KR101506863B1 - Skin care compositions for antiwrinkle effect comprising the extracts of Areca catechu L. and Juncus effusus var. decipiens Buchen - Google Patents

Abstract

The present invention relates to a cosmetic composition for improving skin wrinkles containing an extract of a skin extract and a sea mustard extract as an active ingredient. Wherein the cosmetic composition is one form selected from the group consisting of cosmetic liquids, essences, oils, creams, powders, packs, foundations, make-up bases and sticks, It is possible to give a synergistic effect to the main effect, preferably by containing another wound healing ingredient within a range that does not impair the main effect of the invention.

Description

[0001] The present invention relates to a cosmetic composition for improving skin wrinkles, comprising an extract of mulberry bark extract and an extract of mulberry leaf extract as an active ingredient. decipiens Buchen}

The present invention relates to a synergistic effect of the raw bean extract and the crude extract of collagen which has excellent effect of promoting the biosynthesis of collagen, which is excellent in the inhibitory effect on the elastase which directly acts on the skin wrinkle, The present invention is directed to a cosmetic composition for improving skin wrinkles.

The aging process occurs as human skin undergoes changes due to various internal and external factors as it gets older. Skin aging is largely divided into intrinsic aging caused by intrinsic factors and photoaging caused by extrinsic factors. Endogenous aging is a natural aging phenomenon that occurs as hormone secretion that regulates skin metabolism is reduced, immune cell function and cell activity are inhibited, biosynthesis of immune protein and bioprotein necessary for the living body is reduced, Is caused by the increase of free radicals and harmful free radicals on the skin due to the ultraviolet rays contained in the sunlight, and the skin wrinkles are accompanied by skin pathologies such as spots, freckles, and black spots.

In general, skin wrinkles are known to be caused by various factors such as the moisture content of the skin, the content of collagen, and collagen is known to have the greatest influence. Collagen is a fibrous tissue that gives skin flexibility. It is mainly produced in fibroblasts existing in the dermal layer of the skin, and is a major component that accounts for about 70% of dermal tissue. The polypeptide chains of collagen fibers are synthesized in fibroblasts and secreted out of the cells in the form of a pro-collagen combined with a triple helical structure. The secreted pro-collagens are combined with each other in the extracellular matrix And are synthesized as collagen fibers. The main functions of collagen are known as mechanical firmness of skin, resistance of connective tissues and binding force of tissues, support of cell adhesion, induction of cell division and differentiation. The synthesis of collagen fibers rapidly decreases as the skin ages, and the collagen fibers are deformed or decomposed by the external environment, and wrinkles are generated and deepened in the skin. The degradation of collagen involves collagenase, a collagenase. Collagenase is a calcium and zinc-dependent matrix metalloproteinase (MMP) secreted from cells such as polymorphonuclear neutrophils, macrophages, gingival fibroblasts, bone cells, etc., and acts at neutral pH , And various extracellular substrates as substrates. Such proteolytic enzymes are known to be involved in various physiological processes such as periodontal disease, arthritis, inflammation, diabetes, trauma and skin aging and wrinkles, and various diseases.

Elastin is an insoluble elastic fiber protein in animal connective tissue that maintains skin elasticity. It is a skin structure protein that plays a very important role in skin elasticity by forming crosslinks with collagen, though its amount is about 2%. Elastin is degraded by elastase. Therefore, it is known that the increase of the activity of elastase acts as an important factor in the wrinkle of the skin. Elastinase production is limited in the skin, while elastase is overproduced by an increase in age and skin irritation such as ultraviolet rays. Over-produced elastase degrades elastin excessively, which causes weakening of collagen bonds and causes skin wrinkles. Therefore, the inhibition of the activity of the elastase, an elastase, can fundamentally inhibit the generation of wrinkles in the skin. In order to delay skin aging, a method of compounding nutrients and additives in cosmetics has been used in the past, in which moisturizers, anti-inflammatory agents, elastin and collagen-crosslinked bonds are destroyed, which has a limitation in delaying aging. Therefore, there is a demand for an inhibitor which essentially inhibits the decomposition of elastin and collagen which maintain skin elasticity.

The bloody bloom ( Areca catechu L.) refers to the rind of the vineyard belonging to palmatella . The vineyard is a large tall tree with a height of 10 to 18 meters, and the egg-shaped hardened fruit is dark blue. When you cut the fruit, it is divided into a round seed (bingata) and a bark surrounding the seed (bingata). Alkaloids such as arecolin, alecidyl, and gubacholine are known to be effective ingredients in the case of bingo, but only a tannin is known only in the case of bamboo blood (Oriental pharmacology, Shinil Sangsaja, 2005, 548-549). In the study of efficacy, whitening efficacy and anti-aging efficacy were found in the case of bingo, but almost no studies on the efficacy of the bupivacaine were made. Thus, it was found that the bupivacaine induced hepatotoxicity in the rat induced by α-naphthylisothiocyanate There are reports that it is effective and that it acts on the digestive system and the nervous system. Prior studies on the effect of the skin on the skin of the skin have been reported in Korean Patent Publication No. 0742378 B1, atopic dermatitis control agent, Korean Laid-Open Publication No. 2008-0032127 hairdressing agent, and Korean Patent Publication No. 2011-0039403, There is little research on the efficacy of wrinkles such as wrinkles.

Juncus effusus var. Decipiens Buchen is a perennial herb that belongs to the genus Euphorbiaceae, and grows mainly in wetlands. It is used as a medicinal herb in August ~ September, and it cuts the stem and uses it only in the middle. It is known as a medicinal product with diuretic, antipyretic, sedative, and strengthening effects. The main ingredient is gluconuteoline, which is a flavonoid. Reports on the efficacy of jujubech have only reported on water purification, anxiolytic and soothing effects of sewage. Prior studies on the action of the sour cream on the skin include studies on the hair treatment composition of the self-immune disease treatment or inhibitory composition contained in the rush extract of Korean Registered Patent Publication No. 1113184 B1 and the hair care composition of the Korean Laid-Open Publication No. 2008-0032127, There is no study of efficacy.

Korean Registered Patent Publication No. 0742378 B1 Korean Patent Publication No. 2008-0032127 Korean Patent Publication No. 2011-0039403 Korean Patent Registration No. 1113184 B1 Korean Patent Publication No. 2008-0032127

Conventionally, products using collagen in cosmetics and the like have been marketed in order to utilize the skin moisturizing effect of collagen. However, these cosmetics are coated with collagen on the surface of the skin, and since the collagen, which is a polymer substance, I could not say that it is essential skin improvement. In order to solve this problem, there has been a growing interest in collagen synthesis promoting substances. Conventional collagen synthesis promoting substances include vitamin C, retinoic acid, transforming growth factor, (JP8-231370), betulinic acid (JP8-208424), chlorella extract (JP10-36283), and the like. However, the best known retinol is a substance that promotes collagen synthesis, but it is unstable. When applied to the skin, there is a limitation in usage due to stability problems such as irritation and seizure. The effect of chlorella extract is insignificant, Can not be expected. Therefore, it is urgently required to develop a cosmetic composition for improving skin wrinkles, which is safe and effective for a conventional skin wrinkle improving cosmetic composition botanical organism.

The cosmetic composition for improving the skin wrinkles according to the present invention contains an extract of a mulberry bark and a solution of a mulberry extract as an active ingredient.

And the content of the skin extract is in the range of 0.001 wt% to 10 wt% with respect to the weight of the composition. In addition, the watercress seed extract is blended in an amount of 0.001 wt% to 10 wt% with respect to the weight of the composition.

In detail, the safflower extract: Seaweed extract is compounded at a weight ratio of 1: 0.5 ~ 1.2.

In addition, the safflower seeds and roe mustard extract are fractions of water or a lower alcohol extract having 1 to 4 carbon atoms, and the lower alcohol extract is an extract obtained by cold extraction with methanol or ethanol for 3-7 days.

The cosmetic composition for improving skin wrinkles is one of the formulations selected from the group consisting of cosmetics, essences, oils, creams, powders, packs, foundations, makeup bases and sticks.

The present invention provides a cosmetic composition for improving wrinkles, characterized by containing a skin lotion and an extract of sea mustard extract as an active ingredient. The cosmetic composition according to the present invention may contain other wound healing ingredients within the range that does not impair the intended main effect of the present invention, thereby preferably synergizing with the main effect.

Fig. 1 is a graph showing the results of promoting collagen and elastin synthesis.

In order to achieve the above object, the present invention provides a cosmetic composition for improving skin wrinkles, which is characterized by maximizing the effect of improving skin wrinkles by simultaneously containing a skin extract having excellent collagen synthesis promoting activity and an extract of sea mustard extract, Lt; / RTI >

The preparation of the peeled pine needles and the extract of R. cinerea used in the present invention can be carried out by physically pulverizing the dried medicinal material and then treating it with a suitable solvent such as hot water, methanol, ethanol, butanol, chloroform, dichloromethane, hexane, ethyl acetate Extraction using a solvent, or supercritical extraction, and it is preferable to use a hot alcohol or a lower alcohol having 1 to 4 carbon atoms.

The cosmetic composition comprising the composition of the present invention as an active ingredient and the cosmetic composition of the present invention can be used for cosmetics for basic cosmetics or makeup such as lotion, essence, oil, cream, powder, foundation, foundation, makeup base, But the present invention is not limited thereto. Such a cosmetic composition can also be applied in various forms such as a liquid, a cream, a paste and a solid, and can be produced by a conventional cosmetic preparation method.

In addition, the components contained in the cosmetic composition of the present invention may contain an auxiliary component in addition to the above-mentioned skin extract and sour cream extract as an effective ingredient. Examples of auxiliary ingredients include preservatives, antioxidants, stabilizers, solubilizers, vitamins, pigments, fragrances or mixtures of the above.

The inventors of the present invention have studied the effect of inhibiting the activity on collagen fiber biosynthesis and elastase activity of natural and herbal extracts which are more friendly to human skin to solve the above problems, The present invention has been accomplished based on the discovery that the extract promotes the synthesis of collagen and the extract of Cichoraceae extract is excellent in the activity of inhibiting the activity against elastase, thereby excellently reducing the wrinkles caused by collagen and elastin deficiency. Based on the results of the experiment according to the present invention, it was confirmed that the cosmetic composition of the present invention surprisingly increases its effect when it is used as a mixture of peel and peanut extracts as compared with the case of using only peel or peanut extract alone.

Hereinafter, embodiments of the present invention will be described in detail in order to facilitate understanding of the present invention. However, the embodiments according to the present invention can be modified into various other forms, and the scope of the present invention is not limited to the following embodiments. Embodiments of the invention are provided to enable those of ordinary skill in the art to more fully understand the present invention.

(Example)

The crude fat was pulverized to a size of 100 mesh using a pulverizer, and then about 10 times (1:10 weight / volume) of methanol was added, followed by cold-extraction for 5 days and concentrated under reduced pressure using a rotary evaporator Respectively.

In the same manner as in the case of the above-mentioned dressing, the raspberry extract was prepared.

In the following Examples, the efficacy of the extracts and compounds according to the present invention was evaluated as follows.

(Example 1) Evaluation of cytotoxicity

Human fibroblasts pre-cultured with DMEM (Dulbecco's modified eagle medium) supplemented with 10% FBS (fetal bovine serum) were added to a 96-well plate at a concentration of 2 x ¹⁰ cells / ml and cultured in a CO ₂ incubator (37 ° C, 5% CO ₂ ) for 18 hours. The medium was removed, the diluted sample was added to the medium to which no FBS was added, and further cultured for 24 hours. 0.1 ml of MTT (3- (4,5-Dimethylthiazol-2-yl) -2,5-diphenyltetrazolium bromide, a yellow tetrazole) solution (2.5 mg / ml) was added thereto for 4 hours. DMSO (dimethyl sulfoxide) was added at a rate of 0.2 ml / well to dissolve the prepared formalin, and the absorbance at 570 nm was measured to evaluate the cytotoxicity of the extract.

The skin extracts and skin extracts obtained by the MTT method showed almost no toxicity to the skin fibroblasts at the maximum concentration of 50 μg / ml for the present invention. The results are shown in Table 1.

[Table 1]

(Example 2) Evaluation of promoting collagen synthesis (western blot)

Human fibroblasts pre-cultured with DMEM supplemented with 10% FBS were added to a 6-well plate at a concentration of 2 x ¹⁰ cells / ml and cultured in a CO ₂ incubator (37 ° C, 5% CO ₂ ) for 18 hours. The medium was removed and the diluted samples were added to the medium without FBS. After 48 hours of culture, the cells were lysed and analyzed by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), polyvinylidene fluoride or polyvinylidene difluoride (PVDF) Permeability, antibody binding, and the like.

The extracts of the hatchery extracts increased the production of type 1 collagen, which is a key component of the skin, in a concentration - dependent manner in fibroblasts derived from human skin. This can be seen from the results of FIG. From these results, it was confirmed that the extract from the skin of the seeds was useful for improving the wrinkles of the skin.

(Example 3) An enzyme-linked immunosorbent assay (ELISA)

Human fibroblasts pre-cultured with DMEM supplemented with 10% FBS were added to a 24-well plate at a concentration of 2 x ¹⁰ cells / ml and cultured in a CO ₂ incubator (37 ° C, 5% CO ₂ ) for 18 hours. The medium was removed, and a sample diluted in FBS-free medium was added. After 48 hours of culture, the culture broth was subjected to quantitative analysis of type 1 collagen type I collagen. Collagen quantification was performed using a Procollagen type 1 EIA kit (Takara, Japan).

The effect of the present invention on the production of collagen type I in fibroblasts was evaluated by ELISA, and the results are shown in Table 2.

[Table 2]

As shown in Table 2 above, the extract from the skin of the control group increased the production of type 1 collagen in a concentration-dependent manner in fibroblasts derived from human skin.

(Example 4) Elastase activity inhibiting ability evaluation

EnzCheck Elastase assay kit (InVitrogen, USA) was used to evaluate the inhibitory activity of elastase activity. DQ ^TM elastin (obtained from bovine neck ligament) and fluorescein conjugate were used as substrates. That is, 110 μl of reaction buffer, 20 μl of sample, 50 μl of elastase (1 μg / ml) and 30 μl of elastin (100 μg / ml) were added to a 96-well plate and incubated at 37 ° C for 1 hour And the experimental results were measured at 505/515 nm using a fluorescence microplate reader. N-Methoxysuccinyl-Ala-Ala-Pro-Val-chloromethylketone was used as a positive control. The inhibition rate of the enzymatic activity of elastase was determined by the following equation.

Enzyme inhibition rate (%) = 100 - (Experimental enzyme activity / control enzyme activity X 100)

The enzymatic assay kit was used to evaluate the effect of the extracts of the bean curd refuse and the raspberry extract on elastase enzyme inhibition. The results are shown in Table 3.

[Table 3]

From the above results, it was found that the extract of Codonopsis lanceolata generally has an inhibitory effect on elastase than doxycycline used as an inhibitor of elastase activity in medicines. At the same time, the simultaneous use of both the extracts of the seeds and the rumen extract resulted in superior efficacy of inhibiting elastase activity and synergistic effects. In addition, the inhibition rate of elastase enzyme increased sharply as the content of each of the crude extract and the sourdough extract increased. It is preferable that the crude extract and the raspberry extract are mixed at a ratio of 1: 0.5 to 1.2 parts by weight, preferably 1: 0.9 to 1.1 parts by weight. As a result, the result that the skin extract of bean curd refuse can promote the elastase activity inhibitory effect of the sea mustard extract was novel and surprising.

(Example 5 and Comparative Example 1) Experiment on skin wrinkle improvement effect through panel test

For the makeup bases of the following examples and comparative examples, the effect of improving skin wrinkles was tested as follows for healthy, 40 to 60 year old women. Twenty - two female subjects were exposed to the right facial part and the left facial part for 2 months once a day. After two months, the degree of improvement of the wrinkles of the skin was evaluated by the experiment of the subject. The questionnaire of the subjects was judged to have no improvement, slight improvement, moderate improvement, and considerable improvement as compared with before use with regard to skin wrinkle improvement and elasticity enhancement.

The makeup base prescription examples used in Example 5 and Comparative Example 1 are shown in Table 4 below.

[Table 4]

As shown in the following Table 5, Example 5, which contained the extracts of mulberry bark extract and mulberry extract, exhibited a slight improvement compared with Comparative Example 1, thereby exhibiting a wrinkle-healing effect. In contrast, most of the subjects in the comparative example, except for a few, could not feel improvement. In addition, neither Example 5 nor Comparative Example 1 showed skin irritation or side effects during the two month experimental period. From these results, it was confirmed that the cosmetic composition comprising the mixture of the crude extract and the water extract of the rumen extract showed an effect of improving skin wrinkles.

[Table 5]

Claims (7)

The cosmetic composition for improving skin wrinkles comprising as an active ingredient, an extract of a mulberry extract and a solution of a mulberry extract, wherein the mulberry extract has a weight of 0.001 wt% to 0.005 wt% based on the weight of the composition, % To 0.005% by weight of the composition. The composition of the present invention comprises: 1: 0.5 to 1.2;
delete delete delete The method according to claim 1,
The cosmetic composition for improving skin wrinkles is characterized by being a fraction of water or a lower alcohol extract having 1 to 4 carbon atoms.
6. The method of claim 5,
Wherein the lower alcohol extract is an extract obtained by cold extraction with methanol or ethanol for 3 to 7 days.
The method according to claim 1, 5, or 6,
Wherein the cosmetic composition is one form selected from the group consisting of cosmetic liquids, essences, oils, creams, powders, packs, foundations, make-up bases and sticks.

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