KR101366417B1 - Covering material for antimicrobial moist wound and Method of manufacturing the same - Google Patents

Abstract

One aspect of the present invention is characterized in that the chitosan coating layer is formed on the fabric member by immersion and drying of the water-soluble chitosan.
As another aspect of the invention, the first step of dissolving the water-soluble chitosan in distilled water; A second step of forming a coating material by coating the mixed aqueous solution on a fabric member; A third step of securing pores of the coated coating material; A fourth step of drying the coating material; A fifth step of spraying a crosslinking solution on the dried coating material; And a sixth step of drying the coating material.
This promotes wound healing of chitosan from the dressing material encoded by the water-soluble chitosan, and exhibits an excellent effect of preventing or inhibiting infection from the outside.

Description

Covering material for antimicrobial moist wound and method of manufacturing the same}

The present invention relates to an antimicrobial wound coating material using a natural material and a method for manufacturing the same. More specifically, the natural polymer that contributes to the improvement of antimicrobial power to aid in wound healing through a process of coating and drying chitosan, a natural polymer, on a foam dressing material. The present invention relates to an antimicrobial wound coating material using a substance and a method of manufacturing the same.

Skin is the body that has the largest surface area in the body and functions as an immune function. It protects against various harmful environments such as microorganisms, ultraviolet rays, and chemicals, and prevents dehydration and regulates body temperature by inhibiting water evaporation. It must play a role, be strong in physical stimuli, be resilient, and fulfill its role while the human body is living. Therefore, if a skin wound occurs, this important function is abnormal and the human body is out of balance.

The treatment of wounds is an important process that allows the patient to be treated by the biological process of restoring tissue that has been broken down as the basis of any trauma or surgery. The method of treating a wound can be divided into a method of treating a wound by maintaining a dry state such as a gauze dressing and a method of maintaining a wet state. In wet dressings that cover wounds and maintain a moist environment, the development over the last two decades has outpaced the development over the last hundred years. Clinical data fully demonstrate the safety and effectiveness of the wet environment provided by wet dressings in treating chronic wounds, which were considered acute and incurable wounds.

Wet dressings mainly include basic dressings such as hydrocolloids, hydrogels, polyurethanes and calcium alginates, antimicrobial dressings that upgrade them, and dressings containing growth factors.

In this regard, prior patents such as "Antibacterial dressing laminate and its manufacturing method" of Korean Unexamined Patent Publication No. 2010-0021108 and "Medical dressing containing antibacterial agent" of Korean Patent Publication No. 0994918 are known.

In addition, the current commercialized antimicrobial dressings were manufactured using nanotechnology to produce fine nanoparticles of silver such as nanocrystaline silver, which were electrically coated on polyurethane mesh fibers having a single layer structure, and similar technologies (US Patent Nos. 6,719,987, 6,087,549), i.e., as dry dressings, these products contain an excessive amount of silver and exhibit excellent antimicrobial activity, whereas they have a strong cytotoxicity against normal cells, and the silver itself is separated from the product. have. Therefore, the skin of the application site may be temporarily discolored, and there is an inconvenience in that it must be wetted with distilled water before use. Along with this, there is a shortage of absorption ability to absorb the exudate, which does not provide a wet environment. As such, products incorporating silver nanotechnology have structural problems such as uneven size and shape of nanoparticles, difficulty in controlling particle distribution, difficulty in controlling concentration and content of silver, and high manufacturing cost.

Therefore, economical antimicrobial wet dressing material is urgently required while providing effective antibacterial action and absorption of exudate at the same time. Especially, development of a new antimicrobial closure dressing material that can express specific ingredients and double skin regeneration ability is required. do.

The object of the present invention to focus on the above point, while having a sufficient antimicrobial action on various pathogenic bacteria while having minimal toxicity to the growth of normal cells, absorbing exudate without sticking to the wound surface to provide a wet environment At the same time, the purpose of the present invention is to provide an antimicrobial wound coating material and a method of manufacturing the same using natural materials to increase the effect of wound healing and to prevent or treat infection.

In order to achieve the above object, one aspect of the present invention is characterized in that the chitosan coating layer is formed through immersion and drying of a water-soluble chitosan on the fabric member.

In addition, according to the present invention, the water-soluble chitosan is characterized in that it has a molecular weight of 500 ~ 200,000 Da.

In addition, according to the present invention, the chitosan coating layer is characterized in that it contains a myriad of pores.

As another aspect of the invention, the first step of dissolving the water-soluble chitosan in distilled water; A second step of forming a coating material by coating the mixed aqueous solution on a fabric member; A third step of securing pores of the coated coating material; A fourth step of drying the coating material; A fifth step of spraying a crosslinking solution on the dried coating material; And a sixth step of drying the coating material.

In addition, according to the present invention, the first step is characterized by comprising a water-soluble chitosan in the range of 0.018 ~ 10w / v% in distilled water.

In addition, according to the present invention, the first step is characterized in that for selecting the molecular weight of the water-soluble chitosan in the range of 500 ~ 200,000 Da.

In addition, according to the present invention, the second step may be selected from spray coating, dip coating, flow coating, or spin coating. .

In addition, according to the present invention, the third step is characterized in that to form pores by operating 0.1 to 0.5 atm with an air injector.

In addition, according to the present invention, the fourth and sixth steps are characterized in that the hot air of 50 ~ 150 ℃ acts.

In addition, according to the present invention, the fifth step is characterized in that at least one of CaCl 2, TPP (Tripolyphospate) is used as the crosslinking solution.

In addition, according to the present invention is characterized in that the pores are formed by acting 0.1 to 0.5 atm between the fifth and sixth step.

According to the present invention as described above, it exhibits an excellent effect of promoting wound healing of chitosan from the dressing material encoded by the water-soluble chitosan, and preventing or suppressing infection from the outside.

1 is a flow chart showing the manufacturing process of the antimicrobial wound coating material according to the present invention
Figure 2 is a graph of the FT-IR measurement results showing the chitosan coating state according to the present invention
Figure 3 is a photograph measured by SEM after chitosan coating according to the present invention
Figure 4 is an illustration of applying the antimicrobial wound coating material according to the invention to the wound
Figure 5 is an illustration of applying the antimicrobial wound coating material according to the invention in the negative pressure treatment

Hereinafter, exemplary embodiments of the present invention will be described in detail with reference to the accompanying drawings.

The present invention relates to a technique for antimicrobial action on the skin wound of an infected or infected patient and to facilitate inhalation of exudate. In a humid environment, regenerated epithelial cells develop smoothly along the window, but in a dry environment, the regenerated epithelial cells do not develop along the window but progress along the way in the wet environment, making the path slower and wound healing inefficient. . In addition, substances involved in wound healing such as multinuclear leukocytes, macrophages, proteolytic enzymes, and cell growth factors contained in the effluent are discharged to the outside or dried in a dry environment, but do not play a role in a wet environment. Because it can be performed, wound healing proceeds efficiently.

According to one aspect of the invention, the coating member 50 is formed by forming a chitosan coating layer through the immersion and drying of a water-soluble chitosan on the fabric member. This coating material 50 is produced through a coating process described later.

At this time, the water-soluble chitosan of the present invention is characterized by having a molecular weight of 500 ~ 200,000 Da. The water-soluble chitosan preferably has a molecular weight of 500 to 200,000 Da, more preferably 5,000 to 100,000 Da. When the molecular weight is 500 Da or less, it is difficult to form a coating layer with a uniform thickness, and when the molecular weight is 200,000 Da or more, agglomeration occurs in the coating process.

On the other hand, the chitosan coating layer may include a myriad of pores. Forming pores on the coating layer is completed through the process described later.

The coating material 50 of the present invention, which is prepared from chitin / chitosan, which is a natural polymer, has an extremely superior advantage as compared with products manufactured from conventional synthetic polymers. Since the coating material 50 has better human compatibility than the existing product, the healing effect of the wound itself is superior, and the adsorbing ability of heavy metals and heavy metals, which cannot be seen in the product made from synthetic polymers, is largely detoxifying effect. Can be exercised. Unlike simple wounds, cuts, and burns, wounds generated by biochemical warfare can be healed only by effectively removing harmful substances attached to the skin. The coating material 50 of the present invention can simultaneously perform treatment and detoxification. .

According to another aspect of the present invention, there is provided a manufacturing method including a process consisting of the first step to the sixth step.

In the first step of the present invention, the water-soluble chitosan is put into distilled water and dissolved. A water-soluble chitosan coating solution is obtained using water-soluble chitosan or a derivative thereof as an active ingredient.

At this time, the first step may include a water-soluble chitosan in distilled water in the range of 0.018 ~ 10w / v%. That is, 0.018-10 g of chitosan is dissolved in distilled water to produce a coating agent 12, which is a 100 mL chitosan solution. Of course, the solvent is not limited to distilled water and purified water may be used. In mass production, the dispersion 12 may be mixed with the chitosan aqueous solution to produce a coating agent 12.

On the other hand, the first step is preferably to select the molecular weight of the water-soluble chitosan in the range of 500 ~ 200,000 Da. This is a range that causes the formation (quality) of the chitosan coating layer as described above. Of course, the molecular weight of the water-soluble chitosan is more preferably 5,000 ~ 100,000 Da in terms of uniform antibacterial and productivity.

In the second step of the present invention, the water-soluble chitosan coating solution is coated on a fabric member to form a coating material. In Figure 1, the fabric member 10 means foam dressing, but is not necessarily limited thereto. The coating 12 produced by mixing is adsorbed onto the fabric member 10 and coated.

In this case, the second step may be selected from a spray coating method, a dip coating method, a flow coating method, or a spin coating method. In consideration of the quality and productivity according to the use of the finished product coating material 50 may be selected from one or a plurality of the above methods.

The third step of the present invention goes through the process of securing the pores of the coated coating material. Although the coating material 50, which is the finished product of the present invention, provides a wet environment, a minimum pore should be secured to prevent necrosis of the skin tissue.

At this time, the third step is to form pores by acting 0.1 to 0.5 atm with the air injector 20. The air injector 20 is a means for injecting air supplied from a pneumatic device (not shown) to a plurality of nozzles. If the air pressure is too low, the formation of pores is not smooth. If the air pressure is too high, the coating 12 may be scattered, resulting in unevenness of the coating layer.

In the fourth step of the present invention, the coating material is dried. Drying of the coating material may proceed naturally with drying at room temperature, but in consideration of mass productivity, it is necessary to warm it to an appropriate temperature.

At this time, the fourth step is preferably to operate the hot air of 50 ~ 150 ℃ using the dryer (30). Too low a drying temperature increases the airborne by drying, while too high results in a weakening of the antimicrobial quality. In particular, if the temperature exceeds 200 ℃ the physical properties of the foam dressing and chitosan coating layer is changed to increase the defective rate.

In the fifth step of the present invention, the crosslinking solution 14 is sprayed onto the dried coating material. In the fifth step, at least one of CaCl 2 and TPP (Tripolyphospate) is used as the crosslinking solution 14. Appropriate crosslinking process increases the adhesion of chitosan to the fabric member (10).

In the sixth step of the present invention, the coating material is dried. The drying process using the dryer 30 is the same as the above-described fourth step.

At this time, it is preferable to form pores by operating 0.1 to 0.5 atm between the fifth and sixth steps. Forming pores in the coating material 50 is performed in the same manner as the above-described third step.

The antimicrobial foam dressing material provided by this method can absorb and store more than 10 times the moisture of its own weight to maintain a moist environment when attached to the wound to provide an effective environment for wound healing, and chitosan also helps to heal wounds, Provides antimicrobial effect against.

<Examples>

Hereinafter, the present invention can be described in more detail through the following specific examples. However, the present invention is not limited by these examples.

Examples 1-8

In the process of dissolving water-soluble chitosan in 1 L of distilled water, various content ranges were set and eight conditions showing significant antimicrobial properties were selected through repeated experiments. That is, the concentration of water-soluble chitosan in the chitosan solution was maintained at 0.018, 0.037, 0.075, 0.125, 0.25, 0.5, 1, 10% (w / v), respectively, and stored at room temperature.

The solution thus prepared is immersed in the coating agent 12 in a foam dressing (length: 10 cm, width 10 cm, thickness 3 cm) at room temperature to be absorbed to form a sufficient coating layer, after which the foam dressing is taken out to secure air pores. After a hot air drying process at about 50 ° C. for 24 hours, an antimicrobial foam dressing coated with a water soluble chitosan was prepared.

<Experimental Example 1>

The antimicrobial activity of the antimicrobial foam dressings prepared in the above manner was measured according to K. J 4206: 2008 shake flask method. It was carried out in the strain, the results are shown in Table 1 below. If the water-soluble chitosan is included in the range of 0.018 ~ 10w / v%, antimicrobial activity can be generated, but in terms of mass production products, the range of 0.075 ~ 10w / v% is more preferred.

Sample name Antimicrobial activity (%) Remarks Example 1 (concentration 0.018% (w / v)) 2.0% Example 2 (concentration 0.037% (w / v)) 4,5% Example 3 (concentration 0.075% (w / v)) 9,2% Example 4 (concentration 0.125% (w / v)) 20,4% Example 5 (Concentration 0.25% (w / v)) 50,2% Example 6 (Concentration 0.5% (w / v)) 70,1% Example 7 (Concentration 1% (w / v)) 98,9% Example 8 (Concentration 10% (w / v)) 99.9%

<Experimental Example 2>

In order to confirm the coating according to the embodiment was analyzed by FT-IR. The foam dressing coated in Example 1 was mixed with KBr in a ratio of 100: 1, prepared as a pellet, and a sample was prepared by removing water under reduced pressure at 60 ° C. for 12 hours. The sample was measured using an FT-IR spectrometer (Shimadzu, FT-IR 8700, Japan) and the results are shown in FIG. After coating the chitosan to the foam dressing, the amine peak and the absorption peak of amide I appearing at 1550 cm &lt; -1 &gt; Formation of the coating layer was confirmed by confirming the characteristic peak of chitosan (see FIG. 3).

In FIG. 4, the coating | covering material 50 is affixed on the skin S, and the negative pressure film 46 is affixed on the outside. In Fig. 5, the exudates can be discharged by inserting the suction head 44 and applying negative pressure through the tube 42 extending outwardly.

The indication of the antimicrobial coating material 50 according to the present invention is suitable for treating acute wounds such as burns and chronic wounds such as pressure sores and diabetic foot ulcers. In the case of 2 or more severe burns and chronic wounds, the body's protective function is impaired, and a large amount of exudate is continuously generated along with the risk of infection or risk of infection by the bacteria. In addition, not only the depth of the wound is deepened by the infection, but also the recent increase in the average life expectancy of the elderly patients is rapidly increasing and various chronic wounds are rapidly increasing. The present invention provides a coating material 50 of a typical therapeutic method that can treat and prevent infection by bacteria and absorb exudate using an external antimicrobial agent.

For reference, chitosan to be applied as an antimicrobial compound is a kind of polysaccharide existing in nature, a compound obtained by deacetylating chitin contained in the cell walls of microorganisms such as crab, shrimp shell, squid bone, mold and bacteria. It has been used in various industrial fields since the mid 1980s. In the past, chitosan was mainly used in the field of wastewater treatment such as flocculants, heavy metal adsorbents, dye wastewater purification agents, and agricultural fields such as soil modifiers, insecticides, plant antiviral agents, and pesticides. The range of food and beverage applications, health and hygiene applications, cosmetic applications, textile-related applications, and pharmaceutical applications is expanding. In particular, the use of chitosan in wound healing agents, artificial skin, pharmacologic materials, blood coagulants, artificial kidney membranes, biodegradable surgical sutures, and antimicrobial materials has been reported since the 1990s. More specifically, German Patent Nos. 1,906,155 and 1,906,159, which are almost the first patents related to chitin and chitosan-related wound dressings, disclose that pulverized chitin powder has an excellent effect on wound recovery. 3,632,754 and U.S. Patent No. 3,914,413 disclose that chitin promotes wound healing and has the property of degrading to lysozyme and thus can be physiologically soluble. Japanese Laid-Open Patent Publication No. 57-143508 proposes a gauze made from chitin fiber, which only shows the physical properties of the gauze. In Japanese Patent Laid-Open Publication No. 5-92925, chitosan and cotton, chitin and cotton were used for the treatment of burns, indicating that the effect of protecting the wound and alleviating pain is excellent. Was dispersed in water at a concentration of 1.5% (w / v), poured onto a polystyrene film and lyophilized to obtain a sponge form.

In addition, U. S. Patent No. 4,651, 725 discloses a method for producing a wound dressing consisting of a chitin fiber nonwoven fabric; U.S. Patent No. 4,699,135 discloses a method for producing a wound coating consisting of a nonwoven fabric or fibrous sheet of fibrous chitin body; Korean Patent Laid-Open No. 2001-79260 discloses a method for manufacturing a clinical pharmaceutical coating material using high culture chitosan short fibers; Korean Patent Laid-Open No. 2001-67991 discloses a method for producing a functional fiber containing chitosan; Korean Patent Laid-Open Publication No. 2000-14189 discloses a method for manufacturing a nonwoven fabric for wound treatment using chitin and chitosan alginic acid. However, these conventional gauze dressings are easy to absorb the wound secretion, but they do not have protection against infections such as bacteria from the outside and keep the wound dry so that treatment is delayed, and the dressing material adheres to the wound surface and is not easy to exchange. Not only are there problems with neoplastic damage and pain. In addition, because the exudates occur in the early stages of healing, there is a disadvantage that the dressing material must be changed several times a day.

On the other hand, according to the present invention, it is easy to prepare an antimicrobial wet dressing which can secure economic efficiency while simultaneously providing effective antibacterial action and absorption of exudate.

It will be apparent to those skilled in the art that various modifications and variations can be made in the present invention without departing from the spirit and scope of the invention as defined by the appended claims. It is therefore intended that such variations and modifications fall within the scope of the appended claims.

10: fabric member 12: coating agent
14: crosslinking liquid 20: air gun
30: dryer 42: tube
44: suction head 46: film for negative pressure
50: coating material S: skin

Claims (11)

delete delete delete A first step of dissolving a water-soluble chitosan in distilled water to obtain a water-soluble chitosan coating solution;
A second step of forming a coating material by coating the water-soluble chitosan coating solution on a fabric member;
A third step of securing pores in the coated coating material by actuating 0.1 to 0.5 atm with an air injector;
A fourth step of drying the coating material;
A fifth step of spraying a crosslinking solution on the dried coating material; And
The sixth step of drying the coating material; Method of producing an antimicrobial wound coating material using a natural material comprising a. 5. The method of claim 4,
The first step is a method for producing an antimicrobial wound coating material using a natural material, characterized in that it comprises a water-soluble chitosan in the range of 0.018 ~ 10w / v% in distilled water. 5. The method of claim 4,
The first step is a method for producing an antimicrobial wound coating material using a natural material, characterized in that for selecting the molecular weight of water-soluble chitosan in the range of 500 ~ 200,000 Da. 5. The method of claim 4,
The second step is an antimicrobial wound using a natural material, characterized in that selected from spray coating, dip coating, flow coating, or spin coation Method of manufacturing coating material. delete 5. The method of claim 4,
The fourth and sixth step is a method for producing an antimicrobial wound coating material using a natural material, characterized in that the hot air of 50 ~ 150 ℃ acts. 5. The method of claim 4,
The fifth step is a method for producing an antimicrobial wound coating material using a natural material, characterized in that at least one of CaCl2, TPP (Tripolyphospate) as a crosslinking solution. 5. The method of claim 4,
Method of producing an antimicrobial wound coating material using a natural material, characterized in that the pore is formed by acting 0.1 to 0.5 atm with an air injector between the fifth and sixth step.

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