KR101237215B1 - Phamaceutical composition for prevention or treatment of enteritis - Google Patents

Abstract

The present invention is a composition for preventing or treating enteritis, because at least one of the pine pollen and acorn tree pollen, honey and propolis are mixed so that each of the pharmacological components are combined, effective in the prevention or treatment of enteritis, constitution Regardless, anyone can take it easily. Since it is manufactured using food and fermentation enzyme, no side effects occur in the human body, so it can be safely and easily taken.

Description

Pharmaceutical composition for prevention or treatment of enteritis

The present invention relates to a pharmaceutical composition for preventing or treating enteritis, and more particularly, to a pharmaceutical composition and a health functional food for enteritis prevention or treatment containing honey and propolis, at least one of pollen and acorn pollen. .

Enteritis refers to a disease in which the intestinal mucosa is damaged by food poisoning or bacterial infection of the intestinal mucosa. There are many causes of enteritis. Infectious enteritis caused by various viral infections such as food poisoning and bacterial infection is common, and there are also chronic diseases such as Crosin's disease and ulcerative colitis. The main symptoms are abdominal pain and diarrhea, sometimes accompanied by fever. In the case of abdominal pain, heavy pain, which cannot be accurately located, is most common and begins with such heavy pain and progresses to severely painful pain, such as diarrhea, ranging from as little as 2 to 3 to as many as 20 times. In case of various small intestine infections, mucus-like mucus does not come out in the stool, while the large intestine mucus is mixed with mucus.

Enteritis includes acute and chronic enteritis, which can be divided into infectious and non-infectious. Infectivity is caused by bacteria and viruses such as dysentery, enteritis Vibrio, Salmonella, and cholera. Non-infectiveness is caused by binge eating, binge drinking, food poisoning, and ingestion of fluorinated foods. Diarrhea, abdominal pain, vomiting, fever, etc. due to inflammatory changes in the gastrointestinal tract causes diarrhea due to diarrhea more than 10 times a day, causing systemic weakness. In some cases, it can quickly deteriorate and become very urgent. Especially in the elderly or children, enteritis can become very debilitating and severe in the few days. Chronic enteritis becomes chronic in acute enteritis, but sometimes it is chronic from the beginning. It occurs due to tuberculosis, parasites, ulcerative colitis, and the symptoms of acute enteritis continue, and the bowel movement is irregular and diarrhea and constipation are repeated. In addition, poor appetite, abdominal pain, bloating, malabsorption due to malnutrition and anemia is likely to occur. Warm and relax your stomach and combine your diet with medication.

In most cases of acute enteritis, it is usually better for 3-4 days without any special treatment. However, it sometimes progresses to chronic enteritis and may cause serious consequences in children and the elderly. It is good to receive. In general, chronic enteritis is stable, dehydration treatment by sap supplementation, and when infectious, antibiotics that are sensitive to each bacteria are administered. Vancomycin is administered for gastroenteritis, and corticosteroids are administered for radiation enteritis. In case of severe abdominal pain, antispasmodic can be used.

On the other hand, pine pollen refers to pollen that comes out of pine trees in spring. Unlike flowers using insects, pine trees, which are pollinated with wind, try to pollinate by making large amounts of pollen and blowing them in the wind. The pine pollen is composed of two large air bags when it is enlarged, and this structure allows it to fly well in the wind. The form is yellow, light green, and fine powder. Songhwa powder is famous for royal food, and in the past, Songhwa powder was used as a coating agent, and it was a drug corresponding to the current gel force or ampo gel M. Calendula powder is 7.6% moisture, protein 17.4%, fat 8.45%, carbohydrate 63.4%, mineral 3.2%, rich in protein, carbohydrates and minerals, and relatively high in vitamin C. Songhwa powder is good for preventing dementia because it expands blood vessels of the human body, and it is good for preventing dementia.It is effective in relieving the toxicity of oxygen compounds made by free radicals, diabetic and fatty liver, inhibiting oxidation reactions, inhibiting enteritis and skin beauty. .

Propolis is a compound made by mixing bees themselves saliva with resins collected from the skin and leaves of various plants to maintain their survival environment. It is a natural functional material having a function. Propolis contains more than 20 different flavonoids such as chrysine, apigenin, garranine, camphorol, quercetin, and the like, and these flavonoids are known to exhibit the functionality of propolis. In addition to flavonoids, trace elements include iron, zinc, potassium, manganese, copper, lead, and vitamins. Propolis has bactericidal action, has allergic disease, eczema, neurodermatitis, inflammatory effect on skin itch, analgesic action, pulmonary tuberculosis, bronchitis, bronchial asthma, gastric drainage, hepatitis, athlete's foot and allergic skin disease.

Honey (Apis cerana Fabricius) is a sweet liquid that honeybees carry plant honey and other sugars, process and store in honeycombs. Honey is the basic carbohydrate food for honey bees, which consists primarily of glucose and fructose and is the most biologically valuable nutrient. Honey is largely made through two processes of honey bee: biochemical maturation process that converts polysaccharides to monosaccharides by mixing enzymes with flower honey or honeydew, and physical maturation process that spreads and spreads over a large area of bees. Honey is antibacterial, hematopoietic, hemostatic, longevity, detoxification, painful, nutritional, wound astringent, seawater, ulcers, detoxification, asthma, symptoms of foreign body in the throat, chronic constipation, enteritis, roundworm It is effective in diseases, palliatives, erythema, urinary tract disease, thin wounds, nephritis, onset, incision, thin sores, gastric duodenal ulcers, black warts on the face, frostbite, ulcers and trauma and dermatitis.

Therefore, various foods using pineapple powder, honey and propolis having the above-described effects have been developed, and in particular, Korean Patent No. 367425 contains pineapple powder, fructose, glucose, maltitol, etc. Health food using Songhwa powder, which can be easily ingested, Korean Patent No. 838716 describes that Songhwa powder is effective in treating enteritis, and Korean Patent Publication No. 2010-98742 discloses desalted sea water, taurine, and vitamins. , Royal jelly and propolis, supplementary minerals such as D-sorbitol, polyhydric alcohols such as xylitol, honey, etc. The composition, Korean Laid-Open Patent No. 2003-35396 is prepared by mixing red ginseng powder, Baekbokyeong powder, honey, raw sap juice, The weak and poor digestion Chapter severe patients, seizures can often enteritis is started up, such as dietary supplements with ingredients that I gyeongokgo severe abdominal pain patients such effects.

However, as described above, a pharmaceutical composition for preventing or treating enteritis containing pine pollen or acorn pollen, honey, and propolis has not been developed.

Therefore, the present inventors have studied a pharmaceutical composition for preventing or treating enteritis, and the composition comprising at least one of pine pollen and acorn pollen, propolis and honey is excellent in the prevention and treatment of enteritis and proven to be stable. Since it is a food and herbal medicine, it was confirmed that it is effective in preventing and treating enteritis without side effects, and completed the present invention.

Therefore, the problem to be solved by the present invention is a mixture of at least one of pine pollen and acorn tree pollen, honey and propolis, excellent enteritis prevention or treatment effect, and enteritis prevention or treatment pharmaceutical composition and health functional food without side effects To provide.

Pharmaceutical composition for preventing or treating enteritis according to the present invention to solve the above problems comprises at least one 30-55 parts by weight, 30-55 parts by weight of honey, and 1-5 parts by weight of propolis in pine pollen and acorn tree pollen. .

Pharmaceutical composition for preventing or treating enteritis according to the present invention,

Mixing at least one of 30 to 55 parts by weight, 30 to 55 parts by weight of honey, and 1 to 5 parts by weight of propolis in one or more kinds of pine pollen and acorn plant (one step); And

Fermenting the mixture at 30-40 ° C. for 10-30 days (2 steps)

It includes.

When at least one of the pine pollen and acorn tree pollen, at least one content of the pine pollen and acorn tree pollen in less than 30 parts by weight in one step of mixing the honey and propolis, enteritis prevention or treatment effect may be insufficient, If it exceeds 55 parts by weight, the effect may be reduced. If the honey content is less than 30 parts by weight, enteritis prophylaxis or treatment effect may be insignificant, and when the honey content is more than 55 parts by weight may be less effective. If the propolis content is less than 1 part by weight, enteritis prophylaxis or treatment effect may be insignificant, and when it exceeds 5 parts by weight, the effect may be reduced.

When using a mixture of pine pollen and acorn tree pollen, it is preferable that the weight ratio of the acorn tree pollen to the pine pollen 1: 1: 0.5 ~ 2.

Fermenting the mixture mixed in the first step through the two steps of fermenting the mixture maximizing the pharmacological effect of pine pollen or acorn pollen, honey and propolis is excellent in preventing or treating enteritis. If the mixture fermentation temperature is less than 30 ℃ pharmacological effect may be similar to before the fermentation, if it exceeds 40 ℃ there may be no difference in pharmacological effect. If the fermentation period is less than 10 days, the pharmacological effect may be similar to before the fermentation, if more than 30 days there may be no difference in pharmacological effect.

The compositions according to the invention can be used in formulated oral dosage forms, respectively, in tablets, pills, powders, granules, capsules, suspensions, emulsions, syrups and the like according to conventional methods. Carriers, excipients and diluents that may be included in compositions comprising at least one of pollen and acorn pollen, honey and propolis include lactose, dextrose, sucrose, sorbitol, mannitol, xylitol, erythritol, maltitol, starch , Acacia rubber, alginate, gelatin, calcium phosphate, calcium silicate, cellulose, methyl cellulose, microcrystalline cellulose, polyvinyl pyrrolidone, water, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate and mineral oil Can be mentioned. When formulated, diluents or excipients such as fillers, weights, binders, wetting agents, disintegrating agents and surfactants are usually used. Solid preparations for oral administration include tablets, pills, powders, granules, capsules, and the like, and the solid preparations include at least one excipient such as starch, calcium carbonate, sucrose or lactose, gelatin, and the like. Mix is prepared. In addition to simple excipients, lubricants such as magnesium stearate and talc are also used. Oral liquid preparations include suspensions, solvents, emulsions, and syrups, and may include various excipients such as wetting agents, sweeteners, fragrances, and preservatives, in addition to commonly used simple diluents such as water and liquid parakines. have. The preferred dosage of the pharmaceutical composition of the present invention varies depending on the condition and the weight of the patient, the degree of disease, the drug form, the administration route and the period, but can be appropriately selected by those skilled in the art.

However, for the preferred effect, the pharmaceutical composition of the present invention is administered 5 to 100 g per day, preferably 10 to 50 g each. Administration may be administered once a day, may be divided several times, or may be taken before or after meals.

The dose is not intended to limit the scope of the invention in any way.

At least one of the pine pollen and acorn pollen of the present invention, honey and propolis mixed composition is a food and fermented food that can be used safely even for long-term use for prevention purposes because of no toxicity and side effects.

The health functional food of the present invention includes the form of tablets, capsules, pills, or liquids, and the food to which the pine pollen or acorn tree pollen, honey and propolis mixed composition of the present invention can be added, for example, Various foods, beverages, gums, teas, vitamin complexes, and health functional foods. In detail, the health function for enteritis prevention or treatment comprising at least one of the pine pollen and acorn pollen of the present invention, a composition containing a mixture of honey and propolis as an active ingredient, and a food acceptable additive Provide food.

The food supplement additive may be at least one selected from the group consisting of vitamins, amino acids, minerals, dietary fiber and at least one selected from the group consisting of acidulants, sweeteners, flavoring agents, natural juices. The vitamins may be at least one selected from the group consisting of vitamins A, B ₁ , B ₂ , B ₆ , B ₁₂ , C, D ₂ , D ₃ , E, K, and folic acid. The amino acids may be at least one selected from the group consisting of histidine, isoleucine, leucine, lysine, methionine, valine, cystine, tryptophan, threonine, phenylalanine and tyrosine. The minerals may be at least one selected from the group consisting of copper, magnesium, manganese, molybdenum, selenium, zinc, iodine, iron, potassium, calcium and chromium. The dietary fibers include cha cha blood, cellulose, hemicellulose, crystalline cellulose, lignin, pectin, alginic acid, polymanneuronic acid, guar gum, gum arabic, arabinogalactan, konjac mannan, inulin, levan, polydextrose, and indigestible maltodextrin. It may be at least one selected from the group containing. The acidulant may be at least one selected from the group containing citric acid, DL-peric acid and succinic acid. The sweetener may be at least one selected from the group consisting of xylitol, D-sorbitol, maltitol, mannitol, erythritol, lactose, glucose, fructose, sugar, aspartame, and stevioside. The flavoring agent may be at least one selected from the group consisting of mixed fruit flavor, apple flavor, yogurt flavor, banana flavor, apricot flavor, drink flavor, and lemon flavor. The natural juices may be one or more selected from the group consisting of apples, pears, pineapples, tangerines, peaches, guavas, apricots, strawberries, lemons, plums, and melons.

At least one of the pine pollen and acorn pollen according to the present invention, the composition containing honey and propolis may be used in combination with each of the ingredients that are effective in the prevention or treatment of enteritis can help in the treatment and prevention of enteritis and mixture The fermentation for a certain period of time can further maximize the pharmacological effect.

In addition, regardless of the constitution, anyone can easily take it, and since it is manufactured using food and fermentation enzymes, it may be safely and easily taken because no side effects occur in the human body.

1 is a process chart sequentially showing a manufacturing process for producing a pharmaceutical composition for preventing or treating enteritis according to the present invention.

Hereinafter, preferred embodiments of the present invention will be described. However, the present invention is not limited to the embodiments described herein and may be embodied in other forms.

Example 1 Preparation of a composition for preventing or treating enteritis

440 g of pine pollen, 440 g of honey, and 20 g of propolis were mixed and then fermented into a fermenter for 10 days to prepare a composition for preventing or treating enteritis.

<Example 2> Preparation for preventing or treating enteritis

In the same manner as in Example 1, 440 g of acorn tree pollen was added instead of pine pollen to prepare a composition for preventing or treating enteritis.

Example 3 Preparation of a composition for preventing or treating enteritis

In the same manner as in Example 1, 220g of pine pollen and 220g of acorn pollen were added to prepare a composition for preventing or treating enteritis.

<Examples 4-6> Preparation of enteritis prophylaxis or treatment

In the same manner as in Example 1, to prepare a composition for preventing or treating enteritis of Examples 4 to 6 under the same conditions as in Table 1.

<Comparative Examples 1 to 6> Comparative Composition Preparation

In the same manner as in Example 1, but the comparative compositions of Comparative Examples 1 to 6 were prepared under the same conditions as in Table 1. Comparative Example 6 was prepared as a composition without mixing the pine powder, honey and propolis after the fermentation process.

division Calendula Powder (g) Honey (g) Propolis (g) Example 1 440 440 20 Example 2 Acorn Flowerpot 440 440 20 Example 3 Calendula 220, Acorn Flowerpot 220 440 20 Example 4 300 550 50 Example 5 550 300 50 Comparative Example 1 200 680 20 Comparative Example 2 680 200 20 Comparative Example 3 - 850 50 Comparative Example 4 850 - 50 Comparative Example 5 850 50 - Comparative Example 6 440 440 20

<Test Example 1> Evaluation of enteritis improvement for acetic acid induction model

Enteritis improvement comparative evaluation of the composition prepared in Examples 1 to 5 and Comparative Examples 1 to 6 was performed.

The rats used in the experiment were sprague-dawley male rats (6 weeks old, body weight 180 ~ 200g), and were used after 7 days of adaptive breeding. The breeding temperature was 22 ± 2 ℃ and the lighting time was 12 hours (8: 00 ~ 20: 00).

After fasting the rats for 24 hours, the polyethylene tube (length 0.76 mm) of 8 cm length was pushed into the anus, and 7% acetic acid aqueous solution was slowly injected by 0.1 ml in a 1 ml dose to the rats. Colitis was induced. After 24 hours after the injection of acetic acid, except for the negative control, the composition prepared in Examples 1 to 5 and Comparative Examples 1 to 6 in each of the three mice to be tested once daily for 4 days in an amount of 100 mg / kg It was administered orally using a rat oral sonde.

Before the experiment, the body weights of the test subjects were measured. After 24 hours of the last oral administration, the weight change, the presence of diarrhea, the length and weight of the colon of each test subject were measured. The measurement results are shown in Tables 2 to 4 below.

1. Change in body weight (weight difference between rats before and after experiment)

division Weight change Example 1 2.74 ± 0.55 Example 2 2.76 ± 0.50 Example 3 2.75 ± 0.53 Example 4 2.81 ± 0.53 Example 5 2.71 ± 0.52 Comparative Example 1 1.61 ± 0.49 Comparative Example 2 1.65 ± 0.52 Comparative Example 3 -0.15 ± 0.51 Comparative Example 4 1.05 ± 0.48 Comparative Example 5 1.22 ± 0.47 Comparative Example 6 0.81 ± 0.46 Negative control group -2.31 ± 0.74 Normal 3.60 ± 0.58

As shown in Table 2, the composition of Examples 1 to 5 made of pine pollen or acorn tree pollen, honey and propolis is Comparative Example 3, containing no pine pollen or acorn tree pollen, Comparative Example 4 not containing honey And the weight gain was higher than that of the composition of Comparative Example 5 containing no propolis. In addition, the weight gain was found to be higher than that of the composition of Comparative Example 6 in which the pine powder, honey and propolis mixtures were not fermented.

Therefore, in Examples 1 to 5 according to the present invention, a composition prepared by mixing one or more of 30 to 55 parts by weight, 30 to 55 parts by weight of honey, and 1 to 5 parts by weight of propolis in pine pollen and acorn tree pollen is colitis. According to the weight loss inhibitory effect was found to be excellent.

2. The presence of diarrhea

Immediately before daily oral administration of the composition, each animal was checked for the occurrence of diarrhea.

division Diarrhea Example 1 Recovers from severe diarrhea and approaches normal stools Example 2 Recovers from severe diarrhea and approaches normal stools Example 3 Recovers from severe diarrhea and approaches normal stools Example 4 Recovers from severe diarrhea and approaches normal stools Example 5 Recovers from severe diarrhea and approaches normal stools Comparative Example 1 Close to stool in severe diarrhea Comparative Example 2 Close to stool in severe diarrhea Comparative Example 3 Close to stool in severe diarrhea Comparative Example 4 Close to stool in severe diarrhea Comparative Example 5 Close to stool in severe diarrhea Comparative Example 6 Close to stool in severe diarrhea Negative control group Diarrhea is severe, causing inflammation around the anus

The composition of Examples 1 to 5 prepared from pine pollen or acorn tree pollen, honey and propolis as shown in Table 3, Comparative Example 3, containing no pine pollen or acorn tree pollen, Comparative Example 4 and no honey Severe diarrhea recovery with colitis was higher than that of the composition of Comparative Example 5 containing no propolis. In addition, it was found that the severe diarrhea recovery rate caused by enteritis was higher than that of the composition of Comparative Example 6, which was not fermented with pine powder, honey and propolis mixture.

Therefore, in Examples 1 to 5 according to the present invention, a composition prepared by mixing one or more of 30 to 55 parts by weight, 30 to 55 parts by weight of honey, and 1 to 5 parts by weight of propolis in pine pollen and acorn tree pollen is colitis. Diarrhea inhibitory effect was found to be excellent.

3. length and weight of colon

24 hours after the oral administration of the final composition, each of the test subjects was degenerated from the cervical spine, and an autopsy was performed, and the colon was extracted. It was.

division Large intestine length (cm) Large intestine weight (kg) Example 1 13.71 ± 0.45 11.61 ± 0.53 Example 2 14.69 ± 0.43 11.67 ± 0.51 Example 3 14.70 ± 0.44 11.63 ± 0.52 Example 4 14.78 ± 0.95 11.78 ± 0.58 Example 5 13.68 ± 0.41 11.57 ± 0.51 Comparative Example 1 12.28 ± 0.47 11.19 ± 0.45 Comparative Example 2 12.46 ± 0.49 11.31 ± 0.57 Comparative Example 3 11.41 ± 0.43 9.19 ± 0.84 Comparative Example 4 11.46 ± 0.41 10.24 ± 0.47 Comparative Example 5 11.48 ± 0.52 10.35 ± 0.60 Comparative Example 6 11.35 ± 0.31 8.12 ± 0.47 Negative control group 11.19 ± 0.95 5.30 ± 0.35 Jeongsan-gun 15.59 ± 0.43 10.90 ± 0.20

The composition of Examples 1 to 5 prepared from pine pollen or acorn pollen, honey and propolis as shown in Table 4, Comparative Example 3, containing no pine pollen or acorn pollen, Comparative Example 4 and no honey The colorectal length and weight loss rate which appeared with colitis were lower than the composition of the comparative example 5 which does not contain propolis. In addition, it was shown that the length and weight loss rate of bowel appearing with enteritis were lower than those of the composition of Comparative Example 6 in which the pine powder, honey and propolis mixtures were not fermented.

Therefore, in Examples 1 to 5 according to the present invention, a composition prepared by mixing one or more of 30 to 55 parts by weight, 30 to 55 parts by weight of honey, and 1 to 5 parts by weight of propolis in pine pollen and acorn tree pollen is colitis. Inhibitory effect on colon length and weight reduction was excellent.

<Test Example 2> Evaluation of the effect of improving abdominal pain caused by enteritis

The comparative evaluation of the abdominal pain improvement effect appearing according to the enteritis of the composition prepared in Examples 1-5 and Comparative Examples 1-6.

The subjects of this study were 110 male and female patients in their 20s and 40s who complained of abdominal pain. The test subjects (110 persons) divided the composition prepared in Examples 1 to 5 and Comparative Examples 1 to 6 into groups of 11 each from August 1, 2010 to August 10, 2010 (10 days) A total of 45 g per day was taken at 8 am, 1 pm, and 7 pm, respectively, and all subjects maintained their existing lifestyle and eating habits during the experiment. The test results are shown in Table 5 below.

division

Improved abdominal pain following enteritis Number (persons) ratio(%) Example 1 9 90 Example 2 9 90 Example 3 10 100 Example 4 6 60 Example 5 7 70 Comparative Example 1 6 60 Comparative Example 2 7 70 Comparative Example 3 4 40 Comparative Example 4 5 50 Comparative Example 5 5 50 Comparative Example 6 3 30

As shown in Table 5, the composition of Examples 1 to 5 made of pine pollen or acorn tree pollen, honey and propolis, Comparative Example 3, which does not contain pine pollen, Comparative Example 4 and propolis that does not contain honey It was shown that the effect of improving abdominal pain caused by enteritis was higher than that of the composition of Comparative Example 5, which was not contained. In addition, the composition of Comparative Example 6 which was not fermented with pine powder, honey, and propolis mixture was found to have the lowest abdominal pain improvement effect caused by enteritis.

Therefore, the composition prepared by mixing at least one of 30 to 55 parts by weight, 30 to 55 parts by weight of honey, and 1 to 5 parts by weight of propolis in the pine pollen and acorn planter prepared in Examples 1 to 5 according to the present invention. It was confirmed that the effect of improving abdominal pain caused by enteritis was excellent.

Experimental Example 3 Evaluation of Diarrhea Improvement Effect According to Enteritis

The comparative evaluation of the diarrhea improvement effect appearing according to the enteritis of the composition prepared in Examples 1-5 and Comparative Examples 1-6.

The subjects were 110 males and females in their 20s and 40s who complained of diarrhea caused by enteritis. To the subjects (110 people), the composition prepared in Examples 1 to 5 and Comparative Examples 1 to 6 were divided into 11 groups from September 1, 2010 to September 10, 2010 (10 days). A total of 45 g per day was taken at 8 am, 1 pm, and 7 pm, respectively, and all subjects maintained their existing lifestyle and eating habits during the experiment. The test results are shown in Table 6 below.

division

Diarrhea due to enteritis Number (persons) ratio(%) Example 1 9 90 Example 2 9 90 Example 3 9 90 Example 4 10 100 Example 5 8 80 Comparative Example 1 5 50 Comparative Example 2 6 60 Comparative Example 3 4 40 Comparative Example 4 5 50 Comparative Example 5 4 40 Comparative Example 6 3 30

As shown in Table 6, the compositions of Examples 1 to 5 prepared from pine pollen or acorn pollen, honey and propolis, Comparative Example 3, which does not contain pine pollen, Comparative Example 4 and propolis that do not contain honey It was shown that the diarrhea improvement effect appeared with enteritis than the composition of the comparative example 5 which is not contained. In addition, the composition of Comparative Example 6, which was not fermented with pine pollen, honey and propolis mixture, was found to have the lowest diarrhea improvement effect caused by enteritis.

Therefore, the composition prepared by mixing at least one of 30 to 55 parts by weight, 30 to 55 parts by weight of honey, and 1 to 5 parts by weight of propolis in the pine pollen and acorn planter prepared in Examples 1 to 5 according to the present invention. It was confirmed that the diarrhea improvement effect appeared with enteritis.

<Test Example 4> Evaluation of the fever improving effect caused by enteritis

Comparative evaluation of the exothermic effect shown by the enteritis of the composition prepared in Examples 1-6 and Comparative Examples 1-6.

The subjects of this study were 120 male and female patients in their 20s and 40s who complained of fever symptoms. Test subjects (120 people) divided into 11 groups of 10 people each of the compositions prepared in Examples 1-6 and Comparative Examples 1-6 from October 1, 2010 to October 10, 2010 (10 days) A total of 45 g per day was taken at 8 am, 1 pm, and 7 pm, respectively, and all subjects maintained their existing lifestyle and eating habits during the experiment. The test results are shown in Table 7 below.

division

Improvement of fever following enteritis Number (persons) ratio(%) Example 1 9 90 Example 2 9 90 Example 3 9 90 Example 4 10 100 Example 5 8 80 Example 6 6 60 Comparative Example 1 6 60 Comparative Example 2 7 70 Comparative Example 3 3 30 Comparative Example 4 4 40 Comparative Example 5 5 50 Comparative Example 6 4 40

As shown in Table 7, the compositions of Examples 1 to 5 prepared from pine pollen or acorn pollen, honey, and propolis, Comparative Example 3, which does not contain pine pollen, Comparative Example 4, which does not contain honey, and propolis It was shown that the exothermic improvement effect which appeared by enteritis was superior to the composition of the comparative example 5 which is not contained. In addition, the composition of Comparative Example 6, which was not fermented with pine pollen, honey and propolis mixture, was found to have the lowest exothermic effect shown by enteritis.

Therefore, the composition prepared by mixing at least one of 30 to 55 parts by weight, 30 to 55 parts by weight of honey, and 1 to 5 parts by weight of propolis in the pine pollen and acorn planter prepared in Examples 1 to 5 according to the present invention. It was confirmed that the exothermic effect of silver enteritis was excellent.

Test Example 5 Acute Oral Administration of Rats

Acute toxicity experiments were performed on the pine pollen or acorn pollen, honey and propolis mixed compositions of Examples 1 and 2 of the present invention. 6-week-old specific hospital member (SPF) SD rats were used, and the experimental group was composed of 1 g / kg, 2 g / kg, and 5 g of the compositions of Examples 1 and 2 of the present invention, respectively, for each of 5 male and female animals. / Kg dose group, and the test material was suspended orally in 5% HPMC (hydroxypropylmethyl cellulose) solution. After administration of the test substance, mortality, clinical symptoms, and changes in body weight were observed, and hematological and blood biochemical tests were performed. Necropsy was performed to visually observe the abdominal and thoracic organ abnormalities.

As a result, no significant clinical symptoms or dead animals were noted in all animals treated with the test substance, and no toxic changes were observed in weight changes, blood tests, blood biochemical tests, and autopsy findings. In addition, as a result of comparing the microscopic findings of the experimental group and the control group not administered the composition of Examples 1 and 2 of the present invention, liver, spleen, kidney, brain, skin, reproductive system, gastrointestinal, colon, heart, lung, etc. It has been found that the composition of the present invention does not show toxicity in the parenchymal organs and the like. As a result, all the compositions of the present invention, which were tested, were determined to be a safe substance which does not show toxic changes in rats up to 5 g / kg.

The following describes a method for producing a drug and a functional food containing the composition of the present invention, the present invention is not intended to limit this, it is intended to explain in detail only.

Preparation Example 1 Preparation of Powder

100 mg of the composition of Example 1

Lactose 100mg

Talc 10mg

The above ingredients were mixed and filled in an airtight container to prepare powders.

Preparation Example 2 Tablet Preparation

100 mg of the composition of Example 1

Corn Starch 100mg

Lactose 100mg

2 mg magnesium stearate

After mixing the above components, tablets were prepared by tableting according to the usual preparation method of tablets.

Preparation Example 3 Preparation Example of Liquid

100 mg of the composition of Example 1

10g isomerized sugar

Mannitol 5g

Purified water

According to the conventional method of preparing a liquid solution, each component is added to the purified water to dissolve it, and then a suitable amount of lemon is added, the above components are mixed, adjusted to 100 ml by adding purified water, and then filled into a brown bottle to be sterilized to prepare a liquid solution. Was prepared.

Preparation Example 4 Health Food Manufacturing Example

500 mg of the composition of Example 1

Ferulic Acid Compound 500mg

Vitamin mixture proper amount

70 μg of vitamin A acetate

Vitamin E 1.0mg

Vitamin B1 0.13mg

Vitamin B2 0.15mg

Vitamin B6 0.5mg

0.2 µg of vitamin B12

Vitamin C 10mg

10 μg biotin

Nicotinamide 1.7mg

Folate 50 ㎍

Calcium Pantothenate 0.5mg

Mineral mixture

Ferrous Sulfate 1.75mg

Zinc Oxide 0.82mg

Magnesium carbonate 2.53mg

15 mg potassium monophosphate

Potassium Diphosphate 55mg

Potassium Citrate 90mg

100 mg potassium carbonate

Magnesium chloride 24.8mg

The vitamin and mineral mixtures are prepared by mixing the relatively suitable ingredients for health foods in a preferred embodiment, but the mixing ratio may be arbitrarily modified, and the above ingredients are mixed according to a general health food manufacturing method, and then granulated. It can be prepared and used in the manufacture of health food compositions according to conventional methods.

<Example 5> Healthy drink manufacturing example

1000 mg of the composition of Example 1

Citric acid 500mg

100g oligosaccharide

Plum concentrate 2g

1 g of taurine

Add 900 ml of purified water

After mixing the above components in accordance with a conventional healthy beverage manufacturing method, and stirred and heated at 85 for about 1 hour, the resulting solution is filtered and obtained in two sterilized containers, sealed sterilization and refrigerated and then stored in the present invention It was used to prepare a healthy beverage composition. Although the composition ratio is a mixture of the components suitable for the preferred beverage as a preferred embodiment, the blending ratio may be arbitrarily varied according to the regional and national preferences such as the demand level, the demanding country, and the intended use.

Claims (3)

30 to 55 parts by weight of at least one species of pollen and acorn tree pollen, 30 to 55 parts by weight of honey, and 1 to 5 parts by weight of propolis as an active ingredient, characterized in that fermented at 30 to 40 ℃ for 10 to 30 days Pharmaceutical composition for preventing or treating enteritis. It contains at least 30 to 55 parts by weight, 30 to 55 parts by weight of honey, and 1 to 5 parts by weight of propolis, and fermented at 30 to 40 ° C. for 10 to 30 days as an active ingredient. Functional food for preventing or improving enteritis, characterized in that. Mixing at least one of 30 to 55 parts by weight, 30 to 55 parts by weight of honey, and 1 to 5 parts by weight of propolis in one or more kinds of pine pollen and acorn plant (one step); And
Fermenting the mixture at 30-40 ° C. for 10-30 days (step 2);
Method for producing a pharmaceutical composition for preventing or treating enteritis, characterized in that it comprises a.

Images