KR101436753B1 - Anti obesity composition comprising licorice extracts and preparation method thereof - Google Patents

Abstract

The present invention provides a composition for enhancing endurance or athletic performance comprising the licorice extract as an active ingredient, and more particularly, to a composition for maximizing the content of effective ingredients judged to be related to such a function to provide more excellent effects on endurance or athletic performance And a method for producing the same.

Description

TECHNICAL FIELD [0001] The present invention relates to a composition for inhibiting obesity, which contains an extract of licorice as an active ingredient, and a method for producing the composition.

The present invention relates to a composition containing an extract of licorice root as an active ingredient and more particularly to a composition for endurance or athletic performance enhancement containing a licorice extract and a method of producing the same, To provide a more excellent effect on endurance or athletic performance, and a method of manufacturing the same.

Research into the use of adjuvants, functional foods, food compositions, etc. to improve physical athletic performance has been ongoing for a long time and actually taking a compound such as steroids, caffeine, etc. may increase exercise capacity. These drugs, however, can be accompanied by fatal side effects, so their use is extremely limited.

Glycyrrhizin has been used as a food seasoning or medicament for a long time. In particular, Glycyrrhizin, which is a main ingredient, has a sensitivity of 150 times that of sucrose and is widely used as a sweetener. On the other hand, it is known that licorice extract has a pharmacological action such as anti-ulcer action (Takagi, K., Ishii, Y.: Arzein Forsch, 17, 1544 (1967)) or hepatic injury protection.

There have been studies on these various effects of licorice, but there is no known effect of enhancing physical exercise ability. In the present invention, it has been found that the extract of licorice can satisfy this demand while seeking a natural material which has no fatal side effect as a herbal ingredient and has a marked effect of improving endurance or athletic performance improvement.

Disclosure of Invention Technical Problem [8] Accordingly, the present invention has been made to solve the above-mentioned problems of the prior art, and its object is to provide a composition for enhancing endurance or athletic performance containing the licorice extract and a manufacturing method thereof.

Another object of the present invention is to provide a composition for maximizing the content of active ingredients, which is considered to be related to endurance or athletic performance improvement, to provide more excellent effects on endurance or athletic performance, and a method for producing the same.

The technical problem of the present invention as described above is achieved by the following means.

(1) A composition for enhancing endurance or athletic performance containing an licorice extract as an active ingredient.

(2) The method according to claim 1,

Wherein the licorice extract is a hot-water extract.

(3) The method according to claim 1,

Wherein the licorice extract is a supercritical extract comprising an ethanol extract or ethanol as an extraction solvent.

(4) The method according to claim 1,

Wherein the licorice extract is a mixture of a hot water extract and an ethanol extract which is a precipitate obtained in the hot water extraction process.

(5) The method according to claim 1,

Wherein the licorice extract is a mixture of an ethanol extract and a hot water extract of the precipitate obtained in the ethanol extraction step.

(6) The method according to claim 1,

The composition for enhancing endurance or athletic performance as claimed in claim 1, wherein the composition further comprises an extract.

(7) extracting licorice with hot water to obtain a first extract mainly containing glycyrrhizin as an active ingredient;

Extracting the precipitate obtained in the hot water extraction step with ethanol or supercritical extraction to obtain a second extract containing glyburidine as an active ingredient; And

Mixing the first extract and the second extract;

Wherein the method comprises the steps of:

(8) extracting licorice with ethanol or supercritical extraction to obtain a first extract mainly containing glabridin as an active ingredient;

Extracting the precipitate obtained in the ethanol extraction step with hot water to obtain a second extract mainly containing glycyrrhizin as an active ingredient; And

Mixing the first extract and the second extract;

Wherein the method comprises the steps of:

As described in detail above, the composition containing the licorice extract according to the present invention provides an effect of improving endurance or athletic performance in addition to the conventional functions known in the art. In particular, it is possible to provide a composition that maximizes the content of effective ingredients that are considered to be related to such a function, thereby providing more excellent effects on endurance or athletic performance.

FIG. 1 is a chromatogram of HPLC analysis of extracted solvents for licorice.
FIG. 2 shows changes in the content of functional ingredients according to extraction conditions for licorice.
FIG. 3 shows the results of measuring the effect of the high-fat dietary extract of licorice extract on the weight gain of exercise mice.
FIG. 4 shows the results of measuring the weight gain of each experimental group using the extract of the present invention.
FIG. 5 is a graph showing the average daily dietary intake of the mice tested with the extract of the present invention.
FIG. 6 shows the results of exercise endurance test after 6 and 9 weeks of the experimental diet experiment using the extract of the present invention.
FIG. 7 is a graph comparing long term weights of mice according to the kind of licorice extracts according to the present invention.
FIG. 8 shows the results of histological analysis of liver, white fat and brown fat using the extract of the present invention.
FIG. 9 shows the result of measurement of blood lipid changes by using the extract of the present invention.
FIG. 10 shows changes in total fat mass, TC and TG in liver tissues experimented with the extract of the present invention.
Figure 11 shows changes in CPK and glycogen content in the liver after the experiment using the extract of the present invention.
Figure 12 shows the changes in lactic acid and glycogen content of muscle tested with the extract of the present invention
Figure 13 shows the changes in lactic acid and glycogen content of muscle tested with the extract of the present invention
FIG. 14 shows gene expression of troponin I and myHCIIb, a slow and aerobic type I muscle marker, using the extract according to the present invention.
FIG. 15 is a result of measurement of fat cell killing effect by the mixed application of licorice extract and lettuce extract according to the present invention.

Hereinafter, the contents of the present invention will be described in more detail.

The present invention includes a composition for enhancing endurance or athletic performance containing an licorice extract as an active ingredient.

The licorice extract in the composition of the present invention includes hot water extract, ethanol extract (including a mixture with water, hereinafter collectively referred to as an ethanol extract unless otherwise noted), supercritical extract or a mixture of these extracts.

In the case of the hot-water extract, it is preferable to add 1 to 10 times of water and extract at 1 to 2 atm and 80 to 120 캜 for 1 to 12 hours. The ethanol extract may be an extraction solvent composed of 50 to 100% ethanol, and the supercritical extract may be performed under the pressure of 250 to 350 bar using ethanol as an extraction solvent.

The licorice extract in the composition of the present invention contains glycyrrhizin and / or gabridin as the main active ingredient, and these factors are directly or indirectly attributed to enhancement of endurance or athletic ability by the mechanism not yet revealed. In the present invention, it was confirmed that the licorice extract containing these components contained mainly glycyrrhizin in the case of the hot-water extract, and the glabridin was contained as the main component in the ethanol extract and the supercritical extract. Or exercise capacity of the human body.

In this sense, the licorice extract in the present invention is preferably a hydrothermal extract, a mixed extract of an ethanol extract or a supercritical extract. In order to maximize the yield, the licorice is extracted with hot water to obtain a first extract, and the precipitate obtained in the first extraction step is extracted again with ethanol or the like by supercritical extraction method to obtain a solution containing a large amount of glycyrrhizin and glabridin An extract can be obtained. Likewise, extracting the licorice with ethanol or the supercritical extraction method to obtain the first extract, and then extracting the precipitate obtained in the first extraction step again by the hot water extraction method can maximize the yield of the active ingredient It is possible.

In the present invention, it is preferable that the precipitate obtained in the second extraction step in each case further contains an extract once again extracted with hexane, chloroform, or ethyl acetate. These extracts can be added in a range of 0.1 to 10% by weight relative to the weight of the whole composition. In the experimental group to which these extracts were further added, the effect of improving endurance or athletic performance was better than that of other composition groups .

The composition of the present invention may further include an extract of Aldergia to further enhance the activity of inhibiting obesity, in addition to the above-described effect of improving endurance or athletic performance. The extract can be an extract such as hot water extract or ethanol, and according to the experiment of the present inventor, it was confirmed that the activity of inhibiting obesity is excellent without inhibiting the exercise capacity. It is preferable that such an extract is added in an amount of 1 to 10% by weight relative to the content of the whole composition.

The composition according to the present invention can be used variously in medicines, foods and beverages. Examples of the foods to which the extract of the present invention can be added include various foods such as beverages, gums, tea, vitamin complexes and health supplements. Examples of such foods include pills, powders, granules, infusions, tablets, Can be used. At this time, the amount of the extract in the food or beverage may generally be from 0.05 to 30% by weight of the total food weight of the health food composition of the present invention, and 0.05 to 30 g based on 100 ml of the health beverage composition have.

Food additive additives that can be used herein include food additives customary in the art, such as flavoring agents, flavoring agents, coloring agents, fillers, stabilizers, and the like. The health beverage composition according to the present invention is not particularly limited as to the ingredient to be added in addition to the above extract as an essential ingredient in the indicated ratio, and may contain various flavors or natural carbohydrates as an additional ingredient such as ordinary beverages. Examples of the natural carbohydrate include monosaccharides such as glucose, fructose and the like; Disaccharides such as maltose, sucrose, etc .; Polysaccharides such as dextrin, cyclodextrin and the like; And sugar alcohols such as xylitol, sorbitol, and erythritol. It is also possible to use natural flavoring agents (tau martin, stevia extract (for example, rebaudioside A, glycyrrhizin and the like) and synthetic flavoring agents (saccharin, aspartame, etc.) as flavors other than the above . The ratio of the natural carbohydrate is generally about 0.5 to 30 g per 100 ml of the composition of the present invention.

In addition to the above, the composition for enhancing endurance or athletic performance of the present invention may contain flavorings such as various nutrients, vitamins, minerals (electrolytes), synthetic flavors and natural flavors, colorants and fillers (cheese, chocolate etc.), pectic acid and its salts , Alginic acid and its salts, organic acids, protective colloid thickeners, pH adjusting agents, stabilizers, preservatives, glycerin, alcohols, carbonating agents used in carbonated drinks and the like.

In addition, the composition of the present invention may contain natural fruit juice and pulp for the production of fruit juice drinks and vegetable drinks. These components may be used independently or in combination. The ratio of such additives is not particularly limited, but is generally selected in the range of 0 to about 10 parts by weight based on 100 parts by weight of the composition of the present invention.

The composition for enhancing endurance or athletic performance containing the licorice extract of the present invention as an active ingredient may be administered parenterally or orally in accordance with the purpose and may be administered in an amount of 0.001 to 5 g per 1 kg of body weight per day Can be administered separately. The dosage level for a particular patient may vary depending on the patient's body weight, age, sex, health condition, diet, time of administration, method of administration, rate of absorption, severity of the disease,

Hereinafter, the present invention will be described in more detail with reference to Examples. It is to be understood, however, that these examples are provided so that the scope of the present invention is not limited thereto.

Example 1 Preparation of crude extracts by extraction solvent

Extraction of 95% ethanol and supercritical extraction of licorice were carried out to measure the change of the objective functional component eluted according to the polarity of the extraction solvent. Table 1 shows the extraction conditions and yields according to the extraction solvent.

Extraction conditions and yield Extraction type menstruum Amount of solvent Extraction condition Yield (%) Hot water extraction water x 10 100 ° C, 4 hrs 16.3 EtOH Extraction 70% EtOH x 10 RT, overnight 10.9 Supercritical extraction 20% EtOH x 7 300 bar, 3.56

The changes in dissolution of functional components in supercritical extracts with two solvents (hot water, ethanol), which are contrasted with polarity differences, were confirmed by HPLC analysis using the established simultaneous analysis conditions (Fig. 1) (Fig. 2).

Glycyrrhizic acid content was significantly higher in the case of hot water extracts using polar solvents. In the case of the 95% ethanol extract using a nonpolar solvent, the elution of glycyrrhizic acid was very low and a little amount of glabridin was eluted. On the contrary, in case of supercritical extraction, it was confirmed that the content of glycyrrhizic acid was low and that large amount of gabridine eluted.

≪ Experimental Example 2 > Evaluation of endurance enhancement efficacy in obese animals

The effect of licorice extract extracts on the weight and exercise performance of obese animals was investigated in order to develop a material having both anti - obesity effect and exercise performance increasing ability derived from licorice. Four weeks old mice were purchased, and after 1 week of adaptation, the experimental diet was fed for 4 weeks, divided into the saline group and the high fat diet group. As a result of the 4-week high-fat dietary diet, we tried to eliminate the differences in the characteristics of the experimental animals by eliminating the mice with high fat diet-induced body weight increase.

After selection, 10 rats were divided into 6 groups and fed the experimental diet for 9 weeks. Group 6 consisted of two groups: normal diet (N), exercise group fed high fat diet (CE), exercise group (EE) containing 1% of licorice ethanol extract and high fat diet containing licorice hot water extract (SL-E, SH-E, respectively) fed a high fat diet containing 0.1% or 0.25% of licorice supercritical extract.

Treadmill exercise was performed at 3, 6, and 9 weeks after the start of the experiment. 15 ㅀ The ramp training was carried out for 10 minutes at 10 m / min for 10 minutes at a rate of 5 m / min on the second day for 15 minutes, 10 m / min for 5 minutes and 15 minutes for the first day at a speed of 5 m / min. After the exercise, the speed was increased to 1 m / min every 2 minutes. The maximal exercise performance was converted to the running distance from the grid to the stay for more than 10 seconds after stopping the exercise.

(A) Weight change and dietary intake

The body weight changes during the 9-week experimental period were measured weekly, showing a significant decrease in weight in the W-E, SL-E and SH-E groups (FIG. 3).

Comparing the body weight gain during the 9-week experimental period, the NE group increased by 7.8 g (0.85 g compared to the non-exercise group) while the CE group increased by 14.02 g (0.1 g compared to the non-exercise group). WE and SH-E groups significantly decreased weight gain to 8.96g and 6.86g, respectively. The SH-E group showed the highest weight loss effect by exercise compared to 8.76g and 9.14 of non-exercise group 4). However, the dietary intake during the experimental period did not show any difference between the experimental groups (Fig. 5), suggesting that the dietary intake was not the weight gain due to the decreased intake.

(B) Maximum exercise performance

In order to measure the effect of the licorice extract on the exercise performance of obese animals, the empirical formula was calculated as distance to exhaustion after 3, 6, and 9 weeks of treadmill exercise, Respectively. As a result, the exercise performance was increased in EE, WE and SH-E after 6 weeks of feeding and in WE and SH-E groups in 9 weeks. Especially, in SH-E group of 9 weeks, 2.3 times higher than that of NE group and 1.3 times higher than that of NE group (Figure 6).

(C) Organ weight

The results of the 9-week empirical formula showed that the weight of the liver due to high fat dietary supplementation was higher than that of all the licorice extract (Fig. 7) Group significantly decreased. In the case of epididymal fat and peripheral fat of white fat, WE and SH-E groups were significantly lower than CE. In the case of brown fat, all of the licorice extract group showed a statistically significant decrease.

(D) Histological analysis

H & E stain of formalin fixed tissue sections for liver, epididymal fat (WAT), and brown fat (BAT) tissues showed a tendency to be observed in liver, weaning, , The fatty liver lesions were significantly inhibited compared to the high fat diet alone group. In the case of epididymal fat, the size of adipocyte was decreased when the licorice extract was fed, and in the case of brown fat, it was confirmed that the decrease in tissue weight was due to inhibition of intracellular fat deposition (FIG. 8).

(E) Blood lipid changes

A significant increase in TG, TC, HDL-C and LDL-C was observed in the CE group compared to NE (Fig. 9), when the effect of the licorice extract on the lipid content of the blood was measured TG was significantly decreased in the WE and SL-E groups, but TC, LDL-C and NEFA were significantly decreased in all the licorice extract-fed groups, suggesting that the lipid-modulating effect was high.

(F) Blood sugar, serum hormone and enzyme activity

There was no difference in blood glucose between groups in the blood, which is considered to be due to blood collection after evaluation of maximal exercise performance without fasting. Serum leptin was significantly decreased in the licorice extract-treated group, especially in the SH-E group, which had excellent endurance-enhancing effects. If glycogen or glucose is decomposed and ATP is not supplied enough oxygen in the cell, the accumulation of lactic acid as a result of anaerobic metabolism will increase the activity of LDH, and LDH is a representative marker of muscle damage. Serum LDH was significantly increased in the C-E group compared with the N-E group, and all of them were effectively inhibited in the licorice extract-fed group. In other words, it seems to be protective against muscle damage induced by endurance exercise when licorice extract is fed. The antioxidant enzyme, SOD, tended to increase in all groups fed the licorice extract compared to the C-E group, but not significantly (Table 2).

Changes in blood glucose, hormone and enzyme activity Glucose (ng / ml) Leptin (ng / ml) LDH (IU / L) SOD (U / min / mg protein) N-E 241.6 ± 7.9 15.76 + - 0.82 60.64 ± 3.77 2.87 ± 0.44 C-E 284.2 ± 15.1 61.83 + - 0.81 107.60 ± 1.73 1.89 ± 0.18 E-E 269.9 ± 7.5 43.81 + - 0.91 68.85 ± 1.77 4.35 ± 1.13 W-E 249.2 ± 10.9 30.05 ± 3.46 59.58 ± 1.39 2.54 + 0.24 SL-E 253.7 ± 12.1 43.56 ± 3.82 82.79 + 5.08 2.17 ± 0.33 SH-E 257.8 ± 8.5 21.99 ± 2.60 81.66 + 5.06 4.50 + - 0.81

(G) Liver lipid changes

Hepatic total lipid, hepatic TG, hepatic TG and NEFA showed that the licorice extract significantly inhibited the elevation of blood lipid levels by high fat diet (Table 10) ).

(A) movement marker change between

CPK, an enzyme that catalyzes the synthesis of creatine phosphate required for the re-synthesis of ATP during anaerobic exercise, is also known as a marker of muscle damage. The CPK increased in the CE group significantly decreased in the licorice extract group. In the case of liver glycogen, a significantly higher level in the CE group is due to the fact that the excess energy is stored in the form of glycogen in the liver due to the high fat diet and developed into fatty liver lesions. In addition, the CE group, which performed a relatively small amount of exercise after the maximal exercise, was considered to be one of the reasons for not using much glycogen in the liver (Fig. 11).

(A) Movement marker of muscle

The concentration of lactate and glycogen in the muscle was measured after maximal exercise (Fig. 12). Lactic acid is a fatigue substance that accumulates in myocytes during anaerobic exercise. When accumulated in the body by exercise, it induces the inhibition of your life which becomes a source of kinetic energy through acidification of the body environment. In the C-E group, accumulation of lactic acid in the muscle was increased, but accumulation of lactic acid in the exercise group with the licorice extract was decreased.

The concentration of glycogen in muscles is known to be a factor affecting endurance exercise performance. In this experiment, the concentration of glycogen in muscle was decreased rather than that of CE group. This was because the exercise distance until the burnout was significantly shorter in the CE group than in the other experimental groups, suggesting that the glycogen in the muscle was depleted relatively.

Muscle fiber type transition

To investigate the post-exercise changes of the gastrocnemius muscles, which are the representative muscles of fast and glycolytic type II muscles, troponin I, a slow and aerobic type I muscle marker, and MyHCIIb, a fasting marker, (Fig. 13). As a result, the fasting tendency was decreased and the root was increased in the licorice extract-fed group (Fig. 13).

(K) Changes in volume and respiration rate

In order to investigate the effect of respiratory rate on weight change, VO ₂ , VCO ₂ and respiratory quotient (RQ) were measured by calorimetry for 12 hours from 7:00 PM to 7:00 AM in each group, same. The N group tended to have a higher RQ than the C group, but it was not significant. For the WE group, VO ₂ and VCO ₂ All were increased and RQ was maintained. The SL and SH groups also include VO ₂ and VCO ₂ All showed a tendency to increase but no significant difference.

<Experimental Example 3>

The composition for improving endurance or athletic performance (A) of the present invention comprising 5% by weight of hot water extract and 5% by weight of hot water extract of licorice was treated with mature fat cells for 48 hours and then dyed with DAPI to induce apoptosis induction of adipocytes As a result of the evaluation, it was confirmed that the apoptosis of the adipocyte was increased as compared with the composition for improving endurance or athletic performance (C) containing 10% by weight of the liquorice hot water extract containing no hot water extract (Fig. 15).

While the present invention has been particularly shown and described with reference to exemplary embodiments thereof, it will be understood by those skilled in the art that various changes and modifications may be made without departing from the spirit and scope of the invention as defined in the appended claims. It can be understood that

Claims (1)

A composition for inhibiting obesity comprising an extract of licorice and an extract of an extract as an active ingredient.

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