KR101487224B1 - Composition for protecting against jellyfish stings - Google Patents

Abstract

The present invention relates to a composition for repelling a jellyfish. It was confirmed that activation of an Acanthopanax koreanum extract and/or a Cinnamomum camphora extract inhibits cnidocytes of the jellyfish from discharging toxin, and disclosed is a composition for repelling a jellyfish using the same. The composition comprises one or metal positive ions selected from a group consisting of gallium, calcium, magnesium, sodium, zinc, iron, gadolinium, potassium, titanium, and cobalt.

Description

TECHNICAL FIELD [0001] The present invention relates to a composition for protecting jellyfish,

The present invention relates to a composition for combating jellyfish, and more particularly, to a composition for combating jellyfish containing an islet extract and / or a camphor extract as an active ingredient.

Jellyfish are larval jellyfish that live in the ocean and are formed by the polyps. Since the 1960s, massive outbreaks of jellyfish have appeared and the damage has begun to occur. The cause of mass production is known as global warming, deepening of marine pollution, construction of offshore structure, depletion of fishery resources, In addition, societal and economic damage has spread throughout the world, including Japan and Southeast Asia. In addition, there are reports of large-scale jellyfish appearing in China and Japan adjacent to Korea, and personnel accidents due to skin contact have also occurred (Fenner et al., Thesis, University of London, 1997. 205.; Kawahara et al., Marine Ecology Progress Series 2006.307: 161-173.).

Massive outbreaks of jellyfish are powerful predators, which can be an important problem in terms of disturbing the bases of marine ecosystems, as well as socioeconomic activities directly linked to humans, such as the operation of nuclear power plants and fisheries activities, And jellyfish stinging accidents are constantly increasing and becoming a lot of problems. In 2009, the amount of settlement of damages such as nuclear power plants and fisheries exceeded 200 billion won, and the number of jellyfish stings increased by more than five times from 123 in Busan in 2008 to 634 in 2009 (Busan Metropolitan City Disaster Response Division ; Jung, Mi-hee et al., Journal of the Korean Society of Oceanography, 2012 17 (1) 25-31).

On the surface of the tentacles of the jellyfish are cnidocyte, which, when caught for food or at risk from predators, releases the poison that it has in the nematocyst. Japorotoxin is usually high in temperature and poisonous is strongest before maturation of jellyfish gonads. The jellyfish is dried with the net attached to it, and when it is blown by the wind, it gets red when it enters the eye, and when it enters the nose, it stimulates the mucous membrane in the nose. When it is stuck in jellyfish, it may cause skin pain accompanied by fever, urticaria, and watery. When it is shot on some toxic jellyfish, it is accompanied by vomiting and sweating, chest cavity, abdominal pain and severe case of pulmonary edema resulting in death (Uye S. Plankton and Benthos Research, 2008. 3: 125-131 .; Richardson AJ et al., Trends in Ecology & Evolution 2009, 24: 312-322). In addition to jellyfish, animals belonging to hydrangeas such as hydra and coral reefs give off the poison poison. The jellyfish has the greatest damage due to the constant floating in the water. Jellyfish are currently appearing in our country offshore is no-bunch ipgit jellyfish, moon water, jellyfish, luminous deep-sea jellyfish, including over 31 paper and (Agriculture, Forestry and Fisheries, 2011 Jellyfish occur trends and damage prevention measures and policy papers) Among luminous deep-sea jellyfish (Pelagia noctiluca ) is mainly used as an animal model for the study of jellyfish toxicity due to its strong toxicity.

US Patent No. 5958397 discloses a technique for reducing jellyfish stings by using polyquaternium in emulsion or lotion form. This method is effective for reducing damage by suppressing jellyfish stings itself, European Patent Publication No. 01511457 discloses a jellyfish elimination technique in which Ga + 3 and La + 3 are mixed. European Patent No. 00984776 discloses a technique for eliminating jellyfish mixed with antihistamine, magnesium and iron, but current demand for the product has been increasing.

As the frequency of tropical toxic jellyfish is continuously increasing due to the continuous warming and eutrophication of seawater, the population enjoying marine leisure activity is expected to increase. Therefore, the jellyfish eliminator research and development This is urgent.

It is an object of the present invention to provide a composition for combating jellyfish which comprises an extract of Ischia pertussis and / or a camphor tree extract as an active ingredient.

Other and further objects of the present invention will be described below.

As shown in the following Examples and Experimental Examples, the present inventors examined the number of discharges of nematocysts after stimulation with an extract of anthocyanin and camphor extract, respectively, The inhibitory activity of the extracts against the shoots was confirmed. The jellyfish which exists in specific cells of the jellyfish tentaculum is a thread structure that encapsulates the capsule. When chemical and mechanical stimuli are present, the jellyfish is shot toward the target by the osmotic pressure according to the difference of the calcium concentration. And a series of processes in which the toxin is released from the inside of the jellyfish are produced. The jellyfish extract and the camphor tree extract have an activity of suppressing the release of these jellyfish, respectively, and can act as a deterrent for preventing jellyfish shot. In addition, when the mixture of anthocyanin extract and camphor tree extract were used, the activity of suppressing the release of jaws was improved and the activity was increased when they were mixed with lanthanum.

The present invention provides a composition for combating jellyfish according to the present invention, which is characterized in that the composition comprises an extract of an aquarium of the present invention and / or a camphor extract as an active ingredient.

As the active ingredient of the present invention, the above-mentioned acanthopanax koreanum ) is a deciduous broad-leaved shrub belonging to Araliaceae such as ginseng and is a medicinal plant native to Jeju. Acanthopanax spp. Has been known to have been used as a medicinal herb for the treatment of tongue and neuralgia, rheumatism, hypertension, stroke, diabetes and eczema.

As another active ingredient of the present invention, the camphor tree ( Cinnamomum camphora ) grows mainly in Jeju Island in Korea, and is also called as a tree or oak tree. It is an evergreen broad-leaved arboreous tree with cambicula, 20 m high, 2 m long and has no pests, has good growth and long life span. The fragrance ingredients contained in camphor are essential oil components such as camphor, safflower, and cinere, and contain about 1% of camphor wood, leaf, and fruit. Essential oil is obtained by distilling tree trunks and steam distillation. The essential oil thus obtained is called "camphor" and is used widely as a stimulant or a cardiotonic agent for the treatment of nervous breakdown, epilepsy, cystitis, and pyelonephritis in the private sector. Especially in Japan, camphor is considered very valuable and treated like national ginseng as a national monopoly, and camphor tree has been used as a cancer remedy in the private sector in Jeju Island.

As used herein, the term "extract" refers to the extracts of roots, stems, leaves, flowers, fruits and / or seeds or roots, leaves, flowers, fruits and / or seeds of camphor And more preferably, it can be obtained by using the roots and stem parts of the Japanese apricot and the leaf parts of camphor tree. The extract can be obtained by subjecting the extract to water, anhydrous or hydrous lower alcohol (eg, methanol, ethanol, propanol, butanol, n-propanol, iso-propanol and n-butanol) having 1-4 carbon atoms, acetone, ethyl acetate, The extract is obtained by extracting with chloroform, 1,3-butylenecyclohexane, hexane, diethyl ether or a mixed solvent thereof and an extract obtained by fractionation of the above-mentioned specks in the extract. As long as it is extracted through the step of immersing the object to be extracted in the extraction solvent, it is understood that any of extraction methods such as cold beating, refluxing, heating, and ultrasonic wave can be applied. Nevertheless, the extract is preferably obtained by extracting the extraction object with water, ethanol or a mixed solvent thereof, and includes a concentrated liquid extract or a solid extract from which the extraction solvent has been removed.

As used herein, the term "active ingredient" alone means an ingredient which exhibits the desired activity or which can exhibit activity together with a carrier which itself is not active.

The composition of the present invention, which is an effective component of the extract, may contain any amount (effective amount), depending on the purpose of use, formulation, and compounding purpose, An effective amount will be determined within the range of 0.0001 wt% to 99.9000 wt% based on the total weight of the composition. Here, "effective amount" refers to the amount of effective ingredient capable of inducing the jellyfish fighting effect. Such effective amounts can be determined experimentally within the ordinary skill of those skilled in the art.

The composition for combating jellyfish according to the present invention may further comprise metal cations in addition to an extract from the sea ankle and / or a camphor tree extract.

Since the activity of the divalent cationic calcium-concentration-dependent activity is important, the activity of inhibiting the release of the jellyfish gum in the cationic material to disturb it has been continuously studied (Santorio and Salleo, Experientia 1991, 47, 701-703; Biol. 1993, 184 (1) 97-104; Morabito et al., Cell Physiol Biochem 2014, 34: 244 European Patent No. 02380577; European Patent No. 00984776). Magnesium, Mg, sodium, zinc, zinc, iron, Fe, and the like, as well as lanthanum, La, Gallium, Ga, The activity of various metal cationic substances such as gadolinium (Gd), potassium (K), titanium, Ti, cobalt and Co is known and added to the composition for combating jellyfish of the present invention Can be used. As can be seen from the examples and experimental examples of the present invention, when the extract was used in combination with the lanthanum extract, . Lanthanum (La) is a rare earth element belonging to the lanthanum family and has an oxidation number of 3. The lanthanum can be used in the form of a salt capable of exhibiting cationic activity, and its specific form is not limited.

The term "for jellyfish eradication" of the present invention can be understood as an application for preventing or preventing stings of zebrafish animals represented by jellyfish. The zebrafish animal has an organelle called zupo, and when a certain stimulus is applied, a barb attached to the end of the thread-like structure passes through the target, where the toxin is released. This process is called nematocyst discharge and it is possible to prevent jellyfish from shooting when the process is inhibited.

The composition for combating jellyfish of the present invention can be identified as a cosmetic composition in a specific embodiment.

The cosmetic composition of the present invention may contain, in addition to the active ingredients thereof, conventional additives such as stabilizers, solubilizers, surfactants, vitamins, colorants and antioxidants, and carriers commonly used in cosmetic compositions .

The cosmetic composition of the present invention can be prepared into any of the formulations conventionally produced in the art and can be used as a solution, a suspension, an emulsion, a paste, a gel, a cream, a lotion, a powder, a soap, , Oil, powder foundation, emulsion foundation, wax foundation and spray, but is not limited thereto. More specifically, it can be prepared in the form of a sun cream, a body oil, a flexible lotion, a nutritional lotion, a nutritional cream, a massage cream, an essence, an eye cream, a cleansing cream, a cleansing foam, a cleansing water, a pack, a spray or a powder.

The cosmetic composition of the present invention may further include a component having an ultraviolet blocking effect, which can be understood as a substance having an effect of absorbing ultraviolet rays applied to the skin or inhibiting skin irritation caused by ultraviolet rays. For example, octylmethoxycinnamate (OMC), diethylhexylbutamidotriazone, butylmethoxydibenzoylmethane (avobenzone), ethylhexyltriazone, or titanium dioxide may be used, but are not limited thereto no.

When the formulation of the present invention is a paste, cream or gel, an animal oil, vegetable oil, wax, paraffin, starch, tracant, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide may be used as the carrier component .

In the case where the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component. Especially, in the case of a spray, a mixture of chlorofluorohydrocarbons, propane / Propane, < / RTI > butane or dimethyl ether.

When the formulation of the present invention is a solution or an emulsion, a solvent, a dissolving agent or an emulsifying agent is used as a carrier component. Specifically, water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, 1,3-butyl glycol oil, glycerol aliphatic ester, polyethylene glycol or fatty acid esters of sorbitan.

In the case where the formulation of the present invention is a suspension, a carrier such as water, a liquid diluent such as ethanol or propylene glycol, a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, Cellulose, aluminum metahydroxide, bentonite, agar or tracant, etc. may be used.

When the formulation of the present invention is an interfacial active agent-containing cleansing, the carrier component is selected from aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyltaurate, sarcosinate, fatty acid amide Ether sulfates, alkylamidobetaines, aliphatic alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, lanolin derivatives or ethoxylated glycerol fatty acid esters.

The cosmetic composition of the present invention can be prepared according to the production method of a cosmetic composition known in the art, including its effective component and the above-mentioned carrier component.

The composition of the present invention can be identified as a pharmaceutical composition in another specific embodiment.

The pharmaceutical composition of the present invention may contain, in addition to its active ingredient, a pharmaceutically acceptable carrier, an excipient, and the like. According to a preferred embodiment of the present invention, the pharmaceutical composition of the present invention has an external preparation for skin. (For example, a patch, a bandage, etc.), and the external preparation for skin is not particularly limited. For example, the external preparation may be formulated in the form of a cream, a lotion, an ointment (semi-solid external preparation), a microemulsion, a gel, No.

The term "pharmaceutically acceptable" as used herein means that the application (prescribing) subject does not have the above-mentioned toxicity that is adaptable without inhibiting the activity of the active ingredient.

Examples of pharmaceutically acceptable carriers include lactose, glucose, sucrose, starch (e.g. corn starch, potato starch, etc.), cellulose, derivatives thereof (e.g. sodium carboxymethylcellulose, ethylcellulose, etc.), malt, gelatin, talc, (E.g., peanut oil, cottonseed oil, sesame oil, olive oil, etc.), polyols (e.g., propylene glycol, glycerin and the like), alginic acid, and the like. Depending on the formulation of the pharmaceutical composition of the present invention, one or more suitable ones may be selected and used. Suitable pharmaceutically acceptable carriers and formulations are described in Remington's Pharmaceutical Sciences (19th ed., 1995). The pharmaceutical composition of the present invention may further comprise an emulsifier (e.g., TWEENS), a wetting agent (e.g., sodium lauryl sulfate), a coloring agent, a flavoring agent, a stabilizer, a preservative, water, saline, a phosphate buffer solution and the like.

The excipient may be selected according to the formulation of the pharmaceutical composition of the present invention. For example, suspending agents such as sodium carboxymethyl cellulose, methyl cellulose, hydropropyl methyl cellulose, sodium alginate, polyvinyl pyrrolidone, .

Terms that are not specifically defined in this specification are intended to have a Korean national meaning or a meaning commonly used in the art.

INDUSTRIAL APPLICABILITY As described above, the present invention can provide a composition for combating jellyfish using an islet extract and / or a camphor tree extract.

The composition for combating jellyfish of the present invention can be made into a product such as a cosmetic product or a medicine.

[Figure 1] is an experiment for measuring the activity of suppressing the release of gum from the jellyfish jellyfish.

Hereinafter, the present invention will be described with reference to Examples and Experimental Examples. However, the scope of the present invention is not limited to these examples and experimental examples.

< Example > Sample Preparation

After washing the islets and camphor trees collected from Jeju Island, they were extracted with 70% ethanol solvent and lyophilized. Stem and root parts were used for the aphid, and camphor leaves were used. Lantanium was purchased from LaCl ₃ (Sigma, USA).

In the toxin emission test, the gill jellyfish, which is mainly used as an animal model, was collected from the coastal waters of Jeju Island and the temperature of seawater was maintained between 20 ℃ and 22 ℃. In addition, the experiment was performed on active jellyfish within 48 hours after collection.

< Experimental Example > Experiment to confirm the suppression activity of jellyfish

The jellyfish emits jellyfish as a target when there is a chemical or mechanical stimulation. At the same time, the jellyfish emits toxins inside the jellyfish, causing damage to the jellyfish.

Gelatin coated nylon juloprobe was prepared and used to confirm jellyfish shot inhibition activity through suppression of nasal discharge. Immerse 1 mm thick nylon string in gelatin diluted in purified water to 2 cm length with 30% (w / v). At this time, the thickness of the gelatin of the nylon string is about 0.06 mm and it is solidified by placing it in a humid chamber at 4 ° C for 24 hours.

In order to confirm the activity of suppressing the excretion of jelly from the oak, camphor and lanthanum, the gel was coated at the same concentration as in <Table 1> to prepare a probe. The unit is% (w / v).

division Islet extract Camphor tree extract Lantium Comparative Example - - - Example 1 0.03 - - Example 2 - 0.03 - Example 3 0.015 0.015 - Example 4 - - 0.03 Example 5 0.005 0.005 0.02

After preparing the probe, carefully repeat the process of gently rubbing the oral arm of the jellyfish for 1-2 seconds. Measure the jellyfish discharge. When the jellyfish tentacular cnidocyte is subjected to chemical and mechanical stimulation, a part of the nematocyst twisted in an actual shape penetrates through the target to penetrate the toxin. After the mechanical stimulation as in the present experiment, Can be observed. To this end, the stimulated probe was placed in a well of a microtiter plate containing 50 μl of 1% Trizyme (a mixture of protease and surfactant, Amway Producs, USA) and incubated at room temperature for 4 hours After the incubation, the probes were removed and the number of zippers was confirmed by a 400x optical microscope.

Each experiment group was repeated 4-5 times and the average value of the results was shown in FIG.

As can be seen from the results of FIG. 1, it can be confirmed that the treatment of the island oak or camphor extract suppressed the release of gills compared to the comparative group. In addition, when the extracts were treated with the extracts of Opuntia ficus - indica and camphor tree extracts, the inhibitory effect was more enhanced than that of the extracts treated with La3 + alone.

< Manufacturing example > jellyfish Destructive agent  Manufacturing method

From the above results, the activity of suppressing the release of jellyfish jelly was confirmed, and a jellyfish remover was manufactured using the same.

ingredient Content (wt%) Islet extract 0.5 Camphor tree extract 0.5 LaCl3 1.0 OMC 1.5 glycerin 4 Butylene glycol 4 Hyaluronic acid 5 Beta Glucan 7 Carbomer 0.1 Caprylic capric triglyceride 8 Squalene 0.5 Stearyl glucoside 1.5 Sorbitan stearate 0.4 Stearyl alcohol 1.0 Purified water Up to 100.0

Claims (5)

Wherein the composition comprises at least one member selected from the group consisting of an extract of an aquatic plant extract and a camphor tree extract as an active ingredient and a lanthanum as a metal cation.
The method according to claim 1,
Wherein the composition further comprises at least one metal cation selected from the group consisting of gallium, calcium, magnesium, sodium, zinc, iron, gadolinium, potassium, titanium and cobalt.
The method according to claim 1,
Wherein the composition comprises both an extract of aquarium fish and an extract of a camphor tree.
4. The method according to any one of claims 1 to 3,
Wherein the composition is a cosmetic composition.
4. The method according to any one of claims 1 to 3,
Wherein the composition is a pharmaceutical composition.

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