CN104997816B - Improve the pine pollen composition and application thereof of gastrointestinal function - Google Patents
Improve the pine pollen composition and application thereof of gastrointestinal function Download PDFInfo
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Abstract
The present invention relates to nutrition, health food and field of medicine preparations.The purpose of the present invention is to provide the pine pollen compositions that a kind of effect more preferably improves gastrointestinal function.Broken masson pine pollen and the natural pollen pini of non-broken wall are specifically applied in combination by a certain percentage, then adds the dosage form that pharmaceutically acceptable auxiliary material or complementary ingredient are prepared.Broken pollen and natural pollen are applied in combination the present invention, have the function of synergistic, expansion using face.Broken masson pine pollen is more advantageous to the release and absorption of effective component, can improve utilization of the body to pollen pini nutriment, is conducive to effective component and is absorbed into blood rapidly, reaches lesion and plays pharmacodynamics effect.Natural pollen pini dietary fiber abundant can not only improve intestine microenvironment, moreover it is possible to promote digestion waste and the exclusion of other metabolic toxicities, be conducive to the recovery and improvement of gastrointestinal function.The combination of broken masson pine pollen and natural pollen pini would be even more beneficial to pollen pini and play the effect for adjusting gastrointestinal function.
Description
Technical field
The present invention relates to nutrition, health food and field of medicine preparations, more particularly to a kind of adjusting gastrointestinal function
Pine pollen composition and preparation method thereof.
Background technique
Gastrointestinal tract is important digestive organs, human body to maintain normal physiological biochemical function, immune function, growth and development and
The vital movements such as metabolism just must provide main energy sources by the food of pipe intestinal digesting.Gastronintestinal system passes through food
Intake, transhipment, digestion and absorption and the activity such as excretion of residue be human body power supply, in addition, gastronintestinal system still has part
Endocrine and immune function.The diversification of gastrointestinal system function determines that the imbalance of gastrointestinal function and decline will all cause one
The obvious clinical symptoms of series.Specifically main includes three aspects: (1) paralysis of stomach caused by gastrointestinal motility disorder and paralysis
Property intestinal obstruction etc.；(2) ulcer and inflammation etc. caused by normal mucosa defencive function is impaired；(3) malabsorption and dyssecretosis institute
Cause diarrhea, constipation etc..The normal of gastrointestinal function is of great significance for maintenance body other function, raw however as the modern times
The change of quickening, dietary structure that movable joint is played and the rising of competitive pressure, enterogastric diseases disease incidence just rises year by year at present.
The product position of most of recuperating gastrointestinal tract function is all more single currently on the market, such as the weak, intestines for WeiDongLi Capsule
The monotherapy such as road hypoperistalsis, flora imbalance, gastrointestinal ulceration, although there is certain curative effect, attends to one thing and lose sight of another, can not be entirely square
Position comprehensively regulating.For portioned product even there are also some serious toxic side effects, such as many relax bowel and defecation products all contain rheum officinale, reed
The more strong drug such as luxuriant growth, folium sennae can also cause some toxicitys to react while improving clinical symptoms, and using face is inevitable
It is subject to certain restrictions.
Pollen pini is the male sex-cell of pinaceae plant masson pine, Chinese pine or other congeners, is that pinaceae plant passes
Ancestor connects the substance in generation.Pollen pini protein rich in, 21 kinds of amino acid, carbohydrate (carbohydrate), lipid are (especially rich
Rich polyunsaturated fatty acid), nearly hundred kinds of organized enzymes, 10 multivitamins, more than 50 kinds of macro- and trace-elements, more than 10 kinds of flavones
And 200 plurality of active ingredients such as carrotene, nucleic acid, polysaccharide, phytosterol, auxin, and nutrition-allocated proportion balances, and it is contained
Nutriment it is comprehensive with balanced in nature there are no any wholefoods to match in excellence or beauty therewith.With in recent years to pine
Many pharmacological actions of pollen more in-depth study, pollen pini are found.According to current documents and materials, pollen pini, which mainly has, exempts from
Epidemic disease adjusting, antifatigue, antitumor, free radical resisting, regulating lipoid and reducing blood pressure adjust blood glucose, are liver protecting, resistance prostatitis, anti-radiation, beautiful
The effects of holding beauty treatment, recuperating gastrointestinal tract.Its gastrointestinal tract adjustment effect is especially prominent in the numerous pharmacological action of pollen, flower
Powder energy is comprehensive, multiple target point adjusts gastrointestinal function, so that gastrointestinal function is restored to normal level, is mainly manifested in following three
Aspect:
1) enhance gastroenteritic power, promote emptying: research shows that digestive ferment abundant can whet the appetite in pollen pini, being promoted
Level of serum gastrin enhances WeiDongLi Capsule, promotes gastric emptying, improves pipe intestinal digesting ability.Pollen pini can also stimulate enteron aisle
The movement of smooth muscle promotes intestines peristalsis, can significantly improve constipation symptom.
2) gastrointestinal mucosa is protected, enhance absorption of nutrient ingredients: experimental study shows that pollen pini can significantly improve gastrointestinal tract
Mucous membrane condition in damaged inhibits gastrointestinal tract inflammation.The flavones contained in pollen pini has significant anti-oxidative stress, can reduce
Radical reaction, to play the above effect, furthermore the substances such as amino acid abundant, vitamin also contribute to being damaged in pollen pini
The reparation and the gastrointestinal inflammatory development of inhibition of mucous membrane.
3) improve environment in stomach and intestine, reduce the accumulation of metabolic toxicities: the imbalance of gastrointestinal tract microenvironment may cause various aspects
Symptom, such as diarrhea, constipation, acne, halitosis.Pollen pini dietary fiber rich in such as pectic substance, lignin, fiber
Element can stimulate intestines peristalsis to promote defecation.Insoluble diedairy fiber can absorb large quantity of moisture, can increase defecation volume and excrement
Moisture content, these are all conducive to relief of constipation symptom, while the exclusion of stool alleviates the accumulation of organism metabolism toxin, can be into
The symptoms such as acne, halitosis caused by one step is alleviated because of gastrointestinal dysfunction.Experimental study also shows that pollen pini is able to maintain that enteron aisle
Colony balance can significantly improve the symptoms such as diarrhea caused by flora imbalance, indigestion.
Pollen pini is with its nutriment abundant and its comprehensive pharmacodynamics effect in terms of recuperating gastrointestinal tract function, for exploitation
Completely new gastrointestinal tract conditioning products provide direction.Preserved egg powder have thicker cell wall, this make wherein effective component it is molten
Out slowly, experiment in vitro research shows that in broken masson pine pollen the dissolution of effective component be significantly faster than that non-broken masson pine pollen.Therefore, mesh
It is raw material that preceding pollen pini product most of in the market, which all uses broken pollen, this is not have for the product for developing pollen other function
It is influential, or even play the role of some beneficial.
But the present inventor studies for a long period of time, the pollen pini of broken wall finds broken the opsonic action of gastrointestinal function
Wall pollen pini is not necessarily best in terms of recuperating gastrointestinal tract function.
Summary of the invention
The purpose of the present invention is to provide the pine pollen compositions that a kind of effect more preferably improves gastrointestinal function.Tool
Body is broken masson pine pollen and the natural pollen pini of non-broken wall to be applied in combination by a certain percentage as main active, then adds pharmacy
The preparation that upper acceptable auxiliary material or complementary ingredient are prepared.
Technical solution of the present invention: the raw material comprising following weight proportion is that the suitable clinic that active constituent is prepared makes
Preparation:
6-25 parts of broken masson pine pollen, natural pollen pini 10-30 parts.
The pharmaceutically acceptable complementary ingredient, it has certain physiological activity, but the addition of the ingredient will not change
Become the leading position of aforementioned pharmaceutical compositions in the course of disease treatment, and only play auxiliary effect, these auxiliary effects are only
It is only the utilization to the ingredient known activity, is the usual adjuvant treatment modality of field of medicaments.
The broken masson pine pollen is the pollen pini of 95% or more sporoderm-broken rate.
The natural pollen pini be masson pine, Chinese pine and other belong to one of pollen or a variety of.
As preferred embodiments of the present invention, it is raw material such as comprising lower weight proportion plus pharmaceutically acceptable auxiliary material
The preparation for the suitable clinical use being prepared:
10-20 parts of broken masson pine pollen, natural pollen pini 14-25 parts.
Further, it is that the raw material comprising following weight proportion is fitted plus what pharmaceutically acceptable auxiliary material was prepared
Close the preparation of clinical use:
12 parts of broken masson pine pollen, 16 parts of natural pollen pini.
Wherein the broken masson pine pollen be broken wall masson pine, Chinese pine and other belong to pollen through breaking-wall cell treated flower
It is one or more in powder.
The natural pollen pini be masson pine, Chinese pine and other belong to pure natural pollen of the pollen without any working process
One of or it is a variety of.
It is that the natural pollen pini is natural Pollen of Masson Pine as preferred embodiments of the present invention, is secondly Chinese pine pollen.
It is that the broken masson pine pollen is broken wall Pollen of Masson Pine as preferred embodiments of the present invention, is secondly Chinese pine pollen.
The present invention also provides the preparation methods of above-mentioned composition, it includes following operating procedure:
Broken masson pine pollen and natural pollen pini is taken to combine mixing according to the ratio, in addition pharmaceutically acceptable auxiliary material or complementary
Ingredient is prepared into pharmaceutically common formulations.
The present invention also provides above-mentioned pine pollen compositions in preparation improvement gastrointestinal function and because gastrointestinal function is lacked of proper care
Caused by purposes in related symptoms drug.
Further, the improvement gastrointestinal function refers to enhancing WeiDongLi Capsule, promotes gastric emptying, promote intestines peristalsis, protect
Protect gastrointestinal tract mucous, inhibition gastrointestinal ulceration, adjust micro ecology of gastrointestinal tract environment etc..
Further, related symptoms caused by improving because of gastrointestinal function imbalance refer to because of functional disturbances of gastrointestinal tract or decline
The manifest symptom of clinical observable caused by moving back.As because flatulence caused by gastric dynamic dysfunction, it is inverse vomit, sour regurgitation；Because gastrointestinal mucosa damages
Gastroenteritic ulcer, gastrointestinal bleeding etc. caused by wound；Acne, halitosis because caused by constipation accumulates internal metabolic toxicities, because of stomach
Diarrhea, abdominal pain, intestinal colic caused by intestine microenvironment is unbalance etc..
Further, the drug refer to enhancing WeiDongLi Capsule, promote gastric emptying, inhibit gastroenteritic ulcer, protection gastrointestinal mucosa,
Relax bowel and defecation, the drug for improving intestinal microecology.
Broken masson pine pollen and natural pollen pini are applied in combination the present invention, have the function of synergistic, expansion using face.
Broken masson pine pollen is more advantageous to the release and absorption of effective component, can improve utilization of the body to pollens nutrition substance, be conducive to
Effective component is absorbed into rapidly blood, reaches lesion and plays pharmacodynamics effect.Natural pollen pini dietary fiber abundant can not only
Improve intestine microenvironment, moreover it is possible to promote digestion waste and the exclusion of other metabolic toxicities, be conducive to the extensive of gastrointestinal function
Multiple and improvement.It is better that the improvement of intestine microenvironment and the timely removing of metabolic waste, toxin are all conducive to gastrointestinal tract
The function of digesting and assimilating is played, being absorbed and utilized for pollen pini nutriment is thus more advantageous to.Therefore, broken masson pine pollen and day
The effect that pollen pini plays adjusting gastrointestinal function that would be even more beneficial to is used in combination in right pollen pini.
Below by way of specific embodiment, the present invention is described in further detail, but is not intended to limit the present invention, ability
Field technique personnel are variously modified and replace according to the present invention, and as long as it does not depart from the spirit of the invention, should belong to the present invention
Scope of the appended claims.
Specific embodiment
The Pollen Assemblage object provided by the invention for improving gastrointestinal function includes that the raw material of following weight proportion is prepared
Suitable clinical use preparation:
6-25 parts of broken masson pine pollen, natural pollen pini 10-30 parts.
The broken masson pine pollen is natural pollen pini through conventional broken wall treatment, and the sporoderm-broken rate of pollen pini is made to reach 95% or more
Pollen pini.For example, being dried to moisture 6% hereinafter, then zero after natural pollen being rinsed with water, being impregnated 2-5 hours
Freeze rapidly under the conditions of lower 30 DEG C, is carried out broken wall treatment 10-20 minutes through low temperature concussion micronizer, broken wall can be obtained
The broken masson pine pollen of 95% or more rate.It is of course also possible to buy the broken masson pine pollen of commercially available 95% or more sporoderm-broken rate.
In addition, the present invention also can control the technology for broken wall of pollen pini, so that the sporoderm-broken rate of pollen pini is in range appropriate,
Reach the ratio of broken masson pine pollen and non-broken masson pine pollen in the scope of the present invention, achieves the object of the present invention.
The natural pollen pini be masson pine, Chinese pine and other belong to one of pollen or a variety of.
As preferred embodiments of the present invention, it is the preparation that the raw material comprising following weight proportion is prepared:
10-20 parts of broken masson pine pollen, natural pollen pini 14-25 parts.
Further, it is the preparation that the raw material comprising following weight proportion is prepared:
12 parts of broken masson pine pollen, 16 parts of natural pollen pini.
Wherein the broken masson pine pollen be masson pine, Chinese pine and other belong to pollen through in breaking-wall cell treated pollen
It is one or more.
The natural pollen pini be masson pine, Chinese pine and other belong to one of pollen or a variety of.
It is that the natural pollen pini is natural Pollen of Masson Pine as preferred embodiments of the present invention, is secondly Chinese pine pollen.
It is that the broken masson pine pollen is broken wall Pollen of Masson Pine as preferred embodiments of the present invention, is secondly Chinese pine pollen.
Pharmaceutically acceptable auxiliary material of the present invention includes but are not limited to filler (diluent), lubricant (helps stream
Agent or antitack agent), dispersing agent, wetting agent, adhesive, regulator, solubilizer, antioxidant, bacteriostatic agent, emulsifier, disintegrating agent
Deng.Adhesive include syrup, Arabic gum, gelatin, sorbierite, tragacanth, cellulose and its derivates (such as microcrystalline cellulose,
Sodium carboxymethylcellulose, ethyl cellulose or hypromellose etc.), gelatine size, syrup, starch slurry or polyvinylpyrrolidine
Ketone etc.；Filler includes lactose, Icing Sugar, dextrin, starch and its derivative, cellulose and its derivates, inorganic calcium salt (such as sulfuric acid
Calcium, calcium phosphate, calcium monohydrogen phosphate, precipitated calcium carbonate etc.), sorbierite or glycine etc.；Lubricant includes superfine silica gel powder, stearic acid
Magnesium, talcum powder, aluminium hydroxide, boric acid, hydrogenated vegetable oil, polyethylene glycol etc.；Disintegrating agent includes starch and its derivative (such as carboxylic
Methyl starch sodium, Explotab, pregelatinized starch, modified starch, hydroxypropul starch, cornstarch etc.), polyvinyl pyrrole
Alkanone or microcrystalline cellulose etc.；Wetting agent includes lauryl sodium sulfate, water or alcohol etc.；Antioxidant packages contain sodium sulfite, sulfurous
Sour hydrogen sodium, sodium pyrosulfite, dibutyl benzoic acid etc.；Bacteriostatic agent includes 0.5% phenol, 0.3% cresols, 0.5% anesin
Deng；Regulator includes hydrochloric acid, citric acid, potassium hydroxide (sodium), sodium citrate and buffer (including phosphoric acid dioxy sodium and phosphoric acid hydrogen
Disodium) etc.；Emulsifier includes Tween-80, does not have that sour sorb is smooth, pluronic gram F-68, lecithin, Fabaceous Lecithin etc.；Solubilising
Agent includes Tween-80, bile, glycerol etc..
The pharmaceutically acceptable complementary ingredient, it has certain physiological activity, but the addition of the ingredient will not change
Become the leading position of aforementioned pharmaceutical compositions in the course of disease treatment, and only play auxiliary effect, these auxiliary effects are only
It is only the utilization to the ingredient known activity, is the usual adjuvant treatment modality of field of medicaments.Such as on the basis of the present invention
It adds with probiotics, the Bismuth Potassium Citrate with protection gastrointestinal mucosa effect, tripotassium for improving gastrointestinal bacterial flora structure
Two bismuth citrates etc. assist functional component.
Those skilled in the art are readily apparent that the active constituent of the present composition can also cooperate the treatment of current routine
The drugs such as gastroenteritic ulcer, constipation, diarrhea are used together.
Wherein, the dosage form is through gastrointestinal tract absorption type.For example, tablet, powder, pill, capsule, granule or mouth
Take liquid.
The present invention also provides above-mentioned pine pollen compositions in preparation improvement gastrointestinal function and because gastrointestinal function is lacked of proper care
Caused by purposes in related symptoms drug.
Further, the improvement gastrointestinal function refers to enhancing WeiDongLi Capsule, promotes gastric emptying, promote intestines peristalsis, protect
Protect gastrointestinal tract mucous, inhibition gastrointestinal ulceration, adjust micro ecology of gastrointestinal tract environment etc..
Further, related symptoms caused by improving because of gastrointestinal function imbalance refer to because of functional disturbances of gastrointestinal tract or decline
The manifest symptom of clinical observable caused by moving back.As because flatulence caused by gastric dynamic dysfunction, it is inverse vomit, sour regurgitation；Because gastrointestinal mucosa damages
Gastroenteritic ulcer, gastrointestinal bleeding etc. caused by wound；Acne, halitosis because caused by constipation accumulates internal metabolic toxicities, because of stomach
Diarrhea, abdominal pain, intestinal colic caused by intestine microenvironment is unbalance etc..
Further, the drug refer to enhancing WeiDongLi Capsule, promote gastric emptying, inhibit gastroenteritic ulcer, protection gastrointestinal mucosa,
Relax bowel and defecation, the drug for adjusting intestinal microecology.
The following are specific examples.
Embodiment 1
6 parts of broken masson pine pollen, 11 parts of natural pollen pini, the above raw material combine mixing according to the ratio, add microcrystalline cellulose, carboxylic
The pharmaceutically acceptable auxiliary material such as sodium carboxymethylcellulose pyce, magnesium stearate is prepared into tablet.
Embodiment 2
12 parts of broken masson pine pollen, 14 parts of natural pollen pini, combination mixes according to the ratio, in addition superfine silica gel powder, magnesium stearate etc.
Pharmaceutically acceptable auxiliary material is prepared into capsule.
Embodiment 3
8 parts of broken masson pine pollen, 12 parts of natural pollen pini, combination mixes according to the ratio, in addition pharmaceutically acceptable auxiliary material system
For at suspension type oral solution.
Embodiment 4
12 parts of broken masson pine pollen, 16 parts of natural pollen pini, combination mixes according to the ratio, in addition pharmaceutically acceptable auxiliary material system
For at granule.
Embodiment 5
10 parts of broken masson pine pollen, 18 parts of natural pollen pini, combination mixes according to the ratio, in addition pharmaceutically acceptable auxiliary material system
For at granule.
Embodiment 6
14 parts of broken masson pine pollen, 16 parts of natural pollen pini, combination mixes according to the ratio, in addition pharmaceutically acceptable auxiliary material system
For at granule.
Embodiment 7
6 parts of broken masson pine pollen, 30 parts of natural pollen pini, combination mixes according to the ratio, in addition pharmaceutically acceptable auxiliary material system
For at tablet.
Embodiment 8
20 parts of broken masson pine pollen, 25 parts of natural pollen pini, combination mixes according to the ratio, in addition pharmaceutically acceptable auxiliary material system
For at powder.
Embodiment 9
15 parts of broken masson pine pollen, 28 parts of natural pollen pini, combination mixes according to the ratio, in addition pharmaceutically acceptable auxiliary material system
For at granule.
Embodiment 10
12 parts of broken masson pine pollen, 20 parts of natural pollen pini, combination mixes according to the ratio, in addition pharmaceutically acceptable auxiliary material system
For at granule.
Pharmacodynamic experiment
1. the influence of pair mice with constipation
1.1 material
Kunming mouse (male and female dual-purpose, weight 18-20g), prepared Chinese ink, the present composition, broken masson pine pollen, natural preserved egg
Powder, compound diphenoxylate.
1.2 method
(1) be grouped: mouse is normally raised 5 days after buying back and is observed, without exception, selects 50 healthy mices and divides at random
At 5 groups, i.e., normal group, model group, broken masson pine pollen group, natural pollen pini group, present composition group (by embodiment 4 prepare
Broken wall+natural pollen pini group).
(2) dosage and administration route: oral stomach-filling, stomach-filling volume are 0.2ml/10g weight, and blank control group is given
The distilled water of amount.A weight of animals is claimed to adjust stomach-filling amount every other day.It was calculated according to pollen pini conventional amount used 3-6g/ days, in test
With adult (60kg), dosage 6g is calculated daily, and being converted to mouse bioequivalence dosage is daily 900mg/kg.
(3) modeling: taking R-1132 50mg (20), is ground with mortar and adds distilled water to be settled in after powder
100ml, for manufacturing Constipation Model.Experimental measuring is every mouse stomach-filling 0.2ml/10g weight, that is, 10mg/kg weight.Respectively
Stomach-filling laboratory sample, model group and Normal group distinguish stomach-filling normal salt to experimental group respectively in addition to handling according to model group
Water.
(4) testing index:
A Intestinal pushing functional experiment: after continuous gavage gives each group mouse difference tested material 14d, fasting 16h is during which free
Drinking-water.The compound diphenoxylate of 10mg/kg is given in stomach-filling respectively for Constipation Model group and three test groups.Negative control group is to consubstantiality
Product physiological saline.After 30min, the prepared Chinese ink suspension containing corresponding tested material is given in three test group stomach-fillings, negative control group and
Model group stomach-filling blank prepared Chinese ink, row cervical dislocation puts to death animal after 25min, take out rapidly mouse stomach and whole section of ileocecus position
Small intestine is not added traction and is directly laid on blank sheet of paper after gently removing, measurement Length of intestine is " total small intestinal length ", from pylorus to prepared Chinese ink
The distance in forward position is that prepared Chinese ink promotes length, and Intestinal propulsive rate (%) is calculated according to the following formula:
The experiment of B mouse defecation: mouse difference tested material is given in stomach-filling, and each group stool in mice character is observed during raising.It fills
14d after stomach, fasting are drunk water 16 hours.10mg/kg compound diphenoxylate is given in Constipation Model group and three test group stomach-fillings, right
Same volume physiological saline is given according to group.After 30min, the prepared Chinese ink suspension containing corresponding tested material is given in each test group stomach-filling, negative
Blank prepared Chinese ink is given in control group and model group stomach-filling.The equal single cage raising normal water feed of animal.The timing since prepared Chinese ink stomach-filling,
Record every animal clap the first grain melena time, defecation grain number and weight in 5h.
1.3 test results and analysis
Influence (n=10) of the 1 different disposal pollen pini of table to mouse small intestine propulsion rate
| Group | Total small intestinal length | Prepared Chinese ink promotes length | Ink progradation |
| Normal group | 58.68±8.24 | 35.12±5.74 | 60.16±8.50 |
| Model group | 59.84±4.21 | 25.46±5.76 | 42.38±8.86## |
| Broken masson pine pollen group | 57.62±6.55 | 28.44±6.66 | 53.31±7.06* |
| Natural pollen pini group | 54.82±3.55 | 33.08±4.23 | 59.47±4.31** |
| Present composition group | 56.04±4.30 | 42.68±6.32 | 76.86±6.88** |
The P compared with normal group##< 0.01, the P compared with model group*< 0.05, P**< 0.01
Influence (n=10) of 2 pollen pini of table to each defecation index of mouse
| Group | The just time non-for the first time | Defecation grain number in 5h | Defecation weight in 5h |
| Normal group | 115.6±12.8 | 24.6±8.8 | 0.79±0.12 |
| Model group | 187±21.7## | 11.2±2.3## | 0.28±0.11## |
| Broken pollen group | 162±16.2* | 20.1±2.8** | 0.45±0.14** |
| Natural pollen group | 141±11.8** | 24.7±1.9** | 0.58±0.12** |
| The present composition | 123±12.6** | 30.5±4.7** | 0.69±0.16** |
The P compared with normal group##< 0.01, the P compared with model group*< 0.05, P**< 0.01
Model group mouse small intestine ink progradation is significantly lower than Normal group it can be seen from upper table 1, shows mouse just
Secret model foundation success, each medicine-feeding test group can increase mice with constipation small intestine ink progradation, exist compared with model group aobvious
Work or extremely significant difference.It is the most significantly wherein the present composition, is secondly natural pollen pini group, is again broken wall pine
Pollen.As can be seen that comparing with normal group, the model group small mouse's head grain melena time is obviously prolonged table 2,5 hours melena quantity and
Weight significantly reduces, and illustrates modeling success.Three test groups, which are observed, can shorten the time that mice with constipation arranges first grain melena, increase
Add 5 hours row's melena quantity and weight, wherein the most obvious with present composition group.
The experimental result observed above may be related with the loss of the dietary fiber of broken masson pine pollen.Contain in pollen pini
The water-soluble dietary fibers such as pectin, soluble polysaccharide, oligosaccharide in enteron aisle at solution state, there is preferable retention ability,
And solution, by enteric bacteria glycolysis, enteron aisle can be reduced by generating short chain fatty acids, these short chain fatty acids such as butyric acid, propionic acid, acetic acid
Interior environmental pH, stimulation intestinal mucosa are wriggled.The carbon dioxide that is generated after furthermore water-soluble dietary fiber is fermented by intestinal flora,
The gases such as hydrogen, methane can also stimulate enterocinesia, to accelerate excrement discharge.Lignin, cellulose in pollen pini, hemicellulose
Equal insoluble diedairy fibers have stronger water absorbing force and swellability, and are not easy microorganism in digestive ferment or enteron aisle by alimentary canal
Glycolysis can form more food residue and increase stool weight and volume, keep excrement soft, be easy to be discharged.Excrement weight
The increase of amount and volume mechanically can stimulate intestinal wall to cause awareness of defecation, accelerate intestines peristalsis, so as to shorten swill in intestines
By the time in road, the generation of constipation is prevented.
2. influencing to test on intestinal flora
2.1 material
Kunming mouse, the present composition, broken masson pine pollen, natural pollen pini, Escherichia coli culture medium (EMB culture
Base), enterococcus culture medium (EC culture medium), Bifidobacterium (BBL culture medium), Bacillus acidi lactici culture medium (MRS culture medium) etc..
2.2 method
After mouse adapts to raising 4d, start formal test.Healthy mice 40 are selected, 4 groups (every group 10) are randomly divided into,
It is respectively as follows: control group, broken masson pine pollen group, natural pollen pini group, present composition group.The stomach-filling of each test group difference accordingly by
Try object, control group stomach-filling same amount of normal saline.
Before stomach-filling and stomach-filling the 14th day, sterile collection fresh excrement sample 0.1-0.4g is put into dry sterilization after weighing
In bottle, with 1:100 ratio, add sterile water, uniform suspension be made, then successively 10 times be incremented by and be diluted to a series of different dilutions
Containing bacterium solution to 10-9, sample dilution processing is completed in 4h.According to the result of preliminary experiment, 3 suitable bacterium solution dilutions are selected,
Lml is taken respectively, and on each selective medium, each dilution sets two repetitions.After bacterium colony is grown, to 4 kinds of intestinal floras
It is analyzed, (loglO is as a result indicated with the logarithm of the bacterium colony in every gram of excrementnCFU/g).4 kinds of bacterium cultivate 48h at 37 DEG C
After count.Wherein, Bifidobacterium and lactobacillus Anaerobic culturel, the identification technology method of various bacterium: lactobacillus is microscopy G+, shape
The bacterium colony of state rule；Bifidobacterium is microscopy G+, the irregular bacterium colony of form；Enterococcus is microscopy G+, the irregular bacterium of form
It falls；Enterobacteria is atropurpureus with or without green metal gloss, microscopy G-Bacterium colony.
2.3 test results and analysis
Influence of 3 pollen pini of table to intestinal flora
Compared with Normal group, P*< 0.05, P**< 0.01；Compare P before and after stomach-filling#< 0.05, P##< 0.01
The above result shows that after mouse is continuously fed each tested material 14d, Bifidobacterium, lactobacillus in enteron aisle, large intestine
Advantageous variation has occurred in bacillus and enterococcus quantity to some extent.I.e. Bifidobacterium, lactobacillus have apparent increase trend,
And Escherichia coli, enterococcus have apparent reduction trend.Do not gavage before and after each flora stomach-filling of blank control group of tested material all without
The significance of difference.This phenomenon is the most it is apparent that the present composition.
Although aforementioned present invention composition is the experimental data of composition prepared by embodiment 4, but invention of the invention
People has carried out above-mentioned reality as tested material to broken masson pine pollen in other embodiments 5- embodiment 10 and natural pine pollen composition
It tests, the results show that almost the same with the trend of above-mentioned data, this specification selection is experiment effect it is still further preferred that embodiment 4
Portfolio ratio.
Survive a large amount of bacterium in human body intestinal canal, these bacteriums form a complicated microecosystem, enterobacteriaceae
For group by forming to the beneficial and harmful two major classes of human health, Bifidobacterium and lactobacillus are that beneficial microorganism represents, and can be pressed down
The undue growth of pathogen processed is bred, and immunity of organisms is enhanced.And enterococcus and Escherichia coli are considered as normal intestinal flora
Structure is the representative of harmful bacteria to the important warning factor for being unfavorable for the transformation of body health direction.The extraordinary increasing of harmful bacteria quantity
Add and may cause certain intestines problems, therefore the normal environment of the Tiny ecosystem between maintenance intestinal flora and human health are closely related
Also most important.In experiment, the situation of change of the above-mentioned four major class intestinal flora of the mouse of the corresponding tested material of stomach-filling is to being conducive to
The direction of its health is developed.Especially the present composition has obvious inhibiting effect to pernicious bacteria, has growth-promoting to profitable strain
Effect.This may can generate a large amount of organic acid with the participation of the numerous microorganisms during the fermentation of dietary fiber in pollen pini,
PH value in enteron aisle is reduced, to inhibit the growth and breeding of pathogenic bacteria related.
Claims (5)
1. improving the pine pollen composition of gastrointestinal bacterial flora or relax bowel and defecation, it is characterised in that: it is comprising following weight proportion
The preparation of suitable clinical use that is prepared plus pharmaceutically acceptable auxiliary material or complementary ingredient of active constituent:
12 parts of broken masson pine pollen, 16 parts of natural pollen pini.
2. the pine pollen composition according to claim 1 for improving gastrointestinal bacterial flora or relax bowel and defecation, it is characterised in that: institute
State natural pollen pini be masson pine, Chinese pine or other belong to one of pollen or a variety of.
3. the pine pollen composition according to claim 1 or 2 for improving gastrointestinal bacterial flora or relax bowel and defecation, feature exist
In: the broken masson pine pollen is the pollen pini of 95% or more sporoderm-broken rate.
4. the medicine that the described in any item pine pollen compositions of claims 1 to 3 improve gastrointestinal bacterial flora or relax bowel and defecation in preparation
Purposes in object or health care product.
5. purposes according to claim 4, the relax bowel and defecation refers to enhancing WeiDongLi Capsule, promotes gastric emptying or promotes enteron aisle
It wriggles, the improvement gastrointestinal bacterial flora, which refers to, improves micro ecology of gastrointestinal tract environment.
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| CN105918731A (en) * | 2016-05-16 | 2016-09-07 | 合肥睿联生物科技有限公司 | Squid bone functional beverage with gastrointestinal tract adjusting function and preparing method thereof |
| CN109497483A (en) * | 2018-01-26 | 2019-03-22 | 中国科学院烟台海岸带研究所 | One kind is containing phycocyanin and pine pollen composition and its preparation and application |
| RU2713175C1 (en) * | 2019-02-04 | 2020-02-04 | Виктор Анатольевич Маткевич | Acid-mineral composition and enteral solution by matkevich for lavage of gastrointestinal tract and/or correction of homeostasis disorders |
| CN110089708A (en) * | 2019-05-16 | 2019-08-06 | 郝景龙 | Bi-component broken masson pine pollen |
| CN110122887A (en) * | 2019-06-03 | 2019-08-16 | 中国农业科学院农产品加工研究所 | Improve the dietary fiber chewable tablets and preparation method thereof of gastrointestinal function |
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| 松花粉作为保健食品的优势和特点;石丽花等;《农业工程技术（农产品加工业）》;20081231(第1期);第20-24页，尤其是第23页左栏第4-5段，20页左栏第4段 |
| 松花粉破壁前后显微形态和营养成分的研究;赵霖等;《营养学报》;20011231;第23卷(第2期);第153-156页，尤其是第155页右栏第3段 |
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