CN116477772B - Method for removing chromium and desulfurizing bacillus cereus in water and application thereof - Google Patents
Method for removing chromium and desulfurizing bacillus cereus in water and application thereof Download PDFInfo
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- CN116477772B CN116477772B CN202310533207.4A CN202310533207A CN116477772B CN 116477772 B CN116477772 B CN 116477772B CN 202310533207 A CN202310533207 A CN 202310533207A CN 116477772 B CN116477772 B CN 116477772B
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- 239000011651 chromium Substances 0.000 title claims abstract description 79
- VYZAMTAEIAYCRO-UHFFFAOYSA-N Chromium Chemical compound [Cr] VYZAMTAEIAYCRO-UHFFFAOYSA-N 0.000 title claims abstract description 74
- 229910052804 chromium Inorganic materials 0.000 title claims abstract description 74
- 241000193755 Bacillus cereus Species 0.000 title claims abstract description 50
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 title claims abstract description 26
- 238000000034 method Methods 0.000 title claims abstract description 24
- 230000003009 desulfurizing effect Effects 0.000 title claims abstract description 10
- 239000011593 sulfur Substances 0.000 claims abstract description 78
- 229910052717 sulfur Inorganic materials 0.000 claims abstract description 78
- NINIDFKCEFEMDL-UHFFFAOYSA-N Sulfur Chemical compound [S] NINIDFKCEFEMDL-UHFFFAOYSA-N 0.000 claims abstract description 76
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims abstract description 38
- 229910052757 nitrogen Inorganic materials 0.000 claims abstract description 17
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims abstract description 13
- 239000001963 growth medium Substances 0.000 claims abstract description 13
- 229910052760 oxygen Inorganic materials 0.000 claims abstract description 13
- 239000001301 oxygen Substances 0.000 claims abstract description 13
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims abstract description 8
- 229910052799 carbon Inorganic materials 0.000 claims abstract description 8
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 claims abstract description 6
- 229910052698 phosphorus Inorganic materials 0.000 claims abstract description 6
- 239000011574 phosphorus Substances 0.000 claims abstract description 6
- 238000011081 inoculation Methods 0.000 claims abstract description 5
- 238000004321 preservation Methods 0.000 claims abstract description 4
- -1 sulfur ion Chemical class 0.000 claims description 10
- 229910001430 chromium ion Inorganic materials 0.000 claims description 9
- 238000012258 culturing Methods 0.000 claims description 8
- 239000002609 medium Substances 0.000 claims description 6
- ZCDOYSPFYFSLEW-UHFFFAOYSA-N chromate(2-) Chemical compound [O-][Cr]([O-])(=O)=O ZCDOYSPFYFSLEW-UHFFFAOYSA-N 0.000 claims description 3
- SOCTUWSJJQCPFX-UHFFFAOYSA-N dichromate(2-) Chemical compound [O-][Cr](=O)(=O)O[Cr]([O-])(=O)=O SOCTUWSJJQCPFX-UHFFFAOYSA-N 0.000 claims description 3
- 238000006477 desulfuration reaction Methods 0.000 abstract description 14
- 230000023556 desulfurization Effects 0.000 abstract description 14
- 238000005516 engineering process Methods 0.000 abstract description 6
- 238000012216 screening Methods 0.000 abstract description 2
- 239000010865 sewage Substances 0.000 abstract 1
- 241000894006 Bacteria Species 0.000 description 56
- 230000015556 catabolic process Effects 0.000 description 34
- 238000006731 degradation reaction Methods 0.000 description 34
- 238000000855 fermentation Methods 0.000 description 25
- 230000004151 fermentation Effects 0.000 description 25
- PXHVJJICTQNCMI-UHFFFAOYSA-N Nickel Chemical compound [Ni] PXHVJJICTQNCMI-UHFFFAOYSA-N 0.000 description 16
- 239000007788 liquid Substances 0.000 description 16
- 239000011734 sodium Substances 0.000 description 12
- 230000001580 bacterial effect Effects 0.000 description 10
- 239000000243 solution Substances 0.000 description 9
- QAOWNCQODCNURD-UHFFFAOYSA-L sulfate group Chemical group S(=O)(=O)([O-])[O-] QAOWNCQODCNURD-UHFFFAOYSA-L 0.000 description 9
- 229910052759 nickel Inorganic materials 0.000 description 8
- 230000000694 effects Effects 0.000 description 7
- 239000003795 chemical substances by application Substances 0.000 description 6
- 238000011160 research Methods 0.000 description 6
- 238000006243 chemical reaction Methods 0.000 description 5
- 229910001385 heavy metal Inorganic materials 0.000 description 5
- 238000007747 plating Methods 0.000 description 5
- 229910052782 aluminium Inorganic materials 0.000 description 4
- XAGFODPZIPBFFR-UHFFFAOYSA-N aluminium Chemical compound [Al] XAGFODPZIPBFFR-UHFFFAOYSA-N 0.000 description 4
- 239000008367 deionised water Substances 0.000 description 4
- 229910021641 deionized water Inorganic materials 0.000 description 4
- 229910001873 dinitrogen Inorganic materials 0.000 description 4
- 244000005700 microbiome Species 0.000 description 4
- 238000002360 preparation method Methods 0.000 description 4
- 239000000126 substance Substances 0.000 description 4
- RWSOTUBLDIXVET-UHFFFAOYSA-N Dihydrogen sulfide Chemical compound S RWSOTUBLDIXVET-UHFFFAOYSA-N 0.000 description 3
- AZDRQVAHHNSJOQ-UHFFFAOYSA-N alumane Chemical group [AlH3] AZDRQVAHHNSJOQ-UHFFFAOYSA-N 0.000 description 3
- 238000011161 development Methods 0.000 description 3
- 238000009713 electroplating Methods 0.000 description 3
- 229910000037 hydrogen sulfide Inorganic materials 0.000 description 3
- 230000003993 interaction Effects 0.000 description 3
- 238000002386 leaching Methods 0.000 description 3
- 239000010802 sludge Substances 0.000 description 3
- 239000002351 wastewater Substances 0.000 description 3
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 description 2
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical group [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 description 2
- NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 description 2
- QAOWNCQODCNURD-UHFFFAOYSA-N Sulfuric acid Chemical compound OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 description 2
- 238000002835 absorbance Methods 0.000 description 2
- 230000004913 activation Effects 0.000 description 2
- 238000010170 biological method Methods 0.000 description 2
- 230000000593 degrading effect Effects 0.000 description 2
- RWSXRVCMGQZWBV-WDSKDSINSA-N glutathione Chemical compound OC(=O)[C@@H](N)CCC(=O)N[C@@H](CS)C(=O)NCC(O)=O RWSXRVCMGQZWBV-WDSKDSINSA-N 0.000 description 2
- 239000002054 inoculum Substances 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- VNWKTOKETHGBQD-UHFFFAOYSA-N methane Chemical compound C VNWKTOKETHGBQD-UHFFFAOYSA-N 0.000 description 2
- 238000002161 passivation Methods 0.000 description 2
- 238000000746 purification Methods 0.000 description 2
- 239000007787 solid Substances 0.000 description 2
- 150000003568 thioethers Chemical class 0.000 description 2
- XXCLTDOQUREFHZ-UHFFFAOYSA-L C(C)(=O)[O-].[Na+].C(C)(=O)[O-].[Zn+2] Chemical compound C(C)(=O)[O-].[Na+].C(C)(=O)[O-].[Zn+2] XXCLTDOQUREFHZ-UHFFFAOYSA-L 0.000 description 1
- UGFAIRIUMAVXCW-UHFFFAOYSA-N Carbon monoxide Chemical compound [O+]#[C-] UGFAIRIUMAVXCW-UHFFFAOYSA-N 0.000 description 1
- 208000005623 Carcinogenesis Diseases 0.000 description 1
- KRKNYBCHXYNGOX-UHFFFAOYSA-K Citrate Chemical compound [O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O KRKNYBCHXYNGOX-UHFFFAOYSA-K 0.000 description 1
- 108010024636 Glutathione Proteins 0.000 description 1
- BZORFPDSXLZWJF-UHFFFAOYSA-N N,N-dimethyl-1,4-phenylenediamine Chemical compound CN(C)C1=CC=C(N)C=C1 BZORFPDSXLZWJF-UHFFFAOYSA-N 0.000 description 1
- UCKMPCXJQFINFW-UHFFFAOYSA-N Sulphide Chemical compound [S-2] UCKMPCXJQFINFW-UHFFFAOYSA-N 0.000 description 1
- 239000005864 Sulphur Substances 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 230000003213 activating effect Effects 0.000 description 1
- 229910000147 aluminium phosphate Inorganic materials 0.000 description 1
- XGGLLRJQCZROSE-UHFFFAOYSA-K ammonium iron(iii) sulfate Chemical compound [NH4+].[Fe+3].[O-]S([O-])(=O)=O.[O-]S([O-])(=O)=O XGGLLRJQCZROSE-UHFFFAOYSA-K 0.000 description 1
- 239000007864 aqueous solution Substances 0.000 description 1
- 229940072107 ascorbate Drugs 0.000 description 1
- 235000010323 ascorbic acid Nutrition 0.000 description 1
- 239000011668 ascorbic acid Substances 0.000 description 1
- 230000008953 bacterial degradation Effects 0.000 description 1
- 230000036952 cancer formation Effects 0.000 description 1
- 231100000504 carcinogenesis Toxicity 0.000 description 1
- 210000004027 cell Anatomy 0.000 description 1
- 210000000170 cell membrane Anatomy 0.000 description 1
- 238000001311 chemical methods and process Methods 0.000 description 1
- 239000012295 chemical reaction liquid Substances 0.000 description 1
- KRVSOGSZCMJSLX-UHFFFAOYSA-L chromic acid Substances O[Cr](O)(=O)=O KRVSOGSZCMJSLX-UHFFFAOYSA-L 0.000 description 1
- 150000001844 chromium Chemical class 0.000 description 1
- 229940001468 citrate Drugs 0.000 description 1
- 239000003245 coal Substances 0.000 description 1
- 230000000052 comparative effect Effects 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 229910052802 copper Inorganic materials 0.000 description 1
- 239000010949 copper Substances 0.000 description 1
- 238000007865 diluting Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 239000003344 environmental pollutant Substances 0.000 description 1
- 239000003337 fertilizer Substances 0.000 description 1
- 239000003546 flue gas Substances 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 229960003180 glutathione Drugs 0.000 description 1
- 239000010842 industrial wastewater Substances 0.000 description 1
- 230000003834 intracellular effect Effects 0.000 description 1
- 238000000691 measurement method Methods 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 239000013028 medium composition Substances 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 230000035772 mutation Effects 0.000 description 1
- 239000003345 natural gas Substances 0.000 description 1
- 125000001741 organic sulfur group Chemical group 0.000 description 1
- 230000003647 oxidation Effects 0.000 description 1
- 238000007254 oxidation reaction Methods 0.000 description 1
- 230000001590 oxidative effect Effects 0.000 description 1
- 239000000575 pesticide Substances 0.000 description 1
- 239000003208 petroleum Substances 0.000 description 1
- 238000005554 pickling Methods 0.000 description 1
- 238000005498 polishing Methods 0.000 description 1
- 238000010248 power generation Methods 0.000 description 1
- 239000010970 precious metal Substances 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 238000007670 refining Methods 0.000 description 1
- 238000001179 sorption measurement Methods 0.000 description 1
- RPBNQQGUJBCUGO-UHFFFAOYSA-N sulfanylidenechromium Chemical compound [S].[Cr] RPBNQQGUJBCUGO-UHFFFAOYSA-N 0.000 description 1
- 230000002194 synthesizing effect Effects 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 125000003396 thiol group Chemical group [H]S* 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/10—Inorganic compounds
- C02F2101/101—Sulfur compounds
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/10—Inorganic compounds
- C02F2101/20—Heavy metals or heavy metal compounds
- C02F2101/22—Chromium or chromium compounds, e.g. chromates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
- C12R2001/07—Bacillus
- C12R2001/085—Bacillus cereus
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/20—Air quality improvement or preservation, e.g. vehicle emission control or emission reduction by using catalytic converters
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- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Microbiology (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- Environmental & Geological Engineering (AREA)
- Tropical Medicine & Parasitology (AREA)
- Virology (AREA)
- Medicinal Chemistry (AREA)
- Biomedical Technology (AREA)
- Water Supply & Treatment (AREA)
- Hydrology & Water Resources (AREA)
- Biodiversity & Conservation Biology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Purification Treatments By Anaerobic Or Anaerobic And Aerobic Bacteria Or Animals (AREA)
Abstract
The invention discloses application of bacillus cereus in desulfurization, chromium removal and desulfurization in water and a method for desulfurizing, chromium removal and desulfurization in water by utilizing the bacillus cereus, belonging to the sewage treatment technology. The invention discloses a method for desulfurizing and removing chromium and desulfurizing in water by using bacillus cereus, which comprises the steps of using bacillus cereus Bacillus cereus FNXJ1-2-3 (with the preservation number of CGMCC No. 9683) obtained by screening and preserved, adding NaAC as carbon, and KNO 3 And NH 4 Cl is a nitrogen source, na 2 HPO 4 And KH 2 PO 4 Is a phosphorus source culture medium, is filled with nitrogen to remove oxygen and is then cultured, and the culture temperature, the culture time, the inoculation amount and the components of the culture medium are optimizedConcentration. The bacillus cereus disclosed by the invention can effectively remove sulfur and chromium in water.
Description
Technical Field
The invention belongs to the technical field of water treatment, and particularly relates to a method for removing chromium and sulfur from water by bacillus cereus and application thereof.
Background
In recent years, biological desulfurization technology is attracting attention as an environment-friendly and low-cost desulfurization technology, and compared with other chemical methods, the biological desulfurization technology has the advantages of mild reaction, simple equipment, low treatment cost, no secondary pollution and the like, is a desulfurization technology with great development prospect, and can be widely applied to natural gas desulfurization, biogas desulfurization, flue gas desulfurization and the like. There are two main categories of research directions for the treatment of sulfides by biological methods: one type of product is sulfate, and the other type of product is elemental sulfur. However, the sulfate is easily reduced by sulfate reducing bacteria to generate hydrogen sulfide, and the secondary pollution is caused by the sulfate reducing bacteria, so that the sulfate is treated in other modes, the sulfate is converted into elemental sulfur, the pollution of sulfide is reduced, the elemental sulfur is recovered, and a higher value is created, so that the sulfate is more concerned by researchers, and the sulfate is also a hot spot of the current biological method research.
Meanwhile, the pure microorganism is utilized to make breakthrough progress on the heavy metal adsorption in a single or mixed heavy metal system, the research field is permeated into a plurality of fields such as molecular biology, biochemistry, environmental chemistry and the like, and precious metal can be recovered by means of bioleaching and the like while the microorganism is used for treating heavy metal pollution, so that the microorganism becomes one of research hot spots in the field of heavy metal treatment. For these two typical environmental pollutants, it would be a major breakthrough if the heavy metals could be remediated while sulfur pollution was being remediated. The inherent mechanism is still to be deeply explored to provide sufficient theoretical basis for economically and effectively using microorganisms for biological treatment and bioremediation. Natural generation and man-made manufacturing are the main sources of sulfides in wastewater. The sulfate reducing bacteria reduce high-valence sulfur into low-valence sulfur, and simultaneously increase the content of inorganic sulfur and organic sulfur in water, which is mainly caused by assimilation reaction and dissimilation reaction of organisms, wherein the assimilation reaction is a process of reducing oxidized sulfur into hydrogen sulfide by reducing oxidized sulfur into sulfhydryl for synthesizing biomacromolecules. The artificial increase of the content of hydrogen sulfide in water mainly comprises the rapid development of modern industry, the exploitation and refining of petroleum, the innovation of papermaking technology, the production of chemical fertilizer and pesticide, the desulfurization and purification of coal and the continuous development of chemical process, thus leading to the generation of the water-soluble H 2 S、HS - 、S2 - In the form of industrial waste water, which is converted into substances by ionization reactions in aqueous solutions.
Meanwhile, the first galvanization processing amount in the electroplating industry in China is mainly chromium salt, and meanwhile, chromate is widely used in operations such as copper part pickling, copper plating layer removal, aluminum part passivation, aluminum part electrochemical polishing, passivation after aluminum part oxidation and the like, and chromium plating accounts for a large part of the electroplating industry, so that chromium-containing wastewater is one of the main components of electroplating wastewater.
Cr (VI) is mainly CrO 4 2- And Cr (V) 2 O 7 2- Is in the form of strong oxidizing property, is very soluble in water and CrO 4 2- Can easily enter cells from specific channels on cell membranes, is reduced by substances such as intracellular glutathione, ascorbate, citrate and the like to generate Cr (III) compounds, and then is combined with a DNA chain to cause base pair mismatch, so that mutation and carcinogenesis are caused.
Bacillus cereus (Bacillus cereus FNXJ1-2-3, CGMCC No. 9683) was screened by the subject group and was patented by 2 patent publications (ZL 201510291867.1, ZL 201610427381.0). Researches show that the biological leaching agent composed of the strain can grow in a reaction liquid prepared from aluminum profile nickel plating sludge (the nickel content is 20 mg/L-1000 mg/L, and the aluminum content is 5-10 times of the nickel content) and a culture liquid, the biological leaching agent can tolerate the pH value of 4.0-7.0, and the nickel enrichment rate under the aerobic condition can reach 78.6% at most; the nickel enrichment under anaerobic conditions is up to 63.2%. This demonstrates that bioleaching agents exhibit excellent nickel enrichment and growth activity under both high acidity conditions as well as anaerobic or aerobic conditions. Therefore, the biological leaching agent has good application prospect in the biological purification of the aluminum profile nickel plating sludge and the preparation of a biological degradation agent or engineering bacteria for the aluminum profile nickel plating sludge.
Disclosure of Invention
The research of the researchers of the invention shows that the strain also has good power generation capacity and desulfurization capacity, and is hopeful to become an excellent strain for chromium removal and desulfurization.
In a first aspect, the invention discloses the use of bacillus cereus for desulphurisation and chromium and sulphur removal in water.
In some embodiments of the invention, the bacillus cereus is Bacillus cereus FNXJ1-2-3 with a preservation number of CGMCC No.9683.
In some embodiments of the invention, the sulfur is a sulfur ion.
In some embodiments of the invention, the chromium ion comprises at least one of a chromate and a dichromate.
In a second aspect of the present invention, there is disclosed a method for desulfurizing and chromium removing and desulfurizing in water using Bacillus cereus, comprising the step of culturing the Bacillus cereus in sulfur-containing or chromium-and sulfur-containing water.
In some embodiments of the invention, the method further comprises the step of adding a culture medium for the bacillus cereus.
In some embodiments of the invention, the temperature of the bacillus cereus is 20-40 ℃.
In some embodiments of the invention, the temperature of the bacillus cereus culture is 30-40 ℃.
In some embodiments of the invention, the temperature of the bacillus cereus culture is 35 ℃.
In some embodiments of the invention, the pH of the cultured Bacillus cereus is between 5.5 and 7.5.
In some embodiments of the invention, the culturing of bacillus cereus is for a period of 4-24 hours.
In some embodiments of the invention, the culturing of bacillus cereus is for a period of 12-24 hours.
In some embodiments of the present invention, the cultured Bacillus cereus has an inoculum size of (2-7). Times.10 9 And each mL.
In some embodiments of the present invention, the cultured Bacillus cereus is inoculated in an amount of (5-7). Times.10 9 And each mL.
In some embodiments of the invention, the bacillus cereus medium comprises a carbon source, a nitrogen source, a phosphorus source, and a pH of 7-8.
In some embodiments of the invention, the carbon source of the bacillus cereus medium is NaAC.
In some embodiments of the invention, the nitrogen source of the culture medium of bacillus cereus is KNO 3 And NH 4 Cl。
In some embodiments of the invention, the phosphorus source of the bacillus cereus medium is Na 2 HPO 4 And KH 2 PO 4 。
In some embodiments of the invention, the concentration of NaAC in the whole culture system is 0.7-0.9g/L.
In some embodiments of the invention, the KNO 3 The concentration of the NH in the whole culture system is 0.7-0.9g/L 4 The concentration of Cl in the whole culture system is 0.5-0.7g/L.
In some embodiments of the invention, the Na 2 HPO 4 The concentration of KH in the whole culture system is 1.0-1.5g/L 2 PO 4 The concentration in the whole culture system is 1.5-2.0g/L.
In some embodiments of the invention, the method further comprises the step of charging nitrogen to remove oxygen.
In some embodiments of the invention, the concentration of sulfur in the entire culture system is 25-100g/L.
In some embodiments of the invention, the chromium is present in a concentration of 20-40g/L throughout the culture system.
The technical effects are as follows:
the invention discloses a method for desulfurizing and removing chromium and desulfurizing in water by using bacillus cereus, which comprises the steps of using bacillus cereus Bacillus cereus FNXJ1-2-3 (with the preservation number of CGMCC No. 9683) obtained by screening and preserved, adding NaAC as carbon, and KNO 3 And NH 4 Cl is a nitrogen source, na 2 HPO 4 And KH 2 PO 4 The culture medium is a phosphorus source culture medium, and is filled with nitrogen to remove oxygen for culture, so that the culture temperature, the culture time, the inoculation amount and the concentration of each component of the culture medium are optimized.
The bacillus cereus disclosed by the invention can effectively remove sulfur and chromium in water.
Drawings
FIG. 1 shows the growth of bacteria at 12, 24h without chromium addition according to one embodiment;
FIG. 2 shows the sulfur residue at 12 and 24 hours of the bacteria of one embodiment;
FIG. 3 shows the sulfur residue at 12 and 24 hours of the bacteria of one embodiment;
FIG. 4 shows the degradation rate of sulfur and chromium by bacteria at 12h for one embodiment;
FIG. 5 shows the degradation rate of sulfur and chromium by bacteria at 12h for one embodiment;
FIG. 6 shows the degradation rate of sulfur and chromium by bacteria at 12h for one embodiment;
FIG. 7 shows the degradation rate of sulfur and chromium by bacteria at 12h for one embodiment;
FIG. 8 is a graph showing the degradation rate of sulfur and chromium by bacteria of one embodiment;
FIG. 9 is a graph showing the degradation rate of sulfur and chromium by bacteria at 12 hours according to one embodiment;
FIG. 10 shows the degradation rate of sulfur and chromium by bacteria at 12 hours for one embodiment;
FIG. 11 shows the degradation rate of sulfur and chromium by bacteria at 12 hours according to one embodiment.
Detailed Description
Other advantages and effects of the present invention will become apparent to those skilled in the art from the following disclosure, which describes the embodiments of the present invention with reference to specific examples. The invention may be practiced or carried out in other embodiments that depart from the specific details, and the details of the present description may be modified or varied from the spirit and scope of the present invention.
The examples and comparative examples are parallel runs of the same components, component contents, preparation steps, preparation parameters, unless otherwise specified.
In the following examples, the sulfur is a sulfur ion S 2- The chromium is chromium ion, and chromic acid radical CrO is used as the chromium ion 4 2- And dichromate Cr 2 O 7 2- In the form of (2), the molar ratio, calculated on Cr atoms, is 1:1.
in the following examples, a 100mL fermentation flask was charged with 20mL of medium, 1mL of sulfur-containing deionized water, and if necessary, 1mL of chromium-containing deionized water. 1mL of seed solution was inoculated.
In the following examples, the medium composition and the sulfur and chromium concentrations refer to the concentrations in the whole culture system (including medium, sulfur-containing deionized water, chromium-containing deionized water, inoculated bacterial fluids).
Example 1
Activation of 1.Bacillus cereus FNXJ1-2-3 and seed liquid preparation
Activating strains: the tube of strain 1 preserved in the laboratory at-70 ℃ is immediately placed in a water bath at 38-40 ℃ for rapid recovery and is properly and rapidly shaken until the internal ice is completely dissolved. And (3) taking a small amount of frozen tube bacterial liquid, transferring the frozen tube bacterial liquid onto an LB solid culture medium, and continuously transferring the frozen tube bacterial liquid into the LB solid culture medium for two times of activation.
Chromium removal desulfurization test of 2.Bacillus cereus FNXJ1-2-3
2.1 action of bacteria on Sulfur without chromium addition
Take 100mL fermentation flask and add NaAC:0.8g/L KNO 3 ：0.75g/L，NH 4 Cl:0.6g/L，Na 2 HPO 4 :1.2g/L，KH 2 PO 4 :1.8g/L, maintaining the pH at 7.5 and the temperature at 25 ℃. And nitrogen was sparged to remove oxygen. Adding 0, 20, 40, 60, 80, 100mg/L of sulfide ions into the fermentation bottles respectively, and adding bacterial liquid to make the concentration of bacterial liquid in the fermentation bottles be 2 x 10 x 9/mL.
The growth of the bacteria at 12, 24h without chromium is shown in FIG. 1. When the sulfur ion is contained, the effect of the growth of bacteria is best when the sulfur concentration is 40MG/L. The residual sulfur rate is shown in FIG. 2. When the sulfur concentration is 80mg/L, the residual rate of sulfur is the lowest, namely the degradation effect of bacteria is the best.
2.2 interaction of Sulfur and chromium without addition of bacteria
Take 100mL fermentation flask and add NaAC:0.8g/L KNO 3 ：0.75g/L，NH 4 Cl:0.6g/L，Na 2 HPO 4 :1.2g/L，KH 2 PO 4 :1.8g/L, maintaining the pH at 7.5 and the temperature at 25 ℃. And nitrogen was sparged to remove oxygen. The chromium concentration is controlled to be constant and 50mg/L, and the concentration of the sulfide ions is respectively 0, 25, 50, 75, 100, 150, 200, 250 and 300mg/L.
At 12h, the degradation rate of chromium and sulfur under the interaction of chromium and sulfur is shown in FIG. 3.
2.3 interaction of Sulfur and chromium in case of addition of bacteria
2.3.1 variation of degradation Rate of bacteria on Sulfur and chromium with a constant concentration of chromium ions and a variation of the concentration of Sulfur ions
Take 100mL fermentation flask and add NaAC:0.8g/L KNO 3 ：0.75g/L，NH 4 Cl:0.6g/L，Na 2 HPO 4 :1.2g/L，KH 2 PO 4 :1.8g/L, maintaining the pH at 7.5 and the temperature at 25 ℃. And nitrogen was sparged to remove oxygen. The sulfur ion concentration is 0, 25, 50, 75, 100mg/L and the chromium ion concentration is 30mg/L.
The degradation rate of the bacteria to sulfur and chromium is 12h, namely the degradation rate of sulfur and chromium after the bacteria are cultured for 12 h. The following is the same. See fig. 4. When the concentration of chromium ions is 30mg/L and the concentration of sulfur ions is 50mg/L, the bacteria have the best effect of degrading sulfur and chromium.
2.3.2 variation of the degradation Rate of bacteria on Sulfur and chromium with a constant concentration of Sulfur ions and a variable concentration of chromium ions
Take 100mL fermentation flask and add NaAC:0.8g/L KNO 3 ：0.75g/L，NH 4 Cl:0.6g/L，Na 2 HPO 4 :1.2g/L，KH 2 PO 4 :1.8g/L, sulfur concentration of 50mg/L and chromium concentration of 20, 25, 30, 35, 40mg/L, respectively. Adding bacteria liquid to make the concentration in the fermentation bottle be 2 x 10≡9/mL, and charging nitrogen gas to remove oxygen.
The degradation rate of the bacteria to sulfur and chromium for 12h is shown in figure 5. The bacteria have the best effect of degrading sulfur and chromium when the sulfur concentration is 50mg/L and the chromium concentration is 35mg/L.
2.4 the degradation rate of bacteria to sulfur and chromium is affected by temperature changes
Take 100mL fermentation flask and add NaAC:0.8g/L KNO 3 ：0.75g/L，NH 4 Cl:0.6g/L，Na 2 HPO 4 :1.2g/L，KH 2 PO 4 :1.8g/L, maintaining the pH at 7.5, sulfur concentration at 50mg/L and chromium concentration at 35mg/L. Adding bacteria liquid to make the concentration in the fermentation bottle be 2 x 10≡9/mL, and charging nitrogen gas to remove oxygen. Controlling the temperature to be 20, 25, 30, 35, 40DEG C. And taking 5 fermentation bottles without bacteria, and repeating the operation to obtain a zero sample.
The degradation rate of the bacteria on sulfur and chromium for 12h is shown in figure 6. The bacteria have the highest degradation rate of sulfur and chromium at 35 ℃.
2.5 degradation Rate of bacteria on Sulfur and chromium is affected by pH
Take 100mL fermentation flask and add NaAC:0.8g/L KNO 3 ：0.75g/L，NH 4 Cl:0.6g/L，Na 2 HPO 4 :1.2g/L，KH 2 PO 4 :1.8g/L, sulfur concentration of 50mg/L and chromium concentration of 35mg/L. Adding bacteria liquid to make the concentration in the fermentation bottle be 2 x 10≡9/mL, and charging nitrogen gas to remove oxygen. The pH was controlled to 5.5,6.0,6.5,7.0,7.5. And taking 5 fermentation bottles without bacteria, and repeating the operation to obtain a zero sample.
The degradation rate of the bacteria on sulfur and chromium for 12h is shown in Table 7. At pH 6.5, the bacteria have the highest degradation rate for sulfur and chromium.
2.6 the degradation rate of bacteria to sulfur and chromium is affected by time
Take 100mL fermentation flask and add NaAC:0.8g/L KNO 3 ：0.75g/L，NH 4 Cl:0.6g/L，Na 2 HPO 4 :1.2g/L，KH 2 PO 4 :1.8g/L, sulfur concentration of 75mg/L and chromium concentration of 35mg/L. Adding bacteria liquid to make the concentration in the fermentation bottle be 2 x 10≡9/mL, and charging nitrogen gas to remove oxygen. And taking 5 fermentation bottles without bacteria, and repeating the operation to obtain a zero sample.
The degradation rate of bacteria on sulfur and chromium is shown in Table 8. After about 12 hours from the start of degradation, the degradation rate of bacteria on sulfur and chromium tended to be smooth.
2.7 bacterial degradation of Sulfur and chromium is affected by the inoculum size of the bacteria
Take 100mL fermentation flask and add NaAC:0.8g/L KNO 3 ：0.75g/L，NH 4 Cl:0.6g/L，Na 2 HPO 4 :1.2g/L，KH 2 PO 4 :1.8g/L, sulfur concentration of 75mg/L and chromium concentration of 35mg/L. Each bacterial liquid is added to make the concentration of the bacterial liquid in a fermentation bottle be 2 x 10-9, 3 x 10-9, 4 x 10-9, 5 x 10-9, 6 x 10-9, 7 x 10-9/mL in sequence, and nitrogen is filled to remove oxygen.
Wherein 2 x 10And { fraction (A) } 9 represents 2×10 9 The remainder being the same.
The degradation rate of the bacteria on sulfur and chromium for 12h is shown in figure 9. When the inoculation amount of bacteria is 5 x 10 times 9/mL, the degradation rate of bacteria to sulfur and chromium is best.
2.8 the degradation rate of bacteria to sulfur and chromium is affected by carbon source
Adding KNO into 100mL fermentation bottle 3 ：0.75g/L，NH 4 Cl:0.6g/L，Na 2 HPO 4 :1.2g/L，KH 2 PO 4 :1.8g/L, sulfur concentration of 75mg/L and chromium concentration of 35mg/L. The concentration of each bacterial liquid in the fermentation bottle is 2 x 10≡9/mL. NaAC concentrations of 0.6,0.7,0.8,0.9,1.0g/L were added, respectively. And taking 5 fermentation bottles without bacteria, and repeating the operation to obtain a zero sample.
The degradation rate of the bacteria on sulfur and chromium for 12h is shown in figure 10. The carbon source has no obvious influence on the degradation rate of sulfur and chromium by bacteria.
2.9 the degradation rate of bacteria to sulfur and chromium is affected by nitrogen source
Take 100mL fermentation flask and add NaAC:0.8g/L KNO 3 ：0.75g/L，Na 2 HPO 4 :1.2g/L，KH 2 PO 4 :1.8g/L, sulfur concentration of 75mg/L and chromium concentration of 35mg/L. Each bacterial liquid is added to make the concentration in the fermentation bottle be 2 x 10-9/mL, and nitrogen is filled to remove oxygen. Adding NH in sequence 4 Cl 0.55,0.65,0.75,0.85,0.95g/L. And taking 5 fermentation bottles without bacteria, and repeating the operation to obtain a zero sample.
The degradation rate of the bacteria on sulfur and chromium for 12h is shown in figure 11. The nitrogen source has no obvious influence on the degradation rate of sulfur and chromium by bacteria.
3 measurement method
3.1 determination of chromium
1mL of the liquid to be measured is taken and placed in a 50mL colorimetric tube, and diluted with water until marked. Add 0.5mL sulfuric acid solution and 0.5mL phosphoric acid solution and shake well. Adding 2mL of color developing agent, shaking uniformly, adding 1mL of solution to a cuvette at a wavelength of 540nm after 5min, taking blank as a reference, and measuring absorbance.
3.2 determination of Sulfur
Taking 5 50mL colorimetric tubes with plugs, adding 10mL zinc acetate-sodium acetate solution respectively, taking 1mL to-be-measured solution respectively, transferring into the colorimetric tubes, slowly adding 5mL of N, N-dimethyl-p-phenylenediamine solution along the walls of the colorimetric tubes, immediately mixing uniformly, adding 0.5mL of ammonium ferric sulfate solution, standing for 10min, diluting with water to marked lines, and shaking uniformly. 1mL of the solution was added to the cuvette, and absorbance was measured at 665nm, with the blank as a reference.
While the preferred embodiments and examples of the present invention have been described in detail, the present invention is not limited to the above-described embodiments and examples, and various changes may be made within the knowledge of those skilled in the art without departing from the spirit of the present invention.
Claims (13)
1. A method for removing chromium and desulfurizing in water by using bacillus cereus, which is characterized by comprising the step of culturing bacillus cereus in water containing chromium and sulfur;
the bacillus cereus isBacillus cereusFNXJ1-2-3 with preservation number of CGMCC No.9683;
the sulfur is sulfur ion, and the chromium is chromium ion; the chromium ions comprise at least one of chromate and dichromate;
culturing bacillus cereus at 20-40 ℃;
culturing bacillus cereus at a pH of 5.5-7.5;
culturing the bacillus cereus for 4-24 hours;
the concentration of the sulfur in the whole culture system is 25-100g/L;
the concentration of the chromium in the whole culture system is 20-40g/L;
the inoculation amount of the cultured bacillus cereus is (5-7) multiplied by 10 9 And each mL.
2. The method of claim 1, further comprising the step of adding a medium for the bacillus cereus.
3. A method according to claim 1 or 2, characterized in that,
the culture is carried outThe inoculation amount of the bacillus cereus is (2-7) multiplied by 10 9 And each mL.
4. The method according to claim 3, wherein the temperature of the cultured bacillus cereus is 30-40 ℃;
and/or, the pH of the cultured bacillus cereus is 7.0-7.5;
and/or, the time for culturing the bacillus cereus is 12-24 hours.
5. The method according to claim 3, wherein the temperature of the cultured bacillus cereus is 35 ℃.
6. The method according to claim 1 or 2, wherein the culture medium of bacillus cereus comprises a carbon source, a nitrogen source, a phosphorus source, and has a pH of 7-8.
7. The method of claim 6, wherein the carbon source of the culture medium of bacillus cereus is NaAC.
8. The method according to claim 6, wherein the nitrogen source of the culture medium of Bacillus cereus is KNO 3 And NH 4 Cl。
9. The method according to claim 6, wherein the phosphorus source of the culture medium of Bacillus cereus is Na 2 HPO 4 And KH 2 PO 4 。
10. The method according to claim 7, wherein the concentration of NaAC in the whole culture system is 0.7-0.9g/L.
11. The method according to claim 8, characterized in that said KNO 3 The concentration of the NH in the whole culture system is 0.7-0.9g/L 4 The concentration of Cl in the whole culture system is 0.5-0.7g/L.
12. The method according to claim 9, wherein the Na 2 HPO 4 The concentration of KH in the whole culture system is 1.0-1.5g/L 2 PO 4 The concentration in the whole culture system is 1.5-2.0g/L.
13. The method of claim 1 or 2, further comprising the step of charging nitrogen to remove oxygen.
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