CN106726889A - The extracting method of Camellia nitidissima active ingredient and prepare the purposes of health products - Google Patents
The extracting method of Camellia nitidissima active ingredient and prepare the purposes of health products Download PDFInfo
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- CN106726889A CN106726889A CN201611093570.5A CN201611093570A CN106726889A CN 106726889 A CN106726889 A CN 106726889A CN 201611093570 A CN201611093570 A CN 201611093570A CN 106726889 A CN106726889 A CN 106726889A
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/02—Preparations for care of the skin for chemically bleaching or whitening the skin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/08—Anti-ageing preparations
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
- A61K2800/805—Corresponding aspects not provided for by any of codes A61K2800/81 - A61K2800/95
Abstract
The invention discloses a kind of method of the effective component extracting from rare species Camellia nitidissima dry leaves or dried flower, be efficiently extract with small-micelle water, golden flower camellias and leaf extract prepared by the modern biotechnology such as film engineered molecule retention, rich in the antioxidant contents such as polyphenol, flavones and anti-inflammation composition, extract and separate yield high, can be used to prepare medicine, food and beverage.
Description
Technical field
The present invention relates to a kind of method of the effective component extracting from plant, particularly from rare species Camellia nitidissima dry leaves
Or the method for effective component extracting and prepare the purposes of health products in dried flower.
Background technology
Camellia nitidissima, Classification system（Camellia nitidissima C. W. Chi）；Camellia nitidissima belongs to Theaceae, camellia
Category, nineteen sixty finds first in China；Camellia nitidissima is a kind of ancient plant, extremely rare, is distributed extremely narrow, the whole world
90% wild Camellia nitidissima is only distributed in the blue band of mountain offshoot one of Zhuang National Minority of China autonomous region Fangchenggang City Shiwan Dashan, growth
Below 700 meters of height above sea level, Nanning in Guangxi, Qinzhou City and Vietnam also find on a small quantity, but quantity is few；It is gold that Camellia nitidissima blooms
Yellow, it is dazzling brilliant, coating of wax is seemingly applied, it is sparkling and crystal-clear and glossy, seemingly there is translucent sense, it is precious plant rare in the world
Thing, foreign countries are referred to as magical " Dongfang " magic tea, are described as " plant kingdom giant panda ", " tea race queen ".
Through inspection show, the nontoxic level of golden flower Camellias, containing more than 400 kinds of nutriments, have no toxic side effect.Particularly Camellia nitidissima
Rich in tea polysaccharide, Tea Polyphenols, Total saponin, general flavone, Tea Pigment, caffeine, protein, vitamin B1, B2, vitamin C, dimension life
Various natural nutrition compositions such as plain E, folic acid, aliphatic acid, B- carrotene；Also contain tens kinds of amino such as theanine, threonine
Acid, and rich in there is various natural organic germaniums to human body with important health-care effect（Ge）, selenium（Se）, molybdenum（M）, zinc（Zn）、
Vanadium（V）Deng trace element, and potassium（K）, calcium（Ca）, magnesium（Mg）Deng macroelement.Among the people is " refreshing tea " also known as Camellia nitidissima；Camellia nitidissima
With obvious hypoglycemic and glucose in urine effect, can effectively improve diabetes " three high " symptom.Camellia nitidissima effectively reduce blood sugar,
While blood pressure, can effective reduction blood fat, improvement cause by hypertension it is various be not suitable with symptom, reduce cholesterol in serum
With B- lipoprotein, promote insulin secretion, strengthen immunity, regulating blood flow amount, atherosclerosis are anti-inflammation, clear
Thermal detoxification, defaecation diuresis are dried, and are promoted liver metabolism, anti-cancer and are suppressed tumour growth etc..Additionally, our research indicate that, golden flower
Tea has obvious action for improving internal acetaldehyde dehydrogenase, therefore Camellia nitidissima is had into raising alcohol dehydrogenase with other
The material matching of activity, can play quickening alcohol and decompose, and alleviate the effect of poisoning symptom after drinking.
Camellia nitidissima while blood sugar, blood pressure is effectively reduced, can effective reduction blood fat, improve cause by hypertension each
Plant and be not suitable with symptom, reduce cholesterol in serum and B- lipoprotein, promote insulin secretion, strengthen immunity, regulating blood flow amount,
Atherosclerosis, anti-inflammation, clearing heat and detoxicating, defaecation diuresis are dried, and are promoted liver metabolism, anti-cancer and are suppressed tumour growth
Deng.General flavone content 4400mg/kg in Camellia nitidissima, flavone compound have lowering blood pressure and blood fat, prevent thrombosis,
The effects such as preventing and treating cardiovascular and cerebrovascular disease, immune enhancing, reduction fragility of blood vessels, improvement cardiovascular and cerebrovascular blood circulation, can be with antibacterial
And antiviral, anti-oxidant, anti-aging, radioresistance, cough-relieving, eliminating the phlegm, and anti-toxin for liver.Selenium is human erythrocyte GSH-PX activity
The composition of enzyme, he has anti-oxidant, protects cell membrane, protects cardiovascular effect.Chinese Scientists also found that selenium is treatment Keshan
The active drug of disease and Kaschin-Beck disease, additionally, selenium also has function of detoxification, reduces the toxicity of yellow song B1, to some chemical carcinogens
Matter has attenuation, moreover it is possible to mitigate infringement of the toxin to liver cell.Germanium can reduce the biotic potential of cancer cell, change its physiology shape
State, suppresses the increment growth of cancer cell, can also induce the interferon of cancer-resisting substance in human body, can induce macrophage and be changed into anti-
Cancer cell, therefore someone uses germanium treating cancer, germanium is to vascular sclerosis in addition, cerebral thrombus, cerebral hemorrhage, kidney hepatopathy, stomach trouble, diabetes,
Arthritis, chronic neuralgia also has obvious curative effect.The effect of golden flower camellias, can astringing to arrest bleeding, master have blood in stool；Menorrhalgia,
It is especially advantageous to women.
It is as follows on the document from Camellia nitidissima effective component extracting：
1st, Chinese patent, the method that polyphenols is extracted from golden camellia tea, application number：CN201510573856.2 applies
Day：2015.09.10 publication number： CN105168593A ；Applicant：Medical Colleges Of Guilin makes a summary：The invention provides from
The method that polyphenols is extracted in golden camellia tea, including：1) golden camellia tea ethanol is extracted, filtering takes filtrate standby；2) will
Filtrate crosses the hollow-fibre membrane that molecular cut off is 5000, obtains first time concentrate and first time permeate；Will be dense for the first time
Contracting liquid crosses the hollow-fibre membrane that molecular cut off is 20,000, obtains second permeate and second concentrate, and will be dense for the second time
It is 100,000 that contracting liquid crosses molecular cut off, obtains third time permeate；3) by low pole macroporous absorbent resin in first time permeate
Post, first washes post to colourless, then with ethanol elution, collects ethanol eluate；4) by nonpolar macroporous suction in third time permeate
Attached resin column, first washes post to colourless, then ethanol elution, collects ethanol eluate；5) by step 3) and step 4) eluent
Mixing, concentration is dried, and obtains final product polyphenols.The polyphenol substance that the present invention is extracted has extremely strong anti-liver cancer efficacy.
2nd, Chinese patent, a kind of golden flower tea extracts, application number：The CN03140885.0 applyings date：2003.06.05
Publication number：CN1476767A applicant：Guangxi Fuxin Science ＆ Technology Co., Ltd. makes a summary：Disclosed by the invention is a kind of
Golden flower tea extracts.Contain many compositions beneficial to human body in Camellia nitidissima, but be not fully used for a long time, it is existing
Extraction golden flower tea components method be high temperature extract and concentrate, so can partly destroy its active ingredient, the present invention uses alcohol
Carry and being combined with water extraction, and use ultrasonic oscillation, to improve extraction efficiency, overall process employs the low temperature bar less than 60 DEG C
Part, effectively protects the aromatic substance in Camellia nitidissima and the various compositions with healthcare function, extraction rate reached to more than 90%,
And extraction time can be shortened, reduce cost.
3rd, Chinese patent, the method that camellia chrysantha polysaccharide is extracted from golden camellia tea, application number： CN201510707853.3
The applying date：2015.10.27 publication number：CN105175569A, applicant：Medical Colleges Of Guilin makes a summary：The present invention is provided
The method that camellia chrysantha polysaccharide is extracted from golden camellia tea, comprises the following steps：1) by golden camellia tea extracting in water, water extraction is obtained
Liquid；2) after Aqueous extracts are concentrated, ethanol precipitation is added, taking precipitate is standby；3) by step 2) sediment use anhydrous second successively
Alcohol, acetone washing, then add distillation water dissolves, add protease hydrolyzed, obtain enzymolysis liquid；4) enzymolysis liquid is placed in into frequency is
10~60s is processed under 1000~5000kHz ultrasonic waves, is then dialysed through the bag filter that molecular cut off is 10000, gone bail for and stay liquid
Dialysed through the bag filter that molecular cut off is 500000, take permeate concentration, add ethanol precipitation, taking precipitate is standby；5)
By step 4) described in sediment washed with absolute ethyl alcohol, acetone successively, then plus distillation water dissolves, activated carbon decolorizing, filtering,
Dry, as camellia chrysantha polysaccharide.The camellia chrysantha polysaccharide that the present invention is obtained has preferable immunocompetence.
4th, Chinese patent, a kind of camellia nitidissima concentrate, application number：CN03149225.8, the applying date：2003.06.18, it is public
The number of opening：CN1460438A, applicant：Guangxi Fuxin Science ＆ Technology Co., Ltd., summary：The invention discloses a kind of golden flower
Tea concentrate.Concentration to golden flower tea extracts at present employs conventional high temperature extraction method for concentration, at high operating temperatures,
Easily make the aromatic substance in Camellia nitidissima volatilize to scatter and disappear, or even active component is subject to compared with havoc.The present invention is used
Nanometer UF membrane concentration technique can be concentrated under the conditions of 25-40 DEG C to golden flower tea extracts, it is to avoid heating is concentrated
Destruction of the journey to active ingredient, and effective ingredient loss.The present invention is to carry out the basis of Camellia nitidissima series product development, can be with
Further develop Camellia nitidissima food, beverage, health products, commodity and medicine.
5th, Chinese patent, a kind of Camellia nitidissima flavonoid glycoside and its production and use, application number：
The CN201210045032.4 applyings date：2012.02.24 publication number：CN102617672A, applicant：University Of Dalian
Summary：The present invention relates to anticancer compound Camellia nitidissima flavone glycosides A of separation and preparation method thereof in a kind of camellia from golden flower and use
On the way.The present invention monomeric compound and cut isolated from the acetic acid ethyl ester extract of the ethanol extract of golden flower camellias 95%
Show predominantly flavones ingredient through qualitative, quantitative determination, and the chemistry of the compound is determined through the analysis of modern spectral technique
Structure, chemistry is entitled：Quercetin -7-O- (6 "-O-E- coffee acyls)-β-D-Glucose glycosides.Show through anticancer experiment in vitro,
The compound can substantially suppress tumor cell proliferation, and the activity with inducing apoptosis of tumour cell.The invention product possesses anti-
The potentiality to be exploited of tumour medicine.
6th, a kind of Chinese patent, CO2 supercritical methanol technologies extract the process application number of Camellia nitidissima leaf flavonoids：
The CN201410000082.X applyings date：2014.01.02 publication number：CN103705647A, applicant：Qingdao agricultural is big
Learn summary：The invention discloses the process that a kind of CO2 supercritical methanol technologies extract Camellia nitidissima leaf flavonoids, it includes following step
Suddenly：1) Camellia nitidissima is crushed to 20~100 mesh, is placed in extractor；2) it is with 55~85% ethanol as agent, supercritical CO 2 is carried
Solvent；3）35~55 DEG C of extractor temperature, 10~20L/h of pressure 25~35MPa, CO2 flow；25 DEG C of one-level knockout drum temperature,
6~8MPa of pressure；25 DEG C of the second-order separation tank temperature, pressure 6MPa；2~4h of extraction time；The second of golden camellia tea general flavone can be obtained
Alcoholic solution；4) ethanol is reclaimed in vacuum distillation, and freeze-drying obtains final product auburn golden camellia tea total flavonoids extract.Carried using the method
The general flavone for taking, organic solvent-free residual is simple and easy to do, and high income, and yield can be widely applied to food, medicine up to 3.81%
The fields such as product, can be obviously improved the economic benefit and social benefit of Camellia nitidissima.
7th, Chinese patent, golden flower Flos Camelliae Japonicae extracts and its preparation method and application, application number： CN201510267354.7
The applying date：2015.05.22 publication number：CN104958440A, 2015.10.07 applicant：Dalian University of Technology plucks
Will：The invention discloses golden flower Flos Camelliae Japonicae extracts, preparation method is to mix golden flower camellias with oyster shell whiting, and micro- shear-auxiliary agent is mutual
Make technology mechanical crushing, extracted using water or ethanol, the Extracting temperature is 80~100 DEG C；Golden flower Flos Camelliae Japonicae extracts contain 20-
The total saposins of the general flavone of 40wt%, the carbohydrate of 20-40wt%, the Tea Polyphenols of 1-10wt% and 1-10wt%；The present invention is also carried
For application of the golden flower Flos Camelliae Japonicae extracts in treatment or prevention NASH medicine is prepared.Golden flower Flos Camelliae Japonicae extracts of the present invention
The fatty liver level of NASH can be mitigated, TG, the TC in blood and liver are decreased, and toxic and side effect
It is small；There is preferable preventive and therapeutic effect to alcoholic fatty liver.
8th, Chinese patent, a kind of Camellia nitidissima flavonoid glycoside and its production and use application number：
The CN201410206858.3 applyings date：2014.05.15 publication number：CN103951723A, applicant：University Of Dalian
Summary：The present invention relates to a kind of antianaphylaxis asthma compound Camellia nitidissima flavonoid glycoside for from golden camellia tea separate and its preparation side
Method and purposes.The water extract of golden camellia tea is passed sequentially through silica gel column chromatography and Sephadex LH-20 column chromatographys point by the present invention
From monomeric compound is obtained, the chemical constitution of the compound is determined through the analysis of modern spectral technique, such as formula I, its chemistry is entitled：
[α-L- rhamanopyranosyls (1 → 6)]-(2-O-E- is to coumaric acyl-β-D-Glucose for Kaempferol 3-O- β-D-Glucose base (1 → 3)
Glycosides).Show through the experiment of external antiallergy, the compound can substantially suppress lipoxygenase activity and suppress leukotriene generation.The present invention
The compound of formula I possesses the potentiality to be exploited of antianaphylaxis asthmatic medicament.
9th, Chinese patent, golden camellia tea active site, Its Preparation Method And Use application number：
The CN201310070480.4 applyings date：2013.03.06 publication number：CN104027477A, applicant：Guangxi medical courses in general are big
Learn summary：Disclose golden camellia tea active site, Its Preparation Method And Use.Preparation method includes：A. infiltrate：Take golden flower
Coarse tea leaf powder, with 5 times of alcoholic solution soaked overnights of (V/W) 30% or more concentration；B. extract：With with same concentrations in step A
Alcoholic solution to the golden camellia tea meal heating extraction 2 times or more than 2 times in step A, alcoholic solution consumption is golden camellia tea meal
10~40 times (V/W), each extraction time be 1~4 hour, filtering, merge extract solution, be concentrated under reduced pressure, recycling design is obtained slightly
Extract, filtering, takes filtrate, and it is 1.05~1.25 to obtain liquid proportion；C. macroporous absorbent resin treatment：The filtrate in step B is taken, is passed through
Macroporous resin column chromatography is separated, and successively with 10 times of the 10% of column volume, 30%, 80%, 95% ethanol water elutions, is collected respectively each
Elution fraction, by 80% ethanol water elution fraction vacuum distillation；The macroreticular resin be selected from AB-8, D101, D201, D140,
D141A, D301, D401, HP20 and HPD-100；D. dry：The 80% ethanolic moiety freeze-drying that step C is obtained, obtains final product.
10th, Chinese patent, Camellia nitidissima leaf extract and application thereof, application number：CN201410604971.7, publication number：
CN104274611A applicant：Fangchenggang Wanjing Forestry Co., Ltd. makes a summary：A kind of Camellia nitidissima leaf extract and its
Purposes.The extract from Camellia nitidissima leaves of plants, the mass percent of its significant component Tea Polyphenols for 3.5%~
20%, its preparation method is, will be filtered after the leaves of plants extracting in water of Camellia nitidissima, the filter residue also mistake after the extracting in water of 1~5 time
Filter, merges gained filtrate, and drying under reduced pressure obtains product；The process of leaves of plants and filter residue extracting in water is：Add 5~30 times of weights
The water of amount, ceases fire after being heated to boiling, and 10~180min of hot dipping ceases fire after being again heated to boiling, 1~180min of hot dipping.Should
Extract is mainly the Polyphenols extract of golden camellia tea, and preparation method is simple and easy to apply, suitable industrialized production；And the Camellia nitidissima
The hypoglycemic effect of leaf Polyphenols extract significantly, can be used to prepare hypoglycemic medicine, food, health products, or prepare treatment
IGT, insulin resistance, metabolic syndrome, the medicine of relevant disease caused by essential hyperglycaemia, it is applied widely.
11st, Chinese patent, a kind of preparation method of golden camellia tea pigment, application number：CN201410541226.2, application
Day：2014.10.14 publication number：CN104306559A, applicant：Wang Huiying makes a summary：The present invention discloses a kind of golden flower
The preparation method of tealeaves pigment, step is as follows：1) dried leaf of Camellia nitidissima is crushed to 40~120 mesh, it is small to soak 1~5 with hydro-thermal
Shi Hou, after common filter-cloth filtering, filtrate pore size filter is 0.1~0.5 μm of micro-filtration membrane microfiltration, and gained filtrate is coarse extraction
Liquid；2) crude extract is crossed into macroporous absorbent resin chromatographic column, with 50%~80% alcoholic solution elution chromatography post, collects wash-out
Liquid, eluent is concentrated under reduced pressure into the 30%~40% of original volume, and concentrate is placed in reactor, the oxidation of reactor blowing air,
The converted starch of its weight 0.5%~1.5% is added in concentrate after oxidation, golden camellia tea pigment is obtained after spray drying.
The present invention can fully extract Tea Pigment and catechin is converted into Tea Pigment, so that dark brown cellulose content extract high is obtained, should
Method is simple and easy to control, is easy to promote, and can be that the exploitation of golden camellia tea goes out new way.
12nd, Chinese patent, the extracting method of camellia chrysantha polysaccharide, application number：The CN201510705138.6 applyings date：
2015.10.27 publication number：CN105153324A, applicant：Medical Colleges Of Guilin, summary：The invention provides a kind of gold
The extracting method of jasmine tea polysaccharide, comprises the following steps：1) by golden camellia tea extracting in water, Aqueous extracts are obtained；2) it is Aqueous extracts are dense
After contracting, ethanol precipitation is added, taking precipitate is standby；3) by step 2) sediment washed with absolute ethyl alcohol, acetone successively, then
Plus distillation water dissolves, protease hydrolyzed is added, obtain enzymolysis liquid；4) enzymolysis liquid is placed under frequency ultrasonic wave and is processed, then
Dialysed through the bag filter that molecular cut off is 18000~28000, it is 400000~500000 through molecular cut off to go bail for and stay liquid
Bag filter is dialysed, and takes permeate concentration, adds ethanol precipitation, and taking precipitate is standby；5) by step 4) described in sediment according to
It is secondary to be washed with absolute ethyl alcohol, acetone, then add distillation water dissolves, activated carbon decolorizing, filtering is dried, as camellia chrysantha polysaccharide.This
The camellia chrysantha polysaccharide that invention is obtained has preferable immunocompetence.
13rd, Chinese patent, the highly effective extraction method of active ingredient and its answering in cigarette preparation in a kind of golden camellia tea
Use application number：CN200910040927.7 publication numbers：CN101607003A, applicant：Cigarette industry Limited Liability in Guangdong
Company makes a summary：The invention discloses a kind of highly effective extraction method of active ingredient in golden camellia tea and its in cigarette preparation
Using.The extracting method is comprised the following steps：Crush；Enzymolysis；Alcohol extracting；Separate；Concentration；The enzymolysis be with cellulase and
Protease is digested.Application of the extracting method in cigarette preparation is that the active ingredient of the golden camellia tea that will be extracted is dense
Contracting liquid is added in cigarette leaf group according to 0.1 ‰~10 ‰ mass ratio and prepares cigarette.The method that the present invention is provided is efficient, low
Cost is extracted active ingredient in golden camellia tea.The golden camellia tea active ingredient concentrate of extraction is applied to be made in cigarette preparation
Flue gas becomes softer continuous, fine and smooth, clear, and excitant has declined, and residual has been reduced in oral cavity, and returning sweet sense is remarkably reinforced,
There is the effect being obviously improved to cigarette quality.
14th, Chinese patent, golden camellia tea active site, Its Preparation Method And Use, application number：
The CN201310070499.9 applyings date：2013.03.06 publication number：CN104027478A, applicant：Guangxi medical courses in general are big
Learn summary：Disclose golden camellia tea active site, Its Preparation Method And Use.Preparation method includes：A. infiltrate：Take golden flower
Coarse tea leaf powder, with 5 times of alcoholic solution soaked overnights of (V/W) 30% or more concentration；B. extract：With with same concentrations in step A
Alcoholic solution to the golden camellia tea meal heating extraction 2 times or more than 2 times in step A, alcoholic solution consumption is golden camellia tea meal
10~40 times (V/W), each extraction time is 1~4 hour, and filtering merges extract solution, and recycling design obtains condensed cream；C. extract
Take：The condensed cream is suspended with water, successively with petroleum ether, ethyl acetate, extracting n-butyl alcohol, the extraction after ethyl acetate extraction is taken
Take liquid, recycling design；D. dry：The acetic acid ethyl ester extract freeze-drying that step C is obtained, obtains final product.
15th, Chinese patent, the method that polyphenol is extracted from golden camellia tea, application number：CN201510573558.3 Shens
Please day：2015.09.10 publication number：CN105055669A, applicant：Medical Colleges Of Guilin makes a summary：The invention provides
The method that polyphenol is extracted from golden camellia tea, including：1) golden camellia tea ethanol is extracted, filtering takes filtrate standby；2) by filtrate
The hollow-fibre membrane that molecular cut off is 3000 is crossed, first time concentrate and first time permeate is obtained；By first time concentrate
The hollow-fibre membrane that molecular cut off is 10,000 is crossed, second permeate and second concentrate is obtained, and by second concentrate
It is 80,000 to cross molecular cut off, obtains third time permeate；3) by low pole large pore resin absorption column in first time permeate, first
Washing post collects ethanol eluate to colourless, then with ethanol elution；4) by nonpolar macroporous adsorption resin in third time permeate
Post, first washes post to colourless, then ethanol elution, collects ethanol eluate；5) by step 3) and step 4) eluent mixing, it is dense
Contracting, dries, and obtains final product polyphenols.The polyphenol that the present invention is extracted has extremely strong anti-liver cancer efficacy.
16th, Chinese patent, Chinese medicine composition and its application, application number：The CN201210271906.8 applyings date：
2012.08.02 publication number：CN102764218A, applicant：Liuzhou Radiz Zanthoxyli Co., Ltd.;Radix zanthoxyli（Yangzhou）Wine
Shop articles for use Co., Ltd;AnHui LiangMianZhen Fangcao Daily Chemical Co., Ltd makes a summary：The invention discloses a kind of Chinese medicine composition
And its application, Chinese medicine composition of the invention is by Camellia nitidissima subset natural mineral matter water extract, grape pip subset
Natural mineral matter water extract, Radix Glycyrrhizae subset natural mineral matter water extract, the natural mineral matter water extraction of Chinese-Angelica micromolecular group
Thing, bletilla striata subset natural mineral matter water extract and ganoderma lucidum subset natural mineral matter water extract is taken to be mixed to get,
Chinese medicine composition of the invention can be used for flesh and slip pale skin external preparation, can multi-direction synthetic effect skin, and contain day
Functional component is penetrated epidermis implantation skin by right subset implant, has obvious whitening, crease-resistant, moisturizing simultaneously to skin
Etc. multiple effect, the natural traditional Chinese medicine composition can reduce the excitant of external preparation, strengthen the protective effect to skin, also with making
With safe and reliable, simple for production advantage.
Document disclosed above from different perspectives to extract Camellia nitidissima active ingredient discussed, have water extraction, alcohol extracting method,
Ethyl acetate extraction, CO 2 supercritical extraction method, membrane separation concentration method, Middle hollow fiber membrane method and enzymatic isolation method etc.,
The Camellia nitidissima active ingredient of different purposes is obtained, but there is an extraction method to be only limited to obtain fractions, useful component is in operation
During be lost in or waste, valuable resource fails to make full use of.
The content of the invention
It is an object of the invention to provide a kind of side of the effective component extracting from rare species Camellia nitidissima dry leaves or dried flower
Method, be efficiently extract with small-micelle water, dry golden flower camellias prepared by the modern biotechnology such as film engineered molecule retention
With leaf extract, rich in the antioxidant contents such as polyphenol, flavones and anti-inflammation composition, extract and separation yield is high, can be used to make
Standby medicine, foods and cosmetics.
The technical proposal of the invention is realized in this way：
1st, a kind of method of the effective component extracting from plant Camellia nitidissima dry leaves or dried flower, it is characterised in that：Process is as follows：
1）Alkaline small group water is obtained first standby；Described alkaline small rolls into a ball pH 9-13；
2）Collection Camellia nitidissima leaf and flower, remove impurity removing, standby；
3）Dry Camellia nitidissima leaf and dried flower piece are smashed with pulverizer respectively；
4）It is put into respectively in the Camellia nitidissima leaf or flower smashed using the alkaline small group water of pH 9-13, per kilogram golden flower
Tealeaves or flower add 8-10 to rise alkaline small group water；Soaking temperature at 1-10 DEG C, time 3-5 hours, then in 10-20
DEG C temperature for the first time extract 3-5 hours, isolate extract solution and slag；The extract solution of Camellia nitidissima leaf or flower is individually deposited；
5）It is put into slag respectively using pH 9-13 alkaline smalls group water；Per kilogram Camellia nitidissima leaf slag or flower slag add 5-8
Rise alkaline small group water；In 10-20 DEG C of temperature control second extraction 2-3 hours, alkaline extract solution and slag are isolated；Camellia nitidissima
The extract solution of leaf or flower is individually deposited；
6）It is put into slag respectively using the alkaline small group water of pH 9-13；Per kilogram Camellia nitidissima leaf slag or flower slag are added
3-5 rises alkaline small group water；Extracted 1-2 hours in 10-20 DEG C of temperature third time, isolate alkaline extract solution and slag；Gold
The extract solution of jasmine tea leaf or flower is individually deposited；
7）By alkaline extract solution respectively cross milipore filter, ultrafiltration membrane aperture between 0.05um to 1nm, usual molecular cut off scope
1000~300000, then with the ethanol elution of volumetric concentration 70-90%；Obtain the eluent of Camellia nitidissima leaf or flower；By
Ethanol and concentration are reclaimed, Camellia nitidissima leaf or flower dry cream is obtained.
The preparation of the alkaline small group water of described pH 9-13 is that pure water is put into electrolytic cell, just extremely graphite, is born
Extremely platinum；
In electrolysis, the chemical equation of the two poles of the earth generation is：
On negative electrode：2H2O+2e=2OH-+H2
On anode：2H2O-4e=4H++O2。
Described pure water is, with natural mineral water or municipal tap water as water source, three roads to be carried out to running water with upper filter mistake
Filter carries out purification process and obtains the distilled water obtained after pure water, or distillation treatment.
Above-described electrolytic process operating temperature is 50-80 DEG C, and operating current is 0.6-2.0 A/cm2, after electrolysis so
Afterwards alkaline small group water is collected from one side of negative electrode.
Although in above-mentioned background technology, document 16, Chinese Patent Application No.：CN201210271906.8 reports Chinese medicine group
Compound wherein has Camellia nitidissima subset natural mineral matter water extract, but does not refer to Camellia nitidissima subset natural mineral matter
How water extract prepares, and does not also refer to source and the physical property of subset natural mineral matter water.Wen Zhongti
To grape pip subset natural mineral matter water extract, Radix Glycyrrhizae subset natural mineral matter water extract, Chinese-Angelica micromolecular
Roll into a ball natural mineral matter water extract, bletilla striata subset natural mineral matter water extract and ganoderma lucidum subset natural mineral matter water
Extract also has no its extracting method, and the public does not know how subset natural mineral matter water obtains yet.
The present invention using subset abundance, be not limited to natural mineral water, can with municipal tap water as water source,
After three road above filter element filterings, absorption etc. are carried out to running water carrying out purification process, or distillation treatment, then by electrolyzer electric
Solution is obtained.
During electrolytic tank electrolysis, due to the effect of hydrogen bond, running water is typically to be made up of 13-15 subset,
When water enters electrolytic cell, the hydrogen bond of hydrone is opened in the presence of electric field, generates by the 5-6 molecular small molecule of moisture
Group's water.Meanwhile, in the presence of electric field, the partial cation Ca in solution2+、Mg2+、K+、H+It is electric according to obtaining Deng to movable cathode
There is reduction reaction in the power of sub- ability, H ions, obtain electronics and become hydrogen, and due to the reduction of H ion concentrations, hydroxide ion is dense
Degree is then raised, and water body is in alkalescent, and pH value is more than 7, and the water of this pole generation is referred to as alkaline water.Simultaneously in anode, anionic part
Cl-、SO42-、NO3-、NO2-、OH-Moved Deng Ghandler motion on the sunny side, in numerous anion, the most volatile de-electromation of hydroxide ion then occurs
Oxidation reaction, loses electronics and produces oxygen and water.Because hydroxide ion concentration reduction, H ion concentrations are then raised, water body is in weak
Acidity, pH value is less than 7, and the water of this pole generation is referred to as acid subset ionized water, and in opposite side, water after electrolyzed water machine by obtaining
To the alkalescent small-micelle water drunk；
Subset be 5,6 hydrones together, activity is high, it is easy to enter cell.It is easy to Absorption And Metabolism, dissolving power, infiltration
Power is strong, it may be said that be life running water.General water is all macromolecular mass, and usual 15 or so molecules together, are difficult by hydrogen bond
Into cell.And subset is 5, together, activity is high, it is easy to enter cell for 6 hydrones.The World Health Organization is to health
Pointed out in the judgment criteria of water：The breadth of water clusters half is less than 100 hertz, as vigorous small-micelle water.After testing,
Ching degree water clusters half ranges are a width of 74.07-79.48 hertz,
The present invention is also small molecule group water using the small-micelle water of alkalescence,（microcluster water, small
cluster water, micro clustered water）, effective component extracting in plant Camellia nitidissima dry leaves or dried flower,
It is a kind of extraordinary raw material, makes various health foods, its meaning is：People is aging year by year until the sick process died, is to thin
Born of the same parents' insufficient water, gradually acidification, cell lack nutrition to cause cell liquid（It is not that human body lacks nutrition）, and the mistake died young that becomes feeble and die
Journey.It is not sick that the mankind want good health and a long life, dies without known cause, as long as trying to allow the alkalescent water of more than pH7.35 of volume freely to go out
Enter cell, it is possible to accomplish, problem is just so simple.
By research, macromolecular mass water is not easily accessed cell, makes intracellular water fewer and feweri, increasingly acid.Small point
The hydrophilic channel that son group water can easily pass through 2 nanometers enters cell, brings nutrition into, takes sour poison out of, to taking good care of one's health, delays to decline
Always, the effect prevented and cured diseases is self-evident.
The water of subset has any benefit；Because the micel of water is smaller, the energy of water is bigger, the infiltration of water
Power, dissolving power, metabolism power are stronger.Because its micel is small, it is easier to pass in and out cell membrane, nutriment is transported, rubbish is taken out of, as most
Good transport agent, therefore say that the water of subset is the water for being best suitable for human body cell.
The expert of the World Health Organization found by many investigations, finally Changshou village residents ' health it is long-lived it is secret with
Local water is relevant.The several conditions for being best suitable for human body drinking water have been made according to the characteristics of the water quality of Changshou village.That is weak base, small
Micel, hexangle type, negative potential, oxygen carrying be strong, rich in the big physiologically active of trace element etc. six.
Acid small-micelle water and alkaline water clusters water are all beneficial to, and acid subset water process Camellia nitidissima flower is extracted with leaf
Thing, obtains rich in antioxidant contents such as polyphenol, flavones, with obvious hypoglycemic and glucose in urine effect, can effectively improve glycosuria
Sick " three high " symptom.
Alkalescent small-micelle water, can neutralize internal noxious acidic metabolite, drain harmful substance, be in body fluid
Alkalescent, reaches prevention and health-care effect.Micromolecular water has the negative potential higher than solid carbon dioxide fruit vegetable（-86~105mV）, therefore
Eubolism to body is highly beneficial, can remove free radical excessive in vivo, improves body immunity.
Substantive distinguishing features of the invention and significant progress are：
1st, the present invention rolls into a ball water from Camellia nitidissima leaf or the method for spending middle effective component extracting using the alkaline small of pH 9-13
It is put into respectively in the Camellia nitidissima leaf or flower smashed；Soaking temperature at 1-10 DEG C, then 10-20 DEG C three times extraction, by alkali
Property extract solution cross milipore filter respectively, ultrafiltration membrane aperture between 0.05um to 1nm, usual molecular cut off scope 1000~
300000, then with the ethanol elution of volumetric concentration 70-90%；The alkaline small group extract solution for being purified, as preparing facial mask
With the purposes in terms of skin care item.
Due to hydrone H2The effect of hydrogen bond between O, the light water in daily life is by 13-15 hydrone H2O composition compared with
Macromolecular mass water, due to this 13-15 hydrone H2The special construction of O, hydrone H2The zwitterion quilt that the faint ionization of O is produced
Cover mutually, water is in neutrality（PH7.0 or so）.When water enters electrolytic cell, in the presence of electric field, the hydrogen bond of hydrone is beaten
Open, the cation Ca in solution2+、Mg2+、K+、H+Deng to movable cathode, according to the power for obtaining electronic capability, H ions are reduced
Reaction, obtains electronics and becomes hydrogen, and due to the reduction of H ion concentrations, hydroxide ion concentration is then raised, and water is gradually partially in alkalescence, PH
Value is more than 7, and the generation of this pole is referred to as alkaline small group water by the 5-6 molecular water of moisture；Simultaneously in anode, anionic part
Cl-、SO42-、NO3-、NO2-、OH-Moved Deng Ghandler motion on the sunny side, in numerous anion, the most volatile de-electromation of hydroxide ion then occurs
Oxidation reaction, loses electronics and produces oxygen and water, and due to hydroxide ion concentration reduction, H ion concentrations are then raised, and water is gradually inclined
In acidity, pH value is less than 7, and the generation of this pole is referred to as acid small-micelle water by the 5-6 molecular water of moisture.
2nd, our in past when golden flower camellias and leaf natural product extraction is carried out, in order to obtain the function knot required for us
Structure component, generally requires to destroy the original institutional framework of animals and plants, and hydrolysis is exactly the extracting method that we generally use.Initially
People improve the hydrolysis efficiency of natural products by the means for adding strong acid, highly basic to improve extract polarity, thus obtain compared with
Extraction yield high, but the addition of strong acid and strong base makes troubles and endangers to production technology, also easily introduces potential to product
Hazard factor.Therefore, essence belongs to pure water, is just extracted as preferable natural products but also with highly polar small-micelle water
Liquid.So the present invention is compared with conventional soda acid extracting method, with extraction process it is gentle, it is safe and healthy the characteristics of.
3rd, the alkaline golden flower camellias and leaf extract for obtaining, rich in antioxidant contents such as polyphenol, flavones, with obvious drop
Blood sugar and glucose in urine are acted on, and can effectively improve diabetes " three high " symptom.Camellia nitidissima effectively reduce blood sugar, blood pressure while,
Can effective reduction blood fat, improvement cause by hypertension it is various be not suitable with symptom, reduce cholesterol in serum and B- lipoprotein,
Promote insulin secretion, strengthen immunity, regulating blood flow amount, atherosclerosis, anti-inflammation, clearing heat and detoxicating, defaecation
Diuresis is dried, and is promoted liver metabolism, anti-cancer and is suppressed tumour growth etc..Additionally, our research indicate that, Camellia nitidissima is for improving body
Interior acetaldehyde dehydrogenase has obvious action, therefore Camellia nitidissima and other raw materials with raising alcohol dehydrogenase activity are taken
Match somebody with somebody, quickening alcohol can be played and decomposed, alleviate the effect of poisoning symptom after drinking.
4th, the present invention processes golden flower tea fresh leaves and fresh flower using small-micelle water as extract with low temperature process, extracts work
Skill and equipment are uncomplicated, make full use of the active ingredient of complete golden flower camellias and leaf.Obtain high extraction efficiency.Its medicinal extract
Rate can bring up to more than 22% from common acids alkali extracting method from less than 15%.
5th, the alkaline golden flower camellias and leaf extract for obtaining, rich in polyphenol and anti-inflammation composition, as facial mask or cosmetic
Product, can the free radical piled up except skin cell metabolism of high-efficient cleaning, improve human detoxification and defensive enginery, make senile cell
Vigor recovered, accelerate to remove dead Skin Cell, promote the new life of Skin Cell, delaying skin aging.So that
Because the age increases caused face macula lutea and shoaling and then disappearing, pore shrinks, diminishing even disappear, and facial thickness wrinkle changes
It is kind.Long-term use can make skin delicate, smooth, and elasticity is recovered, is rich in youth gloss.
Specific embodiment
Embodiment 1,
1st, a kind of method of the effective component extracting from plant Camellia nitidissima dry leaves or dried flower, process is as follows：
1）Alkaline small group water is obtained first standby；Described alkaline small rolls into a ball pH 9-10；
2）Collection Camellia nitidissima leaf and flower, remove impurity removing, standby；
3）Dry Camellia nitidissima leaf and dried flower piece are smashed with pulverizer respectively；
4）It is put into respectively in the Camellia nitidissima leaf or flower smashed using the alkaline small group water of pH 9-10, per kilogram golden flower
Tealeaves or flower add 8-10 to rise alkaline small group water；Soaking temperature at 1-10 DEG C, time 3-5 hours, then in 10-20
DEG C temperature for the first time extract 3-5 hours, isolate extract solution and slag；The extract solution of Camellia nitidissima leaf or flower is individually deposited；
5）It is put into slag respectively using the alkaline small group water of pH 9-10；Per kilogram Camellia nitidissima leaf slag or flower slag are added
5-8 rises alkaline small group water；At 1-10 DEG C, alkaline extract solution and slag are isolated in second extraction 2-3 hours to soaking temperature；
The extract solution of Camellia nitidissima leaf or flower is individually deposited；
6）It is put into slag respectively using the alkaline small group water of pH9-10；Per kilogram Camellia nitidissima leaf slag or flower slag add 3-
5 liters of alkaline smalls roll into a ball water；Soaking temperature at 1-10 DEG C, extract 1-2 hours, isolates alkaline extract solution and slag by third time；Gold
The extract solution of jasmine tea leaf or flower is individually deposited；
7）By alkaline extract solution respectively cross milipore filter, ultrafiltration membrane aperture between 0.05um to 1nm, usual molecular cut off scope
1000~300000, then with the ethanol elution of volumetric concentration 70-90%；Obtain the eluent of Camellia nitidissima leaf or flower；By
Ethanol and concentration are reclaimed, Camellia nitidissima leaf or flower dry cream is obtained.
The preparation of the alkaline small group water of described pH 9-10 is that pure water is put into electrolytic cell, just extremely graphite, is born
Extremely platinum；
Described pure water is, with natural mineral water or municipal tap water as water source, three road above filter element filterings to be carried out to running water and is entered
Row purification process obtains the distilled water obtained after pure water, or distillation treatment.Described electrolytic process operating temperature is 50-80
DEG C, operating current is 0.6-2.0 A/cm2。
Embodiment 2,
A kind of method of the effective component extracting from plant Camellia nitidissima dry leaves or dried flower, process is as follows：
1）Alkaline small group water is obtained first standby；Described alkaline small rolls into a ball pH 10-12；
2）Collection Camellia nitidissima leaf and flower, remove impurity removing, standby；
3）Dry Camellia nitidissima leaf and dried flower piece are smashed with pulverizer respectively,
4）It is put into respectively in the Camellia nitidissima leaf or flower smashed using the alkaline small group water of pH 10-12, per kilogram golden flower
Tealeaves or flower add 8-10 to rise alkaline small group water；Soaking temperature at 1-10 DEG C, time 3-5 hours, then in 10-20
DEG C temperature for the first time extract 3-5 hours, isolate extract solution and slag；The extract solution of Camellia nitidissima leaf or flower is individually deposited；
5）It is put into slag respectively using the alkaline small group water of pH 10-12；Per kilogram Camellia nitidissima leaf slag or flower slag are added
5-8 rises alkaline small group water；In 10-20 DEG C of temperature control second extraction 2-3 hours, alkaline extract solution and slag are isolated；Gold
The extract solution of jasmine tea leaf or flower is individually deposited；
6）It is put into slag respectively using the alkaline small group water of pH 10-12；Per kilogram Camellia nitidissima leaf slag or flower slag are added
3-5 rises alkaline small group water；Extracted 1-2 hours in 10-20 DEG C of temperature third time, isolate alkaline extract solution and slag；Gold
The extract solution of jasmine tea leaf or flower is individually deposited；
7）By alkaline extract solution respectively cross milipore filter, ultrafiltration membrane aperture between 0.05um to 1nm, usual molecular cut off scope
1000~300000, then with the ethanol elution of volumetric concentration 70-90%；Obtain the eluent of Camellia nitidissima leaf or flower；By
Ethanol and concentration are reclaimed, Camellia nitidissima leaf or flower dry cream is obtained.
The preparation of the alkaline small group water of described pH10-12 is that pure water is put into electrolytic cell, just extremely graphite, is born
Extremely platinum；Described pure water is the distilled water of acquisition after distillation treatment.Above-described electrolytic process operating temperature is 50-
80 DEG C, operating current is 0.6-2.0 A/cm2。
Embodiment 3
A kind of method of the effective component extracting from plant Camellia nitidissima dry leaves or dried flower, process is as follows：
1）Alkaline small group water is obtained first standby；Described alkaline small rolls into a ball pH 11-13；
2）Collection Camellia nitidissima leaf and flower, remove impurity removing, standby；
3）Dry Camellia nitidissima leaf and dried flower piece are smashed with pulverizer respectively,
4）It is put into respectively in the Camellia nitidissima leaf or flower smashed using the alkaline small group water of pH 11-13, per kilogram golden flower
Tealeaves or flower add 8-10 to rise alkaline small group water；Soaking temperature at 1-10 DEG C, time 3-5 hours, then in 10-20
DEG C temperature for the first time extract 3-5 hours, isolate extract solution and slag；The extract solution of Camellia nitidissima leaf or flower is individually deposited；
5）It is put into slag respectively using the alkaline small group water of pH 11-13；Per kilogram Camellia nitidissima leaf slag or flower slag are added
5-8 rises alkaline small group water；In 10-20 DEG C of temperature control second extraction 2-3 hours, alkaline extract solution and slag are isolated；Gold
The extract solution of jasmine tea leaf or flower is individually deposited；
6）It is put into slag respectively using the alkaline small group water of pH 11-13；Per kilogram Camellia nitidissima leaf slag or flower slag are added
3-5 rises alkaline small group water；Extracted 1-2 hours in 10-20 DEG C of temperature third time, isolate alkaline extract solution and slag；Gold
The extract solution of jasmine tea leaf or flower is individually deposited；
7）By alkaline extract solution respectively cross milipore filter, ultrafiltration membrane aperture between 0.05um to 1nm, usual molecular cut off scope
1000~300000, then with the ethanol elution of volumetric concentration 70-90%；Obtain the eluent of Camellia nitidissima leaf or flower；By
Ethanol and concentration are reclaimed, Camellia nitidissima leaf or flower dry cream is obtained.
The preparation of the alkaline small group water of described pH 11-13 is that pure water is put into electrolytic cell, just extremely graphite,
Negative pole is platinum；Described pure water is, with natural mineral water or municipal tap water as water source, running water to be carried out more than three roads
Filter element filtering carries out purification process and obtains pure water.Above-described electrolytic process operating temperature is 50-80 DEG C, and operating current is
0.6-2.0 A/cm2。
Embodiment 4, making oral honeysuckle flower tea
The Camellia nitidissima leaf or flower dry cream that embodiment 1 is obtained add pure water and sucrose, every liter of water to add 1-3 grams of dry cream, sugarcane
Sugared 10-20 grams；Bottling after well mixed, sterilizing obtains anti-inflammation, clearing heat and detoxicating camellia chrysantha beverage.
Embodiment 5, making camellia nitidissima granules agent
Every 0.3 kilogram of addition, 1.0 kilograms of the soluble starch of Camellia nitidissima leaf or flower dry cream that embodiment 2 is obtained, oligosaccharide
0.3 kilogram, 0.5 kilogram of egg, after being well mixed, granulation obtains camellia nitidissima granules agent, for pachylosis, long on the face
The young man of whelk.
Embodiment 6, making Camellia nitidissima skin care item
Every 0.5 kilogram of addition, 0.4 kilogram of the starch of Camellia nitidissima leaf or flower dry cream that embodiment 3 is obtained, 0.3 kilogram of milk,
0.1 kilogram of trehalose, 0.4 kilogram of magnesium stearate, 0.8 kilogram of vaseline after being well mixed, as face cream, plays antibacterial
Antiinflammation.
Claims (5)
1. the extracting method of Camellia nitidissima active ingredient, is to roll into a ball water from plant Camellia nitidissima dry leaves or dried flower using alkaline small
The method of middle effective component extracting, it is characterised in that：Process is as follows：
1）Alkaline small group water is obtained first standby；Described alkaline small rolls into a ball pH 9-13；
2）Collection Camellia nitidissima leaf and flower, remove impurity removing, dry respectively, standby；
3）Dry Camellia nitidissima leaf and dried flower piece are smashed with pulverizer respectively,
4）It is put into respectively in the Camellia nitidissima leaf or flower smashed using the alkaline small group water of pH 9-13, per kilogram golden flower
Tealeaves or flower add 8-10 to rise alkaline small group water；Soaking temperature at 1-10 DEG C, time 3-5 hours, then in 10-20
DEG C temperature for the first time extract 3-5 hours, isolate extract solution and slag；The extract solution of Camellia nitidissima leaf or flower is individually deposited；
5）It is put into slag respectively using the alkaline small group water of pH 9-13；Per kilogram Camellia nitidissima leaf slag or flower slag are added
5-8 rises alkaline small group water；In 10-20 DEG C of temperature control second extraction 2-3 hours, alkaline extract solution and slag are isolated；Gold
The extract solution of jasmine tea leaf or flower is individually deposited；
6）It is put into slag respectively using the alkaline small group water of pH 9-13；Per kilogram Camellia nitidissima leaf slag or flower slag are added
3-5 rises alkaline small group water；Extracted 1-2 hours in 10-20 DEG C of temperature third time, isolate alkaline extract solution and slag；Gold
The extract solution of jasmine tea leaf or flower is individually deposited；
7）By alkaline extract solution respectively cross milipore filter, ultrafiltration membrane aperture between 0.05um to 1nm, usual molecular cut off scope
1000~300000, then with the ethanol elution of volumetric concentration 70-90%；Obtain the eluent of Camellia nitidissima leaf or flower；By
Ethanol and concentration are reclaimed, Camellia nitidissima leaf or flower dry cream is obtained；
8）Ethanol eluate low temperature is reclaimed into ethanol, alkaline extract solution is obtained, having containing Camellia nitidissima leaf or flower extracting liquid
Effect composition.
2. the extracting method of Camellia nitidissima active ingredient according to claim 1, it is characterised in that：Described pH 9-13's
The preparation of alkaline small group's water is that pure water is put into electrolytic cell, just extremely graphite, and negative pole is platinum；
In electrolysis, the chemical equation of the two poles of the earth generation is：
Negative electrode：2H2O+2e=2OH-+H2
Anode：2H2O-4e=4H++O2 。
3. the extracting method of Camellia nitidissima active ingredient according to claim 2, it is characterised in that：Described pure water Shi Yi cities
Political affairs running water is water source, and three road above filter element filterings are carried out to running water to be carried out at purification process acquisition pure water, or distillation
The distilled water obtained after reason.
4. the extracting method of Camellia nitidissima active ingredient according to claim 2, it is characterised in that：Described electrolytic process work
It is 50-80 DEG C to make temperature, and operating current is 0.6-2.0 A/cm2, then collect alkaline small group water from one side of negative electrode.
5. the alkaline extract solution that the extracting method of the described Camellia nitidissima active ingredient described in claim 1 is obtained is preparing food
Purposes in terms of product, cosmetics.
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| CN112386639A (en) * | 2019-08-14 | 2021-02-23 | 百岳特生物技术（上海）有限公司 | Application of golden camellia juice in preparing blue light resistant composition |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN107758628A (en) * | 2017-08-31 | 2018-03-06 | 华南农业大学 | A kind of nanometer selenium for preparing the method for nanometer selenium using camellia plant water extract and being prepared |
| CN107758628B (en) * | 2017-08-31 | 2020-10-09 | 华南农业大学 | Method for preparing nano-selenium by using camellia plant water extract and nano-selenium prepared by method |
| CN107912567A (en) * | 2017-12-28 | 2018-04-17 | 唐秀廷 | A kind of extracting method of golden camellia tea active material |
| CN108619321A (en) * | 2018-06-21 | 2018-10-09 | 大连大学 | Application of the Camellia nitidissima seed extract in alpha-glucosidase restrainer |
| CN112386639A (en) * | 2019-08-14 | 2021-02-23 | 百岳特生物技术（上海）有限公司 | Application of golden camellia juice in preparing blue light resistant composition |
| CN115429740A (en) * | 2022-08-18 | 2022-12-06 | 昆明康尔迅生物工程有限公司 | Preparation method and application of micro-molecular plant cell sap |
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Application publication date: 20170531 |