KR101448667B1 - Cosmetics for Containing Physiological, Anti-Oxidative and Anti-Microbial Activity Ingredients Separated from Urine and Thereof Method - Google Patents

Abstract

The physiological, antioxidative, and antimicrobial activity components separated from urine are collected by collecting the urine of 3-7 year old children, concentrating the urine dialyzed on the dialysis membrane with a freeze dryer, and then making powder to produce physiological, antioxidant, .
The physiological, antioxidant and antimicrobial active ingredients isolated from urine showed excellent DPPH radical scavenging ability, total flavonoid content, total polyphenol content and FRAP ability, and cosmetics containing this urinary component showed improvement of aging skin of women, It provides a useful effect for skin improvement such as erythema, melanin, yellow color change.
It can provide a useful effect in improving the face acne, especially in adolescents with a lot of acne.

Description

Technical Field [0001] The present invention relates to a cosmetic composition containing a physiological, antioxidant and antimicrobial active ingredient separated from urine, and a method for producing the same. [0002] Cosmetics for Containing Physiological, Anti-Oxidative and Anti-Microbial Activity Ingredients Separated from Urine and Thereof Method,

Physiology separated from urine, cosmetics containing antioxidative active ingredients, and methods for producing the same. More specifically, the urine collected from 3-7 year-old children is collected, and the urine dialyzed on the dialysis membrane is concentrated by a freeze dryer, and then powder is produced to obtain antioxidant and antimicrobial active ingredients. It is used to make lotions and lotions for women who want to improve their skin, or to suppress or improve acne.

Cosmetics containing the physiological, antioxidant and antimicrobial active ingredients of urine provide beneficial effects for improving the melamine, skin color, keratin, oil and moisture of women's face and neck, and also for the treatment of acne vulgaris And provides useful effects for improvement.

Urine (urine), also known as urine, is an accumulation of various waste products in the body as a result of the metabolism of the animal, and is stored in the bladder, and when it reaches the limit, it is discharged outside the body. Water constitutes more than 90% of the constituents of the urine, and as a next element, an adult man emits about 30 g per day. In addition, trace amounts of uric acid, amino acids, inorganic salts and contains. In addition to excretion of ammonia, which is the waste of nitrogen compounds, into the urea, urine has important physiological functions such as regulating the osmotic pressure of the body and controlling the water content.

Urine contains more than 25 enzymes such as leukocytes, red blood cells, epithelial cells, columnar cells, and other chemical substances such as type components, glucose, fructose, galactose, and carbohydrates, myoglobin, hemoglobin and fibrin degradation products such as galactose, amylase, peptidase, and urokinase , Hormones such as sex hormones, adrenaline, and endorphins, and chemical substances such as derectin, antineoplaston, 3-methylglyoxal, betaine turnic acid, and the like. These materials perform functions essential to our bodies such as antioxidant, anti-cancer, and tonic effects (Oser, 1965). In particular, directin has been reported to have the effect of reducing cancer cells to normal cells in 1996 in the Journal of the American Laboratory for Experimental Biology. An anti neoplaston was discovered by the American physician Bourgeinsky in the 1960s and has been steadily used by cancer patients as a peptidase agent that selectively destroys cancer cells without damaging normal cells. In addition, 3-methylglyoxal is known to destroy cancer cells (Kim, 2005).

The use of urine for the treatment of diseases, Dongbogobon (汤液 篇) 胡石 (秋 石) contains sex hormones, and the white blood of menstrual blood is effective in the brain hemorrhage is also used as a tincture . According to 鄕 药 集成 方, the 鄕 medicine 成 方 방 방 方 鄕 鄕 鄕 鄕 集 集............................... For the purpose of treatment of wounds, it is said that "when rubbing on the skin, the ruptured skin becomes soft, and when the worm is bitten by insects or dogs, it is wiped with hot urine". In Ishin's baseline gangmok (Ishinjin, 1996), the snake or a dog bites the affected part in warm urine. Recently, in China, cancer drug called CPA II, which is extracted from urine, has been produced (Hong Sung-kwon, 2008).

The most widely used of these was historically used as a cleanser for washing hands or washing clothes with urine in Rome, England, France, Korea, and in place of soap. In the early 18th century, French dentists used teeth whitening, periodontitis and toothpaste as a substitute for treating dental diseases. There are also many records of urine in Christian Bible, Buddhist scriptures, and ancient religious scriptures, among which the 3,000-year-old Koran scripture is one of the reasons why "not drinking urine is dying" . As such, urine has been used extensively in the prevention and treatment of diseases through its long history. In 1939, Dr. Adolf Friedrich Johann Butenandt was awarded the Nobel Prize for Chemistry for his discovery of sex hormones in the urine. A German doctor in the 1940s wrote a book on the experience of having a child with smallpox in his urine, and Dr. St. Jolz, a Nobel prize winner in the 1960s as a discoverer of vitamin C, (3-methyl glyoxal) from cancer cells and destroy cancer cells.

The results of this study are summarized as follows. First, the study on the effect of urine on the blood pressure and urine blood components of Kang Sun-hee (2002) (2006), and the effect of the method on hypertension and serum lipids. In addition, according to the efficacy of the lavage method introduced in the book of 333 people in the literature recommending the domestic medicine to the doctors, it is described that when the woman after the menopausal period drinks urine, freckles of the skin disappear and the skin becomes strong (Kang, ) And Kim, Ik-dal (1982), 333 (Ⅱ), Modern Medicine, Vol. 6, No. 594, are effective on yoga health and vigor. Also in this book, Lee Gyu-tae (1995) has introduced the contents of the use of urine in Korean myths and myths. In addition, urokinase extracted from urine is used as a representative treatment for cerebral hemorrhage, cerebral infarction, peripheral vascular occlusion, acute myocardial infarction, and pulmonary embolism. However, most of these prior arts have not been applied to skin care as a study on utilization of urine through diet.

Most of the conventional technologies related to urine have been used for the improvement of health through diet.

Urine contains various blood cells that have been metabolized in the body and physiologically active substances such as type components, carbohydrates, pigment substances, enzymes, sex hormones, moisture, urea, and inorganic salts. Therefore, .

The present invention compared the efficacy of collected day urine and 3-day-old mature urine, and compared with that day, it contained more mature yogurt protein and albumin at 3 days. Based on these results, we compared the results of freeze - dried dialysis day urine and dialysis day 3 days after dialysis to investigate the possibility of cosmetic raw materials for skin improvement. However, there are many proteins and albumin remaining after the dialysis process, and it is considered that various ingredients of urine which are not removed through the dialysis process improve the condition of the facial skin, so that the urine is supplied as a cosmetic raw material.

The physiological, antioxidant and antimicrobial active ingredients isolated from the urine of the present invention are excellent in DPPH radical scavenging ability, total flavonoid content, total polyphenol content and FRAP ability, and thus cosmetics containing the urinary component can improve the aging skin, It provides a beneficial effect on the improvement of erythema index, melanin index change, change in yellowness degree, skin color, keratin, oil, and moisture in each part of the neck. It provides a beneficial effect for the improvement of the face acne, especially for adolescents with a lot of acne.

1 shows a manufacturing process diagram of the present invention.
FIG. 2 shows skin changes of cosmetics prepared using active ingredients isolated from dialyzed urine (DU) and dialyzed urine (DAU) after 3 days of aging according to the present invention (The change of skin image DU & DAU group Before and after the experiment (A, before of the group, B, after of the group, C, before of the DAU group;

The present invention shows a method for separating physiological, antioxidant and antimicrobial active components from urine.

The present invention relates to a method for separating physiological, antioxidant and antimicrobial active components in urine, comprising the steps of putting urine into a dialysis membrane and dialyzing with water, and concentrating the active ingredient of the dialyzed urine with a freeze dryer.

The dialysis of urine can be carried out until the uric acid concentration reaches 30 (mg / dL) or less with any one selected from tap water, purified water or distilled water which is 1 to 500 times the urine weight per 1 part by weight of urine.

Dialysis can be carried out until the uric acid concentration reaches 10 to 30 (mg / dL) with any one selected from tap water, purified water or distilled water which is 1 to 500 times as much as urine per 1 part by weight of urine.

Dialysis can be carried out until the uric acid concentration reaches 1 to 30 (mg / dL) with any one selected from tap water, purified water or distilled water which is 1 to 500 times as much as urine per 1 part by weight of urine.

In the above, dialysis of urine can be carried out until the concentration of uric acid reaches 0.01 to 30 (mg / dL) with any one selected from tap water, purified water or distilled water which is 1 to 500 times as much as urine per 1 part by weight of urine.

The present invention includes physiological, antioxidant and antimicrobial active ingredients isolated from urine by the above-mentioned method.

In the above, physiological, antioxidant and antimicrobial active ingredients may have DPPH radical scavenging ability, flavonoid, polyphenol and ferric reducing antioxidant power (FRAP).

The present invention includes cosmetics containing physiological, antioxidant and antimicrobial active ingredients separated from urine by the above-mentioned method.

The cosmetics may be separated from urine and may contain one or more physiological, antioxidant and antimicrobial active ingredients selected from DPPH radical scavenging ability, flavonoid, polyphenol and FRAP (ferric reducing antioxidant power) ability.

The cosmetic may be any one selected from lotion, lotion, cream, ointment and pack.

The present invention includes acne remedies containing physiological, antioxidant and antimicrobial active ingredients separated from urine by the above-mentioned method.

Hereinafter, the present invention will be described in more detail.

The present invention relates to a method for the production of a physiological, antioxidant and / or antimicrobial agent in urine, comprising the steps of putting urine into a dialysis membrane and diluting it with purified water, tap water or distilled water to dialyze and concentrating the active ingredient of the dialyzed urine with a freeze- Lt; RTI ID = 0.0 > active ingredient.

Dialysis is carried out by using a semipermeable membrane to encapsulate the colloidal polymer solution and immersing it in a pure or large amount of solvent so that impurities mixed with low molecular weight or ion are diffused out of the membrane, . Semipermeable membranes or animal membranes are free to pass small molecules and ions, but not colloidal particles or polymers.

The dialysis effect of urine is aimed at eliminating components that can cause side effects such as excessive uric acid or ammonia contained in urine using the principle that the diffusion rate of colloidal particles or polymer is slower than the diffusion rate of solute. Therefore, dialysis of the urine will reduce the side effects of the skin.

The dialyzed urine may be used as it is, but if it is concentrated by using a freeze dryer, the effect is improved and the operation can be simplified.

In the above, the antioxidant active ingredient isolated from urine is collected from urine of 3-7 year-old children, and the urine dialyzed on the dialysis membrane is concentrated by a freeze dryer, and powder is prepared to obtain physiological, antioxidant and antimicrobial active ingredients. It is used to make lotions and lotions for women who want to improve their skin, or to suppress or improve acne.

The present invention includes physiological, antioxidant and / or antimicrobial active ingredients separated from urine by the above-mentioned method.

The physiological, antioxidant and / or antimicrobial active ingredients separated from urine by the above-mentioned methods of the present invention may contain components having DPPH radical scavenging ability, flavonoid, polyphenol and FRAP ability.

The present invention represents a cosmetic comprising physiological, antioxidant and / or antimicrobial active ingredients separated from urine by the above-mentioned method

The cosmetic may have DPPH radical scavenging ability, flavonoid, polyphenol and FRAP ability, including physiological, antioxidant and / or antimicrobial active ingredients separated from urine.

The cosmetics containing the physiological, antioxidant and / or antimicrobial active ingredients separated from the urine may be cosmetics selected from lotions, lotions, creams, ointments and packs.

The present invention represents an acne remedy comprising physiological, antioxidant and / or antimicrobial active ingredients separated from urine by the above-mentioned method

The acne remedy comprising physiological, antioxidant and / or antimicrobial active ingredients isolated from urine of the present invention can be formed into any one form selected from ointments, liquid agents and patches.

Hereinafter, the contents of the present invention will be described in detail with reference to experimental examples and examples. However, these are for the purpose of illustrating the present invention in more detail, and the scope of the present invention is not limited thereto.

≪ Experimental Example 1 > Dialysis of urine

In order to analyze the physiological, antioxidant and antimicrobial activities of the urine component and the components contained in the urine, the urine (U), the 3 day maturation (AU), the dialyzed day (DU) (DAU) was used.

Urine collection was provided by 50 male children between the ages of 3 and 7 at Y day care center in Wolgye-dong, Seoul, Korea in October 2011. The dialysis day (DU) and dialysis day 3 (DAU) MWCO 12,000-14,000 Da (Viskase, IL USA)] and one of tap water, purified water or distilled water having a volume of 1 to 500 times the volume of urine was flowed in a fluid manner to purify the impurities. In addition, water was exchanged every 1 to 8 hours when it was fixedly purified. The dialysis was completed until the concentration of uric acid in the sample became 30 mg / dL or less. The dialyzed urine was used as a sample in powder form using a freeze dryer (VFDPH-0050 Soitech, Korea) to concentrate the components of the sample.

<Component analysis of urine>

DAM-TSC reagent and phosphoric acid were used to measure the concentration of urea component, and a spectrophotometer was used. Other components were benzethonium for quantitative analysis of proteins, uricase (enzyme) for uric acid measurement, TIA for microalbumin and creatine, and Hitachi P module (Japan) was used for the instrument. Specific reagents required for each assay method were purchased from Roche (USA), and the contents of erythrocytes, leukocytes, and epithelial cells were observed with a microscope (Olympus TL4, Japan). The U concentration was the highest in U (group 1), 3 days (AU), 3 days (U), dialyzed day (DU) Followed by AU> DU> DAU. After the dialysis, it was confirmed that it was decreased about 5 times compared with that before dialysis and remained about 20%. The uric acid content was highest in aged AU, followed by U> DAU> DU. After dialysis, it was almost removed and remained 0.03 ~ 0.06%, and the content increased slightly after aging. The creatinine concentration was highest in U, followed by AU> DAU> DU and decreased by 10 times or more after dialysis. Protein and microalbumin showed the highest AU, followed by U> DAU> DU. These two components were not significantly different after dialysis and increased after fermentation (Table 1). As a result, contents of urea, uric acid, protein, albumin and creatine contained in each urine were found. After dialysis, the content of low-molecular urea (60.06), uric acid (168) and creatine (131.1) decreased significantly, whereas protein or albumin (16,000), a polymer, showed almost no change after dialysis.

Ingredients in urine (mg / dL) <Source: LeeWon Medical Foundation> division U AU DU DAU Protein 19.69 19.73 14.04 15.81 Uric Acid 60.2 61.49 0.02 0.06 Microalbumin 0.31 0.42 0.28 0.45 Creatine 79.79 63.99 0.49 0.73 Micro RBC 0-1 0-1 0-1 0-1 Micro WBC 0-1 0-1 0-1 0-1 Micro Epithelial Cell 0-1 0-1 0-1 0-1

<Abbreviation; U: daytime urine, AU: 3 day maturation urine, DU: dialysis day, DAU: 3 day maturation dialysis>

&Lt; Experimental Example 2 > Antioxidant activity measurement

1) Measurement of DPPH radical scavenging ability

The DPPH radical scavenging activity of 2,2-Diphenyl-1-picrylhydrazyl (DPPH) was purchased from Sigma-Aldrich (USA), and the DPPH radical scavenging activity was measured using 0.1 mL Was added 0.9 mL of 4.1 × 10 ^-5 M DPPH solution, and incubated at room temperature for 25 minutes. The absorbance at 517 nm was measured. The control group, Trolox, well known as an antioxidant, was used.

The radical scavenging ability of each sample was expressed as a percentage calculated by {(absorbance of no added group) / (absorbance of added group)} × 100 electron donating ability (EDA).

In this experiment, the percentage of free radical scavenging activity of U (day), AU (3 days of fermentation), DU (dialysis day), DAU (3 days of dialysis) and the comparative substance Trolox were shown.

U showed 32.5% of cleavage activity of Toroxl 0.5mM in control group, and 47.3% of AU showed cleavage activity of Toroxl 1mM or more. In addition, DA and DAU showed high activities of 105.2% and 108.6%, respectively. Among them, DAU showed the highest scavenging activity. These results suggest that the activity of urine aged 3 days is higher than that of urine on the day, and that urinary dialysis is more active than general urine. there was.

2) Total flavonoid content measurement

NaNO ₃ used for the determination of flavonoid content was ShoWa Chemical (Japan), AlCl ₃ Junsei (Japan) and NaOH (Duksan Chemical) were used. The total flavonoid content was determined by the method of D. Marinova (2005). 0.4 mL of distilled water was added to 0.1 mL of the sample, 0.03 mL of 5% NaNO ₃ and 0.06 mL of 10% AlCl ₃ were added, mixed well and left at room temperature for 10 minutes Respectively. Then 0.2 mL of 1 M NaOH solution and 0.2 mL of distilled water were added. 1 mL of the reaction-terminated solution was taken and the absorbance at 510 nm was measured. Standard curves were prepared by using Catechin.

In the present invention, the flavonoid content of U, AU, DU, and DAU was calculated from a standard curve using catechin as a standard substance. As a result, the content was the highest at AU 2.68 mg / mL, followed by U at 2.27 mg / 1.80 mg / mL of catechin contained more than 0.5 mM catechin, while DAU was 1.27 mg / mL, slightly lower than catechin 0.5 mM. From the above results, it was found that the flavonoid content of the yogurt aged 3 days was higher than that of the day, and did not decrease much after the dialysis compared to the phenol content.

3) Total polyphenol content measurement

Na ₂ CO ₃ used in the measurement of the total phenol content was purchased from Sigma-Aldrich (USA), and Folin-Ciocalteu reagent from Hayashi Pure Chemical Industries (Japan) was used. Total phenolic content was determined by the method of Gutfinger (1981).

0.5 mL of sample is mixed with 0.5 mL of 2% Na ₂ CO ₃ solution, reacted for 3 minutes, then 0.1 mL of 50% Folin-Ciocalteu reagent is added and left at room temperature for 30 minutes. 1 mL of this solution was taken and absorbance was measured at 750 nm. The total phenol content was determined using a standard curve using gallic acid as a standard reagent.

In the present invention, the total polyphenol content of U, AU, DU, and DAU was measured as 36.87 mg / mL of AU and 32.51 mg / mL of U, And DAU was 4.66mg / mL, which was similar to that of 5mM of gallic acid. And DU had the lowest polyphenol content of 2.72 mg / mL. As a result, the polyphenol content of the urine was slightly higher than that of the day, and the dialyzed urine was also the same.

4) Ferric reducing antioxidant power (FRAP) measurement

The products of K ₃ [Fe (CN) ₆ ] and FeCl ₃ ㅇ 6H ₂ O used in FRAP measurement were Samchon Chemical (Korea) and C ₂ HCl ₃ O ₂ were used in Duksan Chemical (Korea) , Catechin, and gallic acid were purchased from Sigma-Aldrich (USA). Using a Microplate reader (Synergy, Biotek) instrument. To 0.1 mL of phosphate buffer (0.2M, pH 6.6), 0.1 mL of sample and 0.1 mL of 1% (w / v) K ₃ [Fe (CN) ₆ ] solution were added and the mixture was reacted at 50 ° C for 20 minutes , 10% (w / v) solution C ₂ HCl ₃ O _{2 (} 0.1 mL) and centrifuged at 10,000 × g for 10 minutes. 0.5 mL of distilled water was added to 0.5 mL of the supernatant obtained by centrifugation, 0.05 mL of a 0.1% FeCl ₃ O 6 H ₂ O solution was added, and the absorbance at 700 nm was measured. The reducing power was calculated as Trolox equivalent antioxidant capacity (TEAC) by standard curve (0-1000 μM) using Trolox as a standard.

In the present invention, the antioxidative activity of U, AU, DU, and DAU was compared with Trolox as a reference material. As a result, AU showed the highest reducing power of 3.37 μ mol / L and U showed 3.00 μ mol / L, AU and U showed higher antioxidant activity than Trolox 1mM. The dialyzed DAU was 2.11 ĩmol / L, which was higher than that of the control group, and DU was 0.17 ĩmol / L.

In the present invention, the antioxidative activity of DPPH radical was observed in all groups, and DAU was the highest. It was also found that the activity after dialysis was higher than that before dialysis, and that the ability to erase more than twice was increased after aging. It is presumed that this result is obtained during the dialysis process and the aging process due to the presence of a compound having a stereostructure suitable for reacting with DPPH among various components of urine.

&Lt; Example 1 > Preliminary experiment for skin application

Sixteen students in 20 college students living in Incheon were selected for experimental group 1 using general urine (U) and experimental group 2 using dialysis urine (DU). The skin application agent was a lotion type and diluted with 3% of alcohol in each urine, and then stored in the refrigerator twice a day in the morning and evening. After 2 weeks of use, questionnaires were made on the items of skin erythema, softness, satisfaction, and adverse reaction and compared with the mean value scored.

The results of the preliminary experiment showed that the use of the skin application agent applied with the general urine (U group) and the dialysis urine (DU group) for 2 weeks resulted in a decrease in skin erythema, softness, satisfaction, Table 2 shows the results.

In the first group, the total average score was 10 points, while the DU group using dialysis was 14 points higher. In addition, DU group did not respond to other factors, whereas U group described erythema and rash. These results suggest that both U and DU patients had a soft feeling on the skin, whereas the overall satisfaction was higher in the DU group than in the U group after the dialysis treatment. In addition, DU group showed more stability in other reactions than U group, and it was found that dialysis treatment for improvement of skin in the future can be used as a raw material of cosmetic without being overstated.

Two weeks after two weeks skin changes Item Erythema soft satisfaction system Etc U 3 4 3 10 Erythema, rash DU 5 4 5 14 none

Abbreviations: U, Urine; DU, dialysis urine

&Lt; Example 2 > Effect on the improvement of aging skin of urine

The subjects and period of the study were 30-60 years old women living in Seoul and Gyeonggi-do. We selected 30 patients who had problems or bad skin because of their skin problems. They were usually hospitalized, The subjects were divided into two groups: those who did not take the health food, the group that was dialyzed with DU, the group that was dialyzed with DAU that was 3 days old, and the control group (with distilled water). The study period was conducted from November 7, 2011 to January 3, 2012 for 8 weeks. In the present invention, lotion and lotion containing yoghurt were prepared and given to the DU group and the DAU group every 2 weeks, and used twice a day in the morning and evening, and the control group was prepared containing distilled water in the urine. In addition, the subjects were not allowed to use other ingredients for skin improvement such as medicines or functional cosmetics during the study period, and medical procedures, excessive drinking or smoking were prohibited. In order to investigate the skin improvement effect, the skin lotion and the lotion provided by each group were used. Before and after 8 weeks of use, the erythema index, melanin index, skin color, keratin content index, Water, pigment, water content, and pH were measured. For accurate results in this study, skin was cleaned with the same cleanser and measured 30 minutes later. The material is the same as the one used in the Experimental Example. The sample is concentrated three times and two kinds of lotion (lotion) are mainly composed of yogurt and lotion.

<Production of lotion>

The composition ratios of the lotion containing the urine used in the present invention are shown in Table 3. (B) was added to the mixture (A), (C) was mixed at room temperature, and the homomixer was stirred at 3,000 rpm for 10 minutes. The manufactured lotion was distributed to clinical subjects and stored in a refrigerator at 4 ° C or below.

Compounding ratio of urine-containing skin toner (lotion) (mL) ingredient Control
C   Dialysis
DU Three days of dialysis.
DAU A Distilled water   Up to
100 mL     - -  Yo -   Up to
 100 mL -  Aging - - Up to
100 mL B Hyaluronic acid One  GSE 0.3 C Lemon oil 0.5

<Lotion manufacturing>

Lotion formulations used in this experiment were prepared according to Table 4 below. The phase (A) and the phase (B) were heated to 75 ° C and completely dissolved. The phase (A) was added to the phase (B) and emulsified by stirring the mixture at 3,000 rpm for 15 minutes. Then, (C) phase was added, and the mixture was stirred at 3,000 rpm for 5 minutes, and the lotion formulation was prepared and used. The prepared lotion was distributed to the subjects of the clinical experiment and stored in a refrigerator at 4 ° C or below.

Compounding ratio (mL) of yore-containing emulsion (lotion) ingredient  Control group
C Dialysis on the same day.
 DU Three days of dialysis.
DAU A  Distilled water   Up to
 100 mL    -     -  Yo     -  Up to
100 mL     - Aging     -    -   Up to
 100 mL B Jojoba oil                     10 Shea butter                      5 Emulsified wax                     1.5 C Hyaluronic acid                      One  GSE                     0.3 Lemon oil                     0.5

<Forehead, left-handed, right-handed,

In order to investigate the effects of urinary components on the changes of the erythema index in 30 women aged 40-60 years,

There was no statistically significant difference in the DU group in the forehead region. On the other hand, in the DAU group, there was a statistically significant difference (p <0.05) in reducing the erythema level of the forehead by decreasing from 333.6 in the pre-injury to 289.4 in the post-

In the left hand group, the control group increased 16% from 277.3 to 323.0 (p <0.05). There was a significant difference (p <0.01) in the DU group by 13% from 361.0 to 312.5. In the DAU group, there was a significant difference (p <0.05) from the pre-325.7 to post-285.8, 12% decrease.

In the right ball, the control group showed a 10% increase from the pre-292.4 to the post-321.9, but not statistically significant difference. On the other hand, in the DU group, the erythema level decreased significantly from 346.0 before to 301.8 after the injury. In the DAU group, there was a significant difference (p <0.05).

There was no statistically significant difference in the jaw area. On the other hand, in the DU group, there was a significant difference (p <0.05) between the pre-461 and post-mortem 419.1 (p <0.05) 0.05).

The mean total erythema index of the control group was significantly increased by 6% from 324.7 to 343.9 (p <0.05). In the DU group, there was a significant difference (p <0.01) between the pre - 373.9 and post - mortem 337.45 (p <0.01) and there was a significant difference between the DAU group and the post - mortem group from 357.98 to 316.18.

These results suggest that cosmetics containing yogurt have a positive effect on the improvement of erythema of women 40-60 years old.

<Changes in erythema index by group>

Changes in the erythema index of each group were found to be different in the forehead, the left ball, the right ball, the jaw, and the overall change.

In the forehead, the decrease was 5.5 in the control group, 11.2 in the DU group, and 44.2 in the DAU group. The DAU group showed the highest reduction of the forehead erythema index in the order of the DU group and the control group (p <0.05).

In the left-hand group, there was a 45.7 increase in the control group, a 48.5 decrease in the DU group, and a significant decrease in the DAU group (39.9), indicating a statistically significant difference between the DU group and the DAU group (p <0.001).

In the right-hand group, the increase was 29.5 in the control group, 44.2 in the DU group, and 39.8 in the DAU group (p <0.05).

In the jaws, the control group showed an increase of 7.2, whereas the DU and DAU groups showed a decrease of 41.9 and 43.3, respectively (p <0.05).

In addition, there was a significant difference (p <0.001) in the DAU group compared with the DAU group, with a decrease of 36.9 and a decrease of 41.8, respectively.

&Lt; Example 3 > Change of facial skin melanin index

In order to evaluate the effect of urine components on the facial skin melanin index of the aged skin, a total of 30 subjects of 10 control group, 10 DU group, and 10 DAU group were administered with the lotion and lotion of Example 1 in the morning and evening 1 day The skin melanin index was measured before and after the experiment.

<Change of melanin index by site>

As a result of this process and various factors, the melanin produced in the skin was increased 3% from 177.9 in the control group to 183.4 in the control group after the use of cosmetics containing the urine, but there was no statistically significant difference . On the other hand, in the DU group, the DAU group showed a decrease of 9% from 164.5 to 150.3 (p <0.05). In the DAU group, the DAU group showed a decrease of 11% Effective.

In the case of the left ball, the control group increased by 4% from 142.9 to 148.6, but it was not statistically significant. In the DU group, it decreased by 13% from 164.3 to 142.3 (p <0.05). In the DAU group, there was a 22% significant decrease from pre-154.4 to post-mortem 120.6 (p <0.01).

In the case of the right ball, the control group increased by 13% from 128.8 to 145.9, but it was not statistically significant. On the other hand, in the DU group, the melanin level decreased by 11% (p <0.05) from the pre-156.5 to post-mortem level of 138.6. In the DAU group, the melanin level was significantly decreased by 24% (p <0.01).

In the control group, 7% increase from pre-191.9 to post-205.6, but not statistically significant difference, whereas in the DU group, it decreased from pre-204.2 to post-179.6 by 12% (p <0.001) There was a significant difference (p <0.01) by 14% from 188.8 to 163.3.

The DAU group decreased 17% from the pre-165.40 to the post-death rate of 137.03 from the pre-172.38 to the post-152.70 (11%) decrease in the DU group (P <0.01), respectively. These results suggest that cosmetics containing urine in the order of DAU and DU decrease the melanin index.

<Changes in Melanin Index by Group>

As a result of analysis of variance of melanin index and total average of the groups, the control group was increased by 5.5 in post-experiment compared to pre-experiment, 14.2 in the DU group and 17.7 in the DAU group, and the DU and DAU groups decreased the melanin levels statistically (P <0.01), respectively.

In the control group of the left ball, the DAU group was significantly decreased by 33.8 (p <0.01).

In the right-hand ball, 17.1% decrease was observed in the DU group and 36.5% in the DAU group, compared with 17.1% increase in the control group (p <0.01).

In the case of the jaw, there was a significant difference (p <0.01) in the DA group compared to the DA group.

The mean total results were significantly (p <0.001) higher in the control group than in the experimental group (10.5), in the DU group (19.68) and in the DAU group (28.38).

As a result, it was confirmed that DAU group was the most effective for reducing the melanin index (p <0.001).

<Change in yellowness (SCE b *) by site>

There was no statistically significant difference between the control group and the DU group before and after the experiment in the forehead region, but there was a difference in the DAU group .

In the case of forehead, there was a 10% increase from pre - 21.87 to post - 23.97 (p <0.05) and a significant increase of 9% from right 21.59 to post 23.54.

Also, the jaw was significantly increased 19.0% from 20.03 to 23.87 (p <0.01).

In the overall average, the DAU group increased by 11% from 21.34 to 23.64, indicating a statistically significant effect (p <0.01).

&Lt; Example 4 > The effect of urine on acne skin improvement

<Subjects and Methods>

Prior to this study, research subjects such as questionnaires, methods of use, and skin measurements were explained. Among those who agreed to participate in the study, those who had experience of illness or past disease or who were taking medicines or hormones Were excluded from the study. The selected subjects were 30 college students in their 20s and currently have problems with acne skin. They were classified according to the questionnaire survey and acne grade evaluation before the experiment. The control group consisted of 10 control subjects (group C) Ten patients (DU group) and 10 patients (DAU group) were matured three days after dialysis.

Acne rating is a method of evaluating the grade after visual observation according to the condition of acne in four stages of I-VI. The study was based on Donald Pillsbury's acne classification table. The study period was 8 weeks from November 7, 2011 to January 3, 2012.

<Measurement of facial skin acne grade evaluation step>

Dr. The four acne classification tables of Donald Phillsbury were used to classify the skin according to the skin condition. The evaluation is divided into the non-inflammatory acne and the inflammatory acne according to the five types of acne on the face (left forehead, right side of the cheek and nose area), blac head, white head, papule, pustule, nodule, cyst and scar , The number of which is visible to the naked eye. Grade I (the weakest grade, black head, white head complex, approximately 10 facial skin, scar and inflammation are rarely seen ), Grade Ⅱ (there are a lot of whiteheads, including 10 to 25 facial papules including mild scars and inflammation sites, acne less than 5 mm in diameter), Grade Ⅲ (more than 25 Pistula, pustule, pustule, pustule, pustule, pustule, pustule, pustule, white head, black head, Was evaluated in four stages of acne condition says that the site exists). Measurements were taken by the same person twice before and 8 weeks after use.

<Acne rating change>

In order to evaluate the effect of the lotion and lotion applied on the acne skin on the change of the acne grade, 30 students in their twenties with acne were measured before and after using acne grade. Improvement in acne grade means improvement in the number of inflammatory acne. The results of this study are as follows: (1) In the case of acne vulgaris, there is no evidence of acne vulgaris. According to the improvement and progress of acne skin, before and after cosmetic use of the subject of acne, in the control group, it was changed from Grade Ⅲ to Grade Ⅱ by 10% increase from pre-2.7 to post-3, but this did not show statistically significant difference. On the other hand, in the case of DU, the DA group was improved from Grade Ⅱ to Grade Ⅰ (p <0.001) and decreased from Grade Ⅱ to Grade Ⅰ by decreasing 61% from 2.9 to 1.8 The group and DAU group were found to be effective in lowering acne grade (p <0.001).

<Changes in acne rating by group>

As a result of comparing the mean difference between the experimental and control groups after 8 weeks, the DA and UU groups showed a significant decrease in the DU and DAU groups, (P <0.001), respectively. These results suggest that urine is effective in lowering acne grade and that the effective comparison between DU and DAU group is insignificant.

<Acne rating change>

As a result of acne grade measurement, the control group increased by 10% and changed from Grade Ⅲ to Grade Ⅱ, but this did not show any statistically significant difference. On the other hand, in the case of the DU group, there was a 68% (P = 0.000) decrease from the prior 2.7 to the posterior 1.6, and the Grade Ⅱ to Grade Ⅰ significantly decreased, and the DAU group decreased from 61% (P = 0.000) Grade I, and the DU and DAU groups were found to be effective in lowering the grade of acne (p <0.001). Compared with the control group, the DU group and the DAU group showed a significant decrease in the acuity grade of the DU group and the DAU group by 1.1 level, respectively (p <0.001).

Although the present invention has been described with reference to the preferred embodiments thereof, it will be apparent to those skilled in the art that various changes and modifications may be made without departing from the spirit and scope of the present invention as set forth in the following claims It will be understood that the invention may be modified and varied without departing from the scope of the invention.

The physiological, antioxidant and antimicrobial active ingredients isolated from the urine of the present invention are excellent in DPPH radical scavenging activity, total flavonoid content, total polyphenol content and FRAP ability, and these cosmetic compositions containing urinary components are useful for improving aging skin of women, Of the skin, such as erythema, melanin, change in yellowness. Especially, it is industrially applicable because it provides a useful effect for improvement of face acne in adolescents who have a lot of acne in adolescence.

Claims (6)

Collecting the collected urine for 3 days,
Placing the aged urine in a dialysis membrane and dialyzing with water, and
And concentrating the active ingredient of the dialyzed urine with a lyophilizer, wherein the active ingredient contains a flavonoid or polyphenol. The urine of claim 1, wherein the dialysis is performed by dialysis until the uric acid concentration reaches 30 (mg / dl) or less with any one selected from tap water, purified water or distilled water which is 1 to 500 times the urine weight A method for separating physiological and antioxidant active ingredients. delete delete delete delete

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