KR101523600B1 - Skin-whitening composition and cosmetic composition comprising the same - Google Patents

Abstract

The present invention relates to a composition for skin whitening which inhibits melanin production, suppresses tyrosinase activity, is excellent in sulfation activity, and particularly has no cytotoxicity, and a cosmetic composition composition containing the same.
The composition for skin whitening and the cosmetic composition containing the same according to the present invention may contain methyl palmitate as an active ingredient.
Accordingly, the composition for skin whitening and the cosmetic composition containing the same according to the present invention have an effect of inhibiting the synthesis and delivery of melanin pigment, and exhibiting stain removal and whitening.

Description

(SKIN-WHITENING COMPOSITION AND COSMETIC COMPOSITION COMPRISING THE SAME)

The present invention relates to a composition for skin whitening and a cosmetic composition containing the same, and more particularly, to a composition for skin whitening which contains methyl palmitate as an active ingredient to inhibit synthesis and delivery of melanin pigment and remove stains, whitening, And a cosmetic composition containing the same.

In general, skin color of a person is mainly determined by the activity and distribution of melanocyte in the skin which forms melanocyte. Depending on the amount of pigment such as hemoglobin or carotenoid, skin thickness and reflectance, and blood flow . Melanin is a dihydroxyphenylalanine (DOPA) that is formed by an enzyme called tyrosinase contained in the melanocyte cell, which is a kind of amino acid normally present in the human body and exists in the basal layer of the epidermis. Through a series of complicated oxidation processes that are converted and continued to produce a dark brown pigment, the polymer melanin. Here, various internal and external environmental factors such as ultraviolet rays, drugs, hormones and genetic factors act as an influential factor of melanin synthesis. The melanin pigment is distributed widely to the surrounding keratinocyte through the dendrite of the melanocyte to determine the color of the skin.

Melanin, which is present in the outer skin layer of the skin, plays a role of ultraviolet ray shielding. It protects the subcutaneous skin organs and at the same time holds the active oxygen and free radicals generated in the skin of the skin. The protective role plays a useful role.

However, abnormal production of melanin causes skin lesions such as vitiligo. On the other hand, when melanin is not completely eliminated due to excessive synthesis of melanin due to sunlight, hormonal changes, inflammation, But also the pigment of the skin is deposited to form spots and freckles, and it also causes skin cancer from such lesions. Therefore, in order to prevent such pigment deposition phenomenon, it is necessary to inhibit a part of the melanin production process to reduce the production of melanin.

Today, oriental women prefer white, clean skin like white whiting and use it as an important criterion of beauty. Therefore, whitening agents are actively being developed to treat skin pigmentation abnormalities and meet beauty needs.

Currently, in the development of a whitening agent for skin cosmetics, there is known a method of decolorizing by reducing the produced melanin pigment and a method of inhibiting the activity of tyrosinase, an enzyme that forms a melanin pigment. However, it is known that the whitening agent using tocopherol, vitamins and the like used for reducing the melanin pigment has little discoloration effect of the melanin pigment.

Accordingly, inhibitors that inhibit the production of melanin pigment by attenuating the activity of tyrosinase have been attracting attention.

In the field of the conventional cosmetics, as a whitening component, for example, substances inhibiting tyrosinase enzyme activity such as kojic acid and arbutin, hydroquinone, L-ascorbic acid, Derivatives thereof and various plant extracts have been used.

However, the use thereof is limited due to poor stability in the prescription system and coloration, generation of odor, efficacy at a living body level, unclear effects, and safety problems. Kojisan inhibits the enzyme activity by adsorbing copper ions present in the active site of tyrosinase, but when used in cosmetics, instability, problems of skin side effects, and recent animal tests have proven that liver cancer has been induced, the use of cosmetic ingredients has been discontinued. Vitamin-C and its derivatives are difficult to use as a raw material for cosmetics because of its good oxidative instability. Hydroquinone has excellent whitening effect on skin, but it has allergic properties, toxicity to melanin-producing cells, permanent decolorization to skin It is highly irritating to the skin. It has recently been prescribed as a carcinogenic substance and its use is prohibited. In addition, arbutin is a derivative in which hydroquinone is conjugated with glucopyranoside. As a result, hydroquinone is less toxic to human body, and inhibits the synthesis of melanin pigment, resulting in an increase in melanin pigmentation However, it is disadvantageous in that it is partially decomposed by skin enzymes.

In addition to these whitening substances, research has been continuing to find whitening active ingredients in natural products, especially plants. From the standpoint of stability and possibility of discoloration, there are many problems in using cosmetics or medicines in excess of effective concentration. It does not even show.

Korean Patent Publication No. 10-2002-0011719 (published on February 09, 2002) Korean Registered Patent No. 10-0817191 (registered on Mar. 20, 2008)

The present invention provides a composition for skin whitening that inhibits melanin production, suppresses tyrosinase activity, is excellent in sulfation activity, and particularly has no cytotoxicity, and a cosmetic composition containing the same.

The present invention also provides a skin whitening composition and a cosmetic composition containing the same, wherein the composition contains methyl palmitate to inhibit synthesis and delivery of melanin pigment and has excellent stain removal and whitening.

The various problems to be solved by the present invention are not limited to the above-mentioned problems, and other problems not mentioned can be clearly understood by those skilled in the art from the following description.

The composition for skin whitening according to the present invention may contain methyl palmitate represented by the following formula (1) as an active ingredient.

[Chemical Formula 1]

The methyl palmitate can be used at a concentration of 10 nM to 1 uM.

In addition, the cosmetic composition according to the present invention can be prepared by including the composition for skin whitening.

The details of other embodiments are included in the detailed description and drawings.

The composition for skin whitening according to the present invention and the cosmetic composition containing the same inhibit melanin production, inhibit tyrosinase activity, exhibit excellent sulfation activity, and are particularly cytotoxic.

In addition, the composition for skin whitening and the cosmetic composition containing the same according to the present invention have an effect of suppressing the synthesis and delivery of melanin pigment and removing stains and whitening by containing methyl palmitate component.

It will be appreciated that embodiments of the technical idea of the present invention can provide various effects not specifically mentioned.

FIG. 1 is a graph showing that melanin pigment is synthesized in melanocytes of the lower part of keratinocyte of human skin and transferred to keratinocyte in the present invention.
FIGS. 2 to 7 are diagrams showing the results of an experimental example according to the present invention. FIG.

BRIEF DESCRIPTION OF THE DRAWINGS The advantages and features of the present invention, and how to accomplish them, will become apparent by reference to the embodiments described in detail below with reference to the accompanying drawings. However, the present invention is not limited to the embodiments described herein but may be embodied in other forms. Rather, the embodiments disclosed herein are provided so that the disclosure can be thorough and complete, and will fully convey the scope of the invention to those skilled in the art.

The terminology used in this application is used only to describe a specific embodiment and is not intended to limit the invention. The singular expressions include plural expressions unless the context clearly dictates otherwise. In this application, the terms "comprises", "having", and the like are used to specify that a feature, a number, a step, an operation, an element, a part or a combination thereof is described in the specification, But do not preclude the presence or addition of one or more other features, integers, steps, operations, components, parts, or combinations thereof.

Unless otherwise defined, all terms used herein, including technical or scientific terms, have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. Terms such as those defined in commonly used dictionaries are to be interpreted as having a meaning consistent with the meaning in the context of the relevant art and are to be construed as ideal or overly formal in meaning unless explicitly defined in the present application Do not.

Hereinafter, preferred embodiments of the skin whitening composition and the cosmetic composition containing the same according to the present invention will be described in detail with reference to the accompanying drawings.

The methyl palmitate used in the present invention is a compound having a chemical structure represented by the following Chemical Formula 1 and having a molecular weight of 270.46 and a chemical formula of C ₁₇ H ₃₄ O _{2 which} may also be referred to as N-hexadecanoic acid . The methyl palmitate is poorly soluble in water, and is well mixed with acetone, benzene, ether, alcohol, and chloroform, and has a specific gravity of 0.852. In general, methyl palmitate is used as a raw material for organometallic compounds to form intermediate products for making parasitic drugs, organic solutions and other chemicals. In the present invention, methyl palmitate can be used in a composition for skin whitening which inhibits synthesis and delivery of melanin pigment and has excellent stain removal and whitening, and a cosmetic composition containing the same.

[Chemical Formula 1]. Chemical structure of methyl palmitate

FIG. 1 is a diagram for showing that melanin pigment is synthesized in melanocytes in the lower part of the keratinocyte of the human skin and transferred to keratinocyte.

Referring to FIG. 1, methyl palmitate according to the present invention inhibits the synthesis and transfer of melanin pigment, has excellent stain removal and whitening, and can be used as a skin whitening composition. The melanin is a pigment present in the epidermal layer of the skin, which is made from melanocytes existing in the basal layer of the epidermis and migrates to epidermal cells called keratinocytes. In vivo, there is no enzymes that degrade melanin, but keratinocytes are removed from the skin as they fall off the skin.

In melanocyte synthesis, tyrosine is first converted to DOPAquinone via tyrosinase (DOPA), tyrosinase (tyrosinase), autoxidation reaction and enzymatic reaction from dopaquinone Through the reaction, the melanin, which is a dark brown copolymer, is produced through DOPAchrome. The resulting melanin is transferred to the keratinocyte through melanoma, and after about 28 days of keratinization, it exits the skin surface and disappears with the keratinocytes.

Melanin absorbs the light energy of sunlight UV and protects the subcutaneous skin organs from UV damage. It also helps protect the skin from external harmful factors such as harmful oxygen and free radicals generated in the skin. Role. In addition, the skin color of a person is determined according to the amount of melanin in the skin cells. People with high melanin pigment have brown or black skin while those with low melanin pigment have white skin.

Hereinafter, the skin whitening composition comprising methyl palmitate according to the present invention will be described in more detail with reference to the following experimental examples. The following experimental examples illustrate the invention in more detail but do not limit the scope of the invention.

<Experimental Example>

Referring to FIG. 2, methyl palmitate was treated with melanin cells at different concentrations, and then the pigment concentration of these cell pellets was visually confirmed.

3, it was confirmed that tyrosinase, an enzyme which plays an important role in the synthesis of melanin pigment, is reduced dose-doped by methyl palmitate. In addition, MITF Lt; RTI ID = 0.0 &gt; dose-doped. &Lt; / RTI &gt;

Referring to FIG. 4, tyrosinase enzyme concentration was measured and it was confirmed that it was decreased by methyl palmitate.

Referring to FIGS. 5 and 6, the toxicity of methyl palmitate was confirmed by MTT assay, and it was confirmed that it was not toxic at a concentration of 10 nM to 1 uM.

7, it was confirmed by FACS analysis that melanocytes and melanocytes were transferred from melanocytes to epidermal cells after co-culture and co-cultivation of melanocytes and epidermal cells, and treated with methyl palmitate , and whiter.

While the present invention has been described in connection with what is presently considered to be practical exemplary embodiments, it is to be understood that the invention is not limited to the disclosed embodiments, but, on the contrary, It will be understood that the invention may be practiced. It is therefore to be understood that one embodiment described above is illustrative in all aspects and not restrictive.

Claims (3)

A pharmaceutical composition comprising methyl palmitate having the following formula (1) as an active ingredient,
Wherein the methyl palmitate is used at a concentration of 10 nM to 1 uM.
[Chemical Formula 1]

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