KR101459707B1 - liquid manure containing quercetin of onion and manufacturing method thereof and production method of functional rice - Google Patents

Abstract

The present invention relates to liquid manure containing quercetin from an onion, a method for producing the same, and a method for producing functional rice using the same. More specifically, the present invention relates to liquid manure, a method for producing the same, and a method for producing functional rice using the same, wherein the liquid manure can produce functional rice containing quercetin which is a kind of flavonoid using liquid manure in which onions are fermented. The liquid manure containing quercetin of the present invention contains the quercetin component of an onion. The method of the present invention comprises: a mixing step of mixing an onion with sugar; and a fermenting step of inoculating and fermenting a strain with the mixture mixed with an onion and sugar.

Description

TECHNICAL FIELD The present invention relates to a quercetin-containing liquid of onion, a method of producing the quercetin, a method of producing the quercetin,

The present invention relates to a liquor containing quercetin of onion, a method of producing the same, and a method of producing functional rice using the same, and more particularly, to a method of producing functional rice containing quercetin, which is a type of flavonoid, And a method for producing functional rice using the same.

Rice has been the ancestor of our ancestors and has formed the food culture of our country as the basis of our eating habits. In the past, when rice self-sufficiency was difficult, the increase of rice was an important task of agriculture, and efforts have been made to increase rice cultivation policies such as development of large variety varieties and development of cultivation techniques.

At the same time, in order to supplement deficient rice, wheat grain and marriage were strongly encouraged at once. Besides lack of nutritional research on rice in addition to seasoning exercise, wheat nutrition was once emphasized.

Therefore, the correct information on the nutrition and health of rice has not been provided, and misperception about rice has spread. Since then, rice has been self-sufficient since the 1980s due to the continuous promotion of rice and the development of breeding and cultivation technologies. Since then, the production of rice has continued to increase.

However, compared with the increasing supply of rice, the consumption of rice has been rapidly declining as our diet has been westernized recently. Therefore, the decrease in the consumption of rice caused the decrease of the rice price, and the farmers suffered a great difficulty. In addition, due to the considerable economic loss of domestic farmers and related companies due to the opening of the international rice market, the necessity of institutionalization for rice consumption measures, It is necessary to develop a new technology that can be differentiated.

In response to these demands, the development of functional rice has recently emerged. The emergence of such functional rice has changed the concept of rice as a stock from quantity to quality, .

In recent years, functional rice has been developed which has various flavors and nutrients in rice. For example, functional rice coated with ginseng, chitosan, germanium, mushroom, kelp, etc. has been developed recently . Korean Patent No. 10-0574761 discloses a functional rice added with polyphenols, minerals and vitamins.

However, such conventional functional rice is obtained by coating the functional materials on the surface of the rice, and there is a problem in that the sensual taste such as taste and aroma does not reach the consumers' preference, and the problem that the functional materials are lost in the washing process of rice have. In addition, there is a problem that a separate coating facility for the production of functional rice must be provided, and therefore a separate coating operation must be carried out after the rinsing, which is uneconomical.

The present invention has been made in order to overcome the above problems, and it is an object of the present invention to provide a liquefied petroleum liquefied petroleum liquefied petroleum gas containing liquefied petroleum jelly, which is not simply coated with the quercetin component derived from onion on the surface of rice, And a method for producing functional rice using the same.

In order to achieve the above object, the quercetin-containing solution of the present invention is characterized by containing a quercetin component of onion.

In order to accomplish the above object, the present invention provides a method of preparing a quercetin-containing solution, comprising: mixing an onion and a sugar; And a fermentation step of inoculating the mixture with the mixture of onion and sugar and fermenting the mixture.

The strain is characterized by being Bacillus subtilis.

The fermentation step is characterized in that the mixture is fermented by adding a spirit to the mixture.

In order to accomplish the above object, the present invention provides a method for producing quercetin-containing functional rice, wherein the liquid is sprayed on growing rice to produce rice containing quercetin.

The liquid fertilizer is sprayed 2 to 6 times and is sprayed with 450 to 1500 liters per 1500 square pounds per spraying to cultivate the rice.

As described above, according to the present invention, an onion may be fermented to produce a juice containing a quercetin.

These liquids can be applied to growing rice to produce functional rice containing quercetin. In addition, the fertilization of the livestock can promote the growth of rice. Therefore, it is possible to develop high-performance rice which can contribute to the increase of rice production and the added value, and satisfy the demand of the consumer.

1 is a graph showing the quercetin content of the liquid prepared according to Examples 1 to 4,
Fig. 2 is a graph showing the results of quercetin content of pots produced according to Examples 5 to 8,
3 is a graph showing the growth rate of rice grown in a laboratory,
Figs. 4 to 6 are graphs showing the results of quercetin content for rice, brown rice, 7 minutes, 9 minutes, and 11 minutes.

Hereinafter, a quercetin-containing liquid of onion according to a preferred embodiment of the present invention, a method for producing the same, and a method for producing functional rice using the same will be described in detail.

The liquid fertilizer according to an embodiment of the present invention is characterized by containing a quercetin component extracted from an onion.

The main physiologically active substances contained in onion are organic sulfur compounds and flavonoid compounds. Flavonoids are non-enzymatic bio-pigments containing anthocyanins and antacoxanthin. Flavonoids are polyphenolic compounds that are widely distributed in plants and vegetables such as licorice and bank.

Quercetin (quercetin) is a yellow-type flavonoid which has a high content of antioxidant activity and is effective in inhibiting oxidative damage of cells. In particular, onions contain a large amount of quercetin, which is known to have a high content of colored shells.

The liquid mixture of the present invention may contain 0.1 to 1.2 mu g / ml of quercetin as an example.

A mixing step of mixing the onion and the sugar by the method for preparing the sauce; and a fermentation step of inoculating the mixture with the mixture of the onion and the sugar to ferment.

First, the onion and sugar are mixed in the mixing step. For example, a certain amount of water may be poured into a fermentation vessel, sugar may be added to the fermentation vessel to dissolve it in water, and the onion may be added to the fermentation vessel to be mixed. For example, 500 to 1500 parts by weight of water and 20 to 80 parts by weight of water per 100 parts by weight of onion.

The onion, which is the main material for making the livestock, contains the onion's skin. Onions can be used in crushed or crushed form crushed with a chopped piece or blender.

Sugar or molasses can be used as a sugar or a mixture thereof. It is needless to say that various additives may be added in addition to the sugar. The additive may be added in an amount of 10 to 50 parts by weight based on 100 parts by weight of the onion.

Examples of additives include agricultural by-products, livestock by-products, fish by-products, minerals, vinegar, charcoal, enzymes and the like.

Examples of agricultural byproducts are herbals such as Chinese medicine, Chinese herbal medicine, herbs, fruits, vegetables, rice bran, soybean meal, and livestock byproducts such as bovine, swine, fowl, blood, bone meal, (Seaweed), phosphorus, potassium, potassium hydroxide, sodium bicarbonate and the like can be cited as the minerals. , Enzymes such as chitase, chymotrypsin, and calfain.

Next, the strain is inoculated into a mixture of an onion and a sugar to ferment. Preferably, the mixture may be fermented by addition of a spirit. Fermentation with the addition of alcohol can increase the extraction effect of quercetin during the fermentation process. Ethanol in a concentration of 10 to 30% can be used as the alcohol.

As a strain for fermentation, Bacillus subtilis subtilis), a brush as Bacillus stearate Russ (Bacillus stearothermophilus), also Pseudomonas Manas (Rhodopsudomonas), also RY rilrum (Rhodospirillum), Bacillus Sono alkylene sheath (Bacillus sonorensis , Bacillus thermoamylovorans), Saccharomyces as Mrs. Lucy (the Saccharomyces rouxii), Mrs. Celebi Asia as Saccharomyces (Saccharomyces cereviciae), Miss Mrs. Paul Ob as Saccharomyces (Saccharomyces oviformis), MRS stay Neri as Saccharomyces (Saccharomyces steineri ), Aspergillus oryzae , Aspergillus niger can be used together or two or more together.

Preferably, Bacillus subtilis is used as a strain. Bacillus subtilis is a microorganism well known as a fermentation strain of Chungkookjang or Meju, and produces antibiotics and antioxidants, which makes it suitable for the production of the slurry of the present invention.

The above-mentioned strain may be used in the form of a culture liquid cultured in a conventional medium, or may be obtained by freeze-drying and commercial products commercially available on the market.

After the strain is inoculated, it is stored at a temperature of 20 to 30 ° C for about 5 to 10 days and fermented to produce a liquid pot.

One example of a method for producing the quercetin-containing functional rice using the thus-prepared liquid fertilizer is to spray rice bran under growing to grow rice.

The rice cultivation method can be applied to the conventional method of growing the seedlings or the direct seeding method. It is a method of cultivating rice seeds by germinating rice seeds in a mating area and transferring them to an answer in a certain manner. Most of the rice cultivation methods in this country are rice cultivation.

Direct seed cultivation is a method of seeding seeds directly into the seedlings without seedling seeds and allowing the seeds to grow until they are harvested. The seeds can be divided into dry seed cultivation method and direct water direct cultivation method according to water management. Spraying of the liquid is done during the growth of the rice in this answer. It is preferable to apply the leaf surface spraying method during the growing period of rice. Liquor can be added to the irrigation water or sprayed directly on the soil, but leafing is effective. Foliar spraying is beneficial in reducing the change in physico - chemical properties of the soil and allowing quercetin to be absorbed by long - growing crops such as rice.

Liquor is sprayed 2 to 6 times during the growth period of rice, and 450 to 1,500 liters can be sprayed at 1,500 pyeong per spraying. When the number of application times or the application amount is less than the above range, the content of quercetin contained in rice is low, and when the number of application times or application amount exceeds the above range, the improvement of the content is not high and it is inefficient.

As described above, quercetin can be contained in the harvested rice by spraying the rice bran containing the quercetin in the growing rice and cultivating the rice. The rice produced by the rice production method of the present invention has a very high quercetin content of 4 to 50 占 퐂 / g. In particular, the content of quercetin is significantly increased in brown rice compared to white rice. The rice produced by the rice production method of the present invention is preferably ingested in the form of brown rice.

Hereinafter, the present invention will be described in more detail with reference to the following examples. However, the scope of the present invention is not limited by these examples.

(Experimental Example)

1. Liquid experiment

(1) Manufacture of pots

100 liters of water was poured into a fermentation tank equipped with an aeration device, and 20 kg of molasses was added, followed by stirring to dissolve the molasses. Then, 50kg of onion produced in Muan-gun, Jeollanam-do was put into a fermentation tank, and 50kg of fermentation tank was put into a fermentation tank. Then, 1L of Bacillus subtilis culture and 400L of water were put into a fermentation tank and mixed evenly. Thereafter, the fermentation tank was fermented for 1 to 7 days while keeping the inside of the fermentation tank at a temperature of 25 ° C to prepare a liquid ratio. During the fermentation period, the fermentation was carried out twice a day by aerating the air into the fermentation tank through the aeration device.

Bacillus subtilis (KCTC 18170P) was cultured for 2 days at 35 ° C in a medium consisting of 3 g of Tryptone, 3 g of yeast extract, 3 g of glucose, 5 g of NaCl and 1 g of K ₂ HPO _{4 in} 1 L of distilled water. TSB (Tryptic Soy Broth) medium and incubated at 35 DEG C for 96 hours.

Fermentation was carried out by varying fermentation period of 5 days and 7 days in order to compare the contents of quercetin with the addition of fermentation period. The fermentation was carried out with and without the addition of alcohol, and when the fermented tuna was added to the fermentation tank, 20 liters of the fermented liquor was added with the onion.

The fermentation period of fermentation period was divided into Examples 1 to 12, and the fermentation period was summarized in Table 1 below.

division Whether alcohol is added Fermentation period Example 1 No additives 5 days Example 2 Addition (10% ethanol) 5 days Example 3 Addition (20% ethanol) 5 days Example 4 Addition (30% ethanol) 5 days Example 5 No additives 7 days Example 6 Addition (10% ethanol) 7 days Example 7 Addition (20% ethanol) 7 days Example 8 Addition (30% ethanol) 7 days

(2) Experimental method

After the sample was pretreated, the amount of quercetin contained in the liquid rice and rice was measured using LC-MS.

Pretreatment of samples for LC-MS analysis was performed by adding 40 mL of 50% methanol to 2 g of the sample and treating the mixture in an ultrasonic mill for 15 minutes. After the extraction of the supernatant, the supernatant was centrifuged to remove the supernatant. After the extract was poured into a volume of 100 mL, it was purified using a sep-pak C18 cartridge and the sample was finally extracted. The final extract was concentrated by using a nitrogen concentrator, dissolved in acetonitrile, and filtered to be used as an analytical sample.

Condition of HPLC conditions are shown in Table 2 below.

Company Agilent 1100 series (Binary pump systems) Column Symmetry R C18 3.5 m 2.1 x 10 mm (Waters) μ Mobile Phase A: 5% acetic acid in ACN B: 5% acetic acid in Water


Gradient


Time  A (%) B (%) Flow 0 min 5 95 0.4 mL / min 30min 40 60 0.4 mL / min 31min 80 20 0.4 mL / min 40min 80 20 0.4 mL / min 41 min 5 95 0.4 mL / min 45min 5 95 0.4 mL / min

And Condition of Mass Spectrometer conditions are shown in Table 3 below.

Company Bruker Esquire HCT (Ion Trap MS) Ion source ESI Mass Range 100-700 m / z Polarity Dry Negative Nebulizer gas 40 psi Gas Dry Gas 9 L / min Scan Mode Ultrascan Temp 365 ° C Capillary voltage -4000V

(3) Experimental results

The results of measuring the quercetin content of Examples 1 to 8 are shown in Figs. 1 and 2. Fig. FIG. 1 shows the results of Examples 1 to 4 in which the fermentation period is 5 days, and FIG. 2 shows the results of Examples 5 to 8 in which the fermentation period is 7 days.

Referring to FIGS. 1 and 2, as a result of the measurement, the content of quercetin increased as the fermentation period passed. The content of quercetin in the fermented juice was significantly higher than that of the fermented juice. Especially, when 20% ethanol was added, the content of quercetin was the highest, which was higher than that of 30% ethanol.

Therefore, fermentation for 5 to 7 days with the addition of alcohol seems to be advantageous in terms of the content of quercetin.

2. Rice cultivation experiment

(1) Laboratory experiments

In the nutrient medium (control group) in the laboratory, when the liquid of Example 5 was added to the nutrient medium (experimental group 1), when the liquid of Example 7 was added to the nutrient medium (experimental group 2) And the growth rate (height cm) was measured. Hyayami was used as a rice variety. The measurement results are shown in Fig.

Referring to FIG. 3, the experimental results showed that the growth rates of experimental groups 1 and 2 were the best. Therefore, when the liquid fertilizer of the present invention is used, it is expected that not only rice containing quercetin can be produced but also the growth of rice is promoted.

(2) Packing test

The content of quercetin in the harvested rice was investigated by application of the liquid in actual packaging. The packaging test was conducted from July 2012 to September 2012. Liquid fertilizer was diluted with water (liquid ratio: water = 1: 10 weight ratio), sprayed on the leaf surface using a broad range spreader, and sprayed with the liquid fertilizer with different application times and one dose. Lactic acid produced under the conditions of the above Example 7 was used.

The content of quercetin was investigated in rice bran, brown rice, and white rice grown for 7 minutes, white rice for 9 minutes and white rice for 11 minutes. The prepared samples were subjected to sample pretreatment and analyzed by LC-MS as described above.

division Number of Sprays
Spray interval Spray amount per one time
Onion 1 group

3rd time
1st: 7/30 ~ 8/1
2 weeks

450ℓ / 1500㎡
Second: 8/13 - 8/15 Third: 8/27 - 8/29
Onion 2 groups

3rd time
1st: 7/30 ~ 8/1
2 weeks

1500ℓ / 1500㎡
Second: 8/13 - 8/15 Third: 8/27 - 8/29

Onion 3 groups



6 times


1st: 7/30 ~ 8/1

1 week



1500ℓ / 1500㎡

Second: 8/6 ~ 8/8 Third: 8/13 - 8/15 4th: 8/20 ~ 8/22 5th: 8/27 ~ 8/29 6th: 9/3 ~ 9/5

The experimental results are summarized in Table 5 below. The results for brown rice, 7 minutes, 9 minutes, and 11 minutes except for rice bran are shown in Figs. 4 to 6.

division  group Quercetin content (ug / g)
Rice bran
Onion 1 group 293.67 Onion 2 groups 789.58 Onion 3 groups 162.93
Brown rice
Onion 1 group 50.53 Onion 2 groups 22.50 Onion 3 groups 32.27
Seven minutes
Onion 1 group 27.58 Onion 2 groups 11.77 Onion 3 groups 38.92
9 minutes
Onion 1 group 12.30 Onion 2 groups 7.80 Onion 3 groups 4.63
11 minutes
Onion 1 group 16.40 Onion 2 groups 16.55 Onion 3 groups 6.64

As a result of the experiment, it was confirmed that quercetin was contained in the rice harvested when the liquid containing quercetin was sprayed during the growth period of rice. Overall, the quercetin content of brown rice was higher than those of 7, 9, and 11 minutes of rice. In addition, quercetin content gradually decreased in the 7th, 9th, and 11th minutes. In some cases, however, different patterns were observed at 7 minutes, 9 minutes and 11 minutes, presumably due to cross contamination during the rice process.

In comparison between the onion 2 group and the onion 3 group, the relation between the number of liquid application and quercetin content was higher than that of 6 times in the brown rice and 7 minutes in the case of 3 times in the onion, but 6 times in 9 minutes and 11 minutes, Respectively. Therefore, it can be seen that the quercetin content does not increase proportionally with the number of spraying times.

And comparing the onion 1 group with the onion 2 group, the relationship between the amount of squash and the amount of squash was examined. When the amount of rice was applied, rice bark, which is rice husk, had more quercetin. However, in case of rice 7, 9, 11, . These results suggest that an appropriate amount of spraying may help increase the quercetin content of rice.

While the present invention has been described with reference to exemplary embodiments, it is to be understood that the invention is not limited to the disclosed exemplary embodiments. Accordingly, the true scope of protection of the present invention should be determined only by the appended claims.

Claims (6)

A quercetin-containing sauce comprising an onion quercetin component. A mixing step of mixing onion and sugar;
And a fermentation step of inoculating a fermented mixture with the mixture of onion and sugar to ferment the fermented product. 3. The method according to claim 2, wherein the strain is Bacillus subtilis. 3. The method according to claim 2, wherein the fermentation step comprises fermenting the mixture by adding a spirit to the mixture. A method for producing a quercetin-containing functional rice, characterized in that the liquid of claim 1 is sprayed on growing rice to produce rice containing quercetin. [Claim 6] The method according to claim 5, wherein the liquid fertilizer is sprayed 2 to 6 times, and 450 to 1500 liters are sprayed at 1500 pyeong per spraying to cultivate the rice.

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