KR101329051B1 - Method for preparing probiotics using stevia - Google Patents

Abstract

Provided is a probiotic production method using stevia.
Probiotic production method according to an embodiment of the present invention comprises the steps of crushing stevia hay; Obtaining a liquid extract from the stevia hay crushed matter; Mixing the degreased soybean meal pulverized product with the obtained extract, to prepare a solid catalyst; Diluting the prepared solid catalyst with water, and then mixing molasses, stevia vinegar solution, raw yeast and useful microbial cell powder with the diluted water; And activating and aging the mixed solution.

Description

Method for preparing probiotics using stevia {Method for preparing probiotics using stevia}

The present invention relates to a method for producing a probiotic using stevia, and more particularly, to a method for producing a probiotic that can be used for the purpose of deodorization, antibacterial, and the like from stevia.

Stevia is a chrysanthemum and a perennial plant and is known as a sweet, 200-fold sweet and non-toxic natural sweetener crop.

Since stevia has little change in physical properties due to heating, it is used as a sweetener directly in a tea or a bean curd as a raw leaf, and Korean Patent Registration No. 10-0620854 discloses a method for producing Stevia extract. The useful microorganisms isolated from the stevia extract fermentation broth and the effective microorganisms (EM) obtained as a result of the identification are disclosed in Journal of Life Science, Vol. 10, No. 6 (October 2006).

However, a method for efficiently extracting useful substances such as probiotics and the like from such useful stevia has not yet been disclosed.

Accordingly, the problem to be solved by the present invention is to provide a method for producing a useful probiotic from stevia.

In order to solve the above problems, the present invention comprises the steps of crushing stevia hay; Obtaining a liquid extract from the stevia hay crush; Mixing the obtained extract with the defatted soybean meal pulverized product to prepare a solid catalyst; Diluting the prepared solid catalyst with water, mixing molasses, stevia raw liquid, raw yeast, and useful microbial cell powder into the diluted water; And it provides a method for producing a probiotic from stevia, comprising the step of activating and aging the mixed solution.

According to one embodiment of the present invention, the activity is carried out by exposing the mixture to oxygen and light for 24 hours at 40 degrees Celsius per hour for 15 minutes, for 2 days, and the aging is carried out at a temperature of 30 degrees Celsius And the mixture is stirred for 5 days while exposing the mixture to oxygen and light 24 times a day for 5 minutes. The defatted soybean has a particle size of 250 mesh or less, and the liquid extract obtained from the hay stevia is extracted at 150 brix or more .

In addition, the useful microbial cell powder is crushed and crushed stevia hay; Heating the crushed stevia hay in a liquid phase to obtain a liquid stevia extract; Concentrating the liquid Stevia extract in vacuo at a temperature of 40 degrees to obtain a Stevia concentrate; Mixing the stevia concentrate and the stevia dry powder with seeds, molasses, sugar and salt, followed by culturing at a temperature of 40 to 50 degrees Celsius for 5 to 7 days; Fermenting / aging the cultured mixture at a temperature of 25 to 35 degrees Celsius for 50 to 60 days; And the useful microbial stock solution is prepared by centrifugation and filtration through a microfiltration network to isolate / dry the cells to extract the cell powder.

The present invention is based on a method of completely differentiating from a conventional technique, that is, a method in which a microbial powder is obtained by separating a useful microbial liquid phase, a solid catalyst is extracted from a stevia hay concentrate and a defatted soybean, Probiotic agents are prepared. Accordingly, the present invention uses both hay and stevia as a raw material, and particularly, the useful microbial cell powder obtained from Stevia is used as a catalyst to produce a probiotic agent. As a result, the production period is shortened and a solid catalyst is used, Production costs are reduced compared to products. Therefore, the probiotics manufactured from Stevia according to the present invention can be utilized as deodorizing, antibacterial and antibiotic materials.

1 is a step for explaining a method for obtaining a liquid phase from hay stevia according to an embodiment of the present invention.
Figure 2 is a step of the useful microbial cell powder catalyst manufacturing process according to another embodiment of the present invention.
3 is a step diagram illustrating a grinding process of the degreased soybean meal which constitutes one component of the solid catalyst according to the present invention.
Figure 4 is a step diagram of a solid catalyst manufacturing process according to another embodiment of the present invention.

In order to fully understand the present invention, operational advantages of the present invention, and objects achieved by the practice of the present invention, reference should be made to the accompanying drawings and the accompanying drawings which illustrate preferred embodiments of the present invention.

Hereinafter, the present invention will be described in detail with reference to the preferred embodiments of the present invention with reference to the accompanying drawings. However, the present invention can be implemented in various different forms, and is not limited to the embodiments described. In order to clearly describe the present invention, parts that are not related to the description are omitted, and the same reference numerals in the drawings denote the same members.

In the present invention, a solid catalyst is prepared from hay stevia and defatted soybean meal, and then the solid catalyst is mixed with stevia raw material, raw yeast, useful microbial cell powder, and fermented to produce a probiotic. The probiotic prepared according to the present invention has excellent deodorizing effect by using the characteristics of stevia, and has an effect of degrading environmental hormones, livestock manure and food waste.

Hereinafter, each step process will be described in detail with reference to the drawings.

Example  One

Hay Stevia Liquid Extract

1 is a step for explaining a method for obtaining a liquid phase from hay stevia according to an embodiment of the present invention.

Referring to Figure 1, first, the stevia hay is ground so that the length of one side is 5 mm or less.

Thereafter, the pulverized product of stevia was put into a bag of 5 kg per one extraction bag, and the bag was sealed. The sealed bag was put in water and heated at a temperature of 100 ° C. for 6 hours to extract the liquid extract twice (120 kg / water 1 ton).

Thereafter, the remaining liquid phase obtained from the extractor is pressurized and extracted by a press to be combined with the conventional liquid extract. Thereafter, in order to minimize the change in physical properties, the obtained liquid extract was concentrated under vacuum at 20 degrees Celsius until it was concentrated to 80 Brix (about 50 kg), and the vacuum concentrated liquid extract was concentrated to 160 Brix (about 25 kg). Dry with spray dry until dry. Through the above process, according to the present invention, a concentrated form of Stevia liquid extract is obtained, and the obtained liquid extract is mixed with the defatted soybean meal crumbs described below to form a solid catalyst.

Example  2

Useful microbial cell powder

Example  2-1

Powder catalyst preparation

First, stevia is harvested from the biological state, and only the leaves are collected and washed with water and dehydrated. In one embodiment of the present invention, in addition to stevia, antioxidant plants such as Seize or Green tea were used with Stevia, where the antioxidant plants were mixed with Stevia at 3% by weight.

The dehydrated sage and stevia fresh green leaf are placed in a Pyrex container and lyophilized in liquid nitrogen. The moisture content of the fresh green leaf is 10% or less through freeze-drying.

Put sage and stevia leaves lyophilized as above into a grinder and grind. Thereafter, the pulverized product is passed through a predetermined mesh (150 mesh) sieve several times to obtain stevia, which is the first filtrate in powder form. At this time, the solid component that has not passed through the sieve may be used as a raw material for the hay process.

Thereafter, the extrusion process proceeds. In the extrusion process, first, an inlet and an outlet of the first filtrate are contained in a separated glass container, and an air pressure generator capable of keeping the filtrate in a scattered state is installed at an inlet, For the shortening of the process, install a 250 mesh and a 300 mesh screen with a proper space in two. Further, in order to store the filtrate having passed through the double-sided filter, a filtration storage container having an air outlet of 500 mesh is installed at the outlet side, and then the filtrate is repeatedly extruded by using air pressure. The extrusion method first passes through 250 mesh several times, then removes the large particles and 250 mesh sieve that did not pass the 250 mesh, and then passed the powder particles passed through the 250 mesh through 300 mesh again to obtain fine particles of different sizes. . The extruded second filtrate at a level of 1/700 to 1,000 of the fresh-leaf mass is obtained, and the filtrate (second filtrate) in the fine powder is heated in a liquid phase.

Upon examination of the heating process, first, the extruded second filtrate is mixed with a mole / alcohol mixture (alcohol concentration 10%), which is three times the volume of the second extract. The heating temperature is maintained at 75 to 85 DEG C for a time sufficient to remove all of the alcohol components, for example 2 hours, whereby a Stevia-based catalyst is prepared.

Thereafter, the obtained liquid catalyst is lyophilized with liquid nitrogen to obtain a dried powder catalyst having a water content of 10% or less.

Example  2-2

Useful Microbial Stock Preparation

First, stevia hay is crushed and crushed. This yields a stevia hay powder, which is then placed in a fibrous bag, sealed, placed in a sealed fibrous bag in water, and heated to a temperature of 75 to 85 degrees Celsius for 8 hours to produce a hay-based liquid extract. The water was 1000 kg for 200 kg stevia hay powder. Thereafter, the fibrous bag is pressurized with a press to obtain a residual extract, which is then mixed with a conventional extract to obtain a final extract. Again, the concentration process is carried out for the obtained liquid extract, in one embodiment of the present invention, the concentration process is heated to 40 degrees Celsius in vacuum until the liquid extract obtained from 1 ton of water and 200 kg of powder is concentrated to 700 kg That's the way it is.

Therefore, in Example 2-1, a catalyst is obtained from stevia fresh grass, and in this example, a liquid concentrate is obtained from hay.

Then, the dry powdery catalyst and the liquid concentrate are mixed to perform active culturing. In one embodiment of the present invention, the cultured material is prepared by mixing 700 kg of the crude liquid extract, 50 g of the catalyst of Example 2-1, molasses (300 kg) A mixture of traces of bacteria, sugar, and salt in a trace amount, and the culturing / mixing is carried out in a manner of stirring for 5 to 7 days under oxygen conditions at a temperature of 40 to 50 degrees Celsius. Thereafter, the active / cultivated material is fermented / aged at 25 to 35 degrees Celsius for 50 to 60 days, thereby obtaining a stock solution of useful microorganisms from Stevia.

Example  2-3

Cell powder  Produce

Figure 2 is a step of the useful microbial cell powder catalyst manufacturing process according to another embodiment of the present invention.

Referring to FIG. 2, the useful microbial stock solution of Example 2-2 was filtered once through a centrifuge and a microfiltration network, and separated into a pure secondary metabolite liquid phase and an extract (sludge including bacteria) of the useful microorganisms. The product liquid phase is used as a raw material for supplementary feed, and the cell powder as an extract is used in the probiotic preparation process according to the present invention. At this time, the extracted cell powder may be dried.

Example  3

Defatted soybean meal  smash

3 is a view illustrating a pulverization process of defatted soybean meal constituting one component of the solid catalyst according to the present invention.

Referring to FIG. 3, the defatted soybean meal is dried to a moisture content of 20% or less. Both the drying process and the thermal drying method of 60 ° C. or less can be used. Thereafter, the dried defatted soybean meal is finely pulverized to a size of 300 mesh or less, and the pulverized defatted soybean meal is repeatedly filtered several times to a 250-mesh sieve. The powder passed in the filtration process is applied to the solid catalyst production process described below, and the powder that has not passed is reused in the pulverization process.

Example  4

Solid catalyst manufacturing

4 is a step diagram of a solid catalyst production process according to another embodiment of the present invention.

Referring to FIG. 4, 12.5 kg of the stevia hay concentrate prepared in Example 1, 15 kg of the defatted soybean meal powder prepared in Example 3, and 20 kg of glucose (total 40 kg) are mixed. Thus, a solid catalyst necessary for the production of a probiotic agent is prepared. To prevent changes in physical properties, it is stored at -1 to -2 degrees Celsius. The stored solid catalyst is preferably used within 6 months.

Example  5

Fermentation process

The present invention mixes and ferments the solid catalyst prepared in Example 4 and the stevia raw vinegar extract-containing material to obtain a stevia-based probiotic to be prepared in the present invention.

FIG. 5 is a step diagram of a fermentation process according to one embodiment of the present invention.

5, 40 kg of the solid catalyst prepared in Example 4 was diluted in 900 L of water, and 50 kg of molasses, 5 L of stevia liquid phase and 2 kg of raw yeast were added to the diluted solution, and the stock solution of beneficial microorganism (EM) prepared in Example 2 Is mixed with 500 g of the cell powder. The stock solution of the useful microorganism used in the present invention is the substance obtained by mixing the Stevia raw material catalyst with the liquid stevia concentrate and then fermenting it as described in Example 2. [

Referring to FIG. 5 again, the solid catalyst and molasses of Example 3, liquid extract obtained from raw grass, raw yeast (EM) and useful microbial (EM) cell powder mixture were mixed with oxygen 24 times a day for 15 minutes at 40 degrees Celsius. Activate for 2 days, exposing to light. At this time, molasses 50 kg + EM stock 2L. Then, the mixture is aged and fermented by stirring with oxygen and light for 5 days at a temperature of 30 degrees Celsius for 5 minutes per hour for 5 days. Through the above process, a probiotic having an effect of deodorization, antibiotics and antibacterial is prepared.

Hereinafter, the rest of the configuration is the same as the above embodiment and a detailed description thereof will be omitted.

While the present invention has been particularly shown and described with reference to exemplary embodiments thereof, it is to be understood that the invention is not limited to the disclosed exemplary embodiments, This is possible.

Claims (4)

Steps to Shred Stevia Hay
Obtaining a liquid extract from the stevia hay crushed matter;
Mixing the degreased soybean meal pulverized product with the obtained extract, to prepare a solid catalyst;
Diluting the prepared solid catalyst with water, and then mixing molasses, stevia vinegar solution, raw yeast and useful microbial cell powder with the diluted water; And
The method of producing a probiotic from stevia, characterized in that it comprises the step of activating and aging the mixed solution. The method of claim 1,
The activity is performed by exposing the mixed solution to oxygen and light 24 times a day for 15 minutes at 40 degrees Celsius for 2 days, and the aging process is performed for 5 minutes per hour for 5 minutes per hour at a temperature of 30 degrees Celsius. The method of producing a probiotic from stevia, characterized in that proceeding by stirring for 5 days while exposing the mixture. The method of claim 1,
The defatted soybean meal has a particle size of 250 mesh or less, and the liquid extract obtained from the hay stevia is concentrated to 150 brics or more, the method for producing a probiotic from stevia. According to claim 1, wherein the useful microbial cell powder is
Crushing and crushing stevia hay;
Heating the crushed stevia hay in a liquid phase to obtain a liquid stevia extract;
Concentrating the liquid Stevia extract in vacuo at a temperature of 40 degrees to obtain a Stevia concentrate;
Mixing the stevia concentrate and the stevia dry powder with seeds, molasses, sugar and salt, followed by culturing at a temperature of 40 to 50 degrees Celsius for 5 to 7 days;
Preparing a useful microbial stock solution from stevia, wherein the cultured mixture is obtained by fermentation / maturation of the cultured mixture at a temperature of 25 to 35 degrees for 50 to 60 days; And
Method for producing a probiotic from stevia, characterized in that the useful microbial stock is filtered by centrifugation and microfiltration to separate / dry the cells and extract the cell powder.

Images