcaggag aatagaaggt ttttatgtca gatcctggga 1080
agtccacatt ctgctgcagc tgcctgttgc acccttctgt gaatgcaacg ctgcagagag 1140
acagcaggct cacccaaacc agccccccga tcatcctgtg aagctgatcc tgcaggaa 1198
<210> 35
<211> 1160
<212> DNA
<213> Oryzias javanicus
<400> 35
aagcagtggt atcaacgcag agtggccatt acggccgggg ttttcggttt tttcctttta 60
agtctaggaa gaaagtcagg ctatttgact tgaatcaagt gagaagaaga aagtgagtcg 120
ggatgactgc cctcctcctg cccagtctcc tgctggctgc tccgatcctc ctggtggcgt 180
ccggccctct cctggctcca ccaacaacac ggtgcctctg gttctgtggc acgggatggg 240
cgacagctgc tgcaacccgc tcagcatggg tgccataaag aagatgatcg agcaggagat 300
ccctggcatc tacgtgctgt cgctgatgat tgggaaaaat gtggtggagg acacagagaa 360
tgggtttttc ctggacgtga accaccaggt gtcgatggtg tgcagtcagc tagctcagga 420
cccaaagctg caaggggggt acaacgccat gggcttctca cagggagcgc agttcctgag 480
agccgtggct cagcgatgtc cctccccacc aatgaagaag ctgatttctg ttgggggcca 540
gcaccaaggt gtgtacgggc tgcccaggtg tcctggcgag agctcccacg tgtgcgacat 600
gatccgcgag gctctgaaca ggggggcgta cagcgacctg gtgcagaagc acctggtgca 660
ggcccagtac tggcacgacc cgctcaatga cgacctctac aagaagcaca gcgtgtttct 720
ggcagacatc aaccaggaga ggaccgtaaa tgagacgtac aagaagaatc ttcagcagtt 780
agagaagttt gtgatggtca agtttctgca ggactcggtg gtggatcccg tcgacaccga 840
gtggttcggc ttcctgaaaa caggccaggc caaagagacg gagactctgc aagagagcga 900
cctctacaaa caggaccgct tgggcctcgc tgccatggac aaagccggaa agctggtctt 960
cctggccacc gagggagatc atctgcagtt ctccaaggaa tggttcgatg ccaacctgct 1020
gccgtacctg aggtagaggt gcacggaccc gccccctcca cccgccatga gctcctcttc 1080
ccgctgcttt aacagatcaa agttttctaa caataaaacg tctctgctgt gaatctgatc 1140
aataaagaca caaactctaa 1160
<210> 36
<211> 1160
<212> DNA
<213> Oryzias javanicus
<400> 36
aagcagtggt atcaacgcag agtggccatt acggccgggg gtaggtcagg agggacgcca 60
tgtgctgttg aggcaggggt gcaggagaag gaccgggctg gaccagcaga acagattcgg 120
ttgaaaattc agcgggacag cggggagacg tattccgtga acgttagcat gtactgcacc 180
agccgctgcc tccggagcgc gctgcgcgcc gccgccagca cgcaccggta ccgacaacag 240
ctccaacatc aggttccgtc cttgtgtgcc ctcagacttc tgaagaccag ctcatcctct 300
cattcagaaa ctattgccac gcctccatta gacggagcac ccaaacagta ctcccccaaa 360
attcagcagc tcgtcaacga cattgccagc ctcaccttac tagaggtgtc ggatctgaat 420
gagcttctga agaaaactct gaacattcag gatgttggaa tgatgccgat gggggcaatg 480
gcagcaggtg ccatgccagc aacgccggct gcagaggagg aggaggcacc ggccaaaaaa 540
gagaagactc acttcacagt caaattgaca gaactgaaag cagcagataa agtaaagtta 600
ataaaagaag tgaaaaattg catccaaggc ttgaacctgg tccaagctaa gaagcttgta 660
gaatctcttc cacaagaaat ccgggccaac gtatccaaag aagaagctga gaagctgaaa 720
gcagccctgg aggctgcagg cggcactgtg gtcttggagt aaatcagtgc atttctacat 780
ctgcctctta cattgccatt tctctggcca cgcacttcgt ttttcattat tttataactt 840
tttttttttc ctgggcgaag acgtggacac tgcaaaagaa gcaaatgcct tcatttactc 900
gaagcgtcca tagcatcagg tggggtgctg cagattgaac ttttccaggg acacggcctg 960
cacaaaccac ctccttgcag tttgtttgtg tgctcccgtt tgtctgaaac tcgtgtagca 1020
aacgctacca aaactctggc ccccggtgcc aggcggtatt tctgtgtgtt tgtcaagccc 1080
ttcataaagg gacggtttcg ctgtgaaagt aggaatactg gaacagcagg aactgtattt 1140
gactctgaaa tgagtagtta 1160
<210> 37
<211> 1157
<212> DNA
<213> Oryzias javanicus
<400> 37
gccggggggc tttaaaaaaa taagaacaca ttgtaaacat ttctccgttg ttctgaatcg 60
aaatggctcc gatagaccga aaaacgacga ataaaaactt tacgaaaaac aatagaaact 120
ggaaaaaggc aaagaatcgt cagctggcgt gaaaaagaac cggaaatgga tttcacagca 180
caaagtattt gaaggcagcg ttaaagaagg tcaaggattt gctcttaaaa gaaaacagaa 240
agttaaatat gaatacaaca aactattgaa aaaggagaag aagagaaatc ctgattccaa 300
aatgctgtac aaggaggagt atccagaaca cctcaagcat ctgtacatgg cagaggagga 360
gaagctgaaa aaagaagccc gggaaaacag agtaaacagg actaagcata gaatgcaggg 420
gcaggggacg agggacgaaa acgaagcgga tgaagacgtt tctgggggac ctgagccgac 480
aaatcccata cctgtggccc cggagcaaac ggcagacgct gagaaagaaa gccttccaat 540
gagtaaccgc atgaagaaga aactgcagaa gaagacgtcc taccaaaaga caaaagagga 600
atttgagaaa ataaaagaaa agcgaaagaa gaaaaagagg aatatctcaa aaacaagcag 660
cagagagaag aagctattaa gcggtacaaa cagaaaaaga tggaaacgtt tcaaattctg 720
agcaaaaaga ccaagaaagg acagccgaac ctcaacctgc agatggaata tttacttcag 780
aagatccaag gaaagcaaaa atgacgacag aaaattgagc aaactgttaa atatttaaaa 840
taaaagttct cgcaaatgca atatattatg tttttgagta attctgttat agaattaatc 900
attttacaac caaaaaaagt aatttttaca ccacaagatg gcattgttag atacaatgtg 960
cagcttctgt ctatactgat aatgctttta tttaaagtaa acaaagaata aatgtttggt 1020
caaatatcta aactgttatc taaaaatgaa taacaaatca cagttttttt tagaagaacc 1080
tatttgggat tgaattgcta ttttaaaacg gagatcttca tattattgtg aattgttcat 1140
taaaacgtgt ttgtggc 1157
<210> 38
<211> 1137
<212> DNA
<213> Oryzias javanicus
<400> 38
gcagtggtat caacgcagag tggccattac ggccggggag aaaggattgc cacactcgca 60
aaacacgtta gtcttacctg tggcagcagc tgtcatggaa cacattaaag atgggtggtt 120
tacggagtcg tgcgcgctgt ggccgggaca agcgatgagc ctccaggtgg aagaggtcct 180
gtacaacaaa aagtccaagt tccaagatgt tatggttttt aagagtaaaa cctacgggaa 240
cgttttggtc ctggacggag tgatccaatg caccgagaga gatgagtttg catatcagga 300
aatgattgcc aacctccccc tctgcagcca cccctgcccc aaaaaggttc tggtgattgg 360
tggaggagac ggcggtgtgc tccgagaagt ggtgaagcat cctctggtgg agtccgtggt 420
tctctgtgag atagatgagg atgtcatcaa tgtatcaaag aagttcctac caggcatggc 480
taaaggcttc ttcagtccca agctgacgct ccacgttggc gatggctttg aattcatgaa 540
gcaaaaccag gacgcctttg acatcattat taccgactcc tcagaccccg tgggacctgc 600
tgagagtttg ttcaaggaat cttactatca gctgatgaag gcagctttga ggagcggagg 660
aatcctttgc tcccagggag agtgtcagtg gcttcatctg gagctgataa aggagatgcg 720
taccttctgc aaaactcttt tccctgttgt ggattacgca tacggcacca tcccaaccta 780
tccaagtggc caaattggat tcatgctctg cagtaaaaac cctgaaacaa acttcagaga 840
accggtgaaa gcactgtcaa aagaagaaat ggagactatg aacttacggt attacaacac 900
tgaaattcac aaagcatcat ttgttctacc tgagtttgct agaaaggtac tcagtgacgt 960
atgaccaaca acaactgacc tccatgtttg ttctctacct ggcgctccga actcagacac 1020
aagcaatcta acttaaggac tccacctcct ccgtctgtca cctcagagat ccgattgtga 1080
aaacctgctg agtttgcgat ctagtagcag cgtttcctat gttgttcctc atgtttc 1137
<210> 39
<211> 2040
<212> DNA
<213> Oryzias javanicus
<400> 39
aacatcgcta ttgagacaaa aggacggacg ccataactca aaccttcggc ttttgatcca 60
agtctcggag gaaaaaagaa aggacaaatt ttctatcgtc tcgcgagaaa cccgtcgatt 120
tttaacagca atggctcaag agacaaacca gagcccggtc cccatgcttt gtgccaccgg 180
ctgcggtttc tatggcaacc ccagaacaaa cggcatgtgc tctgtatgct ataaggaaca 240
cctgacgcgg cagcagagca gtgaccgcat gagccccctc agcccaatgg gctccgctgc 300
tagtcctacc tcagaggtct ctgctatcca gagactagaa gccagtctag ccaaggttga 360
tgcctcccct gcctcttcac cagacatgtc tagaactatt caaggatccc ttcctgtgac 420
tcagcaaatg acggagatga gcatttcgag agaggacaaa cccgaatcct tagagcctgt 480
tataaaccag cctgctgctt ctagccctac tcctgtagct tcttccagtg ttgaagaaaa 540
taagggcgac actcccaaac ccaaaaagaa cagatgcttc atgtgccgca aaagggttgg 600
actcacaggt tttgactgtc gctgtggtaa cctgttctgt ggcattcatc gatactctga 660
caagcacaac tgtccctatg attacaaggc cgaggccgcc gctaagatcc gcaaagagaa 720
ccccgtggtt gtagctgaca aaatccagag aatataagtt tgacagagca atggagctgg 780
caacaatgaa acaccacgac aaagacttga gtgtgattct ttgacaaaag cgccgacaca 840
agtggctaat tgaggatgga caggacttga tttaccaaat cacaacctca taataaccgg 900
aaaaagaaac aatttgagat cctaagcgag ggagatgcat gaatgtattt tttttttatt 960
aatccattaa attgttttat cctgtttctg tggtgtgact ttgccgctgt tatgtccttt 1020
ctttttctaa gaatatgtat tgtcctattt agagcagacc aggctaactg caggatgcgc 1080
tgtcctcctc tcacccttgc caggcgccat tttgtgccaa aacagccatt ttagccagac 1140
tttccagtgt tttgaacagg ggcaatgttt tcagactgga atcatttgcc ctccctgcac 1200
gagacaattc gacttcatct ttgttacctt tacctacctc acagctactg cgttaagcta 1260
cgttgtcttc ccttttacgt tatacaacag ttggatcatc tgtcgaatga tcgcaccttt 1320
agcttgaggt atttcgccag acgggtacaa aggaaatcac ttagcatttc acagaatgtg 1380
aacatttgcg gtggcaaccg tgttgcaggg tttaacagcc tgggttgagt tcacattgta 1440
acctgacgcc tggcaaaggg ctgtgtgcac tcctgtgcag cttgagagga agagcagcat 1500
cgattcctgg tgggcctttt gggcgaactt aggctagggt tataaatgaa aaaggggtat 1560
caatgaatga aataagtgca tgagttactc ttcattttca ctagtgtttc aaaagaatca 1620
ttggggaaaa aagttttttt gttttcctga gaaattgttt ggattatttt ctttggtctc 1680
catagtgctg gtgtttgcac atggatttta gtgcatctgt gggagtattt ttttcttgtt 1740
tttttttctt tcattgcgct aacacaaaat tcatgtaagc acagctgtgt gtgtgttggt 1800
gtgagtgttt tgctgcaccc ccgatcctat ataagttgtg gagctgcccg gtttatcagc 1860
acatgtttac agaagttaaa ggagcatgcc cgagctagat ttatgaaaaa taatagaaat 1920
caaaattaga tgtaaccttt catcagttta attgctatca gtctgtttat ctgctataga 1980
acactgtaga gaagcgtgtg tgagagtaat tgtgggcgag catgtgcacg tacccgtgca 2040
2040
<210> 40
<211> 1079
<212> DNA
<213> Oryzias javanicus
<400> 40
gtggtatcaa cgcagagtgg ccattacggc cgggatgcgg gatgaagggt tcacggtggt 60
tgccaaagtg gacccagcca caggtttttg tgacgggggg gaaccgcttc aactgtggga 120
cgtggatgga caaaaatggg ggagagcgac cgagcacgga acaaaggcat gcctgcaact 180
cccagagacg gcgccgcggt ggagatcgtt ggtctcagta aatcagctgt tcgttggctc 240
gtggagctcc acgccaaagg actgttcccc tacgatggag ccaacgttca cagagacggt 300
aaagaggtgt tcatctcgta ctctcagtgg aaccagcaac tccagcactc ctttgaaaca 360
gagttctggg tgcccgagga tcccaacgac cccaatgaga aacaccccga cctggtgcat 420
aaaaaaaggc atttataagg acagctatgg agcttccagt ccctggtgtg actaccaact 480
gagacccaat ttcaccatcg ccatggtggt ggctccagag atgttcacac cgcagagagc 540
ctggagggct ctggaggtgg cagagaagaa actactggga ccactgggaa tgaagacact 600
tgaccctgat gacatggttt actgtggtgt ctatgacaac gctctggaca acgacaacta 660
caacctggca aaaggcttca actaccacca gggaccggag tggctttggc cggtcggcta 720
cttcctgcgg gctaagctct actttgccaa gaagctcgga gaggacacct acaccaagac 780
catgacattg gtgaaaaatg tcttatctca gcattacact cacctggaga ggtctccatg 840
gaagggcctc ccagagctga ccaatgagaa cggccagcac tgtcctttca gctgtgagac 900
gcaggcctgg tccatcgcca cggtgctgga ggtcctcttc gacctgtagg gcgagatgcg 960
ggacggccga ggccgctcca tccacgccgc cgccacgaca cgattttaca caatgagtgt 1020
tttccctgct tctgtcctgt gccctgaggg agggggatga aaggaataaa accatccga 1079
<210> 41
<211> 1042
<212> DNA
<213> Oryzias javanicus
<400> 41
aagcagtggt atcaacgcag agtggccatt acggccgggg agggagctgg aagttactca 60
tctaaccgtt tgcacccgtt ctttagcatc gaactatcgc tacgttcacc agccgagtga 120
gactaaacat ggcggcctac aaattggtgc ttattcgcca cggagagagc aactggaacc 180
aggagaaccg tttctgcggc tggtttgatg cggacctgag tgagactggg gagcgggagg 240
caaagagagg aggacaggcc cttaaggatg ctggctacga gtttgacgtt tgttacacct 300
ccgtgctgaa gcgagccatc agaacactgt ggctggtctt ggacggcatc gaccagatgt 360
gggtgcctgt gcatcggacc tggcgtctaa acgaacgcca ctatggcggc ctgacaggac 420
tcaacaaggc agagacagct gccaagcatg gagaggctca agtgaaaatc tggaggcgct 480
cgtttgatat ccctcctcct cctatgggcc ccgaccatga ctactattca gtgatcagca 540
aggatcgacg gtatgcagac ctgactgagg accagctgcc gtcctgcgag agcttgaaag 600
acaccattgc gagagctctg cccttctgga atgaggagat tgcccctgag attaaacaag 660
gaaagcgagt tctcatcgct gcccatggaa acagtctgag gggaattgtc aaacacctgg 720
aaggtatgtc agatgaagcc atcatggaac tgaatctgcc aactggcatc cccatcctct 780
atgagctgga caagaacctc aaaccggtga agccgatgca gttcctgggg gacgaggaga 840
ctgtacgcaa agccatggag gctgttgctg ctcagggaaa ggccaagaag tgaagaaacg 900
ggaagcccaa aaagtcatct ttgtctgttc ttaggtttac ccaaactgct ttcttcagat 960
gattttttct ccctaaattc tggtttattt tggtttagaa aatgctggat gttgtgtaga 1020
ttgttgtcaa aaataacctt gc 1042
<210> 42
<211> 1011
<212> DNA
<213> Oryzias javanicus
<400> 42
tggagattac gtcttccagg ttgtgattaa cccaaactat gaggtggcag aatctgatta 60
caccaacaac gtacatgaaa tgccaaacgc gatacgatgg acaccggata tggacgtaca 120
actgtcatat aggtggaacg ctgagtacag acatggacga caacacgttt cctggtcttc 180
agaccaacca gttttctcac aggtagaccc ggaacggact gaacaggagg gcggcatcag 240
ggaagcgtgc gctcatggtg aacgaccact agggggcagt gcgatgctgg actgttgctg 300
agctcagggc tcacacggtg aaacagaaga ctaatccatt tatattcttc tggatgcagt 360
ggtgctcaac agatgcaata cggactgaag ccaaataaag aagaaacttt gtgaacattt 420
ttttagacca aagagtcaat tctagcgtaa ctgtgagtgt ttgttttgct ttgaaatgac 480
aaaaattgtg ctgtcagaga gatgggaagt tatttatcct caaaccatca gcaggattct 540
cactttaact gcagagaaat ggaattctct ctaaagttcc cctgtaagga ggagctaacc 600
ctgaaatgtt tgtgataata tctgcagaat aaacaaccca aactaggatg aatatctctg 660
ctgccagagg cgacgagtaa acggagcagc aaatgaaaca gatttgttca tcttttgaag 720
actttcagga agaaactcaa cagctccgtc tttttttttt tcttttgctt tacgtcatga 780
aacggcatca tcacatctgc ttctatctga gtctgagctt tgttaaatta ttccagtcag 840
ggatgtggag tactgtagcg gccgcacgtt cgtgaaaatg cctcctcgtg gatttgtaca 900
ttagtttttt caaatgcagc accggcagaa tccttcattt tataaggacg gctgattgtt 960
gctaaatgtt ttcatttgtc tgttaaaaag aaataccttt tgaataaaaa a 1011
<210> 43
<211> 2040
<212> DNA
<213> Oryzias javanicus
<400> 43
tgttgttcac attatgcctg gatattccga cagagatcgt ggcagagaca gggatagaga 60
cagaggttat ggcggaggtc ctcgttttgg tggaagtcgc ggcggcggtg ggggaggtgg 120
tggaggaaag tttggtaacc ccggagaacg gcttcggaaa aaacactgga acctggacga 180
acttcccaag tttgagaaga acttctacca gcagcacgcc gatgtggccc ggagaccact 240
gcaagaagtg gaacagtaca gaagaaccaa ggtgattacg gtgaagggga gagattgtcc 300
caaatcccat cacgaagttc catgaagcaa gcttcccatc ctatgtgatg gaggtcatca 360
acaaacaaaa ctggactgaa cctactccga tccaggctca aggctggcct ttggcactga 420
gtggcaaaga tatggtcggg attgcacaga ctggatcggg caagactctt tcttatctgc 480
tgcctgcaat tgttcacatc aaccatcagc ctttcttgga acgtggagat ggccctattt 540
gcttggttct ggccccgacc agagagctgg cccagcaggt gcaacaggtg gctgcggagt 600
acggcagagc ttctcgcctt aaatccacct gcatctatgg aggagcacca aagggacccc 660
agatccgtga cctagagaga ggtgtggaga tctgcatcgc cactccaggc agacttatcg 720
acttccttga atcagggaag acgaatctgc gcaggtgcac ctacctggtg ctagatgaag 780
ccgacagaat gctggacatg ggcttcgagc cgcagattcg caaaatagtc gaccaaatcc 840
ggcctgaccg gcagactctg atgtggagcg ccacttggcc caaagaagtt cgccagctgg 900
ctgaggactt cctgaaggag tatgtgcaga tcaacgtggg ggcactgcag ctgagcgcca 960
accacaacat cctgcagatc gtggacgtct gcacagacgg agagaaggaa aacaagcttg 1020
tccgtctgct ggaagaaatc atgagcgaga aggagaacaa gacgatcata tttgtagaga 1080
caaagaggcg ctgcgacgat ctcaccagga ggatgaggag agatgggtgg cctgctatgg 1140
ggatccatgg agataaaagc caacaggaaa gggactgggt tctcaatgag ttcaaatttg 1200
ggaaggcccc gattctcatc gccacagatg ttgcctccag aggtttagat gttgaagatg 1260
tgaaatttgt catcaactat gactacccca acaactctga ggactatatc caccgcattg 1320
gcagaactgc tcgtagctca aagacgggca cagcctatac cttcttcacc gccaacaaca 1380
tgaggcaagc cagcgacctc atcgctgtgc tgcgcgaggc taaccaggcc atcaacccca 1440
agctcctcca aatggctgaa gatagaggag gtcgttcaag aggtggctat agggacgatc 1500
ggcgggatag gtattcatct ggtaggaggg attttggtag ggaccgggaa aacagaggct 1560
tcgacaacgg acagaataaa gctttcagtg ccaataccca gaacggaggg tacggcggca 1620
tgaacaccag caacaccaac ggctacggcg ccgccggcta cggcaccaac ggacaggcga 1680
gtttcaactc caaccaggct gcagcctttg tgcagaacag cttccagccc cagcagtttg 1740
cagcggctac tgccgtcgcc cagaacggcg tgggtcaccc ctcattcccc tacgcccagg 1800
cacaggcggc cgcccagcag atcgctccac catcaatggt gccgtacgcc atgccgcctc 1860
ctcagtttcc tcagtaattg aatcacatgc gtcaaaaaaa taacttattc cccccttttt 1920
ttggtctcaa gtactcaatc aataattctt tttgtattta catgacaggg ttacgacttc 1980
aaatttatca ggacttttca cgtctgcagt gtagcaaagt tgtttttttc caagtattta 2040
2040
<210> 44
<211> 990
<212> DNA
<213> Oryzias javanicus
<400> 44
aagcagtggt atcaacgcag agtggccatt acggccgggg gtattgtgga gtgacatgag 60
agggaggtca ctctgggtca tgtaactgag tggatagaga agaagctgga tcaggagttc 120
cagaaaatat tcgttatgcc aaacatggat gacatatggc tacccataat gcactcagcc 180
atggatacac gttcaaatgc caacttgatg actgcaacag atgatgcctt gaaggaaccg 240
gaaccagagg aatctgacat ccccgacatt tgaagactag aattttggag tgccgtgacc 300
aacgaacttc tcagacataa gaagacgaga gttgactcaa aagccaaggt gtgagaatgt 360
gtcgacaaac tctccaatat ttgattccat ctttgcagca tctgtgttgc aacagtccta 420
agaaatgtgg ttgaaaacag gagccctttt catttgtggc tacaaaaagc aatagatggt 480
gattaatgaa tggaagttaa ctttttagat tataaaaata aataaaaaaa cacctgatga 540
aaacaaaatt ttcacactgg atcagaaaat tgccatctga ttcagcttga ggaaccagaa 600
acctgtgatg cttatctgca gcaactcttg attgttgcaa actttttggt gcaatgcgta 660
gaactctaaa aaacctcact gatgaaaaag ccttgtatga ctaaaagggg ttgtccaaat 720
atttcaaaaa taagatccct agaatatagc agtcccagtg tggtcttttt atcaacccgg 780
catttatgca aacccagaaa tggcagttgg tccataaaat gtggcgtatc tgtcatggct 840
ccccatgttg ccatacataa ctatgaggct cggactgaac tggagtttca acaaaatttg 900
aaatcttctc tgcagctctg cctttccaac tctgttgaat gtatcagtgg gattgcagcg 960
tgctagctgt gcagaagctt gtttttcact 990
<210> 45
<211> 969
<212> DNA
<213> Oryzias javanicus
<400> 45
aagcagtggt atcaacgcag agtggccatt acggccgggg gcctcaggaa ggtgatggag 60
gagctggtgg gagatgaaca cttgaactgg gggagggttg tttccctttt tgcattcgtg 120
ggagtgctgg cgagacagct gagggggcaa acggacacga acccggggct ggacctcggg 180
cgggaagtgg cgcccggacc tgtgagctgc caggcgctgg cagaaactgt agctgatttc 240
ctgggaggag acaagaaaga gtggatgcta gaaaacgatg gatgggaagg cttttgcaag 300
ttctccagaa cagccagaga ggtgagccag gactcgtcca tgaagacggc gctctttgcg 360
gcggccagcg tgggcctagc cggactcacc ttcctgctgg tgcgctagaa atgtatcccc 420
ccgttctcca ctggggggca gtggctagaa caaccctcac ttgtttctta cgattgtaaa 480
gtttttcttc atctttgttt taccacagcg caggacccat tcaaattctt cactttaaag 540
gtttattctt caacagtgca gctaaaatac ttcatttgaa aatggaaatt tagttttaaa 600
atgatcggct gccttttttc gttcatgaat tgtgtataga ttaattctag tgtaaattaa 660
tatattatat ttatatcaaa tgaagcagtg accctttttt gtaatctgca gcttcgtccc 720
tcgttctgaa gatttttttg cttttttttt ttgtaggaat atctgtgatt tacgaggtca 780
gcaacacttt ccagtgagaa tggccacaca atggtgctat taaatgttgc atagtaagct 840
aataaaccag ggaccaatct gacttttgca tttaaacaat ctatccctcg acctatgaga 900
aatatatttt tacatttttt ggttctattt atttctccct attttgtgat aaaagaaaca 960
agtaagttt 969
<210> 46
<211> 2040
<212> DNA
<213> Oryzias javanicus
<400> 46
ggtgaaaata aggactgcga agtgtcccct gatgctgaca atacagatga tgggtccagt 60
aaggagcgca gggtggcttt taacataggt gactctgagg acaatgactg cagcgaagct 120
accaaacagt caaagaacct cagcaatggc tctacaaatg ctcaccaagt ccacttcaac 180
agtcatttaa atagcaaaga tgcacagatt ccaaacaacg gttacaccac ataccacaca 240
gtacacaagg actctggtct ctacaaagat ttgcttcaca agctccacct ggctaaagtt 300
ggagactgca tgggtgaagg tggcgatcgt cccattcgtc gcaataacag ctatacctca 360
tacactatgg ccatcatcgg gatgcacaca gacttcagac ataaagatgg agataaccgc 420
gctgctcaag acggggataa agcccaaggc ttgggtggcc ctgagaagaa gcgcgtgcgc 480
atggacagct acaccagcta ctgtaacgca gtagcagagg agacgactcc ggaaagtcac 540
caaaatgatg tgaaacttga aattgtagga gaagatggag gtagcagcca aagttctctg 600
gatgacaaac ttgaagagga caagccagag gtctccactc tgtttcagtt cctccagatt 660
ctgaccgctt gctttggatc ctttgcacac ggagggaatg atgtgagtaa tgccattgga 720
ccattggtgg ctctgtggtt ggtttacgac tcgggcaacg tgatgtcaag tcaacccaca 780
cccatatggc ttctgctgta cgggggtgtg ggcatctgtg ttggactctg ggtatggggt 840
cgcagagtca tccagactat gggcagggac ctcacaccca tcactccctc aagtggtttc 900
agcattgaac tggcctcagc cttgactgta gtggttgcct ctaacattgg tctgcccgtc 960
tctacaaccc actgcaaggt gggctcagtg gtggcagtag gctggctacg ctccagaaag 1020
gcagtggatt ggcgcttgtt tagaaatatc ttcatggcat ggtttgtgac tgtgcccatc 1080
tctggcctca tcagtgcagc tgtcatggcc attttcatct atggcatcct ataagcacgc 1140
agttgcatcc caatccagaa gggaggagct gctacttcaa tgcttaacca gatgaggagt 1200
aatgtttcat ggccaaaata tagtgctgtc ctcaatctga caacagaagg aaaaaaaaat 1260
gtggacgtgt ttaatcaaag tatgaaagta cttgtttcat gtgcctgttg ttcctggccg 1320
cgtcatttat tttcctgtta tatcactggt ttactttaat gtaagtagaa aagcttgcat 1380
atctgttcta tatcttgaaa acatgttttc attttttttc tccaaagact atatgcaatt 1440
ttctgtgtat aattagtttt acatgctggg gaacaatgct gtaattgttt tatttcaaat 1500
tgcaggtctt ttttttaatg ttttttttct tccattatca agattaagaa accttcccaa 1560
tttcttgtat ttgttatcaa gttttttgtc tttaataact ttgaagttta acagtcacat 1620
caaactgctg ctttggagaa ctgcagcttc ctttagcatt gtttagaatg tgctgtagac 1680
acaacttcat atgtcaaaat aaaccgaatc aaaggattgc tgtaattgaa gcactacaag 1740
gaaaagcgcc cttaaaggat aaaatgtcag tattttaaat gggaacagaa tttgttctca 1800
aattagctgc atatctgcaa cctttgacaa ttgctttcaa ccgtactggt tcaccctgta 1860
gcagccattt caactacagg aacttgcatc tttgaatgaa aaaaaaatgg catgttctaa 1920
ctttactctt taacccctaa tcttccaaat acaaaagaac tggaccttca aactggagtt 1980
ggatagtatg gaatggcttg cgttttcctt ttgcgtgccg ccagtgcgtg gacggttgat 2040
2040
<210> 47
<211> 943
<212> DNA
<213> Oryzias javanicus
<400> 47
aagcagtggt atcaacgcag agtggccatt acggccgggg gcgttgaaaa gcattaggtt 60
ttattgggta ggtgtttcta gcctagtagt tcgttaaatt tttaaagttt attctctatt 120
ctaaatcgtc gcttatcgac gtcaaagtca tgcgagcgat ctggttagtc gcggttgcgg 180
cgctgtctct gagtcccgcc gcagtgagcg gagccgacaa gaaaaagctg cagatcggga 240
tcaagaagcg cgtggacaac tgccccatca agtcccgaaa gggggacgtg ctgaacatgc 300
actacaccgg aaaactggag gacggcaccg agtttgacag cagcattccc cgcgacagac 360
ccttcacctt caccctgggc acggggcaag tgatcaaagg ctgggaccaa ggcctgctgg 420
gaatgtgcga gggggagaag aggaagctgg tcatccccgc tgaactgggt tacggagaca 480
ggggagctcc tccgaagatt cccggaggag ccacgctcat ctttgaggtg gaactgctca 540
gcatcgagag gagagctgag ctctgacgac gacaaggaag gaagcttaga ctagttttag 600
caggtgctgc tgttgcacta aggagaccat gatggaccct gacatacaag tcaaaacttc 660
tcagcagaac cacaactttt tattatgagc tgatgttttg gactcgtaac taaagaactg 720
tcggccagca gctgagttca tcttttgtag agaactgtcc tgcttttgag caaaaatatt 780
tcttaatttc aagaaattta aaagcaacct caaatacatg tacacccccc ccctttttaa 840
tagttaagtt tacattaaat caattaaaat gaatcagtgg gacaacagat taaaagcatc 900
aatgttcatt ttgttcctaa aataaaaaga tgacttgtgt ttc 943
<210> 48
<211> 930
<212> DNA
<213> Oryzias javanicus
<400> 48
agctgaaaga tgaggaggcc actctggtcc aggagctgga ggctgtggag gagcagagag 60
ccgctgtggc tcaggacttg acccaaagca ggatccagtc ccagcaactg gacacggagg 120
agctacagta ccagaaagaa tacagcgagt tcaaacgaca gcagctggag ttggacgacg 180
agctgaagag cgtcgacaac cagatgcgat actgtcagat ccagctggac cgactgaaga 240
agaccaacgt ttttaatgca acctttcaca tctggcacag cggccagttt ggtaccatca 300
acaacttccg actgggtcga cttcccagcg ttcccgtgga gtggaatgag atcaacgcag 360
cgtgggggca gacggtgctg ctgctgcacg ctctagccaa caaaatgggg ctgtgttttc 420
aaagataccg gcttgttcca tatggaaatc actcctactt agagtcactg acagacaaat 480
caaaggaact tcccctgtac tgctccggag gtctgaggtt cttctgggac aataaatttg 540
atcacgccat ggtggccttc ctggattgtg tccagcagtt caaagaagag gtggaaaagg 600
gcgacaccgg tttctgcctc ccgtatagga tggatgttga gaaaggaaag atagaggaca 660
cgggaggcag cggcggctcc tactccatca aaacccagtt caactctgag gagcagtgga 720
ccaaggccct caagtttatg cttaccaatc tgaaatgggg cctggcctgg gtcacctctc 780
aattctacaa caaataaaca gatattctgg atttaatcag ccctattata tacaaatatg 840
tgattctaaa agatgaataa aaaacttaat ataacgaaaa gaaaaaaaaa aaaaggccgc 900
ctcggccact ctgcgttgat accactgctt 930
<210> 49
<211> 922
<212> DNA
<213> Oryzias javanicus
<400> 49
aagcagtggt atcaacgcag agtggccatt acggccgggg gcagaagtcc tgacagcggt 60
taacgacagc taaattaaca gactgtaaat tcatgactgc acttacaaag aacaacgtcg 120
ttttctgctc actttgaccc cttcactatc aaaaataaga agcacgacaa agcagacatt 180
catggcaatg gagaggaaaa gtctgcctgt taggcgcgtt ctcaacaagc cagcgatatt 240
ccgcagtgac cctcggctgg atgcgactgc cagcctctcc aggggtggac gctccctcca 300
gcccaaagca cagcccagca ggcacaaaca tctgtatgac gtcaaccatc gggtcaagat 360
caagcctgat ttaaacacaa cactacccgt ccgacaaacc gcgtccatct ttaaacagcc 420
ggtgaccaaa gtaacaaacc accccaacaa caaggtgaag acagaccctc agaaggctgt 480
ggatcaacca aaacaattgt tctgggaaag gaagctgagc ggcttgaatg cattcgatat 540
agctgaggag ccggtgaaat ctatggatct ccccaaaggc ctgcagggtg ttgggcccgt 600
ttgttcagac agaacattgc tctctgccat cgccagctcc ctgcacacca gccccgttcc 660
catcaccggg cagctgaccg ctgctgtgga gaagaacccc ggtgtgtggc tgaacacggc 720
acagccgctc tgcaaagcct tcgtagtgac tgatgacgac atcaggaaac aggaagacct 780
ggtgcagagt gtgaggagac ggctggagga agctctgatg gctgactctc tggctaatgt 840
ggagtacgct gctcctgatg agtcagtgga gaaagaagag gagaagactg agcaggtgga 900
caaggagaat ttatagtcca gg 922
<210> 50
<211> 2198
<212> DNA
<213> Oryzias javanicus
<400> 50
attacggccg gggggagctc gcagaacggc ggcacggaag gaggccagat cgacgccagc 60
aaaggcgaag aggatgcggg aataatgttt gttggaggcc tcagctggga caccagcaag 120
aaagacctga aggattactt ctccaaattt ggcgaggtga cggattgtac catcaagatg 180
gatcagcaga cgggccggtc acgaggtttt ggctttattc tctttaaaga tgcagctagt 240
gtagacaagg ttcttgaaca aaaggaacac aggttagatg gtcggcagat tgaccccaaa 300
agagcgatgg ccatgaagaa ggagatagtc aaaaagatct ttgtgggagg ccttaaaccc 360
gacttgtcaa aggaggaaat tgaggagtat tttggagcct ttggggagat cgagactatt 420
gagcttccgc aggatccaaa gacagagaag aggaggggat ttgtattcat tacttacaaa 480
gatgagcctt cagtcaagaa ggttttggag aagaagtacc acactgtcgg tggaagcaag 540
tgtgaaataa aaatcgctca acccaaagag gtctacatgc agcagcagta tggcgcgcgc 600
ggatacggtg gacgtggaag ggggcgtgga ggccagggcc agaactggaa tcaaggctac 660
aacaactact ggaaccaagg atataacaac catggctatg gttatggaca gcagggctat 720
ggatatggcg gctatggcaa ctatgactac tctggttatt acggctatgg gggtggctac 780
gattacaacc agggcaatac aagctatggg aaaactccaa gacgtggagg ccaccagagt 840
agctacaagc catactgatc actcatagtg caggtctaaa gtaaataaaa agagatccat 900
ggtctgacca cagcgaacat gggctctggc ttttcctttg gagttggcag aaatttggac 960
tccatttgta cagatcaagc gaggaaactg gggaagcaat tgtcactttt ttttcccact 1020
ttttattttt ggttttcgtg tccatttaca tttccagcga tggaagtgtt atttttttac 1080
tgtacttttt ggtacctttt ttgaataatg tattgtatgg ttgtattttt acatgtcaac 1140
tggatttaca catgtggagg aacaagagct ttcaaagctc tcaaataaaa ttgtttagtt 1200
tttttttcct gtttttgtgt ttgtacaaaa aaaagcgttc ttcctcttgt attgaaagaa 1260
ttctgcaaga tttaagtaga aaaagaaggg ctttaacatg agcagaagtg ggtgtgtcac 1320
tttgttgcca atatcatgca gatggagtca ttccccccat tttctatttt ttgtcttggt 1380
ttttaaagtt tttctttcat gaacatgttt gcagttttaa ctggcttcac tggtgttatt 1440
caactgaatg caagcctgtt catgtaagag ggcattccct ctctcactgg agtttcattc 1500
cttaagtgtc ctggataaaa gtggtagaaa taaacttttg gttcaaatta caaaaacatc 1560
tttttgttat catttgtaaa gttaatggta aatgtatcta aatggcttgc taatttgtaa 1620
accttttccc cctggaggcc tctggaaagc attggcagag ggcctcagta tctgcagagt 1680
aactggaaat gttaatttcc atttattaca ccaacacttc tgctatagat aatggtaagt 1740
gattttcttt ggcattctgg gtaatggagt tttatgaaat aactgatcac tttaatgctt 1800
tttttttgcg ctttcatgat ttaaatgttt atttattggg agttctcaga gatgacatgc 1860
cttgttccat ggtgatgctg tgaattggtg ctgaagacct gtgaaagaac atttgagtga 1920
cagttcagtt ccaaaggagg gttctttgca gaacaatttg cttccactta gtgtgctctg 1980
gatgtaaact ttgctattgt acatgtggtt tttaacattc agcggaagaa gggaacgact 2040
gctctgcaag atgatgtcaa gctgcaagaa tgggtaacat ctctacctct gaactatgaa 2100
tgtgaccacc tgaaaatgtg atgcaattaa aagttttata caggttgagt aaaaaaaaaa 2160
aaaaaggccg cctcggccac tctgcgttga taccactg 2198
<210> 51
<211> 885
<212> DNA
<213> Oryzias javanicus
<400> 51
aagcagtggt atcaacgcag agtggccatt acggccgggg acttcacgcg ggtcgacatc 60
tcgacaggtc aagagctttc caagaggagg atatattaga ggaagggaag tcagtcatct 120
ttagaggacc cctcagtcac tgcagctgct gggaaccgca tcgatcatga ggatcgtcag 180
cataggccct gacccgtcgt tggcatatcg gccaaatttg atgcctgaag caaaagagaa 240
ggaagactac agaaacttta agactggaag tctgttcgac cgtgtctata acacgtacaa 300
actgatgcac acacaccaga cgctggattt tgtcaaacaa aagcactctg aatggagctg 360
ctgcagccgc actcagatga gcatgatgga agccatcatg tctctggacc agcttgttga 420
cgagtcggat ccggacgtgg acttcccaaa ctctttccac gcctttcaga cggctgaagg 480
catccggcag gcccacccgg acaaagactg gttccagctg gtgggtctga tccatgatgt 540
tgggaagatc ttagctctgt gggatgaacc acagtgggct gtggtgggcg acaccttccc 600
tgtgggctgc gagtttcaaa actcaatcgt gttcagatcc aactccttcc aggagaatcc 660
agatgacaaa aactctgcat acaggactaa atatggaatc tatgaaccaa actgtggact 720
tgacaacatc tacatgtcct ggggccacga tgaatatctt tacagagttc taaagaaaaa 780
tgaatgttct gtcccagaag aggggatgta catgatccgc taccattctt tctacccctg 840
gcattcacat ggagactaca tgcatctgtg caatgataag gacct 885
<210> 52
<211> 881
<212> DNA
<213> Oryzias javanicus
<400> 52
ggcggcggcg gcggcgggag ggggaggagc gtcctcggct gctgctccag aaggcggtcc 60
ggtctttgtg ggcgtgaacg tcccttcgtt cagaaaacga agcagcgagt tcctctctcg 120
ggccggctcc ggcccggttc aggcggacat gggctcgtag gtgtggaagt ggattccttt 180
ggagcaggtg atcttcatga cggcgccgtt cagagcggcg tccgtgatca gcctcatcat 240
cccgtcggcg atcagcgacg gctgcagaac tccaaacttg tccatggtgc gcttgaagtc 300
ttccttgaac ttgacgaact tgcccatgtt gtcctcctcc tccaccgaat gcagaagggg 360
cgtgtccacg aaggcggggc acagcacgtt gatgcggacg ccgtagttcc ccagaacgga 420
cgcgtccgcc atggcgcggg tgaagccgat gacgccgtgc ttggtggcgg tgtaaaccgg 480
ctggtgaggc gagtgcatga aggctgccat agaggacacg ttgatgattg tccctccctg 540
cttgccgtac tcccggctca tgtgctccag gcccaggtag gttcccttta tcaccgaggt 600
caggttgacg ccgatggtct tctcccagtt cttctcgttg ttgatgccgg cgttgttgat 660
gacgatgtcc agacgcccga acatgtctgc agttttctgg aaggcgtctc tcagagcgtc 720
tccgtcggtg acgtcacact ggatgaaggc gcagctgccg cctccgaact cggcgtccag 780
ctcggacttg caggcgtcgc cgcagtcctg gttgacgtcc accacggaga ccttcgcggc 840
gctccgcatc agcgcctcca ctctgcgttg ataccactgc t 881
<210> 53
<211> 860
<212> DNA
<213> Oryzias javanicus
<400> 53
ggccattacg gccgggggac cacagagctt agagaacgag aaaggtccac acaggtagtt 60
tgtccaccaa tgggcagtag taacatctag ctctttatca acatccagcc atccgcacct 120
ttcaaccgca tctggagcct gtcatcctcc atctgtgaaa ggcaaccatc ttccatcttt 180
gagcggtttt taccacctaa ggtcggcaaa aatggcagca attccatcaa gtggctctct 240
cattgccacc catgactact atagaagacg actcgggtcc aactccagca gcagctcctg 300
cggcagttcc gagtacaccg gggaggttat tccacaccat ccagcacttc cacggcaaga 360
ctcaggccac tggtggactt cctttttctt tgcaaaacag aaccaggcaa acggatcgga 420
caaccaaaag accggatcct acaccgtgac caacggtcag gtgtcctgca tcgccaggga 480
aatggttttg aaaagacagt tgagtgaaag cagcgaaagc ggaaaggtcg aaccaacaac 540
tccaccacca gtgtcctcct aggcccggct ccatcacccc ccacccccac cctcagcttg 600
ccattcatca catagtccag cagtggagat gaagatgacg atggcgatgt tgagggtgtt 660
ttgttacttt tagtaaatag tgtgcatgtc ctttttgttt tgtgcaatta tatgtatatt 720
gtgaccaaaa gtttaaaaaa atgtcaaaag tattttttca tgaacttcta ctttaaataa 780
taataaatag tgtctagtac aaaaaaaaaa aaaaaaaaaa aaaaggccgc ctcggccact 840
ctgcgttgat accactgctt 860
<210> 54
<211> 799
<212> DNA
<213> Oryzias javanicus
<400> 54
ggaacaaccc tgagtcctca ggtcatgttt cataacgcag cctccaacat cgtctgccag 60
cttctgtacg gaaaacgttt tgagtacgac gacgagatga tcaaagtgat tgttaaatgc 120
ttcactgaca acgccaagat tgccaatggg ccgtgggcga tgatgtatga ctctcttccc 180
atggttcgaa acctgccact gccgttcagg aacgccttta agaattatga gacatgtaaa 240
cagatcactc ttgatatcat gaataaacac aaggaaacta aagtccctgg agagccgaga 300
gactttgttg actgctatct ggatgagtta gagaagaaaa ccgcagattt gtcattttct 360
gaggatcgac tggctggcta ctctttggat ctttattttg ctggaacgga cagcacttcc 420
aacactttgc tgacaggttt cctttactta atgggccatc cacatataca agaaaggtgt 480
cagcaagaga tagacgtggt gctggagggc aaggatcggg tcagctttga ggacagaaac 540
aacatgcctt acatgcaggc tgtgatccat gaaatccaga ggattgccaa cgttgctcct 600
ctcagtgtct tccacgccac aataaaagat actgagctga tgggatattc tattcccaag 660
ggcaccatgg tcatccaaaa catgaggaca gtgctgatgg aggagggcca gtggaagttt 720
cctcatgaat tcaatcctga aaactttctc aacgacaagg gagagtttgt caaaccagag 780
gcctttattc ccttctcag 799
<210> 55
<211> 762
<212> DNA
<213> Oryzias javanicus
<400> 55
aagcagtggt atcaacgcag agtgggtggt gtacgaccac ggcaccggcc gcaacagccg 60
ggccgccgtc tcctacgaca gcctgaacca gcggatccga gtcctgcagc agcacaagaa 120
gcacactccg tgtcagaggt tttttgagta catctacttg tacaagagca tggtgatgtt 180
ccagatcgac cagaagacta aggactgctc caagatcgcg ctgacggcgg tctgggatcc 240
cttcaacatc ccagacaact ccaccttcga ggaccagtac ttcatcgggg gccccggaga 300
caacgtggag gtgcaggagt ggtcggacag gaagccggcg cgccagcatg agacctgggt 360
gggcgtctac acgcttaaag actgttaccc ggtgcaggag acctacacca aaaacagcac 420
ggtcaccacc tccacccgct tcatcaacct gcagctgggc atcagcgacc ccaacgtctt 480
ctccccgccc cccacctgtc aatcagcgcg gctccagagg atggacgagt ccgactgctg 540
agccttagat ccagtttgag tctttaggtt ttctctttac cttaaacact aacacaagct 600
tgaaactgac actggctcta gaatctgaac tgctgtgatt gatttaacag cttctgtaat 660
gtttgtccat aaaggattcc ttcagtctaa tgcttcttta ctcggggata gagctgttac 720
agcaagtgat gaaataaagg ttgatggttg aaaaaactaa aa 762
<210> 56
<211> 729
<212> DNA
<213> Oryzias javanicus
<400> 56
cgctcctacc cagtatctca tggaaactgc acacgacctc cgtttgcggt tgaaagcgta 60
catgcaacct cccaaaaaca agaaaggtga cacaaagccc ccggtcaagc cgtcccactg 120
caccatctac gtggccaaga gttatcctcc ctggcagcac agcgccttat ccctgcttgg 180
caagcattac aggagcaata atggagtcct tccagacaac aaagtgatag caggcgagtt 240
gggagccctg cctgagctga agaagtacat gaagagagtc atgccttttg tagccatgat 300
caaggagaac ttggagaaga atggacccag agtcttggat ctggagctgg agtttgatga 360
gagaaccgtt ctcatggaca acctggtcta cctgacgaat tctctggagc tggagcagat 420
cgatgtcgta tttgcaactg aggctgatga caaagtgaag gaagactgtt gccccgggaa 480
accgtttagt gttttcagat cagagcccgg agtgtgcgtg tccctggtga accctcagcc 540
ctccaacggc ttgttctcaa caaaggtcga catccgacag ggagatagca aagacaatgt 600
cgtccggcga ctagccaaag tcaacagact cattaaagat ttgtcccggg tgaagctcat 660
gaggtacgag gacccttgtt ttgggcctcg ccgggtgccc gtgctgggac aggaagaaaa 720
agacaaact 729
<210> 57
<211> 2122
<212> DNA
<213> Oryzias javanicus
<400> 57
ttgtctaact acgccactga gtcgacatgg cgggtaccgt ggcgaagaag tgtctcagcc 60
ctctgtccgt cctcaccagg aggctgtcag tgcccgagta catcagtcag tgctgttatc 120
accagaaagt ggtggatcat tacgagaacc cgaggaatgt tggttcgctg gacaaaaagt 180
ccaagaatgt tggaaccggc ttagtaggtg ctccagcctg tggagacgta atgaaattac 240
agattgaggt tgacagccag ggaaagattg tggatgccaa atttaaaacg tttggctgcg 300
gctcggccat tgcctccagc tctctggcca ccgagtgggt gaaaggcaaa tctgtggatg 360
aagctttgaa gatcaagaac acggacattg ctaaagagct tagccttcca cctgttaaac 420
tccactgctc tatgctcgca gaggacgcca tcaaggcagc ccttgctgac tatcgcctca 480
agcaaagcga cgaccagcag gaggcagcaa aggtcagcat ctgaagacgg actccttcag 540
caggacgtcc tcatgctaac agaagtagtg atgtcatctt acgttccagc agttacaata 600
gaatccagac ctcgtccctc tgcagcaggc tttcagcgtt agcctgcgac gtctgcttct 660
gtccactaac ctgtcacgta aacgcacaac ttatgcatcg ttcagtttca gtataaaact 720
gagccttata ttcttcagac cgaaggagcc gttggtccaa gaggaaaact tttgtaataa 780
acttgactga tatgagggga gggtcccaga acatggatgt attggtgcaa aagcttattg 840
gaaaaaactg caataaaagt attttaaact attgtttcag ttgcacattt gattggattt 900
ttcattttaa aacttgtgat ttcacatatc tttctcattt cacaaactga agttgtgctc 960
tgaccacaat ataaacatgt tctcaggtgt gctgattgtt ttattgtatc aaaagttaaa 1020
tatttctcag ttatctggca cagagtagtg ttttctcaca cttgtttgca tacagacgta 1080
attctagttt ttgaataaat atggcttata aataagtaca aaatatttct tttttgttgc 1140
gtttaaactt ttaaagttgc tcaaatttta tttggtgggt ctggtcttct gctgcagtga 1200
acaaaacgtt ccctcagaaa gccgttttct ctcctgcaag aagctggagc gtggcggagt 1260
cattgtgaat gtgcagagaa gctggccgca ggttcccgtc agctgggggc tcctgtgaat 1320
gctgttctcc gggatgcagc tccagctgtg gaccttctgc tgctccactg tcactgagct 1380
cacacggctc cttctcctca gggacacttg gaagctcctg gtccaggatg ctgctgtttg 1440
atttggagac gtcttcagaa gcggggtctg cttcctgttg gcagggctca cctttctctg 1500
tggcgtcggt agatttactg gcagctcgca ggctcttggc cgccctcatg atgtcggcct 1560
ggagctgctt gtgggagtcc accggcgtac tttcatgttc agatgcagct tttccctgca 1620
cttcgttaaa ggatctagct cctcccgttt tgtcccctgt cgtccacata catttttgaa 1680
gggaacgagg cggggtagta ggaattgact ttgcgcctgt ggctcattag aagcgccccg 1740
cagattatga ggaagaccag aaggaccagc gccgttatca ccaggatgag cagcacgttt 1800
ctctccagga agtccaacat gtctctgagg acatcctgct ctgctccggc agcttctgtc 1860
ggcgaggctc ggtgttcagt ggaagggact gtggtgggca tgaaggaatt cgagttcatg 1920
agttcctcct catctgtgct tccctccaga cgactataaa aagtggtggg cgtggccaag 1980
ctgcagctga cgcagagaac cacacacaga ctggccaaca ggagcatcgt atcattcagc 2040
gaggaggaag gcagcgtctg tgctgaaatc ccctttgact ttccccggcc gtaatggcca 2100
ctctgcgttg ataccactgc tt 2122
<210> 58
<211> 196
<212> DNA
<213> Oryzias javanicus
<400> 58
aagcagtggt atcaacgcag agtctctact gcaggccaaa aacgaggagg tggaggaatt 60
aaacaaagag ctcagacagt gtaacctgca gcagttcatt caacagacgg gagtgctgcc 120
gtctcatact cactcacgct cagacctgca ggagcagctg gaactaacac actatctgga 180
ggatggtttc caagac 196
<210> 59
<211> 701
<212> DNA
<213> Oryzias javanicus
<400> 59
cgttgttggc aaagtctaac ttaacactgg cgtggttgat tcctacaaag acggcgatgc 60
atctcatgac gctggcccac tccctcttga acttgtgggg ctctcccaga tgactgtcga 120
ggcaggggta cagcaggccg ataacagctg ctcctgtccc gcagcaaggt gggatccacc 180
aggcggagga gaacaacgtt gtcatcacct cctcgggaaa cagagtgacg tttctttgta 240
tctgtagtaa attgagcacc agagccagga agaccccgac cgtgaagagg accagacccc 300
ggcggatgag gttggtggtc cggacgtcgt gcagagaccc gtaagcgctc ccgagcaccg 360
tggttatgat ggacatcatc tcctcagcct tggatgcaaa ccagctcgcc ccagatgaat 420
gcttagcgtc agccttggga gaacaagagc agctccagca gtggtcctcc agtctcggca 480
tttggcactt tttcacttga tttctcttca gactgtgacc caaacttccg tcctgctcgt 540
tggcgcctcg gccagtgatc aaactatcgc tctgttttgt tttcccgcac tctacgacaa 600
ggactgcggt cacgttgttt gctcacatca tcgtttcagg aacaaaaaaa aagaaaagaa 660
agagaaaaca cacacacaca cacacagggt tgcttcattc c 701
<210> 60
<211> 697
<212> DNA
<213> Oryzias javanicus
<400> 60
aagcagtggt atcaacgcag agtggggggg tctgggagat gacggctctc tagagaacat 60
tatcacagct tttgacacag aggtggacca gaactcagtg aacccaggaa aacaaaggtt 120
tgaaaagctg accagtggaa tgtatttggg agaaatcgtc cggcaggtgc tactggatct 180
aaccagacga ggtcttttgt tcagaggaca catctcagag agtttgaaga aacccggaat 240
atttgaaact cgatatctgt cacagattga gagtgaccac ttagcgttac tgcaggtcag 300
gtgtattcta cagaagctgg ggctcgacag cacctgtcat gacagcatca ttgtcaaaca 360
ggtctgtgga gcagtgtcgc gccgtgcagc ccagctgtgc ggggctggga tggctgctgt 420
ggttgataag atcagagaga accgaggact ggaccatcta aacaccaccg taggggtgga 480
tggggcgctc tacaaacttc atcctcattt ctctcacatt ctccaagaca ctgtgaaaga 540
tctggctcct cagtgtgatg tgaccttctt tccatcagaa gaagggagcg ggaagggcgc 600
tgccctcatc gctgctgtag ctcggcagaa ccaggagatg tgacatggcg tgacatgaaa 660
acttcaaatc tgcaccttcc tgcacacgtc tgctctc 697
<210> 61
<211> 661
<212> DNA
<213> Oryzias javanicus
<400> 61
ttctttaagc tgtcctcaga actaccgcaa agtgtctgac tacacgtctg ttgtagcgga 60
tcagctgccc gaactacctg gagtgccaaa actctccact aagaatcact tattactacc 120
tcagcctcca gtccaacatc gtcattcccg ctcagatctt ccacatcgga ccatcccctg 180
cctattccgg agacaatgtt attgtcacga tcacccaggg aaacgaggaa aactacttca 240
gcacaagaaa actaaatgcc tacactgggg ccgtgtacct acagagacag gttcagggtc 300
caagggactt cctcatcact gtggagatga agctctggag acagggggcg ttcaccactt 360
tccaggctcg aatctacgtt ttcatcacag ccaacttgct ctaacatcct cagcgctgaa 420
ctgaagcgtg tcacgagaac atctagtcac taacgatgca tcttatcacc acttgtgctt 480
ttgaaacaag tctgtagcag atgcttttta aaccttgctg taaaagaaga ggacggatgc 540
ttcatgcaac ttcaggactc tatcaaagtg tatttttctc caagattgta cttaaaaaca 600
ttcatttgcc aaatttttgt aacccccaac gaaacataat tttgaaagta aaaaaaaaaa 660
a 661
<210> 62
<211> 649
<212> DNA
<213> Oryzias javanicus
<400> 62
aagcagtggt atcaacgcag agtggtggtg tccgacggga tcggcttacc gaacgcgctg 60
gcctacgact actccactga tcaggtctgc tgggccgatg caggtacgaa gcgtttagag 120
tgcgtatctc caaacggctc cggcagcaga gtgatccatc ccaacctgaa ctacccgttc 180
agcatggtct actacagaga ccacttctac tacacagact ggaggaggga cggcgtgatc 240
gcagtgaaca gagacagcag caagattacg gaggagtacc tacctgacca gcgctcacac 300
ctgtacggca tcgctgtagc aaccaaccac tgtctaccag ggagccacta accaccagcc 360
ggcagctatg gggggggcct atcggtgcca cagaggcagg agacggtcca ggaggagccg 420
ctcccctccc cccgctctga agatgtaacc caaaagttct gtacatttgt tttatacctc 480
atttctaaag tttttactgt atttttttta tattttgtat cgattaaaag ccacaatcac 540
ctaaagattg ttcaactctg aggccaaacg tcaccagaaa cggagaagaa ataaacagag 600
aaactgcctg aactcacctc tgcactaact tctcaccttt tttttgttg 649
<210> 63
<211> 624
<212> DNA
<213> Oryzias javanicus
<400> 63
cggcctgttg ctggaggacc cagtctgcta acggtgctgc tgggactcgt ctgcatggcc 60
gcgctgatgc tcccaacgct gggggagcaa gaatccacag tgcctgtcta cctccactta 120
agtgttaaca agaaacttgt tgctgcttat gttcttggtc ttcttacgat ggttatctta 180
cgaacatgaa gtgccgcaag gaggaggatc catgttttct tttttttaaa gaagtcttca 240
catcggagcc gctactgttg ttgaaccagt agagctgagt gtggaggagg ccgaggtctc 300
catcgttttc acaacgcgtc actacaaatg ttcgtacgat tttttttttt tttgatttcc 360
tgcagcaaaa gttgagctga tcctgagcga aggaatctac atgcagaaaa aaaaagctga 420
attaatgtac ataaaaccat gaagacgtca tagtgttgac tcgtaaccgt cattatgtgg 480
actcttccac gctgcagact gacagctgag tgcagttatt tgcacgtgtg gaaatatttt 540
aagaaaaaaa aaaaagcttt acgaatggat cgccattctt actatatcaa tgctccatca 600
tatgtttaat aaactttcag tcag 624
<210> 64
<211> 615
<212> DNA
<213> Oryzias javanicus
<400> 64
gagtcggaca ccattgccag taagtccttc agagacagtg ttttaaccga gtcctttgac 60
acggctaaag agcacatgtc agactcgagc agctccactg acgtttacca aaatccaacc 120
caaggaacgg actcgaaatt cgactccgaa cacagcacgc caagtcaaat attgttcgac 180
cacacaagcg atcgcacaga atgcgcagat gcgaggtaca ccgattctga tgttccagat 240
gaagaaaaca cgccggttga agagacggag ataaacgcag ctccgatcca gcaggagccg 300
gattctgttc aggagatcag ctgggatgat gttggcagct gcatagagga acacgcaatg 360
gagacctatg atacatgctg tgacaataca aacacaacag aatagtttct tatagcacct 420
taagttttcc tgtttttgta tcagatctta tggtcaacag acagacccct acgttccagc 480
cgcacggcaa cttttctatg acatgtaatg tcaataattg tgtgggagtc ttaattccct 540
taaaatgtag tcctaccgtc taatatatgt ggttatataa caacactcaa gcttgtacat 600
atgtttttta ctaat 615
<210> 65
<211> 1939
<212> DNA
<213> Oryzias javanicus
<400> 65
atttgccttg tggtttttat actttattgc tgcagttggt cttgttttct taaacttctg 60
tgaaagtcca tgtgtatttt taaacctaca ttttctattt acagctttta tcttttttta 120
aatgtttgta tttttagtgt gtgaccctaa agctctgtca tgtgtgcggt agacctgtga 180
ttgtagagat tccccacttt gctgctctgg gtcgaggtga ccgtgaactt gtggtcctga 240
ggagcgagaa tggctcagtc tggaaggagc atcgcaatcg ctatggcgat gaggtgctag 300
agacgatctt gaatgggatg gatgaggaac ttgaaagtca agaggaactc gggaagaaga 360
gaatccgccg catcatttct acggacttcc cgctatactt tgctgttgtg tctagagtac 420
agcaagaaag cgacctgatt ggtccagaag gaggttcgtt gacaagtaaa ctggttccga 480
tggtccaggc cacgtttccc gagacggcag tcaccaaacg tgttcgcctg gggctgcagg 540
tgagatggga caatgcagag gaaggagagg agagacagaa tcgttagtgc atgtgctgcc 600
tggaaaaaag catgttggat ttattttctg gttgatcatg gaacaatccg gtcataacag 660
catgggccaa ggttgtttga aattatttta tcctgttaca gaaaatcatg taacttttca 720
ttttaaattg tgcttgcatt tgaaccgctt atgtgctttt attttcctca ctaatgtatt 780
tatgtggtgt tcaacatgtt tattagaaat gtgcaagaaa aagttatatt ctgcatgttt 840
gtttcaacca gaaaatctag tttgttttca ttttccagca gtttgagtga tatttatctg 900
taatcaatgt actggcatgt attcagattt aaacaaagac aaaaaagggt aacatctgag 960
gacctgatct tgaaaccttt tttgtcatat ttactttttg tttccatgca cataagtgaa 1020
tgacttccat tatttgtatt tgtatttcat atattctcaa acataagcac caaagatgct 1080
tcagtaaaat aaaattccag aaagttcctt ttgaaagcca tatttgacat ccccgaccct 1140
gtctgaatca agaattagat aaaataactg taagatgaaa atacctacaa cattttttta 1200
agtaaatgca caactttgaa gaaaaaaaat catcttattt tcaagattgt taatttcaat 1260
ctaacttgaa gttaacgaat caaaatgtta cagaagggac atatttttgt cataaggttt 1320
cacaacttaa cagatatggc ttttaaaagt tttgtttgca tgtgaattgt tatattttct 1380
gctgcatgtt tgtcttttac ctttttaatg taaaaagtcc aaatggattc atcaaagcct 1440
ttaaaatatg tccgttttgg cagcttagtt ttctttcatt aagtggaaaa aaatatttgt 1500
ccagttaata ttttgtagaa taatataaca aataaatata gaataaactg accttccatt 1560
gtacgtttta atagctgtaa atgaagaaaa aacatttatt taaagatagt tgtgccttat 1620
aatagtactt ttgttgtaac ctcctaactg agcacactta atgaaaatta acatatttag 1680
atgatcttat attctttgat atatttattt taacaatttt tgtattttta ggtagcatgt 1740
atatttacta cagtgaacat gtctaactca gcagtggcct tgtggaatcc tcttagtgcc 1800
ttattatatt ttttgcaatt atgtttgtgt aagactgtca atagacactg taacacaata 1860
aaaataatta taaagtcaca caagaaaaaa aaaaaaaaaa aaaggccgcc tcggccactc 1920
tgcgttgata ccactgctt 1939
<210> 66
<211> 565
<212> DNA
<213> Oryzias javanicus
<400> 66
aagcagtggt atcaacgcag agtgccatta cggccgggga gatgatgcag gcagcgctgc 60
cggcgggact gaaacgatgg tgatccaggt gatcgtgaac acctttgagg gccagaagat 120
aaccgtggac ctgtgtcaag atgagaaaca gctgcagctg atgaccgtgc tgcaactgaa 180
gcagaaaata gcgctaaagc ttcccgggag agcagagcgg atggcggcag acatgcagct 240
gatcttcgcg gataagaggc tggacaagac agcaaactgc tgtgtgaatt cgggatcgtc 300
cacaagtctc tgatccagat ggtgatgtct ctggatggag gaagaggagc atgaccgcag 360
gaaaccagac atccagctgc acattcatca tgcatcaaac agactgttta cttgtttctt 420
ttatgcttta attgccttga tattgttgtc aaattctccc cagttggagt ttgttgcact 480
aataaaagtg gaaaacttag aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa 540
aaaaaaaaaa aaaaaaaaaa aaaaa 565
<210> 67
<211> 558
<212> DNA
<213> Oryzias javanicus
<400> 67
gtccttcact cagaggtttc tgtccagatc ttcatagaga actctctgat cttcatctgt 60
tctagagagt tcttcatgaa tcttcttcag ttcagatctg tctccagcag agggcgctgc 120
agtctatcat ctctgctgga taaacactgg tctcctagat agatagatag atagatagat 180
agatagatag atagacttta ttgtctgttc aatgaacaga aattcatctt tcaacacggc 240
ataagacaat aaattgattc aattgataaa aaagttaaaa agctaaaaag ttaataaata 300
tatagaaaat caaaagtcat agagatccta aaaaaaatca cataattcac catccacata 360
catacaattt aagtgctatt aaaaattaag cagagtgact gcattgggga taaaggattt 420
tttgtagatg tttgtcctgg ccaacggtgt tctgtatctc ctgcctgatg gcaacaggga 480
gaaagagtga tggagagggt gggtagtgtc cacagtgatg gaggtggctt tcctcatcac 540
tgctcgcctg tacaggtc 558
<210> 68
<211> 519
<212> DNA
<213> Oryzias javanicus
<400> 68
aagcagtggt atcaacgcag agtggccatt acggccgggg tctcttttga ggcaggaagt 60
acggaaaaca gtcaagatga aaattcagga gttttcactc aaggaaggac aggagctcaa 120
gatccgtgtc aagcccaacg atgactccgc tagatttgct atcaacatcg gcctcgatgc 180
tgacaacatc gcattgcact tcaacccacg ctttgactcg ggatcagacg tcaacaccat 240
tgtctgcaac tccaagtctg ggggaagctg gggtgaagag cagagggagg gacacttccc 300
ctttgcacgt ggagaggaat caaagtttta catcaacttc accatggagc aattttacat 360
caaacttccc gatgggagga tgatggactt tcccaaccgg ctgggagagg tcaagtacga 420
ctactttgaa gtcaaggggg atgcggtttt ccatggagtc aagatcaagt agaggctaag 480
cccctcctta accctggcct aaagcttgtt ctgtccatt 519
<210> 69
<211> 517
<212> DNA
<213> Oryzias javanicus
<400> 69
aagcagtggt atcaacgcag agtggccatt acggccgggg agagaacatc agcatcaaga 60
aagtctggaa ggtttttgtg gctccaaaat cctggcagca gatttaaaga tgaaagccgc 120
ttcgtcggta ccggttctgg tggtggttct ggctccgctg ctgaccggct cggatctcat 180
ccttcctctg gactgcagtg acatctacaa ccacaacaac agccaaccca gtggagttta 240
caccatctat cccatcggag ccacgtctgc tgtgcaggtg ttctgtgaca tggaaacact 300
cggaggacgc tggacggtgt tccagagcag actagacggc tccgtgaact tctacagacc 360
ctgggatcac tataagaccg gcttcggttc cgccgccaca gagttctggc tcggtctgga 420
gaacattttt aacctctgtt tgaggaagaa gtacgagctt ctggtcatca tggaggactt 480
tgagggaaac tcggcatacg cgtactattc ctccttc 517
<210> 70
<211> 485
<212> DNA
<213> Oryzias javanicus
<400> 70
gcagaaagat cgtagagtac acgtgccaca cggctttctt ttccagtatt gtgattgtcc 60
agtgggccga tctgatcatc tgcaagacca ggaggaactc cattgtgcag caaggaatga 120
aaaaccgcat cctcatcttt gggctctttg aggaaacagc tctggctgct ttcctgtcat 180
actgccctgg aatggacgtc gccctcagaa tgtaccccct caagcccact tggtggttct 240
gtgccttccc gtactccctc ctcatcttcc tttatgatga agccaggaga tacatcctca 300
gacgcaaccc aggcggttgg gtagaattgg aaacctacta ctgagtcagc acagaaggag 360
cttctaccac cgtttgctgc catgttacac atttgtttga gatgctgcat ctccgtgaag 420
cgcaggatgg agaagtcgag cataccttaa tttatgtgtc agtcacaata aaacatgtcc 480
gaacc 485
<210> 71
<211> 479
<212> DNA
<213> Oryzias javanicus
<400> 71
aagcagtggt atcaacgcag agtggccatt acggccggga gcagagaaca gttaatgaca 60
acaaggaaga ctgacttcgc gtgtatctgc ttttattcga aaccaggctt gcattaaatt 120
gcttcaaact ctacaactaa gtaaatttta aaagtgaaac attttgaggg tgagttttag 180
atggctgaat tgtcttcgag gcgtctgtag ctgtgctgcc tcggattgtt gacaaagtat 240
tagctagctc agctggccct cagaagctca tcgatatgcc tgctcgaaac atcctgtcca 300
acgatttttc aaccagcaga gtgaagtact ccaggttagc ttctgacgat gacggctaca 360
ttgatttaca gtttaaaagg agcccgccta aagtaccata caaagctata gctctcgcca 420
cagtgttatt cttgatcggg tctctcttaa tcatcatcgg tgctcttctc ctcgctggc 479
<210> 72
<211> 468
<212> DNA
<213> Oryzias javanicus
<400> 72
ctctgcacat aggctgaagg ctatggaccc cagttgttag ctgcggtgcc ccctagtgcc 60
tctgtcccag accagccgct tggtgagccg cctgattgaa atcctgccag aaaccacgcc 120
cagtactctc tggccttaca cctcctggag gctgtggacg tcctcatcac ggaggaggac 180
tgaagagccg ggtcctggtc tgttatacag tcatcctcat catgtctctg tcatagaagc 240
tgcttttaaa ggtttacttc atcaggttga gtgcatgaat cagaatctgg ttgaatctgt 300
gggttaagca gagcgttgtc atagaagact tggtcctctc gtgccacatg agtttagttt 360
ctctgctctc tgttagtcaa catgtaaata aagaagagcc ttatttttac ctcgcctgtc 420
cctctatgcc caactgcttt cactaagaaa taaagaggct atgagaac 468
<210> 73
<211> 461
<212> DNA
<213> Oryzias javanicus
<400> 73
cccattgcaa gaggggcaat ggtctgatca aggtgaacgg cagacccctg gagatggtgg 60
agcctgctac acttcagtac aagcttctgg agccagttct gttgttggga aaggagcgtt 120
ttgccggagt tgacatcaga gtcagagtga agggcggtgg tcatgtcgca caaatctacg 180
ccatccgtca ggccatctcc aaggctctgg ttgcatacta ccagaagtat gttgatgaag 240
cctccaagaa ggagatcaaa gacatcctga tccagtacga ccggaccctg ctggtcgctg 300
atcctcgtcg ttgcgagtcc aagaagttcg gtggacctgg agctcgtgcc cgctaccaga 360
aatcctaccg ttaatcgctc tgtacattta tacaataaac tggatgaaag gaagaaaaaa 420
aaaaaggccg cctcggccac tctgcgttga taccactgct t 461
<210> 74
<211> 1262
<212> DNA
<213> Oryzias javanicus
<400> 74
gcagtggtat caacgcagag tggccgaggc ggcctttttt ttctttgaga tgcaaaactt 60
ttatttgaat caaacaatat caattttact tttgtttatg tgaacttttg aagaattaaa 120
accacaaaat gtaacaaact acaatagatg attttgcggt ttcagtgttt gtgcatcgtc 180
ttcttcgctg ctcgtctccc ctctagttct ggccttggag ggcttctgcc atgaaaagct 240
tcccgaggtt ctgcgaggtg aattccttca cattctggca gtagttatta atttgccctg 300
caataagcag cgtgtccatg cgtggaggcg gctgaggggg cttgaacaat ttgttgatgt 360
cctcctcagg cagcgcggcc tctccccggc tctgtcgctg ggcgttctcc tgctgccgcc 420
tctggagata ctgatgcttc tgctgctgct gcttactcag gttgcgactg taggtgttgt 480
atttgacaat ttcctggctc atgtcatcca ctctgtccat gagcagctga aggctcttct 540
ccaagtgatt gctgctggag aggttgagca gttcgtgctt gtcggcaacg gtggacttat 600
cctccagctc ccacatcagc acgctgatga ggtgggagtt tttgatgacg atgggcacct 660
cctcaaacat gttctcaaat ccaatgctgg ccttcttcaa gccctctggt gtgaaatctt 720
tctctttgca gatctccatg agtttggggg tgagccggta ggctttgaga gacagggagc 780
cttgggctgt tttgatggga tcgtagatga gaacgaccga ttcctcgatg gcatgctggt 840
agctgaactg agagtccagt agagctctgc tgacaaacga gccatagagg gtggactggt 900
accagcctac atgcagatgg tcaatgttga cgtgacgcag ggaacgcatc atctccatct 960
ggtactggac ttcgtcgaag tctgcatcgt cttcagtgtg ctgggggaag gggaagcagt 1020
tggtgatctc cagccggtcg tctaccacca gacccagcag gacgccctga accacttcgc 1080
tgccctggcc ctcttcctgg tagtgcttaa ttattttcag caccaccaat ccctcgatct 1140
ggacctgctt gactggagag tccagcgtgc cgccagtaga agccattcca gaacctgatg 1200
ccgagctgag ttctttacgc gaagccatga gtccctcaca ccaaaaaagg aaacattaag 1260
gg 1262
<210> 75
<211> 1793
<212> DNA
<213> Oryzias javanicus
<400> 75
aagcagtggt atcaacgcag agtggccatt acggccgggg agagcggaga gtggctcctc 60
aagactcgtc caactccttt gttgcgtagt ctctacgttt acacttcggt tcgtgttcgg 120
tccggtccag gcatcccagt gtcgtggaac ccaccccgca tgcgcgctgt gttagcgtta 180
atttaaaccc ggagttacat tcgaggggaa atggtcgcgc agaggatcct ccgtgctgtc 240
atcatgggac ccccgggttc ggggaagggg acggtgtctg cacgcatcgt caaaagtttt 300
ggagtgaagc acatctccag cggggacatt ttgagggcaa acatcaaaca acaaacagag 360
ctgggcttgt tggtgaggtc ctgtatggat cagggtcagc tggtccctga tggactcatg 420
tcccgtctca tactgaaaga cctgaaatcc atggagcaca tcagctggct gctggatggg 480
ttccctcgca cagtaagcca ggcggaggcc cttgatcaag cgtacactct ggacacagtc 540
atcaatctga acgtaccttt ccagaccatc aaagaacgcc tgacctctcg atggactcat 600
atttcaagcg gcagagtcta caacactgat ttcaaccctc ctaaagtccc tggttttgac 660
gacgtaactg gtgaacctct ggtccagagg gacgatgaca aaccggacac agtcacacag 720
agactgaagg gctatgaaac ccaaacacag cctgttttgg agtactacag aagtaaaggc 780
gtgctgaaga ccttcactgg gacggaaacc aacaagattt ggcctcatgt ggaagagttc 840
ctccatcgaa gattctcttt ggaaagtcag agggcagctt aacgaagaaa agcttgaagt 900
ttacctgtag gaacctgcgc ataaaatgtc tattgaacgt ttctctgaat gttccacatg 960
aatttgtttt gagttaaatt taagttagta ctattaagtt agtttattaa cacttttgtt 1020
ttttattcat tccatatggt gccttttgaa ggggttatca gggtttggca caagcttttt 1080
tattgaatta atactctgat tcactcaagc agcatcacct tcagcagcgc gagtgtaaag 1140
gtcgattcca gcatccctcc acttttcact cgagctaaat caacaaactt aaccgccttt 1200
tccatgaaga atccatccag agtaatatta tggtgagcgt tttttttttt tttagacttg 1260
cattagtgtc ttaaaggatc ttgtatgtgg agcggccgac tgcccccaaa agggaaatgt 1320
gatgtgcagt tgtatgcaca taagtgcctt ttgctgcttc tttagaccaa gtagctcagc 1380
aaatgtgatt tctcaacctc tccattgatt aaaagtaaca ttcctatttg tgacatttgt 1440
tgtatttttc ttatcttagt ggttttgctg gtgaacttgt tgtagaaaac aggtgtttat 1500
gtttgagatc tttacttact gcattaataa ttaaaatcag actgtattca tattttttta 1560
agtatatggg tgagttagca cttagctcag tgtttaaacc tgttaaaagc cttgaaagag 1620
ctaatctgca gtgaactgta atgatttctg atttaaagcc taatgttaag cagaatatgg 1680
tcactccatt tccatctgtg ccaccagtat ttatgaaata ataaatgtct taaatacaaa 1740
aaaaaaaaaa aaaaaaaggc cgcctcggcc actctgcgtt gataccactg ctt 1793
<210> 76
<211> 1791
<212> DNA
<213> Oryzias javanicus
<400> 76
aaaaaaaaca atttatccag tccacaacag tttcagcttt aaaagctgga gcaactaaaa 60
aagatgaaaa acataaataa atacacacaa cagaataagt acagaagaaa ctgtcaggaa 120
aaagtctgtg gatttctcca tttgcacaga aaactactta ataatgaaaa taataacgac 180
acatgattct taaaacagtc tgtaaaggaa ccaaaacatc acagaactca gatttggctt 240
ttcagtgcaa aaacatccag tttgtcccat aaaattagat acattattaa acattgtgat 300
tggattcgct gtaagctgat ttcattttga actgatccgg agtgcctctg cttgttctat 360
gaggtaaagc aaagtcgaaa catcacagtc tggggtgttg ttacatcagc tacagcaagt 420
tcctgtccgt ctgtcagtcc aagctctttt aaagttttag acagatttgc tcgtgtcctc 480
tgttcaattg aaggtacaga ctgtaaatat aatgttttgt tttttccctc cactgtagct 540
gttatagcag gtgacttcat ctgtaatgat gcactctcag tcagaaagtc taacacttcc 600
tgcagtttgg aagatggatg aaagtggaga tcctgaggta cctgtctgca agcgctgcag 660
ttttccttcc tttctgcctc aaaggtgtag gtatataggc catcaacgtc gttgaaaacc 720
atatagttac tgagaggtac ataggcactt gttgctaatt tgaaaacttc tgtggcacaa 780
gcagcagcaa tgactgcatt tgttgatgca acagctggga ttatcctttt aacaacaccc 840
tgtgtaagtc tatacgagac tcctgttata ctgaattctg ctgccctctc cagagatctc 900
tggaaaaccc actggatgtg ctctgggtca tctccatcca atgccacacc atcgccaaac 960
ggtagctctt tgggccactg cagtattctg acatattcaa tgcaatgctc tggcaaacga 1020
ggcattgaag ctattgtgca catagggaag ttgatctgag gagggtaaag ctcaagggtg 1080
cagtcaatgc aggccgtcat accaggcaaa atgactctgg cgttgccctt aaagccttca 1140
gtcccaccat cgattaatgg gacaatggac cctggatcca acacgccgtc ctcatacacc 1200
agcatagaca gcagcatacc gttcatccac ctcctggcaa caatagaatc aagtccacaa 1260
acaattatat ggaattgtct gtagaatgtt tcgtcaaggt cctgaatttt ttaaaatgag 1320
gaacaacaca gcatcccgga acacgactgt tgatgaaatc cgctgccact tcagctttag 1380
gtcggccaac gtccttaggc ctgaagagaa actgacgatt cagattggag acatcaatgg 1440
tgtccatgtc aacaacgctg atgttacgaa agcccgataa agccagatct ttcagtagct 1500
cacatcccag cccgccagca ccgatgacca ggattttgca ggtttccaac aagaactgta 1560
aactctctgt gctgggttcg aagtccggat gtgtaaatgg tcctggcctc tccaggaatg 1620
tttttatgtg gttccatctg cctccccact cacaggaaac tccagatcct ccatctacca 1680
ccatgttctc agtcagctcc actactttcg ttcttttctt ctccggctca tccatctccg 1740
ccatgttgga gtccccggcc gtaatggcca ctctgcgttg ataccactgc t 1791
<210> 77
<211> 434
<212> DNA
<213> Oryzias javanicus
<400> 77
aagcagtggt atcaacgcag agtgccatta cggccgagat acagacgagc gcgacctgat 60
caacatgggg aagatctacc aggtggttgt tcacggcctg aggggggaga agatgatcat 120
cgatctgtgt aacaccgagg agcagatgca gaagatgacg gttctgcagc tgaaggagaa 180
gatagcgcag agactccccg agagcgcagg agaggaatct ctgcgtctga tcttcaccga 240
taagatgttg gatgaagact ccaagctgct gtccgaatat ggaatccaga acttgtccgt 300
tatccacatg gtgattaaag ttcctggagg actgcaaatg tcaccacaac cgtctgccct 360
ctgaacggct gctgctgcct tcatctgcca tgttctggac ttttattggt tcactggttc 420
tgtttctttg cctg 434
<210> 78
<211> 427
<212> DNA
<213> Oryzias javanicus
<400> 78
gcctttctcc ttgatgacct gagggaaaag cttgccgttg tctgccttct ggtagagggt 60
ctcgtggaaa aaaatgaccc caccaatgtt gttggcgatc gacggatcgc tggagaaaag 120
aatgtcacgg aagcaacggc ggttctcctc tgtgttctcc accttgatct tctgcaggcg 180
tttagccatg gttcctgttg attcatctgc agccaggatt ccctttcctg aagcaacgat 240
tctctgagca atgtcagaga gctccttctt cttctctgga gacaggtatg gatactgatg 300
ggtcatcttg agggttgttt tttcaacgag gacacgtgaa gtgacacacg gagctgcaca 360
gcttcccgac aggaatgatg aagaagcccc ggccgtaatg gccactctgc gttgatacca 420
ctgcttt 427
<210> 79
<211> 1774
<212> DNA
<213> Oryzias javanicus
<400> 79
agagcttgtt cccgggtttc ccttggacaa agcagagagc ctctcacagg agctggagaa 60
cgagatctgc cagaacatcc tgctgttgtg ccttagagag ctctttgagt tcagatacat 120
gcagaccgac ccgaactggt ccaacttctt ctacgatcct cagactcacc gggtggcgct 180
gctggacttt ggagccacaa gaggttttga tcaaagtttc accgacatct acatcgagat 240
tatcagttca gctgctcagg gcaacagaga aggagttctg aagaaatcta tcgacatgaa 300
gttcctcact ggatatgagt ccaaggccat gacgaacgcc catgtcgacg ccgtgatgat 360
cctcggcgag gcctttgcct ccgccgagat cttcggcttc ggctctcagt ccaccaccga 420
gaggatccac aacctcattc cagtcatgct caaacaccgg ctcacgcctc cgccggagga 480
gacgtactct ctgcaccgca agatgggcgg ctccttcctc atctgctccc gcttgaatgc 540
aaagctgagc tgcaaggata tgtttgaaga cgcctatcgg aagtactggg aaggccgcaa 600
gcccaccccc taatgaaatc aagccaactg atggactagt actgggggaa gagtggaagc 660
catgtttgtc tgagatattg aacactggtc tttactgtta tggacaaata cgtgtgaggt 720
ttaaagcata taatattcag aggaatcgtt tttttttatc aagaggaaac atgtcatctg 780
cattgttttc agctaaacgc acaggaacac atggctccag aagaaaaaca tgaatgtaaa 840
gaggttgtat ttttctgatc atttcaagac gatatgcaat tttttttttg tcatttcctg 900
tttggatcgc tttgctgttt taactcttta gctaaactgt ccaagctact ttttattttt 960
aaaatgaaga gtttatgagc gtaaacagtt tgagggaagt gcgcgtctcg gtataaacca 1020
ggaccaacgt gtctgaatca caatggaggc gatcgaatca cctcgtgttg aacaaacggg 1080
ctgatgggaa ttcttatttt tgctgcggaa gtctgactcc taatgtgtaa atgtagatta 1140
tagacttctg cgttatttta gataatgagt gtacagattg atcaatagct gttaattaat 1200
tttatctttc gtttctgtcg tttttgtttc aaactgttac tttagttggg taaatcctca 1260
aagatttctg ggtaaaagca tcaattcact cagtttgttt caccagataa gttaatttcc 1320
aatttagggt tatggaaata accctttaag ctatcatgga ccacataaat attgagttcc 1380
agtaaaacaa aaggatgaag tcagaagatt tctatgtttt tgtagttctg agtaaatata 1440
atactttgat ccataatggt ccatgtcaca tcaacacatc attttcaaca catacttcag 1500
ggaatgaaaa aactcaacgc acaagatcgg cttgtggatt tacctcgcgc tcccacgccg 1560
tttctgtcct tataacttcc cctccacagc ctgcctcctg gtcggtgtgc aactctaaag 1620
ttctcaagac tcttttacct gccgtttggt gtaaaatgtg ggagaagaaa cttaaaaaaa 1680
caaaaaatgt gcctattgtt tccagctgag agttttgagt agaaatgttg actttctatt 1740
aactgaaagt tggaataaaa tatcatgtga atag 1774
<210> 80
<211> 1759
<212> DNA
<213> Oryzias javanicus
<400> 80
ggagaagcgg tgcaccttgc cagggatttc ggttatgtat gcgagaccga gtttccagcc 60
aaggcggtag ccgaatatgt aaaccgtcag cattccgacc caaacgagca agtccaaaga 120
aaaaacatgc tattggccac aaaacaagtg tgcaaagagt tcacagacct gctgtcccag 180
gaccgctcgc cgctgggaaa ctcacgacca cagcccattc ttgaaccggg aatccaaagc 240
tgcttgaccc acttcagtct aatctcgcac ggttttggga ctcctgcact gtgcgcggcc 300
gtcacggccc tgcagaacta tctgaccgag gctatcaaag ccatggacaa aatgtacctc 360
aacaacaacc ccaatagtca ctcagataac ggcactaaag gtggagacaa agacgagaag 420
cacagaaagt gatgttttgc atcccaccgt caacgaggct caagcgccct tttctgcagc 480
tgcctcaccc ctctaacctc cccaatcttc caatataaat attataaata ccctataaat 540
attattgata aagtcaaacg tgccacttcc tggtcttacg ccggttttac gtgatttgtt 600
tgtacatcct tcggagccaa gaggaaagga cgcaaaaaat cctcttcttc catctgaacc 660
atacaagaca ttgttttaaa aaatacaaaa aggaaaaaaa agaagaaaaa aataaagctg 720
cagaggagca gctctaggta aaggtgtact gcctcaccca gaggactttt tatgaactct 780
cccccttatt ttttatgagc tgcaacgaat ggactgaaac ccagcgaccc ttctatatct 840
caattttacg attgtggcaa aaaagaagct aaaagtggac gaattcttat cagtattgtg 900
agtaaacttg aaaaaataat aaaaaaaaag aaaatctcca gatatccatg ccatgacttc 960
agttgtagac tctctccaag cgaccaactt gaactttttt tgaatttcaa cacgattcgc 1020
ggttatataa gggaatcagt gttcatgtat gtatatattt atttatgtgt aatttaatgg 1080
gaattgtaaa tatggtgcgt ctgttgaaac ttttttttta tttatctggt gcctcctgtt 1140
ttagtgggtt tgaatattcg gttagttctt cacgtggttt tatggttcta gaaaagtttt 1200
tggggtcttt tatttctctg ggatatttcc aattaagccc tcttgtagca tgttgacgca 1260
acactgaagc aagtgaacaa aaattgaata gaaataaact ccagtttctc tctctctata 1320
tatatcaccc tattctgtta ttttttgtta ttatttgata tgagacagag cataagtgac 1380
accctgagaa gattttgttt tgtgtctatt ttttatacaa gcttatatgt gttgtgccaa 1440
tcagaaaacc tcttgtcctc tccttgaagc accataaaag caataaatgg aatctttttt 1500
tctctctctc tcaatgttcg aagacattca gaaacatttt ttttgggacc acctgatatc 1560
ttgttatgtc cgattatgtc caaaaattgg aggcggtttt agctgtattg tatagacatg 1620
tgctggcaat agttcctatg cctgtcaatg tattatagtc ctttgttgcc cagaaaaaaa 1680
taaatatttg atacgcttaa aagtaaaaaa aanaaaaaaa aaaggccgcc tcggccactc 1740
tgcgttgata ccactgctt 1759
<210> 81
<211> 368
<212> DNA
<213> Oryzias javanicus
<400> 81
aagcagtggt atcaacgcag agtggccatt acggccgggg actcgcgagg cgtggtcagt 60
cacagcggac aggtgcggcc gaagctcctc tctcacctct gcaggaacac acacacacac 120
aaacgcagca aaaatgccaa agtgtccccg ttgcgctaag gaggtttatt ttgccgagag 180
ggtgagttcc ttgggaaagg attggcacag gccctgccta aaatgcgaaa agtgctccaa 240
gaccctgtca gctggctcac acgcagagca tgaaggtaaa ccttattgca ataatccctg 300
ctatgctgct ttgttcggac ctaaaggatt tggacgcggt ggcgctgaaa gccacaccta 360
taaataga 368
<210> 82
<211> 351
<212> DNA
<213> Oryzias javanicus
<400> 82
aagcagtggt atcaacgcag agtggcacca atcagcgtca gggtccagag tgagatcggt 60
cagagagccc agcaagtgaa ggaaatggca agcccctacg ttgaacagat gagggaaagg 120
ctggacccct acgcccagga cctccaggct cgtctcacca gcctctatga ctccttcgtc 180
aagtccaact aaatccatct ccagcttacc ctacatacaa agaaactgca cctaccatca 240
aacagctgtc tgacatttca gacaaatgtc ataaatagca aatatgtatt ggtctgtgac 300
ctcactctgt tctctgtcta aaatatgaaa ataaactaaa agcaaacacc g 351
<210> 83
<211> 517
<212> DNA
<213> Oryzias javanicus
<400> 83
aagcagtggt atcaacgcag agtggccatt acggccgggg agagaacatc agcatcaaga 60
aagtctggaa ggtttttgtg gctccaaaat cctggcagca gatttaaaga tgaaagccgc 120
ttcgtcggta ccggttctgg tggtggttct ggctccgctg ctgaccggct cggatctcat 180
ccttcctctg gactgcagtg acatctacaa ccacaacaac agccaaccca gtggagttta 240
caccatctat cccatcggag ccacgtctgc tgtgcaggtg ttctgtgaca tggaaacact 300
cggaggacgc tggacggtgt tccagagcag actagacggc tccgtgaact tctacagacc 360
ctgggatcac tataagaccg gcttcggttc cgccgccaca gagttctggc tcggtctgga 420
gaacattttt aacctctgtt tgaggaagaa gtacgagctt ctggtcatca tggaggactt 480
tgagggaaac tcggcatacg cgtactattc ctccttc 517
<210> 84
<211> 314
<212> DNA
<213> Oryzias javanicus
<400> 84
taagcagtgg tatcaacgca gagtggccat tacggccggg gcccctcagt gcactggaac 60
ccttcatgga ttatactctg gccaattcca tcttcctttg aaaattaaca atttaagacc 120
gaaaggaacg ctaaatttaa gctaaaatat tagaaggatg gtgggattta aaccctcaga 180
tgttcctcct cctctggggg tgaagatgat gagcgctgga gcagcagctt gtgtggcaga 240
catcgtcaca tttcctctgg acacagccaa agtccggtta cagattcagg gtgagaagaa 300
agcagtggaa ggca 314
<210> 85
<211> 242
<212> DNA
<213> Oryzias javanicus
<400> 85
cacacctgga gtacacgccc aggtctggag agaagatctc ctgtaaggtg gaacacgcca 60
gtctgaagga acctctcatc actgactggg acccgtccat gccagaatca gagaagaaca 120
aactcgccat cggagcttca ggtctgagtc tgggtctggt tctgtctctg gctggattca 180
tctactacag gaggaaggcc cgaggtcgta ttctggttcc cagcagctga tgctgcagac 240
ag 242
<210> 86
<211> 187
<212> DNA
<213> Oryzias javanicus
<400> 86
aagcagtggt atcaacgcag agtgccatta cggccgggga cgcacagcaa gtggaacaaa 60
ccccagccaa aatggtcaaa tggtcagact ttgagcgtgc caccatccag gacatcttca 120
ccaagattga taaagatgtc gtagggccgg cggctctttc aagatgtctg atcgtctacc 180
cctggac 187
<210> 87
<211> 199
<212> DNA
<213> Oryzias javanicus
<400> 87
ccatccgtgt ctctcctcca gaagtcctcc tcctctccaa tcagctgtca tgctacaggt 60
ttctatcctg ccagagctga actgctgtgg aggaaagatg gagaggagat ccatgaagga 120
gtggagaaag gacagatcct ccccaacaat gatggaactt tccagatgag cgttgatctc 180
caacttccat ctggagaag 199
<210> 88
<211> 1649
<212> DNA
<213> Oryzias javanicus
<400> 88
ggcggatcct tcactccgaa ccccgctcac agtcagcagc cgcgggaagt tcacgcgcga 60
cggcttcagt cgggtggtcc gagcggtgca gcgacagtag ctgttgaatc gagtcccgct 120
gggagatgga gcggtggaaa ggcagagtgg cccttgtgac cggggcctcg gtgggaatcg 180
gagcggctgt tgcccgagcg ctggtccagc aggggatgaa ggttgtcggc tgcgccagga 240
atgtggacaa aatagagaaa ctgtcagcag agtgtcagag tgcaggctac agcggcacat 300
tgatccccta caagtgcgat ctttccaacg aggaggagat cctgtccatg ttctcggcga 360
tcaagacgct gcacaagggc gtggacgtgt gcataaacaa cgccgggctg gcgcacaatg 420
aacccctgct cagcgggaag acagagggct ggaggaacat gattgatgta aatgtcttag 480
ccttgtgtat ctgcacccgc gaggcctaca agtcaatgaa ggagaggaac gttgacgacg 540
gccacatcat taacgttaac agcatgggag ggcatcgagt tgtgccaagt gctgacgagc 600
atttctactg tgccactaaa tacgctgtga ccgctctgac cgaaggaatc cgacaagaac 660
ttcgagaggc aaacactcac gtcagagcaa catgtatttc tcctggagta gtagagactg 720
agttcgcctt ccggcatcat aacagtgatc cagagaaagc agctggagtc tacgagagca 780
tgaaatgttt aaaagcagag gacatcgcga gtgcggtcat atttgtcctc agcgcacctt 840
cccacgttca gatcggagac gtgcagatga ggccggtaga gcaggtatca tagcagtgac 900
tatgaagccg gcagacagca gccgcagaga cttcttggcg acctctgctg gagagcgagc 960
tcggaggaag ggggttcgac ataaatcgac cgcgggtggt tctgcagggg caagtgcagc 1020
tgaagcctta aagcggccgt tcaaggaaac gataactctc taaatcacta ctgaaatact 1080
actgaaagca gcagccctct ccagcgctga gctgagcccc tctgacgtag gaacaaacag 1140
ggagagctgc tgctgcgctg cgtcctcctc tgcacaccca tcgcctcaga gggaaaagat 1200
gtcaatgatt gcacattatg ttttgttttt gtaggtcata caacatatct ttttgaattg 1260
taattaaaaa aataattact gtttataaga actgttcctt ttttatgaca tattttggat 1320
tctaccaaca cttagttttg gtttcttatt gactaatctt agtcgatact gtcataccca 1380
ttgtaaccat aacagccctg cctttccact cactataatt tcacagaaac agtcatccct 1440
tcttattttt gtttgattag gagtaaagcg caggtttctc ctgtcagcct ttttgccctg 1500
tctattatta gctttctgca ctttggattc atttgtgagg tgaaggaaaa ctgcgggtga 1560
ggagactaac tgcacatgtg tacctgaaac ataacccctg tttaaatttc tcataaattt 1620
aattaataaa tggtttccca gatccttca 1649
<210> 89
<211> 151
<212> DNA
<213> Oryzias javanicus
<400> 89
ggaacgccgc ccgggatgct ttctgcctcc gggccaagat caatagcctg gcttccaaag 60
gagagtacaa gccctcaggt gcagccgacc agtcgtccat gcagtctctc cacacttcca 120
actacgtcta ttaaactcat tgaacactta t 151
<210> 90
<211> 187
<212> DNA
<213> Oryzias javanicus
<400> 90
aagcagtggt atcaacgcag agtgccatta cggccgggga cgcacagcaa gtggaacaaa 60
ccccagccaa aatggtcaaa tggtcagact ttgagcgtgc caccatccag gacatcttca 120
ccaagattga taaagatgtc gtagggccgg cggctctttc aagatgtctg atcgtctacc 180
cctggac 187
<210> 91
<211> 179
<212> DNA
<213> Oryzias javanicus
<400> 91
aagcagtggt atcaacgcag agtggccatt acggccgggg gatcctggtg ctgctgctgc 60
tgcttgcaaa cctcactttg gagataacct tgcaaaacct tcaacatggt tgacgccttc 120
tgtgccacct ggaagctggt tgacagtgaa aactttgatg agtacatgaa atcgctggg 179
<210> 92
<211> 151
<212> DNA
<213> Oryzias javanicus
<400> 92
ggaacgccgc ccgggatgct ttctgcctcc gggccaagat caatagcctg gcttccaaag 60
gagagtacaa gccctcaggt gcagccgacc agtcgtccat gcagtctctc cacacttcca 120
actacgtcta ttaaactcat tgaacactta t 151
<210> 93
<211> 146
<212> DNA
<213> Oryzias javanicus
<400> 93
attgtatcta tcaactgtgc taagtcatat ttaggggcgt tctggtacaa aaagtgtcac 60
cgcacgaacc ctaacggcgt ttatcgttgg gggagtgatg ctgcctttcg tgggattgga 120
gtggagtggt acgcctggaa gggtaa 146
<210> 94
<211> 143
<212> DNA
<213> Oryzias javanicus
<400> 94
cgtgggcttc gccaccaggc aggtcggtaa tgtgaccaaa ccgacggtga tcatcagcca 60
cgaaggagac aaagtggtga tccgcaccca gagcaccttc aagaacaccg agatctcctt 120
taaactcgga gaggagttcg acg 143
<210> 95
<211> 1542
<212> DNA
<213> Oryzias javanicus
<400> 95
gctgttgcca tgactactac acttactgcc agacagccga tgtccccaca acagccgagc 60
cctcatgtcg ctacaactgt ggtagctatc agggaagctg ttcctgctcc agctcctgcc 120
aatactatgg caactgttgt catgactact acacctactg tggagccagt acaccttcag 180
aatcatgtgg aggctcgctg ttcggctctg gcaccttttc cagcccgaat tatccctcct 240
actacaacga caacagccac tgtgtctggc agctcagagc ttcacacgac caaagaatct 300
acctggtctt cccattcctg cagctggata cctgctgctc ttgtgactac atctcagtct 360
atgatggacc gtcgatcagt tcgccacgtt taggaaaagt ctgcaacaat ggctcgagcg 420
ccttcttctc cacgtccgag tacatgaccg tcgtcttcca gactgacagt tctgtggttg 480
cacgaggatt cagtgcagag ttcttgagct ctttgaaacc aaattcaggt cgaacggatt 540
gctcctcaga cagcatgaac atcatcctgg accgtgctta cctgagctcg ttgggttacg 600
acggccacag tttgtatctg agggatcctt actgccggcc gcagatttcc tcgtacaacg 660
tggtcttcag ttcccgctca acacctgcgg caacgtcaga gaagttataa atgaccgaat 720
cgtgtacacc aacaacatct acgcttactc ctccacctac ggagagatca ctcggcaatc 780
tcaattcaag atgaacgtca cctgcatcat ggagaaggac tccatttccc agatcatgta 840
tgttgccgat ggcgagttaa acaccaccgt cactggctcg ggcagataca acaccagcat 900
ggatttctac acgtccggca gcttctatga caaggtgacc acatttccat atgtggtagc 960
gctcaacgaa tatctgttcg tccaagtgaa cttgaggaga ggtgacagca ctctggtact 1020
cttcatcgac acctgtgtga cttctccatc accttttgac ttccattcta gaccttatta 1080
cttggtcaaa gacggatgtc ctgttgacag cacctactac tctatcagta gtggttctcg 1140
accttacgcc cgtttcagat ttaaggcttt ccagtttctc cgagctacgg atcatgttta 1200
catgcaatgc acggtcctaa tttgtcccgc ctccgactac aattcccgct gccgccgtgg 1260
ctgcaggagg cggagcaaaa gaagcctggg gtcaaaccac gacacccaga ctctggttgt 1320
gggtcccatc aaacttaaag agcttaagaa agatgaggaa ggggccgaaa agcagaatga 1380
ggcgtaactc ctctgcagcc tacttcaatc aactttctat tacactgaat tatactcacc 1440
atctccattc ttcttcttct ttcttttaac ttgattaaag gcatcgacaa aaaaaaaaaa 1500
aaaaaaggcc gcctcggcca ctctgcgttg ataccactgc tt 1542
<210> 96
<211> 1504
<212> DNA
<213> Oryzias javanicus
<400> 96
aagcagtggt atcaacgcag agtggccatt acggccgggg actcagatct gcttttcatc 60
aagaaagatc tgcagcagac agagaagagg ttggaagctg agacagaaac atggcaagag 120
tgctcgcagc ggtgtgtgtt ctgctcactc tgcacatcgc ttcatctagc aagctggtgt 180
gtcacatgac caactggtcc cagtacagac ctggcagtgc tgcattcacc ccagagaaca 240
tcgatccatt cttgtgcacc catgtcatct acgctctggc gaccatcaac gacttcaacc 300
agatcgtcac agtagaatgg aacgaccagc agatgtattc gagccttaat agcctcaaga 360
acgcaaatcc tgcactaaaa actctgctgt ctgttggagg ctcagtcaac ggaatcagtc 420
cattcatccc catggtcgcc aaacctgaga gccgtgccgt cttcatcaga tctgccatca 480
gcttcctgcg caacaacaac tttgatgggc taaatctggc ctgggaatat cctggacaca 540
acggcagccc gatgcaggat agagagaggt tcacccttct ggtctcggag ttgtcccagg 600
ccttcataga tgaagccact gaaaacaaga agacgcagct ccttctttca gccaatgttg 660
ctgcactacc ctcatctgtc gacaaagcat acgaggtcaa caagatttcc cttcatttgg 720
acttcttgaa tgtcttgacc tatgacttcc atggatcttg ggagccagtt acaggacaca 780
acagcccatt gtatcgcagt gctgtggaca ctgcctcaca aatgcattat aacattaatt 840
cttcaatatc ctactggctg tctctgggag cagcagctga taaactgctg ttgggtttcc 900
ccacctatgg acggaccttt caactcagtg gatcagcaaa tgggctgggg gccctggcca 960
atgggcctgc agatgctgga ccctacaccc gcactgaagg actctgggcc ttctatgaga 1020
tctgtgactt caccaatagt gcaactgttt cctggatcaa cgaacaggca gtaccttatg 1080
caacctacgg caatgcttgg gtgggctttg atgatcagcg cagcttttcc tccaaggttc 1140
agtggatgac tgccaacaac ctgggaggtg ctcatgtttg gactctggac ttggatgact 1200
ttagagggtc tttctgctca gatggagctt ttccgctcat caaccacctc aggatgtcca 1260
tgggttttgc tccaaagcca acaacaactc ctcgccccac caccaccaga gaccctattg 1320
ctgacttttg ctatggccgc cctgatggac tttatgagaa ccctactgat aagaccacct 1380
acttccagtg ctacagggga aacacctacc tgcaccactg ccagcccggc ctcatctact 1440
gggactcctg caagtgttgt aactggcctt gagttggaaa ggcgtcacct atgaaaagat 1500
gctg 1504
<210> 97
<211> 1493
<212> DNA
<213> Oryzias javanicus
<400> 97
ggttgttatg attgctgtat ccggtgcatt ggggcagtgc cctacccgtc gctggtggcc 60
accctgctgt gctatgctgg catggcattg ttttgtggct gtggacatga agcgctgacc 120
cagaccgaat accttgtcga gacttacttt gcccggaacg tccaggacta cgtggtcatg 180
gcgtctttca tcaagtactt ccagtatgtg atctacggtc tggcatcctt cttcttcctc 240
tatggtatct tgttgctggc tgagggtttc tatgccacaa gtgcagtcaa gcagaccttt 300
ggcgagttca ggagcaccca gtgtggccgc tgcctcagcc tgacgtttat cgtagtgaca 360
tacatcttgg ccttcatctg gcttgtagtg tttgctttca ccgccgtccc agtcttcttc 420
ctgttcaaca tggagcaaac ctgccacaac atcaacctcc tggctgagac gacagccagc 480
attaatcagc acagctggat ctgtatggac gcgaggcagt atggtctgct cccttggaat 540
gcggtgccag gcaagagctg tggaatgacc ctggcatcca tctgcaaaac cagcgaattc 600
cacctgacct atgacttgta cattgctgca tttgccggag ctggggtcac tctgttggca 660
ctgtttctgt atctaattgc caccacctac aactacgcag tcttgcgttt cctgggcagg 720
aaaggaattc gttaatctgc cccattctcc ttcgtttacg ggcagcaagg tggacatcca 780
gctcacgcaa cgctagatct gaactcttca tctcccctta gaaaaaaagc acctttcaaa 840
cctggatcat ctgctgttct tgtagatgtt taataacaga caaatggttc tcatccctgt 900
catgaatgaa caacaggcag agatgaagat aattctgacc cagtaatgat ccccccggat 960
gaatctcttg cattgttcgt tttcctttta tgacttttca ttttatcctt tttgtaagcc 1020
tttcctattt ctggctgcaa tgtacgatac tccatgaata attaaaagct ttaccagctt 1080
gctttgcaag tcataactgt atgcacacta ttcagtctct gatgtgcata ttggttctga 1140
gcatgatttt gcggcttcct cattatcagc atcttacatg tcaacattgt ggatctaaag 1200
cgggggaaat tgaatgtgcc agattttctc tttgagtttt tcatccaaca tgaagttcac 1260
tgtgcattta tcactggctc ttattgattt gtttgaatgc cacagtctat ggatgggttt 1320
tagaaactag ctaaaaaaaa gtaataaagg gactctttgt ttctttaact agtctccagc 1380
tcacttgttt gtgtgcacat ttgtatttaa ttcaaataaa actttttaaa tcagaaaaaa 1440
aaaaaaaaaa aaaaaaaggc cgcctcggcc actctgcgtt gataccactg ctt 1493
<210> 98
<211> 1454
<212> DNA
<213> Oryzias javanicus
<400> 98
gggatcctct agggcagaca ttcgctgtga ttccttatca accaccaaag gctctgttgc 60
tgacagtcct ttcccatatt ctgaccatga agctctcact gctgagctga agctggactc 120
ttgtgtctca gccgagactg gaaatgatga agcattcaag acgccggagt cctctccagg 180
gaacctggct gagttggttg acattgtgac cgaggcccgc actgaagtca aagtgggctt 240
gcactgtgcc gagcggatgc gctacactgc agctcgtaca ggggtgatgg ggctggccct 300
gctgttcttg gagctgctca tcgctgcggt tccctggttc gctctgggag ccgaacagcc 360
cttcccacaa acctccttct acctgctggc agcgctgtgt ttcgccattc tactgaccac 420
atcgctgctc tatatctttt acacaatcga gatcaaatct ctccagggag ctgaagacca 480
gatgaggctg gcggtgggta gtctgcatga aaagctcagg ggttttcctg tgactcagcc 540
tcagagctcc tcccagaggt catcagaggg tcaggagccc cctgccttag acccagagga 600
ataatttcaa aataaaatta cttttttccc tactactaaa tttaatgctg agaaaaaaaa 660
gtgagacatt tctgaaagta gtacatgacg catgcgtcaa catttttata cttttaatca 720
tttgtaggat acaggtattc tccttagcac ctgctgctcc cacatatgcc tccttgcaca 780
tattttacac ctgcatggtg atgctgagct ttaaggcatt catgtgaatg tttgtgtttt 840
taaacttcag gtcggagttg cattccacca atctgcacgg gaccatttgt taaaatgttt 900
tccatgtcat gctttctgtt gttacattta aaaaagcaca gttagtgatt aaatactaac 960
agaaaaatct ttacctgtat ctcactccga atgatgaaga gtttacattt caggttagtt 1020
acaaggcgag aagactgatc ttaaacccgg ccgggttgtt tgcagctaaa tccaagagga 1080
aagagcaaaa tctttgtatg ttctgtacgc tcgacctctt ctaccgaaga ttatgttgga 1140
gataagctac actcctctaa acatccatgt atgtcctgac tatgcaatag agaatgttca 1200
atgcagtgac ttgattgtaa aatgtgtttt aaagatcagt tctttaaaat cttaagtgta 1260
gatcaacccc catgaactcc tttattatca tacaaataaa atgctatgat ttcttatttt 1320
tatcatgctg tgtaattgtg ttatgaagtc ggtagttctt ggttaaaata gctttccaat 1380
taaatgaatt ccaaatagaa actgtgatgc aaattatatt taaaagcaat aaaaaccttt 1440
tccatgacta gaag 1454
<210> 99
<211> 2034
<212> DNA
<213> Oryzias javanicus
<400> 99
tattccaact tcacaacaaa tgtgttgcct cgaataaaag acttgggtta caactgtatt 60
caacttatgg ctattatgga gcatgcttat tacgcctgtt ttggatatca aatcacaagc 120
ttctttgctg cttccagtcg gtacgggact ccggatgagt tgaagcagct gatcgacgtg 180
gctcattcca tgggcattgt ggtgctgctg gacgttgttc acagccacgc ctgcaagaac 240
acagaggacg gcttgaacct cttcgatggc accgactctt gtttcttcca ctcaccaccc 300
agaggggagc actcactctg ggacagccgt ctattcaact actccagctg ggaagtgctg 360
cggttcctgc tttcaaactt gcgttggtgg atggaggagt accgctttga tggcttccga 420
tttgacggtg taacttccat gctgtaccat caccacggta tcggcaccgg cttctccggg 480
gactacaatg aatactttgg cctgcacgtg gatgaagact ctttggttta cctgatgctt 540
gccaatcaca ttcttcatac cctgtatccg gactgcatca ccatcgcaga ggatgtttca 600
gggatgccag cactctgcag aaaagtgcag gagggaggac tgggctttga ttaccgactg 660
gctatggcca ttcctgacaa gtggattcag atcctgaagg agtttaaaga tgaggactgg 720
aacatgggca atattgtctt cactctgacc aacaggcgat atggagagaa gtgcattgcc 780
tatgcagaaa gccacgatca ggctctggtt ggtgacaaaa ctctggcctt ctggttgatg 840
gacaaggaaa tgtacaccaa catgagcgcc ttgattccca tgtcgcccat catcgaccgc 900
gggattcagc tgcacaagat gatccgcctg cttactcatg gtctcggggg agaaggctac 960
ctcaacttta tcggaaatga gtttggccat ccagagtggc tggatttccc cagagaaggc 1020
aacaatcaga gttaccacta cgcccgacgg cagtttaacc tggtggatat ggatcacctc 1080
cgctaccgcc agctctacgc ctttgaccgg gacatgaacc gcgtcgaaga ccagtacgga 1140
tggctggcgg ctccacctgc ctttgttagc gccaagcatg aaggggacaa ggtcattgtg 1200
tttgacaggg caaacgtgct ctttatcttc aacttccacc ccaacagaag cttccaggac 1260
tacagggtcg gtgtagaagc tgcaggaaaa tacaaaatca agctggattc cgacgaggtt 1320
cggtatggag gacacggacg cctcgaccac aacacagact tcttcacaga ggcccagccc 1380
ttcaacggcc gctccaactc catgctggtc tacatcccct gcagaacagc tattgtcctg 1440
gccaacgaag agatagatta ctgttattag aagatgattg tttccacaga gaccaggatt 1500
gagaatggat tcccaactca aactgagcgt taacagtcac cttcatcttg aagtgtcaac 1560
cttgaacacc agccaaacct gccttttgtg ttggagccct tttttttata ttttggtcta 1620
agtggaaggt taaagaggtg gtagaagaag aaaaagaaat tcaagattgt gggctttcaa 1680
gtaccagctg caaccttaga cactgtgtaa ctcgacttat acggcccatt tgggaagtgt 1740
ccaagcaaac agcatttacg tcctgaacaa caacaacctc tgagtccatc ctgaactgac 1800
ctgcaactgt ttgtgggttc agtttgtacc ttaaatacgt gtctggcaac tctggccagg 1860
aaaagcaata ccgtcacttc tcctttattt gaatgagttc aatgaaggtt ttgtgttgaa 1920
taaaaacaca ctaagcagta ttagctcatt gtgtcctgat acgttcacat gacaaacact 1980
gtgtatgaat aaattgtcat ttgcacaaaa gtaaaaaaaa aaaaaaaaaa aaaa 2034
<210> 100
<211> 1416
<212> DNA
<213> Oryzias javanicus
<400> 100
aagcagtggt atcaacgcag agtggccatt acggccgggg acccaagcag ccgcaccgaa 60
ccagcacagc cgcagcacca cctgtgtgtc atccacctcc cggcgacatg aactcccact 120
gcaacggaaa agtcagcgac tccgtggagg acttccacct caacaacggc ttcagcgacc 180
tcatcataga caccaagaag tccgccgtcg tggcgcaccg caagcacgag acgtcacgca 240
aggagtccga ggatgagtcc cgcttgcctc tgctggaggc cgcttacacc agcatcctcc 300
ggcacctcgg ggaggacacg gaccgggagg ggctgctgcg cactccactg cgcgccgcca 360
aagccatcca gttcttcacc aagggctacc acgagaccat cgacgacatc ctaaacaacg 420
ccatatttga tgaagatcac gaggagatgg tgatagtgaa ggacatcgac gtgttttctc 480
tgtgtgaaca tcacctggtc cctttttttg gaaaggttca catcggatac atcccaaaca 540
aaaaagtggt gggactgagc aaactagcaa ggattgcaga gatcttcagt cggaggcttc 600
aagttcaaga gcgtctgacc aagcagattg ccatggcgat atgcgaggct ctgcagccga 660
agggcgtagc tgtggtaata gaagcggcgc acatgtgcat ggtgatgcgc ggcgtccaga 720
agatgaacag ccgcacggtg accagcacca tgctgggaat ctacctggag gaccccaaaa 780
cccgggagga gttcctgact ctcacaatgc gcaactaaca agcctcaagc actattcctg 840
tctgcagaat tgttcagaaa gatattaagt gtgacttttt aaaagaaaaa aaccacaaca 900
ctgtgttagg aaatttgcag caagacttcc aactctgaag atccaaaaga gtgtgaagga 960
tttcagtcct gcagcagtgg cgatctcagt gaacacaaag atttatggtg cctttttttg 1020
tagatatatt agtctcacat aacattatta ttattataaa atatataaaa agctgagtgc 1080
aatattataa tgtgaaaatc ctttcaggtg gaaatttcat tggtgcttct ccaaactgtt 1140
ccactctttg ttatcgtgtt gagaatccac tgatttcaag gtttcaccaa catttgtgtc 1200
atttatatac atttttaaag aaaaatcttt gtattatatg acattttatg gtacaactga 1260
atttatatat ctgtagtcca acaaataaag ctaatgaatg tagatttcag atcgatgatg 1320
gtgatattcc tgaagagtcc taaataaaaa cgacaaacgg aaaaaaaaaa aaaaaaaaaa 1380
aaaggccgcc tcgactctgc gttgatacca ctgctt 1416
<210> 101
<211> 1375
<212> DNA
<213> Oryzias javanicus
<400> 101
aagcagtggt atcaacgcag agtggccatt acggccgggg gctggagagc tgtcgggacg 60
tcgctgcgga atctggggct gcacggtcgg gaagataggg atttttgtat catcccctga 120
aaacggtagt aatagaacac aacagggagc agcgatgcct ttcggtaaca cgcacaacca 180
gatgaaaatg aactactccc cggatcagga gtatccggac ctcagtaaac acaacaatca 240
catggccaag atcctgactc ccgccatgta cgagcgcctg aggagcaagc agacccccag 300
cggcttcacc ctggatgacg tcatccagac cggggtggat aacccaggcc atcccttcat 360
catgacggtg ggctgtgtcg ctggagacga ggagacgtac gacgtcttca aggagctcct 420
ggaccccgtg atcgaggaca gacacggagg atacaaaccc tcagacaagc acaagacgga 480
cctgaaccca gacaacctga agggagggga cgaccttgac cccaattatg tgctgagctc 540
cagagtgcgg acgggccgca gcatccgggg cttctgcctg cccccccact gcagccgagg 600
agagaggcgc atggtggaga agctctccaa tgaagctttg gactctctga ccggagattt 660
gaaggggaaa tattacgccc tgaagaacat gacggacgcc gaacagcagc agctcatcga 720
cgaccacttc ctgtttgaca agccggtgtc ccccctgctg ctggcctcag gaatggcccg 780
ggactggccc gacgccagag gcatctggca taatgacaac aagaccttcc tggtgtgggt 840
gaacgaggag gaccacctgc gtgtgatctc catgcagaaa ggaggcaaca tgaaggaagt 900
gttcaaccgc ttttgcactg gactcacaaa gatcgagagc ttgttcaagg agaagggcca 960
tgaattcatg tggaacgagc acctgggtta cgtcctcacc tgcccgtcta acctgggcac 1020
tggcctgcgt gccggcgtgc acatgaagct gcccaacatg agcaagcacg ccaagtttga 1080
ggaggtcctc aagagactgc ggctccagaa acgtggaaca ggtggcgtgg acaccgctgc 1140
tgtcgggggg gtgtttgaca tctccaacgc cgaccgactg ggcttctcag aggtggagct 1200
ggtgcagatg gtggttgatg gagttaagct gctgactgag atggagaaga ggctggagaa 1260
gggccaatcc atagatgacc tgatgcctgc ccagaagtga aatgcctccc cgccccctcc 1320
cccgctcacc ccctcgtctc cctgtggaac gactctcctg gaacgcactc tttca 1375
<210> 102
<211> 1371
<212> DNA
<213> Oryzias javanicus
<400> 102
aagcagtggt atcaacgcag agtggccatt acggccgggg gacagtgaag attatcctct 60
cttctctgcc accctcacca tgttggtcct gcagatgttg actgttctct ccttcactgt 120
tgtcttcctg gcatctgtgg aagctaaagg cgtgcagatg cagagggacg tccagtgctg 180
catgctgtac tcccagggca aggtgcgtac caaagatgtg ctgcggtttg aggtgcagac 240
ggaggggccc gactgcagta tacaagccat cattttttac acaaagaagg cggtgaaatg 300
cgccgacccc agagatcgca aggtgaaaag gttgttgagg aaactcctgc agaggcaaag 360
aaccaaagct caccgaaacc cgtggttctt ccgccatgag aacctgcccg tcatgtcaga 420
ggacaagaag gataactggg ccatcctgaa tgtggagtga tgaagcaaca tgaggtccgc 480
aggaaaactc gtagtcatac gccaagtaaa tgttcagctg ctcgttgtct tgacttgaca 540
acacttgctg actctccagt cgtcttgtgt ctctctttct accccgtctg tccctctgga 600
acaagacctg ggcagcaata catacatcac tggctttata tattccacca cgaactgctc 660
ctgaaacact cgccggggtg tttttttgtc ggactgatcc ttctaaaaaa tggcttatac 720
tgttatttgt cgaagtctgc ctgaaagcgt cgccctccac gacggtgtaa ccatggcgac 780
tgatgtgaaa tcagtgaaat ggtgtcctga aaaacaaaaa agcgttgaca ttcatatagt 840
atatcagcga gaatctctgt gcttacttgt gacttgtata acttaattat gtaataagac 900
atcacagtac agtaaagtag atttgtcaca tcgagtttgc agaacatctg ggagaacatc 960
acacctataa agctgttttc ccttttagat agtggtgata ttagaagtct cgttcgggct 1020
atatctaact gtatgtggat taaaaaaggg accagagtga aggtttctat gtgggttcac 1080
tctagttttt aacacagcaa agaaaggttc ctatatcttt tgtgcgttgt ccatacaaca 1140
acttgacttt ttaaattcga cctttcaacg tgaatgcttg acgtaaaaat ccatttgtgg 1200
tttggattta aaatgcttgt ccgtcaccaa actatgccaa agtattcatg agtccattct 1260
tcaaatgaca aggctgttat tcttcgaggg ctctggtgtg tttcacacac acacattgta 1320
ttgtagaaga atgcaatgta tttcaagtaa aatattttat aaatttatct g 1371
<210> 103
<211> 1272
<212> DNA
<213> Oryzias javanicus
<400> 103
aagcagtggt atcaacgcag agtggccatt acggccgggg agagaaatag tattggagag 60
gcagggcaga ggttcagata aaaagagcca cagaacttat tggctttacc cctgacaaac 120
aactaccaga cctccagagt aaaaatggat ggactaaaac agtggatcca agtggctgtc 180
gtggccctcc tggtgttaac agtgatggct gcagatggag gcaaagctga gaaacaagga 240
aagaaggagc gcaagtcaga ctgtggggat tggcagtgga gcgtttgcgt ggccaacgag 300
ggcgactgcg gacttggcac cagagagggg acacgcaccg ggaccgactg caagcagacc 360
atcaagaccc aacgctgcaa gatcccttgc aactggaaga aaaagtttgg gggggaatgc 420
aagtacgact tccaggcttg gggggagtgc gatctggcga cggggatgaa aaacaggacg 480
ggagttctga agcgggcgct catggacgcc acctgcaccc ccaccgtcac ggccaccaag 540
ccttgtggga aaatccccaa gaccaagctg caagatgcaa aaaagcagaa gaaggaggga 600
aagaagcgag agcgcgcccc catgccctgg tgaggttgaa ccatgacagc ggcgggggac 660
cacagagaag aagtccaccg ccatacttta acacggttcc tcattatgtg tctagtagct 720
tgactatagg agcctatata cttttgggga agaaaaaaac aagtgtggaa gtacgtcttt 780
tagtgaaaaa cgtttcatct ttccttcttt ctagcacctg atacagaact ttttgtagga 840
gacgatatag gccgcggttt ctctagggct taattatgtt attttttaga acaaattgcc 900
tcgtctttgt tctacagttg cacaccctaa ctctaattcc ggaaaacgtt ttgctctgcg 960
gtccgaccta atgaagcatt ttgttaaaag tgaagtggaa aacaccctca cgtaattaat 1020
ttggaagtca aacgtttgcg ttcctggagt tcactgtgac tgtgttctgt tgttaatgga 1080
agttgcatga aataaccgca gttgttttta gttgtttctg ctgctctgtt ggatctcagt 1140
tttatccgct ctctgtctat gtatcatcat ccacaaccta tgctttctgg tatatttgca 1200
aaaacaaaaa taaaagaaaa aaaaaaaaaa aaaaaaggcc gcctcggcca ctctgcgttg 1260
ataccactgc tt 1272
<210> 104
<211> 1262
<212> DNA
<213> Oryzias javanicus
<400> 104
gcagtggtat caacgcagag tggccgaggc ggcctttttt ttctttgaga tgcaaaactt 60
ttatttgaat caaacaatat caattttact tttgtttatg tgaacttttg aagaattaaa 120
accacaaaat gtaacaaact acaatagatg attttgcggt ttcagtgttt gtgcatcgtc 180
ttcttcgctg ctcgtctccc ctctagttct ggccttggag ggcttctgcc atgaaaagct 240
tcccgaggtt ctgcgaggtg aattccttca cattctggca gtagttatta atttgccctg 300
caataagcag cgtgtccatg cgtggaggcg gctgaggggg cttgaacaat ttgttgatgt 360
cctcctcagg cagcgcggcc tctccccggc tctgtcgctg ggcgttctcc tgctgccgcc 420
tctggagata ctgatgcttc tgctgctgct gcttactcag gttgcgactg taggtgttgt 480
atttgacaat ttcctggctc atgtcatcca ctctgtccat gagcagctga aggctcttct 540
ccaagtgatt gctgctggag aggttgagca gttcgtgctt gtcggcaacg gtggacttat 600
cctccagctc ccacatcagc acgctgatga ggtgggagtt tttgatgacg atgggcacct 660
cctcaaacat gttctcaaat ccaatgctgg ccttcttcaa gccctctggt gtgaaatctt 720
tctctttgca gatctccatg agtttggggg tgagccggta ggctttgaga gacagggagc 780
cttgggctgt tttgatggga tcgtagatga gaacgaccga ttcctcgatg gcatgctggt 840
agctgaactg agagtccagt agagctctgc tgacaaacga gccatagagg gtggactggt 900
accagcctac atgcagatgg tcaatgttga cgtgacgcag ggaacgcatc atctccatct 960
ggtactggac ttcgtcgaag tctgcatcgt cttcagtgtg ctgggggaag gggaagcagt 1020
tggtgatctc cagccggtcg tctaccacca gacccagcag gacgccctga accacttcgc 1080
tgccctggcc ctcttcctgg tagtgcttaa ttattttcag caccaccaat ccctcgatct 1140
ggacctgctt gactggagag tccagcgtgc cgccagtaga agccattcca gaacctgatg 1200
ccgagctgag ttctttacgc gaagccatga gtccctcaca ccaaaaaagg aaacattaag 1260
gg 1262
<210> 105
<211> 1227
<212> DNA
<213> Oryzias javanicus
<400> 105
agtgtgttcc tgtgacagca aagacaccat cctctcttgt aacgtggagc cctacgtcct 60
gcctgctggg cttagcactg gtgccctcat agctattctg gcctgcattg tcatcttact 120
cgcaatagtg gtgctgtttg tcgctctgag gcgtcagaaa aaggagccgc tgatcgtgtt 180
cgaggaggag gacatcaggg agaacattat cacttatgat gatgagggag gcggtgaaga 240
ggacactgag gcgttcgaca tcgctacact tcagaaccca gatggtgcca acggtttcct 300
gccgaggaag gacatcaaac cagaactcca gttccccctc aggccgggca tgggtcgacc 360
tgcagccaac agcgtcgacg tggacgactt catcaagact cgcattgcag acgccgacag 420
cgacccgacc gcacctcctt atgactccat tcagatctac ggctacgaag gccgaggatc 480
tttggctggg tcgctgagct cgctcgagtc tgtcacaaca gagtctgact tggattatga 540
ctatctgcag agttggggcc gaggtttaag aagctggcgg atttgtacgg gaccaaagac 600
tgctccttta acgatgtgga cgatgcggag gactcttaac agaggtcttc ttcagtggac 660
gccctccaga tttgtgattt attgattcca agaggatttc ttaactgagg gttaagactc 720
ctcagtatcc tgttgttacc atcttccagc tgattagttc gatctgtcaa tgaatggaca 780
cgtattaaat gtctggactc ccattattcc aaaatctgtc ccgtccttcc tatggctttc 840
tttctgtctt tttctacaga cctgagcatt gcaggactgg cagatttgtt accgcatcag 900
tttgtctgtt gggagcaata tgatggaaat gtggttgtat cctgagaatg acttgcctac 960
agcagatttt ttgagtgtgt acgacgttct atatctataa aaggactgga ctcatcctca 1020
gaggttcctc tgttccccca gaggaagaga agaaacatca aaatcaaaga aaagttgtat 1080
aaaggtgtta gtagggtcaa atttgttgtt ttgtctgctg aagtgcagta gatgcgaaga 1140
ccgactccgg gttgttgata ctctgaagac gctcacagca ttttcacagt atggtggcct 1200
tataataatt caacaacaac tccagga 1227
<210> 106
<211> 1212
<212> DNA
<213> Oryzias javanicus
<400> 106
agtttgtgtt tgacattgat aagacagatc acatggacgt cttgcctatc tgtcatcgct 60
caggctttca tcgacgcttg ctccatttct gacctgcaac taggcaagga ttctcctacc 120
atcaagctac tgtatgccaa ggaaatccct gagtacaaga agagagtgca gtgctactat 180
caacagatcc aggagatgcc tcctctcagt gaacaggaga tgaacgctca cctggcgaag 240
agtcaagaaa gtaccgaaac gagtttaaca ccaacctggc cttgacagaa atctacaatt 300
acgccaagag atacagaaac caggtggcga acgctctgga ggctaacccc accgcacgtc 360
gcactcagct gcaccacaag ttcgagcagg tcatcgctct catggaggac aacatctatg 420
agtgctgcag cgaagcttga cccctgtcaa agccttcttc ctttttctcg ttctgccagc 480
tgccttcctc ccacacgcgt agtttcttgg gaatgaaagg agatattgtt cgcgaccgcc 540
tttcccacag actgttaata aggtgaccag tggaggagtg acggactaca acctgcctct 600
cttgtgtcgc gtgacacaga tagaattcaa ctccggctga gccattcttt agaaggaatg 660
tggaggagct cagagctttt tctcggatgt cttcacaata aacgggacct atacagtaag 720
ccctggtggc aatcttaacg cactgagccc ttttctggag ggaatgagcc aatgatgcac 780
tgtactgtaa tataatgtca atagcaatat ttatagatgt gttataattc tctcaagtgg 840
cctgttgttc aggatgagta ggacctggca gactccttcc atctcccaaa acctgcaatg 900
ttctcaggtg tggtcgatga agacgagcga ctgactccct ctggtgcttc gaacgttacg 960
tcccagtgtg ctttgcaggt tgatgaaggg ttgaggagga gcagggagaa taaaaaacaa 1020
ggactgggaa ttctgatatg agatgttttt tgatgtagtt cactgggaag agctgcatca 1080
gtgtagaagt gttaatcagt gtgcttgtga acaaatgaat ttaaatgttg gaatcgattt 1140
aaagagggaa aatttacatt aaagaaaata agtgagctca atataacctg aggagaaact 1200
ataaattgtg at 1212
<210> 107
<211> 1207
<212> DNA
<213> Oryzias javanicus
<400> 107
aagcagtggt atcaacgcag agtggccatt acggccgggg ctttatcgtg tggacggacg 60
aagaggggga gttgttatta gttggtactt gaacttgcct tttattcgga accatgccgg 120
gtccggacaa agaaacagac ctgacggaga ggctggaggc tttcctgtct gatctgaagc 180
gcgggggcag cgggaccgga cccctgcgga gctctgcgga gacggcccgg gaaacgacag 240
ccctgctccg caggatcacc gcacaggccc ggtggagcag cgcaggagat ttgatggaaa 300
taatccgaaa agagggaagg agaatgacgg ctgctcagcc ctcggagacc acggtgggga 360
acatgatccg aagggtcctg aagatcgtca gagaggaata cgccaggtca cgagggagca 420
gcgaggaggc ggaccagcag gagtctctgc acaagatgct gaccactgga ggactcagcg 480
aggagaactt ccggcagcat tttgctcctc tccgagccaa cgtcatcgag gccatcaacg 540
agttgctgac tgagctggag ggaacgacag acaacatctc catgcaggct ctggagcaca 600
tccactccaa cgaggtgatc atgacgatcg ggcgctcgcg caccgtggag gccttcctca 660
aaaacgccgg ccgcaaacgc aagttccacg tcatcgtggc ggagtgcgcc cccttctgtc 720
agggacacga gatggcgact aacctgtcga cagacggcat cgagacgact gtgatcacgg 780
acgccgccat ctttgcggtc atgtcccggg ttaataaggt catcatcggt acgcagacgg 840
ttctggctaa cggcggtttg agggccgtga acgggacgca cacgctcgcc ctcgccgcca 900
aacaccactc cacgccgctc atcgtttgtg ctccgatgtt caagctctct ccgcagtttc 960
caaacgaaga ggacaccttc cataaattcg tctcccccca cgaggttctc ccgttcactg 1020
aaggtgagat cctctcaaag gtgaacgtcc actgtcccgt gtttgactac gtccctcctg 1080
agctcatcac cctgttcatc tccaacatcg gcggacacgc cccgtcgtat atctaccgtc 1140
tgatgagcga actctaccac ccggaggatc acgagctgta atccacacaa caaataaatt 1200
attcaca 1207
<210> 108
<211> 2040
<212> DNA
<213> Oryzias javanicus
<400> 108
cgaggagttt gtttcgtgac tttgaagttg tgacgagatt ggggggtaaa taatgccaca 60
ctccttgtgg ctcgatccgc tgtcacccgc acaactcaag cgcctggagg agcataaata 120
cagcgcctcc ggtcgctcgc tgttcgagcc gccgtgccag attttctgga actggctcgt 180
ccaacaaata ccgacatgga tcgccccgaa cacactgacg attgtcgggc tggtggtcaa 240
tgtagtttca acagtcatat tagtatgcta ttgtcccacg gcgacagaag aggctccagc 300
atgggccttc atcctgtctg ctctgggtct cttcatttac caatctctgg atgctattga 360
tgggaagcag gccaggagaa caaacagcag ctctgcgctc ggcgagctct ttgaccacgg 420
ctgcgatgcc gtttccacag tgtttgttgc cgtgggaacg tgtatatcct gtggaatcgg 480
aagcctccca aactggatgt tcttctgcgg tttcatcggg atgtttatgt tcttctgtgc 540
ccactggcaa acctacgtct ctggaaccct tcgatttggg ctggttgacg ttacagaagt 600
gcagtttgcc attatgatca tgtacctcat gtcggctttt ggaggcgtga gcctttggca 660
ggccacgctg cctgttttag gactgaagct atacgtcttc cccatcatgg gaatcaccgg 720
cggtgccctg tactcctgct acaactattt ttatgtcatt ttgaacggag gtgttgggaa 780
gaatggctcc actgttgccg acaccagcgt gctgactcca ggtttgcaca tcggcctcat 840
cctcacgctg gccttcatca ttttcaagaa gtcctccagt catctgttcg aacaccaccc 900
ctgtctgtac ctccttgctt tcggcatggt catctcgaag atctccaata agcttgtgat 960
tgcccacatg accaagagcg agctgcacct cccagatacg gccttcatcg ggcccggcct 1020
cctcttcctc aaccagtact tcaacagctt catcgacgaa tatatagtac tctggatcgc 1080
catggtgctc tctctggtgg atctaactcg ctactgcaca ggcctgtgcc tccagatcgc 1140
cgcccacctg cgcatccgag tcttcagcat cacgccgccg agcagcacgc accgcgactg 1200
acgctttgcc cggcgggagg aggcggcagg agacgaagat ctctccccgc tcctaaaagc 1260
agacgatgca gagaaaccct caaccccgag cctcgacgac gtcaccactt taaattaaaa 1320
ccgcaaagaa atccactaaa ccaaaaagaa atacaaccaa agttgtctca caaatctaat 1380
ttaaagaaat ttttactttt aaatttgtgt tttggtcact ccctgctttt aacttcaaag 1440
ccaaaatatt tttcttaaat atgatttttc ttttatgcaa tctgatcata atcagggatt 1500
taaaaaaaaa aaaattcgca gtttaattca aataactacc aatgaatcgt gtggtgtcct 1560
ccaggggtca tggggtcacg tgttcatgcc gaaaacgacg acgggtcctc tcttaaggcc 1620
actcccacta acaccactcc agttgtcaga gttgaaccct tcattcctcc catttgacca 1680
tcctgtaaac gtagaactct caccttagcg tggcctttgg agcgccgtcg gctgatgaag 1740
catcaacacc gcctcgctct gctttctctc ttcttctccc taaagggcta attgagcgtg 1800
attgtacata aacgaactaa agacgcctta aatatttttg ttgtaattac tgttgaactt 1860
gtgaatattt aaagcaggac tgtgtgtttt tgtatgtttg ttataccaga cacagcaata 1920
gtttaaacta atttttcttt tgttctttct tgcctgcctt gcatcttttt ttcattcttt 1980
gggttattta atagaattct ttgggagcta aagggacttt tctgttgggt tggcaaacgt 2040
2040
<210> 109
<211> 1103
<212> DNA
<213> Oryzias javanicus
<400> 109
aagcagtggt atcaacgcag agtggccatt acggccgggg acaggcggac cgggaggcgc 60
acgcggcggc tgctcttctg cctgaccggg tgacggagat cgggggcgcg cggaccgtgt 120
ggacaccccc ctccctcacc ctcaaccccc ccgggcctcc acgagtttgg cgccacggac 180
gcgggaaaac ggtgcagagg acggggcgga accagaactc tgactgacga gggcggagct 240
acagagaagg cggggctcag gagacgcggg tcagagcggc catggcgagc aacaacacgg 300
cgagcatcgc gcaggccagg aagctggtgg agcagctgaa gatggaggcc aacatcgaca 360
ggatcaaggt gtccaaagcg gcagcagacc tgatgtccta ctgcgaagct cacgccaaag 420
aggacccgct gctgtcgccg gtgccggcgt cggagaaccc gttcagggag aagaagttct 480
tctgctccat cctgtagcgc cggcgccgcc cgccgtccgt ccgtccgcga ggagctttga 540
acgccaaagt tcaaagggcg cagaaacctt ctggtggggg ggcatgaagg tcgccggcct 600
cctcttttgg acccgaacgt tccgagagca aaccgcagat ttagcatgta tgtattaatc 660
agaccgattc ggggggcgag ggcgctctga tgacatcacc gggagaaacg gcgggaaacg 720
gagcgccagc cgtcttcagg ttctagaaac tttagaggga aatttaaaaa gtggttcttt 780
tgggccttcc aggtgatctg tttcaagggt gggtggagct ccggaggacg acacaccaag 840
ccccgcccac cacctgattg atggaatgtt gttcagtttg tgttttctcc acaaatatcc 900
gtgtagccgt acgtgagtga agtctgtcga ccttttttta cacccgcagt agtgagagct 960
tttctatgga gatgtttgga ttctgtgctt ctctgtgtgt ccacacctgg accgcgtgat 1020
ccgtggcgtc cgtccgcttc agactcagac cttctttcta ctctttgtac taaacactag 1080
acagtaaagc gtgccgtgct gtg 1103
<210> 110
<211> 1083
<212> DNA
<213> Oryzias javanicus
<400> 110
aagcagtggt atcaacgcag agtggccgag gcggcctttt tttttttttt ttttctcata 60
ttattttatt tgtagcttat agaccatact aaaacaatat atttaaaata cacattagta 120
ctttttccat aataattaat acattctatg acagtgataa gggaatgaat gtgtatgtgc 180
catcttaagc caggagcagt gagtagtgtt aggcgcgggc agagatcggg tggcttaagc 240
cgctgaagta agacttctcc cgctcgctca tcagctcaaa gtgcagcggc tgctggcaga 300
aggtgcactc ggccgacgag tgaggcttca aatccagacc cacctccttc cacgtctcaa 360
caagtctctc aacaaagtat ttcatcatct ctggggtgtg gtgaggagtt ggagcaatgc 420
gcagcagctc ttctccccgg gcaacggtgg ggtaattaat ggcctgcacg taaatgttgt 480
gccgactcat catgatgtcg cacacctccg tgtttttctc agcattggag acccggacgg 540
ggatgatgtg gctggggcag tgcacgacgg gtagacccga gtccatcagc atctgtctca 600
gcagcttgac gttgcgctgg tgtttgcgcc tgagcgcgcg gccctcttcc cctttgagga 660
tttggatgga ctgcctggct ccggccagca gcatcggcgg caaagaggtg gtgaaaatga 720
aaccggcggc gtaagagcgc accgtgtcca ccagggcgga ggtactggca atgtagcctc 780
ccacacagcc gaaggccttg cctaaagtcc ctgaaacgat gtcaatcttg tgcatgatgc 840
cgtccctgtc gccgattccc cctcctctgg cgccgtacag gccgacggcg tggacttcat 900
ctacgaaggt gatggcgcca aactcatgag ccaaatcaca catctcttcc aggggacaca 960
cagctccatc catggaatgg accgcctcaa aagccacaat cttcggtttg ctggggtctc 1020
ccttttgcag caactctcgg agatcccggc cgtaatggtc actctgcgtt gataccactg 1080
ctt 1083
<210> 111
<211> 1060
<212> DNA
<213> Oryzias javanicus
<400> 111
gtcgatgtaa cggtccgcga ggatggacgc agcggagtgc ggggtccggg tgatgtgccg 60
ctttcggccg ctgaacgaca cggagcggag ccgcggagac aaattcatcc ccaagttcag 120
cggggaggac acggtggtgc tggcgggaaa gccttatatg ttcgacaaag tccttcctcc 180
aaactctgaa cagattcagg tgtatgactc ttgtgcaaaa cagattgtca gagatgtatt 240
gggaggatac aacggcacca tctttgctta tggacagacg tcctcaggaa agactcacac 300
gatggaggga aacctgcaca attcccggct gatggggatc atccctcgca tagccagaga 360
catctttgat cacatctatg ctatggatga taatctggaa ttccacatca aggtttccta 420
ctttgaaatc tatttggaca aaattagaga cttgttggat gtatcaaaga ccaacttggc 480
agttcatgag gacaaaaaca gggttccatt tgtcaaggga tgtacagagc gcttcgtgtc 540
cagtcctgag gaagtcatgg acatcattga tgaaggaaaa gcaaaccggc atgttgctgt 600
cacaaacatg aacgagcaca gttcccgcag ccacagcatc ttcctgatca acatcaagca 660
ggagaacgtg gagacggaaa ccaaactgtc tgggaagctc tacctggtgg acctggctgg 720
cagtgaaaag gtgagtaaaa caggtgcaga gggagccatc ctggatgaag caaagaacat 780
aaacaagtct ctgtcagctc tgggaaacgt catcgctgct ctgagtgaag gaacgaaagg 840
acatgttcca taccgagaca gtaagatgac tcgaatcctg caggattcac tgggaggaaa 900
ctgtcgaacc actatcatca tatgttgttc cccatccgtt tataatgagg ctgaaaccaa 960
gtccaccctc atgttcggac agagggccaa aactataaag aacacggtgt ctgtgaatct 1020
ggagctgact gcggaggaat ggaagaagaa gtacaaaaaa 1060
<210> 112
<211> 2040
<212> DNA
<213> Oryzias javanicus
<400> 112
ttagccagcc tgcccaaaca catcgaccat ttctccaagt tttcaccttc gccgctttcc 60
atgaagcaat tcatcgattt cgggtccatc aatgcctgcg agaagacatc ctttgtcttt 120
ttgaggcagg aactgcctgt gagactctca aacatcatga aggaaatcaa ccttctgcct 180
gacaaactgc tcaccacccc ttcagtgcaa atggtgcaga gatggtacat tcagagtctg 240
atggagatcc ttgagttcct ggacaagaat cctgatgacc aaaagatcct cggagagttt 300
gcagacgcgc tggtgacaat ccgaaacaga cacaacgacg tggtgccgac aatggctcag 360
ggggtcatcg aatacaaaga ggtctttccc caggatgtgg tcaccaatca aaacatccag 420
tacttcctgg accgcttcta catgagccgc atctcgatcc gaatgctcat caaccagcac 480
actcttgttt ttaacggcac cacaaacccc gcccacccca ccaccattgg gagcattgag 540
cctcactgcc aagtcggaga agtcgtgcaa gatgcctttc atagtgctaa gatgctgtgt 600
gaccagtatt acctccgctc ccctgatctt gtacttcaag aaatgagcca caagaagaag 660
ggtcatccca tatctattgt atacgttccc tctcacctgt atcacatgtt gtttgagctc 720
ttcaagaatg caatgagagc tacaattgag acccatgaga ataagaacaa cctaccaccc 780
attaaggtta ttgtgtctct gggcggtgaa gacatgtcca tcaaggttag tgacgtgggc 840
ggcggagtcc catttcgcaa gatcgagaac cttttcagct acatgtactc cacagctcct 900
gccccccaga ttggagagca ctcacgaacg ccactagctg gcttcggtta cggtcttccc 960
atttctcgcc tctatgcaaa atactttcaa ggggacctgc agctttattc catggagggc 1020
tacggcacgg acgctgtcat atccttgaag gcgctgtcca cagactccat tgaaaggctg 1080
ccagtgtaca acaaaaccgc cctgaagaac tataaagtgt gccaagaagc tgatgactgg 1140
tgcgtcccca gcaaagagcc cctagatctg cgtaaataca gggtggccta gtgaggtttc 1200
agcccaggtg gtgcttcaag acgacttaca gcggagcctc cagagcgtgt ctcctatatt 1260
cccatcacct catcatctgc cggggcagat gaacaccgga ccttcccaga cctggagaac 1320
cctttcaaga ccagcctgtt tttagcatca gcatgttgtt ttcagcttct gtcatcctgt 1380
tggggttttt gtcatagacg gctacaaact ggatgtttgg gagtgctgca tcaaactggg 1440
attgactgag gtcctgcaaa ttagagttgc ctgcggggcg gccaaggtgg cgcccggcct 1500
gtcgcaggtc agcgaaagtg gaacgaggag aagaggaagt catccattga acatgaggac 1560
tttgccaaac agggaaaacc tgttgatgat gccttatagt agcagattca cccaaaggtg 1620
tgtgtcacac ctgaaccacg agaacgaata cttgcacctt gttaaatttg agagtactgt 1680
aacgccgctg gctctgcagt tctgaacaaa tgtaagcctc tgcgtgcttt gtgcatgaat 1740
acttgtgcta gcttcttttc atttcagcct tcaaaacaag aatccttgac tcagaatcca 1800
tttttttgga ccaggctgaa tatagtccat tcatttgtaa caagtacgat tgcttcactg 1860
gggaatgtgc agctccagag ggcccacttt cagtgtcgca tcacaaccag ccgtgagagt 1920
atttagcagc ttcctataga ggcggctgtt ggtaaacctc caaagggtgc cacgtttgag 1980
tttttcttcc agtttgtgat tcatcaaggc cagtttctga gtatttttcc tattctgtgc 2040
2040
<210> 113
<211> 946
<212> DNA
<213> Oryzias javanicus
<400> 113
gctcgtgctc atcatgaggg ttcttgccgc gatcctcgct ctggctgtcc tctcagggtc 60
ccacgcccgc agcgtgcccc tggaggacct gcagaacacg tgggaggtga ccgtggagaa 120
gttccagaat tacatcacag acctgaacac aaaggctgac ggcgtgctgg agaacatcag 180
gagctctcag atcagcagag agttggaaac tctgatccag gacagcatgt cagagctggc 240
catgtacaga gacgacctgc agaacaagct gggcccctac acccagcaga gcgcggagcg 300
cctgagccag gacctgcagc tgatggcgga caaactgcgc gagaacatga atgaggcgcg 360
cgagcaggtg gaccgctacg tccaggagct gcagaccatg atggagcaga acgccgacga 420
cgtcaagctc agggtgtcca cctacagccg caagctgaag aagcgcctca acaaggacac 480
gcaggagatc aagaggtacg tcagcgagta cgtcgaggag ctccactctc gcacctccga 540
gaatgtggga gagctgaaag cccgcctgga tccgtacttc tctcaggtcc gggacaacgc 600
ccaggccaag atctccaccc tgaacgagct gctccagtcc cagatcgaga acgtgaagac 660
cagggtgcag accacccacg aggacatcaa ggagcgcgtt aaggtcgcca ccgaggacct 720
gcgctcttct gtggagggaa ggattcagga ggtgcgcaac tggttccagc cctacgtgtc 780
catggtcacg gagagcctgt agataagact ggtgacgacc cctcatggaa acatgttcag 840
actgaaacca acaagtctgt ggggaagcat gaaatattaa aagatactaa aaacaaaaaa 900
aaaaaaaaag gccgacctcg gccactctgc gttgatacca ctgctt 946
<210> 114
<211> 2040
<212> DNA
<213> Oryzias javanicus
<400> 114
gcacatcagt catggctact gacggaggaa gcgttgaaaa agacagcaaa agagaaacta 60
agcacacggg gactcaaatg actgagacgg attctttcaa cacattacct gatttgaatc 120
ctgctgaaga gtacaagatg aaccgcggac gacgaggcct ggccctcatt tttaaccatg 180
agcgatttta ctggcggttg gggttgaatg acaggagtgg aacaaatgct gaccgctaca 240
acctagagaa acgactccag gatttaagtt ttgatgtccg gagttatgac aatgcaaaac 300
agacagatgt cttggataga atccacgaag ctgctgagga ggatcactct gatgccgact 360
gctttctgct ggtcttcctg agtcacggcg agaacaacta cgtttacacc tacgatggca 420
tgatcaacat ccaggagatc actgctgctt tcagaggaga caagtgccgg agcctcatag 480
gaaagccaaa gatctttgtc tttcaggcgt gtcgtggaga caaacatgat agtccagtgg 540
ttccatccga tgctgttgac cataaagaag tgatcaatga ggtggtgatt gatgccagtg 600
cactaaacac tcttcctgcc ggagctgatt tcctcatgtg ctattctgtg gctgaaggtt 660
tctattccca tcgagagacc atcaatggct cctggtacat ccaggatctg tgcgagctcc 720
ttaaaaagta cggcgatacg ctcgagttca ccgagttgct cactctggtc aacagaaaag 780
tggcaatgag aagtgtgggg atgtgcagag acccgaatgc tattgggaaa aagcaggtcc 840
cctgttttgc ttctatgtta accaagaagc tctacttccg gaaaaagtaa cagcttcaag 900
tcgtcataaa atcaattgat tttagcttta aaaaaaaact taatttttat tccttacctt 960
taaagaccca ctccgatcat cttttgagtt attttaaaaa cattcctacg attcttttca 1020
tcatgattat gctgttttca gtcagatacg ctctttttca aattatactt tgttgtctgt 1080
tcctgattca caacaacttg aatcaggaaa aaaatgccac aataacatgt taaatacacc 1140
attcaattgg agtgggtttt ttatatcact gtgaacactt tgaaccagtc tgtgaaatat 1200
tgagacacca ccggtcatct agaggattac agaaacggct cccattttca gtcctgatgc 1260
aatggagaag tccagcaaac actcttcagg tcacatctct cttgtaagca atagttcaga 1320
caatactgga gtccaacaca acagaccctg ctggaattca ggttaaagat gctggcaatg 1380
cctgttttac taactccttc acaggctgtt ttttttttta cttttttata cacttgcatc 1440
cctgctgtag gatccctccc ccctaaatcc agttaaaata ttccagtttt atataggaat 1500
cttcttgaag ttcctcaact gtttgcttta gtttgtcact tttatcaaaa caataataac 1560
cttcatttag gatttttgtg aaaaccgtca tcatcattgc tgtccactgt ttaatgatgc 1620
tcaggtgttg gttgcttcca gtacttcaaa aaggactaag atttagctaa tgcatctacc 1680
tctgcagcat tcagttatga catatattgc cagttttgtt agtttaggtg agtgttgttt 1740
ttcaagcatc tttaaaagga caatcaggga aataatgtca caacaacacc acacacccat 1800
agagacaaac tcaatcattc caaaccactt ctttaaaagg gaattcatgt ttgtgctgtt 1860
ttgtcacagt agttaatggt actttaaata atgcattttt gcaccaagtt ggttaaaaca 1920
atcactaata tagaaaaatc tcagggagga ctgtgtttac tctctttttt ccacaatggt 1980
gtgacgtggg tctgaattgt gcaaactgtt gctctactgt tttgggttgt gtctttctgg 2040
2040
<210> 115
<211> 1014
<212> DNA
<213> Oryzias javanicus
<400> 115
atgtaggttc agtgtgcgcg gaagacgctc tcatctggtg cccatcttca acgtgtgcgc 60
ttttcccaac caacagctga tttatggaga ctctttatat agtaacgata tatcgagaca 120
cagtggcaca cttctcctgc aatcatacat cacaggttta tctcccccag agtgcacttt 180
ggctctgaga gctacaagtc aacacattta cactcctata taagcaggtc gggccagcgc 240
tgccttcaac tctgttttcc cttcacagac atttctatag cggcaactat aaataacagg 300
atgaataagc tgctggtcct ttctgtgctt gttgcacttc ttgctcttag tgctgaaagt 360
tttcgagtcc ccagacagac tgaggaggaa gagggttctt ttactaaaat gacaggcaag 420
atcaagtcct actacagcag tgctgtcgac accgtcaccg gatatgtgga cagcatcaaa 480
ggcctgaagc tggaggagaa agcaaagaat ctttactcgg acacaaccaa catcgtgagc 540
acgtatgctg gcatcgtcca agaccaactc taccactttt tccaccacta ggtacacaac 600
aaagctacaa atatacaact tcagttgacc ttggctgcgc tttctgacaa gataacagca 660
ctcactctgc agatgagaga gctttggaat ctttctttac tccgccaaaa aaaaatataa 720
taaaaaggtt tgagcgtcca cctctgaaag cctcaacatt tgtcaagaag aacaagatgg 780
gaactgatgg cagagaaatt cctttgtggg tctgggagac agaaaccaat gacactgaaa 840
aaagtattgc aaacaagctc tttgtccatc ccgcttccca accgttgact taagtaacca 900
ttctcccact gaatgagtat taatatttgt gtcatatctt taaataaaat atttatataa 960
aaaaaaaaaa aaaaaaaagg ccgcctcggc cactctgcgt tgataccact gctt 1014
<210> 116
<211> 999
<212> DNA
<213> Oryzias javanicus
<400> 116
catcactggt ggatctgtat ccgtccttcc taaggaaggg ttaccgcaga gaggtcttca 60
tcgctatcat ctgctgcttg agctacctgt ttggcctcac tatggttaca aagggtggca 120
tgtatgtttt ccagctgttt gactactatg cagctagcgg tgtgtgcctt ctgtgggtgg 180
cattctttga atgtgtagct gttgcctggg tttatggcgt tgataatttt tatgatgcga 240
ttgaggacat gattggctac agaccaaatc catggatgaa gtggagctgg actgttgtta 300
ctcctttact gtgcatgggt tgctttatct tctctttggt caaatacaag ccattaacgt 360
acaacaagat gtaccagtat cccgactggg ccattggaat cggctggact ctggccttgg 420
cgtccatgat ctgtatcccc atggttgtgg ttatcaagat cattcagtct gatgggccgc 480
tcattgagag gattaaggcg gtggcagctc cggttcgtgg cggggccagc tcccgtcccg 540
cggaccaccg tggccccaag gatgtcaccg taccgctgga ccccaacgga aacaagggtt 600
ttctgatgaa ggcccccacc cacaccatcg tagaaaccat gatgtaaagg aacgaggtgg 660
aggatctcca tgagatcgct ctcctctctt ctctgtcctc taaaaacgct tttactgcta 720
gctgtccgtt tgtctgtctt tctggtggac taaaaaaaga aaaaagggtt ttaaaggaaa 780
agttcacatt tcaaatcttt aaagaatacc ttggaagaaa gcctgcagtt tttggtcatc 840
aaaagtgggt ttagaaaaaa aataatccca ttttagatct gttagagctt ctagcaagca 900
gtttattttc tctctgttca tagtttcatg tgactttgca tcccacagtg cagcaacttg 960
gcaacaacat ctggtcatat gctggcagac ctgtttgtt 999
<210> 117
<211> 986
<212> DNA
<213> Oryzias javanicus
<400> 117
tccgctacag gggggtgttt gggactatca gtaccatggt ccgaacagag gggcccaggt 60
cgttgtacaa cgggctggtg gccggcttac aaagacaggt ctgctttgcc tccatcagaa 120
tcggactcta tgataacgtc aagaacttct acacaggcgg aaaggacaat ccaaacgtgc 180
tgattcggat actggccggc tgcacaacag gcgccatggc agtgtccttt gcacaaccca 240
cagacgtagt caaggttcga ttccaggccc agatgaacct gagcggtgtg gcccgccgat 300
acaacggcac gatgcaggcc tacaagcaga tcttccagaa tgagggcatg cggggcctct 360
ggaaaggaac gctacctaac atcacaagaa acgcgctggt taactgcaca gagctggtca 420
cgtatgacct gatcaaggag gccatcctcc gacaccgcct gctgtcagac aacctgccgt 480
gccactttgt gtccgcgttc ggtgctggct ttgttaccac ggtgattgct tccccagtag 540
atgtggtgaa aacgcgatac atgaactctc ctccgggaca gtacaggagt gccctcaact 600
gtgcatggac tatgatgacc aaagagggac cgacagcttt ttacaaaggg tttgtaccat 660
catttctgag gttgggatca tggaatgttg tgatgttcgt ctcatttgag caaattaagc 720
gcgccatgat ggtctcaagg caaaggattg aggtggcaaa ctgagactct tccccttcgc 780
ttccgttttc aacgttcaac tgccctctgg acacatttgc actttaaagc cagccgatgc 840
aacgaaacct gccagttata catgccatga agtggaaacc agtgcacatt ttattgacaa 900
tatttttaag aaaatgtaaa acaggtttct ataaattcta aatatatttg acatgtttta 960
acagaaaaaa aaaaaaaaaa aaaaaa 986
<210> 118
<211> 2040
<212> DNA
<213> Oryzias javanicus
<400> 118
ccttcctgaa gtcggctcca aagccagctc tgagaagaac cggagcctcc ttgctgtcgc 60
tggctgaaaa atgccccatc atcactcgcc agatcaccgt gagcggcccg gcctctctgg 120
actccaagct gagcagccct accagaacat ctaagggcaa gcagctttcc acggtggagc 180
aaaagaggca gtttgctcag accgccactc atgtggcggc gtccatgtcc aaggcctgcc 240
cctttgtttc ctctcagatc ggagtggtac aggccagttc tgaagtgcag gaggacgtgc 300
aggatggtct gatgaagtct ctgctgaaag gattaaaaga ttccatgttt ccatcaggaa 360
ctgacgtgaa caccgtcaca cacctcctca gagacaacat ggtgggtccc agctatgact 420
acgaccgctt ctttactgag aagatatctg agaaaaagaa ggaccacact tacagagtct 480
tcaagacggt aaaccgaagc gcagagacct ttccttttgc cgaggattac tccattcctg 540
ggagggcggg cgcccaggtg tccgtgtggt gcagcaatga ctatctggga atgagtcggc 600
accccaaggt tctcggtgcc atcagggaag ccctggagaa gtatggtgcc ggtgcaggtg 660
ggaccaggaa catctctgga accagcaact ttcacgtggc tctggagaga gaacttgctc 720
agctgcacag gaaagacgct gctttggtgt tctcctcctg ctttgtggca aacgattcca 780
cgctcttcac tcttgccaag atgctgccag ggtgcgagat ctactctgat gcagggaacc 840
atgcatcaat gattcagggg atcaggaaca gtggagccaa acgcttcatc ttccgtcaca 900
atgacaggcg acacctagag gagctgctgc agcgctctga ccccaagact cccaaaatag 960
tggcatttga gactgttcac tcaatggacg gtgccatctg tcctctggag gagctgtgtg 1020
atacggccca tcgttacggc gccctgacat ttgttgatga agttcatgct gttggcctgt 1080
atggagcaca cggagctgga gtgggcgaaa gagacaacat catgcacaag atcgacatag 1140
tttcagggac cttgggcaaa gcatttggtt gtgttggagg ttatgtggcc agcagcgccg 1200
ctctggtgga cacagttcgc tcctacgccg ctggattcat cttcacaacc gctttgcccc 1260
ccatggttct ggctggagcc ctggagtctg tccggatcct gaggagcgcc gaaggtcagg 1320
tgctccgaag agctcaccag aggaacgtga agcacatgag gcagctgctc atggacaagg 1380
gcttacctgt ggtcaactgc cccagccaca taattcctat tcgggttgga aacgctgagc 1440
tcaacacaaa ggtgtgtgac atcttgctgg agaaatacaa catctacgtg caggctatca 1500
actatcccac cgtgcctcgt ggtgaggagc tgctgcgcct ggctccatca ccgcaccacg 1560
agcctgatat gatggagtac tttgtgggga agctggtgga ggtgtggcgg gaggcaggtc 1620
ttcccctcaa cagtcctgcc accgccgcat gtacgttctg tgaccgccca ctccactttg 1680
acctcatgag tgagtgggaa aaatcctact ttggaaacat ggaaccccaa tatattactg 1740
tgtatgccta aaaccaaggt gtgttttggt ccaaagtgaa tctctgtgtg aaaaaaacat 1800
gtatgcactg attaaaaaca caaacattgt atttacagtt tccgatcctt tggctgcatg 1860
actcatttga tttagagttc tttttgcatt ctcagtggtg ttagtagaca tgaaaagttg 1920
ttttctttaa aagcatcaga cctgcacatt tgacactttc aaatctctta tccatgatag 1980
tttgaatgaa agtatttaat gttgctgatt tcatgtatta aagttaatta tggatgcttt 2040
2040
<210> 119
<211> 973
<212> DNA
<213> Oryzias javanicus
<400> 119
ggtattttgc actaagctca cagaggctgg aatccctcag gatgtcatta cagggatctt 60
ctccaacatc tcttcaatct actgcttcca cgataagttc ctgctcccgg agctcaagac 120
tcggattact ggagaatggg actccaaccc tcgcattgga gacattctcc agaaactggc 180
tccgttcatg aagatgtacg gggaatatgt gaagaacttt gaccgagcca tggacctggt 240
caacacctgg acgcagagat cctcacagtt caagagcgtc gttcagaaca tccagaaaca 300
ggatgtttgt gggaacctga cgttgcagca ccacatgctg gagccggtcc agcgcattcc 360
tcgctacgag ctgcttctca aagactacct gaaaaaactg cccagtgatg ctcctgacca 420
aaaagacgca gcaaaggcgc tggagctgat ctcaactgca gccaaccatt ccaacgcagc 480
catcaggaaa atggagaaaa tgcacaaact gttggaggtc tatgaaaggc tcggagggga 540
ggaagatata gtcaatccag ccaatgagct catcaaagaa gggcacatca agaagatgtc 600
agccaaaaat ggaacggcac aagatcgata tctgtacctg ttcaacaata tggtgctgta 660
ctgcgttcct aagctcagac tgatggggca gaagtttagc gtcagagaga ggatcgacat 720
tgctggcatg gaggttcaag agaacgtgaa gcaaaacctt ccccacacat ttgccatcat 780
tggtaaacaa cgttcactag agctacaggc caggacagcg gaggagaagg aagattggat 840
acaggtgatc atagccacca tcgaacggca caaacagaac agcgaaacct tcaataaagc 900
tttcatcagc tccttctccc gtgaggacga ccatgtgcca gactcaccgg gcccatggct 960
caactcatcc att 973
<210> 120
<211> 954
<212> DNA
<213> Oryzias javanicus
<400> 120
gggttcagcg tgttcttgtt aatggtgaat tatccccacc ttccatttgt aacactgggt 60
ctcctcaggg gtgtgtcctt tcccctctcc tttttatctt gtatacagac agttggagat 120
cttcagagga gaacagattt tttaatcaga ttctccaatg acactgttct agtgtctctg 180
ttatctgcat ctgaaattga ccacgggcct gctttgtcgc agtttgttga ttggtgtgat 240
gacaattacc tggatttaaa gtggagaaaa caaaggacat ggagattgat tttagacggg 300
ctacagcaca tggtgtcagt gtgatccatg gggagaaggt ggagactgtc gagagcttac 360
aaatatttag gcactgtatt cgacaacaaa ctgaaattcc acatgaacac agacgctatt 420
gtgaagcgtg ggcagcagag aatttacctg ctgaggaaac tgaactcttt taatgtgaaa 480
caaaccattc ttaatcattt ttattgttct tttattgaga gtattttaac attttcattt 540
atttgctggt tttatggtct gtctctgaaa gacaaaaaca aactcagagc attgtcaaga 600
cctgctccaa aataatcggt gttagtctga gggaccttcc ctctctgtgg aaggaacagg 660
tgctgaagaa agccaagaac atcctgggac agccccagca tcccctgact cctgactttg 720
ctttgatgcc ttcagggcgt cgattttatg ctccggtacg aaaaaccaat cgtttttcgc 780
agtcttttgt ggcctcagca attacgttgc tgaatttaga taaaagtgtt tttcttttga 840
ggtcattgct gaggtgacgt attgactgta tgctgatgtt acggagctat ggagcactca 900
ttttagactg tgattgtctg acacattaat atgattgtgt ggtgtgtgtg agaa 954
<210> 121
<211> 961
<212> DNA
<213> Oryzias javanicus
<400> 121
agcagtggta tcaacgcaga gtggagcggt acgaccctca cacgggacac tggacgagtg 60
tcacacccat ggccacgaag cgctcgggag ctggcgtggc tttgctcaac gaccacattt 120
acgtggtggg aggctttgat ggcatttcac atctggattc tgtggaggta tacaacatca 180
ggaccgacta ttggactact gtagccagta tgaccacacc tcgatgttat gtcggtgcaa 240
ctgtcctccg agggcgactt tacgccatag cagggtatga cgggaactct ctcctcagca 300
gtatcgaatg ttacgacccg gttctcgact cctgggaggt cgtcacttca atggcaaccc 360
agcgctgcga cgccggtgtc tgcgtccttc gagaaaagta gagctcacgt ggacaggagg 420
actttgctta catgcatcag agccacagag acgtatgtaa gacgctaaca gaagctcaga 480
taagtcaata aaaacgtgcc gcggctccgc cgccagagga tggtggggcc ttcttcagtc 540
aaactgccac agcatcacaa gagcccgtca ctaatgcatg actcttgggt ggtacctgtg 600
tctgtcatac ctgtgagatt attttttcag tgtcttcaaa gtgcaatata tttttactca 660
tcaagaaaaa aaaaaaaaga tggagcgggt ttagactcca gttgctgcta tacgccttac 720
atccaacatc aaacactcat gttgcctcga ttttactgac acttaaacac gactgaatgg 780
actcgatgtg acgtttacac gctcattatt gggttgaggg gtagaaaccc tgttgtgcat 840
ttcttcgcct gtaagcatta cttttgaatg gaaaaatcac tttattacag aaaaaaaatt 900
aatttcttaa aagttatatt caaagatgct tttagaaaga gtcgcatgtt atttcttttt 960
t 961
<210> 122
<211> 2147
<212> DNA
<213> Oryzias javanicus
<400> 122
aagcagtggt atcaacgcag agtggccgag gcggcctttt tttttttntt tttttttgat 60
ttgtctgtca tttttaattc atattttgga agcaaacagc tattttttcc aactctactc 120
actttattct ttattaactg agttatgtct gcacagctca tgtctcacac acacagtttt 180
gtctgaacac gtcagaaata caaatccctc agtacaaagt tcaataaaaa aaaaatcaat 240
ttaagagtaa aatgaacacc tgtgttgtaa atctattctt tttattcaaa aaaatctttg 300
aagtaaaaat gtaaatcatc tttctatttt aaggcacatt gactaaaaaa gagcagcaaa 360
ttccctcttt ttttaagtgt tcaaacactt tatggaataa aagttttttg acgcaactcc 420
agcgatgtca cacaggtcaa aaagtacaaa ctatcaacca caagagggca tgagttcaga 480
ttaagttaaa ttggtgcaga acaaaagcct cattttaatt gtcagtgaca atcccctcct 540
attttaatcc acatctaaat agaataaata tgtatctgcc aacataccat gtttacggct 600
ggtttcagtg gagccgcctc ctcatttcct gctcaaactc atttaattcc caaagctctt 660
caacaaacaa aagctgcagc tccaggtgga gtaatgctgc tccagactca gagtccataa 720
ggttcataca gaaggagtaa atgtttcacc aggacggcgt tgtgcaatag aatcaacaag 780
attacatgac ttctgattgt gacaattggc ctttcaccac ggatcaaaat tcagagcttc 840
tctgacactc aaacactcgg ctaaaaatca ccaggaatag tcaactttaa tctgcggaat 900
ccaaaatttc cagaataaaa gcgtaaaagg ttcacagaac taacttttca aacaatcaaa 960
tgtgatgcat gttgtccact gatatcactt tgatgctgtt tttcctatta gtgcatgaaa 1020
aaatgaaaga gagagtggtc tttacactgt caacatggat gctgcaaata aaggttacag 1080
aacggattta atgggcttgg gggtgttgtt cagaaaaaat ttatctgtga tgaacgtcgg 1140
caggtacgac atgggtagcc agaagaactt ggcgtcccat cctggggaat agcggtagcg 1200
ggggtgaaca gcagcaacgg cgtgctccat acagccgacc accttcatca ggtcaccgtc 1260
cgtaagctga ccgaaccgtt ggtccacccc ttcaagagtt tgctctagga aaacctttcc 1320
ataatcctcc tgcacatcct gaggtagtct gtcccagagc ttcttcaaat tgctcttcac 1380
taaggaagtg tcggtcacgc tggtcttgaa gaagcccggc tcgatgcaca gaactttaac 1440
tccgaatgga gccatgttca aacgtagaca gtcattaaag gcttccactc cgtacttgga 1500
cacacaataa ggtccaccaa atggactgat gcgcccgaac acactggcca cattaaccac 1560
tcggcctcta gccttcttga tgaggggaag gacgctcagc gttacgtcga tgactccgtt 1620
cagattgacc gccagcatag acttgaaatc ctctatgttg agccagtcat tgggaccgga 1680
tggaacactg accccagcgt tatttacaac cgcccacagg cctttctccc caactagagt 1740
cttgatgaaa gctgctgcct tgctaacact ttccgatttg gtaacatcca gggacacggt 1800
gttgagtctt tccgaggaga gcttcttcag ctgagtttca cccatctcgg tgtagcaggc 1860
tgcgatcacc cggaatccca gctggtctag atgatgcgcc agtttgttac cgaagccgga 1920
gtcgcagcct gtgatgtaga cgtatttgtc ccccttgttg ggaactctct tgatttcttt 1980
cagccatcgg tagatgaacc aaacggccac cagtgcgagg atgtaaagaa acattttcgc 2040
gtcttttcgt cgtcagtcag tcagtgcata aatcgtctct gtgggtggac aggaatcaca 2100
cgagtgtccc cggccgtaat ggccactctg cgttgatacc actgctt 2147
<210> 123
<211> 829
<212> DNA
<213> Oryzias javanicus
<400> 123
aagcagtggt atcaacgcag agtgaccatt acggccggga gggatctgtc tgaacagatc 60
aagaaagtta caaaggagag tcatgtcaga gcagaaaaca ccgagctcat gctcagcttc 120
cagcggggac aagtcacact tccacagtat aagctcctcc tgtgttccct gtacgagatt 180
tacaaagctc tggaggaaga gctggacagg aactccaacc atgctggtgt tgcacccatt 240
tactttccat ctgaactggc cagactggag tccattgaga aagacctgga gtatttctac 300
agcaccaact ggagggagaa gatcgttgtt cctgctgcta cgaagagata ctgtcacagg 360
atcagacaga ttggcaaaga gaacccagag tttctggtgg cccacgctta cacccgttac 420
ctcggcgatc tgtccggagg ccaggttctt ggtcggatcg cccagaaatc catggggctg 480
aaaaccagcg agggtctgtc tttcttcgcc ttccctggag tctccagccc caacctgttc 540
aagcagctct accgcagccg aatgaacagc gtggagctga cggaggagca gaggaacggc 600
gtgctggagg aggcggtccg gtccttcgag ttcaacatcc aggtctttga tgacatccag 660
aagatgctgg acgtcacaga aaacaagctg cagaacagtt taacagacaa gtcaagagag 720
gctaaaacgc tccacgtccc gctcatcagg atggtgctgg gactttttgt ggctctggct 780
acagtcagca tgggattcta cgctctataa aattagactt ttttttttt 829
<210> 124
<211> 812
<212> DNA
<213> Oryzias javanicus
<400> 124
aagcagtggt atcaacgcag agtgaccatt acggccggga tggacacggc gtacggcctg 60
gcgacgcact ggaggctgag gacggggcag gttcctgcca acaccgacag cgtgatgtgt 120
ttcgggcccc tggttcccga cggttacgcc gtctgctaca acccccaggc ggaccacgtc 180
cacttctcca tcaccgcctt caactgctgc gaggacacca acgcagagac gttatctcgc 240
accgtgaagg agactctgtg tgacctgcag gagctgctgg agcctcccgt gtagccccgc 300
ccccgccgcc gccagccagg aacaaaactg cttctaaaac ctttttgtac cgctttggtg 360
ctgtcgtgag aaccttttct tatgatcctg aaaagggctg atctggtggc ttttgaagtg 420
attttgatac tttttttaag gttctcatga agcagcgagt ctccgtcttt ctgcgagatc 480
agctcccgcg atggaagctg cagcgttttt gcggccgaag gcgacccgac actccagcct 540
tactcgggac aaaccgctgt cctctcaccg ctcaggacag aaccagccgc ctgcgaccgt 600
cccactaaga ataaatgatt tttacctctc ctgtgttggc tctttgtaga ttaaaaaagc 660
gactgaaagg accaaagagg acgaatacga acgagaaagt cttgaatttg ctgctttgct 720
ccaactgcct cagaattgag atgtttttgg atgtgtggcc ttgttgtcct ttcaacttgc 780
aggactggat aataaagtct gtgatcactt ag 812
<210> 125
<211> 804
<212> DNA
<213> Oryzias javanicus
<400> 125
atacagctga agtgaaaggt attgtggaac gattttgcga gagcacagcc cagcagagtg 60
aagagcttca ggcctggatg gagagactct taaaagcgtt ggagggggaa ctccatgcca 120
cctcaaacat gtctttttcg ttgcctgtgc aacacgacag ctctttgcag gaggactttt 180
ccgtgaagct gtctgctctg attcaggaca ttctgcactc tgtgcagtga caaaaacaca 240
catcgaagaa atgaagtttc aactcccgtc aaatcacttc attgacttac ctaacctgaa 300
ttcttgaatt cttctttttt tatatatata taactttacc atcaaaacaa agctgagtgt 360
aaatatttat aagatatttc tgaatgtttt ttttttctgt ctgcatgtga atgattgagg 420
atttgtgtct gtttcaatgt taaaacggtg ctaccttcta aaggcttctt tagtgatgaa 480
gccacataaa agttattttt caccaattta ccttcaagaa tatattttgt atatgcaaga 540
caacaatgca actctgaaag aatagttttt attcatcttt attatagaca gacaccaaat 600
gtttatatta gctcagaatt tctttcagca agatctgagt atatgtgcat attattgtaa 660
ataagtggta tgacaaatat aagctttacc aatgttattt gttacttttt aaaactagtg 720
caaagaaata aactatttta ctaaaacaaa aaaaaaaaaa aaaaaaaagg ccgcctcggc 780
cactctgcgt tgataccact gctt 804
<210> 126
<211> 720
<212> DNA
<213> Oryzias javanicus
<400> 126
aagcagtggt atcaacgcag agtgaccatc cacggcctca tctacaacgc tctgaagctt 60
ttcatggaga tgaatcagaa actctttgac gattgcacac agcagtttag ggcagaaaaa 120
aacaaagaaa aagctaaatc gaaggaacgt gaggaagctt ggatcaagat cgagaacctc 180
gctaaatcca acccacagtt ctttgtatat gttgattcct ctgacctcaa tagtccagta 240
gcaatggaga cggatgttcc tttgatagaa gatgttcaga ggttaaaaaa gacagttgag 300
gagggcgcaa ctcagctgca gcacgagcag cggaaagagc gggctatggt gcggcgcaag 360
tctgagttgc ctcaggatat ttacaccaca aaagccttgg agtcccaccg cagagttgaa 420
gaaatgttaa acacccacga cgggctctag gtcctaaaaa aaaaaaacaa aaaaaaactg 480
ccccttttta agctcaacag caatgggaca tgtctaaaaa ccaggctggg ctttccattc 540
ttcttcttcc cacctccagt tgtcctagaa ttcttagttc ttgttcattc ctgctggccc 600
accctcgatg cagaaaacat gtcgaagctc ctttttgatt tttctgcgtt ctcaggttct 660
tttagtcggc aatgagtgtg ttttgtgtgt ctttgcaaca tgctgttatt atttattctg 720
720
Claims (13)
- 서열번호 1 내지 126으로 기재되는 모든 유전자 각각의 핵산 서열의 올리고뉴클레오티드 전부 또는 그의 상보가닥 분자가 집적된, 바다송사리의 탄화플루오르옥탄술폰산염(Perfluorooctane sulfonate; PFOS) 노출 여부 검출용 마이크로어레이 칩(microarray chip).
- 제 1항에 있어서, 상기 유전자는 바다송사리(Oryzias javanicus)로부터 유래된 것을 특징으로 하는 탄화플루오르옥탄술폰산염 노출 여부 검출용 마이크로어레이 칩.
- 제 1항에 있어서, 서열번호 1 내지 58로 기재되는 유전자로 구성된 군으로부터 선택되는 유전자는 탄화플루오르옥탄술폰산염 노출에 대응하여 발현이 감소하는 것을 특징으로 하는 탄화플루오르옥탄술폰산염 노출 여부 검출용 마이크로어레이 칩.
- 제 1항에 있어서, 서열번호 59 내지 126으로 기재되는 유전자로 구성된 군으로부터 선택되는 유전자는 탄화플루오르옥탄술폰산염 노출에 대응하여 발현이 증가하는 것을 특징으로 하는 탄화플루오르옥탄술폰산염 노출 여부 검출용 마이크로어레이 칩.
- 1) 피검 시료에 노출된 실험군의 바다송사리와, 정상 대조군의 바다송사리에서 각각 RNA를 분리하는 단계;
2) 단계 1)의 실험군 및 대조군의 RNA로부터 cDNA를 합성하면서 실험군과 대조군을 각기 다른 형광물질로 표지하는 단계;
3) 단계 2)의 각기 다른 형광물질로 표지된 cDNA를 제 1항의 마이크로어레이 칩과 혼성화시키는 단계;
4) 반응한 마이크로어레이 칩을 분석하는 단계; 및
5) 분석한 데이터에서 제 1항의 마이크로어레이 칩에 집적된 유전자 발현 정도를 대조군과 비교하여 확인하는 단계를 포함하는, 시료내 탄화플루오르옥탄술폰산염(Perfluorooctane sulfonate; PFOS) 노출 여부 검출 방법.
- 제 5항에 있어서, 상기 시료는 생체시료, 식품시료, 화학시료, 공업시료, 임상시료 및 환경시료로 구성된 군으로부터 선택되는 어느 하나인 것을 특징으로 하는 탄화플루오르옥탄술폰산염 노출 여부 검출 방법.
- 제 5항에 있어서, 상기 단계 2)의 형광물질은 Cy3, Cy5, FITC(poly L-lysine-fluorescein isothiocyanate), RITC(rhodamine-B-isothiocyanate) 및 로다민(rhodamine)으로 이루어진 군으로부터 선택되는 어느 하나인 것을 특징으로 하는 탄화플루오르옥탄술폰산염 노출 여부 검출 방법.
- 1) 피검시료에 노출된 실험군의 바다송사리(Oryzias javanicus)와, 정상 대조군의 바다송사리에서 각각 RNA를 분리하는 단계;
2) 단계 1)의 RNA를, 서열번호 1 내지 126으로 기재되는 각각의 유전자에 상보적이고 유전자를 증폭할 수 있는 프라이머 쌍을 모두 사용하여 정량 실시간 RT-PCR(Quantitative real-time reverse transcript polymerase chain reaction, qRT-PCR)을 수행하는 단계; 및
3) 단계 2)의 유전자 산물을 대조군과 비교하여 발현 정도를 확인하는 단계를 포함하는, 시료내 탄화플루오르옥탄술폰산염(Perfluorooctane sulfonate; PFOS) 노출 여부 검출 방법.
- 제 8항에 있어서, 상기 시료는 생체시료, 식품시료, 화학시료, 공업시료, 임상시료 및 환경시료로 구성된 군으로부터 선택되는 어느 하나인 것을 특징으로 하는 탄화플루오르옥탄술폰산염 노출 여부 검출 방법.
- 제 1항의 마이크로어레이 칩을 포함하는 바다송사리의 탄화플루오르옥탄술폰산염(Perfluorooctane sulfonate; PFOS) 노출 여부 검출용 키트.
- 제 10항에 있어서, 스트렙타비딘-알칼리 탈인화효소 접합물질(strepavidin -like phosphatease conjugate), 화학형광물질(chemiflurorensce) 및 화학발광물질(chemiluminescent)로 이루어진 형광물질군으로부터 선택되는 어느 하나를 추가적으로 포함하는 것을 특징으로 하는 탄화플루오르옥탄술폰산염 노출 여부 검출용 키트.
- 제 10항에 있어서, 혼성화에 사용되는 완충용액, RNA로부터 cDNA(complementary DNA)를 합성하기 위한 역전사효소, dNTPs(deoxynucleotide triphosphates) 및 rNTPs(ribonucleotide triPhosphates, 사전 혼합형 또는 분리 공급형), 표식시약, 및 세척 완충용액으로 이루어진 반응 시약군으로부터 선택되는 어느 하나를 추가적으로 포함하는 것을 특징으로 하는 탄화플루오르옥탄술폰산염 노출 여부 검출용 키트.
- 서열번호 1 내지 126으로 기재되는 각각의 유전자에 상보적이고 유전자를 증폭할 수 있는 프라이머 쌍을 모두 포함하는 바다송사리의 탄화플루오르옥탄술폰산염(Perfluorooctane sulfonate; PFOS) 노출 여부 검출용 키트.
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| KR20140014642A (ko) * | 2012-07-25 | 2014-02-06 | 한국해양과학기술원 | 노닐페놀 노출에 대응하는 바다송사리 유전자 및 이를 이용한 수생태계 환경오염 진단 방법 |
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