KR101259499B1 - Compositions for improving blood circulation comprising fat-soluble ingredients of ginseng - Google Patents

Abstract

PURPOSE: A composition for improvement of blood circulation including ginseng fat-soluble components is provided to improve a blood circulation effect by decreasing neutral fat and LDL-cholesterol level and increasing HDL-cholesterol level when intaking the composition with supplementary agents for improving blood circulation. CONSTITUTION: An extraction method of ginseng root(fine root) fat-soluble components comprises following steps: a step of putting ginseng root into a super critical extracting container; a step of extracting unsaturated fatty acid of the ginseng root fat-soluble components by contacting ginseng root and carbon dioxide which is super critical fluid under the temperature of 40-60 deg. C and the pressure of 300-350bar; and a step of obtaining the ginseng root of fat-soluble components contained in the extracted result. The step of extracting unsaturated fatty acid is conducted under 10-20 ml/min fluid speed of the supercritical fluid. The unsaturated fatty acid is: docosanoic acid, eicosanoic acid, heneicosanoic acid, dodecanoic acid, heptadecanoic acid, hexadeccanoic acid, octadecanoaic acid, octanoic acid, pentadecanoic acid, tetradecanoic acid, tricosanoic acid, nonadecanoic acid, hexanoic acid and decanoic acid; or 11-eicosenoic acid, 11,13-eicosadienoic acid, 13-docosenoic acid, 9,12,15-octadecatrienoic acid, 9,12-octadecadienoic acid, 9-hexadecenoic acid, 9-octadecenoic acid, 11-octadecenoic acid, 7,10-hexadecadienoic acid and 6,9,12-octadecatrienoic acid.

Description

Composition for Improving Blood Circulation Comprising Fat-soluble Ingredients of Ginseng

The present invention relates to a blood circulation improving composition comprising a ginseng fat-soluble component.

Herbal herbal medicines such as ginseng, Hwanggi, and McMoon-dong are widely used for the purpose of alleviating and healing diseases. Among them, ginseng has been used as the most precious herbal material in the Far East for many years. Ginseng is the broadest concept that includes all ginseng, white ginseng, red ginseng, wild ginseng, camphor, rice ginseng, original ginseng, and taegeuk ginseng. The history of ginseng is the representative health functional food of Korea, which contains the efficacy of ginseng in a number of documents, and still ranks first among the health functional foods. With the recent westernization of dietary lifestyles, the improvement of living standards, and the generalization of well-being, consumers' food choices have been diversified, advanced, and health-oriented. Therefore, development demand for more diverse products is expected to increase for ginseng products, the representative health food of our country.

Ginseng contains 50-70% of carbohydrates, 10-15% of crude protein, 3-8% of irradiated phosphonine, and 3-7% of ash.The main bioactive components are saponin, phenolic, polyacetylene, Alkaloid components, polysaccharides, and the like are known.

Many extraction methods have been studied to extract various active ingredients including ginsenosides from ginseng. Traditional extraction method is a heating extraction method using a solvent such as water, ethyl alcohol. This extraction method takes a long time and has a disadvantage of low extraction efficiency. Extraction methods of active ingredients developed until recently are mainly concentrated on the extraction of ginseng saponins. Although ginseng fat-soluble components contain various compounds useful for the human body, the use efficiency is very low, so it is urgent to prepare their use methods.

Supercritical extraction using carbon dioxide is a useful method for the extraction of fat-soluble substances. Recently, extraction methods using the same have been developed. Supercritical extraction using carbon dioxide as a supercritical fluid has a low critical temperature to prevent thermal denaturation, is nonflammable, nontoxic, inexpensive and readily available, and, above all, has a generally safe advantage. Existing methods of extracting materials using water, methanol, ethanol, hexane and the like have to co-exist with the extraction solvent, so the solvent must be removed in one step, whereas the supercritical extraction method does not require such a procedure. It can be simplified.

In the present invention, it is intended to provide a blood circulation improving composition and a food composition using the same technology and the development of an extraction technique that can extract the fat-soluble components contained in ginseng in the maximum yield.

Numerous papers and patent documents are referenced and cited throughout this specification. The disclosures of cited papers and patent documents are incorporated herein by reference in their entirety, so that the level of the technical field to which the present invention belongs and the contents of the present invention are more clearly explained.

The present inventors earnestly researched to develop a technology that can extract fat-soluble active ingredients such as eicosanoic acid having excellent functionality in addition to saponin, which is the main active ingredient of ginseng, and as a result, supercritically extracted ginseng oil-soluble at specific temperature and pressure conditions The extract containing the component was found to have an excellent blood circulation improvement effect to complete the present invention.

It is therefore an object of the present invention to provide a method for extracting ginseng fat-soluble components.

Another object of the present invention is to provide a pharmaceutical composition comprising the ginseng fat-soluble component extracted by the method of the present invention.

Another object of the present invention is to provide a food composition comprising the ginseng fat-soluble component extracted by the method of the present invention.

Another object of the present invention to provide a food composition comprising a ginseng fat-soluble component and blood circulation improving adjuvant.

Other objects and advantages of the present invention will become more apparent from the following detailed description of the invention, claims and drawings.

According to one aspect of the present invention, the present invention provides a method for extracting ginseng fat-soluble component comprising the following steps:

(a) placing ginseng in a supercritical extraction vessel;

(b) extracting ginseng fat-soluble components by contacting ginseng and a supercritical fluid under conditions of a temperature of 30-70 ° C. and a pressure of 100-400 bar; And

(c) obtaining a ginseng fat-soluble component contained in the extraction result of step (b).

The present inventors earnestly researched to develop a technology that can extract fat-soluble active ingredients such as eicosanoic acid having excellent functionality in addition to saponin, which is the main active ingredient of ginseng, and as a result, supercritically extracted ginseng oil-soluble at specific temperature and pressure conditions It was found that extracts containing the components had excellent blood circulation improving effects.

For convenience of description, the method of the present invention is described herein as applied to ginseng. However, the present invention can be applied to a variety of ginseng, such as white ginseng, red ginseng, black ginseng, rice ginseng, raw ginseng, taegeuk ginseng, wild ginseng, camphor ginseng, and goat ginseng, and it is apparent to those skilled in the art that the present invention is included in the scope of the present invention. As the ginseng used in the present invention, a variety of known ginsengs can be used, and Korean ginseng ( Panax ginseng ), Gyegi ( P. quiquefolius ), Jeonchisam ( P. notoginseng ), Bamboo ginseng ( P. japonicus ), and three leaf ginseng ( P trifolium ), Himalayan ginseng ( P. pseudoginseng ), and Vietnamese ginseng ( P. vietnamensis ).
As used herein, the term "ginseng root" refers to the remaining root of the ginseng except for the body of ginseng, and "ginseng body" refers to the body except for the root of the ginseng.

As used herein, the term "extract" has the meaning commonly used as a crude extract in the art, but broadly includes a fraction additionally fractionating the extract. That is, extracts containing ginseng fat-soluble components include not only those obtained by using a supercritical fluid, but also those obtained by additionally applying a purification process thereto. For example, fractions obtained by passing the extract through an ultrafiltration membrane having a constant molecular weight cut-off value, separation by various chromatography (manufactured for separation according to size, charge, hydrophobicity or affinity), etc. The fraction obtained through the purification method is also included in the ginseng fat-soluble component extract of the present invention.

Extract of ginseng fat-soluble component used in the present invention may be prepared in a powder state by an additional process such as distillation under reduced pressure and freeze drying or spray drying.

The method of the present invention will be described in detail in each step as follows:

Step (a): adding ginseng to a supercritical extraction vessel

The production method of the present invention first comprises the step of processing the ginseng into a suitable form and placing it in a supercritical extraction container.

The ginseng used in the present invention may be grown or commercially available, and may be used by appropriately cutting dry, frozen or natural materials.

According to the method of the present invention, the ginseng is ground or cut to a suitable size in order to maximize the contact area with the solvent.

Step (b): Extracting Ginseng Fat-Soluble Ingredients

Next, a supercritical fluid is applied to the supercritical extraction container of step (a) to extract the ginseng fat-soluble component.

As used herein, the term “supercritical fluid” refers to a fluid that is above the critical temperature and pressure, and has a unique characteristic that is different from conventional solvent extraction methods. Substances in the supercritical state change pressures near their critical point, and many properties such as density, viscosity, diffusion coefficient, and polarity are continuously changed very largely from gas to liquid. When the carbon dioxide of the liquid is put into a closed container and heated, the boundary of the gas-liquid interface gradually disappears as the temperature and pressure increase. Supercritical fluid is a heavy fluid that fills a closed space like a gas. Supercritical fluid technology takes advantage of supercritical fluids such as high dissolving power, fast mass transfer and heat transfer, low viscosity, high diffusion coefficient and fast penetration into micropores due to low surface tension. The supercritical fluid diffuses into the medium faster than the liquid solvent and can extract the constituents from the medium with the solvent-like solvent strength that it still has. In addition, the fluid in the supercritical region can be freely controlled in a wide range from gas to liquid by changing the temperature and pressure (density, diffusion coefficient, thermal conductivity, partial molar volume, solubility, hydrogen bonding strength, etc.) This has advantages over general solvent extraction with only temperature variables.

Supercritical fluids used in the extraction process are carbon dioxide, ammonia, nitrogen, oxygen, helium, SO ₂ , ethane, ethylene, propane, propylene, sulfur hexafluoride, nitrous oxide, chlorotrifluoromethane, monofluoromethane , Xenon or combinations thereof, preferably the supercritical fluid is carbon dioxide.

Since carbon dioxide is the main component of the atmosphere, it can be safely and easily obtained. In addition, carbon dioxide can be purchased at a relatively low cost. Since the amount of carbon dioxide used in the present invention does not adversely affect the atmosphere, carbon dioxide is environmentally friendly compared to other supercritical fluids. Oxygen dioxide also has the advantage of being non-flammable and non-explosive, leaving virtually no residue after evaporation. On the other hand, since carbon dioxide changes in polarity according to temperature and pressure, it can be adjusted to have polarity and nonpolarity by adjusting temperature and pressure as necessary. By varying the temperature and pressure it is possible to change the solvent properties of the carbon dioxide fluid, which allows the separation of several components in a single solvent system.

In the extraction method of the present invention, the ginseng is supercritically extracted under the conditions of temperature 30-70 ° C., pressure 100-400 bar, and flow rate 1-20 ml / min of the supercritical fluid, more preferably, temperature 35-. It can be carried out under the conditions of 65 ℃ and pressure 220-380 bar, flow rate of the supercritical fluid 10-20 ml / min. Ginseng fat-soluble components extracted under these conditions are particularly characterized by excellent blood circulation improvement.

One of the greatest features of the present invention is to provide optimum supercritical extraction conditions according to the site of use of ginseng raw materials. Therefore, by selecting and performing the optimum supercritical extraction conditions according to the site of use of ginseng raw materials in the above-described temperature and pressure range, excellent extraction yield is obtained. When the use site of the ginseng raw material is the ginseng root, the supercritical extraction condition of the ginseng is preferably a temperature of 35-65 ° C, more preferably 35-55 ° C, even more preferably 35-45 ° C. ego; Preferably pressure 200-400 bar, more preferably 250-400 bar, even more preferably 300-400 bar, even more preferably 320-380 bar. According to a specific embodiment of the present invention, the ginseng fat-soluble component supercritical extraction conditions of the ginseng root is a temperature of 40 ℃ and 350 bar pressure.

When the use site of the ginseng raw material is a ginseng body, the supercritical extraction condition of the ginseng is preferably a temperature of 40-65 ° C, more preferably 50-65 ° C, even more preferably 55-65 ° C. ego; Preferably it is 150-350 bar, more preferably 150-300 bar, even more preferably 200-300 bar, even more preferably 220-280 bar. According to a specific embodiment of the present invention, the ginseng fat-soluble component supercritical extraction condition of the ginseng body is a temperature of 60 ℃ and 250 bar pressure.

On the other hand, the supercritical fluid extraction step, preferably under the conditions of 1-20 ml / min, more preferably 10-20 ml / min, even more preferably 13-18 ml / min of the flow rate of the supercritical fluid It can be carried out.

After extracting the ginseng fat-soluble component, it may be subjected to an additional filtration process to remove impurities contained in the extract before the application of step (c), the filter may be a centrifuge, a pressure filter or a diatomaceous earth filter.

Extraction method of the present invention may further use a co-solvent to further improve the extraction yield of ginseng fat-soluble components. For example, a supercritical fluid and a nonpolar solvent or a supercritical fluid and a polar solvent can be used. The nonpolar solvent includes any nonpolar solvent conventionally used in the art and is preferably selected from the group consisting of hexane, acetone, toluene, chloroform, dimethylsulfoxide (DMSO), tetrahydrofuran (THF), N, Dioxane, diethyl ether, N-methylpyrrolidone, benzene or mixtures thereof, preferably hexane. The polar solvent includes any polar solvent conventionally used in the art, and preferably alcohol, water, ethylene glycol, polyethylene glycol, propylene carbonate, formic acid, acetic acid, acetonitrile, chlorodifluoromethane Or a mixture thereof, more preferably an alcohol selected from the group consisting of methanol, ethanol, propanol, 1-hexanol, 2-methoxyethanol and combinations thereof, even more preferably methanol or ethanol Even more preferably ethanol. In the case of using a cosolvent, the flow rate may be 1-10 ml / min, preferably 1-5 ml / min, more preferably 1-3 ml / min.

Step (c): obtaining ginseng fat soluble component

Ginseng fat-soluble component is obtained from the extraction result of step (b).

The ginseng fat-soluble component extract of step (c) may be concentrated to a water content of 20% to 50%, and this concentrate may be prepared as a lyophilized powder. The concentration may be carried out through a variety of methods known in the art, for example, may be carried out according to the concentration under reduced pressure. The concentrate and lyophilized powder may be used for the production of the final product through an ultrahigh pressure sterilization process.

As used herein, the term “fat-soluble component” refers to a group of organic substances constituting a living body together with sugars, proteins, and the like, and includes lipids (eg, fatty acids, essential oils, phytosterols and organic acids) and phenolic compounds (polyacetylene and terpenoids). .

As used herein, the term “fatty acid” refers to a carboxylic acid including a long aliphatic chain, and includes both saturated and unsaturated fatty acids. The saturated fatty acid is a fatty acid that does not include a carbon-carbon double bond, and refers to a fatty acid having a general formula of CH ₃ (CH ₂ ) _n COOH (n is 1 to 34), and the unsaturated fatty acid is one or more in the molecule. Fatty acids comprising carbon-carbon double bonds and carboxyl groups, such as n-3 unsaturated fatty acids, n-6 unsaturated fatty acids, n-7 unsaturated fatty acids, n-9 unsaturated fatty acids, and other 2-pyridinepropanoic acids, 3-fur Nenmic acid, cyclopropylmethylphosphinic acid and cyclopropaneoctanoic acid.

According to a preferred embodiment of the invention, the fat soluble component is saturated fatty acid, n-3 unsaturated fatty acid, n-6 unsaturated fatty acid, n-7 unsaturated fatty acid or n-9 unsaturated fatty acid.

The n-3 unsaturated fatty acid may be hexadecatenoic acid, octadecatenoic acid, octadecatetraenoic acid, eicosataenoic acid, eicosateratenoic acid, eicosapentaenoic acid, henecosapentaenoic acid, Docosapentadenoic acid, docosahexaenoic acid, tetracosapentaenoic acid and tetracosahexaenoic acid, wherein the n-6 unsaturated fatty acids are octadecadienoic acid, octadecatenoic acid, eicosadienoic acid, Eicosatoenoic acid, eicosatetraenoic acid, docosadienoic acid, docosatetraenoic acid, docosapentaenoic acid, tetracosatetraenoic acid, tetracosapentaenoic acid and octadecatenoic acid, The n-7 unsaturated fatty acids include dodecenoic acid, tetradecenoic acid, hexadecenoic acid, decenoic acid, eicosenoic acid, docosenoic acid and tetracocenoic acid, and the n-9 unsaturated fatty acids are octadecenoic acid, eicosane Senoic acid, eicosatoenoic acid, docosenoic acid and tetracosenoic acid.

More preferably, the saturated fatty acid is docosanoic acid, eicosanoic acid, henecosanoic acid, dodecanoic acid, heptadecanoic acid, hexadecanoic acid, octadecanoic acid, octanoic acid, pentadecanoic acid, tetradecanoic acid, tri Cosanoic acid, nonadecanoic acid, hexanoic acid, decanoic acid, propanoic acid, undecanoic acid and 11,14-eicosanoic acid, the unsaturated fatty acids being 11-eicosenoic acid, 11,13-eicosadienoic acid, 13- Dococenoic acid, 9,12,15-octadecaterynoic acid, 9,12-octadecadienoic acid, 9-hexadecenoic acid, 9-octadecenoic acid, 11-octadecenoic acid, 8,11-octadecadier Nosanoic acid, 7,10-hexadecadienoic acid, 13-octadecenoic acid, 6,9,12-octadecatenoic acid, 9,15-octadecadienoic acid, eicosenoic acid, 10-undecenoic acid, 7- Hexadecenoic acid, 8,10-octadecenoic acid, 10,13-octadecadienoic acid and 9-dodecenoic acid.

Even more preferably the saturated fatty acid is docosanoic acid, eicosanoic acid, henecosanoic acid, dodecanoic acid, heptadecanoic acid, hexadecanoic acid, octadecanoic acid, octanoic acid, pentadecanoic acid, tetradecanoic acid and tri Cosanoic acid, the unsaturated fatty acid is 11-eicosenoic acid, 11,13-eicosadienoic acid, 13-dococenoic acid, 9,12,15-octadecatenoic acid, 9,12-octadecadienoic acid, 9-hexadecenoic acid and 9-octadecenoic acid.

As used herein, the term “organic acid” refers to an organic compound (eg, amino acid) having acidic properties, and the term “polyacetylene” is an acetylene hydrocarbon, a polyhydric unsaturated alcohol, and the term “terpenoid” It is an organic compound whose carbon number is a multiple of 5.

According to another aspect of the present invention, the present invention (a) as an active ingredient extract comprising a ginseng fat-soluble component obtained by the method of any one of claims 1 to 4; And (b) provides a pharmaceutical composition for preventing or treating cardiovascular diseases comprising a pharmaceutically acceptable carrier.

As used herein, the term “pharmaceutically effective amount” means an amount sufficient to achieve the efficacy or activity of the extract comprising the ginseng fat-soluble component described above.

According to a preferred embodiment of the present invention, the pharmaceutical composition may further include a blood circulation improving adjuvant.

The blood circulation improving adjuvant includes, for example, omega-3 fatty acid, omega-6 fatty acid and lecithin, and is preferably an omega-3 fatty acid. The omega-3 fatty acid includes EPA and DHA, and the omega-6 fatty acid includes gamma linolenic acid.

Cardiovascular diseases to which the pharmaceutical composition of the present invention may be applied include, but are not limited to, arterial obstruction, varicose veins, venous thrombosis, Burger's disease, lymphedema, peripheral vascular disease, hypertension, hyperlipidemia, angina pectoris, and stroke. .

When the composition of the present invention is used for the prevention or treatment of cardiovascular diseases, the composition of the present invention may be used alone, but may also be used in combination with other conventional chemotherapy or physical surgery therapy, and such combination therapy. If you can more effectively treat cardiovascular disease.

According to another aspect of the present invention, the present invention provides a pharmaceutical composition for improving blood circulation, comprising a ginseng fat-soluble component extracted by the method of the present invention.

The composition of the present invention exhibits an antithrombotic effect of reducing platelet aggregation by thrombin, an antithrombotic effect of decreasing prothrombin time, and an antithrombotic effect of increasing active partial thromboplastin time, and thus can be used as a pharmaceutical composition for smoothing blood circulation. have.

According to a preferred embodiment of the present invention, the pharmaceutical composition may further include a blood circulation improving adjuvant.

The blood circulation improving adjuvant includes, for example, omega-3 fatty acid, omega-6 fatty acid and lecithin, and is preferably an omega-3 fatty acid. The omega-3 fatty acid includes EPA and DHA, and the omega-6 fatty acid includes gamma linolenic acid.

When prepared with the pharmaceutical composition of the present invention, the pharmaceutical composition of the present invention comprises a pharmaceutically acceptable carrier. Pharmaceutically acceptable carriers included in the pharmaceutical compositions of the present invention are those commonly used in the preparation, such as lactose, dextrose, sucrose, sorbitol, mannitol, starch, acacia rubber, calcium phosphate, alginate, gelatin, Calcium silicate, microcrystalline cellulose, polyvinylpyrrolidone, cellulose, water, syrup, methyl cellulose, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate and mineral oil, and the like It doesn't happen. In addition to the above components, the pharmaceutical composition of the present invention may further include a lubricant, a humectant, a sweetener, a flavoring agent, an emulsifier, a suspending agent, a preservative, and the like. Suitable pharmaceutically acceptable carriers and formulations are described in detail in Remington ' s Pharmaceutical Sciences (19th ed., 1995).

The pharmaceutical composition of the present invention may be administered orally or parenterally, and in the case of parenteral administration, it may be administered by intravenous injection, subcutaneous injection, intramuscular injection, intraperitoneal injection, transdermal administration, or the like. Preferably the pharmaceutical composition of the present invention is administered orally.

The appropriate dosage of the pharmaceutical composition of the present invention may vary depending on factors such as the formulation method, administration method, age, body weight, sex, pathological condition, food, administration time, administration route, excretion rate, . The daily dosage of the pharmaceutical composition of the present invention is, for example, 0.0001-1000 mg / kg, preferably 1-10 mg / kg, based on ginseng fat-soluble component, more preferably 1-5 mg / kg.

The effective dosage range of the ginseng fat-soluble component contained in the composition may vary depending on various factors such as sex, severity, age, administration method, target cell, expression level, and can be easily determined by those skilled in the art. .

The pharmaceutical composition of the present invention may be formulated into a unit dose form by formulating it using a pharmaceutically acceptable carrier and / or excipient according to a method which can be easily carried out by a person having ordinary skill in the art to which the present invention belongs. Or by intrusion into a multi-dose container. The formulation may be in the form of solutions, suspensions, syrups or emulsions in oils or aqueous media, or in the form of extracts, powders, powders, granules, tablets or capsules, and may further comprise dispersants or stabilizers.

According to another aspect of the present invention, the present invention provides a food composition for improving blood circulation comprising a ginseng fat-soluble component extracted by the method of the present invention.

According to another aspect of the present invention, the present invention provides a food composition for improving blood circulation, comprising a ginseng fat-soluble component and a blood circulation improving aid.

The food composition for improving blood circulation of the present invention may include the ginseng fat-soluble component extracted by the method of the present invention, but is not limited thereto and may be based on other extraction methods (for example, hot water extraction or organic solvent extraction). It includes a food composition for improving blood circulation comprising a fat-soluble component as a main component.

The food composition of the present invention includes all forms such as functional foods, nutritional supplements, health foods and food additives. Food compositions of this type may be prepared in a variety of forms according to conventional methods known in the art. For example, as a health food, extracts of ginseng may be prepared in the form of tea, juice, and drink for drinking, granulated, encapsulated, and powdered and consumed. Powders, capsules, soft capsules, tablets, gums and adhesives It may be prepared and consumed in the form of a type liquid composition. Functional foods include beverages (including alcoholic beverages), fruits and processed foods (e.g. canned fruit, canned foods, jams, marmalade, etc.), fish, meat and processed foods (e.g. ham, sausage corned beef, etc.). ), Breads and noodles (e.g. udon, soba, ramen, spaghetti, macaroni, etc.), fruit juices, various drinks, cookies, syrups, dairy products (e.g. butter, cheese, etc.), edible vegetable oils, margarine, vegetable protein, retort It can be prepared by adding the ginseng extract of the present invention to foods, frozen foods and various seasonings (eg, miso, soy sauce, sauce, etc.).

In addition to the above-mentioned main components, the food composition of the present invention contains auxiliary components (vitamins B, C, E, beta-carotene, etc.), mineral-containing compounds (Ca, Mg, Zn, etc.), phospholipids (such as lecithin), maltol, oligosaccharides, amino acids, and the like. Among them, it is more preferable because a mixture of two to three components can be used, since the biological activity effect can be enhanced.

According to a preferred embodiment of the present invention, the food composition may further include a blood circulation improving adjuvant. The blood circulation improving adjuvant includes, for example, omega-3 fatty acid, omega-6 fatty acid and lecithin.

In addition to the above components, in order to enhance the taste as a known additive, natural flavors such as plum, lemon, pineapple or herb, or natural fruit juice, natural pigments such as chlorophyllin and flavonoid, and fructose, sweet components Honey, sugar alcohol, sugar and acidulants citric acid, sodium citrate can be used in combination.

In addition to the food composition of the present invention, various nutritional agents, vitamins, electrolytes, flavoring agents, coloring agents, pectic acid and salts thereof, alginic acid and salts thereof, organic acids, protective colloidal thickeners, pH adjusting agents, stabilizers, preservatives, glycerin, alcohols And carbonation agents used in carbonated beverages. In addition, the food composition of the present invention may contain flesh for preparing natural fruit juice, fruit juice beverage and vegetable beverage. These components may be used independently or in combination. The proportion of such additives is not critical but is usually selected in the range of 0.01 to 0.1 parts by weight per 100 parts by weight of the composition of the present invention.

The features and advantages of the present invention are summarized as follows:

(a) The present invention provides a method for extracting ginseng fat soluble components under conditions of a specific temperature and pressure, and a pharmaceutical composition or food composition comprising the ginseng fat soluble component extracted by the method.

(b) The method of the present invention can extract a fat-soluble component of various active ingredients of ginseng using a supercritical fluid extraction method in a high yield, it is possible to mass-produce a product using the ginseng fat-soluble component.

(c) The ginseng fat-soluble component according to the method of the present invention contains a large amount of various unsaturated fatty acids and saturated fatty acids, and is excellent in blood circulation improving efficacy.

(d) When the ginseng fat-soluble component is ingested with a blood circulation improving adjuvant, triglyceride and LDL-cholesterol levels are reduced and HDL-cholesterol levels are increased, thereby improving blood circulation improving efficacy, and thus various blood circulation improving pharmaceutical compositions Or as a food composition.

Figure 1 is an in vitro experiment of omega-3 and ginseng fat-soluble extract, and graphically shows the results of PT and APTT analysis. Normal: normal group (no treatment); Control: control (omega-3 10 mg / ml); 1-9: experimental group (10 mg / ml ginseng root fat-soluble component extract); 10-18: Experimental group (10 mg / ml ginseng body fat-soluble component extract).
Figure 2 is an in vitro experiment of omega-3, and ginseng fat-soluble extract + omega-3 mixed composition, it shows a graph of the PT and APTT analysis results. Normal: normal group (no treatment); Control: control (omega-3 10 mg / ml); 1-9: experimental group (10 mg / ml ginseng root fat-soluble component extract + 10 mg / ml omega-3); 10-18: Experimental group (10 mg / ml ginseng body fat-soluble component extract + 10 mg / ml omega-3).
Figure 3 is an in vivo experiment performed by dietary ingestion of ginseng fat-soluble component, it shows a graph of the results of PT and APTT analysis. Normal: normal group (no treatment); Control: control (HFD 45% high fat diet); Group 1: HFD 45% + omega-3; Group 2: HFD 45% + extract A (ginseng root); Group 3: HFD 45% + Extract B (Ginseng Body); Group 4: HFD 45% + omega-3 + extract A (ginseng root); Group 5: HFD 45% + omega-3 + extract B (ginseng body) (p <0.1).
Figure 4 is an in vivo experiment performed by orally administering ginseng fat-soluble component, it shows a graph of the PT and APTT analysis results. Normal: normal group (no treatment); Control: control (HFD 45% high fat diet); Group 1: HFD 45% + omega-3; Group 2: HFD 45% + extract A (ginseng root); Group 3: HFD 45% + Extract B (Ginseng Body); Group 4: HFD 45% + omega-3 + extract A (ginseng root); Group 5: HFD 45% + omega-3 + extract B (ginseng body) (p <0.1).
FIG. 5 is an in vivo experiment conducted by orally administering ginseng fat-soluble components, and shows the results of numerical analysis of blood triglycerides (FIG. 5A), HDL-cholesterol (FIG. 5B) and LDL-cholesterol (FIG. 5C). Group 1: normal group (no treatment); Group 2: control (HFD 45% high fat diet); Group 3: HFD 45% + omega-3; Group 4: HFD 45% + extract A (ginseng root); Group 5: HFD 45% + extract B (ginseng body); Group 6: HFD 45% + omega-3 + extract A (ginseng root); Group 7: HFD 45% + omega-3 + extract B (ginseng body) (p <0.1).

Hereinafter, the present invention will be described in more detail with reference to Examples. It is to be understood by those skilled in the art that these embodiments are only for describing the present invention in more detail and that the scope of the present invention is not limited by these embodiments in accordance with the gist of the present invention .

Example

Throughout this specification, "%" used to denote the concentration of a particular substance is intended to include solids / solids (wt / wt), solid / liquid (wt / The liquid / liquid is (vol / vol)%.

Example 1 Extract of Ginseng Fat-Soluble Ingredients

After washing lightly 6 years ginseng purchased at the market with hot water followed by shattering of the ginseng body and root, it was carried out to extract the ginseng fat-soluble components with a carbon dioxide supercritical extraction (supercritical fluid extraction devices: CO ₂ Supercritical Fluid Extraction, refurbishment Autoclave). Supercritical CO ₂ was used as an extraction solvent, and after use, the total amount was recovered by reducing to gaseous state.

Two kinds of ginseng samples, body and root, were used, and after crushing, the fat-soluble substance was extracted in the inlet of the supercritical fluid extractor to maximize the contact area with the solvent. The ginseng root part yielded 1.49% at 350 bar pressure and 40 ° C. and 1.30% at 250 bar and 60 ° C., yielding higher yield than other conditions. The ginseng body part showed a yield of 0.86% at a pressure of 250 bar and a temperature of 60 ° C. and a yield of 0.80% at 350 bar and a temperature of 50 ° C., showing a relatively high yield among other conditions. Among the root and body extracts of ginseng obtained using supercritical fluid extraction, the above four materials with high yield were selected as candidates. The extraction yield for each sample and condition using the above supercritical fluid extraction method is summarized in Table 1 and Table 2. As a result, it was confirmed that the ginseng root portion yielded relatively higher than the ginseng body portion, and the yield of extraction of fat-soluble components of the ginseng root and ginseng body was about 1%, thereby establishing the basic conditions for mass production.

Sample / Condition Extraction Yield Using Supercritical Fluid Extraction Method (Ginseng Root) number Temperature (℃) Pressure (bar) Time (min) Extraction yield (%) One 30 100 120 0.80 2 150 120 0.82 3 200 120 0.81 4 250 120 0.84 5 300 120 0.83 6 350 120 0.85 7 40 100 120 0.90 8 150 120 0.95 9 200 120 0.92 10 250 120 0.96 11 300 120 1.00 12 350 120 1.49 13 50 100 120 0.98 14 150 120 1.16 15 200 120 1.12 16 250 120 1.13 17 300 120 1.14 18 350 120 1.15 19 60 100 120 1.00 20 150 120 1.02 21 200 120 1.08 22 250 120 1.30 23 300 120 1.25 24 350 120 1.24

Sample / Condition Extraction Yield Using Supercritical Fluid Extraction Method (Ginseng Body) number Temperature (℃) Pressure (bar) Time (min) Extraction yield (%) 25 30 100 120 0.50 26 150 120 0.57 27 200 120 0.54 28 250 120 0.55 29 300 120 0.56 30 350 120 0.52 31 40 100 120 0.60 32 150 120 0.62 33 200 120 0.66 34 250 120 0.73 35 300 120 0.73 36 350 120 0.77 37 50 100 120 0.70 38 150 120 0.73 39 200 120 0.75 40 250 120 0.79 41 300 120 0.79 42 350 120 0.80 43 60 100 120 0.71 44 150 120 0.73 45 200 120 0.75 46 250 120 0.86 47 300 120 0.78 48 350 120 0.65

Example  2: Establishment of ingredient analysis condition of ginseng fat-soluble extract

The fat-soluble component analysis conditions were established using a gas chromatography-mass spectrometer (GC / MS Varian 4000, USA) to determine what components the ginseng extract of Example 1 is composed of. Extracts with relatively high yields were selected and analyzed for efficient analysis.

First Selection of Ginseng Extract number sample Temperature (℃) Pressure (bar) Time (min) One Ginseng root 40 150 120 2 40 250 120 3 40 350 120 4 50 150 120 5 50 250 120 6 50 350 120 7 60 150 120 8 60 250 120 9 60 350 120 10 Ginseng body 40 150 120 11 40 250 120 12 40 350 120 13 50 150 120 14 50 250 120 15 50 350 120 16 60 150 120 17 60 250 120 18 60 350 120

GC / MS measurement conditions of ginseng oil are as follows:

Analyzer GC / MS Varian 4000; Injection mode GC SPME; Syringe SPME syringe; Stirrer temperature 50 ° C., 30 minutes; Column VF5 MS; Injector temperature 200 ° C .; Detector temperature 200 ° C; Mass range 1000 Daltons; 86 minutes uptime.

Example  3: Supercritical  Analysis of Ginseng Extract Using Carbon Dioxide

The ginseng root extract obtained from the ginseng root and the body of Example 1 was analyzed, and the results are shown in Tables 4 and 5 below. The extraction conditions of Table 4 are 100-350 bar and 30-60 ° C, and the extraction conditions of Table 5 are 100-350 bar and 30-60 ° C.

As a result, a total of 30 components were detected in the ginseng root portion, a total of 36 components were detected in the ginseng body portion, and more components were detected in the ginseng body than the ginseng root which had a high extraction yield in Example 1.

Analysis of Ginseng Root Extract Using Supercritical Carbon Dioxide number fatty acid One 2 3 4 5 6 7 8 9 One 11-Eikosenosan o o o o o o o 2 11,13-Eicosadienoic acid o o o o o o 3 13-dokocenosan o o o o o o o 4 9,12,15-octadecatenoic acid o o o o o o o o 5 9,12-octadecadienoic acid o o o o o o o o 6 9-hexadecenoic acid o o o o o o o o 7 9-octadecenoic acid o o o o o o o 8 Dokosano o o o o o o o o 9 Eicosanosan o o o o o o o o 10 Heneiko Sano o o o o 11 Dodecanosan o o o 12 Heptadecanoic acid o o o o o o o o 13 Hexadecanoic acid o o o o o o o o 14 Octadecanoic acid o o o o o o o o 15 Octanoic acid o o o o o o 16 Pentadecanoic acid o o o o o o o o 17 Tetradecanoic acid o o o o o o o 18 Tricosanosan o o o o o 19 Nonadecano o o o 20 11-octadecenoic acid o o 21 Hexanoic acid o o 22 8,11-octadecadienoic acid o 23 7,10-hexadecadienoic acid o o 24 Decanoic acid o o o 25 13-octadecenoic acid o 26 6,9,12-octadecatenoic acid 27 9,15-octadecadienoic acid o 28 Eicosenoic acid o 29 Propanoic acid o 30 10-Undesenosan 31 2-pyridinepropanoic acid 32 7-hexadecenoic acid 33 Undecano 34 8,10-octadecenoic acid 35 11,14-Eicosanosan 36 10,13-octadecadiynoic acid 37 3-nonenoic acid 38 9-dodecenoic acid 39 Suishi Nanshan 40 Cyclopropylmethylphosphinic acid o o 41 Cyclopropaneoctanoic acid o o o

Analysis of Ginseng Body Extract Using Supercritical Carbon Dioxide number fatty acid 10 11 12 13 14 15 16 17 18 One 11-Eikosenosan o o o o o o o o 2 11,13-Eicosadienoic acid o o o o o o 3 13-dokocenosan o o o o o o o 4 9,12,15-octadecatenoic acid o o o o o o o o o 5 9,12-octadecadienoic acid o o o o o o 6 9-hexadecenoic acid o o o o o o o o o 7 9-octadecenoic acid o o o o o o o o 8 Dokosano o o o o o o o o 9 Eicosanosan o o o o o o o o 10 Heneiko Sano o o o o 11 Dodecanosan o o o o o 12 Heptadecanoic acid o o o o o 13 Hexadecanoic acid o o o o o 14 Octadecanoic acid o o o o o o o o o 15 Octanoic acid o o o o o o o o o 16 Pentadecanoic acid o o o o o o o 17 Tetradecanoic acid o o o o o o o 18 Tricosanosan o o o o o o 19 Nonadecano o o 20 11-octadecenoic acid o o 21 Hexanoic acid 22 8,11-octadecadienoic acid o o 23 7,10-hexadecadienoic acid o o 24 Decanoic acid 25 13-octadecenoic acid o 26 6,9,12-octadecatenoic acid o 27 9,15-octadecadienoic acid 28 Eicosenoic acid 29 Propanoic acid o o 30 10-Undesenosan o 31 2-pyridinepropanoic acid o 32 7-hexadecenoic acid o 33 Undecano o o 34 8,10-octadecenoic acid o 35 11,14-Eicosanosan o 36 10,13-octadecadiynoic acid o o o o 37 3-nonenoic acid o 38 9-dodecenoic acid o 39 Suishi Nanshan o 40 Cyclopropylmethylphosphinic acid 41 Cyclopropaneoctanoic acid o o o o o o

Example  4: improvement of blood circulation of ginseng fat-soluble component

4-1. Evaluation of blood circulation improving efficacy of omega-3

An experiment was performed to evaluate the effect of improving blood circulation using fat-soluble component extract or omega-3 extracted from the ginseng root and ginseng body.

After 7 weeks-old rats (Sprague Dauri, Samtako Co., Ltd.), the whole blood was treated and treated at 37 ° C. for 2 hours. Normal group was not treated with both ginseng fat soluble extract and omega-3, control group was treated with omega-3, experimental group was treated with ginseng fat soluble extract. Table 6 below is the ginseng soluble component extract extracted under the conditions of Table 3 of Example 2 No. 1-9 is a ginseng root fat soluble extract experimental group, No. 10-18 represents a ginseng body fat soluble extract experimental group. Blood coagulation tests, Prothrombin Time (PT) and Activated Partial Thromboplastin Time (APTT), were measured in each blood sample. will be.

In Vitro PT and APTT Assay division Concentration (mg / ml) PT (seconds) APTT (seconds) - Normal No treatment 10.05 ± 0.25 25.80 ± 0.20 - Control group Omega-3
10 mg / ml 10.85 ± 0.95 38.25 ± 0.35 One Experimental group
Ginseng Root Extract
10 mg / ml 9.10 ± 0.10 21.65 ± 0.35 2 9.45 ± 0.45 30.50 ± 2.10 3 8.95 ± 0.05 66.30 ± 2.00 4 9.60 ± 0.10 42.85 ± 1.15 5 8.25 ± 0.75 52.30 ± 2.10 6 9.50 ± 0.10 32.50 ± 0.10 7 9.30 ± 0.10 43.50 ± 3.10 8 8.95 ± 0.25 39.90 ± 1.3 9 9.80 ± 0.10 32.70 ± 0.10 10 Ginseng Body Extract
10 mg / ml 9.70 ± 0.11 52.80 ± 0.10 11 11.35 ± 1.15 42.55 ± 2.10 12 9.80 ± 0.10 44.20 ± 0.12 13 12.95 ± 1.25 83.40 ± 3.00 14 12.60 ± 0.10 72.90 ± 0.10 15 11.80 ± 0.30 29.55 ± 7.35 16 10.70 ± 0.20 35.30 ± 15.8 17 11.60 ± 0.10 16.30 ± 0.11 18 12.20 ± 0.90 14.60 ± 0.90

+: Shows statistical significance compared to control results (+: p <0.1).

As can be seen in Table 6 and Figure 1, there was no difference between the PT of all the experimental group and the control group (or normal group). APTT is 66.30 ± 2.00, 42.85 ± 1.15, 52.80 ± 0.10, 43.50 ± 3.10, 52.80 ± 0.10, 42.55 ± 2.10, 44.20 ± 0.12 in numbers 3, 4, 5, 7, 10, 11, 12, 13 and 14, respectively , 83.40 ± 3.00 and 72.90 ± 0.10, showing significant significance compared to 25.80 ± 0.20 of normal group and 38.25 ± 0.35 of control group. This indicates that ginseng fat-soluble component did not affect PT coagulation involving blood coagulation exogenous factor, but inhibited coagulation in APTT test involving endogenous factor.

In particular, APTT levels of ginseng body fat soluble component extracts were significantly higher than ginseng root fat soluble component extracts. The present inventors expected that ginseng root extract with higher extraction yield and containing more ingredients would show significant efficacy in evaluating in vitro hematopoietic improvement, but in ginseng body parts having relatively lower yield than ginseng root part with high yield. Blood circulation improvement effect was higher.

4-2. Evaluation of Blood Circulation Efficacy of Ginseng Fat-Soluble Extract and Omega-3 Mixtures

After collecting blood from 7-week-old rats, the following treatment was performed for 2 hours at 37 ° C. Normal group 1 was not treated with both ginseng fat-soluble extract and omega-3, the control group was treated only omega-3. Table 7 below is the ginseng soluble component extract extracted under the conditions of Table 3 of Example 2, No. 1 is a normal group, No. 2 is a control group, No. 3-18 is a ginseng fat-soluble extract and an experimental group of omega-3. Blood coagulation tests, Prothrombin Time (PT) and Activated Partial Thromboplastin Time (APTT), were measured in each blood sample.The values in Table 7 represent the mean ± standard error. will be.

In Vitro PT and APTT Assay division Concentration (mg / ml) PT (seconds) APTT (seconds) - Normal No treatment 15.40 ± 0.30 29.40 ± 0.70 - Control group Omega-3
10 mg / ml 16.30 ± 0.20 30.70 ± 2.05 One Experimental group
Ginseng Root Extract 10 mg / ml
+ Omega-3
10 mg / ml 11.60 ± 0.10 26.05 ± 1.00 2 11.50 ± 0.60 15.60 ± 0.85 3 11.80 ± 0.10 19.03 ± 0.11 4 11.50 ± 0.10 21.50 ± 2.10 5 12.60 ± 1.10 18.60 ± 2.30 6 11.60 ± 0.50 21.10 ± 1.60 7 9.20 ± 1.90 19.10 ± 0.60 8 11.40 ± 0.30 21.90 ± 0.20 9 13.50 ± 0.10 39.95 ± 2.35 10 Ginseng Body Extract
10 mg / ml
+ Omega-3
10 mg / ml 12.8 ± 0.10 44.40 ± 1.70 11 10.35 ± 3.15 27.03 ± 1 .00 12 12.7 ± 0.60 73.70 ± 3.40 13 12.80 ± 0.10 27.25 ± 1.60 14 12.25 ± 0.35 74.00 ± 1.60 15 13.65 ± 0.55 27.50 ± 1.80 16 13.15 ± 0.05 27.62 ± 3.00 17 13.15 ± 0.05 38.40 ± 0.30 18 12.40 ± 0.70 30.85 ± 13.55

+: Shows statistical significance compared to control results (+: p <0.1).

As shown in Table 7 and Figure 2, there was no difference between the PT of all the experimental group and the control group. APTT was 39.95 ± 2.35, 44.40 ± 1.70, 73.70 ± 3.40, 74.00 ± 1.60 and 38.40 ± 0.30 in Experiment Nos. 9, 10, 12, 14 and 17, respectively, against 29.40 ± 0.70 in the normal group and 30.70 ± 2.05 in the control group. Significance was shown.

This is again confirmed by the fact that ginseng fat-soluble component does not affect the PT test involving the coagulation exogenous factor, but shows the possibility of inhibiting coagulation in the APTT test involving the endogenous factor. In addition, it was confirmed that the blood coagulation inhibitory activity was increased compared to Example 4-1 when treated with a mixture of omega-3 and ginseng fat-soluble components.

Example  5. In the rat  Ginseng fat soluble Of extract of  Evaluation of blood circulation improvement efficacy

5-1. Of ginseng fat-soluble extract Dietary composition  Evaluation of blood circulation improvement efficacy through ingestion

Two ginseng fat soluble extracts (named ginseng soluble extracts No. 3 and 14 in Table 6, hereinafter referred to as extract A and extract B, respectively) selected based on the in vitro assay results were used in the in vivo experiment. Three-week-old rats (Sprague Dauri, Samtako Co., Ltd.) were bred for one week, followed by four to six weeks of dietary, high fat diet (HFD 45%) and fat-soluble components of ginseng. The blood circulation improving efficacy of the HFD 45% diet group was evaluated.

General Dietary and HFD 45% Dietary Composition Table Furtherance AIN-76A HFD 45% cal gm% kcal% gm% kcal% protein 20 21 24 20 carbohydrate 66 68 41 35 Fat 5 12 24 45 kcal / kg 3,902 - 4,776 - ingredient g kcal g kcal Casein (milk) 200 800 200 800 Corn starch 150 600 155.036 620 Sucrose 500 2,000 50 200 Dextrose 0 0 132 528 cellulose 50 0 50 0 Soybean oil 0 0 25 225 Lard 0 0 175 1,575 Mineral mixtures 35 0 35 0 Vitamin Mix 10 40 10 40 Tertiary Butylhydroquinone (TBHQ) 0 0 0.014 0 L-cysteine 0 0 3 12 Choline Hydrogen Tartrate 2 0 2.5 0 Sum 1,000 3,902 837.6 4,000

The design of the experimental group is as follows:

Omega-3 concentration 1.5 g / diet 1 kg; Ginseng oil concentration 100 mg / 1 kg diet; Group 1 general diet; Group 2 HFD 45%; Group 3: HFD 45% + omega-3; Group 4 HFD 45% + extract A; Group 5 HFD 45% + extract B; Group 6 HFD 45% + omega-3 + extract A; Group 7 HFD 45% + omega-3 + extract B.

After raising the rats with the dietary composition of Table 8, PT and APTT were measured and shown in Table 9. As a result, there was no significant PT value in all groups compared to group 2 (control), which received only 45% of HFD in SD rats (FIG. 3A). Ginseng fat-soluble component means that it does not affect blood coagulation exogenous factors. The APTT value was 29.2 ± 1.6 in group 2, 31.25 ± 0.3 in group 5, 34.55 ± 4.2 in group 6 and 36.4 ± 2.6 in group 7 (FIG. 3B).

PT and APTT Analysis Results of Dietary Intakes of Fat-Soluble Components group Mouse count PT (seconds) APTT (seconds) One Normal 3 9.3 ± 0.2 31.6 ± 1.5 2 HFD 45% 6 9.1 ± 0.1 29.2 ± 1.6 3 HFD 45% + ω-3 6 8.8 ± 0.1 28.35 ± 0.8 4 HFD 45% + A 6 8.5 ± 0.2 30.95 ± 1.2 5 HFD 45% + B 6 8.4 ± 0.3 31.25 ± 0.3 6 HFD 45% + A + ω-3 6 9.5 ± 0.5 34.55 ± 4.2 7 HFD 45% + B + ω-3 6 8.6 ± 0.1 36.4 ± 2.6

* (p <0.1)

The difference between the normal group (group 1) and the omega-3 (group 3) is expected to have an effect of 45% HFD, but there is some room for improved blood flow of omega-3. The APTT levels were higher in the group fed Omega-3 and Ginseng fat-soluble components than the group fed Omega-3 only, resulting in improved blood circulation.

5-2. Evaluation of Efficacy of Blood Circulation through Oral Administration of Ginseng Fat-Soluble Extracts PT  And APTT  analysis)

Compared to the case of Example 5-1, which evaluated the effect of improving blood circulation according to dietary intake, the effect was expected to be more excellent when the composition was orally administered, and the in vivo experiment was rerun with the following experimental design.

The design of the experimental group is as follows:

Omega-3 concentration 30 mg / day / rat; Ginseng oil concentration 2 mg / day / rat; Group 1 normal group; Group 2 control group HFD 45%; Group 3 HFD 45% + omega-3; Group 4 HFD 45% + extract A; Group 5 HFD 45% + extract B; Group 6 HFD 45% + omega-3 + extract A; Group 7 HFD 45% + omega-3 + extract B.

PT and APTT Analysis Results of Oral Administration of Fat-Soluble Components group Mouse count PT (seconds) APTT (seconds) One 5 10.60 ± 1.33 31.30 ± 0.77 2 10 8.57 ± 1.89 31.40 ± 0.97 3 10 9.19 ± 0.70 32.33 ± 1.42 4 10 9.23 ± 0.72 31.50 ± 1.58 5 10 9.44 ± 0.65 32.27 ± 0.78 6 10 9.28 ± 0.58 33.29 ± 0.77 7 10 9.34 ± 0.36 32.84 ± 1.07

* (p <0.1)

The figures in Table 10 represent the mean ± standard error. There was no change in PT values in all groups compared to group 2 (control), which received only 45% of HFD in SD rats (FIG. 4A), but APTT values were 32.27 ± 0.78 in group 5, 33.29 ± 0.77 in group 6, and group 7 Was 32.84 ± 1.07, which was significant compared to group 2 (control) 31.40 ± 0.97 (FIG. 4B). Group 6 showed the highest effect.

5-3. Evaluation of blood circulation improvement efficacy by oral administration of ginseng fat soluble extract (Neutral lipid and cholesterol level analysis)

In vivo experiments were performed to measure blood triglycerides, HDL-cholesterol and LDL-cholesterol levels in the same experimental design as in Example 5-2.

Triglyceride Analysis Results According to Oral Administration of Fat-Soluble Components division Group 1 Group 2 Group 3 Group 4 Group 5 Group 6 Group 7 density
(mg / dL) 152 250 210 207 215 138 178

As can be seen in Table 11 and FIG. 5A, the levels of triglycerides were significantly lower in all groups compared to the control group (group 2). In particular, compared to the control group, group 3 (omega 3 group) 16%, group 4 (extract A group) 17%, group 5 (extract B group) 14%, group 6 (omega 3 + extract A group) is about 45%, group 7 (omega 3 + extract B administration group) showed about 30% triglyceride reduction effect.

HDL-Cholesterol Analysis According to Oral Administration of Fat-Soluble Components division Group 1 Group 2 Group 3 Group 4 Group 5 Group 6 Group 7 density
(mg / dL) 29 22 35 35 29 36 37

As can be seen in Table 12 and FIG. 5B, the levels of HDL-cholesterol were 59% in group 3 (omega 3 group), 59% in group 4 (extract A group), 59%, group 5 (compared to control group G2). Extract B administration group) was increased by 32%, group 6 (omega 3 + extract A administration group) about 63%, group 7 (omega 3 + extract B administration group) about 68%. In particular, HDL levels were highest in Group 7 (omega 3 + extract B administration group) compared to other groups.

In addition, when comparing group 6 (omega 3 + extract A administration group) and group 4 (extract A administration group), both groups showed significantly higher HDL levels than the control group, but the synergistic effect with omega 3 was low. Comparing group 7 (omega 3 + extract B administration group) with group 5 (extract B administration group) showed a greater synergy effect with omega 3.

Analysis of LDL-Cholesterol According to Oral Administration of Fat-Soluble Components division Group 1 Group 2 Group 3 Group 4 Group 5 Group 6 Group 7 density
(mg / dL) 20 29 24 22 26 26 19

As can be seen in Table 13 and FIG. 5C, the LDL levels were lower in all groups than in the control group (group 2), and in particular, the level of LDL-cholesterol compared to the control group was 3 (omega 3 administration group). 17%, Group 4 (Extract A-administered group) 24%, Group 5 (Extract B-administered group) 10%, Group 6 (Omega 3 + Extract A-administered group) about 10%, Group 7 (Omega 3 + Extract B-administered group) Was reduced by about 34%. In addition, in the case of group 7, it was found that synergistic effects of omega 3 and sample B were significantly increased.

While the present invention has been particularly shown and described with reference to exemplary embodiments thereof, it is to be understood that the same is by way of illustration and example only and is not to be construed as limiting the scope of the present invention. Accordingly, the actual scope of the present invention will be defined by the appended claims and their equivalents.

Claims (19)

Extraction method of ginseng roots (seeds, 근 根) fat-soluble components comprising the following steps:
(a) placing ginseng roots in a supercritical extraction vessel;
(b) Ginseng roots and supercritical fluid carbon dioxide were contacted under conditions of a temperature of 40-60 ° C. and a pressure of 300-350 bar. (ⅰ) Docosanoic acid, eicosanoic acid, henecosanoic acid, dodecanoo Saturated fatty acids of acids, heptadecanoic acid, hexadecanoic acid, octadecanoic acid, octanoic acid, pentadecanoic acid, tetradecanoic acid, tricosanoic acid, nonadecanoic acid, hexanoic acid and decanoic acid, or (ii) 11-eicose Nosan, 11,13-eicosadienoic acid, 13-dococenoic acid, 9,12,15-octadecaterynoic acid, 9,12-octadecadienoic acid, 9-hexadecenoic acid, 9-octadecenoic acid, Extracting unsaturated fatty acids of 11-octadecenoic acid, 7,10-hexadecadienoic acid, and 6,9,12-octadecaterienoic acid; And
(c) obtaining the ginseng root fat-soluble component contained in the extraction result of step (b).
Extraction method of ginseng body fat-soluble components comprising the following steps:
(a) placing the ginseng body into a supercritical extraction vessel;
(b) Ginseng body and supercritical fluid carbon dioxide are contacted under the conditions of temperature 50-60 ℃ and pressure 250-350 bar. (iii) Docosanoic acid, eicosanoic acid, heeicosanoic acid, dodecano Saturated fatty acids of acids, heptadecanoic acid, hexadecanoic acid, octadecanoic acid, octanoic acid, pentadecanoic acid, tetradecanoic acid, tricosanoic acid, nonadecanoic acid, hexanoic acid, decanoic acid, propanoic acid and undecanoic acid, or (Ii) 11-eicosenoic acid, 11,13-eicosadienoic acid, 13-docosenoic acid, 9,12,15-octadecaterinoic acid, 9,12-octadecadienoic acid, 9-hexadecenoic acid , 9-octadecenoic acid, 11-octadecenoic acid, 8,11-octadecadienoic acid, 7,10-hexadecadienoic acid, 9,15-octadecadienoic acid, eicosenoic acid and 10,13-octadeca Extracting unsaturated fatty acid of diino acid; And
(c) obtaining ginseng body fat soluble components included in the extraction result of step (b).
delete A process according to claim 1 or 2, wherein step (b) is carried out under conditions of flow rate of 10-20 ml / min of supercritical fluid.
The method of claim 1 or 2, wherein the ginseng is selected from the group consisting of white ginseng, red ginseng, fresh ginseng, black ginseng, rice ginseng, raw ginseng, taegeuk ginseng, wild ginseng, camphor ginseng, and goat ginseng.
delete delete delete delete delete (a) an extract of ginseng soluble components obtained by the method of claim 1 or 2 as an active ingredient, and (b) at least one blood circulation improving agent selected from the group consisting of omega-3 fatty acids, omega-6 fatty acids and lecithin. As a pharmaceutical composition for improving blood circulation, wherein the ginseng fat-soluble component extract is a blood circulation improving pharmaceutical composition, characterized in that it inhibits endogenous (intrinsic) blood coagulation.
delete delete (a) an extract of ginseng fat-soluble components obtained by the method of claim 1 or 2, and (b) at least one blood circulation improving agent selected from the group consisting of omega-3 fatty acids, omega-6 fatty acids and lecithin. A food composition for improving blood circulation, wherein the ginseng fat-soluble component extract is a blood circulation improving food composition, characterized in that it inhibits intrinsic blood coagulation. delete delete delete delete delete

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