CN103463627A - Oral care composition containing heat-resistant superoxide dismutase and growth factors - Google Patents
Oral care composition containing heat-resistant superoxide dismutase and growth factors Download PDFInfo
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- CN103463627A CN103463627A CN2013104138764A CN201310413876A CN103463627A CN 103463627 A CN103463627 A CN 103463627A CN 2013104138764 A CN2013104138764 A CN 2013104138764A CN 201310413876 A CN201310413876 A CN 201310413876A CN 103463627 A CN103463627 A CN 103463627A
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Abstract
The invention discloses an oral care composition having an antibacterial and anti-inflammatory function and a prevention and restoration effect. The effective components of the oral care composition comprise heat-resistant superoxide dismutase, epidermal growth factors and liposome. The heat-resistant superoxide dismutase has stability higher than that of ordinary superoxide dismutase and has the function of improving the gingival bleeding and red and swollen symptoms caused by a variety of oral problems; the epidermal growth factors have the functions of promoting wound healing of skin and mucosa, diminishing inflammation, relieving pain and preventing ulcer; the unilamelar liposome wraps the heat-resistant superoxide dismutase and the epidermal growth factors to ensure more durable biological activity and has targeting and transdermal absorption properties.
Description
Technical field
The present invention relates to field of oral care, be specifically related to the oral care composition of a kind of liposome heat-resistant superoxide dismutase and epithelical cell growth factor.
Background technology
Superoxide dismutase (Superoxide dismutase), be called for short SOD; Be the protein that is rich in metal ion, extensively be present in animal, plant, microbial cell or organelle.By its containing metal prothetic group difference, can be divided three classes; The first kind is called for short Cu-Zn-SOD containing Cu, Zn(), mainly be present in eukaryotic cytoplasm; Equations of The Second Kind is containing Mn(Mn-SOD), be present in eukaryotic mitochondrion and prokaryotic cell; The 3rd class is containing Fe(Fe-SOD), only be present in prokaryote.Mc Cord and Fridovich purification in 1969 enzymatic property and the main biological function of having illustrated the Sanguis Bovis seu Bubali erythrocyte superoxide dismutase.Superoxide dismutase dissimilar, separate sources all has an extremely important biological function, i.e. catalysis superoxide radical generation dismutation reaction is known as the first line of defence and the most important antioxidase of superoxide radical.Experiment showed, that superoxide dismutase has Promote cell's growth, treatment scytitis, antidotal effect.
Epithelical cell growth factor (Epidermai Growth Factor), be called for short EGF; Being the biologically active polypeptide be comprised of 53 aminoacid in a kind of Skin Cell, body fluid, kidney and cerebral tissue that extensively is present in the people, is a kind of bioactive substance with physiological function, has very strong biological effect.In human body, the epithelical cell growth factor of denier just can play very large biological action, can promote propagation and the growth of skin tissue cell, makes newborn young cell replace rapidly aging and dead cell.Be regularly Skin Cell supplementary table skin cell growth factor, just can continue to promote synthesizing of cell, the vigorous metabolism of maintenance epidermis cell, promote in addition skin and mucosa wound healing, anti-inflammatory analgetic, prevent and treat the effect of ulcer.
But find under study for action, there is following problem in superoxide dismutase in the mouth care application facet: at first, superoxide dismutase enters its active half-life shorter (only several hours) after cell; Moreover the superoxide dismutase biological activity may be subject to the impact of other substrate in oral-cavity article and be unstable.Epithelical cell growth factor also belongs to the biotechnology preparation, has special biological activity, tend to oral-cavity article in chemical substance react and lose activity.Simultaneously, epithelical cell growth factor, superoxide dismutase are higher than 37 ℃ or all can inactivation under the pH condition beyond neutrality.Various biological preparation have different application points and receptor, if fill a prescription improper or improper use, and DeGrain not only, and also dangerous.
The Fe-SOD that has research worker to extract from a kind of extreme Thermophilic Bacteria (Aquifex pyrophilus), make heat-resistant superoxide dismutase, solved the central inadequate problem of stability of common superoxide dismutase application.
Adopt liposome also can improve the stability of biological preparation simultaneously.The application of liposome in cosmetics is many, mainly comprises two aspects: one, liposome is avoided bioactive substance to interact or biodegradation occurs as the carrier of functional components; Two, the phospholipid in liposome membrane and unsaturated fatty acid itself have good moisturizing and skin care effect.Particularly it forms similar lipoid to skin to liposome, and liposome as formed as sphingomyelin and keratodermatitis lipid have high similarity, can increase medicine the accumulating of local skin, and strengthens the skin targeting, and can the sustained release medicine; There is the enhancing medicine and enter the effect in horny layer and epidermis lipoid.
It is the topical for skin in essence that liposome is applied in cosmetics: when with skin, for targeting moiety, designing liposome, be retained in skin with should making the medicine maximum, enter as little as possible blood circulation, the effectiveness composition acts on the horny layer of skin surface or enters epidermis or corium.Be that in cosmetics, liposome and other functional components will be realized transdermal penetration and avoid seeing through skin simultaneously entering blood circulation, cause toxic and side effects.At present, the research of liposome percutaneous dosing shows: the liposome that the liposome particle diameter is greater than 1000nm mainly is stranded in horny layer; The liposome of 600nm-1000nm can be penetrated into epidermal area and skin corium; The liposome that is less than 200nm can directly enter systemic circulatory system, causes toxic and side effects.Therefore, we to take the method for preparing unilamelar liposome when selecting preparation method be basis.
At present, superoxide dismutase or epithelical cell growth factor are used in separately to the existing correlational study report of oral care product; And heat-resistant superoxide dismutase does not have related application research report, heat-resistant superoxide dismutase and epithelical cell growth factor are applied to oral care product by liposome does not have the correlational study report yet.
Summary of the invention
The object of the invention is to overcome the deficiencies in the prior art, provide a kind of good stability, targeting, sustained-release and controlled release is effective and to the oral care composition of oral inflammation, the effective liposome heat-resistant superoxide dismutase of ulcer prevention and epithelical cell growth factor.
Another object of the present invention is to provide the application of above-mentioned oral care composition in oral care product.
Above-mentioned purpose of the present invention is achieved by following scheme:
A kind of oral care composition, described oral care composition by liposome with unilamelar liposome wrapped heat-resistant superoxide dismutase, epithelical cell growth factor and form; Described liposome mean diameter is between 600-1000nm; Described heat-resistant superoxide dismutase accounts for the 0.1-10.0% of composition weight, and concentration is 50-500U/ml; Described epithelical cell growth factor accounts for the 0.1-10.0% of composition weight, and concentration is 0.1-100mg/ml; Described liposome accounts for the 80%-99.8% of composition weight, and mean diameter is between 20-5000nm.
Oral care composition of the present invention, described heat-resistant superoxide dismutase is the Fe-SOD lyophilized powder extracted from a kind of extreme Thermophilic Bacteria (Aquifex pyrophilus).
Oral care composition of the present invention, its molecular weight of heat-resistant superoxide dismutase wherein is between 38700 to 40000, and concentration is between 100-250U/ml.
Oral care composition of the present invention, described epithelical cell growth factor is at least one in lyophilized powder, stock solution form, concentration is between 0.2-20mg/ml.
Oral care composition of the present invention, described liposome is by a phosphate and hydrophilic radical and two lipophilic groups that longer alkyl forms that quaternary ammonium salt base forms.
Oral care composition of the present invention, wherein liposome at least comprises one or more compositions in lecithin, cholesterol, ceramide, sphingomyelins, between mean diameter 300-1300nm.
Oral care composition of the present invention, its isoelectric point, IP is between 5-7.
Oral care composition of the present invention can be used in the oral care products such as toothpaste, collutory, gel, powder, confectionery and lozenge.It is 0.01%-5% that its content accounts for percentage by weight in oral care product.
Compared with prior art, the present invention has following beneficial effect:
1. adopt heat-resistant superoxide dismutase in oral care implement compositions provided by the invention, in the stability of production application and product shelf life higher than common superoxide dismutase.
2. oral care implement compositions provided by the invention adopts unilamelar liposome parcel heat-resistant superoxide dismutase and epithelical cell growth factor, and liposome provides protection to two kinds of biological preparation, has improved the stability of compositions.
3. because the horny layer phospholipid layer content of human body skin is less, in basal layer, phospholipid content is the highest, oral care implement compositions provided by the invention is more easily dissolved and is slowly discharged by phospholipid in basal layer, allows heat-resistant superoxide dismutase and epithelical cell growth factor be absorbed by the skin.
4. oral care implement compositions provided by the invention adopts heat-resistant superoxide dismutase and the composite use of epithelical cell growth factor, plays the effect of Synergistic, has enlarged the scope of application in oral-cavity article.
5. the compositions scope of application of oral care implement provided by the invention is extensive, is applicable to the multiple oral diseases such as periodontitis, gingivitis, gingival hemorrhage.
The specific embodiment
Heat-resistant superoxide dismutase of the present invention is the Fe-SOD extracted by a kind of extreme Thermophilic Bacteria (Aquifex pyrophilus), and its molecular weight is between 38700 to 40000.Have following two features: (1) shows good heat stability under hot conditions, at 80 ℃, processes 1～2 hour, and enzyme activity remains on more than 95%, at 100 ℃, processes 1～2 hour, and enzyme activity remains on more than 40%; (2) keep the enzyme activity more than 90% in the pH4-11 scope.The Technology of fermentation high temperature-resistant hyperoxide dismutase has obtained national inventing patent (Zl200510005207.9).
Liposome of the present invention can be in lecithin, cholesterol, ceramide, sphingomyelins one or more form.Can guarantee stability, biocompatibility, Transdermal absorption and the slow release controlled-release effect of heat-resistant superoxide dismutase and epithelical cell growth factor.
Liposome of the present invention can be by preparing with dehydration rehydration method or prefabricated liposome method.This preparation method is disclosed by prior art, and the envelop rate additive method of comparing is the highest, reaches 65-80%.
Compositions of the present invention adopts the unilamelar liposome parcel, and particle diameter is between 600-1000nm.According to the transdermal very easily of the liposome below research diameter 600nm, the above liposome of diameter 1000nm easily rests in horny layer.This liposome structure can effectively act on wrappage in skin layer, embodies its targeting.
The accompanying drawing explanation
Fig. 1 is that patients with periodontal disease is used the LDH of embodiment 2 dentifrice compositions front and back to compare (n=40).
Below in conjunction with specific embodiment, the present invention is done further and describes, but specific embodiment is not done any restriction to the present invention.
Embodiment 1
Oral care composition of the present invention is comprised of each component of following mass fraction:
The mean diameter of above-mentioned oral care composition is 600-800nm.
Above-mentioned oral care composition heat-resistant superoxide dismutase, epithelical cell growth factor, lecithin, cholesterol, sphingomyelins are commercially available sterling.
Above-mentioned oral care composition preparation method, its concrete steps are as follows:
Heat-resistant superoxide dismutase, epithelical cell growth factor, lecithin, cholesterol, sphingomyelins are passed through to heating and melting or use organic solvent dissolution, make lipid soln.
By fluid bed, by above-mentioned liposome solutions Direct spraying and the proppant be suspended in the middle of fluid bed, the volatilization organic solvent, obtain heat-resistant superoxide dismutase/epithelical cell growth factor liposome.
Organic solvent of the present invention comprises dichloromethane, chloroform, ether and ethanol.
Proppant of the present invention is selected from a kind of in mannitol, glucose, sorbitol, sucrose, lactose, trehalose, sodium chloride and polyvinylpyrrolidone.
A kind of dentifrice composition of the oral care composition that contains liposome heat-resistant superoxide dismutase and epithelical cell growth factor, its component proportion is as follows:
The concrete preparation method step of above-mentioned dentifrice composition is as follows:
Saccharin sodium, sodium benzoate is water-soluble, make water; After adding Sorbitol and PEG400, mix homogeneously.
A, B, C, D compositions in silicon dioxide, sodium carboxymethyl cellulose, laurilsulfate sodium, embodiment 1 are dispersed in respectively in the liquid phase of step 1, and high-speed stirred is also fully mixed, and obtains dentifrice composition after vacuum outgas.
Embodiment 3
A kind of collutory compositions of the oral care composition that contains liposome heat-resistant superoxide dismutase and epithelical cell growth factor, its component proportion is as follows:
The concrete preparation method of above-mentioned collutory compositions is as follows:
Saccharin sodium, sodium benzoate is soluble in water, add the poloxamer188 ageing to spend the night.
A, B, C, D compositions in Sorbitol, spice and embodiment 1 are added respectively in the liquid phase of step 1, and the rear filtration that stirs obtains the collutory compositions.
The stability experiment of the oral care composition of embodiment 4 liposome heat-resistant superoxide dismutases and epithelical cell growth factor in the oral care product application
By the toothpaste A compositions of embodiment 2 and the independent superoxide dismutase that adds, the dentifrice composition of epithelical cell growth factor is in 40 ℃ of temperature, airtight placement under relative humidity 75% condition, in placing rear 0, 1, 2, March, measure respectively the content of heat-resistant superoxide dismutase and epithelical cell growth factor in liposome by the ELISA method, during with 0 month in liposome heat-resistant superoxide dismutase content and epithelical cell growth factor content be respectively 100%, the content of other each time heat-resistant superoxide dismutase and epithelical cell growth factor is made comparisons with it respectively, observe the time dependent situation of content of heat-resistant superoxide dismutase and epithelical cell growth factor.
The superoxide dismutase change percentage in arid
The epithelical cell growth factor change percentage in arid
Result shows, application is higher with stability in tooth paste product separately than common superoxide dismutase and epithelical cell growth factor for the oral care composition of liposome heat-resistant superoxide dismutase and epithelical cell growth factor.
The oral care composition result of use experiment of embodiment 5 liposome heat-resistant superoxide dismutases and epithelical cell growth factor
Periodontal disease is that a kind of sickness rate is very high, the chronic disease of the dental tissue of serious harm human health.Clinical symptoms is red swelling of gingiva, hemorrhage and odontoseisis, and severe patient even develops into loss of tooth.Tooth paste product containing heat-resistant superoxide dismutase and epithelical cell growth factor can act directly on gingiva tissue, is conducive to defend the damage of superoxides too much in inflammation tissue to cell, makes the controlled and disappearance of inflammation, repairs the damaged cell tissue simultaneously.Reach the purpose of prevention and alleviation periodontal disease.
Material:
Patients with periodontal disease 40 examples, male's 20 examples, women's 20 examples, in age 20-60 year, 32 years old mean age, at least do not carry out periodontal treatment half a year; Negative control group 40 examples, male's 20 examples, women's 20 examples, age 20-60 year, 33 years old mean age, through clinical examination all without systemic disease, in oral cavity all without the acute and chronic focus.
Toothpaste A compositions in embodiment 2
Method:
Laboratory is differentiated: saliva lactic acid dehydrogenase (LDH) Biochemical Indexes.
Lactic acid dehydrogenase (LDH) is a kind of very stable Cytoplasm enzyme, is present in Normocellular kytoplasm, once damaged membrane, lactic acid dehydrogenase is released to extracellular; Lactic dehydrogenase enzyme catalysis lactic acid forms pyruvate and INT(tetrazolium salt) reaction changes into red first Za compound, can be detected by microplate reader.The amount that color forms is directly proportional to the number of cell lysis.Apply the absorption value data that a dull and stereotyped reader in 96 holes is collected visible wavelength.This analysis can be used for measuring the integrity of cell membrane in cell-mediated cytotoxicity analysis, and target cell, by effector lymphocyte's cracking, can judge the degree that cell is impaired in this case.Have report can be used as the biochemical indicator of monitoring periodontal tissue destructiveness, in Saliva with Periodontitis, lactic acid dehydrogenase obviously raises.
The tester respectively collects saliva 1ml, censorship in before and after two weeks, one month, two months with using embodiment 2 toothpaste.
Test result:
Normal population is lactic acid dehydrogenase average out to 268 before using embodiment 2 dentifrice compositions, and minimum is 47, is up to 580.
The periodontal patient is lactic acid dehydrogenase average out to 668 before being to use embodiment 2 dentifrice compositions, and minimum is 58, is up to 1789.
Clinical manifestation:
Normal population is after use, and 40 routine negative control person oral cavities are without significant change.
The periodontal patient is using embodiment 2 dentifrice compositions after two weeks, and 40 routine patients with periodontal diseases show as gingival hemorrhage to be reduced; After one month, 37 routine patients with periodontal diseases have no longer included the gingival hemorrhage symptom, the gingival swelling and pain number of times reduces, clinical effective rate 92.5%, inefficiency 7.5%.
Claims (8)
1. an oral care composition is characterized in that: described oral care composition by liposome with unilamelar liposome wrapped heat-resistant superoxide dismutase, epithelical cell growth factor and form; Described liposome mean diameter is between 600-1000nm; Described heat-resistant superoxide dismutase accounts for the 0.1-10.0% of composition weight, and concentration is 50-500U/ml; Described epithelical cell growth factor accounts for the 0.1-10.0% of composition weight, and concentration is 0.1-100mg/ml; Described liposome accounts for the 80%-99.8% of composition weight, and mean diameter is between 20-5000nm.
2. according to the oral care composition of claim 1, it is characterized in that: described heat-resistant superoxide dismutase is the Fe-SOD lyophilized powder extracted from a kind of extreme Thermophilic Bacteria (Aquifex pyrophilus).
3. oral care composition according to claim 1 and 2, its molecular weight of heat-resistant superoxide dismutase wherein is between 38700 to 40000, and concentration is 100-250U/ml.
4. according to the oral care composition of claim 1, it is characterized in that: described epithelical cell growth factor is at least one in lyophilized powder, stock solution form, and concentration is 0.2-20mg/ml.
5. according to the oral care composition of claim 1, it is characterized in that: the lipophilic group that the hydrophilic radical that described liposome is comprised of a phosphate and quaternary ammonium salt base and two longer alkyl form forms.
6. according to the oral care composition of claim 5, it is characterized in that: wherein liposome at least comprises one or more compositions in lecithin, cholesterol, ceramide, sphingomyelins, between mean diameter 300-1300nm.
7. according to the oral care composition of claim 1, its isoelectric point, IP is between 5-7.
8. the oral care composition of any one in aforementioned claim, the application in toothpaste, collutory gel, powder, confectionery and lozenge oral care product.
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104606063A (en) * | 2015-03-04 | 2015-05-13 | 王海龙 | Cosmetic active ingredient-containing lipidosome as well as preparation method and application thereof |
| CN105797201A (en) * | 2016-03-11 | 2016-07-27 | 康迪菲（苏州）生物科技有限公司 | Composite hydrocolloid and preparation method thereof and hydrocolloid dressing and preparation method of hydrocolloid dressing |
| CN106267159A (en) * | 2016-09-09 | 2017-01-04 | 拉芳家化股份有限公司 | A kind of oral care composition |
| CN107028839A (en) * | 2017-05-31 | 2017-08-11 | 易金阳 | Oral care composition and its application, dentifrice composition |
| CN117618282A (en) * | 2023-12-11 | 2024-03-01 | 广州中汉口腔用品有限公司 | Toothpaste formula containing active SOD enzyme and preparation method thereof |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1102978A (en) * | 1993-11-23 | 1995-05-31 | 姚焕新 | Multi-function toothpaste |
| US20070072793A1 (en) * | 2002-07-25 | 2007-03-29 | Yih-Lin Chung | Histone hyperacetylating agents for promoting wound healing and preventing scar formation |
| WO2008007728A1 (en) * | 2006-07-13 | 2008-01-17 | Kaneka Corporation | Oral preparation in the form enclosed in lipid membrane structure, and fatigue-ameliorating agent comprising coenzyme a as active ingredient |
| CN102210853A (en) * | 2010-04-01 | 2011-10-12 | 孙民富 | Method for preparing liposome containing EGFs (Epidermal Growth Factors) and transdermal short peptide fusion proteins, for controlling diabetic foot gangrene |
| CN102302416A (en) * | 2011-08-26 | 2012-01-04 | 广州佳禾化妆品制造有限公司 | Coenzyme Q-10/EGF liposome, preparation method and application |
| CN102949345A (en) * | 2012-06-15 | 2013-03-06 | 深圳职业技术学院 | Recombinant human epidermal growth factor cationic liposome and preparation method thereof |
-
2013
- 2013-09-11 CN CN201310413876.4A patent/CN103463627B/en active Active
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1102978A (en) * | 1993-11-23 | 1995-05-31 | 姚焕新 | Multi-function toothpaste |
| US20070072793A1 (en) * | 2002-07-25 | 2007-03-29 | Yih-Lin Chung | Histone hyperacetylating agents for promoting wound healing and preventing scar formation |
| WO2008007728A1 (en) * | 2006-07-13 | 2008-01-17 | Kaneka Corporation | Oral preparation in the form enclosed in lipid membrane structure, and fatigue-ameliorating agent comprising coenzyme a as active ingredient |
| CN102210853A (en) * | 2010-04-01 | 2011-10-12 | 孙民富 | Method for preparing liposome containing EGFs (Epidermal Growth Factors) and transdermal short peptide fusion proteins, for controlling diabetic foot gangrene |
| CN102302416A (en) * | 2011-08-26 | 2012-01-04 | 广州佳禾化妆品制造有限公司 | Coenzyme Q-10/EGF liposome, preparation method and application |
| CN102949345A (en) * | 2012-06-15 | 2013-03-06 | 深圳职业技术学院 | Recombinant human epidermal growth factor cationic liposome and preparation method thereof |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104606063A (en) * | 2015-03-04 | 2015-05-13 | 王海龙 | Cosmetic active ingredient-containing lipidosome as well as preparation method and application thereof |
| CN104606063B (en) * | 2015-03-04 | 2021-07-16 | 王海龙 | Liposome containing cosmetic active ingredients and preparation method and application thereof |
| CN105797201A (en) * | 2016-03-11 | 2016-07-27 | 康迪菲（苏州）生物科技有限公司 | Composite hydrocolloid and preparation method thereof and hydrocolloid dressing and preparation method of hydrocolloid dressing |
| CN106267159A (en) * | 2016-09-09 | 2017-01-04 | 拉芳家化股份有限公司 | A kind of oral care composition |
| CN107028839A (en) * | 2017-05-31 | 2017-08-11 | 易金阳 | Oral care composition and its application, dentifrice composition |
| CN117618282A (en) * | 2023-12-11 | 2024-03-01 | 广州中汉口腔用品有限公司 | Toothpaste formula containing active SOD enzyme and preparation method thereof |
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