KR101458344B1 - Preparation method of ginseng hydrolysate concentrate or powder having high concentration of ih-901 - Google Patents

Abstract

The present invention relates to a method for producing a ginseng hydrolysate concentrate or a ginseng hydrolysate powder containing high concentration IH-901 by adding enzymes in a ginseng extract or a ginseng liquid in order to be hydrolyzed. Disclosed are: a method for producing a ginseng hydrolysate concentrate or a ginseng hydrolysate powder with enzyme combination and improved processes by which an IH-901 content can be extremely improved; and a functional food composition using the same. The present invention provides a method for producing a ginseng hydrolysate concentrate and a functional food composition using the same, wherein the method comprises the steps of: (a1) producing a ginseng extract by applying water or an organic solvent to ginseng; (b1) cooling and dipping the ginseng extracted produced from the (a1) step; mixing pectinase and cellulose with the supernatant of the ginseng extract dipped through the (b1) step in order to be hydrolyzed; and (d1) concentrating the ginseng extract hydrolyzed from the (c1) step.

Description

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a method for producing a ginseng hydrolyzed concentrate or a ginseng hydrolyzed powder containing a high concentration of IH-

More particularly, the present invention relates to a method for producing a ginseng hydrolyzed concentrate or a ginseng hydrolyzed powder, and more particularly, to a method for producing a ginseng hydrolyzed concentrate or a ginseng hydrolyzed powder containing a large amount of IH- To a method for producing decomposition powder.

Ginseng (Panax ginseng C.A. Meyer. Araliaceae) is one of the medicines traditionally used for the treatment of various diseases in Asian countries such as China, Korea, and Japan. In particular, ginsenoside (saponin), which is a major active ingredient of ginseng, has antioxidant activity, anti-inflammatory activity, antioxidant activity in the central nervous system and cardiovascular system and immune system (see Non-Patent Documents 1 to 3) (See Document 4) and antitumor activity (see Non-Patent Document 5).

On the other hand, it has been known that ginsenosides are metabolized by intestinal microorganisms after ingestion, and their metabolites also have various physiological activities (see Non-Patent Documents 6 to 8). For example, protopanaxadiol-based ginsenosides Rb1, Rb2, and Rc can be detected by intestinal microorganisms in humans, such as IH-901 (20-O- beta -D-glucopyranosyl- (See Non-Patent Documents 9 and 10), Re and Rg1, which are proximal triazine-based ginsenosides, are metabolized by ginsenoside Rh1 (See Non-Patent Documents 11 and 12), the metabolites IH-901, Rh1 and F1 exhibit various physiological activities.

In particular, it is known that IH-901 is mainly antidiabetic (see non-patent document 13) and immuno-enhancing action, and is also known to induce antitumor or anticancer effects by preventing intrusion of the tumor or by preventing chromosome deformation and tumor formation (See non-patent documents 14 and 15).

For this reason, efforts have been extensively made to convert ginsenosides into metabolites. Some researchers have attempted to produce genuine prosapogenin or sapogenin by chemical synthesis, weak hydrolysis, alkaline degradation (see Non-Patent Documents 16 to 18). However, these methods cause various side reactions such as epimerization, hydration, hydroxylation and the like. Recently, enzymes (see non-patent documents 19 and 20) and intestinal bacteria (non-patent document 9 And 21) have been studied in various ways to convert ginsenosides under mild conditions.

In particular, methods using enzymes for producing IH-901 from ginsenosides have been studied. Specifically, a method of producing IH-901 by hydrolyzing a diol-based ginsenoside with a Naringase enzyme, Side was orally administered to rats and then decomposed in the large intestine to produce IH-901. However, this production method has a problem that not only the production yield of IH-901 is extremely low but also various secondary metabolites are produced, thereby producing high-purity IH-901 (see Non-Patent Document 22). Accordingly, a method of producing IH-901 by reacting beta-galactosidase enzyme with ginsenosides Rb1, Rb2, Rc, Rd, or a mixture thereof has been developed (see Patent Document 1), and Nalin Gina and Pectinase (Patent Document 2) was developed by reacting a cellulase or a beta-galactosidase enzyme with purified ginsenoside to prepare IH-901 (Patent Document 2), and a method of reacting a cellulase or a beta-galactosidase enzyme with a ginsenoside Rb1, Rb2, Rc, Rd, To prepare IH-901 (see Patent Document 3). This method is inconvenient for producing ginseng hydrolyzed concentrate containing a large amount of IH-901 because the production method is very complicated because each ginsenoside is separated and used instead of the main ingredient of ginseng concentrate. Therefore, the fermented product produced by this method is difficult to be directly used as a food, and the amount of IH-901 produced is small, so that it is difficult to expect its efficacy.

In order to overcome this problem, the inventors of the present invention have found that when pectinase and beta-galactosidase are mixed under specific conditions and then added to the ginseng extract or ginseng solution to ferment under specific conditions, a fermented ginseng concentrate or fermentation product containing high concentration of IH- It has been disclosed that ginseng powder can be produced (see Patent Document 4). In this patent, a fermented ginseng concentrate or a fermented ginseng powder having an improved IH-901 content has been proposed. However, it is still necessary to develop a manufacturing technology of a fermented ginseng concentrate or fermented ginseng powder having a high concentration of IH- to be.

[Patent Literature]

Patent Document 1: Korean Patent Laid-Open No. 10-2003-0094757 (Dec. 18, 2003)

Patent Document 2: Korean Patent Publication No. 10-0418604 (Feb.

Patent Document 3: Korean Patent Publication No. 10-0377546 (Mar. 26, 2003)

Patent Document 4: Korean Patent Registration No. 10-0954851 (Apr. 28, 2010)

[Non-Patent Document]

Non-Patent Document 1: Wu JY, et al., J. Immunol., 148: 1519-25, 1992.

Non-Patent Document 2: Lee FC., Facts about ginseng, the elixir of life. Hollyn International. New Jersey, 1992.

Non-Patent Document 3: Huang KC., The pharmacology of Chinese herbs. CRC Press. Florida, 1999.

Non-Patent Document 4: Chang HM., Pharmacology and application of Chinese material medica. Vol1. World Scientific. Singapore, 1986.

Non-Patent Document 5: Sato K, et al., Biol. Pharm. Bull. 17: 635-9,1994; Mochizuki M, et al., Biol. Pharm. Bull. 18: 1197-1202,1995.

Non-Patent Document 6: Karikura M, et al., Chem. Pharm. Bull, 39: 2357-61, 1991.

Non-Patent Document 7: Kanaoda M, et al., J. Tradit. Med. 11: 241-5,1994.

Non-Patent Document 8: Akao T, et al., Biol. Pharm. Bull. 21: 245-9,1998.

Non-Patent Document 9: Hasegawa H, et al., Planta Medica 63: 463-40, 1997.

Non-Patent Document 10: Tawab MA, et al., Drug Metab. Dispos. 31: 1065-71, 2003.

Non-patent document 11: Hasegawa H, et al., Planta Medica 62: 453-7, 1996.

Non-Patent Document 12: Tawab MA, et al., Drug Metab. Dispos. 31: 1065-71, 2003.

Non-Patent Document 13: Yun Suk Choi et al., J Ginseng Res. 31 (2): 79-85, 2007.

Non-Patent Document 14: Wakabayashi C, et al., Oncol. Res. 9: 411-7,1998.

Non-Patent Document 15: Lee SJ, et al., Cancer Lett. 144: 39-43,1999.

Non-patent document 16: Han BH, et al., Planta Medica 44: 146-9, 1982.

Non-Patent Document 17: Chen Y, et al., Chem. Pharm. Bull. 35: 1653-5, 1987.

Non-Patent Document 18: Elyakov GB, et al., Synthesis of the ginseng glycosides and their analogs. Proc. 6th Int. Ginseng Symp. Seoul 74-83, 1993.

Non-patent document 19: Ko SR, et al., Biosci. Biotechnol. Biochem. 64: 2739-43,2000.

Non-Patent Document 20: Ko SR, et al., Planta Med. 69: 285-6, 2003.

Non-Patent Document 21: Bae EA, et al., J. Microbial. Biotechnol. 13: 9-14, 2003.

Non-patent reference 22: Karikura et al., Chem. Pharm. Bull. 38: 2859,1990.

SUMMARY OF THE INVENTION Accordingly, it is an object of the present invention to provide a ginseng hydrolyzed concentrate or ginseng hydrolyzed powder containing high concentration of IH-901 by adding an enzyme to an extract of ginseng extract or ginseng, The present invention provides a method for producing a ginseng hydrolyzed concentrate or ginseng hydrolyzed powder having an enzyme combination capable of dramatically improving the content of IH-901 and an improved process, and a functional food composition using the same.

(A1) preparing ginseng extract by adding water or an organic solvent to ginseng; (b1) cooling and immersing the ginseng extract prepared in the step (a1); (c1) adding the pectinase and the cellulase to the supernatant of the ginseng extract immersed in the step (b1), and then hydrolyzing the mixture; And (d1) concentrating the ginseng extract hydrolyzed in the step (c1).

According to another aspect of the present invention, there is provided a method for preparing ginseng, comprising: (a2) preparing ginseng solution by adding water or an organic solvent to ginseng; (b2) adding the pectinase and the cellulase to the ginseng solution prepared in the step (a2), and then hydrolyzing the mixture; (c2) separating the hydrolyzed ginseng solution and the hydrolyzed ginseng in the step (b2); (d2) adding water or an organic solvent to the hydrolyzed ginseng in which the ginseng liquid has been separated in step (c2); (e2) cooling and immersing the ginseng solution separated in the step (c2) and the extract solution extracted in the step (d2) after cooling; And (f2) concentrating the supernatant of the extract solution immersed in the step (e2).

The ginseng in step (a1) or step (a2) may be selected from the group consisting of ginseng, white ginseng, ginseng, dried ginseng, red ginseng, Taekguk ginseng, their extract, powder thereof and combinations thereof. And a manufacturing method thereof.

In the step (c1) or (b2), the content of the pectinase and the amount of the cellulase is 0.1 to 100 parts by weight of the cellulase with respect to 1 part by weight of the pectinase. In the step (a1) Wherein a mixture of the pectinase and the cellulase is added in an amount of 0.1 to 50 parts by weight based on 100 parts by weight of the extracted ginseng extract or ginseng solution.

The hydrolysis of step (c1) or step (b2) is performed at a pH of 3 to 8 and at a temperature of 10 to 70 ° C for 1 to 72 hours.

In the step (b1) or (e2), the cooling is performed at 0 to 10 ° C at room temperature, and the immersion is performed for 1 to 12 hours.

In another aspect of the present invention, the present invention provides a method for producing ginseng hydrolyzed powder by drying the ginseng hydrolyzed concentrate under reduced pressure or freeze-drying.

In order to solve the above-mentioned problems, the present invention provides a functional food composition containing a ginseng hydrolyzed concentrate or ginseng hydrolyzed powder hydrolyzed with a mixed enzyme of pectinase and cellulase in ginseng.

Also, the ginseng hydrolyzed concentrate or ginseng hydrolyzed powder has a content of IH-901 of more than 36 mg / g.

Also, the ginseng hydrolyzed concentrate or ginseng hydrolyzed powder contains ginsenosides Rh2, F1 and F2.

Also, the food composition provides a functional food composition having immunity enhancement, anti-diabetic, anti-cancer, anti-aging, antioxidant and skin wrinkle improving effect.

According to the present invention, by immersing, hydrolyzing and concentrating the ginseng extract or the ginseng solution using a mixed enzyme comprising pectinase and a cellulase, the ginseng hydrolyzed concentrate having a significantly improved IH-901 content Or ginseng hydrolyzed powder, and a functional food composition having immunosuppression, antidiabetic, anti-cancer, anti-aging, antioxidant and skin wrinkle-reducing effects using the method.

Hereinafter, preferred embodiments of the present invention will be described in detail. DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS Hereinafter, the present invention will be described in detail with reference to the accompanying drawings. Throughout the specification, when an element is referred to as "including " an element, it means that it can include other elements, not excluding other elements, unless specifically stated otherwise.

The inventors of the present invention have found that a ginseng hydrolyzed concentrate containing high concentration of IH-901 or a method capable of further improving the content of IH-901 in the production of ginseng hydrolyzed powder through a hydrolysis process using a mixed enzyme to a conventional ginseng concentrate or ginseng solution The present inventors have found that when a combination of pectinase and cellulase is newly developed and used as a mixed enzyme for hydrolysis and a low temperature rapid cooling and immersion process is employed, dramatically improved IH -901 content, but the metabolite which has not been obtained conventionally is additionally expressed, leading to the present invention.

Accordingly, the present invention provides, as an embodiment, (a1) preparing ginseng extract by adding water or an organic solvent to ginseng; (b1) cooling and immersing the ginseng extract prepared in the step (a1); (c1) adding the pectinase and the cellulase to the supernatant of the ginseng extract immersed in the step (b1), and then hydrolyzing the mixture; And (d1) concentrating the ginseng extract hydrolyzed in the step (c1), thereby producing a ginseng hydrolyzed concentrate.

According to another aspect of the present invention, there is provided a method for producing ginseng, comprising the steps of: (a2) adding water or an organic solvent to ginseng to prepare a ginseng solution; (b2) adding the pectinase and the cellulase to the ginseng solution prepared in the step (a2), and then hydrolyzing the mixture; (c2) separating the hydrolyzed ginseng solution and the hydrolyzed ginseng in the step (b2); (d2) adding water or an organic solvent to the hydrolyzed ginseng in which the ginseng liquid has been separated in step (c2); (e2) cooling and immersing the ginseng solution separated in the step (c2) and the extract solution extracted in the step (d2) after cooling; And (f2) concentrating the supernatant of the extract solution immersed in the step (e2), thereby producing a ginseng hydrolyzed concentrate.

In the step (a1) or (a2), the ginseng is not particularly limited, but is preferably selected from the group consisting of Mishima, White ginseng, Ginseng, Dried ginseng, Red ginseng, Taekwi gum, Extracts thereof, And more preferably Mitsamishi, white ginseng, fresh ginseng or ginseng powder may be used.

The organic solvent used for the extraction in the step (a1) or (d2) is not particularly limited, but preferably an organic solvent usable for the production of food can be used alone or in combination, more preferably ethanol ) Can be used.

In the step (a1), the ginseng extract is prepared by adding water or an organic solvent to ginseng to produce an extract of ginseng by a method known to a person skilled in the art. Preferably, water and an organic solvent are mixed and used. The use of a mixture of organic solvents can increase the solubility of the intermediate reactant produced by the enzyme reaction, and ultimately improve the content of IH-901 in the ginseng hydrolyzed concentrate or the ginseng hydrolyzate.

When water and ethanol are mixed, the mixing ratio may be 1: 0.1 to 1:10, preferably 1: 0.5 to 1: 5, more preferably 1: 0.5 to 1: 1.5 . In addition, the ratio of the solvent mixed to the ginseng can be 2 to 10 times, preferably 3 to 7 times, more preferably 4 to 6 times the weight of the total substrate to produce ginseng extract.

The process of extracting hydrolyzed ginseng in step (d2) may also be performed in a similar manner to the extraction process of step (a1).

The step (b1) or (b2) is a step of hydrolyzing a ginseng extract or a ginseng solution with a specific combination of a mixed enzyme. In the present invention, a mixed enzyme consisting of a pectinase and a cellulase is used.

At this time, the composition ratio of the mixed enzyme may be 0.1 to 100 parts by weight of the cellulase based on 1 part by weight of pectinase, and preferably 0.1 to 10 parts by weight of the cellulase for maximizing the content of IH-901 and increasing the content of other ginsenosides And more preferably 0.1 to 5 parts by weight of a cellulase, and most preferably 0.5 to 3 parts by weight of a cellulase.

In addition, the content of the mixed enzyme in the ginseng extract or ginseng solution also influences the content of IH-901 and other ginsenosides, and the mixture of pectinase and cellulase is added to the ginseng extract or ginseng solution in an amount of 0.1 To 50 parts by weight have been found effective to increase the content of IH-901 and other ginsenosides, preferably 0.5 to 40 parts by weight, more preferably 1 to 30 parts by weight .

The hydrolysis process also requires an optimum pH, reaction temperature and time for increasing the IH-901 content and other ginsenoside components. In the present invention, when the hydrolysis is carried out at pH 3 to 8 and 10 to 70 ° C for 1 to 72 hours, it is effective to increase the content of IH-901 and other ginsenosides. In the case of pH, the activity of the pectinase and the cellulase enzyme can be maintained within the above range. In order to maximize the content of IH-901, it is preferable that the pH is 4 to 6, and the pH of 4 to 5 is the most preferable. In order to maximize the content of IH-901, the reaction temperature is preferably 40 to 60 ° C, more preferably 50 to 60 ° C, still more preferably 55 to 60 ° C, Lt; RTI ID = 0.0 > 60 C < / RTI > In order to maximize the content of IH-901, the reaction time is preferably 10 to 60 hours, more preferably 10 to 24 hours, more preferably 15 to 24 hours, 20 hours was most preferable.

In the present invention, when pectinase and cellulase are separately added, as well as when conventional pectinase and beta-galactosidase are mixed, when the pectinase and cellulase are mixed, the final ginseng hydrolyzed concentrate or The inventors of the present invention found that when the pectinase and the cellulase are added to the ginseng extract or the ginseng solution in the above-mentioned amounts, the ginseng hydrolyzate and the ginseng hydrolyzate Or IH-901 content of the hydrolyzed powder of ginseng can be maximized. The reason why the content of IH-901 is greatly improved when pectinase and cellulase are mixed is higher than that of the case where the conventional pectinase and beta-galactosidase are mixed, but the reason why the cellulase component is Rb1, Rb2, It is believed that the metabolism of Rc and Rd improves the metabolism of IH-901, simplifies the pathway for producing IH-901 through various pathways, and metabolizes each ginsenoside.

The step (b1) or (e2) is a step of immersing the hydrolyzed ginseng extract or hydrolyzed extract at a low temperature, thereby increasing the removal efficiency of impurities that may affect the enzyme reaction, thereby inhibiting the reaction by impurities Thereby maximizing the content of IH-901 in the final ginseng hydrolyzed concentrate or ginseng hydrolyzed powder, as well as allowing other ginsenoside components to be produced at a high yield.

The low temperature immersion may be carried out by rapidly cooling the hydrolyzed ginseng extract or hydrolyzed extract and immersing it in a rapid cooling process at room temperature to 10 ° C or less, preferably 0 to 10 ° C Preferably 1 to 5 占 폚, most preferably 2 to 4 占 폚, with a rate of 1 to 10 占 폚 / min, preferably 3 to 7 占 폚 / min.

The immersing method is not particularly limited. As the solvent used for the immersion, at least one solvent may be selected from the group consisting of solvents usable for food production, preferably water, alcohol (ethanol) or a mixture of selected solvents Can be used. More preferably, no solvent may be used. The immersion time is not particularly limited, and can be, for example, immersed for 1 to 12 hours.

The step (d1) or the step (f2) is a step of concentrating the ginseng extract or the extract immersed in the step (c1) or (e2) to prepare a final ginseng hydrolyzed concentrate. But it can be carried out by concentrating only the supernatant of the immersed ginseng extract or the extract and concentrating it under reduced pressure at 40 to 100 ° C, preferably 60 to 90 ° C, using a commonly used vacuum decompression concentrator.

The ginseng hydrolyzed powder according to the present invention can be prepared by vacuum drying or lyophilizing the ginseng hydrolyzed concentrate prepared as described above. And can be carried out using a hot-air drier or a freeze drier which is conventionally used for vacuum drying or freeze-drying.

According to the present invention, there is provided a functional food composition comprising the above-described ginseng hydrolyzed concentrate or ginseng hydrolyzed powder as an active ingredient, wherein the ginseng hydrolyzed concentrate or hydrolyzed ginseng powder is characterized in that the content of IH-901 exceeds 36 mg / g Can provide a functional food composition containing high concentrations of IH-901 and simultaneously containing Rh2, F1 and F2 not found in ginseng. In the present invention, the content of IH-901 in the ginseng hydrolyzed concentrate or the ginseng hydrolyzed powder is preferably 40 to 50 mg / g, more preferably 45 to 50 mg / g. In addition to IH-901, it also contains ginsenosides Rh2, F1 and F2 as metabolites, which are effective in preventing immune enhancement, antidiabetic and anti-cancer effects, as well as inhibiting senescence (recovery of testicular sperm count, motility etc.) A functional food composition having an improving effect can be provided.

The functional food composition according to the present invention can be prepared by directly adding the ginseng hydrolyzed concentrate or ginseng hydrolyzed powder or a mixture thereof, or by processing according to a method known to a person skilled in the art, and further adding other food ingredients. The added amount of the ginseng hydrolyzed concentrate or the ginseng hydrolyzed powder can be suitably determined by a person skilled in the art according to the intended use of the functional food composition. In general, the ginseng hydrolyzed concentrate or the hydrolyzed ginseng powder of the present invention may be contained in an amount of 0.1 to 100% by weight based on the total weight of the functional food composition. However, in the case of long-term intake for the purpose of controlling health, the content may be less than the above range, and since there is no problem in terms of safety, the active ingredient may be used in an amount exceeding the above range.

The food ingredient that can be added to the functional food composition according to the present invention may be varied depending on the kind of the functional food. If the ingredient does not cause side effects when mixed with the ginseng hydrolyzate or the ginseng hydrolyzate according to the present invention, It does not.

In addition, the functional food composition according to the present invention can be used in the form of soft or hard capsules, tablets, granules, droppings and teabags filled with the composition in the form of dry powders according to a conventional method, and can be used for immunization, antidiabetic, , Antioxidants, and skin wrinkles. Examples of the food to which the composition can be added include, but are not limited to, various foods, beverages, gums, candies, confectioneries, tea, vitamin complexes, and health functional foods.

Hereinafter, the present invention will be described in more detail by way of examples.

Example  One

Five grams of each of ginseng and white ginseng was extracted 4 times at 80 ° C for 3 hours with a mixture of water and ethanol (1: 1 weight ratio, 5 times the weight of the raw material), rapidly cooled to 4 ° C at a rate of 5 ° C / min, , And 0.3 g and 0.5 g of pectinase and cellulase were added to 7 g of the concentrated ginseng concentrate and hydrolyzed at 50 ° C and pH 4 to 5 (adjusted with citric acid) for 24 hours. The mixture was concentrated under reduced pressure at 80 ° C to obtain ginseng 8.5 g of a hydrolyzed concentrate was prepared.

Example  2

7 g of a ginseng hydrolyzed concentrate was prepared in the same manner as in Example 1, except that 8.1 g of the extract obtained by extracting 10 g of ginseng in a solvent in Example 1 was used.

Example  3

1 kg of white ginseng was immersed, hydrolyzed and concentrated in the same manner as in Example 1 using 5 L of ethanol to prepare 7.5 g of a ginseng hydrolyzed concentrate.

Example  4

6 g of fresh ginseng was finely pulverized and sterilized, suspended in water, adjusted to pH 3 to 4.5, 0.3 g and 0.5 g of pectinase and cellulase were added, respectively, and the reaction time was 72 hours. After completion of the reaction, the reaction solution was taken and immersed for 12 hours. In addition, 30 ml of water was added to the hydrolyzed ginseng in which the supernatant liquid was separated, and extracted four times at 80 ° C. and immersed for 12 hours. Immersing the reaction solution and hydrolyzing The supernatant liquid was immersed in distilled water at 80 ° C. to obtain 3.8 g of a ginseng hydrolyzed concentrate Could.

Example  5

7 g of the ginseng powder was suspended in water to adjust the pH to 3 to 4.5, and then immersed, hydrolyzed and concentrated in the same manner as in Example 4 to prepare 3.9 g of a ginseng hydrolyzed concentrate.

Example  6

The ginseng hydrolyzed concentrate prepared in Example 1 was dried at 80 ° C under reduced pressure to prepare 4.5 g of ginseng hydrolyzed powder.

Example  7

The ginseng hydrolyzed concentrate prepared in Example 5 was dried at 80 ° C under reduced pressure to obtain 2.8 g of ginseng hydrolyzed powder.

Comparative Example  One

5.7 g of a ginseng hydrolyzed concentrate was prepared in the same manner as in Example 1, except that 1.3 g of the enzyme ropptinase alone was used in Example 1.

Comparative Example  2

5.6 g of a ginseng hydrolyzed concentrate was prepared in the same manner as in Example 1, except that 1.3 g of the cellulase alone was used as the enzyme in Example 1.

Comparative Example  3

5.9 g of a ginseng hydrolyzed concentrate was prepared in the same manner as in Example 1, except that 0.3 g of enzyme ropptinase and 1 g of beta-galactosidase were used in Example 1.

Comparative Example  4

6 g of a ginseng hydrolyzed concentrate was prepared in the same manner as in Example 1, except that the ginseng extract in Example 1 was concentrated without rapid cooling and immersion.

Experimental Example

In order to confirm the contents of IH-901 and ginsenoside components in the ginseng hydrolyzed concentrate of the ginseng hydrolyzed concentrate and the ginseng hydrolyzed powder according to the present invention, the ginseng hydrolyzed concentrate prepared according to Example 1 and Comparative Examples 1 to 4 , 1 g of saturated butanol was extracted three times with 60 ml of saturated butanol, and 120 mg of the concentrate obtained by concentrating the butanol fraction was dissolved in methanol, and the content was analyzed by high pressure liquid chromatography. The results are shown in the following Table 1 (Unit: mg / g). For comparison, the results of the measurement and analysis of the ginseng extract before concentration in Example 1 are also shown.

As shown in Table 1, the ginseng hydrolyzed concentrate according to the present invention not only contains IH-901, which is not present in the ginseng extract at a much higher level than conventional levels (up to 36 mg / g level), but also ginsenoside Rh2 , F1 and F2 were further contained.

On the other hand, when pectinase or a cellulase is used alone (Comparative Examples 1 and 2), or when pectinase and beta-galactosidase are mixedly used as in the prior art (Comparative Example 3), the low temperature immersion step , There is no particular effect on the increase of the content of IH-901, and even when mixed with a pectinase and a cellulase, it is difficult to expect a significant improvement in the content level of the conventional IH-901 unless a low temperature immersion process is used.

The preferred embodiments of the present invention have been described in detail above. It will be understood by those of ordinary skill in the art that various changes in form and details may be made therein without departing from the spirit and scope of the invention as defined by the appended claims.

Accordingly, the scope of the present invention is defined by the appended claims rather than the foregoing detailed description, and all changes or modifications derived from the meaning, range, and equivalence of the claims are included in the scope of the present invention Should be interpreted.

Claims (11)

(a1) preparing ginseng extract by adding water or an organic solvent to ginseng;
(b1) rapidly cooling the ginseng extract prepared in the step (a1) at a temperature of 0 to 10 ° C at a rate of 1 to 10 ° C / min and then immersing the ginseng extract for 1 to 12 hours;
(c1) adding the pectinase and the cellulase to the supernatant of the ginseng extract immersed in the step (b1), and then hydrolyzing the mixture; And
(d1) concentrating the ginseng extract hydrolyzed in the step (c1);
≪ / RTI > (a2) adding ginseng to water or an organic solvent to prepare a ginseng solution;
(b2) adding the pectinase and the cellulase to the ginseng solution prepared in the step (a2), and then hydrolyzing the mixture;
(c2) separating the hydrolyzed ginseng solution and the hydrolyzed ginseng in the step (b2);
(d2) adding water or an organic solvent to the hydrolyzed ginseng in which the ginseng liquid has been separated in step (c2);
(e2) rapidly cooling the ginseng solution separated in the step (c2) and the extract solution extracted in the step (d2) from 0 to 10 ° C at a rate of 1 to 10 ° C / min and then immersing for 1 to 12 hours; And
(f2) concentrating the supernatant of the extract solution immersed in step (e2);
≪ / RTI > 3. The method according to claim 1 or 2,
Wherein the ginseng in step (a1) or step (a2) is selected from the group consisting of ginseng, white ginseng, fresh ginseng, dried ginseng, red ginseng, taekguk ginseng, extracts thereof, powder thereof, and combinations thereof. Way. 3. The method according to claim 1 or 2,
In the step (c1) or (b2), the content of the pectinase and the amount of the cellulase is 0.1 to 100 parts by weight of the cellulase relative to 1 part of the pectinase, Wherein the mixture of the pectinase and the cellulase is added in an amount of 0.1 to 50 parts by weight based on 100 parts by weight of the ginseng extract or ginseng solution. 3. The method according to claim 1 or 2,
Wherein the hydrolysis of step (c1) or step (b2) is performed at a pH of 3 to 8 and at a temperature of 10 to 70 ° C for 1 to 72 hours. delete A process for producing a ginseng hydrolyzed powder according to claim 1 or 2 by vacuum drying or lyophilization. 3. The method according to claim 1 or 2,
Wherein the ginseng hydrolyzed concentrate has an IH-901 content of more than 36 mg / g. 8. The method of claim 7,
Wherein the ginseng hydrolyzed powder has an IH-901 content of more than 36 mg / g. 9. The method of claim 8,
Wherein the ginseng hydrolyzed concentrate contains ginsenosides Rh2, F1 and F2. 10. The method of claim 9,
Wherein the ginseng hydrolyzed powder contains ginsenosides Rh2, F1 and F2.