KR101565159B1 - Compositions for preventing or treating aging and arthritis - Google Patents

Abstract

The present invention relates to a composition for preventing or treating aging and arthritis, and more particularly, to a composition for preventing or treating aging and arthritis, which comprises extracts of chick, honey, dermis, Angelica keiskei, Aging, and arthritis.
The composition according to the present invention has an excellent effect in inhibiting hyaluronidase activity and skin wrinkles and increasing skin elasticity, bone mineral density and elasticity.

Description

[0001] The present invention relates to a composition for preventing or treating aging and arthritis,

The present invention relates to a composition for preventing or treating aging and arthritis, and more particularly, to a composition for preventing or treating aging and arthritis, which comprises extracts of chick, honey, dermis, Angelica keiskei, Aging, and arthritis.

Aging refers to all the physiological changes of the body that occur over time. It is a life phenomenon that occurs in a wide variety of ways depending on the individual. It is associated not only with the degeneration of cells, tissues and organs that lead to many diseases such as cancer, cardiovascular insufficiency, obesity, and non-alcoholic fatty liver, but also with the weakening of most physiological performance limits. It is difficult to study aging at the individual level, since aging varies in appearance and speed from individual to individual and varies from organization to organization. On the other hand, when the aging phenomenon is specifically examined, the functional changes of the constituent organs and tissues occur, which is caused by a change in the function of the constituent cells. In other words, whether the cognitive function is lowered due to brain nerve cell loss or the skin elasticity is reduced due to the disappearance of subcutaneous adipocytes, or the hair is whitened by the loss of melanin pigment production ability of the hair follicle melanin cells, Of the cells.

On the other hand, arthritis is a chronic disease afflicted by 5% of the population of Korea. It causes inflammation in joint synovial membrane, which causes edema and pain. Arthritis is a progressive disease and causes joint deformity and disability and continues to worsen if left untreated, with serious consequences.

The direct causes of arthritis have not yet been clearly elucidated. For the treatment, steroids such as cortisone and other corticosteroids, non-steroidal anti-inflammatory drugs such as aspirin, piroxicam and indomethacin, Anti-rheumatic agents such as D-penicillamine, antifungals such as colchicine, and immunosuppressants such as cyclophosphamide, azathioprine, methotrexate and rebamisole are commonly used. However, these chemotherapeutic agents do not exhibit fundamental therapeutic effects, and steroid hormones are limited in their use due to side effects. In addition, aspirin agents and botazoline agents, which are widely used as agents for alleviating joint pain and eliminating inflammation, have a fatal effect on humans, and it is impossible to continuously take the necessary amount to treat arthritis.

These conventional chemotherapeutic agents have disadvantages such as side effects that make long-term use of medicines difficult, and decrease of anti-inflammatory effects in long-term use. At present, indomethacin and furupenic acid, which are excellent in analgesic action, and various non-steroidal anti-inflammatory agents are used.

Patent Document 1:

Accordingly, the present inventor has developed a composition for preventing or treating aging and arthritis containing a herbal composition as an active ingredient.

Accordingly, the present invention provides a composition for preventing or treating aging and arthritis.

The present invention relates to a composition for preventing or treating aging and arthritis comprising honey, dermis, dermis, genesis, gill root, licorice root, ginseng root, peppermint, gardenia and chest chest cartilage extract powder.

The composition according to the present invention has an excellent effect in inhibiting hyaluronidase activity and skin wrinkles and increasing skin elasticity, bone mineral density and elasticity.

The present invention relates to a composition for preventing or treating aging and arthritis comprising honey, dermis, dermis, genesis, gill root, licorice root, ginseng root, peppermint, gardenia and chest chest cartilage extract powder.

Wherein the composition comprises 1 to 10 parts by weight of the composition, 1 to 20 parts by weight of honey, 1 to 10 parts by weight of the dermis, 5 to 20 parts by weight of the dandelion, 5 to 20 parts by weight of the dandelion, 1 to 10 parts by weight of licorice, 5 to 20 parts by weight of gut root, 5 to 20 parts by weight of gum roots, 1 to 10 parts by weight of peppermint, 1 to 10 parts by weight of gardenia, and 5 to 40 parts by weight of chest chest cartilage extract powder .

The composition may be in the form of powders, tablets, capsules, granules, pills or solutions.

When the composition is a granule or a pill, the content of honey is preferably 1 to 20 parts by weight based on 100 parts by weight of the total composition. When the composition is added in an amount of less than 1 part by weight, it is difficult to form a composition. , The viscosity is greatly increased, which makes it difficult to dry and the finished products are stuck together, making it difficult to maintain their shape.

The composition may inhibit hyaluronidase activity. Hyaluronic acid is a substance contained in many cartilage tissues in vivo, plays a major role in improving the elasticity of cartilage tissue, has high moisture content, and has excellent viscoelasticity. In general, accelerated degradation of hyaluronic acid is observed in osteoarthritis patients. There are reports that hyaluronidase and the like increase the cell death of articular cartilage cells and decrease the metabolism of cartilage cells to promote degenerative changes of cartilage tissue. Therefore, inhibiting hyaluronidase, a degrading enzyme of hyaluronic acid, has an effect of treating osteoarthritis.

The composition may be a pharmaceutical composition or a food composition.

Hereinafter, the natural products used in the present invention will be briefly described.

Radix Bupleuri (Latin name: Bupleurum falcatum L., Umbelliferae) is the first Chinese medicinal herb in the Divine Materia Medica. It has symptoms of repeated colds and fever, chest discomfort, Headache, dizziness, schizophrenia, withdrawal, menstrual relief, uterine drainage, and hernia. In the case of bupleurumol, oleic acid, linoleic acid, palmitic acid, saikosaponin A, B, C, saikogenin F, E , G), and the like.

Honey has been known to have the effects of restoring fatigue, preventing and treating anemia, supplying diabetic patients, prevention and treatment of liver diseases, relieving hangover, natural nutritional supplement, beauty effect, infant growth promotion and sterilization effect . Fructose, the main component of honey, is a natural sweetener and is used as a food ingredient.

The dandruff refers to the peel of citrus unshiu and its plants belonging to the genus Rutaceae distributed throughout Korea and its components are known as a large amount of flavonoids and vitamins. , Dryness, dryness, and so on.

Angelica acutiloba KITAGAWA belongs to the genus Umbelliferae distributed throughout Korea and refers to the roots of herbivorous plants such as ligustilide, butylidenephthalide, Components such as Cnidilide, Isocnilide, sesquiterpene and the like are known, and a therapeutic action for dysmenorrhea, abdominal pain and the like are known.

It is effective for sweating, diarrhea, analgesic and dryness, etc. It is effective for gastroenteritis and cold, especially for suppressing and stimulating central nervous excitement, and has an excellent effect on obesity. Atractylone, Atractylol and vitamins A and D are the main pharmacological ingredients.

Pseudoptera (Porphyra) is a relative plant belonging to the same genus. This medicine has a peculiar smell, and the taste is spicy and the quality is warm. It is used for chest leaks caused by mental anxiety, insomnia, easily frightening symptoms, nervous breakdown, myocarditis, postpartum heart disease, heart failure, altitude sickness, menstrual irregularity, stomach cramps, joint points, bruises. Pharmacological actions such as sedation, antipyretic action, hypotension, Gram-positive bacterial inhibitory action, and diuretic action have been reported.

Licorice (Glycyrrhiza uralensis FISCH.) Is a perennial herb of soybeans. Licorice is harmonized with all medicines in herbal medicine and increases the efficacy. In addition, it alleviates various pain and urgency symptoms by relieving pain medication. And it has its own weakness, such as neutralizing action of poison. It contains all of triterpene saponin and glycyrrhizin in roots and rhizomes of plant. It has all the detoxification effects against bacterial toxins as well as drug poisoning, food poisoning, metabolism of body metabolism, And thyroid gland hormone levels.

It is a bark of roots and has no smell. Changgeun sweat, fever fever, high fever, cure headache and stop the thirst. It is used for digestion, headache, anemia, dysentery, abdominal pain, dryness, cold, vomiting and bleeding of wife. The fruit of the 칡 is called the gag, and it is effective in the diarrhea. The 칡 갈 갈 갈 갈 갈 갈 갈 갈...............................

Byeongryeong lowering effect of blood sugar, prevention of gastric ulceration, dryness, diuretic, sedation, heart palpitations, such as a wide range of findings revealed that the ingredients of the glucose polysaccharide pachyman (pachyman) and diuretic sedation, It contains pachymicacid, polyporenic acid and ergosterol as a steroid. In addition to methyltumulosate, various components such as albumin are contained in the Korean gyojil.

Mint is a perennial herbaceous color belonging to the family Lamiaceae. It is effective for symptoms of fever, headache and sweating caused by colds of the ears. It causes redness of eyes, sore throat, tonsillitis, early itching skin, liver disease, abdominal pain, diarrhea and vomiting The effect of pharmacological action on fever, inflammation, dryness, bile secretion promotion and gastrointestinal smooth muscle suppression, increased mucus secretion of the respiratory tract, central nervous excitement, and uterine contractions have been reported.

Gardeniae Fructus has long been used as a food coloring agent in the gardenia (Gardenia jasmoides Ellis) belonging to the Rubiaceae family or as a fruit of other plants. In one room, inflammation, diuresis, hemostasis. It is used as antipyretic, sedative, antimicrobial, bile secretion, hepatitis, jaundice, and hematemesis. The pharmacological action of gardenia has been reported to promote the secretion of bile and to protect against the chemical properties of soy sauce.

Chicken Sternum Cartilage (Chicken Sternum Cartilage) is a type of collagen-type two-type structure composed of protein, chondroitin sulfate, moisture, phosphorus and ash. Collagen is an important protein that constitutes the living body. It is widely distributed in the skin and articular cartilage, and is widely distributed throughout the ligaments, bones, and blood vessels. It is classified into type 1, type 2, type 3 to type 20 depending on the distribution and properties in vivo. Among them, the major component of joints is collagen of type 2, except for water. This ingredient acts as a lubricant for the cartilage, and it has been reported that it attracts moisture in the tissue to help soften the movement of joints, thereby alleviating the pain of osteoarthritis and rheumatism, and slowing degeneration of cartilage. In addition, there is a record of prescription of "Huangzhong" rib (黄 雌 鷄 rib bone) on "Huancho" which is an old Chinese medicine book.

In addition, the present invention provides a composition for preventing and treating arthritis, which is produced by the above-described method.

The composition of the present invention is a pharmaceutical composition containing a herbal composition consisting of chickpea, honey, dermis, Angelica keiskei, kujitsu root, licorice root, ginger root, mint, gardenia, and chest chest cartilage extract powder.

The pharmaceutical compositions for the prevention or treatment of aging and arthritis of the present invention may further contain commonly used excipients, disintegrants, sweeteners, lubricants, flavors and the like, and may be formulated into tablets, capsules, powders, Granules, suspensions, emulsions, syrups, and other liquid preparations. Specifically, the pharmaceutical compositions of the present invention may be formulated for oral administration, for example, tablets, troches, lozenges, aqueous or oily suspensions, pharmaceutical powders or granules, emulsions, hard or soft capsules, It is formulated into elixirs.

Binders such as lactose, saccharose, sorbitol, mannitol, starch, amylopectin, cellulose or gelatin for formulation in tablets, capsules and the like; Excipients such as dicalcium phosphate; Disintegrating agents such as corn starch or sweet potato starch; Lubricating oil such as magnesium stearate, calcium stearate, sodium stearyl fumarate or polyethylene glycol wax. In the case of a capsule formulation, in addition to the above-mentioned substances, a liquid carrier such as fatty oil is contained.

In addition, the pharmaceutical composition of the present invention can be administered parenterally, and parenteral administration is by subcutaneous injection, intravenous injection, intramuscular injection, or chest injection. In order to formulate the formulation for parenteral administration, the herbal composition is mixed with a stabilizer or a buffer in water to prepare a solution or suspension, which is then formulated into a unit dosage form of ampoule or vial.

The dosage of the active ingredient according to the present invention is appropriately selected depending on the degree of absorption, inactivation rate and excretion rate of the active ingredient in the body, age, sex and condition of the patient, and severity of the disease to be treated.

In the present invention, arthritis refers to all kinds of arthritis, including arthritic arthritis, rheumatoid arthritis, degenerative arthritis, gouty arthritis, bacterial arthritis and purulent arthritis.

The composition for preventing or treating arthritis and arthritis of the present invention can be prepared from foods, especially functional food compositions. The functional food composition of the present invention includes components that are ordinarily added during the manufacture of food, and includes, for example, proteins, carbohydrates, fats, nutrients, and seasonings. For example, when it is made of a drink, a flavoring agent or a natural carbohydrate may be added as an additional ingredient in addition to the extract of zucchini as an active ingredient. For example, natural carbohydrates include monosaccharides (e.g., glucose, fructose, etc.); Disaccharides (e.g., maltose, sucrose, etc.); oligosaccharide; Polysaccharides (e.g., dextrin, cyclodextrin and the like); And sugar alcohols (e.g., xylitol, sorbitol, erythritol, etc.). Natural flavoring agents (e.g., tau martin, stevia extract, etc.) and synthetic flavoring agents (e.g., saccharin, aspartame, etc.) may be used as flavorings. Considering the easy accessibility to food, the food of the present invention is very useful for the treatment or prevention of aging and arthritis.

Hereinafter, preferred embodiments of the present invention will be described. However, the following examples are given to aid understanding of the present invention, and the present invention is not limited to the following examples.

Examples 1 to 5: Preparation of a herbal composition for prevention or treatment of aging and arthritis according to the present invention [

According to the ratio shown in Table 1, 1 to 10% by weight of Seal, 1 to 10% by weight of Dermis, 5 to 20% by weight of Angelica keiskei, 5 to 20% 5 to 20% by weight of Phellinus linteus, 5 to 20% by weight of Phellinus linteus, 1 to 10% by weight of peppermint and 1 to 10% by weight of Gardenia were crushed by a pulverizer and homogeneously mixed. Then, 1 to 20 wt% of honey and 5 to 40 wt% of chest chest cartilage extract powder were homogeneously mixed.

The resulting mixture was then aged at a low temperature of about 4 DEG C for 24 hours. After the ripening, the size of the mung bean sprouts was naturally dried.

<Experimental Example 1: Measurement of hyaluronidase activity inhibition degree>

In order to examine the inhibition of hyaluronidase activity of the composition, activity was measured using Morgan-Elson Assay. Hyaluronic acid dissolved in phosphate buffer solution and the extract mixture prepared in the above example dissolved in DMSO were added to each well and cultured at 37 ° C for 20 minutes. After incubation, the compound 48/80 solution was added and secondary cultured. After hyaluronic acid was added and cultured for the third time, K3BO3 was added and the reaction was stopped by heating in hot water. After centrifugation, N-acetylglucosamine, which is produced by degradation of hyaluronidic acid by hyaluronidase, was quantified by colorimetric determination of 585 with DMAB (p-dimethyaminobenzaldehyde) to confirm its activity. Celecoxib (Pfizer) was used as a positive control. The results are shown in Table 2.

As shown in Table 2, in Example 3 of the mixture of the present invention, the activity inhibition degree of hyaluronidase was 64.2 ± 0.53%, which was similar to or slightly higher than that of positive control celecoxib activity inhibition, And the inhibition effect was high.

<Experimental Example 2: skin elasticity test>

Skin elasticity effects were measured with the mixtures prepared according to Examples 1 and 3.

The measurement method was as follows: 10 healthy women without skin disease were divided into left and right crows feet at 25 ° C, 50% relative humidity and room temperature and humidity conditions. 1 in the right side, and 4 weeks in the right side, and 8 grams of the mung bean egg-size ring was divided into small portions and packed for 30 minutes before breakfast. The elasticity of the skin was measured by Cutometer MPA580 (Courage and Khazaka, Kiln, Germany).

The R2 value in the measuring equipment means the gross elasticity of the skin due to the ability to re-deform the skin. In Example 1, the R2 value was increased by 13.6% in the measurement result, and in Example 3, the R2 value was increased by 18.9% in the measurement result, and the skin elasticity was increased.

Experimental Example 3: Skin wrinkle test [

The wrinkle effect of the skin was measured with the mixture prepared in Example 3.

The subjects were 10 healthy women without skin disease at 25 ℃, 50% relative humidity and room temperature and humidity condition. The subjects were divided into left and right eyes at the crows feet, The rats were divided into two groups of 8 grams of green bean sprout sized for 6 weeks and divided into two groups and fed 30 minutes before breakfast. And then analyzed for skin wrinkles by performing image analysis on replica skin using Visiometer (Skin-Visiometer SV 600, Courage & Khazaka, Germany).

R3 value means Average Roughness. In other words, it is a direct parameter related to the depth of wrinkles, so it was observed that there was a difference in the wrinkle-improving effect because the difference between the R3 value of 6 weeks (0.184) and 0 week (0.236) was decreased by -0.052.

<Experimental Example 4: Animal effect test>

The following experiments were conducted to confirm the bone density and the elasticity effect of the bone bioassay.

Ten rats weighing 240-260 g were randomly divided into two groups of five animals as experimental animals. A control group (ovariectomized rats, protein containing 4000 mg / kg calcium base protein) and test group (ovariectomized rats, feed mixture of Example 3 10 g / kg). After one week of adaptation period, 2% sodium pentobarbitol solution was anesthetized by intraperitoneal injection and the ovaries were excised. Two days after the operation, the vaginal examination was performed, and after 5 days, the oestrus cycle was confirmed and the success was confirmed. (1 g / kg of protein containing 4000 mg / kg calcium) in a negative control group for 8 weeks after one week of operation, and 10 g / kg of the mixture of Example 3 in the test group. After 8 weeks of testing, the mice were dissected and the surrounding thigh tissue was removed to fill the right thighs and tibia. And kept at minus 70 degrees. Tibia was stored in gauze impregnated with physiological saline solution. After 1 day, bone mineral density and elasticity were tested.

The bone mineral density of the femur was increased by 3.7% as compared with the control group (1.209 ± 0.032) (1.254 ± 0.033), and the elasticity test showed that the control group (309.1 ± 65.9) % Elasticity was increased and the effect was shown on the joints.

Claims (7)

delete 1 to 10 parts by weight of starch, 1 to 20 parts by weight of honey, 1 to 10 parts by weight of dermis, 5 to 20 parts by weight of angelica ginseng, 5 to 20 parts by weight of guinea pig, 1 to 10 parts by weight of ginseng root, Characterized in that the agar is 1 to 10 parts by weight of licorice, 5 to 20 parts by weight of germanium, 5 to 20 parts by weight of germanium, 1 to 10 parts by weight of peppermint, 1 to 10 parts by weight of gardenia, and 5 to 40 parts by weight of chick chest cartilage extract powder. And compositions for the prophylaxis or treatment of arthritis. The composition for preventing or treating aging and arthritis according to claim 2, wherein the composition inhibits hyaluronidase activity. 3. The composition of claim 2, wherein the composition is a pharmaceutical composition. 3. The composition of claim 2, wherein the composition is a food composition. The composition for preventing or treating aging and arthritis according to claim 2, wherein the composition is in the form of powders, tablets, granules, pills, capsules or liquid preparations. [3] The composition for preventing or treating aging and arthritis according to claim 2, wherein the seeds, dermis, Angelica keiskei keishi, gill root, licorice root, gypsum root, peppermint, and gardenia are dried and pulverized, respectively.