KR101605122B1 - Method for producing citrus fruits extract with high nobiletin or tangeretin content and use thereof - Google Patents

Abstract

The present invention relates to a method for producing citrus extract having high content of novelletin or a resorcinol. More specifically, citrus fruit is first washed with a basic solution and then extracted with an alcohol aqueous solution. The present invention relates to a method for producing an extract having a high content of retin and its use.
According to the present invention as described above, citrus extract having a high content of nobiletin or enamel retin is extracted by a simple method, and the cost is reduced. The present invention relates to a pharmaceutical composition containing a high-potency citrus extract having a high content of novirretin or tamsuletin as an active ingredient, for improving memory, dementia prevention, anti-cancer, antiinflammatory, atopic improvement, itching improvement, anti-obesity, lipid metabolic disease improvement, And to provide a composition and a pharmaceutical composition that have an effect of improving bone metabolism and improving fatty liver.

Description

TECHNICAL FIELD The present invention relates to a method for extracting a high-content extract of nobilisetin and enzymaticretin from citrus fruits,

The present invention relates to a method for extracting an extract having a high content of novelletine and resorcinol in citrus fruits and a use thereof.

Recently, various foods and pharmaceutical compositions using natural materials have been developed in order to reduce adverse effects and excessive stimulation when various chemicals are used as food or medicine. Natural materials have a low side effect and are highly popular with consumers, The value of development as a raw material is increasing.

Citrus fruits are a lot of fruit produced in Korea, and citrus fruits such as C.sunki hort. Ex Tanaka are cultivated in Jeju Island. For citrus fruits, vitamin C has been said to be synonymous. However, citrus has a variety of other functional materials that are beneficial to the body. Flavonoids, a polyphenolic compound with a small molecular weight, are a kind of this functional substance, and there exist about 60 kinds of citrus fruit. Most flavonoids have a hydroxyl group (-OH), which appears to be related to antioxidant functions. However, nobiletin belongs to a flavonoid group having a methoxyl group (CH3O-) among flavonoids, which is known to have a high biological activity even at a content lower than that of hydroxyflavone. In addition to nobiletin, there are also tangeretin and sinensitin. Especially, these ingredients are known to be found only in citrus fruits, so the usefulness of citrus fruits as a natural ingredient can be known.

Anti-cancer, anti-inflammatory, immunomodulatory, neuroprotective, and memory-restorative effects (Li, RW, Theriault, AG, Au) on citrus nobiletin, tangeretin and other polymethoxy flavonoids , K., Douglas, TD, Casaschi, A., Kurowska, EM and Mukherjee, R., Citrus polymethoxylated flavones to improve lipid and glucose homeostasis and modulate adipocytokines in fructose-induced insulin resistant hamsters. Life Sciences 79: 365-373, 2006) have been reported. In addition, the effect of improving liver function (Satomi Onoue et al., Physicochemical and biopharmaceutical characterization of amorphous solid dispersion of nobiletin, a citrus polymethoxylated flavone, improved hepatoprotective effects, European Journal of Pharmaceutical Sciences 49: 453-460, 2013) (Tsukasa Tominari et al., Polymethoxy Flavonoids (2004)). In addition, the present inventors have found that the use of the polymethoxy flavonoids , Nobiletin and Tangeretin, Prevent Lipopolysaccharide-Induced Inflammatory Bone Loss in an Experimental Model for Periodontitis, Journal of Pharmacological Sciences 119: 390-394, 2012), the effect of improving bone metabolic diseases including osteoporosis (Suguru Harada et al., Nobiletin, Polymethoxy Flavonoid, Suppresses Bone Resorption by Inhibiting NFκB-Dependent Prostaglandin E Synthesis in Osteoblasts and Prevents Bone Loss Due to Estrogen Deficiency, Jou (NF-κB, AP-1, and NF-κB) inhibited the action of histamine and the tangeretin ameliorate scratching behavior in mice (Sei Eun Jang et al., Rnal of Pharmacological Sciences 115: 89-93, 2011) and p38, International Immunopharmacology 17: 502-507, 2013), lipid metabolism abnormality improvement effect (Yuguang Lin et al., Molecular Structures of Citrus Flavonoids Determine their Effects on Lipid Metabolism in HepG2 Cells by Primarily Suppressing ApoB Secretion Journal of Agricultural, Chemistry 59: 4496-4503, 2011) have been recently reported.

Due to this pharmacological action, there is a growing demand for nonyltetin and benzetretin in citrus, and there is a growing need for a method for producing extracts having a high content at low cost from raw citrus fruits containing a large amount of nobiletin and resorcinol. Citrus fruits, especially C.sunki hort. Ex Tanaka, are one of 12 indigenous native mandarins native to Jeju. The dermis, which has dried the skin of the mandarin orange, has been used as a medicinal herb. C. hybrid Shiranuhi is a high-grade citrus fruit produced in Jeju. Jeju and Halabong have been reported to extract Novartin and Tangerine extract. Especially, it is necessary to effectively recycle these residues since the residues after the juice are left in large quantities when producing confectionery and beverage using hara ram.

In particular, nobiletin and tangeretin have been reported to be present in a large amount in the fruit juice but in the juice residue. Despite this possibility of the citrus juice residue, it has not yet been fully utilized as a food or medicine material, One of the reasons is the lack of an improved extraction method that economically obtains extracts containing a high content of Novartin and Benzaretin.

Korean Patent Laid-Open No. 10-2013-0034319 (a composition for preventing and treating obesity and hyperlipidemia containing an herbal extract of dandelion, cinnamon and cinnamon as an active ingredient) comprises at least one selected from the group consisting of Citrus unshiu, Angelica gigantis radix, The present invention relates to a pharmaceutical composition for the prevention and treatment of obesity, which comprises a mixed herbal medicine extract of Cinnamomi cortex as an active ingredient. In the prior art, the extraction method comprises the steps of extracting the skin of the orange with hot water, methanol, ethanol or a mixed solvent thereof Only. Thus, the conventional methods for preparing the extracts containing nobiletin and tamsuletin in citrus have various problems in industrial application due to low extraction efficiency, long time, high cost and high energy consumption. Accordingly, the present inventors intend to provide a novel method for extracting nobiletin and a high-content extract of retinol, and the use of the extract.

It is an object of the present invention to provide a novel method for extracting a high content of nobiletin or a marine retin in citrus fruits, which is a natural material without side effects, in comparison with the conventional extraction method.

It is also an object of the present invention to provide a composition containing citrus extract having a high content of novelletin or enzymaticretin as an active ingredient for improving memory, preventing dementia, anticancer, antiinflammation, atopic improvement, anti-obesity, improving lipid metabolism disease, A metabolic disease improvement and a fatty liver improvement effect.

In order to accomplish the above object, the present invention provides a method for producing citrus fruits, comprising the steps of: (1) washing and recovering flesh or peel of citrus fruits with a basic solution; (2) extracting the recovered product with an aqueous alcohol solution to obtain an extract; (3) filtering and then concentrating the extract to obtain a concentrate; And (4) precipitating the concentrate and drying to obtain a final product. The present invention also provides a method for producing a citrus extract containing Nobiletin or Tangeretin.

And a pretreatment step of finely pulverizing and washing the citrus flesh or skin before the step (1), and collecting and drying the citrus fruits.

The citrus fruits include citrus depressa, C. tachibana, C. leiocarpa, C. tardiva, C. succosa, C. kinokuni, C.erythrosa, C.deliciosa, C. nobilis, C.retuculata, C. tangerina, C.hanaya, And at least one citrus fruit selected from the group consisting of C. nippokoreana, C.sunki hort. Ex Tanaka and C. hybrid Shiranuhi.

The basic solution is prepared by adding a hydroxide (OH) compound containing at least one basic substance selected from the group consisting of sodium hydroxide, sodium bicarbonate, calcium hydroxide, potassium hydroxide, magnesium hydroxide and aqueous ammonia to distilled water at a concentration of 0.01 to 5% .

In the step (1), the basic solution is treated at room temperature for 20 hours or more.

In the step (1), the basic solution is treated at 50 to 80 캜 for 2 hours or less.

In the step (2), the alcohol aqueous solution is a 50-100% ethanol aqueous solution, the temperature for extracting with an alcohol aqueous solution is room temperature, and the time for extracting with an aqueous alcohol solution is 24 hours or more.

In the step (3), the filtered extract is allowed to stand at room temperature for 15 to 20 minutes and then concentrated.

In the step (4), the precipitation is performed by adding distilled water or 20% ethanol aqueous solution to the concentrate, and then centrifuging the precipitate to obtain a precipitate, followed by drying.

The present invention provides a citrus extract containing Nobiletin or Tangeretin extracted by the above-described method.

In addition, the present invention provides a pharmaceutical composition for improving learning ability or memory effect containing the above-mentioned citrus extract containing Nobiletin or Tangeretin as an active ingredient.

In addition, the present invention provides a pharmaceutical composition for preventing or treating dementia of old age, containing citrus extract containing nobiletin or tangeretin as an active ingredient.

The present invention also provides a pharmaceutical composition for anticancer therapy comprising the above-mentioned citrus extract containing Nobiletin or Tangeretin as an active ingredient.

The present invention also provides a pharmaceutical composition for improving antiinflammation, atopic eczema and skin itching, which comprises, as an active ingredient, a citrus extract containing Nobiletin or Tangeretin.

The present invention also provides a pharmaceutical composition for controlling anti-obesity and body weight, which contains the above-mentioned citrus extract containing Nobiletin or Tangeretin as an active ingredient.

In addition, the present invention provides a pharmaceutical composition for improving periodontal disease, which contains the above-mentioned citrus extract containing Nobiletin or Tangeretin as an active ingredient.

In addition, the present invention provides a pharmaceutical composition for improving bone metabolic diseases including osteoporosis, which contains, as an active ingredient, citrus extract containing nobiletin or tangeretin.

The present invention also provides a pharmaceutical composition for improving a lipid metabolism-related disease, which comprises the above-mentioned citrus extract containing Nobiletin or Tangeretin as an active ingredient.

The present invention also provides a pharmaceutical composition for the improvement of non-alcoholic fatty liver and the improvement of alcoholic fatty liver comprising the above-mentioned citrus extract containing nobiletin or tangeretin as an active ingredient.

In addition, the present invention provides a health functional food containing the above-mentioned citrus extract containing Nobiletin or Tangeretin as an active ingredient.

According to the present invention, citrus fruit or peel of citrus fruits is washed with a basic solution and recovered, and then extracted with an aqueous alcohol solution to extract a citrus extract having a high content of nobiletin or tamoxifen It is effective to reduce the cost.

The present invention also relates to a pharmaceutical composition comprising a nobiletin or a citrus extract having a high content of a resorcinol as an active ingredient and containing an effective amount of at least one compound selected from the group consisting of bone metabolism including osteoporosis, There is an effect of providing a composition having a disease improving effect and a fatty liver improving effect.

BRIEF DESCRIPTION OF THE DRAWINGS FIG. 1 is a process for preparing citrus extract having a high content of nobiletin or a margarine.
Figure 2 compares the PMF content with and without NaOH treatment.
Figure 3 compares the PMF content when treated with various concentrations of NaOH at 60 < 0 > C.
Figure 4 compares the PMF content of NaOH treated at various concentrations at room temperature.
Figure 5 compares the PMF content when the NaOH treatment time is varied.
6 compares the PMF content when the NaOH treatment temperature is varied.
7 compares the extracted PMF content when the concentration of the aqueous ethanol solution is changed.
Figure 8 compares the PMF content when ethanol concentration is varied during precipitation formation.
FIG. 9 is a view showing a state before the precipitate is recovered after the treatment of the detailed conditions of the method for producing citrus extract and the precipitation.

Hereinafter, the present invention will be described in detail.

(1) washing and recovering flesh or peel of citrus with a basic solution; (2) extracting the recovered product with an aqueous alcohol solution to obtain an extract; (3) filtering and then concentrating the extract to obtain a concentrate; And (4) precipitating the concentrate and drying to obtain a final product. The present invention also provides a method for producing a citrus extract containing Nobiletin or Tangeretin.

The method of the present invention may further include a pretreatment step of finely pulverizing and washing citrus fruits or peels prior to the step (1), followed by drying and recovering the citrus fruits.

The citrus fruits include citrus depressa, C. tachibana, C. leiocarpa, C. tardiva, C. succosa, C. kinokuni, C.erythrosa, C.deliciosa, C. nobilis, C.retuculata, C. tangerina, C.hanaya, C. nippokoreana, C.sunki hort. Ex Tanaka, and C. hybrid Shiranuhi. Among them, C.sunki hort. Ex Tanaka is one of the native mandarin oranges native to Jeju. C. hybrid Shiranuhi is a high-grade citrus fruit produced in Jeju. It is a kind of water that needs effective recycling.

The basic solution may be a hydroxide (OH) compound containing at least one basic substance selected from the group consisting of sodium hydroxide, sodium bicarbonate, calcium hydroxide, potassium hydroxide, magnesium hydroxide and aqueous ammonia, %, Preferably 1% by weight of the composition.

In the step (1), treatment of the basic solution at room temperature for 20 hours or more is most effective when treated at 60 ° C for 1 hour.

In the step (1), the basic solution is treated at 50 to 80 ° C, preferably at 60 ° C for 2 hours or less.

In step (2), the alcohol aqueous solution is 50 to 100% ethanol aqueous solution, the temperature for extracting with alcohol aqueous solution is room temperature, and the extraction time for alcohol aqueous solution is more than 24 hours.

In the step (3), the filtered extract is concentrated at 15 to 20 minutes at room temperature.

In the step (4), the precipitation is performed by adding distilled water or 20% ethanol aqueous solution to the concentrate, and then centrifuging the precipitate to obtain a precipitate and drying it.

The present invention provides a citrus extract containing Nobiletin or Tangeretin extracted by the above-described method.

In addition, the present invention provides a pharmaceutical composition for improving learning ability or memory ability by containing citrus extract containing nobiletin or tangeretin as an active ingredient.

The present invention also provides a pharmaceutical composition for preventing or treating dementia of the elderly, comprising a nobiletin or a citrus extract containing tangeretin as an active ingredient.

The present invention also provides a pharmaceutical composition for anticancer therapy comprising the above-mentioned citrus extract containing Nobiletin or Tangeretin as an active ingredient.

In addition, the present invention provides a pharmaceutical composition containing anti-inflammatory, atopic amelioration, and skin itching pharmaceutical compositions containing the above-mentioned citrus extract containing nobiletin or tangeretin as an active ingredient.

The present invention also provides a pharmaceutical composition for controlling anti-obesity and body weight, comprising the above-mentioned citrus extract containing Nobiletin or Tangeretin as an active ingredient.

In addition, the present invention provides a pharmaceutical composition for improving lipid metabolism-related diseases by containing the above-mentioned citrus extract containing Nobiletin or Tangeretin as an active ingredient.

The present invention also provides a pharmaceutical composition for the improvement of non-alcoholic fatty liver and the improvement of alcoholic fatty liver by containing the above-mentioned citrus extract containing nobiletin or Tangeretin as an active ingredient.

In addition, the present invention provides a pharmaceutical composition for improving periodontal disease, containing the above-mentioned citrus extract containing Nobiletin or Tangeretin as an active ingredient.

Also, the present invention provides a pharmaceutical composition for improving bone metabolic diseases including osteoporosis, comprising the above-mentioned citrus extract containing nobiletin or tangeretin as an active ingredient.

Examples of the carrier, excipient or diluent which can be used for the pharmaceutical composition in the present invention include lactose, dextrose, sucrose, sorbitol, mannitol, xylitol, erythritol, maltitol, starch, acacia rubber, alginate, gelatin, calcium phosphate, calcium silicate , Cellulose, methylcellulose, microcrystalline cellulose, polyvinylpyrrolidone, water, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate or mineral oil.

The pharmaceutical composition may be in the form of powders, granules, tablets, capsules, suspensions, emulsions, syrups, solutions, aerosols, excipients, injections, transdermal drugs or suppositories.

In the case of formulation, a diluent or excipient such as a filler, an extender, a binder, a wetting agent, a disintegrant, or a surfactant is usually used. Solid formulations for oral administration include tablets, pills, powders, granules, capsules and the like, which are prepared by mixing at least one excipient, for example, starch calcium carbonate, sucrose or lactose, Prepare. In addition to simple excipients, lubricants such as magnesium stearate and talc may also be used. Examples of liquid formulations for oral use include suspensions, solutions, emulsions, and syrups. In addition to water and liquid paraffin, which are commonly used simple diluents, various excipients such as wetting agents, sweetening agents, have. Formulations for parenteral administration include sterilized aqueous solutions, non-aqueous solutions, suspensions, emulsions, freeze-dried preparations, and suppositories. Examples of the suspending agent include propylene glycol, polyethylene glycol, vegetable oil such as olive oil, injectable ester such as ethyl oleate, and the like.

The present invention also provides a health functional food containing the above-mentioned citrus extract containing Nobiletin or Tangeretin as an active ingredient.

The health functional food may be prepared in the form of powders, granules, tablets, capsules, suspensions, emulsions, syrups, solutions, aerosols, extracts, tea, jellies or drinks. More specifically, dairy products such as drink, meat, sausage, bread, biscuit, rice cake, chocolate, candy, snack, confectionery, pizza, ramen, other noodles, gums and ice cream, various soups, beverages, alcoholic beverages and vitamins And includes all health foods in a conventional sense.

The citrus extract containing Nobiletin or Tangeretin according to the present invention can be added directly to the food or can be used together with other food or food ingredients and can be suitably used according to conventional methods. The amount of the active ingredient to be mixed can be suitably determined according to the purpose of use (prevention or improvement). Generally, the amount of the extract in the health food may be 0.01 to 15% by weight of the total food, and the health beverage composition may be added in a proportion of 0.02 to 5 g, preferably 0.3 to 1 g, based on 100 ml have. However, the amount added in the case of long-term intake intended for health and hygiene purposes or for the purpose of controlling health may be less than the above range, and since there is no problem in terms of safety, the active ingredient may be used in an amount exceeding the above range .

The health beverage composition according to the present invention is not particularly limited as long as it contains the above-mentioned extract at the indicated ratio as an essential ingredient, and may contain various flavors or natural carbohydrates as an additional ingredient such as ordinary beverages .

The functional cosmetic composition according to the present invention may be formulated into a flexible lotion, a convergent lotion, a nutritional lotion, a nutritional cream, a massage cream, an essence, a pack, a foundation, a lipstick or a powder foundation. In functional cosmetic formulations of the respective formulations, other ingredients other than the citrus extracts may be appropriately selected and mixed by those skilled in the art depending on the formulation or purpose of use of other cosmetics.

Hereinafter, the present invention will be described in more detail with reference to Examples. It is to be understood by those skilled in the art that these embodiments are merely illustrative of the present invention and that the scope of the present invention is not construed as being limited by these embodiments.

Examples. Preparation of Citrus Extracts

In the example of the present invention, citrus fruit or peel was cut, washed, recovered, and dried to prepare raw materials for extraction.

20 g of prepared citrus peel (in the experiment, citrus oranges) is treated with 200 to 400 ml of a 1% NaOH aqueous solution and allowed to stand at room temperature for 20 hours or more to weaken the texture of the citrus peel. Then, it was collected by filtration, washed with distilled water, and then extracted in 50 to 100% aqueous ethanol solution at room temperature for 24 hours or more. These results are the same as in Fig. Then, the insoluble component was filtered, and the filtered extract was concentrated at room temperature, distilled water was added, and the precipitate was separated by centrifugation at 3000 rpm for 3 minutes. After centrifugation, the precipitate is filtered, and then the distilled water or 20% ethanol aqueous solution is added to the filtrate. The precipitate is collected by centrifugation and dried to obtain the final citrus extract.

The following experiment was conducted to establish the process of producing such an extract.

Experimental Example 1. Measurement of PMF content by extraction condition

The content of Novartin and carboletin contained in the citrus extracts extracted by the above method was analyzed by high performance liquid chromatography (HPLC).

Detectors were analyzed by UV with wavelength of 215 nm. The mobile phases used in high performance liquid chromatography were analyzed with methanol and distilled water. The mobile phase conditions were analyzed with 70% (v / v) methanol. The analysis time was 60 minutes. The contents of novilectin and ergotretin were calculated after preparing a calibration curve using standard reagents. The standard reagent used was Wako's standard reagent. The concentrations of standard reagent used in the calibration curve were 0.01, 0.05, 0.1, 0.5, 1, and 5 mg / ml, and the concentrations of nobiletin and tamsuletin were analyzed using this calibration curve. In order to calculate the content, the concentration of each extract was fixed at 10 mg / ml and analyzed by high performance liquid chromatography. The content of Novartin and Triglyretin was calculated by using the weight of each extract as a denominator and the weight of each substance as a numerator using a calibration curve. The content of PMF was calculated by adding the contents of the contents of novirretin and ergotretin calculated in%.

Experiment result.

(1) Comparison of PMF content with and without NaOH.

The results of comparing the PMF content in the case of treatment with NaOH and in the case of not treatment in the extraction process according to the above example are shown in FIG.

As a result, when 1% NaOH was treated and 100% ethanol aqueous solution was used, the final extract had a content of PMF including nobiletin and resercinol of 50% or more. However, when treated with hot water at 60 ° C instead of 1% NaOH The PMF content was less than 20% and only slightly more than 20% when extracted with 100% ethanol and 50% ethanol without pretreatment of 1% NaOH solution. Therefore, in order to increase the PMF content, it is understood that the process of weakening the tissue of the citrus cell surface by treating with NaOH is essential and efficient.

(2) Comparison of PMF contents in the case of treatment with various concentrations of NaOH.

The results of comparing the PMF contents by changing the concentration of NaOH to 0.5%, 1% and 2% at 60 ° C. in the extraction process according to the above Example are shown in FIG. 3, and the concentration of NaOH at 0.01% 0.1%, 1%, and 5%, and the results of comparing PMF contents are shown in FIG.

As a result, PMF content was the highest when NaOH concentration was 0.5% at 60 ℃, but PMF content was the highest when NaOH concentration was 1% at room temperature. However, when applying high temperature treatment, additional equipment is needed for mass production. As a result, it is most effective to treat a basic hydroxide (OH) compound such as NaOH at a concentration of 0.01 to 5% by weight, preferably 1% by weight, in distilled water.

(3) Comparison of PMF contents when the NaOH treatment time was varied.

The results of comparing the PMF contents by varying the time for treating NaOH in the extraction process according to the above embodiment are shown in FIG.

As a result, it was found that the treatment at 1% NaOH concentration at room temperature for 24 hours or more was similar to the treatment at 1% NaOH concentration at 60 ° C for 1 hour, and that at 60 ° C 1% NaOH concentration, . When the NaOH concentration is 1% at the high temperature, the treatment is sufficient for 1 hour. When the NaOH concentration is 1% at the high temperature, the treatment is performed for 20 hours or more at room temperature.

(4) Comparison of PMF content in the case of changing the temperature during NaOH treatment.

The results of comparing the PMF content by varying the temperature for treating NaOH in the extraction process according to the above embodiment are shown in FIG.

As a result, although the PMF content was 20% or more at 50 to 80 ° C, the highest yield was obtained at 70 ° C. Since increasing the temperature increases the cost, it is most preferable to treat at 50 to 80 ° C for 2 hours, preferably at 60 ° C for 1 hour.

(5) Comparison of extraction yields when the ethanol concentration of the ethanol aqueous solution was changed.

The results of the comparison of the PMF contents by varying the concentration of the ethanol aqueous solution in the ethanol extraction process of the step (2) in the extraction process according to the above example are shown in FIG.

As a result, the highest yield was obtained when the ethanol concentration was 80%, and the useful PMF content was 50%. Considering the re-use of ethanol at the time of mass production, it is advantageous to have a high ethanol concentration. Therefore, it is most effective to use 50 to 100% ethanol aqueous solution, preferably 100% ethanol.

(6) The results of PMF content comparison with ethanol ratio during precipitation.

The results of comparing the PMF contents by different ratios of distilled water to ethanol in the ethanol precipitation process of step (4) in the extraction process according to the above example are shown in FIG. 8. The total amount of the raw material and the total amount of the precipitate is shown in Table 1 below.

As a result, in case of 50% ethanol ratio, the weight of the precipitate was small and the PMF content in the precipitate was only about 10%, but when the precipitate was formed using 20% ethanol, many precipitates were formed and the PMF content in the precipitate was 60% Good close figures were shown. When precipitated with distilled water, the content of PMF in the precipitate was close to 60%, but the weight of precipitate formation in the citrus raw material was as shown in Table 1. Therefore, it is effective to add distilled water or 20% ethanol aqueous solution to the concentrate in the precipitation process.

It was confirmed that the citrus extract prepared through the above examples contains nobiletin and cerzetine. Therefore, as described in the background of the present invention, it has been found that the anti-cancer, anti-inflammatory, anti- (Li, RW et al), liver function improvement effect (Satomi Onoue et al.), Anti-obesity effect (Young-Sil Lee et al.), Improvement effect of periodontal disease Tominari et al., Suguru Harada et al., Se-Eun Jang et al., And Yuguang Lin et al., Both of which have improved osteoporosis including osteoporosis. It can be used as a pharmaceutical composition containing the citrus extract as an active ingredient, a health functional food, and a functional cosmetic.

Having described specific portions of the present invention in detail, it will be apparent to those skilled in the art that this specific description is only a preferred embodiment and that the scope of the present invention is not limited thereby. It will be obvious. Accordingly, the actual scope of the present invention will be defined by the appended claims and their equivalents.

Claims (6)

(1) treating a basic solution of flesh or peel of citrus fruits at 50 to 80 DEG C for 0.01 to 2 hours and recovering,
Wherein the basic solution is added to a distilled water in an amount of 0.01 to 2% (w / v), in a distilled water, a hydroxide (OH) compound comprising at least one basic substance selected from the group consisting of sodium hydroxide, sodium bicarbonate, calcium hydroxide, potassium hydroxide, magnesium hydroxide, ) ≪ / RTI >;
(2) extracting the recovered product with an aqueous alcohol solution to obtain an extract;
(3) filtering and then concentrating the extract to obtain a concentrate; And
(4) precipitating the concentrate and drying to obtain a final product. ≪ Desc / Clms Page number 19 >
The method according to claim 1,
Prior to step (1) above,
A method for producing Nobiletin or Tangeretin-containing extract, which comprises pulping citrus flesh or rind and washing and drying.
The method according to claim 1,
The citrus fruits include citrus depressa, C. tachibana, C. leiocarpa, C. tardiva, C. succosa, C. kinokuni, C.erythrosa, C.deliciosa, C. nobilis, C.retuculata, C. tangerina, C.hanaya, Nobiletin or Tangeretin which is characterized by at least one citrus fruit selected from the group consisting of C. nippokoreana, C.sunki hort. Ex Tanaka and C. hybrid Shiranuhi. ). ≪ / RTI >
The method according to claim 1,
In the step (2)
Wherein the temperature for extraction with the alcohol aqueous solution is room temperature and the time for extraction with the aqueous alcohol solution is 24 hours or more.
The method according to claim 1,
In the step (3)
Wherein the concentrated extract is concentrated by allowing the filtered extract to stand at room temperature for 15 to 20 minutes and then concentrating the extracted extract with Nobiletin or Tangeretin.
The method according to claim 1,
In the step (4)
Wherein the precipitation step is performed by adding distilled water or an aqueous ethanol solution to the concentrate and then allowing the concentrate to stand to obtain a precipitate by centrifugation and then drying the precipitate.

Images