KR101615900B1 - Composition containing Curcuma xanthorrhiza for preventing skin aging - Google Patents

Abstract

The present invention relates to a composition for preventing skin aging which contains Curcuma xanthorrhiza as an active ingredient, and more particularly to a pharmaceutical composition comprising an ethanol extract of Curcuma xanthorrhiza as an active ingredient, And cosmetic compositions. The present invention relates to a herbal medicine extract, which is free from adverse effects on the human body when ingested and prevents skin aging due to its photoaging inhibiting action, thereby having excellent skin improving effect such as wrinkle reduction and drying improvement, .

Curcuma xanthorrhiza, relieving wrinkles, improving drying, photoaging

Description

[0001] The present invention relates to a composition for preventing skin aging which contains as an active ingredient a curcuma nasal slicer. [0002] Composition containing Curcuma xanthorrhiza for preventing skin aging [

The present invention relates to a composition for preventing skin aging which contains Curcuma xanthorrhiza as an active ingredient, and more particularly to a pharmaceutical composition comprising an ethanol extract of Curcuma xanthorrhiza as an active ingredient, And cosmetic compositions.

Skin aging can be classified into intrinsic aging and extrinsic aging depending on factors that affect aging. Skin aging and aging can be divided into aging and aging, regardless of environmental changes. And exogenous aging is caused by continuous exposure to the external environment such as sunlight.

When ultraviolet rays come into contact with the surface of the skin, they are reflected by the surface of the skin, scattered by the nonuniform structure in the skin, or absorbed by the components constituting the skin such as melanin pigment. B ultraviolet rays and A ultraviolet rays exhibit different behaviors to skin. B ultraviolet ray is transmitted through the stratum corneum and skin surface to indicate erythema. When a large amount is received, blisters or inflammation accompanies pain, and A ultraviolet rays slowly show erythema It causes melanin to form melanin in the basal layer located at the lowest part of the epidermis, causing skin to darken and the color of skin changes to dark brown. Also, it is more permeable to skin than B-ultraviolet ray, The damage to the dermal tissue is expected.

In addition, sebum produced from epidermal cells forms a thin sebaceous membrane on the surface of epidermal cells, thereby preventing foreign matter from penetrating from the outside, protecting the skin from external stimuli, and lubricating the skin surface and inhibiting moisture evaporation. However, sebum secreted from the living body may cause lipid peroxidation due to ultraviolet rays during daily life and cause skin troubles. When the skin is exposed to ultraviolet rays for a long time, it causes pigmentation, skin troubles and aging of the skin. Therefore, it is necessary to pay close attention to skin damage due to ultraviolet rays.

Aging by sunlight is called photoaging, and ultraviolet light is a major cause of physiological and morphological changes in skin aging. As the aging of the endogenous skin progresses, the skin becomes dry, the fine lines increase, and the wrinkles deepen over time. And, due to the structural and functional changes of the epidermis, dermis, etc., the skin loses its elasticity and becomes sagged. While the thickness of the dermis decreases, the total amount of collagen (collagen) disappears 1% every year after becoming an adult, and the remaining collagen fibers become thicker and cross-linking increases, and the solubility, . In addition, the elastic fibers are thickened, and the crosslinking is also increased. In other dermis, the proliferation activity of fibroblasts is decreased, and the synthesis and resolution of collagen are also decreased. As such, collagen is a major skin tissue component related to skin aging. It is a protein that occupies 77% of the dry weight of the skin except the fat and 90% of the dermis fiber component. It functions to maintain the strength, elasticity and flexibility of the skin. . Therefore, the promotion of collagen synthesis and inhibition of decomposition has been a major concern in relation to skin cosmetic and anti-aging inhibition, and it is required to develop a cosmetic food that can enhance the synthesis of collagen while suppressing photoaging due to UV.

Curcuma xanthorrhiza is a traditional Indonesian medicinal plant, commonly known locally as temu lawak or Javanese turmeric, which is mainly composed of artumenone, a zingiberaceae plant, , Alpha-curcumene, beta-curcumene, curzerenone, germacrone, beta-sesquiphellandrene, alpha-toenone terpenoid compounds such as α-turmeone and xanthorrhizol, 7 to 30% essential oil, 30 to 40% carbohydrate and 0.02 to 2.0% (Lin SC et al., Am. J. Chin. Med. 23: 243-254, 1995).

The Curcuma xanthorrhiza is described in Korean Patent Laid-Open Publication No. 1999-0016507 as " a composition for treating acne and an antimicrobial agent for acne-inducing bacteria isolated from Curcuma nasalisers, " 0033751 discloses " a composition for oral hygiene comprising an extract of curcuma zotiri as an active ingredient as an active oxygen and nitric oxide production inhibitor ", and in Korean Patent Publication 2006-0053604, " Immune-enhancing polysaccharides and methods for their preparation ".

The present invention has been accomplished by confirming that the present invention is a medicinal plant and shows excellent effects of preventing wrinkles and improving skin care without causing side effects of natural herbicide Kerukuma.

Accordingly, the present inventors have conducted research to find out a plant-derived prescription showing the above-mentioned effect of improving the skin-beauty. More specifically, when the ethanol extract of Curcuma xanthorrhiza is taken as an active ingredient, The present invention provides a pharmaceutical composition, a health supplement, and a cosmetic composition excellent in prevention of aging of the skin due to photoaging inhibition action and alleviation of wrinkles and improvement in drying.

The present invention is intended to provide a pharmaceutical composition for preventing skin photoaging, which contains, as an active ingredient, Curcuma xanthorrhiza , a natural product, as a medicinal plant.

More particularly, the present invention relates to a pharmaceutical composition, a health supplement, and a cosmetic composition comprising an ethanol extract of Curcuma xanthorrhiza as an active ingredient.

Hereinafter, the present invention will be described in more detail.

The pharmaceutical composition for improving skin condition of the present invention contains Curcuma xanthorrhiza extract as an active ingredient. In addition to the Curcuma xanthorrhiza extract component, the pharmaceutical composition may further contain various additives, stabilizers and the like according to the required formulation. The Curcuma xanthorrhiza extract is extracted from fruit, leaves or roots of Curcuma xanthorrhiza , and alcohol is preferably used as an extraction solvent.

The method for producing Curcuma xanthorrhiza extract, which is a main component of the present invention, is not particularly limited, but a preferred method for producing Curcuma xanthorrhiza extract provided by the present invention is as follows.

After grinding the dried keruma jasgoriza, the extraction solvent is mixed and extracted. At this time, various extraction solvents can be used, but a 70% ethanol solution widely used in industry was selected and used. For example, when the above-mentioned 70% ethanol solution is mixed with the commutated egg yolk, the mixture is mixed at a ratio of 300 to 1000 ml of extraction solvent per 100 g of the sample. The temperature condition is 60 to 90 ° C. But it is generally extracted for 1 to 2 days with stirring, and then the ethanolic solvent is completely removed while concentrating the extract solution under reduced pressure to obtain 40 to 70 g of the final crude extract.

Hereinafter, how the active ingredient of the Curcuma xanthorrhiza extract adopted in the present invention changes in relation to the skin condition improving action without adverse effects on the human body during ingestion will be described.

The curcuma xanthorrhiza extract of the present invention was orally administered to mice for 5 days in total for 5 days at the same time for 12 weeks, and there was no difference in body weight between the groups. As a result, there was no difference in body weight, . Please refer to Fig.

In addition, damage to the stratum corneum of the skin by ultraviolet rays causes an increase in moisture evaporation in the epidermis, thereby lowering the moisture content in the epidermis. In order to determine the effect of the Curcuma xanthorrhiza extract on dryness by rapidly evaporating the lipid water due to UV-induced lipid metabolism abnormality and lipid deficiency in the skin, In the case of mice fed the extract of Curcuma xanthorrhiza , it was confirmed that the amount of water loss was small, so that Curcuma xanthorrhiza extract reduced the loss of transdermal water loss. See FIG.

The effect of skin thickness Curcuma xanthorrhiza extract, which is a physiological and morphological change of skin aging caused by ultraviolet rays, was examined. As a result, in case of a mouse ingested with Curcuma xanthorrhiza extract Was effective in inhibiting skin thickness reduction due to aging. See FIG.

Finally, in order to investigate the effect of the Curcuma xanthorrhiza extract of the present invention on photoaging symptoms, a skin replica was taken from a dorsal side of a hairless mouse before the autopsy with a silicone polymer , And the degree of wrinkling of the skin was compared with each other. As a result, it was confirmed that the wrinkles of the skin surface were alleviated. Please refer to Fig.

Specific uses of the Curcuma xanthorrhiza extract of the present invention include pharmaceutical compositions, health supplements and cosmetic compositions containing Curcuma xanthorrhiza extract, which will be described in detail below.

Curcuma xanthorrhiza according to the present invention is contained as an active ingredient in an amount of 10 to 80% by weight of the total pharmaceutical composition, preferably 15 to 50% by weight of the active ingredient.

The dosage of the pharmaceutical composition thus prepared may be appropriately adjusted depending on individual differences such as age, degree of lesion, form, and form, and may be appropriately adjusted to an effective amount of 0.01 to 1000 mg And the pharmaceutical composition of the present invention can be manufactured into a capsule, a tablet, a chewable tablet, a powder, a dry syrup, a granule, a chewing tablet, a soft capsule, a pill, a drink and a sour cream.

The pharmaceutical composition of the present invention may further contain a pharmaceutical adjuvant such as a preservative, a stabilizer, a wetting or emulsifying accelerator, a salt or buffer for controlling osmotic pressure, and other therapeutically useful substances, And the like.

Examples of formulations for oral administration include tablets, pills, hard and soft capsules, liquids, suspensions, emulsions, syrups, granules, etc. These formulations may contain, in addition to the active ingredient, diluents such as lactose, dextrose, , Mannitol, sorbitol, cellulose and glycine, lubricants, i.e., silica, talc, stearic acid and its magnesium or calcium salts, and polyethylene glycol. Tablets may also contain binders such as magnesium aluminum silicate, starch paste, gelatin, tragacanth, methylcellulose, sodium carboxymethylcellulose and polyvinylpyrrolidine, optionally mixed with starch, agar, alginic acid or its sodium salt Such as disintegrants, absorbents, coloring agents, flavoring agents, and sweetening agents. Tablets can be prepared by conventional mixing, granulating or coating methods.

It should be understood, however, that the actual dosage of the active ingredient should be determined in light of various relevant factors such as the severity of the symptoms, the route of administration selected, the age, sex, weight and health status of the subject.

Suitable dosages for the pharmaceutical compositions of the present invention are usually those skilled in the art that readily determine and prescribe effective dosages for the skin. The daily dose of the drug according to the present invention will vary depending on various factors such as the degree of progression of the subject to be administered, the age of onset, age, health condition, and complications, but in general, 1 to 500 mg / kg, preferably 30 to 200 mg / kg, can be administered once or twice a day, and the dose is not limited to the scope of the present invention by any means .

The present invention provides a health functional food containing Curcuma xanthorrhiza according to the present invention and a food-acceptable food supplementary additive, which comprises tablets containing Curcuma xanthorrhiza as an active ingredient, , A pill or a liquid preparation. The health food composition of the present invention contains Curcuma xanthorrhiza as an active ingredient in an amount of 0.001 to 10% by weight in the whole health food composition. Examples of food formulations include glucose, citric acid, A sugar, a margarine, a salt, a starch, a flour, a syrup, a maltose, a sugar, a sugar and a sugar ester in the form of a drink, Caramel, chocolate, diet bar, or snack foods.

The present invention provides a cosmetic composition for preventing skin aging comprising Curcuma xanthorrhiza according to the present invention as an active ingredient.

The cosmetic composition of the present invention contains Curcuma xanthorrhiza as an active ingredient in an amount of 0.001 to 10% by weight of the total cosmetic composition. The formulation of the cosmetic composition is not particularly limited and may be appropriately selected according to the purpose. For example, the cosmetic composition of the present invention can be used for cosmetics such as softening longevity, nutritional lotion, essence, nutritional cream, massage cream, pack, cleansing, foundation cosmetic foundation of body oil, mascara, eyeliner, makeup base and shampoo, A hair conditioner, a conditioner, or a hair cosmetic composition for a hair gel.

The composition for preventing skin aging according to the present invention can be easily prepared in the form of an extract or dried extract powder, and can be used for wrinkle removal, wrinkle prevention and elasticity enhancement. There is no particular limitation. In addition, since it is possible to obtain an effect of improving the condition of the skin even when applying the extract of Curcuma nigrescera to the skin or drinking it, it is easy to use as a component for improving the skin wrinkles of medicines, foods, cosmetics and foods.

Hereinafter, the present invention will be described in more detail by way of specific examples. However, it is to be understood that the present invention is not limited by the following examples, and that various changes and modifications may be made therein without departing from the spirit and scope of the present invention.

[Production Example 1] Curcuma xanthorrhiza ) Preparation of ethanol extract

After grinding the dried keruma jasgoriza, the extraction solvent is mixed and extracted. At this time, various extraction solvents can be used, but a 70% ethanol solution widely used in industry was selected and used. The 70% ethanol solution was added to the milled mixture. The mixture was mixed at a ratio of 500 ml of extraction solvent per 100 g of the sample. The temperature condition was 70 ° C. The extraction time may vary depending on temperature during extraction, Extraction was proceeded with slow stirring. The extraction solution was then recovered and the ethanol solvent was completely removed under reduced pressure. 40 to 70 g of the final microsomal crude extract obtained from the above step was obtained.

 [Test Example]

To investigate the effect of the composition of the present invention on photoaging symptoms, hairless mice were selected as animal models. Seven-week-old female mice (hairless mouse, SKH, HR-1) were divided into four groups of nine mice per group in a random manner on the basis of body weight of 1 to 20 days before oral administration. Table 1 below summarizes the animal test system.

In addition, the UV lamps were used for UV irradiation in four groups, and the amount of irradiation was confirmed using an ultraviolet light irradiator Waldmann UV meter. The first week was 1MED (55 to 60mJ / cm2), the second week was 2MED, the third week was 3MED and the last week was 4MED. UV irradiation was conducted at the same time three times a week from the start of the test to the end.

[Comparative Example 1]

The mice were randomly divided into groups according to body weight 1 day before oral administration of 20 to 25 g of a mouse (hairless mouse, SKH, HR-1) at 7 weeks of age. (Normal group).

 [Comparative Example 2]

The 7-week-old female mice (hairless mouse, SKH, HR-1) were randomly divided into groups based on body weight of 1 to 20 days before oral administration. (UV control group).

[Example 1]

20 to 25 g of the 7-week-old female hairless mouse (SKH, HR-1) were administered in a random manner on the basis of the body weight of 1 day before oral administration, Oral diet containing 20 mpk of extracts of extracted curcuma nigella extract was orally administered and UV irradiation was performed.

[Example 2]

20 to 25 g of the 7-week-old female hairless mouse (SKH, HR-1) were administered in a random manner on the basis of the body weight of 1 day before oral administration, Oral diet containing 100 mpk of extract of extracted curcuma nigella extract was orally administered and UV irradiation was performed.

Table 2 below shows the constitutions of Comparative Examples and Examples.

[Test Example 1] Weight change by confirming the safety of the extract of Curcuma nasalispora

In the above Preparation Example 1, the feed containing the extract of Curcuma longi extract extracted with 70% ethanol was administered at the same time for a total of 5 weeks and 5 days per week. Except for Comparative Example 1 from 2 weeks to 5 weeks after oral administration, Comparative Example 2, Example 1, and Example 2 were irradiated with UV light similar to sunlight three times a week. At this time, the total UV irradiation amount during the experiment was 600 mJ / cm ² .

The animal body experiment was carried out on the extract of Curcuma zanthoxylum prepared by the method of Preparation Example 1, and the change in body weight is shown in Fig.

As can be seen from FIG. 1, there was no difference in body weight among the animals in each of the comparative examples and the examples during the experiment period, and it can be seen that the curcuma nasalis extract of the present invention is safe without side effects on the human body when ingested with herbal medicine extract .

Test Example 2 Effect of Ultraviolet Radiation on the Transepidermal Water Loss (TEWL)

In the above Preparation Example 1, the feed containing the extract of Curcuma longi extract extracted with 70% ethanol was administered at the same time for a total of 5 weeks and 5 days per week. Except for Comparative Example 1 from 2 weeks to 5 weeks after oral administration, Comparative Example 2, Example 1, and Example 2 were irradiated with UV light similar to sunlight three times a week.

Percutaneous water loss was measured at 10 and 12 weeks using a vapormeter SWL4102 to determine the amount of percutaneous water loss in each experimental group. The environmental conditions at the time of measurement were relative humidity 60 ± 5% and temperature 25.5 ± 0.5 ℃. The results are shown in Fig.

From the results shown in Fig. 2, it can be seen that Comparative Example 2, which consumed the feed not including the curd jerker extract, had higher transdermal water loss whereas Comparative Example 2, which consumed the feed containing the curd jerky jerk extract, It can be confirmed that there is a significant difference between Example 2 and the transdermal water loss amount, and it was confirmed that the curcuma nasalisolate extract is effective on the transdermal water loss amount. . That is, skin moisturizing effect.

[Test Example 3] Influence of skin thickness on exposure to ultraviolet rays of the extract of Curcuma nasalisiana

In the above Preparation Example 1, the feed containing the extract of Curcuma longi extract extracted with 70% ethanol was administered at the same time for a total of 5 weeks and 5 days per week. Except for Comparative Example 1 from 2 weeks to 5 weeks after oral administration, Comparative Example 2, Example 1, and Example 2 were irradiated with UV light similar to sunlight three times a week.

The skin thicknesses of 10 weeks and 12 weeks of each experimental group were measured. The skin skin layer thickness was measured by scaling the H & E staining slide 100 times on a microscope and measuring the thickness of 10 randomly selected tissues per tissue to calculate the average value. The results are shown in Fig.

 From the results shown in Fig. 3, it can be seen that Example 1 and Example 2, in which the skin thickened in Comparative Example 2, in which the feed containing no curd jerk extract was consumed, while the feed containing the curcuma ja It can be confirmed that there is a significant difference in skin thickness between Comparative Example 2 and Skin Thickness, and it is confirmed that Kerusuke Nansoriza extract is effective on skin thickness.

[Test Example 4] was conducted to examine the effect of suppressing the photo-

In order to investigate the effect of the composition of the present invention on the photoaging symptom, the administration period of the feed containing the extract of Curcuma longi extract extracted with 70% ethanol in Preparation Example 1 was 5 times in total for 5 days for 5 days.

From 2 weeks to 5 weeks after the oral administration, UV, similar to sunlight, was irradiated to the group of Comparative Example 2, Example 1 and Example 2 three times a week. At this time, the total UV irradiation amount during the experiment was 600 mJ / cm ² . In order to determine the effect of wrinkle improvement, a replica of the skin was taken from the dorsal side of the hairless mouse before the autopsy. To compare the degree of wrinkles of the skin, Filed. The results are shown in Fig.

As can be seen from the results shown in Fig. 4, when the wrinkle-shaped image files were compared, the wrinkles of the skin surface of the hairless mice of Comparative Example 1, Comparative Example 2, Example 1 and Example 2 Was relaxed in Example 1 and Example 2 as compared with Comparative Example 2.

The pharmaceutical composition, health supplement and cosmetic composition containing the oral curcuma longish extract as an active ingredient can be applied to various formulations, but the present invention is not limited thereto.

[Pharmaceutical composition formulation example 1] Purification

The granules were formed by mixing 100 mg of the extract of Curcuma nasorrhiza, 50 mg of soybean extract, 50 mg of glucose, 50 mg of red ginseng extract, 96 mg of starch and 4 mg of magnesium stearate and 40 mg of 30% ethanol, followed by drying at 60 ° C., And then the tablet was tableted. The final weight of the contents is 400 mg.

[Formulation example 2 of pharmaceutical composition] Soft capsule

400 mg / capsule was prepared by mixing 100 mg of the extract of Curcuma juzo soja, 50 mg of soybean extract, 180 mg of soybean oil, 50 mg of red ginseng extract, 2 mg of palm oil, 8 mg of palm kernel oil, 4 mg of yellow radish and 6 mg of lecithin. Was prepared

[Pharmaceutical composition formulation example 3] Granule

The granules were formed by mixing 100 mg of the extract of Curcuma nasorrhiza, 50 mg of soybean extract, 100 mg of glucose, 100 mg of red ginseng, 50 mg of starch and 100 mg of 30% ethanol, followed by drying at 60 ° C. to form granules, Lt; / RTI > The final weight of the contents is 1 g.

[Health food composition formulation example 4] Drink

100 mg of the extract of Curcuma nasorrhiza, 50 mg of soybean extract, 10 g of glucose, 50 mg of red ginseng extract, 2 g of citric acid and 188 g of purified water were mixed and filled in a bottle. The final weight of the contents shall be 200 ml.

[Cosmetic Composition Formulation Example 5] Massage cream

Massage creams were prepared according to a conventional method by using a massaging cream formulation containing a curcuma varnish as an active ingredient of the Production Example (% by weight).

FIG. 1 is a graph showing a change in body weight with the safety of the extract of Curcuma nasalisporium.

2 is a graph showing the effect of inhibiting the transepidermal water loss (TEWL) of the curcuma nigrum extract.

3 is a graph comparing the effect of skin thickness on ultraviolet exposure of the curcuma nasalis extract.

FIG. 4 is a graph comparing the photoaging inhibition ability of the nasal cucumber extract with a skin replica.

Claims (8)

A cosmetic composition for preventing increase in thickness of skin skin layer by ultraviolet rays containing Curcuma xanthorrhiza extract as an active ingredient. The method according to claim 1, Wherein the Curcuma xanthorrhiza is obtained from an ethanol extract. 2. The cosmetic composition according to claim 1, wherein the curcuma xanthorrhiza is obtained from an ethanol extract. delete The present invention relates to a pharmaceutical composition for preventing skin thickness increase in skin caused by ultraviolet rays containing Curcuma xanthorrhiza extract as an active ingredient. 5. The method of claim 4, The Curcuma xanthorrhiza extract is an effective ingredient and contains 10 to 80% by weight of the total pharmaceutical composition. Curcuma xanthorrhiza ( Curcuma xanthorrhiza ) As an active ingredient, an active ingredient, an anti-diabetic health supplement that prevents the skin from increasing in thickness. delete delete

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