KR101555234B1 - The method for manufacuting momordica charantia, and the inclusion compound made by the method - Google Patents

Abstract

The present invention relates to a method for manufacturing yeast inclusion compound which is prepared by preparing yeast in the form of powder or concentrate and then encapsulating the yeast with cyclodextrin to enhance the flavor of Yeoju, To a quinching inclusion compound prepared by the above method.

Description

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a method for producing a chelating inclusion compound having reduced bitter taste of Yeoju and a method for producing the inclusion inclusion compound,

The present invention relates to a method for manufacturing a yeast inclusion compound which is prepared by preparing a yeast in the form of a powder or a concentrate and then incorporating the beta-cyclodextrin into the yeast to reduce the bitter taste unique to Yeoju and enhance the flavor of Yeoju, And a quinching inclusion compound prepared by the above method.

Momordica Charantia is a medicinal plant belonging to the genus Cucurbitaceae. Its components are beta-sitosterol-beta-D-glucoside and 5,25-stigmatadien-3beta-D- Glucoside, 5-hydroxytryptamine and various amino acids such as glutamic acid, alanine, beta-alanine, and 5-hydroxytryptamine. beta-alanine, phenylalanine, proline, alpha-aminobutyric acid, citrulline, galacturonic acid, vitamin C, It has been reported that beta-carotin, potassium, iron, charantin, plant insulin, CLA (conjugated linoleic acid) and pectin (pectin) ) And the like.

 Yeoju has the effects of reducing thirst, improving appetite, improving skin health, improving immunity, anticancer, antipyretic, antioxidant and bronchial strengthening, obesity, cardiovascular disease, diabetes, skin disease, night blindness, parasite, rheumatism, Gout, and weakness of the body.

The efficacy for major diseases will be briefly described according to the components of the females listed above.

CLA, a major component of Yeoju, is an anti-mutagenic substance discovered by Dr. Panza's team at the University of Wisconsin Madison in 1987. Since it has been inadvertently separated during the search for anticancer activity in fined ground beef, Is an intermediate metabolite synthesized by ruminant microorganisms from inoleic acid, which is present in trace amounts in natural foods. Antioxidant, anti-cancer, anti-arteriosclerosis, immunity enhancement, and body fat reduction have already been proven.

CLA increases hormone sensitive loss and activity of camitne palmitoyl transferase in rats to reduce body fat and increase lean body mass (muscular). In addition, CLA has been shown to reduce body weight by an average of 2.5kg and body fat by 15 ~ 20% three months after ingesting 1 ~ 3g daily.

Among the efficacy of Yeoju, the widely known diabetes-improving effect is due to plant insulin, carantine and CLA contained in Yeoju. Plant insulin, like the insulin secreted in the body, promotes the burning of glucose in the liver and prevents the recombination of glucose in the body, thereby lowering the blood glucose level of diabetic patients. Carantine acts as a pancreas that secretes insulin Promote.

 Several studies have shown that Yeoju has the effect of improving diabetes. For example, researchers at the Tokyo Medical University in Japan have found that mice with high blood sugar levels have lowered blood glucose levels in mice within a week after administering a certain concentration of lute juice extract.

Studies have shown that CLA activates PPARy in adipose tissue to restore glucose tolerance to normal and increases insulin levels. Yeoju is known to have no side effects, unlike the hypoglycemic agents used in the medical field.

In addition, the improvement effect of Yeoju's cardiovascular diseases (retinopathy, nephropathy, myocardial infarction and stroke) is due to the antioxidant components such as vitamin C and beta-carotene, which are contained in the flesh of Yeoju large amounts. Vitamin C and beta- And increases the production of prostaglandins in vascular endothelial cells that play a role in vascular relaxation and platelet aggregation inhibition. Reduces LDL cholesterol and eliminates peroxides and reactive oxygen species (ROS).

It is known that Vitamin C in Yeoju is hardly destroyed when heated, and Vitamin C in Yeoju is 120mg of Yeoju 100g. This is much more than 80mg of strawberry, 40mg of cabbage and 90mg of lemon.

In Gangjin County, Yeoju was selected as one of the export items because it has high marketability.

However, in spite of the efficacy of Yeoju, Yeoju's bitter taste has not been appreciated in favor of young people and foreigners, resulting in high added value of Yeoju products and obstacles to advance into overseas market.

Therefore, it is necessary to search for a technique that can eliminate the bitterness of Yeoju without changing the ingredients of Yeoju.

The conventional method of removing bitter taste of Yeoju is to salt Yeoju or to mix foods with strong fragrance such as lime juice and curry powder.

However, the above method is to mix water, salt, or other additives in the yeast, and the ingredients and efficacy of Yeast can be changed when the method is performed.

Accordingly, the present applicant has discovered a method for producing an inclusion compound by reacting cyclodextrin with Yeast, as a method for maintaining the efficacy while eliminating the bitter taste of Yeoju. The cyclodextrin is as follows.

Cyclodextrin is a cyclic oligosaccharide produced by enzymatic reaction with starch as a raw material. The inside of the cyclodextrin molecule is composed of hydrogen and oxygen, and the outside is composed of a hydroxyl group. It is a white crystal or crystalline powder with no odor and a slight sweet taste. It has heat resistance against various acids and alkali, and is also resistant to heating and humidity.

The greatest characteristic of cyclodextrin is its inclusion. The inclusion is a phenomenon in which, when two molecules are paired, a relatively larger molecule spreads in a tubular, layered or net shape, and a smaller molecule is stuck into the gap to join the two molecules. Since the molecular structure of cyclodextrin is ring-shaped, it can cover other molecules. The inside of the cyclodextrin molecule is hydrophobic, composed of hydrogen and oxygen, and the outside is hydrophilic.

In other words, there is no factor that causes changes in the components and efficacy of Bacchaeus and Lycaeus by using cyclodextrin, so that the bitter taste can be eliminated without changing the components and efficacy of Bacillus.

Meanwhile, Korean Laid-open Patent Publication No. 10-2013-0092897 discloses a health functional ring manufactured by using a lacquer and a method for producing the same, and Korean Patent Laid- 2011-0086903 discloses a sikhye and a manufacturing method using yeast prepared by using yeast.

Korean Patent Registration No. 10-1300386 discloses a method for manufacturing a parking lot using berries, leaves, stems and roots.

The above prior arts are consistent with the present invention in that they utilize the yeast as a material, but since the drying, roasting, or addition of the yeast in the bitter taste removal process is performed, the composition and efficacy of the yeast are changed .

Korean Patent Publication No. 10-2013-0092897 (2013. 08. 21) Korean Patent Publication No. 10-2011-0086903 (2011.08.02) Korean Registered Patent No. 10-1300386 (Feb.

Lee Hee-joo and 6 others. Fruit characteristics and Charantin content of Yeoju by genetic data. Journal of Biomedical Engineering. Vol.21, no.4, pp.379-384, 2012

It is an object of the present invention to provide an inclusion compound preparation method in which a yeast is prepared in the form of a powder or a concentrate and encapsulated with cyclodextrin to thereby reduce the bitter taste unique to Yeoju to enhance the flavor of Yeoju, To provide inclusion compounds.

In order to solve the above technical problems, there is provided a method for producing a solution, comprising the steps of: dissolving a predetermined amount of a yeast concentrate or a butterfly powder and a predetermined amount of cyclodextrin in a predetermined amount of 0.1 N acetic acid to form a solution; Dropwise adding 1.0 N sodium hydroxide (NaOH) so that the solution has a pH of 10.0 to 12.0; Dropwise addition of 0.1 N hydrochloric acid (HCl) so that the solution has a pH of 6.0 to 8.0; And filtering the solution, followed by freeze-drying or spray-drying the solution.

Adding a certain amount of the yeast concentrate or a predetermined amount of the frying powder to a predetermined amount of a 40 to 60% ethanol solution, stirring and dissolving the solution, and adding a predetermined amount of cyclodextrin to the solution to produce a solution; Heating the solution to 70 to 90 캜, reacting the solution for 2 to 5 hours, stopping the heating, and stirring at room temperature for 24 hours; And then freeze-drying or spray-drying the solution after filtration under reduced pressure.

Adding a predetermined amount of cyclodextrin, a concentrated yeast concentrate, or a predetermined amount of fumarate powder to a predetermined amount of distilled water and stirring the mixture at 30 to 40 ° C to dissolve the solution; And freeze-drying or spray-drying the solution.

Also provided is a quinching inclusion compound prepared by any one of the above methods.

The present invention also provides a composition comprising an effective inclusion compound of the present invention prepared by the method described in any one of the above methods.

The health functional food using the composition according to the present invention may be provided in various forms such as a functional beverage, a health supplement, tea, confectionery, and the like.

To be used as a food composition, the composition according to the present invention may contain ingredients that are conventionally added in the manufacture of food. For example, proteins, fats, nutrients, flavoring agents and flavoring agents. As a flavoring agent, a natural flavoring agent [tau martin, stevia extract (rebaudioside A, glycyrrhizin, etc.)) and a synthetic flavoring agent (saccharin, aspartame, etc.) can be used.

Formulations such as pills, granules, beverages, tablets, capsules and the like may be prepared using the composition according to the present invention, in which case additives may be added to prepare each formulation.

In order to use the present invention as a pharmaceutical composition, it may be prepared by a known method in the pharmaceutical field, and may be mixed with the carrier itself, or a pharmaceutically acceptable carrier, excipient, diluent or the like to prepare a powder, granule, Or injections, and the like. They may also be administered orally or parenterally.

May be formulated in the form of powders, granules, tablets, capsules, suspensions, emulsions, oral preparations such as syrups and aerosols, external preparations, suppositories and sterilized injection solutions according to a conventional method, Carriers, excipients and diluents which may be included include lactose, dextrose, sucrose, sorbitol, mannitol, starch, acacia rubber, alginate, gelatin, calcium phosphate, calcium silicate, cellulose, methylcellulose, microcrystalline cellulose, poly Vinyl pyrrolidone, water, methylhydroxy, hydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate, and mineral oil.

In the case of formulation, a diluent or excipient such as a filler, an extender, a binder, a wetting agent, a disintegrant, or a surfactant is usually used.

Solid formulations for oral administration include tablets, pills, powders, granules, capsules and the like, which may contain at least one excipient such as starch, calcium carbonate, sucrose, ), Lactose, gelatin and the like. In addition to simple excipients, lubricants such as magnesium stearate talc are also used.

Examples of the liquid preparation for oral use include suspensions, solutions, emulsions, and syrups. In addition to water and liquid paraffin, simple diluents commonly used, various excipients such as wetting agents, sweeteners, fragrances, preservatives and the like may be included .

Formulations for parenteral administration include sterile aqueous solutions, non-aqueous solutions, suspensions, emulsion-freeze-dried preparations, and suppositories. Examples of the suspending agent include propylene glycol, polyethylene glycol, vegetable oil such as olive oil, injectable ester such as ethyl oleate, and the like.

As the base of the suppository, witepsol, macrogol, tween 61, cacao paper, laurin, glycerogelatin and the like can be used.

The effective dose of the composition according to the present invention can be appropriately selected depending on the degree of absorption of the active ingredient in the body, the rate of water activation and excretion, the age, sex and condition of the patient, severity of the disease to be treated,

The composition according to the invention can be used in feed for livestock and in each case can be prepared using additives or additives which are acceptable in livestock feed.

In order to use the composition according to the present invention as an active ingredient of a cosmetic composition, the ingredients contained in the cosmetic composition include ingredients commonly used in cosmetic compositions, in addition to the extract of Yeast extract. Such as antioxidants, stabilizers, solubilizers, vitamins, customary adjuvants such as pigments and flavoring agents, and carriers.

The cosmetic composition according to the present invention can be prepared into any formulation conventionally produced in the art. For example, they may be formulated as solutions, suspensions, emulsions, pastes, gels, creams, lotions, powders, soaps, surfactant-containing cleansing, oils, powder foundations, emulsion foundations, wax foundations and sprays, But is not limited thereto.

More specifically, it can be manufactured in the form of a soft lotion, a nutritional lotion, a nutritional cream, a massage cream, an essence, an eye cream, a cleansing cream, a cleansing foam, a cleansing water, a pack, a spray or a powder.

When the formulation of the present invention is a solution or an emulsion, a solvent, a dissolving agent or an emulsifying agent is used as a carrier component, and examples thereof include water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, , 3-butyl glycol oil, glycerol aliphatic ester, polyethylene glycol or sorbitan fatty acid esters.

When the formulation of the present invention is a suspension, a carrier such as water, a liquid diluent such as ethanol or propylene glycol, a suspension such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, Crystalline cellulose, aluminum metahydroxide, bentonite, agar or tracant, etc. may be used.

When the formulation of the present invention is an interfacial active agent-containing cleansing, the carrier component may include aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyltaurate, sarcosinate, fatty acid amide Ether sulfates, alkylamidobetaines, aliphatic alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, lanolin derivatives, or ethoxylated glycerol fatty acid esters.

When the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component. In the case of a spray, in particular, / Propane or dimethyl ether.

When the formulation of the present invention is a paste, cream or gel, an animal oil, vegetable oil, wax, paraffin, starch, tracer, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide may be used as the carrier component .

The cosmetic composition containing the composition according to the present invention as an active ingredient comprises a composition selected from the group consisting of water-soluble vitamins, oiliness vitamins, sphingolipids, polymeric polysaccharides, and polymer peptides.

Examples of the water-soluble vitamin include vitamin B1, vitamin B2, vitamin B6, pyridoxine, pyridoxine hydrochloride, vitamin B12, pantothenic acid, nicotinic acid, nicotinic acid amide, folic acid, vitamin C and vitamin H And their salts (thiamine hydrochloride, sodium ascorbate, etc.) or derivatives (sodium ascorbic acid-2-phosphate, magnesium ascorbate-2-phosphate etc.) can also be added to water-soluble vitamins . The water-soluble vitamin can be obtained by a conventional method such as a microorganism conversion method, a purification method from a culture of a microorganism, an enzymatic method, or a chemical synthesis method.

Examples of useful vitamins include vitamin A, carotene, vitamin D2, vitamin D3, vitamin E (DL-alpha tocopherol, D-alpha tocopherol, D-alpha tocopherol and the like) , Derivatives thereof (such as palmitic acid ascorbin, stearic acid ascorbic acid, dipalmitic acid ascorbin, acetic acid DL-alpha tocopherol, nicotinic acid DL-alpha tocopherol vitamin E. DL-pantothenyl alcohol, D-pantothenyl alcohol, Ether, etc.) are also included in the usable vitamins used in the present invention. Usability Vitamins can be obtained by a conventional method such as a microorganism conversion method, a purification method from a culture of a microorganism, an enzyme or a chemical synthesis method.

Sphingo lipids may be any as long as they can be incorporated into cosmetics, and preferable examples include ceramides, phytosphingosine and sphingoglycolipids. Sphingoid lipids can be purified from ordinary mammals, fish, shellfish, yeast or plants by conventional methods or can be obtained by chemical synthesis.

The polymer polysaccharide may be any compound as long as it can be compounded in cosmetics, and examples thereof include hydroxyethyl cellulose, xanthan gum, sodium hyaluronate, chondroitin sulfate or a salt thereof (sodium salt, etc.). For example, chondroitin sulfate or a salt thereof can be usually purified from mammals or fishes.

The polymeric peptide may be any compound as long as it can be compounded in the cosmetic, and preferably collagen, hydrolyzed collagen, gelatin, elastin, hydrolyzed elastin, keratin and the like. The polymeric peptide can be obtained by a conventional method such as purification from a culture broth of a microorganism, an enzymatic method, or a chemical synthesis method, or it can be purified from natural products such as ducks such as pigs and cows and silk fiber of silkworms.

The cosmetic of the present invention may be blended with other essential ingredients, if necessary, in combination with the essential ingredients. Examples of the compounding ingredients that may be added include organic solvents such as a preservative component, a moisturizer, an emollient, a surfactant, an organic and inorganic pigment, an organic powder, an ultraviolet absorbent, a preservative, a bactericide, an antioxidant, a plant extract, a pH adjuster, A blood circulation promoter, a cold agent, a restriction agent, and purified water.

The components to be added in addition to the above are not limited to the above, and all of the above components can be blended within the range not to impair the objects and effects of the present invention.

The method for manufacturing a female inclusion compound according to the present invention is a method for producing a female inclusion compound which has an improving effect on cardiovascular diseases, diabetes, skin diseases, night blindness, parasites, rheumatism, gout and weakness of the body, It has the effect of reducing the bitter taste of Yeoju and enhancing the preference degree by covering with dextrin.

The terms and words used in the present specification and claims should not be construed as limited to ordinary or dictionary terms and the inventor may properly define the concept of the term in order to best describe its invention It should be construed as meaning and concept consistent with the technical idea of the present invention.

Therefore, the embodiments described in the present specification, the reference examples, and the drawings are merely the most preferred examples of the present invention, and not all of the technical ideas of the present invention are described. Therefore, It should be understood that various equivalents and modifications may be present.

Manufacturing example  1. Yeoju Inclusion compound  Preparation: Acetic acid dissolution and pH  control

1. Manufacture of Yeoju concentrate

Wash out the leaves and wash them three times. Extracted Yeast Extract is prepared in a concentration of 60-70 brix at 90 ℃ or lower and stored in the refrigerator.

2. Manufacture of Yeoju Powder

After removing the moisture of the yeast, freeze-dried, it is finely pulverized to 90 ~ 110μm.

3. Yeoju dissolved in acetic acid Inclusion compound  Produce

1, or a certain amount of the flour powder of 2 and a certain amount of cyclodextrin are dissolved in a predetermined amount of 0.1 N acetic acid.

1.0 N sodium hydroxide (NaOH) is gradually added dropwise thereto so that the pH of the solution becomes 10.0-12.0, and then 0.1N hydrochloric acid (HCl) is slowly added dropwise thereto so that the pH of the solution becomes 6.0-8.0.

The solution is filtered and then lyophilized or spray-dried to prepare a sheath inclusion compound.

The weight ratio of the baking powder or the baking powder to the cyclodextrin is not limited, and the weight ratio is preferably 1: 1 to 1: 3, but is not limited thereto.

Also here, the cyclodextrin can be selected from?,? And? -Cyclodextrins.

Manufacturing example  2. Yeoju Inclusion compound  Manufacturing: Ethanol dissolution

1. Manufacture of Yeoju concentrate

The same procedures as in Production Example 1 were carried out.

2. Manufacture of Yeoju Powder

The same procedures as in Production Example 1 were carried out.

3. Yeoju dissolved in ethanol Inclusion compound  Produce

Add a certain amount of yeast concentrate or lump of powder into a certain amount of 40 ~ 60% ethanol solution, dissolve by stirring, and add a certain amount of cyclodextrin. The mixture is heated to 70-90 ° C and reacted for 2-5 hours, then the heating is stopped and stirring is continued at room temperature for 24 hours. After stopping the stirring, the reaction solution is filtered under reduced pressure and freeze-dried or spray-dried to prepare a kneading inclusion compound.

The weight ratio of the baking powder or the baking powder to the cyclodextrin is not limited, and the weight ratio is preferably 1: 1 to 1: 3, but is not limited thereto.

Also here, the cyclodextrin can be selected from?,? And? -Cyclodextrins.

Manufacturing example  3. Yeoju Inclusion compound  Manufacturing: Water dissolving

1. Manufacture of Yeoju concentrate

Was prepared in the same manner as in Production Example 1.

2. Manufacture of Yeoju Powder

Was prepared in the same manner as in Production Example 1.

3. Yeoju dissolved in water Inclusion compound  Produce

To a predetermined amount of distilled water, a predetermined amount of cyclodextrin, a concentrated yeast concentrate, or a predetermined amount of yeast powder is added, and the mixture is stirred and dissolved at 35 to 40 ° C, followed by lyophilization or spray drying to prepare a quercetin inclusion compound.

The weight ratio of the baking powder or the baking powder to the cyclodextrin is not limited, and the weight ratio is preferably 1: 3, but is not limited thereto.

Also here, the cyclodextrin can be selected from?,? And? -Cyclodextrins.

Reference Example  1. Yeoju Inclusion compound  Sensory evaluation

1. Experimental process

To remove the bitter taste of Yeoju, the sensory evaluation of Yeoju powder prepared by different manufacturing process was performed on 20 researchers in the institute.

Five items of color, flavor, bitterness, taste, and overall acceptability of the powder were evaluated. Five points were used to evaluate the bitterness, And the strength was measured to be weakened.

The kneading inclusion compound prepared by the processes of Preparation Examples 1 to 3 was used as an experimental material.

Production Example 1 was carried out as follows.
A certain amount of the Yeoju concentrate or the lump of powder and a certain amount of cyclodextrin were dissolved in a predetermined amount of 0.1 N acetic acid.
1.0 N sodium hydroxide (NaOH) was slowly added dropwise thereto so that the pH of the solution became 10.0-12.0, and 0.1N hydrochloric acid (HCl) was slowly added dropwise thereto so that the pH of the solution became 6.0-8.0.
The solution was filtered and then lyophilized or spray-dried to prepare a sheath inclusion compound.

Production Example 2 was carried out as follows.
The concentrate of Yeoju or a certain amount of Yeoju powder was added to a certain amount of 40 ~ 60% ethanol solution and dissolved by stirring, and then a certain amount of cyclodextrin was injected.
The mixture was heated to 70-90 ° C and allowed to react for 2-5 hours, then the heating was stopped and stirring was continued at room temperature for 24 hours.
After the stirring was stopped, the solution was filtered under reduced pressure, and freeze-dried or spray-dried to prepare a kneading inclusion compound.

Production Example 3 was carried out by the following procedure.
A predetermined amount of cyclodextrin, a concentrated yeast concentrate, or a predetermined amount of powdery mallow powder was added to a predetermined amount of distilled water, followed by stirring at 35 to 40 ° C.
After stopping the stirring, the solution was freeze-dried or spray-dried to prepare a kneading inclusion compound.

2. Experimental results

Table 1 below is a table showing the sensory evaluation of samples of pasty powder in which the bitter taste was removed by covering with cyclodextrin.

characteristic
(Characteristics)
sample The control (control) Production Method 1 /
Yoojoo inclusion compound group 1
(pH adjustment) Production Method 2 /
Yeoju inclusion compound 2 group
(Ethanol addition) Production Method 3 /
Group 3
(Water addition) 24h 24h 24h color
(Color) 3.01 ± 0.67 2.23 ± 1.07 2.81 0.75 2.99 ± 1.42 bitter
(Bitterness) 1.32 0.66 2.72 0.74 2.76 ± 1.20 3.07 ± 0.92 incense
(Flavor) 2.25 ± 1.32 2.08 ± 1.71 3.15 ± 1.09 3.11 ± 1.10 flavor
(Taste) 2.66 ± 1.70 2.13 + 1.31 3.02 ± 0.92 3.50 1.81 Overall likelihood
(Overall
acceptance) 2.80 ± 1.22 3.00 0.90 3.10 ± 0.82 3.23 ± 0.71

The preference of the control group without any treatment was the highest at 3.01 ± 0.67 and the preference of the control group at 1 was lowest at 2.23 ± 1.07.

In the bitter taste category, the preference of female group 3 of clathrate compound was 3.07 ± 0.92 And the preference of the control group was 1.32 ± 0.66 The lowest.

In the fragrance items, the preference of the female clathrate compound 2 group prepared by Production Method 2 with ethanol was the highest at 3.15 ± 1.09, and the preference of the female clathrate compound 1 group was the lowest at 2.08 ± 1.71.

In the taste item, the preference of Kwangju inclusion compound group 3 was the highest at 3.50 ± 1.81, and the preference of control group was lowest at 2.66 ± 1.70.

In the overall preference item, the preference of the female group 3 was the highest, 3.23 ± 0.71, and the preference of the control group was the lowest, 2.80 ± 1.22.

In summary, all bitter tastes were decreased in all groups of chewing clathrate compounds 1 and 3, and in particular, the chewing clathrate 3 group showed the highest preference in bitter taste, taste and overall acceptability.

Therefore, it can be concluded that the reaction of cyclodextrin to the concentrate of Yeoju can remove a certain amount of bitter taste specific to Yeoju and that the efficiency of removing bitter taste is the highest when the reaction is performed by adding water.

Claims (5)

A first step of preparing a solution by dissolving a predetermined amount of a yeast concentrate or a butterfly powder and a predetermined amount of cyclodextrin in a predetermined amount of 0.1 N acetic acid;
A second step of dropping 1.0 N sodium hydroxide (NaOH) so that the solution prepared in the first step has a pH of 10.0 to 12.0;
A third step of dropping 0.1 N hydrochloric acid (HCl) so that the solution prepared in the second step has a pH of 6.0 to 8.0;
Filtering the solution prepared in the third step, followed by lyophilization or spray drying.
delete delete A knee inclusion compound prepared by the method according to claim 1.
A composition comprising an effective inclusion compound prepared by the method according to claim 1 as an active ingredient.