KR101325960B1 - OsHCI1 gene from rice for enhancing high temperature stress resistance of plant and uses thereof - Google Patents

Abstract

The present invention is derived from rice OsHCI1 (Oryza sativa Heat Cold Induced 1) A method of controlling resistance to high temperature stress of a plant, comprising transforming a plant cell with a recombinant vector comprising a gene, the OsHCI1 A method for producing a transformed plant with controlled resistance to high temperature stress, comprising the step of transforming plant cells with a recombinant vector comprising a gene, a transformed plant with controlled resistance to high temperature stress produced by the method and Its seeds and said OsHCI1 It provides a composition for controlling high temperature stress resistance of a plant comprising a gene.

Description

OsHCI1 gene from rice for enhancing high temperature stress resistance of plant and uses approximately}

The present invention relates to OsHCI1 gene derived from rice and its use to increase the high temperature stress resistance of plants, and more particularly, to OsHCI1 derived from rice (Oryza sativa Heat Cold Induced 1) A method of controlling resistance to high temperature stress of a plant, comprising transforming a plant cell with a recombinant vector comprising a gene, the OsHCI1 A method for producing a transformed plant with controlled resistance to high temperature stress, comprising the step of transforming plant cells with a recombinant vector comprising a gene, a transformed plant with controlled resistance to high temperature stress produced by the method and Its seeds and said OsHCI1 It relates to a composition for controlling high temperature stress resistance of a plant comprising a gene.

Under natural and agricultural conditions, plants must constantly withstand stress. Some environmental factors (eg, hot or cold) can be very stressful in a very short time, while others (eg soil water content or mineral nutrients) can take a long time to stress plants. In general, environmental stresses affecting plants can be in the form of climate-related stress, air pollution stress, chemical stress and nutritional stress. Examples of climate-related stresses include drought, floods, frost, low temperatures, high temperatures, excessive light and insufficient light. Air pollution stress can be in the form of carbon dioxide, carbon monoxide, sulfur dioxide, NOx, hydrocarbons, ozone, ultraviolet light and acid rain. Chemical stress can result from the application of pesticides, fungicides, herbicides and heavy metals. Nutritional stress can be caused by fertilizers, micronutrients and macromutrients.

Susceptible species suffer cold damage at temperatures that are too low to grow normally and not too low to freeze. Such damage typically occurs in species of tropical or subtropical origin. When cooled, decolorization or lesions appear on the leaves, which give these leaves a water-soaked appearance. When the roots cool down, the plants can wither. On the other hand, freezing temperatures and subsequent formation of ice crystals in plants can be fatal if the ice crystals expand to protoplasts or are maintained for a long time.

Stress can also be caused by other excessive temperatures, and almost all plants cannot survive at high temperatures. If the cells or tissues of the higher plant are not dehydrated or grown, these cells can survive better at higher temperatures than hydrated cells, feeder cells, and cells during growth. Actively growing tissues are rarely viable at temperatures above 45 ° C.

Meanwhile, Korean Patent No. 0742193 describes the production of an environmental stress resistant plant by separating a novel environmental stress resistant transcription factor from barley and introducing the transcription factor into a plant, and in Korean Patent Registration No. 0742194 A method of increasing the environmental stress resistance of a plant by introducing a vector containing the AIA gene, which is a rod-derived environmental stress resistance regulatory gene, into the plant is overexpressed. However, as described in the present invention, the OsHCI1 gene derived from rice and its use for increasing the high temperature stress resistance of plants have not been found.

The present invention was derived from the above requirements, the present inventors confirmed that OsHCI1 gene expression of rice plants increased under high temperature stress conditions, transgenic Arabidopsis plants overexpressing the OsHCI1 gene show high temperature stress resistance By confirming, the present invention was completed.

In order to solve the above problems, the present invention is rice-derived OsHCI1 (Oryza sativa Heat Cold Induced 1) Provides a method for controlling the resistance of plants to high temperature stress, comprising the step of transforming a plant cell with a recombinant vector comprising a gene.

In addition, the present invention is OsHCI1 Provided is a method for producing a transgenic plant having controlled resistance to high temperature stress, comprising transforming plant cells with a recombinant vector comprising a gene.

In addition, the present invention provides a transgenic plant and its seed whose controlled resistance to high temperature stress produced by the method.

In addition, the present invention is OsHCI1 It provides a composition for controlling high temperature stress resistance of a plant comprising a gene.

Rice-derived OsHCI1 according to the present invention Genes were strongly induced under high temperature stress conditions and transgenic Arabidopsis plants overexpressing the OsHCI1 gene showed high temperature stress resistance. Therefore, the OsHCI1 gene is expected to be useful for the development of transgenic plants resistant to high temperature stress.

1 shows OsHCI1 upon abiotic stress and plant hormone treatment Expression patterns of genes and genes related thereto are shown. OsSalT , OsbZIP23 , OsLIP19 And Hsp90 -1 was used as marker genes for abiotic stress treatment, OsSalT , OsPBZ1 , OsPR1b and OsERF3 was used as a marker gene for each plant hormone treatment. C and T represent control and stressed samples, respectively.
2 shows the results of confirming the intracellular location of the OsHCI1 protein.
Figure 3 shows the yeast two-hybrid screening results for the analysis of matrix proteins that interact with OsHCI1 protein.
4 shows six substrate proteins that interact with OsHCI1 protein.
5 shows expression patterns of protein coding genes that interact with OsHCI1 in rice under high and low temperature stress conditions. C and T represent control and stressed samples, respectively.
6 shows the intracellular locations of six proteins, OsPSA7, OsPGLU1, OsbHLH065, OsGRP1, OsPOX1 and Os14-3-3, which interact with OsHCI1 in rice.
7 shows BiFC analysis of six substrate proteins, OsPSA7, OsPGLU1, OsbHLH065, OsGRP1, OsPOX1 and Os14-3-3, which interact with OsHCI1 in rice under high temperature stress conditions.
8 shows that OsHCI1 has E3 ubiquitin ligase function and mediates ubiquitination with OsPGLU1, OsbHLH065, OsGRP1 and OsPOX1 proteins in vitro.
9 shows that OsHCI1 mediates nuclear-cytoplasmic trafficking.
10 shows the high temperature resistant phenotype of 35S :: OsHCI1-EYFP. Seedlings of 35S :: EYFP (EV) and 35S :: OsHCI1-EYFP T ₃ transgenic plants (3 independent lines) were grown on agar plates in light conditions for 7 days, heated at 38 ° C., 90 min, 24 After cooling for 2 hours at 45 ° C., it was heated for 3 hours (acquired thermotoleracne) or at 45 ° C. for 60 minutes (basal thermotolerance). A, 7 independent Col-0 / 35S :: OsOSHCI1 T ₃ RT-PCR analysis of transgenic plants, control wild type (WT) and empty vector (EV); Phenotypes of B, empty vector (EV) and three independent OsHCI1 overexpressing plants were treated with high temperature stress at various temperatures. Image was taken 5 days after heat stress treatment; C, Plant survival relative to empty vector (EV) in the same plate was measured on day 5 after heat stress treatment.

In order to achieve the object of the present invention, the present invention is a rice-derived OsHCI1 (Oryza sativa Heat Cold Induced 1) Provides a method for controlling the resistance of plants to high temperature stress, comprising the step of transforming a plant cell with a recombinant vector comprising a gene.

Method according to an embodiment of the present invention is rice-derived OsHCI1 Recombinant vector containing genes were transformed into plant cells and OsHCI1 It is characterized by increasing the plant's resistance to high temperature stress by overexpressing the gene, but not limited thereto.

In the method according to an embodiment of the present invention, the high temperature may be 40 ° C. or higher, preferably 40-50 ° C., more preferably 45 ° C., but is not limited thereto.

In the method according to an embodiment of the present invention, the OsHCI1 gene may be preferably composed of the nucleotide sequence of SEQ ID NO: 1. In addition, variants of the above nucleotide sequences are included within the scope of the present invention. Specifically, the gene has a base sequence having a sequence homology of at least 70%, more preferably at least 80%, even more preferably at least 90%, most preferably at least 95% with the nucleotide sequence of SEQ ID NO: 1, respectively. It may include. "% Of sequence homology to polynucleotides" is ascertained by comparing the comparison region with two optimally aligned sequences, and a portion of the polynucleotide sequence in the comparison region is the reference sequence for the optimal alignment of the two sequences (I. E., A gap) relative to the < / RTI >

The term "recombinant" refers to a cell in which a cell replicates a heterologous nucleic acid, expresses the nucleic acid, or expresses a protein encoded by a peptide, heterologous peptide or heterologous nucleic acid. The recombinant cell can express a gene or a gene fragment that is not found in the natural form of the cell in one of the sense or antisense form. In addition, the recombinant cell can express a gene found in a cell in its natural state, but the gene has been modified and reintroduced intracellularly by an artificial means.

In the present invention, the OsHCI1 gene sequence can be inserted into a recombinant expression vector. The term "recombinant expression vector" means a bacterial plasmid, a phage, a yeast plasmid, a plant cell virus, a mammalian cell virus, or other vector. In principle, any plasmid and vector can be used if it can replicate and stabilize within the host. An important characteristic of the expression vector is that it has a replication origin, a promoter, a marker gene and a translation control element.

Expression vectors comprising OsHCI1 gene sequences and appropriate transcriptional / translational control signals can be constructed by methods well known to those of skill in the art. Such methods include in vitro recombinant DNA technology, DNA synthesis techniques, and in vivo recombination techniques. The DNA sequence can be effectively linked to appropriate promoters in the expression vector to drive mRNA synthesis. The expression vector may also include a ribosome binding site and a transcription terminator as a translation initiation site.

A preferred example of the recombinant vector of the present invention is a Ti-plasmid vector capable of transferring a so-called T-region to a plant cell when present in a suitable host, such as Agrobacterium tumefaciens. Other types of Ti-plasmid vectors (see EP 0 116 718 B1) are currently used to transfer hybrid DNA sequences to plant cells or protoplasts in which new plants capable of properly inserting hybrid DNA into the plant's genome can be produced have. A particularly preferred form of the Ti-plasmid vector is a so-called binary vector as claimed in EP 0 120 516 B1 and U.S. Patent No. 4,940,838. Other suitable vectors that can be used to introduce the DNA according to the invention into the plant host include viral vectors such as those that can be derived from double-stranded plant viruses (e. G., CaMV) and single- For example, from non -complete plant virus vectors. The use of such vectors may be particularly advantageous when it is difficult to transform the plant host properly.

The expression vector will preferably comprise one or more selectable markers. The marker is typically a nucleic acid sequence having a property that can be selected by a chemical method, and includes all genes capable of distinguishing a transformed cell from a non-transformed cell. Examples include herbicide resistance genes such as glyphosate or phosphinothricin, kanamycin, G418, bleomycin, hygromycin, and chloramphenicol. Resistance gene, aadA gene, and the like, but are not limited thereto.

In the recombinant vector of the present invention, the promoter may be, but is not limited to, CaMV 35S, actin, ubiquitin, pEMU, MAS, histone promoter, Clp promoter. The term "promoter " refers to the region of DNA upstream from the structural gene and refers to a DNA molecule to which an RNA polymerase binds to initiate transcription. A "plant promoter" is a promoter capable of initiating transcription in plant cells. A "constitutive promoter" is a promoter that is active under most environmental conditions and developmental conditions or cell differentiation. Constructive promoters may be preferred in the present invention because the choice of transformants can be made by various tissues at various stages. Thus, constitutive promoters do not limit selectivity.

In the recombinant vectors of the present invention, conventional terminators can be used, for example nopalin synthase (NOS), rice α-amylase RAmy1 A terminator, phaseoline terminator, Agrobacterium tumefaciens ( Agrobacterium tumefaciens) Terminator of the octopine gene, and the rrnB1 / B2 terminator of E. coli, but are not limited thereto. Regarding the need for terminators, it is generally known that such regions increase the certainty and efficiency of transcription in plant cells. Therefore, the use of a terminator is highly desirable in the context of the present invention.

The method of carrying the vector of the present invention into the host cell may be carried out by microinjection, calcium phosphate precipitation, electroporation, liposome-mediated transfection, DEAE-dextran treatment, gene bombardment or the like. Can be injected.

In addition, the present invention is derived from OsHCI1 rice Transforming the plant cell with a recombinant vector comprising the gene; And it provides a method for producing a transformed plant is controlled resistance to high temperature stress comprising the step of regenerating the plant from the transformed plant cells.

Method according to an embodiment of the present invention is rice-derived OsHCI1 Recombinant vector containing genes were transformed into plant cells and OsHCI1 It is characterized by increasing the plant's resistance to high temperature stress by overexpressing the gene, but not limited thereto.

Preferably, the OsHCI1 gene may be composed of the nucleotide sequence of SEQ ID NO: 1.

The method of the invention comprises the step of transforming a plant cell with a recombinant vector according to the present invention, the transformant is, for example, Agrobacterium tyumeo Pacific Enschede may be mediated by (Agrobacterium tumefiaciens). In addition, the method of the present invention comprises regenerating a transgenic plant from the transformed plant cell. Any of the methods known in the art can be used for regeneration of transgenic plants from transgenic plant cells.

Transformed plant cells must be regenerated into whole plants. Techniques for the regeneration of mature plants from callus or protoplast cultures are well known in the art for a number of different species (Handbook of Plant Cell Culture, Vol. 1-5, 1983-1989, Momillan, N. Y.).

In addition, the present invention provides a transgenic plant and its seed whose controlled resistance to high temperature stress produced by the method.

Preferably, the transgenic plant and its seeds are transgenic plants and their seeds with increased resistance to high temperature stress.

Preferably, the plant may be a dicotyledonous plant, more preferably Arabidopsis, potato, eggplant, tobacco, pepper, tomato, burdock, garland chrysanthemum, lettuce, bellflower, spinach, chard, sweet potato, celery, carrot, buttercup, It may be a dicotyledonous plant, such as parsley, Chinese cabbage, cabbage, cat radish, watermelon, melon, cucumber, pumpkin, gourd, strawberry, soybean, mung bean, kidney bean, pea, and most preferably, it may be a Arabidopsis plant, but is not limited thereto. .

In addition, the present invention comprises a rice sequence OsHCI1 consisting of the nucleotide sequence of SEQ ID NO: 1 It provides a composition for controlling high temperature stress resistance of plants, including genes. The composition is OsHCI1 consisting of the nucleotide sequence of SEQ ID NO: 1 as an active ingredient Genes, which are capable of controlling (preferably increasing) the high temperature stress resistance of the plant by transforming the gene into the plant.

Hereinafter, the present invention will be described in detail by way of examples. However, the following examples are illustrative of the present invention, and the present invention is not limited to the following examples.

Example  One. Abiotic  When processing stress and plant hormones OsHCI1 Gene expression patterns

Among the RING finger protein coding genes, molecular biological analysis was performed by selecting Os10g30850 (GenBank Accession No.) gene whose mRNA was prominent under high temperature stress. Two main rice plants were treated with high temperature (45 ℃), low temperature (4 ℃), drought, salt (250mM NaCl) and plant hormone epsis acid (ABA, 0.1mM), jasmonic acid (JA, 0.1mM), salicylic acid (SA, 1mM) ), Ethylene (50 uL L ^-1 ) was treated hourly. The treated plant leaves were triturated with liquid nitrogen and total RNA was extracted using Trizol (Invitorogen). The total RNA extracted was synthesized the first strand cDNA using reverse transcriptase, and quantified based on the OsACTII gene. Quantified cDNA was confirmed using a marker gene that increases the expression level of the gene under each stress condition, and then OsHCI1 Semi-quantitative RT-PCR was performed using gene specific primers. The expression was increased at high temperatures (45 ° C.) and low temperatures (4 ° C.), specifically abiotic stresses, and was named OsHCI1 (Oryza sativa Heat Cold Induced 1). In the four plant hormone treatments, it increased up to 12 hours and gradually decreased thereafter (FIG. 1).

Example  2. OsHCI1  Protein Intracellular  Location verification

In order to confirm the intracellular location of OsHCI1 , the Agrobacterium was transformed by cloning the OsHCI1 gene in the binary vector pBIN35S :: EGFP vector. The transformed bacteria were incubated at 28 ° C. in the dark for 16 hours and subjected to agro-infiltration analysis using tobacco leaves about 1 month old. After 3 days, the tobacco leaves were cut to a size of 0.5 cm, and then intracellular location was observed using a confocal microscope. As a result, the 35S :: EYFP used as a control (FIG. 2A) showed fluorescence signals in the nucleus and cytoplasm, and the 35S :: OsHCI1-EGFP was found to move rapidly along the cytoskeleton in numerous dots in the cell. (Figures 2B and D). When compared with other experimental results, it was confirmed that these fluorescent signals were similar to Golgi, and co-transformation with 35S :: G-rk-mCherry, a Golgi marker protein distributed at the Arabianpsis Biological Resource Center (ABRC) OsHCI1 was located in the Golgi apparatus within the cell) (Fig. 2E). The fluorescence signal was observed by treating the tobacco leaves expressing OsHCI1 protein at 45 ° C. for 1 hour based on the mobility of the protein and the specific expression pattern at high temperature stress confirmed by RT-PCR. Interestingly, it was confirmed that signals located throughout the cells were moved and expressed around the nucleus by heat treatment (FIG. 2C).

Example  3. OsHCI1  Analysis of Substrate Proteins That Interact with Proteins

A Y2H (Yeast Two-Hybrid) screen was performed to find substrate proteins that interact with OsHCI1. The total length coding sequence of OsHCI1 gene was inserted into pGBKT7-BD vector loaded with GAL DNA-binding domain, transformed into Y2H-Gold yeast strain, and used as bait. CDNA was synthesized using brine-treated rice, inserted into a pGADT7-rec vector loaded with a GAL4 active domain, and transformed into a Y187 yeast strain to prepare a library. Cultured for 24 hours at 30 ℃ using a yeast mating method was plated in DDO / X / A (-Leu-Trp, 40ug / ml, X-α-Gal, 70 ng / ml Aureobasidin A) medium. Approximately 280 clones were obtained through the primary screen and transferred to QDO / X / A (-Leu-Trp-Ade-His, 40 ug / ml, X-α-Gal, 70 ng / ml Aureobasidin A) medium. Screen was performed. Finally, 24 positive clones were identified (FIG. 3A), among which OsPSA7 (Os01g59600, 20S proteasome subunit α7) showing strong X-α-GAL activity, OsPGLU1 (Os03g53800, Periplasmic beta-glucosidase), OsbHLH065, (Os04g14570, Six genes were selected: basic / Helix-Loop-Helix transcription factor), OsGRP1 (Os05g02770, Glycin-rich cell wall structural protein), OsPOX1 (Os07g48020, Peroxidase) and Os14-3-3 (14-3-3 protein). Total length CDS was obtained via RT-PCR and then inserted into the pGADT7 vector. Clones obtained by cotransforming the six genes inserted into the pGBKT7-OsHCI1 and pGADT7 vectors into the Y2H Gold yeast strain were dropped into DDO medium and QDO / X / A medium to confirm protein-protein interaction (FIG. 4B). BD-murine p53 and AD-SV40 large T-antigen were used as a positive control, and BD-lamin and AD-SV40 large T-antigen were used as a negative control.

RT-PCR was performed to confirm the expression patterns of the six genes that interact with OsHCI1 at high and low temperatures. Experimental method is the same as in Example 1, four genes including transcription factors of the six genes showed the opposite expression pattern to OsHCI1. In addition, the six genes showed the same expression pattern for both high and low temperature stress (FIG. 5).

Agrobacterium was transformed by inserting six genes into pBIN35S :: DsRed2 to find the association with OsHCI1 by identifying the intracellular locations of the six genes that interact with OsHCI1. Agro-infiltration using tobacco leaves was observed under confocal microscopy. Experimental method is the same as in Example 1. Fluorescent signals of OsPGLU1, OsbHLH065 and OsGRP1-DsRed2 were observed only in the nucleus, while OsPSA7, OsPOX1 and Os14-3-3 were observed in the cytoplasm or cytoplasmic nucleus (FIG. 6). This result suggests that OsHCI1 migrates to the nucleus at high temperature and interacts with at least three proteins located in the nucleus to transfer the intracellular positions of the nuclear proteins into the cytoplasm.

BiFC (Bimolecular fluorescence complementation) analysis was performed to reconfirm the interaction between the six genes identified through Y2H and the OsHCI1 gene in plant cells. OsHCI1 gene was inserted into the 35S-HA-SPYCE (M) vector, and six genes were inserted into the 35S-c-myc-SPYNE (R) 173 vector to transform Agrobacterium. Each clone was incubated at 28 ° C. for 16 hours and then infiltrated into tobacco leaves in six combinations simultaneously. In all six genes, YFP signal was strongly confirmed. Interestingly, only when injected with OsPGLU1-, OsbHLH065- and OsGRP1-DsRed2, the signal was observed in the nucleus, but the BiFC result injected simultaneously with OsHCI1 was observed in the nucleus and cytoplasm. It became. These results demonstrate the possibility that OsHCI1 can shift the intracellular positions of the genes that it interacts with.

Example  4. OsHCI1 of Ubiquitination  analysis( ubiquitination assay )

In the next step, in vitro ubiquitination assay was performed to confirm whether OsHCI1 plays the role of E3 ligase. The coding region of OsHCI1 gene was amplified by PCR and inserted into pMAL-c5X vector. MBP-OsHCI1 and MBP vectors were transformed into E. coli BL21 (DE3) and purified using amylose resin. AtUBC10 and atUBC11 used as E2 were inserted into the pET-28a-c (+) vector and transformed into E. coli, and purified using Ni-NTA purification system. Purified MBP-OsHCI1 (250 ng) and yeast E1 (50 ng), Arabidopsis E2 (250 ng), 10 ug bovine ubicutin were ubiquitin reaction buffer (50 mM Tris-HCl, pH 7.5, 10 mM MgCl2, 0.05 mM ZnCl2, 1 mM ATP, 0.2 mM DTT, 10 mM phosphocreatine, creatine kinase 0.1 unit) and sampled over time. In FIG. 8A, the ubiquitin polychain was formed in the lane where E1, E2, and ubiquitin were all reacted, confirming that the OsHCI1 protein had E3 ligase activity. In addition, by measuring the reaction time it was confirmed that the poly chain is formed over time. Three nuclear proteins and one cytoplasmic protein that interact with OsHCI1 were used to test whether OsHCI1 used these proteins as substrates (CF in FIG. 8). His-Trx was attached to four genes and expressed in E. coli and purified using a Ni-NTA purification system. The ubiquitination assay was performed to confirm that OsHCI1 and four purified cross-linking proteins were ubiquitized using OsHCI1-interacting protein as a substrate. Mono ubiquitination was formed in three nuclear proteins, and the polyphosphoryl chain was confirmed in the OsPOX1 protein located in the cytoplasm. The results suggest that OsHCI1 attaches mono ubiquitin to nucleoproteins to induce post translational modification of matrix proteins, thereby shifting the position of proteins located in the nucleus to the cytoplasm.

OsPGLU1, OsbHLH065 and OsGRP1-DsRed constructs and 35S :: OsHCI1-EYFP were used to confirm that OsHCI1 genes located in the Golgi apparatus and fast-moving genes in the cell move genes that interact with them, particularly proteins located in the nucleus, into the cytoplasm. Simultaneously, the cells were injected into tobacco cells to observe the fluorescence signal. When expressed alone, as shown in FIG. 9, the proteins whose signals were identified only in the nucleus (BD of FIG. 6) were observed simultaneously in the cytoplasm and the nucleus. You can see that you are performing the function to move.

Example  5. OsHCI1  High Temperature Stress Tolerance Analysis of Overexpressed Arabidopsis Plants

In order to confirm how the molecular cytological function of the OsHCI1 protein actually affects the high temperature stress of the plant, a transgenic Arabidopsis is expressed in which OsHCI1 is overexpressed. The 35S :: OsHCI1-EYFP vector was transformed into Agrobacterium and then inserted into the Arabidopsis through a floral dip method. In the seven independent T ₃ lines, the expression level was confirmed by RT-PCR, and the line with the strongest expression was selected to carry out the high temperature stress resistance experiment (FIG. 10A). As a control, Arabidopsis transformed with EYFP alone was used. The high temperature stress was divided into basal thermotolerance and acquired thermotolerance. Survival in basal thermotolerance after one hour treatment at 45 ° C was not significantly different from the control group. However, in the experiment pre-heat treatment for 38 minutes at 38 ℃ it was confirmed that obtained a high resistance of 55-60% or more compared to the control (Fig. 10 B and C). In conclusion, the OsHCI1 protein, an rice E3 ligase, is believed to confer resistance to acquired thermotolerance by shifting the proteins located in the nucleus to the cytoplasm, and the OsHCI1 gene, which confers resistance to such high temperature stress, is not a model plant. It could be used as a material for genetic breeding of climate change-response crops.

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Claims (12)

A method of controlling the resistance of plants to high temperature stress, comprising the step of transforming a plant cell with a recombinant vector comprising a rice-derived OsHCI1 (Oryza sativa Heat Cold Induced 1) gene consisting of the nucleotide sequence of SEQ ID NO: 1. The method of claim 1, wherein the rice-derived OsHCI1 Overexpressing the gene in plant cells, thereby increasing the plant's resistance to high temperature stress. delete Transforming a plant cell with a recombinant vector comprising a rice-derived OsHCI1 (Oryza sativa Heat Cold Induced 1) gene consisting of the nucleotide sequence of SEQ ID NO: 1; And
A method for producing a transgenic plant having controlled resistance to high temperature, comprising the step of regenerating a plant from the transformed plant cell. The method according to claim 4, wherein the rice-derived OsHCI1 Overexpressing the gene in plant cells to produce a transgenic plant having increased resistance to high temperature stress. delete Transgenic plant with controlled resistance to high temperature stress produced by the method of claim 4. 8. The plant of claim 7, wherein the plant has increased resistance to high temperature stress. 8. The transgenic plant of claim 7, wherein the plant is a dicotyledonous plant. 10. The transgenic plant of claim 9, wherein the dicotyledonous plant is Arabidopsis thaliana. Seeds of plants according to claim 7. Rice-derived OsHCI1 consisting of the nucleotide sequence of SEQ ID NO: 1 (Oryza sativa Heat Cold Induced 1) A composition for controlling high temperature stress resistance of a plant, comprising a gene.

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