KR101489461B1 - Buffy coat extraction kit for self blood renewal procedure - Google Patents

Abstract

A buffy coat extraction kit for autologous blood skin regeneration is disclosed. A buffy coat extraction kit for autologous blood skin regeneration according to an embodiment of the present invention includes a plasma part in which a plasma layer is formed when blood is centrifuged, a buffy coat formed in a bottom part of the plasma part, A kit body including an erythrocyte section extending from a lower end of the buffy coat section to form a red blood cell layer and a piston engaging section extending from a lower end of the erythrocyte section; A locking device coupled to the open top of the plasma portion of the kit body to prevent leakage of blood and prevent reuse of the kit body; And a piston which is coupled to a piston coupling portion of the kit body and is reciprocatable in a linear direction along the longitudinal direction of the kit body to adjust the blood receptor volume within the kit body.

Description

Technical Field [0001] The present invention relates to a BUFFY COAT EXTRACTION KIT FOR SELF BLOOD RENEWAL PROCEDURE,

The present invention relates to a buffy coat extraction kit for autologous blood skin regeneration.

Recently, autologous blood cell regeneration (Platelet Rich Plasma, also referred to as "platelet-rich plasma", hereinafter referred to as "PRP procedure") has been spotlighted in the practice of extracting PRP from its own blood, And treatment of wound by regenerating cell tissue by injection. The PRP procedure is widely applied to treatments such as regenerating injured cartilage and regenerating damaged skin tissue such as acne scars to improve the skin condition.

It is known that platelets of blood are rich in growth factors. These platelets are known to multiply cells in the human body, produce collagen, regenerate new blood vessels and heal wounds.

Human blood contains about 100,000 to 200,000 platelets per microliter. For PRP to be effective, platelets must be concentrated to include more than one million platelets per microliter.

As such a method of concentrating platelets, a method of separating the collected blood into layers using a centrifuge is used. When blood is separated into layers by a centrifuge, the red blood cells Yellow buffy coat layer composed of white blood cells and platelets is formed on the red blood cell layer and a light yellow plasma layer made of plasma on the top of the buffy coat layer.

The volume ratio of the separated blood layers is 45% on the average, 1% on the buffy coat, and 54% on the plasma layer. In the PRP procedure, a platelet-rich buffy coat layer is extracted from the separated blood as described above, and the ratio is only 1%.

The background art of the present invention is disclosed in Korean Patent Registration No. 10-1140551 (Registered on Apr. 20, 2012, Buffy coat extraction kit for autologous blood skin regeneration).

The embodiment of the present invention can easily extract the buffy coat in which blood platelets are concentrated in the blood separated by the centrifugal separator for the PRP procedure and reuse the kit body to prevent medical accidents due to reuse of the used kit body And to provide a buffy coat extraction kit for autologous blood skin regeneration.

According to an aspect of the present invention, there is provided a blood coagulation apparatus comprising: a blood plasma section in which a blood plasma layer is formed when blood is centrifuged; a buffy coat section extending from a lower end of the plasma section to form a buffy coat layer; A kit body including an erythrocyte section in which a red blood cell layer is formed and a piston engaging section extending from a lower end of the erythrocyte section; A locking device coupled to an open upper end of the blood plasma of the kit body to prevent leakage of blood and prevent reuse of the kit body; And a piston which is coupled to the piston coupling portion of the kit body and is reciprocatable linearly along the longitudinal direction of the kit body to adjust the blood receptor volume within the kit body, Can be provided.

The locking device comprising: a locking cap having a through hole and coupled to wrap the open top of the plasma section; And an automatic sealing rubber that is coupled to the through-hole of the locking cap so that blood can be injected and extracted using a syringe.

The locking cap may include a closed end projecting in a bent shape from an edge of the locking cap to surround an outer peripheral surface and an inner peripheral surface of an open upper end of the plasma part, and an open upper end of the plasma part is coupled with the shield end, And may be formed in a shape corresponding to the closed end so that a sealing gap is formed.

A first protrusion protruding from the edge of the locking cap toward the plasma part to surround the outer peripheral surface of the open upper end of the plasma part; And a second protrusion protruding from the edge of the locking cap to the plasma part side and positioned inside the first protrusion part so as to surround the inner peripheral surface of the open upper end of the plasma part.

A groove may be formed along the periphery of the inner side surface of the first projecting portion opposed to the outer peripheral surface of the open upper end of the plasma portion, and a projection having a shape corresponding to the groove is formed on the outer peripheral surface of the open top portion of the plasma portion. Can be formed.

A groove may be formed along an outer circumference of the outer surface of the second protrusion opposite to an inner circumferential surface of the open upper end of the plasma section, and an inner circumferential surface of the open upper end of the plasma section may have a protrusion Can be formed.

The piston may include a cup shape having a groove formed in the longitudinal direction from the upper end to the lower end to receive the blood.

The piston may include a screw portion formed on an outer circumferential surface of the piston so as to be screwed with the piston engaging portion of the kit body.

The piston may further include a rotating groove formed at a lower end of the piston so that the piston is rotated using a rotating tool.

The buffy coat extraction kit for autologous blood skin regeneration may further include a disk type coupling unit formed on the outer circumferential surface of the kit body so that the kit body is fixed to the centrifuge.

According to the embodiment of the present invention, it is possible to easily extract a buffy coat in which platelets are concentrated in blood separated by a centrifuge for PRP.

Also, it is possible to prevent medical accident caused by reuse of the kit body once used by preventing the detachment of the locking device coupled to the open upper end of the kit body.

In addition, even when the composition ratio in the blood is different, it is possible to accurately extract the buffy coat, and the amount of the blood to be collected so that the buffy coat can be extracted according to the blood volume of the collected blood, that is, the blood receptor volume inside the kit body, have.

FIG. 1 is a partial perspective view of a buffy coat extraction kit cut vertically, for an autologous blood skin regeneration procedure according to an embodiment of the present invention. FIG.
FIG. 2 is a perspective view illustrating a buffy coat extraction kit for an autologous blood skin regeneration procedure according to an embodiment of the present invention. FIG.
FIG. 3 is a view showing a locking device of a buffy coat extraction kit for an autologous blood skin regeneration procedure according to an embodiment of the present invention shown in FIG. 1;
FIG. 4 is a cross-sectional view illustrating a coupling relationship between the locking device of the buffy coat extraction kit and the kit body for the autologous blood skin regeneration procedure according to the embodiment of the present invention shown in FIG.
5 is a view illustrating a process of injecting blood drawn into a buffy coat extraction kit for an autologous blood skin regeneration procedure according to an embodiment of the present invention.
6 is a view showing the layered components of blood formed in the buffy coat extraction kit for the autologous blood skin regeneration procedure according to the embodiment of the present invention after centrifugal separation by a centrifugal separator.
FIG. 7 is a view illustrating a process of extracting a buffy coat from a blood drawn using a buffy coat extraction kit for an autologous blood skin regeneration procedure according to an embodiment of the present invention.

BRIEF DESCRIPTION OF THE DRAWINGS The present invention is capable of various modifications and various embodiments, and specific embodiments are illustrated in the drawings and described in detail in the detailed description. It is to be understood, however, that the invention is not to be limited to the specific embodiments, but includes all modifications, equivalents, and alternatives falling within the spirit and scope of the invention. DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS Hereinafter, the present invention will be described in detail with reference to the accompanying drawings.

DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENT Hereinafter, embodiments of the present invention will be described in detail with reference to the accompanying drawings. Referring to the accompanying drawings, the same or corresponding components are denoted by the same reference numerals, do.

FIG. 1 is a partial perspective view of a buffy coat extraction kit for an autologous blood skin regeneration procedure according to an embodiment of the present invention, FIG. 2 is a perspective view showing a buffy coat extraction kit for the present invention. FIG.

As shown in FIGS. 1 and 2, the buffy coat extraction kit 1000 for autologous blood skin regeneration according to an embodiment of the present invention collects blood for buffy coat A locking device 200 and a piston 300. The kit body 100 includes a locking device 200,

According to this embodiment, a buffy coat in which platelets are concentrated in blood separated by a centrifuge for PRP treatment can be easily extracted.

For reference, these medical devices are disposable devices that should not be reused. However, in the medical field, the reuse of these devices is frequent in the name of cost reduction, which is likely to cause unintended medical accidents.

According to the present embodiment, since the cap which is coupled to the open upper end of the kit body 100 to seal the kit body 100 is structured with difficulty in separation once it is once coupled, Medical accidents can be prevented in advance.

In addition, conventionally, a container (i.e., a kit body) corresponding to the capacity of the collected blood was used in accordance with the capacity of the collected blood, so that containers corresponding to respective capacities such as 10, 12, and 15 milliliters I needed to do it.

However, according to the present embodiment, it is possible to always accurately extract the buffy coat even if the composition ratio in the blood is different, and it is possible to accurately measure the amount of blood to be drawn so as to extract the buffy coat according to the blood volume, The blood receptor dose can be adjusted according to the needs of the user.

Therefore, according to the present embodiment, it is possible to adjust the blood receptor volume inside the kit body 100 in accordance with the volume of the blood collected using one kit body 100, Thereby reducing costs.

Hereinafter, a specific configuration of the buffy coat extraction kit 1000 for the autologous blood skin regeneration procedure according to the present embodiment will be described in detail with reference to FIG. 1 to FIG.

The kit body 100 includes a plasma section 110 in which a blood plasma layer is formed when the blood injected into the kit body 100 is layered and separated using a centrifuge (not shown) A red blood cell portion 130 extending from the lower end of the buffy coat portion 120 to form a red blood cell layer and a red blood cell portion 130 extending to the lower end of the red blood cell portion 130. The buffy coat portion 120, And a piston coupling portion 140 formed on the piston coupling portion 140.

As shown in FIG. 1, the kit body 100 may have a shape in which a central portion penetrates in the longitudinal direction to form a hollow. The kit body 100 may be made of a transparent material so that the layered components of the blood can be observed from the outside after separating the collected blood with a centrifuge.

In this embodiment, when the collected blood is centrifuged in consideration of the composition ratio of erythrocytes, buffy coat and plasma of normal blood, the kit body 100 is formed so that a buffy coat layer is formed at an intermediate portion of the buffy coat 120, The inner diameter and the length of the blood plasma section 110, the buffy coat section 120, and the erythrocyte section 130 may be different from each other.

In this case, the inner diameter of the buffy coat part 120 is relatively narrowed and the length thereof is made long, so that when the collected blood is layered on the centrifugal separator, the buffy coat layer having a relative volume ratio of only about 1% Can be positioned in the area of the narrow and long buffy coat 120, so that the extraction of the buffy coat can be easily performed.

According to this embodiment, as shown in FIG. 1, the plasma part 110 may have a cylindrical shape with a large inner diameter.

The buffy coat 120 extending downward from the lower end of the plasma part 110 may be formed in a cylindrical shape having a smaller inner diameter by reducing the inner diameter of the plasma part 110.

The erythrocyte section 130 extending downward from the lower end of the buffy coat section 120 may be formed in a cylindrical shape having a large diameter extending from the inner diameter of the buffy coat section 120.

The lower end of the red blood cell part 130 may be provided with a piston coupling part 140 to which a piston 300 described later is coupled.

In this case, the connection section between the plasma section 110 and the buffy coat section 120 and the connection section between the buffy coat section 120 and the erythrocyte section 130 are curved such that their inner diameters gradually change as shown in FIG. Shape. As a result, the flow of blood in the kit body 100 can be performed well, so that the layered separation can be easily performed for each component.

On the other hand, a thread may be formed on the inner surface of the piston coupling part 140, and the diameter of the thread may be formed to be equal to or larger than the inner diameter of the erythrocyte part 130. Therefore, the upper end of the piston 300, which will be described later, can be inserted into the erythrocyte section 130 through the inside of the piston coupling section 140.

FIG. 3 is a view schematically showing a locking device 200 of a buffy coat extraction kit 1000 for an autologous blood skin regeneration procedure according to the present embodiment shown in FIG. 1. FIG. 4 is a cross- Sectional view showing a coupling relationship between the locking device 200 and the plasma part 110 of the buffy coat extraction kit 1000 for the autonomous blood skin regeneration procedure according to the embodiment.

The locking device 200 is a member coupled to the open upper end of the blood plasma part 110 of the kit body 100 to prevent leakage of blood and prevent reuse of the kit body 100.

To this end, the locking device 200 includes a locking cap 210, which is coupled to surround the open upper end of the plasma section 110, a through hole 210 of the locking cap 210, And an auto-sealing rubber 220 coupled to the body 211.

In this case, the lock cap 210 may be made of a hard plastic material, and the self-sealing rubber 220 may be made of a soft material such as silicone.

3, the lock cap 210 may have a circular stopper shape when viewed from above. The lock cap 210 may be made of a transparent material, and a through hole 211 may be formed at the center of the lock cap 210 so as to penetrate the upper and lower portions so that the automatic sealing rubber 220 can be fastened.

The lock cap 210 may be formed with a pressure adjusting hole 212 passing through the upper and lower sides so as to be adjacent to the through hole 211.

3, the through-hole 211 and the pressure adjusting hole 212 are formed in the through-hole 211 of the automatic stopper rubber 220 (see FIG. 3) So that leakage of blood in the kit body 100 can be prevented.

5 and 7, the lock cap 210 is provided in the kit body 100 by using a syringe, by providing the lock cap 210 with the pressure adjusting hole 212, which is normally sealed by the automatic sealing rubber 220, The air pressure in the kit body 100 can be adjusted when the blood is injected or extracted in the opposite direction, so that blood can be injected or extracted more easily as a result.

3 shows a case where a pair of pressure adjusting holes 212 are disposed on both sides of the through hole 211. However, the present invention is not limited to this, and the pressure adjusting holes 212 ) Can be varied in various ways.

The self-sealing rubber 220 may be made of rubber having a high expansion index and viscosity, such as silicone rubber. Protrusions (not shown) are formed on the upper surface of the self-sealing rubber 220 at positions opposing the through holes 211 and the pressure adjusting holes 212 of the lock cap 210 as shown in FIGS. 3 and 4 . By forming protrusions on the upper surface of the automatic sealing rubber 220 facing the through holes 211 and the pressure adjusting holes 212, blood can be prevented from leaking.

According to the present embodiment, the lock cap 210 includes a closed end 215 protruding downward from the edge of the lock cap 210 to cover the open top end and the inner circumferential end of the plasma part 110 can do. In this case, the open upper end of the plasma section 110 may be formed in a shape corresponding to the closed end 215 so as to be combined with the closed end 215 to form a curved sealing gap.

Referring to FIG. 4, the closed end 215 may include a first protrusion 216 and a second protrusion 217.

3 and 4, the first protrusion 216 protrudes from the edge of the lock cap 210 to the open upper side of the plasma section 110 to cover the outer peripheral surface of the open upper end of the plasma section 110 . ≪ / RTI >

The second projection 217 may protrude from the edge of the locking cap 210 to the open top side of the plasma section 110 and may wrap around the inner peripheral surface of the open top of the plasma section 110. In this case, the second projection 217 may be positioned inside the first projection 216 with respect to the central axis of the lock cap 210.

That is, grooves (unidentified marks) may be formed by the first projections 216 and the second projections 217, and the upper ends of the plasma units 110 may be inserted into these grooves. In this embodiment, the depth of the groove formed by the first protrusion 216 and the second protrusion 217 may be 3 millimeters.

4 is formed on the inner surface of the first protrusion 216 opposed to the outer peripheral surface of the open upper end of the plasma section 110 in order to firmly couple the lock cap 210 and the plasma section 110. [ The groove 218 may be formed in a ring shape along the inner circumference of the first protrusion 216 as shown in FIG.

In this case, protrusions 111 having a shape corresponding to the groove 218 can be formed in a ring shape on the outer peripheral surface of the opened upper end of the plasma part 110, so that the upper end of the plasma part 110 is in contact with the closed end 215 ) To form a curved sealing gap.

Therefore, after the upper end of the plasma section 110 is inserted between the first projection 216 and the second projection 217, the edge of the locking cap 210 is prevented from being opened by an external force, Reuse can be blocked in advance.

As described above, according to the present embodiment, since the cap for sealing the kit body 100, which is coupled to the open upper end of the kit body 100, It is possible to prevent a medical accident caused by the reuse of the device.

Although the groove 218 is formed on the inner surface of the first protrusion 216 and the protrusion 111 is formed on the outer circumferential surface of the plasma section 110 in the present embodiment, And the positions of the grooves 218 and the protrusions can be changed as needed.

4, the groove 218 is formed along the periphery of the outer surface of the second projection 217 opposite to the inner peripheral surface of the open upper end of the plasma section 110. For example, And the projections 111 may be formed in a ring shape on the inner peripheral surface of the open upper end of the plasma section 110 corresponding to the shape of the groove 218. [

FIGS. 5 to 7 illustrate a process of extracting a buffy coat from blood collected using the buffy coat extraction kit 1000 for the autologous blood skin regeneration procedure according to the present embodiment.

FIG. 5 shows a process of injecting blood drawn into the buffy coat extraction kit 1000 for the autologous blood skin regeneration procedure according to the present embodiment. FIG. 6 shows centrifugal separation by a centrifugal separator, FIG. 7 schematically shows a layered component of blood formed in the buffy coat extraction kit 1000 for autologous blood skin regeneration according to an embodiment of the present invention. FIG. 7 illustrates a buffy coat extraction kit 1000 for autologous blood skin regeneration according to the present embodiment. And extracting the buffy coat from the blood obtained by using the blood.

The piston 300 is coupled to the piston coupling portion 140 of the kit body 100 and is capable of reciprocating linearly along the longitudinal direction of the kit body 100 to adjust the blood receptor volume within the kit body 100.

Specifically, the piston 300 may be formed in a cup shape having a groove formed from the upper end to the lower end along the length direction of the piston 300 to receive blood.

The cup-shaped groove thus formed can accommodate more blood than conventional. Accordingly, it is possible to adjust the blood volume of the user to one using the kit body 100 without having to individually provide containers corresponding to the respective capacities, such as 10, 12, and 15 milliliters, depending on the blood volume.

5, the piston 300 may be coupled to the piston coupling part 140 and linearly reciprocated along the longitudinal direction of the kit body 100 to extract the buffy coat according to the volume of collected blood, The amount of the blood injected into the kit body 100, that is, the blood receptor dose within the kit body 100, can be adjusted according to the needs of the user.

The piston 300 may include a threaded portion 310 formed on the outer circumferential surface of the piston 300 so as to be screwed with the piston coupling portion 140 of the kit body 100.

The threaded portion 310 of the piston 300 can be screwed into a thread formed inside the piston coupling portion 140 of the kit body 100 so that it can be advanced or retracted along the longitudinal direction of the kit body 100.

In this case, a sealing material such as an o-ring may be provided on the outer circumferential surface of the upper end of the piston 300 to prevent blood from leaking through the portion where the piston 300 and the piston coupling portion 140 meet.

The piston 300 may further include a rotation groove 320 formed at the lower end of the piston 300 to rotate the piston 300 using a rotating tool (not shown). The rotation groove 320 may have various shapes, for example, a straight shape, a round shape, or a star shape.

Therefore, according to the present embodiment, it is possible to adjust the blood receptor volume inside the kit body 100 in accordance with the volume of the blood collected using one kit body 100, Thereby reducing costs.

The buffy coat extraction kit 1000 for autologous blood skin regeneration according to the present embodiment includes a disk-shaped coupling 100 formed on the outer peripheral surface of the kit body 100 so that the kit body 100 is fixed to a centrifuge (not shown) (400). ≪ / RTI >

The disk-shaped coupling portion 400 may have a disk shape extending radially outward from the vertical center axis (imaginary axis) of the kit body 100 as shown in FIG.

In the case of this embodiment, a pair of disc-shaped engaging portions 400 may be installed in parallel to each other, and a bucket jig (not shown) of a centrifugal separator (not shown) Can be inserted and fixed. Therefore, even if the kit body 100 rotates at high speed, breakage or departure due to shaking can be prevented.

The procedure for extracting the buffy coat in blood using the buffy coat extraction kit 1000 for the autologous blood skin regeneration according to this embodiment is as follows. As shown in FIG. 5, The blood is injected into the buffy coat extraction kit 1000 for the skin regeneration procedure, and the blood is separated into layers by mounting the buffy coat extraction kit 1000 to the centrifuge after removing the syringe and performing autologous blood skin regeneration.

6, the buffy coat layer is applied to the buffy coat portion 120 of the kit body 100 of the buffy coat extraction kit 1000 for autologous blood skin regeneration, . Then, as shown in FIG. 7, the buffy coat can be extracted by making the needle tip of the syringe reach the buffy coat layer.

On the other hand, when erythrocytes are abnormally large or small in the blood, the buffy coat layer can be formed at a position off the buffy coat 120 (in the figure, the number of red blood cells is small) So that the buffy coat layer can be positioned in the buffy coat 120 by advancing or retracting the buffy coat layer. Then, as shown in FIG. 7, the needle tip of the syringe can be brought to the buffy coat layer to extract the buffy coat.

While the present invention has been particularly shown and described with reference to exemplary embodiments thereof, many modifications and changes may be made by those skilled in the art without departing from the spirit and scope of the invention as defined in the appended claims. The present invention can be variously modified and changed by those skilled in the art, and it is also within the scope of the present invention.

1000: Buffy coat extraction kit for autologous skin regeneration
100: kit body
110: plasma part 111: projection
120: Buffy coat part 130: Red blood cell part
140: piston coupling part 200: locking device
210: locking cap 211: through hole
212: Pressure regulating hole 215:
216: first protrusion 217: second protrusion
218: groove 220: automatic sealing rubber
300: piston 310:
320: rotating groove 400: disk-shaped engaging portion

Claims (10)

A buffy coat part extending from a lower end of the plasma part to form a buffy coat layer; a red blood cell part extending from a lower end of the buffy coat part to form a red blood cell layer; And a piston engaging portion extending from a lower end of the erythrocyte portion;
A locking device coupled to an open upper end of the blood plasma of the kit body to prevent leakage of blood and prevent reuse of the kit body; And
And a piston which is coupled to a piston coupling portion of the kit body and is reciprocatable linearly along a longitudinal direction of the kit body to adjust the volume of the blood receptor inside the kit body,
Wherein the locking device comprises a locking cap having a through hole and coupled to wrap the open top of the plasma section,
Wherein the locking stopper protrudes in a bent shape from an edge of the locking stopper and surrounds an outer circumferential surface and an inner circumferential surface of the open upper end of the plasma unit,
Wherein an open upper end of the plasma section is formed in a shape corresponding to the closed end so as to form a bent sealing gap by being coupled with the shield end,
The above-
A first protrusion protruding from the edge of the locking cap toward the plasma part and surrounding an outer peripheral surface of the open upper end of the plasma part; And
And a second protrusion protruding from the edge of the locking cap to the plasma part side and located inside the first protrusion part so as to surround the inner peripheral surface of the open upper end of the plasma part,
Wherein the piston has a cup shape formed in a longitudinal direction along the longitudinal direction from an upper end to a lower end to receive blood.
The method according to claim 1,
The locking device comprises:
And a self-sealing rubber joined to the through-hole of the locking cap so as to be able to inject and extract blood using a syringe.
delete delete The method according to claim 1,
A groove is formed along the periphery of the inner side surface of the inner side surface of the first projecting portion opposed to the outer peripheral surface of the open upper end of the plasma portion,
Wherein a protrusion having a shape corresponding to the groove is formed on an outer peripheral surface of the open upper end of the blood plasma part.
The method according to claim 1,
A groove is formed in the outer surface of the second projection opposite to the inner peripheral surface of the open upper end of the plasma part along the periphery of the outer surface,
And a protrusion having a shape corresponding to the groove is formed on an inner peripheral surface of the open upper end of the blood plasma part.
delete The method according to claim 1,
Wherein the piston includes a threaded portion formed on an outer circumferential surface of the piston so as to be screwed with the piston engagement portion of the kit body.
9. The method of claim 8,
Wherein the piston further comprises a rotating groove formed at a lower end of the piston so that the piston is rotated using a rotating tool.
The method according to claim 1,
Further comprising a disc-shaped engaging portion formed on an outer circumferential surface of the kit body so that the kit body is fixed to the centrifugal separator.

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