KR101374696B1 - Staphylococcus pasteuri RSP-1 and its use - Google Patents

Abstract

The present invention is Staphylococcus pasteuri RSP-1 (KACC 91660P), a novel strain having excellent growth inhibitory activity against harmful bacteria such as food poisoning bacteria, particularly antibiotic resistant Staphylococcus aureus, and these strains, cultures or cultures thereof, or It relates to a method for inhibiting harmful bacteria, such as food poisoning bacteria, characterized in that using the antagonist separated from the culture or culture thereof.

Description

Staphylococcus pastry RSP-1 and its use {Staphylococcus pasteuri RSP-1 and its use}

The present invention relates to a novel strain for inhibiting the growth of harmful bacteria and its use, and in particular to the growth of food poisoning bacteria in agricultural products and the like using novel strains having growth inhibitory activity against food poisoning bacteria, such as antibiotic resistant Staphylococcus aureus. It is about a method to suppress.

Staphylococcus aureus, the world's most problematic pathogen, is a Gram-positive bacterium that is detected in about 30% of the entire population, and is widely known as a pathogen causing infection in hospitals. The harmful bacteria primarily cause skin and soft tissues to cause pyoderma, and may cause life-threatening serious systemic infections such as osteomyelitis, endocarditis, sepsis and bacteremia. Staphylococcal antibacterial agents, such as penicillin and methicillin, have been used as the main therapeutic agents for infectious diseases for a long time because of their excellent effects. Antibiotic-resistant Staphylococcus aureus continues to increase worldwide.

In Korea, the emergence of resistant strains due to indiscriminate misuse of antibiotics is particularly problematic. For example, methicillin resistance rate is very high, around 50%, and according to a recent study by the Korean Institute for Infectious Disease Control, about 75% of MRSA strains are isolated from most university hospitals and general hospitals.

Meanwhile, in addition to Staphylococcus aureus, Listeria monocytogenes and Bacillus cereus may cause food poisoning. Food-poisoning causes symptoms such as vomiting and diarrhea and changes in the intestinal flora caused by oral intestinal infection of pathogens. In particular, Listeria monocytogenes pollutes most foods such as meat, shellfish, cheese, ice cream, frozen foods, and is a deadly pathogen that causes miscarriage, sepsis, meningitis, and food poisoning in pregnant women, newborns, and senior citizens. When Listeria monocytogenes is subjected to osmotic stress such as high salinity, high sugar content and dryness, the osmolyte, which is an osmolyte, accumulates in the cell, thereby maintaining intracellular swelling and proliferating at low temperature (4 ° C). As it can survive in the heat treatment process, attention has been focused on food hygiene.

On the other hand, chemical preservatives have been used commercially as a preservative to inhibit the growth of food poisoning microorganisms, but the recent phenomenon of avoiding synthetic preservatives has been prominent due to the increase of health-oriented needs and stability of consumers. In order to cope with these problems, attention is focused on the development of a natural antimicrobial active material harmless to the human body. In particular, there is a great need to effectively suppress antibiotic resistant harmful bacteria.

1. Korean Unexamined Patent Publication No. 10-2008-0083840 2. Korean Registered Patent Publication No. 10-0715730

Therefore, the technical problem to be achieved by the present invention is to inhibit the growth of harmful bacteria, in particular food poisoning bacteria, and furthermore antibiotic-resistant harmful bacteria in an effective and environmentally friendly way, and using these strains to suppress the growth of harmful bacteria in agricultural products, food, etc. To provide a way.

In order to achieve the above technical problem, the present invention provides a novel strain Staphyloccus pasteuri RSP-1 (KACC 91660P), which effectively suppresses the growth of harmful bacteria such as food poisoning bacteria, especially antibiotic resistant Staphylococcus aureus do.

The present inventors have selected Staphylococcus pasteuri RSP-1 which has an antagonistic resistance against antibiotic resistant Staphylococcus aureus among 400 Staphylococcus bacteria isolated from agricultural products, and there is no bacteriocin producing gene previously reported in the selected Staphylococcus pasteuri RSP-1. It was confirmed using a PCR (polymerase chain reaction).

In addition, the selected Staphylococcus pasteuri RSP-1 exhibited high antagonism against Gram-positive food poisoning bacteria such as Listeria monocytogenes and Bacillus cereus as well as Staphylococcus aureus.

Staphylococcus pasteuri RSP-1 strain according to the present invention can be cultured in large quantities by a conventional method for culturing Staphylococcus microorganisms. As the culture medium, a medium composed of carbon source, nitrogen source, vitamins and minerals can be used. For example, NB (nutrient broth), TSB (tryptic soy broth) liquid medium, or TSA (tryptic soy agar) solid medium can be used. Incubation can be carried out under conventional culture conditions, for example at about 37 ° C. for about 10-72 hours. Centrifugation or filtration may be performed to remove the culture medium in the culture and recover only concentrated cells, and this step may be performed according to the needs of those skilled in the art. The concentrated microbial cells can be preserved by freezing or freeze-drying according to a conventional method so as not to lose their activity.

In addition, the microorganism according to the present invention may be improved or improved to have equivalent activity or better activity by conventional physicochemical mutation methods and the like known in the art.

The invention also provides an antagonist isolated from the culture or culture of Staphylococcus pasteuri RSP-1 (KACC 91660P).

The antagonist of the present invention has a thermal stability and pH safety as a protein and maintains its antagonism even when diluted several hundred times, it can be usefully used as a composition for inhibiting harmful bacteria growth.

The antagonist may be separated by a conventional method known in the art, but preferably, after culturing Staphylococcus pasteuri RSP-1 (KACC 91660P) to recover the culture supernatant; Adding hydrophobic resin to the culture supernatant and adsorbing at 20-25 ° C. for 6-10 hours; Removing the remaining culture supernatant without adsorption, washing and diluting with 70% 2-propanol containing 0.1% trifluorothioacetate (TFA); And distilling the diluting solution and suspending it in Tris-Cl buffer at pH 6-9 to separate the antagonist.

Or, preferably, culturing Staphylococcus pasteuri RSP-1 (KACC 91660P) and then centrifuging to recover the culture supernatant; The ammonium sulfate precipitate formed by adding 10-40% ammonium sulfate to the culture supernatant was removed by centrifugation, and the ammonium sulfate precipitate formed by adding 50-70% ammonium sulfate was removed by centrifugation, and 80 Adding -90% ammonium sulfate to obtain the resulting ammonium sulfate precipitate; Suspending the obtained ammonium sulphate precipitate in Tris-Cl buffer at pH 6-9 and heat treatment to remove the resulting precipitate; And separating the antagonist by removing the precipitate and permeating the remaining supernatant to a membrane having a pore size of 50 kDa.

The invention also provides a probiotic with probiotic activity comprising Staphylococcus pasteuri RSP-1 (KACC 91660P), its culture or culture, or an antagonist isolated from its culture or culture do.

In addition, the present invention Staphylococcus pasteuri ( Staphylococcus pasteuri ) RSP-1 (KACC 91660P), a culture or culture thereof, or a composition for inhibiting harmful bacteria comprising an antagonist separated from the culture or culture thereof To provide.

The probiotic or composition may contain the antagonist isolated from the crushed cell wall fraction of the strain according to the present invention, live bacteria, dead bacteria, dry bacteria, or cultures or cultures thereof, or cultures or cultures thereof, Suitable excipients or carriers may further be included. The culture medium is culture medium itself in which the strain according to the invention cultured in a suitable liquid medium, the filtrate (filtered or centrifuged supernatant) from which the strain is removed by filtration or centrifugation, the culture solution is sonicated or dissolved in the culture solution Cell lysates obtained by treating enzymes (lysozyme), but are not limited thereto. In addition, the probiotic or composition may contain a culture medium such as a solid medium culture, or a dried product or extract of the culture solution, but is not limited thereto.

Staphylococcus pasteuri RSP-1 of the present invention, or harmful bacteria that can be used as a culture or culture thereof may be Staphylococcus aureus , Staphylococcus xylosus, Staphylococcus xylosus, Bacillus cereus ), Clavibacter michiganensis , Listeria monocytogenes , Xanthomonas albilineans , and the like, and in particular, Staphylococcus pasteuri RSP-1 of the present invention is Staphylococcus aurea . It is very useful for suppressing food poisoning bacteria such as Staphylococcus aureus , Bacillus cereus and Listeria monocytogenes .

Staphylococcus pasteuri ( Staphylococcus pasteuri ) according to the invention RSP-1 (KACC 91660P), a culture or culture thereof, or a composition for inhibiting harmful bacteria comprising an antagonist separated from the culture or culture thereof It can be used as food, feed additives, detergent additives and the like.

Accordingly, the present invention is characterized in that it comprises a Staphylococcus pasteuri RSP-1 (KACC 91660P), a culture or culture thereof, or an antagonist isolated from the culture or culture thereof. Provide food for suppressing harmful bacteria.

There is no particular limitation on the kind of the food. Examples of Staphylococcus pasteuri RSP-1 (KACC 91660P), a culture or a culture thereof, or a food to which an antagonist separated therefrom are added according to the present invention, such as meat, sausage, bread, Chocolate, candy, snacks, confectionery, pizza, ramen, other noodles, gum, dairy products, including ice cream, various soups, beverages, tea, drinks, alcoholic beverages and vitamin complexes, food in the ordinary sense, In particular, it includes all health food. The health food containing the composition according to the present invention is for the purpose of inhibiting the growth of harmful bacteria such as tea, jelly, juice, extract, beverages, etc., wherein the strain, its culture or culture, or the antagonist isolated therefrom as an active ingredient. Folk remedies. When the composition according to the present invention is used as a food additive, each of the above may be added as it is or used with other food or food ingredients, and may be appropriately used according to a conventional method. The food of the present invention may contain various flavors, sweeteners or natural carbohydrates, etc. as additional ingredients, like conventional foods. Natural carbohydrates described above include glucose, monosaccharides such as fructose, maltose, disaccharides such as sucrose, and polysaccharides such as dextrin, cyclodextrin, sugar alcohols such as xylitol, sorbitol, and erythritol. As the sweetening agent, natural sweetening agents such as tautin and stevia extract, synthetic sweetening agents such as saccharin, aspartame, and the like can be used. Foods of the present invention other than the above include various nutrients, vitamins, electrolytes, flavors, coloring agents, pectic acid and salts thereof, alginic acid and salts thereof, organic acids, protective colloidal thickeners, pH adjusting agents, stabilizers, preservatives, glycerin, alcohols, And a carbonation agent used for the carbonated beverage. In addition, the food containing the composition according to the present invention may contain flesh for preparing natural fruit juice, fruit juice beverage and vegetable beverage. These components may be used independently or in combination. Such harmful bacterium growth inhibiting food of the present invention is excellent in suppressing the growth of harmful bacteria without adverse effects on the human body, and is easy to take and can be stored for a long time. The amount of the active ingredient to be mixed can be suitably determined according to the intended use (prevention, health or therapeutic treatment).

The present invention also includes Staphylococcus pasteuri RSP-1 (KACC 91660P), a culture or culture thereof, or an antagonist for inhibiting harmful bacteria, characterized in that it comprises an antagonist isolated from the culture or culture thereof. Or it provides a feed composition comprising the same.

Feed additives of the present invention can be added to plant or animal feed. Feed additives of the present invention may be 20 to 90% high concentrate or in the form of powder or granules. The feed additives include organic acids such as citric acid, fumaric acid, adipic acid, lactic acid and malic acid, phosphates such as sodium phosphate, potassium phosphate, acid pyrophosphate and polyphosphate (polyphosphate), polyphenols, catechins (catechin) and lees extracts ( rosemary extract), vitamin C, green tea extract, licorice extract, chitosan, tannic acid, phytic acid and may further include any one or more of natural antioxidants. The feed additive of the present invention and the feed containing the same are supplementary ingredients such as amino acids, inorganic salts, vitamins, antibiotics, antimicrobials, antioxidants, antifungal enzymes, live microbial agents, etc. Wheat, oats, barley, corn and rice; Vegetable protein feedstuffs, for example, based on rapeseed, soybeans and sunflower; Animal protein feeds such as blood, meat, bone meal and fish meal; It is preferable to mix all of the dry ingredients comprising the sugar and the milk product such as the various powdered milk and the whey powder and the dry additive and then mix the liquid ingredient with the ingredient to be liquid after heating, In addition to the main components such as fat and vegetable fat, can be used together with materials such as nutritional supplements, digestion and absorption enhancers, growth promoters, disease prevention agents and the like. The feed additive may be administered alone in combination with other feed additives in an edible carrier. In addition, the feed additives can be easily administered as a top dressing or directly mixed into the feed or separately from the feed, in a separate oral formulation, by injection or transdermal or in combination with other ingredients. Typically, a single daily dose or divided daily doses can be used, as is well known in the art. When the feed additive is administered separately from the feed, the dosage form of the culture extract, as is well known in the art, can be prepared in an immediate release or sustained release formulation in combination with a non-toxic pharmaceutically acceptable edible carrier. Such edible carriers may be solid or liquid, for example corn starch, lactose, sucrose, soy flakes, peanut oil, olive oil, sesame oil and propylene glycol. If a solid carrier is used, the dosage form may be a tablet, a capsule, a powder, a torokee or a sugar-containing tablet or a top dressing in an undispersible form. If a liquid carrier is used, it may be in the form of soft gelatin capsules or syrups or liquid suspensions, emulsions or solutions. In addition, the dosage form may contain auxiliaries such as preservatives, stabilizers, wetting or emulsifying agents, solution promoters and the like. In addition, the animal feed comprising the feed additive of the present invention may be any protein-containing organic wheat flour conventionally used to meet the dietary needs of the animal. Such protein-containing flours typically consist mainly of corn, soy flour, or corn / bean flour mixtures. The feed additive may be used by adding to the feed by dipping, spraying or mixing. The invention is applicable to a number of animal diets, including mammals, poultry and fish. More specifically, the diet is commercially important mammals such as pigs, cows, sheep, goats, laboratory rodents (rats, mice, hamsters and gerbils), furry animals (eg, mink and fox), and Zoo animals (eg monkeys and tailless monkeys), as well as pets (eg cats and dogs). Commercially important poultry typically include chickens, turkeys, ducks, geese, pheasants and quails. Commercially raised fish, such as trout, may also be included. Also, after the feed mixture is thoroughly mixed, lightly viscous granulation or granulation material is obtained depending on the degree of crushing of the components. It is fed as a mash or formed into the desired discrete shape for further processing and packaging. At this time, in order to prevent separation during storage, it is preferable to add water to the feed and then go through the usual pelletization, expansion, or extrusion process. Excess water can be dried off.

The present invention also includes Staphylococcus pasteuri RSP-1 (KACC 91660P), a culture medium or culture thereof, or a detergent additive for inhibiting harmful bacteria, wherein the antagonist is separated from the culture medium or culture thereof. Or it provides a detergent composition comprising the same.

The detergent composition according to the invention may comprise one or more surfactants. The surfactant may be anionic, non-ionic, cationic, amphoteric or zwitter ionic type, or mixtures thereof. Representative examples of the anionic surfactants include linear alkylbenzene sulfonates (LAS), alkyl sulfates (AS), alpha olefin sulfonates (AOS), alcohol ethoxy sulfates (AES) and alkali metal salts of natural fatty acids. Examples of non-ionic surfactants include alkyl polyethylene glycol ethers, nonylphenol polyethylene glycol ethers, fatty acid esters of sucrose and glucose, and esters of polyethoxylated alkylglucosides. Detergent compositions of the present invention may also contain abrasives, bleaches, surfactants, corrosion inhibitors, metallurgical agents, stain-repositioning agents, perfumes, enzymes and bleach stabilizers, formulation aids, optical brighteners, foam boosters, chelating agents, fillers, And other detergent ingredients known in the art, such as fabric softeners and the like. Detergent compositions of the present invention may be formulated in any convenient form, such as powders, solutions.

The invention also relates to Staphylococcus pasteuri ) RSP-1 (KACC 91660P), a culture or culture thereof, or a antagonist isolated from the culture or culture thereof provides a method for inhibiting harmful bacteria.

The present invention is characterized by using Staphylococcus pasteuri RSP-1, a novel strain having excellent growth inhibitory activity against harmful bacteria such as food poisoning bacteria, particularly antibiotic resistant Staphylococcus aureus, and such strains, cultures or cultures thereof, or antagonists isolated therefrom. It provides a method for suppressing harmful bacteria such as food poisoning bacteria. By using a specific strain according to the present invention it is possible to reduce the loss of crops due to contamination of the production and distribution of agricultural products, it is possible to effectively control antibiotic resistant microorganisms.

1 is staphylococcus Shown is the separation and concentration of antagonists from pasteuri RSP-1.
Figure 2 shows the results of evaluation of the harmful bacteria growth inhibitory activity of the antagonist.
Figure 3 shows the results of the thermal stability test of the antagonist.

Hereinafter, embodiments of the present invention will be described in detail to facilitate understanding of the present invention. However, the embodiments according to the present invention can be modified into various other forms, and the scope of the present invention should not be construed as being limited to the following embodiments. Embodiments of the invention are provided to more fully describe the present invention to those skilled in the art.

Example 1 Staphylococcus pasteuri  Screening of RSP-1 Strains

Among 400 Staphylococcus isolates from agricultural products, optimal strains with antagonistic activity against antibiotic resistant Staphylococcus were selected.

Wrapping vegetables, bud vegetables, fresh produce was not only the food, separated 394 Staphylococci strains, etc. animals, people, the major food poisoning bacteria of which (for example, Staphylococcus aureus) 346 strain by the navigation bacteriocin producing strains intended for bacteriocins 139 Staphylococci strains were produced, and 40 strains of Staphylococci were selected by evaluating the inhibitory activity against various harmful food poisoning bacteria, and one of the 40 strains showed the highest activity. The strains were identified as Staphylococcus pasteuri through molecular systemic analysis and other biochemical analysis based on the 16s rDNA sequence described below. The strain was named Staphylococcus pasteuri RSP-1, and as of October 4, 2011. Korean Agricultural Culture Collection (KACC) And was assigned accession number KACC 91660P.

Example 2 Selected Strains Staphylococcus pasteuri  Identification, fungal properties and culture conditions of RSP-1

Morphological analysis

Selected strains of the invention were incubated in TSA plate medium and the morphology of the colonies was observed under a microscope. Colony forms of the selected strains are shown in Table 1 below.

Sugar usability analysis

The sugar availability of the strain according to the present invention was investigated. Sugar fermentation characteristics were investigated in accordance with the experimental method of the supplier using the API StaphID kit (Bio Merioux), the results are shown in Table 2 below.

16S rDNA  analysis

16S rDNA sequencing was performed to identify the selected strains and the results are shown in SEQ ID NO: 1. The RSP-1 selected strains of this invention was identified as a strain isolated showed 16S rDNA homology with Staphylococcus pasteuri 99% Staphylococcus pasteuri.

Culture Characteristics of Selected Strains

Selected strains of the present invention were incubated at about 37 ° C. for about 10-72 hours in tryptic soy agar (TSA).

[Example 3] Staphylococcus pasteuri  Evaluation of food poisoning inhibitory effect of RSP-1 strain

Staphylococcus Primary Growth Inhibitory Activity of the Genus Strains

In order to investigate whether Staphylococcus pasteuri RSP-1 according to the present invention has a growth inhibitory activity against antibiotic resistant Staphylococcus aureus, the effects of antibiotic resistant Staphylococcus xylosus, Staphylococcus aureus and Staphylococcus pasteuri on the growth of other strains were analyzed. The antibiotic resistant Staphylococcus aureus strains were distributed from antibiotic resistant strain banks , and Staphylococcus xylosus, and Staphylococcus pasteuri strains were isolated and identified directly in the laboratory.

Specifically, the indicators for measuring antimicrobial activity were inoculated in TSA medium (Tryptic soy liquid medium), respectively, and incubated overnight at 37 ° C. 1% of each indicator culture medium was inoculated into soft agar medium (0.8% agar) cooled to 45 ° C. The soft agar medium inoculated with the indicator bacteria was poured into a disposable medium dish and cooled to prepare a plate medium. A paper disk was placed on the prepared flat medium, and the strain culture solution of the present invention prepared on the paper disk was instilled by 10ul, and then incubated at an optimal growth temperature of each indicator for 24 hours. After incubation, the antimicrobial activity of each strain was measured by measuring the diameter of the growth zone (Clear zone) for each indicator. By comparing the diameters of growth inhibition rings, strains with high antimicrobial activity were selected for each strain. Depending on the diameter size of the low-ring ring showing the antimicrobial activity of the selected strains-(not inhibiting growth), + (lower ring diameter less than about 8mm), ++ (low ring diameter is about 8-10mm) and ++ It evaluated as + (lower ring diameter about 10 mm or more).

The results are shown in Tables 3 to 5 below.

As shown in Tables 3 and 5, Staphylococcus pasteuri RSP-1, a new strain according to the present invention, showed growth inhibitory activity in various strains belonging to Staphylococcus aureus. In addition, as shown in Table 4, it showed growth inhibitory activity for some other strains belonging to the same Staphylococcus pasteuri .

Staphylococcus Analysis of Secondary Growth Inhibition Activity in Other Strains

Selected Staphylococcus Inhibition of bacterial growth of pasteuri RSP-1 by Listeria as well as Staphylococcus aureus monocytogenes , Bacillus Other antibiotic resistant Gram-positive food poisoning bacteria such as cereus were also identified. The antibiotic resistant Gram-positive food poisoning bacteria were all distributed from an antibiotic resistant strain bank. Experimental method was the same as the primary growth inhibitory activity assay. The results are shown in Table 6 below.

As shown in Table 6 above, selected Staphylococcus pasteuri RSP-1 is a Listeria as well as Staphylococcus aureus monocytogenes , Clavibacter michiganensis , Xanthomonas albilineans , and Bacillus cereus KCTC1092 also showed growth inhibition activity.

[ Example  4] Staphylococcus pasteuri RSP -1 Bacteriocin Study

First, it was confirmed by PCR whether the selected Staphylococcus pasteuri RSP-1 has a previously reported bacteriocin producing gene. Table 7 shows various Staphylococcus PCR primer sequences of Staphylococcin-producing genes known to be present in the genus.

Check result Staphylococcus of the present invention pasteuri RSP-1 did not have a previously reported bacteriocin-producing gene, and cloned and sequenced the nukacin-producing gene, Staphylococcus The bacteriocin-producing gene of pasteuri RSP-1 was confirmed to have a different sequence from the nukacin-producing gene.

[Example 5] Staphylococcus pasteuri  Separation and Concentration of Antagonists from RSP-1

Method using interaction with hydrophobic resin

20 g of XAD-16 resin was added to 400 ml of the supernatant, and the interaction was induced at 23 ° C. for 6 hours. Then, the supernatant was removed, washed with 100 ml of distilled water, and then again with 100 ml of 40% ethanol. It was then diluted with 400 ml of 70% 2-propanol containing 0.1% TFA, then distilled under vacuum at 40 ° C. and suspended in 20 mM Tris-Cl buffer at pH 8.0 to isolate and concentrate only the antagonist (FIG. 1 left).

Method using Ammonium Sulfate Precipitation

30% precipitate of ammonium sulfate was removed by centrifugation and precipitated up to 60%, then 60% precipitate was removed by centrifugation, and the supernatant was again precipitated to 80%, and the precipitate was suspended in 20 mM Tris-Cl buffer at pH 8.0. Heat-treated at 100 ° C. for 30 minutes. Through heat treatment, a large amount of protein was denatured and precipitated. The precipitate was removed by centrifugation, and finally, the remaining supernatant was separated and concentrated to 50 kDa pore size memebrane (right side of FIG. 1).

[Example 6] Staphylococcus pasteuri  Evaluation of Hazardous Bacteria Growth Inhibitory Activity of Concentrated Antagonists from RSP-1

The antagonists obtained through the method of Example 5 were diluted 2 times, 4 times, 8 times, 16 times, 32 times, 64 times and 128 times to test their antagonism. As a result, the antagonist was confirmed even when diluting 128 times as well as diluting 128 times (FIG. 2).

[Example 7] Staphylococcus pasteuri  Characterization of Antagonists Concentrated and Separated from RSP-1

Thermal safety of antagonists

The antagonists obtained through the method of Example 5 were heat treated at 80, 90, and 100 ° C. for 10, 20, and 30 minutes, respectively, and then tested for activity. As a result, even after the heat treatment for 30 minutes at 100 ℃ it was very safe there was no change in the antagonism, it was confirmed that the thermal stability in all cases (Fig. 3).

Confirmation of pH Safety of Antagonists

As a result of confirming the pH safety of the antagonist obtained through the method of Example 5, it showed activity in a relatively wide range up to pH 4-10, particularly high pH between 7-8.

Protease Sensitivity of Antagonists

After treating the antagonist obtained by the method of Example 5 with various proteases, the activity of the protease was removed by heat treatment at 70 ° C. for 10 minutes, and then the antagonist of the antagonist was confirmed. As a result, it was confirmed that the antagonist is a proteinaceous substance through the phenomenon that the activity disappears after the treatment of the protease.

National Institute of Agricultural Science KACC91660P 20111004

Claims (13)

Staphylococcus pasteuri RSP-1 (KACC 91660P). Staphylococcus pasteuri RSP-1 (KACC 91660P), a probiotic having probiotic activity, including a culture or a culture thereof. Staphylococcus pasteuri RSP-1 (KACC 91660P), a composition for inhibiting harmful bacteria comprising a culture medium or a culture thereof,
The harmful bacterium is Staphylococcus aureus , Bacillus cereus , or food poisoning bacteria, including Listeria monocytogenes , Staphylococcus xylosus , Clavibacter madga Clavibacter michiganensis and Xanthomonas albilineans ( Xanthomonas albilineans ) characterized in that at least one harmful bacteria selected from the group consisting of. delete delete Staphylococcus pasteuri (Sphyphylococcus pasteuri ) RSP-1 (KACC 91660P), a method for inhibiting harmful bacteria, characterized in that the treatment with a culture or a culture thereof,
The harmful bacterium is Staphylococcus aureus , Bacillus cereus , or food poisoning bacteria, including Listeria monocytogenes , Staphylococcus xylosus , Clavibacter madga Clavibacter michiganensis and Xanthomonas albilineans ( Xanthomonas albilineans ) characterized in that at least one harmful bacteria selected from the group consisting of.
delete delete delete delete delete delete delete

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