CN107136370A - A kind of herb fermenting beverage with antitumor efficacy and preparation method thereof - Google Patents

A kind of herb fermenting beverage with antitumor efficacy and preparation method thereof Download PDF

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CN107136370A
CN107136370A CN201710319498.1A CN201710319498A CN107136370A CN 107136370 A CN107136370 A CN 107136370A CN 201710319498 A CN201710319498 A CN 201710319498A CN 107136370 A CN107136370 A CN 107136370A
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acid bacteria
cfu
honeysuckle
fermented beverage
extract
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王宝林
郭春荣
张春杰
穆文娟
杨华
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TIANJIN QIJUN BIOTECHNOLOGY CO Ltd
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TIANJIN QIJUN BIOTECHNOLOGY CO Ltd
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Abstract

The invention provides a kind of herb fermenting beverage with antitumor efficacy and preparation method thereof, by matrimony vine honeysuckle, extract, liquid glucose, lactic acid bacteria, saccharomycete and acetic acid bacteria are constituted the active ingredient of the beverage altogether, wherein, there are liquid glucose 300mL, lactic acid bacteria 1 × 10 in common extract per 500mL matrimony vines honeysuckle3‑1×1013CFU, saccharomycete 1 × 103‑1×1013CFU, acetic acid bacteria 1 × 103‑1×1013CFU, lactic acid bacteria, saccharomycete and acetic acid bacteria are added and prepared in matrimony vine honeysuckle common extract, liquid glucose mixed liquor, gained beverage is proved through in vitro test, notable to HT29 (human colon cancer cell strain) S180 (strain of mouse hydroperitoneum type sarcoma cell) external 72h inhibiting rates of HpG2 (human hepatoma cell strain).

Description

Traditional Chinese medicine fermented beverage with anti-tumor effect and preparation method thereof
Technical Field
The invention relates to a fermented beverage and a preparation method thereof, in particular to a traditional Chinese medicine fermented beverage with an anti-tumor effect and a preparation method thereof.
Background
Malignant tumor is a disease caused by the over proliferation of cells in the body due to the loss of normal regulation and control. Hyperproliferative cells are called cancer cells, which often invade surrounding tissues (infiltration, i nvas ion) and may even be transferred to other parts of the body via the systemic circulation and/or the lymphatic system (cancer metastasis). There are many types of cancer, and the severity of the condition depends on the location of the cancer cells, the degree of malignancy, and whether metastasis has occurred. Once diagnosed, treatment is often performed in combination with surgery, chemotherapy, and radiation therapy. If the cancer is untreated, the end result will often lead to death.
At present, the traditional therapy for malignant tumor mainly adopts radiotherapy and chemotherapy, and the traditional chemotherapy has a plurality of side effects, including immunosuppression, mucosal injury and the like, which directly affect the life quality of patients, so that the substitute of the chemotherapy is urgently needed to be searched.
The anti-tumor traditional Chinese medicine is mainly summarized into two methods of eliminating pathogenic factors and strengthening body resistance, namely a method of eliminating pathogenic factors, clearing away heat and toxic materials, activating blood and removing stasis, eliminating phlegm and resolving masses and a method of combating poison with poison; the methods of strengthening the body resistance, which are based on the orientation of the excess and deficiency of yin, yang, qi, blood, viscera and meridians, are classified into four methods of invigorating spleen, nourishing blood, tonifying kidney and nourishing yin. Mainly takes syndrome differentiation treatment as a main treatment, and can effectively control tumor development, improve survival time and improve survival quality. Many herbal medicines contain anti-tumor components and inhibit or kill tumor cells through multiple pathways and multiple targets. Research suggests that the anticancer mechanism of traditional Chinese medicine mainly has the following aspects: the traditional Chinese medicine has the advantages of relatively small side effect, can prevent cancer cells from relapse and transferring and improve the immunity of patients, so the traditional Chinese medicine is more and more widely applied to clinical application.
Disclosure of Invention
The invention aims to provide a traditional Chinese medicine fermented beverage with an extremely high suspension rate in water and an anti-tumor effect.
The invention also aims to provide a preparation method of the traditional Chinese medicine fermented beverage with the anti-tumor effect.
In order to solve the technical problems, the technical scheme of the invention is as follows:
a Chinese medicinal fermented beverage with anti-tumor effect comprises fructus Lycii and flos Lonicerae extract, sugar solution, lactobacillus, yeast and acetic acid bacteria as effective components, wherein each 500mL of fructus Lycii and flos Lonicerae extract contains 300mL of sugar solution and lactobacillus 1 × 103-1×1013CFU, Yeast 1 × 103-1×1013CFU, Acetobacter xylinum 1 × 103-1×1013CFU。
Preferably, the Chinese medicinal fermented beverage with the anti-tumor effect is prepared by the following steps: mixing 10-80g of dried fructus Lycii and 10-80g of flos Lonicerae, adding 500mL of purified water, extracting at 70-100 deg.C for 10-120min, filtering with 40 mesh sieve, extracting the residue with purified water at 70-100 deg.C for 10-120min, filtering with 40 mesh sieve, and mixing the two filtrates together.
Preferably, the sugar solution of the fermented beverage with anti-tumor effect is prepared by the following steps: mixing 10-100g of white granulated sugar and 10-100g of honey, adding 300mL of purified water, dissolving, and filtering to obtain the final product.
Preferably, in every 500mL of the Chinese medicinal fermented beverage with the anti-tumor effect, 300mL of sugar solution and 1 × 10 mL of lactic acid bacteria are contained in the Chinese medicinal fermented beverage11CFU, Yeast 1 × 109CFU, Acetobacter xylinum 1 × 1010CFU。
Preferably, the weight ratio of the dried medlar to the honeysuckle is 7: 3.
The preparation method of the traditional Chinese medicine fermented beverage with the anti-tumor effect comprises the following specific steps:
(1) mixing the extracts of fructus Lycii and flos Lonicerae with sugar solution, adding purified water, balancing to 1L, sterilizing at 121 deg.C for 20min, cooling to room temperature, inoculating lactobacillus, yeast and acetic acid bacteria, fermenting at 20-40 deg.C for 3-15 d;
(2) the fermentation broth was centrifuged under sterile conditions: centrifuging at 10000rpm for 5-20min at 2000-.
The invention has the beneficial effects that:
the in vitro test proves that the traditional Chinese medicine fermented beverage with the anti-tumor effect has 78.56%, 82.13% and 68.38% inhibition rates for 72h in vitro of HT29 (human colon cancer cell line) S180 (mouse ascites type sarcoma cell line) HpG2 (human liver cancer cell line); the traditional Chinese medicine fermented beverage in an S180 cell tumor-bearing mouse experiment can improve the micro-ecological environment in a mouse body, enhance the immunity of the mouse and effectively inhibit the growth of cancer cells in an animal body, and the inhibition rate of the traditional Chinese medicine fermented beverage reaches 46.1%. And can improve the food intake, weight and mental state of the mice.
Therefore, the traditional Chinese medicine fermented beverage disclosed by the invention can effectively inhibit tumor growth through the synergistic effect of the components, meanwhile, the beverage is sour and sweet in taste and good in palatability, abnormal death or other adverse conditions of animals are not found in the animal experiment process, the product is high in safety and free of toxic and side effects, and is expected to replace or partially replace chemotherapeutic drugs clinically, the dosage of the chemotherapeutic drugs is reduced, the survival quality of patients is improved, a large amount of medical expenses are saved, the preparation method is simple, and the traditional Chinese medicine fermented beverage has a wide application prospect.
Drawings
FIG. 1 is a graph of the inhibitory effect of fermented beverages and aqueous extracts on HT29 cells;
FIG. 2 is a graph of the inhibitory effect of fermented beverages and aqueous extracts on S180 cells;
FIG. 3 is a graph of the inhibitory effect of fermented beverages and aqueous extracts on HepG-2 cells;
FIG. 4 is a graph of the effect of fermented beverages on mouse food intake;
FIG. 5 is a graph of the effect of fermented beverages on body weight in mice.
Detailed Description
The technical solution of the present invention is further described with reference to the following specific examples.
Example 1
A Chinese medicinal fermented beverage with anti-tumor effect comprises fructus Lycii flos Lonicerae co-extract, sugar solution, lactobacillus (Lactobacillus plantarum LP45, Shijiazhuang Yiran Biotech limited), yeast (fermented Pichia pastoris, CICC20442, China Industrial microbial strains preservation management center), and acetic acid bacteria (Acetobacter pasteurianus, CICC20064, China Industrial microbial strains preservation management center), wherein each 500mL of fructus Lycii flos Lonicerae co-extract contains 300mL of sugar solution, 1 st 1 × 10H 2H11CFU, Yeast 1 × 109CFU, Acetobacter xylinum 1 × 1010And (4) CFU. Wherein,
the medlar and honeysuckle co-extract is prepared by the following method: mixing 70g of dried fructus Lycii and 30g of flos Lonicerae, adding 500mL of purified water, leaching at 90 deg.C for 100min, filtering with 40 mesh sieve, leaching the residue with purified water at 80 deg.C for 80min, filtering with 40 mesh sieve, and mixing the two filtrates together.
The sugar solution is prepared by the following method: mixing 50g of white granulated sugar and 50g of honey, adding 300mL of purified water, dissolving, and filtering to clarify.
The preparation method of the traditional Chinese medicine fermented beverage with the anti-tumor effect comprises the following specific steps:
(1) mixing the extracts of fructus Lycii and flos Lonicerae with sugar solution, adding purified water, balancing to 1L, sterilizing at 121 deg.C for 20min, cooling to room temperature, inoculating lactobacillus, yeast and acetic acid bacteria, fermenting at 30 deg.C for 10 d;
(2) the fermentation broth was centrifuged under sterile conditions: centrifuging at 8000rpm for 15min, filtering the supernatant with 0.2 μm microporous membrane for sterilization, and packaging.
Example 2
A Chinese medicinal fermented beverage with anti-tumor effect comprises fructus Lycii and flos Lonicerae co-extract, sugar solution, lactobacillus (Lactobacillus plantarum LP45, Shijiazhuang Yiran Biotech Co., Ltd.), yeast (Saccharomyces bougainvilleae, CICC31994, China Industrial microbial strains preservation management center) and acetic acid bacteria (Acetobacter pasteurianus, CICC20056, China Industrial microbial strains preservation management center), wherein each 500mL of fructus Lycii and flos Lonicerae co-extract contains 300mL of sugar solution, 1 × 10 of lactobacillus3CFU, Yeast 1 × 1013CFU, Acetobacter xylinum 1 × 103And (4) CFU. Wherein,
the medlar and honeysuckle co-extract is prepared by the following method: mixing every 80g of dried fructus Lycii and 10g of flos Lonicerae, adding 500mL of purified water, leaching at 100 deg.C for 120min, filtering with 40 mesh sieve, leaching the residue with purified water at 70 deg.C for 10min, filtering with 40 mesh sieve, and mixing the two filtrates together.
The sugar solution is prepared by the following method: mixing every 10g of white granulated sugar and 100g of honey, adding 300mL of purified water, dissolving, and filtering to clarify.
The preparation method of the traditional Chinese medicine fermented beverage with the anti-tumor effect comprises the following specific steps:
(1) mixing the extracts of fructus Lycii and flos Lonicerae with sugar solution, adding purified water, balancing to 1L, sterilizing at 121 deg.C for 20min, cooling to room temperature, inoculating lactobacillus, yeast and acetic acid bacteria, fermenting at 20 deg.C for 15 d;
(2) the fermentation broth was centrifuged under sterile conditions: centrifuging at 10000rpm for 5min, filtering the supernatant with 0.2 μm microporous membrane for sterilization, and packaging.
Example 3
A Chinese medicinal fermented beverage with anti-tumor effect comprises fructus Lycii flos Lonicerae co-extract, sugar solution, lactobacillus (Lactobacillus plantarum LP-G18, Shanghai bioengineering (Shanghai) Co., Ltd.), yeast (Saccharomyces cerevisiae, CICC1002, China center for preservation and management of industrial microorganism strains) and acetic acid bacteria (Acetobacter pasteurianus, CGMCC1.24, China center for preservation and management of common microorganism strains), wherein each 500mL of fructus Lycii flos Lonicerae co-extract contains 300mL of sugar solution and 1 × 10 of lactobacillus13CFU, Yeast 1 × 103CFU, Acetobacter xylinum 1 × 1013And (4) CFU. Wherein,
the medlar and honeysuckle co-extract is prepared by the following method: mixing 10g of dried fructus Lycii and 80g of flos Lonicerae, adding 500mL of purified water, extracting at 70 deg.C for 10min, filtering with 40 mesh sieve, extracting the residue with purified water at 100 deg.C for 120min, filtering with 40 mesh sieve, and mixing the two filtrates together.
The sugar solution is prepared by the following method: mixing every 100g of white granulated sugar and 10g of honey, adding 300mL of purified water, dissolving, and filtering to clarify.
The preparation method of the traditional Chinese medicine fermented beverage with the anti-tumor effect comprises the following specific steps:
(1) mixing the extracts of fructus Lycii and flos Lonicerae with sugar solution, adding purified water, balancing to 1L, sterilizing at 121 deg.C for 20min, cooling to room temperature, inoculating lactobacillus, yeast and acetic acid bacteria, fermenting at 40 deg.C for 3 d;
(2) the fermentation broth was centrifuged under sterile conditions: centrifuging at 2000rpm for 20min, filtering the supernatant with 0.2 μm microporous membrane for sterilization, and packaging.
Example 4
A fermented beverage containing Chinese medicinal materials with anti-tumor effect comprises the effective components of fructus Lycii and flos Lonicerae extract, sugar solution, lactobacillus (Lactobacillus plantarum LP-G18, Shanghai bioengineering (Shanghai) Co., Ltd.), yeast (Pichia pastoris, CICC20442, China center for preservation and management of industrial microorganism strains) and acetic acid bacteria (gluconacetobacter hanthii, CGMCC1.1811, China center for preservation and management of common microorganism strains), wherein each 500mL of fructus Lycii and flos Lonicerae extract contains 300mL of sugar solution and 1 × 10 of lactobacillus12CFU, Yeast 1 × 107CFU, Acetobacter xylinum 1 × 109And (4) CFU. Wherein,
the medlar and honeysuckle co-extract is prepared by the following method: mixing every 60g of dried fructus Lycii and 40g of flos Lonicerae, adding 500mL of purified water, leaching at 80 deg.C for 60min, filtering with 40 mesh sieve, leaching the residue with purified water at 80 deg.C for 40min, filtering with 40 mesh sieve, and mixing the two filtrates together.
The sugar solution is prepared by the following method: mixing every 60g of white granulated sugar and 40g of honey, adding 300mL of purified water, dissolving, and filtering to clarify.
The preparation method of the traditional Chinese medicine fermented beverage with the anti-tumor efficacy is the same as that of the example 1.
Example 5
A Chinese medicinal fermented beverage with anti-tumor effect comprises fructus Lycii flos Lonicerae co-extract, sugar solution, lactobacillus (Lactobacillus plantarum LP-G18, Shanghai bioengineering (Shanghai) Co., Ltd.), yeast (Saccharomyces cerevisiae, CICC1002, China center for preservation and management of industrial microorganism strains) and acetic acid bacteria (apple bacillus aceti, CGMCC1.5116, China center for preservation and management of common microorganism strains), wherein each 500mL of fructus Lycii flos Lonicerae co-extract contains 300mL of sugar solution and 1 × 10 of lactobacillus9CFU, Yeast 1 × 105CFU, Acetobacter xylinum 1 × 107And (4) CFU. Wherein,
the medlar and honeysuckle co-extract is prepared by the following method: mixing every 70g of dried fructus Lycii and 20g of flos Lonicerae, adding 500mL of purified water, leaching at 90 deg.C for 60min, filtering with 40 mesh sieve, leaching the residue with purified water at 80 deg.C for 40min, filtering with 40 mesh sieve, and mixing the two filtrates together.
The sugar solution is prepared by the following method: mixing every 40g of white granulated sugar and 60g of honey, adding 300mL of purified water, dissolving, and filtering to clarify.
The preparation method of the fermented beverage with anti-tumor effect is the same as that of example 2.
The amount of each component in the above examples is increased or decreased according to the same ratio, and the obtained relationship of the weight parts of each component is within the protection scope of the present invention.
The formula analysis of the embodiment comprises the following steps:
fructus Lycii is neutral in nature and sweet in taste, has effects of nourishing yin, replenishing blood, nourishing liver and kidney, replenishing vital essence and improving eyesight, and is used for treating soreness and weakness of waist and knees, dizziness, tinnitus, and nocturnal emission caused by deficiency of liver and kidney and deficiency of essence and blood. The main active ingredients are as follows:
wolfberry polysaccharide: the lycium barbarum polysaccharide is a water-soluble polysaccharide, is the main active ingredient in lycium barbarum, has a relative molecular mass of 68-200, and becomes a hot point of research at home and abroad. The study on the immunoregulation and anti-tumor effects of lycium barbarum polysaccharides is the most. Many studies show that the lycium barbarum polysaccharide has the effects of promoting immunity, resisting aging, resisting tumors, eliminating free radicals, resisting fatigue, resisting radiation, protecting the liver, protecting and improving reproductive function and the like.
Betaine: the chemical name is 1-carboxyl-N, N, N-trimethyl glycine lactone, which is similar to amino acid in chemical structure and belongs to quaternary ammonium base material. Betaine is one of the main alkaloids in the fruits, leaves and stalks of Chinese wolfberry. The action of lycium barbarum on lipid metabolism or lipotropism is mainly due to the betaine content, which acts as a methyl donor in the body. The research on lycium barbarum betaine is limited to domestic aspects of content determination, extraction process and physiological action (salt tolerance enhancement) research on lycium barbarum plants, and few pharmacological and pharmacodynamic researches on lycium barbarum betaine are carried out.
Medlar pigment: the Chinese wolfberry pigment is various color-producing substances existing in Chinese wolfberry berries and is an important physiological active ingredient of Chinese wolfberry seeds. Mainly including carotene, lutein and other colored substances. The carotenoid contained in the medlar has very important medicinal value. Many studies have proved that the lycium barbarum seed pigment has the effects of improving the human body immunity, preventing and inhibiting tumors, preventing atherosclerosis and the like. Carotene is the main active component of Lycium barbarum pigment, and has important physiological functions of resisting oxidation and serving as the synthesis precursor of vitamin A.
Honeysuckle is a good medicine for clearing heat and removing toxicity. It is sweet and cold in nature and fragrant in nature, and sweet and cold in nature and clearing heat without damaging the stomach. It is fragrant and can eliminate pathogens. The chemical components mainly comprise organic acid, flavonoid, triterpenoid saponin, volatile oil and the like. The honeysuckle can disperse wind-heat and also can clear away heat and toxic material, and has obvious effect on various heat diseases such as heat generation, eruption, macula, sore and carbuncle due to heat toxin, sore throat and the like. The honeysuckle comprises the following main active components:
organic acids: chlorogenic acid compounds are main effective components of flos Lonicerae, and comprise chlorogenic acid (chlorochlorogenic acid) and isochlorogenic acid (isochlorogenic acid), wherein isochlorogenic acid is a mixture of 7 isomers, which are respectively 4, 5-dicaffeoylquinic acid, 3, 4-dicaffeoylquinic acid, 3, 5-dicaffeoylquinic acid, 1, 3-dicaffeoylquinic acid, 3-feruloylquinic acid, 4-feruloylquinic acid, 5-feruloylquinic acid, and other organic acids including caffeic acid and palmitic acid.
Luteolin (luteolin) is firstly separated from honeysuckle in flavonoid Zhanqitailang of 1949, loniceraside is separated from him in 1961, and 4 flavonoids are firstly separated from honeysuckle in Gaoyamine of 1995.
Triterpene saponin, wherein a novel triterpene saponin containing six glycosyl groups is separated from honeysuckle flower in 1990. In 1993, Maoqing and the like are also separated to obtain 3 triterpene saponins. In 1994, Chenmin et al isolated 2 new dicaffeoylquinic acid vinegar compounds. Three triterpenoid saponins are separated from Mongolian snakegourd fruit and the like.
Volatile oil is the effective component of honeysuckle. Separating out linalool, bisabolol, palmitic acid, dihydrogeraniol, methyl tetracosanate, ethyl octadecadienoate, ethyl palmitate, 1-dicyclohexyl and other components by gas chromatography-mass spectrometry. The honeysuckle flower and dried flower have large component difference. The fresh flower volatile oil component is mainly linalool and has a content of more than 14%, and the dry flower volatile oil component is mainly palmitic acid and generally accounts for more than 26%. 5-hydroxy 7, 4-dimethoxy flavone was first discovered from this plant by the enzyme chennge et al, 2002, and quercetin, loniceraside, oleanolic acid and daucosterol were first discovered from the flower buds of this plant. The pharmacological actions of honeysuckle flower are mainly as follows: antibacterial, antiviral, antiinflammatory, antipyretic, cholagogue, hepatoprotective, blood lipid reducing, and immunity improving effects.
In vivo experiments show that the antitumor capability of the aqueous extracts of the medlar and the honeysuckle is obviously improved after the aqueous extracts of the medlar and the honeysuckle are fermented by lactic acid bacteria, saccharomycetes and acetic acid bacteria.
Test example (Experimental group using the fermented beverage of the Chinese medicine described in example 1)
Tumor cell proliferation inhibition assay (MTT method)
HT29, S180 and HepG-2 cells in logarithmic growth phase are taken, and the cell concentration is adjusted to 2.0 × 10 by using RPM I-1640 medium containing 10% fetal calf serum4-1.0×105And (3) inoculating the cells/well into a 96-well cell culture plate, culturing the cells at the temperature of 37 ℃ and 5% CO2 in each well by 200 mu L, adding 20 mu L of filter-sterilized fermented beverage or aqueous extract into each well, observing the apoptosis conditions of the cells for 12 hours, 24 hours, 48 hours and 72 hours by adopting an MTT method, wherein the experimental results are shown in figures 1-3.
Tumor bearing mouse experiment
Experimental animals: 105 SPF Kunming mice with the weight of 20 +/-3 g
Preparation of tumor-bearing mice: after the Kunming mouse is inoculated with S180 ascites tumor for 4d, the abdomen of the mouse is expanded, cervical dislocation is killed, 75% ethanol is used for soaking for 5min for disinfection, an abdomen incision is made in a clean bench, the abdominal cavity is exposed, white or light yellow ascites is extracted by an injector, the ascites is diluted by 4-5 times by sterile physiological saline, and the mixture is slowly mixed uniformly for molding. Using inguinal inoculation: mice were exposed to the groin, sterilized with 75% alcohol, and the needle was inserted from 1cm below the groin at an angle of 30 degrees to the subcutaneous level, and each mouse was injected with 0.2mL of an ascites diluent.
The groups of mice and the treatment regimen are shown in table 1.
TABLE 1
Group of Mode of treatment
A: blank group (not)Make mould) Normal saline for gastric perfusion
B: negative control group Normal saline for gastric perfusion
C: positive control group Fluorouracil injection 0.2mL (20mg/kg bw)
D: high dose group Gavage fermented beverage stock solution 0.4mL
E: middle dose group Gavage fermented beverage 2 times diluent 0.4mL
F: low dose group Gavage fermented beverage 4 times diluent 0.4mL
G: aqueous extract group Gavage unfermented aqueous extract 0.4mL
Each group of 15 mice
The mice are treated every day from the next day after model building, the treatment is carried out for 7d totally, the food intake and the body weight of the mice are recorded every day, the mice are killed at the 9 th day, the complete tumor tissues are separated, weighed and recorded.
The experimental results are as follows:
effect of fermented beverages on food intake of mice:
as shown in fig. 4, it can be seen from the experimental results that: the food intake of the chemotherapy groups is the lowest and is obviously different from that of the normal group, so that the food intake of the mice is greatly influenced by chemotherapy, the food intake of the blank group is the highest, the food intake of the high-dose group is the highest, and the food intake of the medium-dose group and the low-dose group are sequentially reduced, but are obviously different from those of the chemotherapy groups and are higher than those of the negative control group. The water extract group had higher food intake than the chemotherapy group, but the difference was not significant. The experiment shows that the beverage can effectively improve the food intake of tumor-bearing mice.
Effect of fermented beverages on mouse body weight:
as shown in fig. 5, it can be seen from the experimental results that: the body weight of the mice in the chemotherapy group is obviously reduced, the body weight and the nutrition condition of the mice are greatly influenced by chemotherapy, the body weight of the blank group is the highest, the body weight of the high-dose group is next to that of the blank group, and the body weights of the medium-dose group and the low-dose group are sequentially reduced, but are obviously different from those of the chemotherapy group and higher than that of the negative control group. The weight of the water extract group is higher than that of the chemotherapy group, and the difference is obvious. The experiment shows that the beverage can effectively increase the body weight of tumor-bearing mice.
The effect of fermented beverages on tumor inhibition in mice is shown in table 2:
TABLE 2
Group of Number of Tumor weight (g) Inhibition ratio (%)
A: blank group 15 0 --
Negative control group (No treatment) 15 0.74±0.38 0
C: positive control group (Fluorouracil) 15 0.31±0.16 57.8
D: high dose group 15 0.40±0.22 46.1
E: middle dose group 15 0.54±0.29 27.1
F: low dose group 15 0.57±0.28 22.3
G: aqueous extract group 15 0.71±0.46 4.5
The experimental results show that: fluorouracil has the strongest inhibition to mouse tumor, the inhibition rate reaches 58.7%, the inhibition effect of high-dose group to mouse tumor is the second to 46.1%, the unfermented water extract group also has certain inhibition to mouse tumor, but only 4.5%.
The effect of fermented beverages on intestinal flora in mice is shown in table 3:
TABLE 3
The experimental results show that the intestinal flora of the mice is greatly changed after chemotherapy: the number of enterobacteria, enterococcus, lactobacillus and bifidobacterium is reduced obviously. The unfermented aqueous extract has certain recovery and protection effects on the mouse intestinal flora, and has obvious difference compared with the chemotherapy group. The high-dose group and the medium-dose group can effectively improve the number of various intestinal flora of mice, and are obviously different from the water extract group.
In vitro tests show that the inhibition rates of the traditional Chinese medicine fermented beverage on HT29 (human colon cancer cell line) S180 (mouse ascites type sarcoma cell line) HpG2 (human liver cancer cell line) for 72h in vitro are 78.56%, 82.13% and 68.38% respectively, and the inhibition rates of aqueous extracts are only as follows: 28.7%, 38.03%, 29.34%. Through S180 cell tumor-bearing mouse experiments, the product can improve the micro-ecological environment in a mouse body, enhance the immunity of the mouse and effectively inhibit the growth of cancer cells in the animal body, and the inhibition rate of the product reaches 46.1 percent. And can improve the food intake, weight and mental state of the mice.
In conclusion, the anti-tumor capability of the Chinese wolfberry and honeysuckle co-extract can be effectively improved after the mixed fermentation of acetic acid bacteria, lactic acid bacteria and saccharomycetes. Compared with the traditional chemotherapy method, the fermented beverage has the advantages that the tumor inhibition rate is far higher than that of the traditional Chinese medicine extract although being lower than that of fluorouracil chemotherapy, the oral acceptability is high, the animal nutrition condition and the ingestion condition are obviously improved, and the intestinal flora can be protected and repaired.
The above detailed description of the fermented beverage of traditional Chinese medicine with anti-tumor effect and the preparation method thereof with reference to the embodiments is illustrative and not restrictive, and several embodiments can be enumerated according to the limited scope, so that changes and modifications without departing from the general concept of the present invention shall fall within the protection scope of the present invention.

Claims (6)

1. A traditional Chinese medicine fermented beverage with anti-tumor effect is characterized in that effective components comprise medlar and honeysuckle co-extract, sugar solution, lactic acid bacteria, yeast and acetic acid bacteria, wherein each 500mL of medlar and honeysuckle co-extract contains 300mL of sugar solution and 1 × 10 mL of lactic acid bacteria3-1×1013CFU, Yeast 1 × 103-1×1013CFU, Acetobacter xylinum 1 × 103-1×1013CFU。
2. The fermented beverage of traditional Chinese medicine with anti-tumor efficacy according to claim 1, characterized in that: the medlar and honeysuckle co-extract is prepared by the following method: mixing 10-80g of dried fructus Lycii and 10-80g of flos Lonicerae, adding 500mL of purified water, extracting at 70-100 deg.C for 10-120min, filtering with 40 mesh sieve, extracting the residue with purified water at 70-100 deg.C for 10-120min, filtering with 40 mesh sieve, and mixing the two filtrates together.
3. The fermented beverage of traditional Chinese medicine with anti-tumor efficacy according to claim 1, characterized in that: the sugar solution is prepared by the following method: mixing 10-100g of white granulated sugar and 10-100g of honey, adding 300mL of purified water, dissolving, and filtering to obtain the final product.
4. The traditional Chinese medicine fermented beverage with anti-tumor effect according to claim 1, wherein each 500mL of the medlar and honeysuckle co-extract contains 300mL of sugar solution and 1 × 10 mL of lactic acid bacteria11CFU, Yeast 1 × 109CFU, Acetobacter xylinum 1 × 1010CFU。
5. The fermented beverage of traditional Chinese medicine with anti-tumor efficacy according to claim 1, characterized in that: the mass ratio of the dry medlar to the honeysuckle is 7: 3.
6. The method for preparing fermented beverage of Chinese traditional medicine with anti-tumor effect as claimed in claim 1, is characterized in that: the method comprises the following specific steps:
(1) mixing the extracts of fructus Lycii and flos Lonicerae with sugar solution, adding purified water, balancing to 1L, sterilizing at 121 deg.C for 20min, cooling to room temperature, inoculating lactobacillus, yeast and acetic acid bacteria, fermenting at 20-40 deg.C for 3-15 d;
(2) the fermentation broth was centrifuged under sterile conditions: centrifuging at 10000rpm for 5-20min at 2000-.
CN201710319498.1A 2017-05-09 2017-05-09 A kind of herb fermenting beverage with antitumor efficacy and preparation method thereof Pending CN107136370A (en)

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Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107496593A (en) * 2017-09-14 2017-12-22 湖南晓林生物科技发展有限公司 A kind of plant extracts for treating ascites and preparation method thereof
CN108283709A (en) * 2018-04-26 2018-07-17 阮忠宁 A kind of Chinese medicine composition with enhancing immunity of organisms function
CN110754553A (en) * 2019-12-04 2020-02-07 哈尔滨商业大学 Probiotic fermented medicinal and edible Chinese herbal tea beverage for preventing cancer and preparation method thereof
CN111973647A (en) * 2019-05-21 2020-11-24 大江生医股份有限公司 Preparation method of honeysuckle flower fermentation product and application of honeysuckle flower fermentation product in improving skin appearance and resisting aging

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107496593A (en) * 2017-09-14 2017-12-22 湖南晓林生物科技发展有限公司 A kind of plant extracts for treating ascites and preparation method thereof
CN108283709A (en) * 2018-04-26 2018-07-17 阮忠宁 A kind of Chinese medicine composition with enhancing immunity of organisms function
CN111973647A (en) * 2019-05-21 2020-11-24 大江生医股份有限公司 Preparation method of honeysuckle flower fermentation product and application of honeysuckle flower fermentation product in improving skin appearance and resisting aging
CN111973647B (en) * 2019-05-21 2023-05-12 大江生医股份有限公司 Preparation method of honeysuckle fermentation product and application of honeysuckle fermentation product in improving skin appearance and resisting aging
CN110754553A (en) * 2019-12-04 2020-02-07 哈尔滨商业大学 Probiotic fermented medicinal and edible Chinese herbal tea beverage for preventing cancer and preparation method thereof

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