KR101401788B1 - Method of processing roots of wild ginseng - Google Patents

Abstract

The present invention relates to a novel processing method of a ginseng culturing muscle having an increased capacity of Rg3, Rh2 saponin and a purple wild ginseng culture machine processed by the above method. Rg3, Rh2 ginseng saponins as well as providing (ginsenoside) increased ginseng baeyanggeun hundredfold amount to more Rg3, Rh2 content of ginseng baeyanggeun unprocessed of Rg3, Rh2, as well as Rg1, Rh1, Rf other saponins than ginseng saponin (Crude saponin) also increased more than 10 times. Therefore, the wild ginseng cultured muscle prepared in the present invention is expected to be useful in the field of inhibiting cancer cell proliferation, inhibiting cancer metastasis, enhancing the anticancer activity of anticancer drugs, and treating cancer, wherein ginsenoside Rg3 and Rh2 are effective. This is because high amounts of Rg3 and Rh2 can be produced in large quantities.

Description

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a method for producing purple ginseng cultured muscle,

The present invention relates to a novel processing method for increasing the specific saponin capacity of the wild ginseng root.

Ginseng ( Panax schinseng NESS) is similar to ginseng but is formed by a slightly different gene. The pharmacological effect of wild ginseng is far superior to that of ginseng, but its research and use are limited because of its rarity. Recently, however, it has become possible to cultivate wild ginseng cells by studying cell cultivation techniques and to study or use wild ginseng in the public.

The active ingredient of ginseng is known as ginsenoside, and its main pharmacological effects are immune enhancement, antidiabetic action, fatigue recovery, antistress action, brain activity promotion effect, nerve cell protection action, blood circulation stimulation action, sexual function Various physiological activities such as the improvement of the disorder, the inhibition of the central nervous system, the detoxifying action, the inhibition of platelet aggregation, and especially the anti-cancer and cancer treatment are known.

On the other hand, the processing of such ginseng generally increases the content of ginsenoside. The processing method of ginseng is generally repeatedly rinsing and drying several times under a high temperature condition of 100 캜 or more. In this case, however, not only the steaming and drying process must be repeated several times, but also the severe production conditions must be continuously satisfied, resulting in a decrease in the productivity and economy of the production process. In addition, there is a problem that the content of the effective ginsenoside component contained in the processed ginseng is not substantially increased.

Disclosure of Invention Technical Problem [8] Accordingly, the present invention has been made in order to solve the above-mentioned problems, and it is an object of the present invention to provide a novel preparation method of purple ginseng cultured ginseng which can obtain various kinds of ginseng saponin .

In the present invention, dried ginseng root or powder thereof is put into a sealed high-pressure steam boiler, water of about 1/50 to 1/100 of the volume of the vessel provided in the steam boiler is added, Wherein the content of the ginsenosides Rg3 and Rh2 is increased, including a first step (i) of heating the mixture at 80 to 100 DEG C for 10 to 48 hours under a nitrogen atmosphere.

It is preferable that the manufacturing method further includes a second step (ii) of heating and drying at 100 to 500 占 폚 for 10 to 48 hours under the condition of the high pressure condition of the first step (i).

Here, the method may further include performing the first step or the second step while maintaining the condition of the step (i), and then repeating the first step, the second step, or the first step and the second step, It is preferable to carry out 10 times repeatedly and dry.

In addition, the dried ginseng root or powder thereof is added to a sealed high-pressure steam boiler, water of about 1/50 to 1/100 of the volume of the vessel provided in the steam boiler is added, And the second step (ii) in which the ginsenosides Rg3 and Rh2 are heated for 10 to 48 hours and then dried are repeated 1 to 10 times to increase the content of ginsenosides Rg3 and Rh2.

Here, it is preferable that the high-pressure steamer is provided with a safety pin for maintaining pressure in order to prevent breakage of the container.

Preferably, the dry ginseng culture muscle has a water content of 5% or less.

In the first and second steps, the temperature range, the volume of the water to be introduced, and the high pressure range are not limited by the above-described range, and can be variously adjusted according to the purpose.

In addition, the present invention provides a purple wild ginseng cultured muscle prepared by the above method. As shown in the attached chart, the prepared ginseng cultured root was increased about 300 times as much as that of wild ginseng cultured root in Rg3 and mass production of various kinds of ginseng saponin could be achieved by controlling temperature and pressure.

The present invention can provide a novel method for producing a purple oriental ginseng culture muscle in which the content of a specific ginsenoside is significantly increased while improving the productivity and economy of the production process.

The cultured ginseng cultured muscle according to the present invention contains a greater variety of saponins and higher contents thereof than general ginseng culture roots or wild ginseng culture roots processed by other conventional methods. Therefore, when a health supplement containing the wild ginseng root or the wild ginseng root is administered, various pharmacological effects can be exhibited due to a high content of Rg3 (cancer cell transplantation inhibitor) and Rh2 (cancer treatment agent).

Fig. 1 is a table showing the compositional analysis results of the first and second steps in Example 1. Fig.
FIG. 2 is a component analysis test chart in which the steps 1 and 2 in Example 2 were carried out.
FIG. 3 is a component analysis test chart obtained by repeating Step 1 and Step 2 in Example 3.
Fig. 4 is a component analysis test chart of Example 4, which was conducted for only one step for a long time.
FIG. 5 is a compositional analysis chart of a pure wild ginseng cultured muscle that has not undergone steps 1 and 2.

Hereinafter, the present invention will be described in detail.

The present invention is characterized in that the ginseng cultivation roots are processed with steam of high pressure and high temperature instead of processing the ginseng by repeatedly mulching at several times under atmospheric pressure conditions.

Through this processing method, in the present invention, it is possible to hydrolyze a conventional ginsenoside (for example, Rg1) contained in the cultured ginseng root and to synthesize a ginsenoside of another effective ingredient by using the degraded ginsenoside and the culture root itself , The content of specific ginsenosides (e.g., Rg3, Rh2, crude saponin, etc.) can be significantly increased (see Table 1 and Figs. 1 to 5 below).

For example, the dried ginseng culture muscle and water are put into a high-pressure steam boiler, and heated and dried while maintaining a high pressure state as much as possible. The following three methods can be exemplified as a more preferable embodiment. However, the present invention is not limited to the following method.

1) First Embodiment

The first embodiment of the method for manufacturing the wild ginseng culture muscle according to the present invention is characterized in that the dried wild ginseng culture roots or their powder are put into a sealed high pressure steam boiler and the steam volume of about 1/50 to 1/100 After the addition of water, the mixture is first heated for 10 to 48 hours at 80 to 100 占 폚 under a high-pressure condition of 1.5 to 2 atm.

The cultured roots of ginseng used in the present invention are not particularly limited as long as they are cultured roots of ginseng formed by culturing wild ginseng under specific conditions. Any of the wild ginseng culture roots itself and the wild ginseng culture roots known in the art can be used Includes wild ginseng and cultivated wild ginseng.

The source of wild ginseng used in the tissue culture of the wild ginseng culture muscle is not particularly limited, and examples thereof include domestic, American, Japanese, Himalayan, Vietnamese and Chinese.

Non-limiting examples of usable ginseng culture roots include a ginseng culture root body, a ginseng culture root powder, and a wild ginseng culture root concentrate. In the present invention, the ginseng cultured muscle powder is mainly used, but any of commercially available white ginseng, fresh ginseng, red ginseng, and the like may be used in the practice of the present invention.

At this time, if the ginseng culture muscle contains a large amount of water, it is difficult to pressurize the above-mentioned high pressure condition, and therefore, it is preferable to dry it. For example, it is preferred that the dried mountain ginseng cultured muscle has a moisture content of 5% or less in itself.

The use amount of the wild ginseng culture muscle is not particularly limited, and it is preferable to use it in a range of 1/10 or less based on the volume of the container.

The high-pressure steamer according to the present invention refers to a device for steaming a wild ginseng culture muscle under a specific pressure condition. At this time, the high-pressure steamer has a vessel capable of accommodating the ginseng cultivation muscle under a specific high-pressure condition. Non-limiting examples of vessels that can be used include silver vessels, earthen vessels, glass vessels, ceramic vessels, and non-toxic metal vessels. It is preferable to use a container of earthenware having a large germanium content.

The water used together with the wild ginseng cultivation muscle is preferably in a range of 1/50 to 1/10 of the volume of the container into which the wild ginseng culture muscle is introduced in consideration of the moisture absorbed by the dried ginseng cultivating muscle in the container while preventing the water vapor pressure in the high- / 100 can be used. By adjusting the heating temperature in the sealed container in this way, the pressure due to the water vapor pressure in the container can be controlled.

In addition, the pressurizing condition in the high pressure steam boiler in the first step is not particularly limited, and it is effective to use the maximum steam pressure obtained by heating to 100 ° C or less, preferably 80 to 95 ° C. At this time, it is necessary to install a safety pin to prevent breakage of the high pressure steam boiler.

The heating and drying conditions in the first step can be heated to 100 ° C or less, preferably 80 to 100 ° C for 10 to 48 hours. At this time, it is preferable to heat the ginseng saponin for a long time in order to sufficiently obtain saponin of ginseng, so that it is not limited to the above range. The reason why the temperature is heated to 100 ° C or less is to sufficiently obtain ginseng saponin produced at 100 ° C or less.

2) Embodiment 2

The second embodiment of the method for producing a wild ginseng culture muscle according to the present invention is to heat a wild ginseng culture muscle under a high pressure / high temperature condition higher than that of the above first embodiment. For example, the dried ginseng root or powder thereof is put into a sealed high-pressure steam boiler, water of about 1/50 to 1/100 of the volume of the vessel provided in the steam boiler is added, And then heated to a temperature of 100 ° C or higher for 10 to 48 hours and dried (second step).

The heating conditions of the second embodiment may be, for example, 100 ° C or higher, preferably 100 to 500 ° C for 10 to 48 hours. As in the first step, in order to obtain ginseng saponin sufficiently, it is preferable to heat the ginseng saponin for a long period of time, so that it is not limited to the above range.

When the temperature is raised to 100 ° C or higher in the sealed high pressure steam boiler, the internal pressure of the steam boiler due to the steam pressure is significantly increased. Therefore, the decompression of the existing ginsenoside and the decomposition of the new ginsenoside Side synthesis can be significantly increased. Here, since the pressure rises proportionally to the temperature, the above-described high-pressure range is not particularly limited. For example, it is preferable that the high-pressure range of the second embodiment increases the pressure of water vapor as much as possible within a range that the container can withstand. Other manufacturing conditions may be applied or modified as in the first embodiment.

In this case, when the second embodiment is carried out first or independently, the third step (iii) is carried out in a state of maintaining the high temperature / high pressure condition of the second embodiment (ii) at a temperature lower than that of the second embodiment ). For example, the third step may be performed by heating at a temperature of 90 to 110 ° C for 10 to 48 hours.

3) Third Embodiment

The third embodiment of the method for producing the wild ginseng root according to the present invention is to sequentially perform the first embodiment (the first step) and the second embodiment (the second step). For example, after completing the first step, the cultured ginseng cultured muscle may be heated and dried under the high pressure condition of the first step. Or the first step or the second step is carried out while maintaining the condition of the first step (i), and then the first step, the second step, or the first step and the second step are repeated several times Followed by drying.

Herein, as the number of repetitions increases, the kinds and contents of the effective ginsenosides in the wild ginseng culture muscle are increased. Therefore, the number and order of repetition of the first and second steps described above are not particularly limited. For example, the repetition number may be 1 to 10 times.

In the first to third embodiments according to the present invention, the temperature range, the volume of the water to be introduced, and the pressure range are not limited by the above-mentioned range, and may be set within a range in which the container provided in the high- It is also within the scope of the present invention to apply a temperature and pressure range higher than the above-mentioned range.

Meanwhile, although the first and second steps are described in the present invention, the first and second steps may be repeated one or more times, for example, about 1 to 10 times, if necessary. It is also within the scope of the present invention to appropriately change the order of the first step and the second step according to the purpose.

When the preparation step is performed as described above, the wild ginseng cultured muscle exhibits a deep purple color.

In the present invention, saponin, which is the main component of the wild ginseng root, is best attracted and can contain more kinds of saponin than other wild ginsengs. Herein, the term "saponin " in the present invention means ginsenoside, i.e. ginsenoside, unless otherwise specified, and saponin of other plants may be variously increased in the same manner.

In particular, the wild ginseng culture root produced according to the present invention exhibits a content of ginsenoside components and contents different from those of the wild ginseng culture root.

For example, the content of ginsenoside Rg1 is high while the content of ginsenoside Rg3 and Rh2 is extremely low. On the other hand, in the present invention, it was confirmed that the content of ginsenoside Rg1 in the processed wild ginseng culture muscle was almost zero, and the content of ginsenoside Rg3 and Rh2 increased several hundreds of times (see Table 1). This indicates that the components contained in the wild ginseng root and the existing ginseng saponin were hydrolyzed or synthesized through the novel production method of the present invention to increase the higher quality saponins such as new ginsenosides Rg3 and Rh2.

Therefore, the wild ginseng cultured muscle prepared in the present invention can inhibit the platelet aggregation inhibitory action of ginsenoside Rg3 and Rh2, inhibit cancer cell proliferation, inhibit metastasis of cancer, increase anticancer activity of anti-cancer drug, anti-allergic effect, anti- It is expected that it will be useful in the therapeutic field because the mass production of Rg3 and Rh2 is possible.

The ginseng cultured muscle prepared according to the present invention can be used in the form of a solution which is formed by pulverization, pulverization, freezing, or vacuum drying by pulverization or immersion in a drinking water according to a conventional method, and can be used as an injection. Available. Or the powder or the solution may be added to medicines, foods, healthcare aids and the like.

The ginseng cultured muscle prepared according to the present invention can be used as a medicine for anti-cancer, anti-inflammation, antiallergic and the like.

When the wild ginseng root of the present invention is used as a medicament for cancer, anti-inflammation or antiallergic use, it may further include a conventional carrier, excipient or diluent known in the art if necessary.

In addition, the wild ginseng root prepared according to the present invention can be used as a health functional food.

Hereinafter, the present invention will be described in detail with reference to examples. However, these examples are for illustrating the present invention specifically, and the scope of the present invention is not limited to these examples.

Example 1

1-1. Dried ginseng cultivation muscle

The dried ginseng cultivation muscle was harvested, washed three times with water, spread to a thickness of 3 to 5 cm, dried in an automatic drier, dried at 80 ° C. for 9 hours to have a water content of 5% or less, .

1-2. Manufacture of wild ginseng root

500 g of the dried ginseng cultivation muscle dried in Example 1-1 was placed in a sterilized silver container (10 L) in a high- Water having a volume of about 1/100 of the volume of the container was placed in the vessel, and a safety pin was attached so that the water pressure did not rise above 2 atm. Then, the vessel was firstly heated at 95 ° C for 24 hours.

Thereafter, the temperature was raised to 130 캜 while the pressure was maintained, and then the secondary was heated for 24 hours. The heated ginseng culture roots were spread to a thickness of 1 ~ 2 cm in a drying tray and dried at 90 ~ 100 ℃ for 10 hours. The results are shown in Table 1 below. [Table 1] < tb >< TABLE >

[Examples 2 to 3]

The same procedure as in Example 1 was carried out to produce a ginseng cultured muscle.

[Example 4]

The same procedure as in Example 1 was conducted except that the first heating step and the drying step were carried out without performing the second heating step, thereby producing a ginseng culture muscle.

EXPERIMENTAL EXAMPLES 1. Comparison of saponin components of cultured ginseng cultured muscle and saponin components of cultured ginseng cultured muscle

The ginseng cultured muscles prepared in Examples 1 to 4 were analyzed by a component analysis at the Life Science Research Center, Chungbuk National University Industrial Research & Development Cooperation Agency (Designated as Food Hygiene Inspection Agency No.38). The analytical results of the manufactured ginseng cultured muscle are shown in Table 1 below.

At this time, Examples 1 to 3 were heated and matured under the conditions of high temperature and high pressure of the first and second stages, and Example 4 was heated and steamed under the conditions of high temperature and high pressure of the first stage under different conditions.

As shown in Table 1, it can be seen that the wild ginseng culture roots prepared in Examples 1 to 4 contain higher levels of saponins and their contents than the untreated wild ginseng culture roots. In particular, it was confirmed that the content of specific ginsenosides such as Rg3, Rh2 and the like in the wild ginseng culture roots of Examples 1 to 3 processed under high temperature / high pressure conditions was significantly increased.

Therefore, the method of the present invention for the preparation of the wild ginseng root can be applied to various fields requiring various pharmacological effects.

Test items and results (mg / g) Manufacturing conditions Rg1 Rf Rg2 Rh1 Rg3 Rh2 Crude saponin Step 1 Step 2 Example 1
Wild ginseng cultivator 0 0.205 0.046 0.251 6.42 1.787 50.10 ○ ○ Example 2
Wild ginseng cultivator 0 0.119 0.192 0.262 4.07 1.233 65.1 ○ ○ Example 3
Wild ginseng cultivator 0.045 0.047 0.104 0.134 4.77 3.466 96.5 ○ ○ Example 4
Wild ginseng cultivator 0.155 0.012 0.047 0.00 2.28 0.658 78.7 ○ × Untreated
Wild ginseng cultivator 0.82 0.27 0.28 0.02 0.02 7.37 × ×

As shown in Table 1, it can be seen that the wild ginseng cultured muscle prepared in Examples 1 to 4 contains higher levels of saponins of Rg3 and Rh2 than untreated wild ginseng cultured ginseng. In particular, it can be confirmed that the content of ginseng saponin (ginsenoside) of Rg3 and Rh2 in the wild ginseng root of Examples 1 to 3 was greatly increased.

Claims (5)

The dried ginseng root or powder thereof is put into a sealed high-pressure steam boiler, water of about 1/50 to 1/100 of the volume of the vessel provided in the booster is added, and then a safety pin And a first step of heating the mixture at 80 to 100 DEG C for 10 to 48 hours to increase the content of ginsenosides Rg3 and Rh2.
2. The method according to claim 1, further comprising a second step of heating at 100 to 500 DEG C for 10 to 48 hours while maintaining the high-pressure condition of step 1 of claim 1
delete delete A purple wild ginseng culture root produced by the method according to any one of claims 1 to 2.

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