KR101575411B1 - Composition for controling pest comprising Nicotiana benthamiana extracts - Google Patents

Abstract

The present invention relates to a composition for controlling pests, and more particularly, to a composition for controlling pests comprising an extract of Nicotiana bentamiana as an active ingredient and a method for controlling pests using the composition. According to the present invention, Nicotiana < RTI ID = 0.0 > benthamiana ) extract showed that the tobacco had a repulsive effect on adults; The scattering inhibitory effect of tobacco budworm adults; The effect of cigarettes on the hatching rate of Louisiana; And tobacco are excellent in the insecticidal effect of adult rui. Accordingly, the composition of the present invention containing the nicotianabenthania extract of the present invention as an active ingredient can be usefully used for the insecticidal or repellent use of such a pest. In particular, the Nicotiana benthamiana extract of the present invention is a substance extracted from a plant, and thus has an advantage in that it can be environmentally-controlled for pest control, and it has no resistance problem because it has a different mechanism from that of the conventional chemical insecticide.

Description

(Composition for controlling pest containing Nicotiana benthamiana extracts) containing an extract of Nicotiana bentamiana as an active ingredient.

More particularly, the present invention relates to a composition for controlling pests comprising an extract of Nicotiana benthamiana as an active ingredient, and a method for controlling pests using the same.

Bemisia tabaci is a pest that seriously damages horticultural crops such as tomatoes, cucumbers and melons (Brown, JK et al., 2002. Adv. Bot. Res. 36, 65100; Jones, DR , 2003. Eur. J. Plant Pathol., 345, 428434; De Barro, PJ et al., 2006. Biol. Invasions 8, 287294). This pest is also a mediator that indirectly damages more than 200 plant viruses (Brown, JK et al., 2002. Adv. Bot. Res. 36, 65100). Recently, the damage caused by Louis-mediated Tomato Yalew leaf curl virus disease (TYLCV) has been spreading worldwide. In Korea, the viral disease was first detected in the tomato plantation area of Tongyeong in 2008 (Lee, H. et al., 2010. Mol. Cell, 30, 467-476). Although current tobacco has been chemically controlled using insecticides for the control of ruby, it has been difficult to control it due to the development of resistance to insecticides (Byrne, FJ et al., 1994. Bull. Entomol. Res. 84, 179184; Nauen, R. et al., 2002. Pest Manag. Sci. 58, 868-875). In recent years, the use of chemical pesticides has been restricted due to the rapid increase in organic farming sites. Therefore, development of environmentally friendly pest control methods other than chemical pesticides is urgent.

On the other hand, Nicotiana benthamiana is a commercially produced and marketed tobacco ( Nicotiana tabacum ) is the origin of Australia. Nicotiana botanicum plants have a large amount of grandual trichomes on the back of the leaves, which are present in a protruding form of the epithelium of the leaves. This structure is known to produce and secrete various compounds for the physico-chemical defense of plants from insects during evolution.

Recently, this plant has been widely used as a model plant in the field of plant molecular biology research. For example, hormone studies such as ethylene and various types of plant pathogens can be easily infected, so they are used in plant virus studies and are also used in the production of recombinant proteins (Goodin et al., 2008, Mol. Plant Microbe Interact., 21, 1015-1026).

However, there have been no studies on various physiological control efficacy and environmentally friendly powdery control agents of garlic using the extract of Nicotiana bentamidae.

Accordingly, the present inventors have made intensive researches to overcome the problems of the powdery insecticides. As a result, the present inventors have found that when tobacco is treated with Louisiana, nymphs and adults using an extract of Ventamiana plant, Scattering, and hatching) and insecticidal effects, and thus completed the present invention.

Korean Patent Publication No. 10-2013-0080667 Korean Patent Publication No. 10-2013-0048881 Korean Patent Publication No. 10-2013-0048890

Accordingly, an object of the present invention is to provide a composition for controlling pests which is eco-friendly and which can effectively insect or prevent Louis tobacco.

Another object of the present invention is to provide a pest control method which is eco-friendly and effectively enables the tobacco to insect or avoid Rui.

In order to accomplish the above object, the present invention relates to a method for producing Nicotiana benthamiana ) as an active ingredient.

In one embodiment of the present invention, the Nicotiana benthamiana extract can be extracted using water, C1-C4 lower alcohol or a mixture thereof as a solvent.

In one embodiment of the present invention, the lower alcohol may be ethanol.

In one embodiment of the present invention, the Nicotiana benthamiana extract may be extracted using a stem or leaf of Nicotiana bentamiana.

In one embodiment of the present invention, the Nicotiana benthamiana extract may be applied to at least one pest selected from the group consisting of Garuy, Spotted mite, aphid, Thrips worm, Brown planthopper, Chinese cabbage moth, Can have efficacy.

In one embodiment of the present invention, the powdered cigarette may be a Bemisia tabaci .

In one embodiment of the present invention, the Nicotiana benthamiana extract is a compound of the formula The scattering inhibitory effect of tobacco budworm adults; The effect of cigarettes on the hatching rate of Louisiana; And tobacco can have an insecticidal effect of adult rui.

The present invention is a cigarette comprising the step of processing the above-mentioned pest control composition to a crop Louis (Bemisia tabaci ).

In one embodiment of the present invention, the crop may be one selected from the group consisting of Chinese cabbage, lettuce, pepper, cucumber, tomato, eggplant, zucchini, melon and melon.

According to the present invention, Nicotiana < RTI ID = 0.0 > benthamiana ) extract showed that the tobacco had a repulsive effect on adults; The scattering inhibitory effect of tobacco budworm adults; The effect of cigarettes on the hatching rate of Louisiana; And tobacco are excellent in the insecticidal effect of adult rui. Accordingly, the composition of the present invention containing the nicotianabenthania extract of the present invention as an active ingredient can be usefully used for the insecticidal or repellent use of such a pest. In particular, the Nicotiana benthamiana extract of the present invention is a substance extracted from a plant, and thus it is advantageous in that it can be eco-friendly for pest control, and it has no resistance problem because it has a different action mechanism from the conventional chemical insecticide.

Fig. 1 is a view of a large-scale cultivation of Nicotiana bentamiana used in the present invention.
FIG. 2 shows an experiment conducted to analyze the effect of the Nicotiana benthamiana extract of the present invention on the insecticidal activity of adult rats.
FIG. 3 shows the results of analysis of the effect of the Nicotiana benthamiana extract of the present invention on the insect-kill rate (3a: water extract, 3b: ethanol extract).
FIG. 4 shows the results of analysis of the effect of the Nicotiana benthamiana extract of the present invention on the wet insecticidal rate of adult tobacco (4a: water extract, 4b: ethanol extract).
FIG. 5 shows the results of analysis of the effect of the Nicotiana benthamiana extract of the present invention on the emigration rate of tobacco budworm adults (5a: water extract, 5b: ethanol extract).
FIG. 6 shows the results of analysis of the effect of the Nicotiana benthamiana extract of the present invention on the induction rate of tobacco budworm adults.
Fig. 7 shows the results of analysis of the effect of the Nicotiana benthamiana extract of the present invention on the laying rate of tobacco budworm adults.
FIG. 8 shows the results of analysis of the effect of the Nicotiana benthamiana extract of the present invention on the hatching rate of tobacco.

The present invention relates to the use of Nicotiana < RTI ID = 0.0 > benthamiana ) extract as an active ingredient.

The " Nicotiana < RTI ID = 0.0 > benthamiana 'is a commercially grown and sold tobacco ( Nicotiana tabacum ).

The inventors of the present invention found that nicotianabentamiana extract inhibited the adherence of adult tobacco to tobacco; The scattering inhibitory effect of tobacco budworm adults; The effect of cigarettes on the hatching rate of Louisiana; And tobacco are excellent in the insecticidal effect of adult rui.

The extract of Nicotiana bentamiana according to the present invention can be obtained by extracting and isolating from nature using extraction and isolation methods known in the art, and the 'extract' defined in the present invention can be obtained by using an appropriate solvent And is extracted from Nicotiana bentamiana, for example, crude extracts of stem or leaves of Nicotiana bentamiana, polar solvent-soluble extracts, or non-polar solvent-soluble extracts.

As an appropriate solvent for extracting the extract from Nicotiana bentamiana, any pharmaceutically acceptable organic solvent may be used, and water or an organic solvent may be used, and examples thereof include, but are not limited to, But are not limited to, alcohols having 1 to 4 carbon atoms, acetone, ether, benzene (including benzene, toluene, xylene, and the like) including purified water, methanol, ethanol, propanol, isopropanol, Various solvents such as chloroform, ethyl acetate, methylene chloride, hexane, and cyclohexane may be used alone or in combination.

As the extraction method, any one of the methods such as hot water extraction method, cold extraction method, reflux cooling extraction method, solvent extraction method, steam distillation method, ultrasonic extraction method, elution method and compression method can be selected and used. In addition, the desired extract may be further subjected to a conventional fractionation process or may be purified using a conventional purification method. There is no limitation on the method for producing the Nicotiana benthamiana extract of the present invention, and any known method can be used.

For example, the nicotianabentamiana extract contained in the composition of the present invention can be obtained by subjecting the primary extract extracted by the above hot water extraction or solvent extraction method to a powder state by an additional process such as vacuum distillation, freeze drying or spray drying Can be manufactured. Further, the primary extract can be further purified by using various chromatographies such as silica gel column chromatography, thin layer chromatography, high performance liquid chromatography and the like, You can get it.

Therefore, in the present invention, the Nicotiana bentamiana extract is a concept including all extracts, fractions, and purified products obtained in each step of extraction, fractionation or purification, and diluted solutions, concentrates or dried products thereof.

The method of preparing nicotiana bentamiana according to an embodiment of the present invention can be manufactured by two methods as described below.

First, the extract of the present invention was prepared by adding 1.5 L of water or 50% ethanol solvent to 1 kg of nicotianabentamia powder obtained by harvesting and finishing the Nicotiana benthamiana leaf and stem, and precipitating it for 48 hours, Followed by lyophilization and pulverization of the solution.

In addition, leaves and stems of benthamiana were collected (1 kg) and were not crushed and precipitated in 1.5 L of water or 50% ethanol solution for 30 minutes. Then, the extract was separated using a filter, and the solution was lyophilized Followed by pulverization.

The Nicotiana benthamiana extract of the present invention produced by the above-described method can be applied to any one or more of the pests selected from the group consisting of Garuy, Spotted mite, Aphid, Thrips worm, Brown planthopper, It can have an insecticidal effect, and particularly, the tobacco has excellent insecticidal or repelling effect against Louis.

In one embodiment of the present invention, the Nicotiana benthamiana extract may be contained in an amount of 0.1 to 95% by weight based on the total weight of the composition for controlling insect pests.

The composition of the present invention may also contain, as an effective ingredient, a functional ingredient having a pest-suppressing use that has been conventionally used as long as the effect of the present extract is not impaired, in addition to the nicotienanthaxanamine extract described above. For example, conventional natural aroma extracts may include one or more herbal extracts such as rose geranium, rosemary, marzam, jasmine, peppermint, camphor, saxapras, lavender, thyme, pine, eucalyptus and basil extract , But is not limited thereto.

According to one embodiment of the present invention, the pest control composition may further comprise an agriculturally acceptable carrier.

The carrier may be inorganic or organic and of synthetic or natural origin, and the active composition may be mixed with or formulated with the carrier to facilitate the storage, movement and / or handling of the plant, seed, soil or other treated subject, Of the substance. In general, any material that can be routinely used as a carrier in pesticides, herbicides or antimicrobial compositions is suitable for use in the present invention. The natural pest control compositions of the present invention may be used alone or in combination with other active ingredients such as the solid and / or liquid dispersible carrier vehicles and / or other compatible compatible actives, especially other insecticides, For example, in the form of a mixture with plant protection agents such as nematicides, fungicides, bactericides, bactericides, rodenticides, herbicides, fertilizers, growth regulators and the like, It can also be used in ready-to-use forms such as solutions, emulsions, suspensions, powders, pastes and granules. The natural pest control composition of the present invention may optionally contain conventional inert (plant compatible or herbicidally inert) insecticide diluents or swelling agents of the type available for conventional insecticidal agents or compositions, for example, conventional gas , Natural or synthetic materials, microcapsules, seeds incorporated with active compounds, solutions, emulsions, suspensions, emulsifiable concentrates, spray powders, pastes, soluble powders, dust collectors, granules, foams, pastes, tablets, aerosols, A coating composition to be used and a preparation for use with burning equipment such as a foaming cartridge, foamable can and foaming coils, as well as ULV cold air fog and hot air fogging agents, and the like. It is preferable that the carrier is adjusted according to the formulation, spraying location and spraying method of the composition for controlling natural pest.

The present invention also provides a pest control product (e.g., an insecticide or repellent) comprising the above composition.

The pest control product may be one formulated in a suitable form depending on the purpose and use of the pest. For example, the pest control product may be a granule, a powder, a liquid, an aerosol, a spray, an extract, a paste, a fluid, The composition may be a capsule, a liquid wettable powder, a granule wettable powder, a wettable powder, a powder, a fine particle, an oil, a gel broth, a fuming agent, a fumigant and the like. desirable.

The present invention also provides a pest control method comprising the step of applying a pharmaceutically effective amount of the pest control composition to a crop.

The pests may be any one or more of pests selected from the group consisting of powdered mites, spotted mites, aphids, thrips, brown planthoppers, cabbage moths, and tobacco marsupials, but the kind thereof is not particularly limited.

The pest controlling composition of the present invention may be used in formulations such as aerosols, pump sprays, liquids, suspensions, sprays, capsules, paints or gels, but is not limited thereto. As the spraying agent, LPG, n-butane, isobutane, propane, carbon dioxide, Freon, HCFC, HFC, nitrogen and oxygen may be used alone or in combination. The spraying agent for the aerosol formulation may be at least one selected from liquefied natural gas and dimethyl ester, compressed air, compressed nitrogen, wherein the ratio of composition to propellant is in the range of 65: 35 to 40:60 by volume But is not limited thereto.

The pesticidal composition composition of the present invention may contain a volatile solvent in combination with the nicotiana bentamiana extract. The volatile solvent is preferably ethanol or methanol, but is not limited thereto.

The composition for controlling pests of the present invention can be used both as a food poisoning agent or as a contact poison, and is preferably used as a contact poison agent to enhance insecticidal effect, but is not limited thereto.

In order to prevent such a phenomenon, the insect pest control composition of the present invention is applied to the insect pest control composition of the present invention in order to prevent the insect pest from infiltrating into the body by allowing water droplets to flow by using surface tension when the liquid component comes into contact with the surface thereof. It is preferable to mix the penetrating agent.

The composition for controlling insect pests may be diluted to an appropriate concentration at the time of use.

The diluting solvent may be at least one selected from the group consisting of water, alcohol, and excipient. For example, water may be used.

The pest control method may be applicable to a method of directly spraying or applying the composition for controlling insect pests on crops and / or soil in the facility, or fumigation disinfection. Particularly when fumigation or smoke is used, it is appropriate to use the amount of about 30 to 120 mg per unit area (m 2) and about 50 to 120 mg (m 3) per unit space based on the amount of active ingredient. However, the use amount thereof is not limited to the above range since it is applied differently depending on the growing condition and size of the crop.

The insecticidal crop according to the present invention is not particularly limited and includes at least one species selected from the group consisting of Chinese cabbage, lettuce, pepper, cucumber, bean, chicory, tomato, eggplant, pumpkin, paprika, melon and melon .

Hereinafter, the present invention will be described in more detail with reference to Examples. It will be apparent to those skilled in the art that these embodiments are merely illustrative of the present invention and that the scope of the present invention is not limited to these embodiments.

< Example  1>

Nicotiana Ventamiana ( Nicotiana benthamiana Mass cultivation and extraction of active ingredient

&Lt; 1-1 > Nicotiana Ventamiana  Extract preparation

The Nicotiana benthamiana used in the present invention was cultivated in Chungbuk Agricultural Research Institute (see Fig. 1). Extraction of active components was carried out using two solvents in two different ways.

First, Nicotiana benthamiana leaf and stem were collected, dried and then ground to make powder. Then, 1 kg of the milled bentamiana powder was precipitated with water or 50% ethanol solution (1.5 L) at 25 ° C for 48 hours and then extracted with a filter. Second, Nicotiana benthamiana leaves and stems were collected (1 kg) and were not crushed, and they were precipitated in 1.5 L of water or 50% ethanol solution for 30 minutes and then extracted with a filter. The extracted solution was separated by using a filter and then freeze-dried to obtain a final product.

< Example  2>

Nicotiana Ventamiana  Extract The cigarette is Louis  Insecticidal efficacy test method for adults

In order to investigate whether the Nicotiana benthamiana extract of the present invention prepared in Example 1 exhibits an insecticidal effect against adult Lui, the following experiment was conducted.

Tobacco produced a Louis artificial feeding chamber (see Fig. 2). The glass tube (diameter 3 cm, height 12 cm) was sealed with two layers using a parafilm on the top. The lower part was sealed with plastic, and a hole (0.5 cm in diameter, 2 cm in height) was pierced using a plastic tube in the center. The tobacco used 20% sucrose, an artificial diet of Louis. The Nicotiana benthamiana extract of the present invention prepared in Example 1 was mixed with sugar water to make 0, 1, 10, and 20%, and then about 200 μl was added between the two-folded para-films on the upper side of the glass tube . Through the plastic tube hole at the bottom of the glass tube, the cigarette put the adult Lu (50) and closed the hole with cotton. The tobacco using the glass tube was observed in the Louis juice experiment. The live adult tobacco adhered to the upper parafilm membrane to juice the sugar water, but the dead tobacco powder fell to the bottom floor. After 12 hours of treatment, the rate of tobacco smoke was examined. A cigarette that feeds artificial food (sugar water) between the parafilm membranes is a form of adult rui (Fig. 2).

< Example  3>

The Nicotiana Ventamiana  Extract The cigarette is Louis  imago Incidence rate

In this experiment, the extracts of the nicotienabenthamiana extract according to the present invention prepared in Example 1, which were prepared by the second method (water or 50% ethanol) And investigated whether they could induce insect pests.

In detail, the selective induction rate and the insecticidal rate of tobacco by the time lapse (24, 48, 72, 96 hours) were compared using a cage in which the tomato and the Nicotiana benthamiana extract of the present invention were combined.

As a result, as shown in FIG. 3A, it was confirmed that the tobacco powder was induced 53.9% and 23.5% in the tomato and the nicotianan bentamiana extract of the present invention after 24 hours, respectively, and this ratio was 96 hours Similar afterwards. On the other hand, as shown in FIG. 3B, the insecticidal rate of the tobacco plants attracted to each plant was lower than 10% even after 60 hours in the tomatoes as shown in FIG. 3B. However, the extract of Nicotiana bentamianae 79.8% of the attracted powder was found to be insecticidal. In addition, a comparative test of N. tabacum plant extracts similar to the Nicotiana bentamiana extract of the present invention showed that the insecticidal rate was 21.5%.

Namely, the tobacco powder attracted to the extract of Nicotiana benthamiana of the present invention was found to be almost dead. These results indicate that the nicotiana antimony extract of the present invention attracts powdery mushroom, unlike tomato and tobacco, Prove that you have.

< Example  4>

The Nicotiana Ventamiana  The extract The cigarette is Louis  Adult On the wet insecticide rate  Impact

In this experiment, the extracts of the nicotiana bentamiana extract of the present invention prepared in Example 1, each of the extracts prepared by the second method (water or 50% ethanol) The effects on the wet insecticidal rate were examined.

In detail, in order to measure the wet insecticidal rate of the Nicotiana benthamiana extract of the present invention, about 200 μl of the extract of the present invention was added to the cage made of 0, 0.5, 1, 10 or 20% by mixing with the sugar water And the tobacco budworm larvae were killed for 84 hours.

As a result, as shown in FIG. 4, the insecticidal rate after 84 hours was 21% in the control water treated with sugar water (20%), whereas in the test group treated with the nicotianan bentamiana extract of the present invention, Water or ethanol), the mortality was 100% at the concentration of 10% or more, and 58-62% of the water and ethanol extract at the concentrations of 0.5 and 1%. 4a shows the water extract of Nicotiana bentamiana according to the present invention, and FIG. 4b shows the ethanol extract of Nicotiana tabacumina according to the present invention.

< Example  5>

The Nicotiana Ventamiana  The extract The cigarette is Louis  Adult On the allegorical rate  Impact

In this experiment, the extracts of the nicotiana bentamiana extract of the present invention prepared in Example 1, each of the extracts prepared by the second method (water or 50% ethanol) And the influence on the allegorical rate.

In detail, the extract of the present invention was mixed with sugar water to make 0, 1, 10, and 20% of the extract of the present invention in order to comparatively analyze the emergence rate of adult tobacco, (24, 48, 72, 96, 120, 144, 168 hours) according to the number of adults.

As a result, as shown in FIG. 5, a total of 62.5 birds were alienated at 7 days after spraying the leaves on the untreated control, whereas 51.0% of the ethanol extract of the present invention (20% (31.9), respectively. However, no difference was observed between the control and the other concentrations. For reference, FIG. 5A shows the water extract of Nicotiana bentamiana of the present invention, and FIG. 5B shows the ethanol extract of Nicotiana tabacumina of the present invention.

That is, the cigarette yield of Louisiana was found to be efficacious in bentamiana and 20% ethanol extracts, but not in water extracts. These results suggest that the extracts extracted with two different solvents have different components that inhibit the algae 's allegation.

< Example  6>

The Nicotiana Ventamiana  The extract The cigarette is Louis  Influence on adult rate of adult

In this experiment, the effect of the ethanol extract prepared by the second method on the induction rate of tobacco budworm adults was investigated as the nicotiana antimony extract of the present invention prepared in Example 1 above.

In detail, in order to compare the incidence rate of adult tobacco to tobacco, the extract of the present invention was mixed with sugar water to make 0, 1, 10, and 20%, and the leaf was sprayed on the leaves of the tomato with garnet nymph. (12, 24, 36, 48, 60, 72, 84 hours) according to the test.

As a result, as shown in FIG. 6, it was confirmed that the attracting rate of the powder to the tomato was remarkably increased as the concentration of the nicotienabantamia extract of the present invention was increased. The incidence rate was 20% of the untreated at 20% concentration at 12 hours after treatment, and the rate of decrease in incidence rate was similar to that of the first at 84 hours. That is, the Nicotiana benthamiana extract of the present invention exhibited a high avoidance rate to adult tobacco.

< Experimental Example  7>

The Nicotiana Ventamiana  The extract The cigarette is Louis  Adult On egg production  Impact

In this experiment, the extracts of the nicotiana bentamiana extract of the present invention prepared in Example 1, each of the extracts prepared by the second method (water or 50% ethanol) The effects on egg production were examined.

In detail, tomato leaves were precipitated in two kinds of Nicotiana bentamiana extract (water or ethanol) and then dried. The number of eggs laid after the administration of a pair of cigarettes Louis and the male on the leaves was compared for 9 days.

As a result, as shown in FIG. 7, the number of eggs scattered in the non-treated area was about 72 per female, while the number of eggs scattered in the leaves precipitated in the nicotiana bentamiana extract of the present invention was inversely proportional to the concentration . In other words, the number of spawning was 36.6 and 33.3 at 20% concentration of water and ethanol extracts, which was 46.7% and 50.7% of the control, respectively. From the above results, it was found that both of the extracts of benthamiana suppressed the scattering of powdery matter to about 50% level.

< Experimental Example  8>

The Nicotiana Ventamiana  The extract The cigarette is Louis  Effect on hatching rate of eggs

In this experiment, the extracts of the nicotienabenthamiana extract of the present invention prepared in Example 1, which were prepared by the second method (water or 50% ethanol) Hatching rate.

In detail, the extract of Nicotiana benthamiana of the present invention was sprayed on the egg with the tomato leaf. The eggs were used for about 100 days per sample as eggs for the second day after egg laying.

As a result, as shown in FIG. 8, the hatching rate of the eggs in the untreated sample was 92.1%, while the hatching rate of the present invention was significantly decreased when the concentration of the treated nicotiana bentamiana extract was higher . That is, when the extract of Nicotiana bentamiana was treated with 20% concentration, the hatchability of eggs decreased to 7.9% for water extract and to 3.5% for ethanol extract. It was found that the hatching rate was significantly suppressed as 9.1% and 3.6% of the control.

The present invention has been described with reference to the preferred embodiments. It will be understood by those skilled in the art that various changes in form and details may be made therein without departing from the spirit and scope of the invention as defined by the appended claims. Therefore, the disclosed embodiments should be considered in an illustrative rather than a restrictive sense. The scope of the present invention is defined by the appended claims rather than by the foregoing description, and all differences within the scope of equivalents thereof should be construed as being included in the present invention.

Claims (9)

Nicotiana benthamiana ) as an active ingredient. The method according to claim 1,
Wherein the nicotiana antimony extract is extracted using water, C1 to C4 lower alcohols or a mixture thereof as a solvent. 3. The method of claim 2,
Wherein the lower alcohol is ethanol. The method according to claim 1,
Wherein the nicotianan bentamiana extract is obtained by using a stem or a leaf of Nicotiana bentamiana. The method according to claim 1,
Wherein said Nicotiana botanicumiana extract has insecticidal activity against at least one insect pest selected from the group consisting of powdery mildew, spotted mite, aphid, insect bug, brownfly, cabbage moth, / RTI &gt; 6. The method of claim 5,
Wherein the powdery tobacco is Bemisia tabaci . The method according to claim 1,
The Nicotiana panthera mugwort extract may be used to prevent the tobacco attack of adult rui; The scattering inhibitory effect of tobacco budworm adults; The effect of cigarettes on the hatching rate of Louisiana; And the tobacco has an insecticidal effect of adult rats. To claim 1, wherein the first cigarette comprising the step of treating any one of the pest control composition of 7, wherein the crop Louis (Bemisia tabaci) control methods. 9. The method of claim 8,
The crops of tobacco, wherein one member selected from the group consisting of cabbage, lettuce, peppers, cucumbers, tomatoes, eggplant, zucchini, melons and melons Louis (Bemisia tabaci) control methods.

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