KR101448222B1 - Cosmetic composition containing fruit vinegar - Google Patents

Abstract

The present invention relates to a cosmetic composition containing red vinegar, which is a fruit vinegar, and which comprises a cosmetic composition containing at least one fruit vinegar selected from the group consisting of pomegranates, omiza glaze, bokbunja glaze, red ginseng glaze, grape glaze, Is excellent in the effect of inhibiting the elastase, the effect of promoting the epidermal cell, the effect of promoting the production of hyaluronic acid, the effect of promoting the skin turnover, the effect of improving the skin keratin, improving the skin softness, improving the skin luster and improving skin elasticity.

Pomegranate, Omija, Bokbunja, Red ginseng, Grape, Odyssey, Paekryikcho, Cosmetics.

Description

COSMETIC COMPOSITION CONTAINING FRUIT VINEGAR [0002]

The present invention includes a fruit vinegar (red candle) derived from at least one of pomegranate, omija, bokbunja, red ginseng, grape, olive and pomegranate to provide an inhibitory effect of elastase, an effect of promoting epidermal cell, a stimulating effect of producing hyaluronic acid, An effect of improving skin skin smoothness, an effect of improving skin luster, and an effect of improving skin elasticity.

Vinegar is a seasoning containing acetic acid as its main ingredient and having a slightly sweet taste. It is a food which used seasoning to taste sour as original wine fermented. Vinegar not only stimulates appetite, but also contains several dozens of organic acids such as acetic acid, citric acid and various amino acids, which stimulates metabolism, helps eliminate body waste, and is effective in preventing constipation. As mentioned above, the vinegar's efficacy strengthens appetite, taste, metabolism, growth promoter and natural healing power, and is also known as a powerful antiseptic and powerful disinfectant, which means that vinegar purifies our body.

Vinegar can be divided into chemical synthetic vinegar and vinegar vinegar according to the raw materials, and vinegar vinegar can be further divided into vinegar, vinegar and fruit vinegar. Red corn corresponds to fruit vinegar whose main ingredient is red fruit. While the traditional vinegar is sour, it is burdensome to drink, while the red fruit vinegar is called 'drinking red ginseng' and it is known that anyone can enjoy it because it is low in acidity and soft. Hongcheon is characterized by fermentation aging as it is in nature like wine ripening.

The main component of vinegar is acetic acid, which is an organic acid that has a sterilizing and detoxifying action and contains carbon. In addition, vinegar contains more than 60 kinds of organic acids such as various amino acids, malic acid, succinic acid, and tartaric acid. Such an organic acid-rich food is very beneficial to human body as an enzyme. It is also known that organic acids are located in the moisture of tissues to make active oxygen trapped and safe. It also promotes the absorption of calcium and activates enzymes that destroy and find active oxygen. Organic acid and calcium are contained in the vinegar food, calcium acetate. Drinking vinegar rich in organic acids every day can prevent cataracts, cancer, and liver diseases.

Citric acid in organic acids is one of the ingredients needed to increase the oxygen utilization rate in our body. It activates metabolism in the body and helps to release energy and prevents waste from remaining in the body. When the nutrients are decomposed in our body, when the chemical reaction called citric acid circuit is normally performed, the nutrients are decomposed into water and carbon dioxide which are harmless to the human body and released.

However, when this process is not carried out smoothly, lactic acid, which is a fatigue substance, accumulates in the body. When lactic acid accumulates in the body, symptoms such as headache, shoulder stiffness and back pain are seen and it becomes illness. At this time, citric acid is the substance that stimulates the body metabolism by helping to release the fatigue substance. The effect of vinegar on the recovery and prevention of fatigue can be attributed to citric acid. Vinegar promotes metabolism rapidly and simultaneously releases waste products and various acidic substances from the body. As a result, the synthesis rate of the body fat is slowed or prevented, and the decomposition of fat is promoted, thereby preventing fat accumulation.

In addition, vinegar is known to function to restore the weakened kidney tissue.

Vinegar is a powerful antiseptic as well as a powerful disinfectant. Normally used chemical preservatives and disinfectants cause severe side effects in the body, but because vinegar protects the body and sterilizes and causes side effects, eating vinegar will clean our flesh and blood.

In addition, vinegar is known to inhibit the destruction of vitamin C, Dr Creves of the United Kingdom and Dr. Lippmann of the United States, vinegar has been found to prevent the occurrence of fatigue and lactic acid. In 1964, Dr. Brutzho of the United States and Dr. Linen of Germany reported that drinking vinegar produces a corticosteroid that relieves stress.

Thus, vinegar is reported or known to be mostly edible and folk remedies, and there is little research on the effect of vinegar on skin's efficacy when applied to skin. In addition, vinegar is divided into chemical vinegar, alcoholic vinegar, fruit vinegar, cereal vinegar, etc. in a large frame, and the number of starting vinegars that can make as many starting raw materials as each vinegar is as many as the starting raw materials Despite their diversity, the research on the differential effect of each vinegar on the skin is very limited. Therefore, there is a desperate need for studies on the specific effect of each of the vinegar which can be developed in various ways.

As a result of intensive studies based on the necessity of such studies, the present inventors have found that each of red algae produced from pomegranate, omija, bokbunja, red ginseng, grape, It has excellent skin safety but also excellent effect of inhibiting elastase, effect of epidermal cell proliferation, promotion of hyaluronic acid production ability, promotion of skin turnover, effect of improving skin dermatitis, improvement of skin softness, improvement of skin glossiness and improvement of skin elasticity And discovered the surprising fact, thereby completing the present invention.

It is an object of the present invention to provide a cosmetic composition which is superior in skin safety to vinegar, yet exhibits an effect of inhibiting elastase, a skin cell proliferation effect, a stimulating effect of producing hyaluronic acid, a skin turnover promoting effect, a skin keratinization effect, And an effect of improving skin elasticity.

In order to achieve the above object, the present invention provides a cosmetic composition comprising at least one fruit vinegar selected from the group consisting of pomegranate, omiza, bombonic, red ginseng, grape,

Here, the content of the fruit vinegar is preferably 0.01 to 30.0% by weight of the total weight of the cosmetic.

In addition, the fruit vinegar may be prepared by fermenting pomegranate, omija, bokbunja, red ginseng, grape, oder, and paulsikcho with yeast for 3 months or more.

Hereinafter, the present invention will be described in detail.

The natural products used in the production of red ginseng constituting the present invention are pomegranate, omija, bokbunja, red ginseng, grape, oder, paulikwi.

[Production method of red candle]

First, pomegranate, omija, bokbunja, red ginseng, grape, oudy, pauliksikcho clean pure water to remove fine particles and other foreign matter. If each natural product is scratched, it is cut off and the shell and seed of each natural product are used. Next, these natural products are taken out and finely crushed, and the crushed natural products are put into each bin. At this time, only about 70% of the container is filled and the upper 30% is left empty. Then add yeast and mix well to penetrate the whole raw material. Next, the lid of the brewing vessel for fermentation is wrapped with a double wrapped cloth so as to allow air to pass through. It is fermented for 3 months or more in a place where the direct sunlight is not visible, the temperature is relatively constant, and air communication is good. In this way, it is possible to produce pomegranate, omiza, bokbunja, red ginseng, grape, oedicos, and paprikikoso, respectively, by squeezing or screening the mullet.

The cosmetic composition of the present invention preferably contains the red acacia so produced in an amount of 0.01 to 30.0% by weight. If the amount of red ginseng is more than 30.0% by weight of the cosmetic composition, there is a problem in workability during the production of cosmetics, and if it is less than 0.01% by weight, the effect is not expected.

The cosmetic composition comprising the pomegranate, the ozimizo, the brambunctal, the red ginseng, the grape, the oddicum, the pomegranate and the at least one kind of the red and thyme mixture according to the present invention may be in any form such as a liquid, a cream, a paste or a solid Is easy to apply. That is, the composition of the present invention can be applied variously and easily to cosmetic formulations such as creams and lotions, and soap formulations.

According to the present invention, a cream containing a mixture of red ginseng extract of Pomegranate mushroom, Omiza gum, Bokbunja ginseng, Red ginseng, Grape gum, Odicho, and Paekryikcho ginseng is excellent in improvement of skin texture, skin softness, skin gloss and skin elasticity .

Hereinafter, the present invention will be described in detail.

The natural products used in the production of red ginseng constituting the present invention are pomegranate, omija, bokbunja, red ginseng, grape, oder, and prairiecho.

Manufacturing example  1: Red flour manufacture

The process for preparing the red ginseng used in the present invention is as follows:

First, pomegranate, omija, bokbunja, red ginseng, grape, odi, and paulsikcho were cleaned with pure purified water to remove fine particles and other foreign matter. If each natural product is scratched, it is cut off and the shell and seed of each natural product are used as it is. Then, these natural products were taken out and finely crushed, and 1000 g of the crushed natural products were put into respective bins. At this time, only about 70% of the containers were filled and about 30% of the containers were left empty. Then, 20 g of yeast was added and mixed thoroughly into the whole raw material. Next, the lid of the brewing vessel for fermentation was covered with a double layer of air to be passed through, and the mixture was run with a cord, followed by fermentation for 3 months in a place where the direct sunlight was not visible and the temperature was relatively constant and air communication was good. In this manner, ripe ones were squeezed out or sifted to prepare 100 ml of pomegranate juice, 90 ml of omiza juice, 110 ml of bokbunja juice, 70 ml of red ginseng, 120 ml of grape juice, 80 ml of acetic acid,

Red  In Vitro Efficacy Assessment

1) Preparation of samples for efficacy evaluation (Reagent and Comparative Examples)

Comparative Example 1: Purified water

Comparative Example 2: Synthetic vinegar

Comparative Example 3: Mechos (rice)

Comparative Example 4:

Example 1: Pomegranate syrup prepared in the above preparation example

Example 2: Preparation of Omija candle

Example 3: The bacteriocidal candle prepared in the above preparation example

Example 4: Red ginseng root prepared in the above preparation example

Example 5: Preparation of grape seeds

Example 6: Preparation of Odyssey

Example 7: Preparation of paclitaxel

Example 8: Pomegranate: Omija chitosan prepared in the above preparation example = 1: 1

Example 9: Omiza chorus: Rubus chinensis prepared in the above preparation example = 1: 1

Example 10: Pomegranate: Omija-zo: bokbunja-chi = 1: 1: 1

Example 11: Red ginseng prepared in the above preparation example: grape juice = 1: 1

Example 12: Red ginseng prepared in the above preparation example: grape juice: Odicho = 1: 1: 1

Example 13: Red ginseng prepared in the above preparation example: grape juice: Odicho: Paekryikcho second = 1: 1: 1: 1

Example 14: Pomegranate prepared in the above preparation example: Omiza chitosan: bokbunja chitosan: red ginseng chop: grape chod: Odicho = 1: 1: 1: 1: 1: 1

Example 15: Pomegranate juice prepared in the above preparation example: Omiza juice: bokbunja juice: red ginseng juice: grape juice: orange juice: pearly juice juice = 1: 1: 1: 1: 1: 1: 1

2) In Vitro efficacy evaluation test

(1) Elastase enzyme inhibition test (In Vitro)

A total of 19 samples of Comparative Examples 1 to 4 and Examples 1 to 15 were tested for inhibition of elastase enzyme activity, which is an important factor for improving skin elasticity, and compared with the negative control group.

25 μl of synthetic substrate Methoxysuccinyl-Ala-Ala-Pro-p-Nitroanilide (8.0 mM, 25 μl) was added to 5 μg / ml of human neutrophil-derived elastase-containing 0.1 M Hepes buffer (pH 7.4, containing 0.5 M NaCl) And the above evaluation sample at 37 캜 for 30 minutes. Thereafter, the amount of 4-nitroaniline produced as a decomposition product was measured by a microplate reader at 405 nm. The enzyme inhibition rate was calculated according to the following equation (1).

[Equation 1]

Inhibition rate (%) = (A-B) / A x 100

A: absorbance of sample not added visibly, B: absorbance at the time of adding sample

Table 1 below shows the evaluation results of the inhibition rate of elastase (%), and the inhibition rate (%) at the concentration of 0.1% of the sample.

sample Inhibition rate (%) Comparative Example 1 0 Comparative Example 2 5.2 Comparative Example 3 7.3 Comparative Example 4 7.1 Example 1 60.2 Example 2 60.4 Example 3 70.1 Example 4 64.1 Example 5 62.1 Example 6 68.1 Example 7 67.4 Example 8 75.2 Example 9 77.5 Example 10 78.1 Example 11 74.1 Example 12 80.1 Example 13 84.2 Example 14 80.1 Example 15 84.2

As shown in Table 1, in comparison with the purified water, the synthetic vinegar, the grain vinegar (rice germination) and the vinegar of Comparative Examples 1 to 4, the pomegranate, omija, bokbunja, red ginseng, Chondroitin, chondroitin sulfate, chondroitin sulfate, chondroitin sulfate, chondroitin sulfate, chondroitin sulfate, chondroitin sulfate and chondroitin sulfate showed remarkable effects on the inhibitory activity against elastase.

(2) Human-derived epidermal cell proliferation test (In Vitro)

The epidermal cells collected from the human body were treated with 0.01% of the total of 19 samples of Comparative Examples 1 to 4 and Examples 1 to 15 to measure the proliferation effect of epidermal cells.

Human epidermal cells cultured in DMEM (Dulbecco's Modified Eagle's Media) medium containing 2.5% fetal bovine serum were dispensed in a 96-well plate incubator at 5 × 10 ³ cells / well. The above samples were cultured by treating 100 μl twice a day for 3 days and then 0.2% MTT (3- [4,5-dimethylthiodiazol-2-yl] -2,5-phenyltertazolium bromide) And the mixture was incubated at 37 ° C for 4 hours. The resultant formazan (product of mitochondrial dehydrogenase and MTT reaction) was dissolved in dimethyl sulfoxide (DMSO). The absorbance of dissolved Formazan was measured at 570 nm using a microplate reader. Here, the untreated group was used as a control group.

The calculated absorbance is used to calculate the epidermal cell proliferation effect of the above samples. The absorbance is proportional to the amount of the cells because the absorbance increases when the cell proliferation increases. The epidermal cell proliferation effect was calculated by the following equation (2) by comparing the absorbance of the control group with the absorbance of each sample, and the results are shown in Table 2.

&Quot; (2) "

Proliferation rate (%) = (absorbance of test group-absorbance of control group) / absorbance of control group 100

sample Growth rate (%) Comparative Example 1 0 Comparative Example 2 10.2 Comparative Example 3 10.5 Comparative Example 4 9.5 Example 1 67.2 Example 2 60.1 Example 3 68.2 Example 4 54.1 Example 5 65.1 Example 6 55.1 Example 7 58.4 Example 8 58.2 Example 9 67.5 Example 10 60.1 Example 11 74.1 Example 12 78.1 Example 13 80.1 Example 14 80.21 Example 15 80.7

As shown in the above Table 2, in comparison with the purified water, the synthetic vinegar, the grain vinegar and the vinegar of Comparative Examples 1 to 4, the pomegranate, omija, bokbunja, red ginseng, , Odicho, and Paekryikcho candles, or one or more kinds of red alchohol mixtures showed excellent epidermal cell proliferation effect.

(3) Test for promoting production of hyaluronic acid (In Vitro)

Hyaluronic acid performs many functions such as maintenance of moisture in the cell gap, maintenance of cells by formation of a jell-shaped matrix in the tissue, maintenance of the lubrication and flexibility of the tissue, resistance to external force, and prevention of bacterial infection. Skin fibrotic proteins are responsible for maintaining tissue morphology or imparting elasticity to the skin. Therefore, when the production of fibrous protein is promoted, the skin becomes elastic and becomes firm skin. One of the typical aging phenomena due to skin cell protein deficiency is reduced skin elasticity. When a skin-binding protein is deficient, the binding force between the epidermal cells and the binding force between the epidermis and the dermis is weakened, and when the skin is thinned, the elasticity is reduced. To improve this elasticity reduction, there is a method of promoting the binding protein metabolism. One of the experimental models is a test for promoting the production of hyaluronic acid, which is one of the skin binding proteins.

In the present test, the action of 19 samples in Comparative Examples 1 to 4 and Examples 1 to 15 on the hyaluronic acid production ability of epidermal cells was evaluated using human epidermal cells.

The epidermal cells (5 x 10 ⁴ cells / well) suspended in KGM (epidermal cell culture medium) were inoculated into a 24-well culture plate and cultured in an incubator (37 ° C, 5% CO ₂ ) until becoming a confluent Respectively. Subsequently, the medium was changed to a medium for epidermal cells containing 10 μg / ml of the sample and a medium for medium for sample addition, and cultured for 48 hours under the same conditions. After 48 hours, the cultured upper layer was collected and the amount of hyaluronic acid in the culture supernatant was measured. The amount of hyaluronic acid was measured using a hyaluronic acid plate (measurement kit). Table 3 shows the effect of hyaluronic acid production, which is the result of the test at 10 占 퐂 / ml of each sample. The indicators of evaluation are based on the following criteria.

-: no formation (negative control)

+: Formation is confirmed, but weak

++: confirmation of formation

+++: Identification of distinct formation

sample Generation effect Comparative Example 1 - Comparative Example 2 - Comparative Example 3 + Comparative Example 4 - Example 1 +++ Example 2 +++ Example 3 +++ Example 4 +++ Example 5 +++ Example 6 +++ Example 7 +++ Example 8 +++ Example 9 +++ Example 10 +++ Example 11 +++ Example 12 +++ Example 13 +++ Example 14 +++ Example 15 +++

As shown in the above Table 3, in comparison with the purified water, the synthetic vinegar, the grain vinegar and the vinegar of Comparative Examples 1 to 4, the pomegranate, the omeza, the bramble root, the red ginseng, , Odicho, and Paekryikcho ginseng, or one or more kinds of red flush mixture showed excellent hyaluronic acid generation effect.

Evaluation of human body efficacy of reeds :

Test to confirm skin turnover promotion effect in human skin

A total of 19 samples of Comparative Examples 1 to 4 and Examples 1 to 15 and a skin turnover promoting effect of a sample untreated control group were examined.

Before the application of the sample, the inside color of the upper arm of the subject was measured with a chromaticity meter, and the cream containing dihydroxyacetone (DHA) was used to artificially tannify 50 healthy men and women (mean age 31 years) The color difference between before and after application was compared. Thereafter, the samples obtained according to the above Examples and Comparative Examples were dissolved by 1.0 weight% in a mixed solvent (water: ethanol: butylene glycol = 80: 15: 5) twice a day, And the time taken to return to the original skin color (change in chromaticity due to protein discoloration = skin turnover time) was measured. The skin turnover promotion rate was calculated by the following equation (3), and the results are shown in Table 4 below. Table 4 shows the skin turnover promotion rate (%) of the samples of the Examples and Comparative Examples.

&Quot; (3) "

Turnover promoting rate (%) = (X-Y) / X x 100

X is the sample-free turnover time, and Y is the turnover time at the sample application.

Here, the turnover time is the time taken for the existing stratum corneum to be replaced with a new stratum corneum, i.e., the time it takes for the skin to return to its original shape after being tanned by DHA.

sample Acceleration rate (%) Comparative Example 1 0 Comparative Example 2 3.5 Comparative Example 3 5.5 Comparative Example 4 5.8 Example 1 29.3 Example 2 28.2 Example 3 30.2 Example 4 31.1 Example 5 33.2 Example 6 39.2 Example 7 31.2 Example 8 28.9 Example 9 29.9 Example 10 31.2 Example 11 31.8 Example 12 28.7 Example 13 29.8 Example 14 29.9 Example 15 34.2

As shown in the above Table 4, in comparison with the purified water, the synthetic vinegar, the grain vinegar and the vinegar of Comparative Examples 1 to 4, the pomegranate, the omeza, the bramble root, the red ginseng, , Odicho, and Paekyikcho candles or one or more kinds of red leaf blend liquids were excellent in the turnover promoting effect of shortening the skin turnover cycle, and thus the cell regeneration ability was excellent.

From the results of the above experiment, it can be seen that the single or one or more kinds of red ginseng mixture of pomegranate, omiza, ginseng, red ginseng, grape ginseng, It was evaluated that the effect of inhibiting the star action, the effect of proliferation of human epidermal cells, the effect of promoting production of hyaluronic acid, and the effect of promoting skin turnover in human skin were excellent.

Hereinafter, the cosmetic composition of the present invention will be described by taking a cleanser and cream as an example. Herein, the cleanser and the cream can be easily prepared according to a conventional method.

Manufacturing example  2: Manufacture of cleanser

According to the prescription of Table 5 below, 99.0% by weight of the cleanser base (including water) and 1.0% by weight of the red ginseng or the red ginseng of the example were homogenously mixed in a homomixer through stirring, homogenization, heating, cooling and defoaming , The cleanser of Examples 16 to 19 and Comparative Examples 5 and 6 was prepared according to a conventional method. In Table 5 below, the unit is wt%.

Raw material name Example 16 Example 17 Example 18 Example 19 Comparative Example 5 Comparative Example 6 1. Lauric Acid 10.0 10.0 10.0 10.0 10.0 10.0 2. Myristic acid 20.0 20.0 20.0 20.0 20.0 20.0 3. Potassium hydroxide Suitable amount Suitable amount Suitable amount Suitable amount Suitable amount Suitable amount 4. Tetrasodium EDTA 0.1 0.1 0.1 0.1 0.1 0.1 5. Glycerin 10.0 10.0 10.0 10.0 10.0 10.0 6. Red candle of Example 1 1.0 - - - - - 7. Red candle of Example 9 - 1.0 - - - - 8. Reagan of Example 13 - - 1.0 - - - 9. Purple candle of Example 15 - - - 1.0 - - 10. Comparative Example 3 - - - - - 1.0 11. Spices Suitable amount Suitable amount Suitable amount Suitable amount Suitable amount Suitable amount 12. Purified water Suitable amount Suitable amount Suitable amount Suitable amount Suitable amount Suitable amount

effect Test Example  One: Red candle  Evaluation of improving skin condition of cleanser containing

(1) Selection of the evaluator

In order to evaluate the clinical efficacy of the cleanser containing red ginseng, 120 applicants were selected through questionnaires and expert interviews for clinical applicants who agreed to the clinical study. Eighty-two out of 120 patients were required to use 20 of the cleansers of Examples 16 to 20 and the remaining 40 patients to use 20 of the cleansers of Comparative Examples 5 and 6.

(2) Evaluation method

The subject first wet the face with light running water, then applied the cleansing agent to the face and gently massage the entire face gently. Massage was applied to the face over the entire face for about 30 seconds, and then rinsed cleanly in flowing water.

After the evaluation of the cleansing, resting was carried out in a constant temperature and humidity room (25 ° C, 50-65%) for 10 minutes and evaluation of the improvement of the human and mental condition before and after the evaluation was made through the prepared questionnaire.

Table 6 below shows the evaluator's evaluation sheet on the degree of skin condition improvement.

Check symptoms Very bad Slightly worse No change Slightly improved Very good Skin keratinization -2 -One 0 +1 +2 Skin softness -2 -One 0 +1 +2 With skin shine -2 -One 0 +1 +2 With skin elasticity -2 -One 0 +1 +2

The following Tables 7 to 12 show evaluation results of the evaluators in the groups using the cleansers of Examples 16 to 19, Comparative Examples 5 and 6, respectively. In Tables 7 to 12, the units are numbers (%).

Example 16 Using a cleanser group Check symptoms Very bad Slightly worse No change Slightly improved Very good Skin keratinization 0 0 4 (20%) 10 (50%) 6 (30%) Skin softness 0 0 4 (20%) 9 (45%) 7 (35%) With skin shine 0 0 4 (20%) 11 (55%) 5 (25%) With skin elasticity 0 0 3 (15%) 10 (50%) 7 (35%)

Example 17 Using the cleanser group Check symptoms Very bad Slightly worse No change Slightly improved Very good Skin keratinization 0 0 3 (15%) 11 (55%) 6 (30%) Skin softness 0 0 3 (15%) 10 (55%) 7 (35%) With skin shine 0 0 3 (15%) 12 (60%) 5 (25%) With skin elasticity 0 0 3 (15%) 10 (50%) 7 (35%)

Example 18 Using a cleanser group Check symptoms Very bad Slightly worse No change Slightly improved Very good Skin keratinization 0 0 3 (15%) 10 (50%) 7 (35%) Skin softness 0 0 4 (20%) 9 (45%) 7 (35%) With skin shine 0 0 4 (20%) 11 (55%) 5 (25%) With skin elasticity 0 0 3 (15%) 10 (50%) 7 (35%)

Example 19 Use of cleanser group Check symptoms Very bad Slightly worse No change Slightly improved Very good Skin keratinization 0 0 2 (10%) 11 (55%) 7 (35%) Skin softness 0 0 3 (15%) 10 (50%) 7 (35%) With skin shine 0 0 4 (20%) 12 (60%) 6 (30%) With skin elasticity 0 0 3 (15%) 10 (50%) 7 (35%)

Comparative Example 5 Using the cleanser group Check symptoms Very bad Slightly worse No change Slightly improved Very good Skin keratinization 0 1 (5%) 16 (80%) 3 (15%) 0 Skin softness 0 2 (10%) 16 (80%) 2 (10%) 0 With skin shine 0 3 (15%) 14 (70%) 3 (15%) 0 With skin elasticity 0 4 (20%) 14 (70%) 2 (10%) 0

Comparative Example 6 Using the cleanser group Check symptoms Very bad Slightly worse No change Slightly improved Very good Skin keratinization 0 2 (10%) 16 (80%) 2 (10%) 0 Skin softness 0 2 (10%) 16 (80%) 2 (10%) 0 With skin shine 0 3 (15%) 14 (70%) 3 (15%) 0 With skin elasticity 0 3 (15%) 14 (70%) 3 (15%) 0

As can be seen from the above experimental results, the testee using a single cleanser containing a mixture of pomegranates, omiza, brambles, red ginseng, grape, Improvement in skin softness, improvement in skin luster and improvement in skin elasticity were evaluated as excellent.

Manufacturing example  3: Cream  Produce

According to the prescription of Table 13 below, 0.1% by weight of Reishi of Example 15 was well emulsified in a mixer to 99.9% by weight (including water) of a combination of an oily component, an aqueous component and a surfactant as a cream base and the mixture was degassed, To prepare a cream composition. If necessary, preservatives, chelating agents, fragrances and pigments can be added, and prescriptions are made in water type (O / W) so that a small amount of oily components are present. In Table 13 below, the unit is wt%.

Raw material name Example 20 Example 21 Comparative Example 7  1. Stearic acid  8.00  8.00  8.00  2. Stearyl alcohol  4.00  4.00  4.00  3. Butyl stearate  6.00  6.00  6.00  4. Propylene glycol  5.00  5.00  5.00  5. Glycerin monostearate  2.00  2.00  2.00  6. Potassium hydroxide Suitable amount Suitable amount Suitable amount  7. Reeduces of Example 15 0.1 1.0 -  8. Spices Suitable amount Suitable amount Suitable amount  9. Preservatives Suitable amount Suitable amount Suitable amount 10. Purified water Suitable amount Suitable amount Suitable amount

effect Test Example  3: Red candle  Evaluation of skin condition improvement effect of containing cream

(1) Selection of the evaluator

To evaluate the clinical efficacy of creams containing red ginseng, 60 respondents were selected through questionnaires and expert interviews for clinical participants who agreed to the clinical trial. 40 of the 60 patients were required to use the cream preparations of Examples 20 and 21 for 20 patients each, and the remaining 20 patients were to use 20 toilet preparations of Comparative Example 7.

(2) Evaluation method

The respondent first washed his face with clean water and gently massage the entire face gently to the full face, taking away enough cream in his hands. The massage was performed on the face over the entire face for about one minute.

After the evaluation of the cream massage, rest was taken in a constant temperature and humidity room (25 ° C, 50-65%) for 10 minutes, and the improvement of the human and mental condition before and after the evaluation was evaluated through the prepared questionnaire.

The following Table 14 is the evaluator's evaluation sheet on the degree of skin condition improvement.

Check symptoms Very bad Slightly worse No change Slightly improved Very good Skin exfoliation -2 -One 0 +1 +2 Skin softness -2 -One 0 +1 +2 With skin shine -2 -One 0 +1 +2 With skin elasticity -2 -One 0 +1 +2

The following Tables 15 to 17 show the evaluation results of the evaluators in the groups using the creams of Examples 20 and 21 and Comparative Example 7, respectively. In Tables 15 to 17, the units are numbers (%).

Example 20 Cream use group Check symptoms Very bad Slightly worse No change Slightly improved Very good Skin exfoliation 0 0 2 (10%) 12 (60%) 6 (30%) Skin softness 0 0 2 (10%) 11 (55%) 7 (35%) With skin shine 0 0 2 (10%) 13 (65%) 5 (25%) With skin elasticity 0 0 2 (10%) 11 (55%) 7 (35%)

Example 21 Cream use group Check symptoms Very bad Slightly worse No change Slightly improved Very good Skin exfoliation 0 0 1 (5%) 11 (55%) 8 (40%) Skin softness 0 0 1 (5%) 12 (60%) 7 (35%) With skin shine 0 0 2 (10%) 12 (60%) 6 (30%) With skin elasticity 0 0 1 (5%) 10 (50%) 9 (45%)

Comparative Example 7 Cream-use group Check symptoms Very bad Slightly worse No change Slightly improved Very good Skin exfoliation 0 3 (15%) 16 (80%) 1 (5%) 0 Skin softness 0 2 (10%) 17 (85%) 1 (5%) 0 With skin shine 0 2 (10%) 15 (75%) 3 (15%) 0 With skin elasticity 0 3 (15%) 15 (75%) 2 (10%) 0

As shown in the above test results, the testee using the cream containing the mixture of the pomegranate juice, omiza juice, bokbunja juice, red ginseng juice, grape juice, Improvement of skin gloss and improvement of skin elasticity were evaluated as excellent.

Claims (4)

A cosmetic composition having an elastase inhibitory effect, characterized in that it contains a red candle mixture comprising at least two kinds of red candles selected from pomegranate candle, omija candle, bokbunja candle, red ginseng candle, grape candle, The cosmetic composition according to claim 1, wherein the content of the red and green basal mixture is 0.01 to 30% by weight of the total weight of the cosmetic composition. The cosmetic composition according to claim 1, wherein the red ginseng is produced by fermenting pomegranate, omija, bokbunja, red ginseng, grape, oder and paulsikcho respectively with yeast for 3 months or longer. delete