KR101199556B1 - Extract Of Eriobotrya japonica Seed-Containing Cosmetic Composition Having Anti-aging Effect - Google Patents

Abstract

The present invention relates to an anti-aging cosmetic composition containing a loquat (Eriobotrya japonica seed) extract. The anti-aging cosmetic composition is excellent in antioxidant effect, biosynthetic effect of collagen fibers, activity inhibitory effect of hyaluronicase. In addition, the cosmetic prepared by using the anti-aging cosmetic composition is not only excellent skin elasticity enhancing effect, but also contains a natural extract loquat extract is also excellent for safety to the skin.

Loquat extract, cosmetics, anti-aging, antioxidant, collagen fiber, hyaluronase

Description

Anti-aging cosmetic composition containing loquat extract {Extract Of Eriobotrya japonica Seed? Containing Cosmetic Composition Having Anti-aging Effect}

The present invention relates to an anti-aging cosmetic composition, and more particularly, to an anti-aging cosmetic composition containing Eriobotrya japonica Seed extract.

The skin aging mechanism, which is the basis for the development of anti-aging cosmetics, is continuously exposed to oxidative stress from the harmful environment such as air pollution, UV exposure, stress or disease, and the radicals in the body are increased. It can be explained that the breakdown of collagen, collagen, elastin, hyaluronic acid and the like causes subsidence of the skin (wrinkle). In addition, the skin aging mechanism oxidizes the lipid portion of the cell membrane, causing cell destruction, and may cause diseases such as dermatitis, acne or skin cancer. In addition, radicals in the body may be involved in the melanin formation process, causing blemishes, freckles and wrinkles.

Regarding the skin aging mechanism, attention has been paid to systemic and endogenous physiological aging according to age, and to local exogenous photoaging due to prolonged exposure to ultraviolet light. In particular, the face, head, and the like, which are important parts of beauty, have been found to be greatly affected by photoaging, and studies on photoaging and wrinkle formation of human skin and animal models have been actively conducted. The internal structure of the skin changes with photoaging, for example, epidermis, thickening of the dermis or accumulation of elastin, or three-dimensional structure change of elastic fibers has been reported. In addition, in relation to collagen, a major constituent skeleton of skin structure, microfiberization of fibrous collagen fibers and weakening of fibrous form have been revealed. uvB-induced damage. J. Invest.dermatol., 100, 194-199 (1993)) However, the collagen fiber bundle formed by the collagen fibers is sufficiently examined until now for the structural change due to photoaging, the change in skin properties, etc. It is only known that it affects the toughness of the skin and the like by the study of the skin wound treatment process or the like.

  Recently, studies on the direct and indirect effects of sunlight due to the destruction of the ozone layer, which has been found to be the mainstream of skin aging research, are being conducted. In particular, research on the effect of collagen fibers using various herbal ingredients in order to find out the effect of the sunlight on the formation of wrinkles is actively underway.

  Existing methods for preventing skin aging or skin wrinkle improvement have been to prepare and use cosmetics or pharmaceutical products containing ascorbic acid, α-tocopherol, retinol and derivatives thereof, SOD and the like. However, the price of such cosmetics and medicines is not only expensive, but the chemical stability is not good, so it is difficult to expect a substantial effect in the cosmetics industry as well as in the medicine and food sectors.

An object of the present invention for solving the above problems is to provide an anti-aging cosmetic composition containing a natural material that is not expensive and safe for the human body.

In addition, an object of the present invention is to provide an anti-aging cosmetic composition having an effect of antioxidant activity, biosynthetic effect of collagen fibers, as well as the effect of lowering the activity of hyaluronase.

Anti-aging cosmetic composition of the present invention for achieving the above object contains loquat (Eriobotrya japonica Seed) extract.

The loquat extract preferably extracts loquat using an extraction solvent obtained by mixing ethanol, butylene glycol and distilled water in a weight ratio of 2: 1: 1.

The loquat extract is preferably contained 0.001 to 20% by weight relative to the total weight of the anti-aging cosmetic composition.

Anti-aging cosmetic composition containing a loquat extract of the present invention is excellent in antioxidant activity, biosynthetic effect of collagen fibers as well as inhibiting the activity of hyalurase.

In addition, the anti-aging cosmetics containing loquat extract of the present invention not only has an effect of enhancing skin elasticity, but also contains a loquat extract, which is a natural extract, and also has excellent safety for the skin.

The present invention relates to an anti-aging cosmetic composition containing a loquat (Eriobotrya japonica Seed) extract. The loquat (Eriobotrya japonica Seed) refers to the seed of the loquat, the loquat (Eriobotrya japonica) is an evergreen tree belonging to the Rosaceae (Rosaceae), its fruit, root, stem bark and leaves are mainly used in herbal medicine for medicinal use do.

Preferably, the loquat extract is preferably extracted with loquat by an extraction solvent in which ethanol, butylene glycol and distilled water are mixed in a weight ratio of 2: 1: 1. However, not only the extraction solvent but also the wave can be extracted using a solvent that is commonly used during extraction. In addition, not only the above-mentioned ethanol, but also lower alcohols having 1 to 4 carbon atoms can also be used anhydrous alcohol (hydrogen alcohol) or hydrous alcohol (含水 alcohol).

The loquat extract is preferably contained 0.001 to 20% by weight relative to the total weight of the anti-aging cosmetic composition.

Hereinafter, with reference to the preferred production examples, comparative examples of the present invention will be described in detail. The following Preparation Examples and Comparative Examples are only presented to understand the contents of the present invention, and those skilled in the art will be capable of many modifications within the technical spirit of the present invention. Therefore, the scope of the present invention should not be construed as being limited to these production examples, comparative examples.

Preparation Example 1 Preparation of Loquat Extract Using Extraction Solvent Containing Ethanol, Butylene Glycol and Distilled Water

Loquat extract contained in the anti-aging cosmetic composition of the present invention is prepared by the following process.

First, the loquat, which is a seed of the loquat tree native to Korea, was purchased and washed with distilled water, and then completely dried in a cool place without sunlight until there was no change in weight. Thereafter, the completely dried loquat was physically ground using a homogenizer (AM-7, Hyronchem) to obtain loquat pulverized product.

Next, ethanol: butylene glycol: distilled water was mixed in a weight ratio of 2: 1: 1 to prepare 1500 g of an extraction solvent, and then 100 g of the loquat pulverized product was added. At this time, the mixing ratio of the loquat pulverized product and the extraction solvent was 1:20 by weight ratio. However, in the present production example, the loquat pulverized product and the extraction solvent were mixed in a weight ratio of 1:20, but can be freely mixed at a ratio of from 1:10 to 1:30. Extraction efficiency is good when extracting in the range of said mixing ratio.

Next, the extraction solvent mixed with the loquat pulverized product was put into an extractor equipped with a reflux condenser and extracted at 60 ° C. for 7 hours. The material obtained by the extraction was subjected to primary extraction solvent using a 200 mesh filter cloth (Cheongggye Sangyong) and subjected to secondary filtration using whatman No. 2 extraction solvent at room temperature to remove insoluble matters. Thereafter, the mixture was concentrated under reduced pressure at 50 ° C. in a distillation apparatus equipped with a cooling condenser, and then lyophilized to obtain a loquat extract of the present invention. The loquat extract was 16.2 g in dry weight.

Preparation Example 2 Preparation of Loquat Extract Using Extraction Solvent Containing Ethanol and Distilled Water

In order to compare with the loquat extract of the present invention of Preparation Example 1, loquat extract was prepared using a different extraction solvent from Preparation Example 1.

 To prepare a loquat extract in the same manner as in Preparation Example 1, the extraction solvent was used was mixed in a weight ratio of ethanol: distilled water = 3: 1: As a result, 18.4 g of loquat extract was obtained by dry weight.

Preparation Example 3 Preparation of Loquat Extract Using Extraction Solvent Containing Butylene Glycol and Distilled Water

In order to compare with the loquat extract of the present invention of Preparation Example 1, loquat extract was prepared using a different extraction solvent from Preparation Example 1.

 To prepare a loquat extract in the same manner as in Preparation Example 1, the extraction solvent was used was mixed in a weight ratio of butylene glycol: distilled water = 3: 1: As a result, 14.6 g of loquat extract was obtained by dry weight.

Preparation Example 4 Preparation of Nutrition Cream

Nutritional creams were prepared using the loquat extracts prepared in Preparation Examples 1 to 3 above, but the loquat extract was contained in an amount of 8.00 wt% based on the total weight of the nutrition cream.

First, according to the composition ratio of the following Table 1, purified water, loquat extract, carbomer (Carbomer), disodium EDTA, butylene glycol (Butylene Glycol), methyl paraben (Methyl Paraben) by weighing 80 ± Mixing at 2 ° C. completes the water phase. Further, according to the composition ratio of Table 1, Propyl Paraben, Macademia Nut Oil, Mineral Oil, Dimethicon, Caprylic Capric Triglyceride (Caprylic) Capric Triglyceride) was weighed and mixed at 80 ± 2 ° C. to complete the oil phase.

Next, the oil phase was added to the water phase and the two phases were mixed with each other to form a mixture in emulsion form. Triethanolamine, a neutralizing agent, was added to the mixture and cooled to 45 ° C., followed by the addition of perfume (Argeville, M.A. CO 25106B) and cooling to 35 ° C. to prepare a nourishing cream.

At this time, three kinds of nutrition cream 1, nutrition cream 2, nutrition cream 3 using the loquat extract of Preparation Examples 1 to 3. As a control, without using the loquat extract, the weight ratio of purified water to 84.95%, according to the composition ratio of Table 1 below to prepare a control nutrition cream.

[Table 1]

division Raw material name weight(%) Nutrition Cream 1 Nutrition Cream 2 Nutrition Cream 3 Control group
Nutrition Cream Awards One  Purified water 76.95 76.95 76.95 84.95 2  Loquat extract of Preparation Example 1 8.00 3  Loquat extract of Preparation Example 2 8.00 4  Loquat extract of Preparation Example 3 8.00 5  Carbomer 0.15 0.15 0.15 0.15 6  Disodium ID 0.05 0.05 0.05 0.05 7  Butylene glycol 4.00 4.00 4.00 4.00 8  Methyl paraben 0.20 0.20 0.20 0.20 Paid 9  Propylparaben 0.10 0.10 0.10 0.10 10  Macadamia Nut Oil 4.00 4.00 4.00 4.00 11  Mineral oil 3.00 3.00 3.00 3.00 12  Dimethicone 1.00 1.00 1.00 1.00 13  Caprylic Capric Triglycerides 2.00 2.00 2.00 2.00 corrector 14  Triethanolamine 0.25 0.25 0.25 0.25 additive 15  Spices 0.30 0.30 0.30 0.30

Production Example 5 Preparation of Flexible Lotion

Using the loquat extracts prepared in Preparation Examples 1 to 3 to prepare a flexible lotion, but was added to 8.00% by weight of the loquat extract relative to the total weight of the flexible lotion.

First, according to the composition ratio of Table 2, purified water, loquat extract, glycerin (Glycerin), sodium PCA solution was measured and mixed at 80 ± 2 ℃ to complete the water phase (water phase). In addition, according to the composition ratio of Table 2 below, denatured alcohol (95% ethanol), methyl paraben (Methyl Paraben), propyl paraben (Propyl Paraben) was measured and mixed at 80 ± 2 ℃ to complete the alcohol phase (alcohol phase) It was.

Next, after mixing the water phase (alcohol phase) and the alcohol phase (alcohol phase) to make a mixture, and mixed with a flavor (Argeville, MA CO 25106B), the membrane filter (Samdeok Filtration Co., Paper) Impurities were filtered through a filter) to prepare a flexible lotion.

At this time, three kinds of flexible lotion 1, flexible lotion 2, and flexible lotion 3 were prepared using the loquat extracts of Preparation Examples 1 to 3. As a control, without using the loquat extract, the weight ratio of purified water to 85.81%, to prepare a control flexible lotion according to the composition ratio of Table 2 below.

[Table 2]

division Raw material name
weight(%) Flexible lotion1 Flexible Cosmetic 2 Flexible Cosmetic 3 Control group
Flexible Cosmetics Awards




One Purified water 77.81 77.81 77.81 85.81 2 Loquat extract of Preparation Example 1 8.00 3 Loquat extract of Preparation Example 2 8.00 4 Loquat extract of Preparation Example 3 8.00 5 glycerin 1.00 1.00 1.00 1.00 6 Sodium PCA Solution 3.00 3.00 3.00 3.00 Alcoholic

7 denatured alcohol 10.00 10.00 10.00 10.00 8 Methyl paraben 0.10 0.10 0.10 0.10 9 Propylparaben 0.02 0.02 0.02 0.02 additive 10 Spices 0.07 0.07 0.07 0.07

Production Example 6 Preparation of Nutritional Lotion

Nutritional lotion was prepared using the loquat extract prepared in Preparation Examples 1 to 3 above, but the loquat extract was contained in an amount of 8.00 wt% based on the total weight of the nutrition lotion.

First, according to the composition ratio of Table 3, purified water, loquat extract, carbomer, disodium idieti, butylene glycol, methyl parabens were measured and mixed at 80 ± 2 ℃ to complete the water phase (water phase). In addition, according to the composition ratio of Table 3 below, propylparaben, macadamia nut oil, mineral oil, dimethicone, sorbitan sesquioleate, polysorbate 60 (Polysorbate 60) was measured and 80 The oil phase was completed by mixing at ± 2 ° C.

Next, the oil phase was added to the water phase and the two phases were mixed with each other to form a mixture in emulsion form. Triethanolamine, a neutralizing agent, was added to the mixture and cooled to 45 ° C., followed by the addition of perfume (Argeville, M.A. CO 25106B) and cooled to 35 ° C. to prepare a nourishing lotion.

At this time, three kinds of nourishing lotion 1, nutrient lotion 2, and nutrient lotion 3 were prepared using the loquat extracts of Preparation Examples 1 to 3. As a control, without using the loquat extract, the weight ratio of purified water to 86.13%, according to the composition ratio of Table 3 below to prepare a control nutrition lotion.

[Table 3]

division Raw material name weight(%) Nourishing Lotion 1 Nutrition lotion 2 Nutrition lotion 3 Control group
Nutrition lotion Awards One  Purified water 78.13 78.13 78.13 86.13 2  Loquat extract of Preparation Example 1 8.00 3  Loquat extract of Preparation Example 2 8.00 4  Loquat extract of Preparation Example 3 8.00 5  Carbomer 0.15 0.15 0.15 0.15 6  Disodium ID 0.02 0.02 0.02 0.02 7  Butylene glycol 1.00 1.00 1.00 1.00 8  Methyl paraben 0.20 0.20 0.20 0.20 Paid 9  Propylparaben 0.10 0.10 0.10 0.10 10  Macadamia Nut Oil 2.00 2.00 2.00 2.00 11  Mineral oil 6.00 6.00 6.00 6.00 12  Dimethicone 2.00 2.00 2.00 2.00 13  Sorbitan Sesquioleate 0.40 0.40 0.40 0.40 14  Polysorbate 60 1.60 1.60 1.60 1.60 corrector 15  Triethanolamine 0.15 0.15 0.15 0.15 additive 16  Spices 0.25 0.25 0.25 0.25

Preparation Example 7 Preparation of Essence

Essence was prepared using the loquat extracts prepared in Preparation Examples 1 to 3 above, but the loquat extract was contained in an amount of 8.00 wt% based on the total weight of the essence.

First, according to the composition ratio of Table 4, purified water, loquat extract, methyl paraben, hyaluronic acid extract, glycerin was measured and mixed at 80 ± 2 ℃ to complete the water phase (water phase). In addition, according to the composition ratio of Table 4 below, propylparaben, lecithin, macadamia nut oil was measured and mixed at 80 ± 2 ℃ to complete the oil phase (oil phase).

Next, the oil phase was added to the water phase and the two phases were mixed with each other to form a mixture in emulsion form. Essence was prepared by adding flavor (Argeville, M.A. CO 25106B) to the mixture and cooling to 35 ° C.

At this time, three kinds of essence 1, essence 2, essence 3 were prepared using the loquat extracts of Preparation Examples 1 to 3. As a control, a weight ratio of purified water was 78.58% without using a loquat extract, and a control essence was prepared according to the composition ratio of Table 4 below.

[Table 4]

division Raw material name weight(%) Essence 1 Essence 2 Essence 3 Control group
essence Awards One  Purified water 70.58 70.58 70.58 78.58 2  Loquat extract of Preparation Example 1 8.00 3  Loquat extract of Preparation Example 2 8.00 4  Loquat extract of Preparation Example 3 8.00 5  Methyl paraben 0.15 0.15 0.15 0.15 6  Hyaluronic Acid Extract 2.50 2.50 2.50 2.50 7 glycerin 8.00 8.00 8.00 8.00 Paid 8  Propylparaben 0.10 0.10 0.10 0.10 9  lecithin 0.60 0.60 0.60 0.60 10  Macadamia Nut Oil 10.0 10.0 10.0 10.0 additive 11  Spices 0.07 0.07 0.07 0.07

Comparative Example 1 Antioxidant Effect of Loquat Extract

In order to confirm that the loquat extract prepared by Preparation Examples 1 to 3 has an antioxidant effect, a thiobutylate acetic acid (TBA) test was performed using a lipid peroxidation system by the Fenton reaction.

First, 25 mM Trizma hydrochloric acid / 0.75 mM potassium chloride buffer solution was prepared by adding 0.2% sodium dodecyl sulfoxide (SDS), 10 mol of ferric chloride, and 2 mol of hydrogen peroxide to 10 mL of ethyl linoleate.

Next, 0.01 g of the loquat extract of Preparation Example 1 was dissolved in 0.1 mL of dimethyl sulfoxide (DMSO) solution. After mixing the solution of the loquat extract and 5 mL of 25 mM Trisma hydrochloric acid / 0.75 mM potassium chloride buffer, incubated at 55 ℃ for 16 hours, and added 50 mL of 4% butylhydroxy toluene The reaction was stopped and then placed in a 0.3 mL test tube. 3.0 mL of 0.05M hydrochloric acid and 4.0 mL of butanol were added to the 0.3 mL test tube, and centrifuged to measure the absorbance of the sample at 535 nm.

In addition, the samples containing the loquat extract of Preparation Example 2 and loquat extract of Preparation Example 3, through the same steps as described above, the absorbance at 535 nm was measured.

And the absorbance was measured at 535 nm using the sample which does not contain the loquat extract of manufacture example 1 thru | or manufacture example 3 as a control. The measurement result of absorbance is shown in Table 5 below.

[Table 5]

sample Absorbance Contains loquat extract of Preparation Example 1 0.145 Contains loquat extract of Preparation Example 2 0.142 Contains loquat extract of Preparation Example 3 0.123 Control group 0.080

Using the absorbance of Table 5, the antioxidant effect was calculated. Antioxidant effect can be calculated by the following formula, the results are shown in Table 6 below.

TABLE 6

sample Antioxidative effect Contains loquat extract of Preparation Example 1 81.25 Contains loquat extract of Preparation Example 2 77.50 Contains loquat extract of Preparation Example 3 53.75 Vitamin E 45.20

As can be seen from the results of Table 6, the loquat extract of Preparation Example 1 (original invention) obtained by using an extraction solvent prepared by mixing ethanol: butylene glycol: distilled water in a weight ratio of 2: 1: 1 has an antioxidant effect Was 81.25. On the other hand, the anti-oxidative effect of the loquat extract of Preparation Example 2 was 77.50, and the antioxidant effect of the loquat extract of Preparation Example 3 was 53.75. The antioxidant effect of vitamin E, which is widely known to have an antioxidant effect, was only 45.20. From the above results, it can be seen that the antioxidant effect of the loquat extract of the present invention is very high.

<Comparative Example 2>: Collagen fiber biosynthesis effect of loquat extract

A test was conducted to confirm that the loquat extract prepared in Preparation Examples 1 to 3 had an effect of biosynthesizing collagen fibers.

First, normal human fibroblasts (ATCC-28) were placed in a culture flask and cultured until 70-80% growth. The cultured fibroblasts were treated with the loquat extracts of Preparation Examples 1 to 3, respectively, at three ratios of 0.01 wt%, 0.10 wt% and 1.00 wt% relative to the total weight of the fibroblasts. Thereafter, the collagen fiber biosynthesis rate (%) of the fibroblasts treated with the loquat extract was measured using a commercially available collagen fiber biosynthesis kit (BCA protein assay kit, pierce 23225).

In addition, fibroblasts not treated with the loquat extracts of Preparation Examples 1 to 3 were used as a control group, but the control group 1 was used when no ultraviolet rays were irradiated, and the control group 2 was irradiated with UV rays. After coarse, the percentage of collagen fiber biosynthesis of fibroblasts was measured.

The collagen fiber biosynthesis rate (%) measured above is shown in Table 7 below.

[Table 7]

sample Collagen Fiber Biosynthesis (%) Loquat Extract
(0.01 wt%) Loquat Extract
(0.10 wt%) Loquat Extract
(1.00 wt%) Loquat extract of Preparation Example 1
Treated fibroblasts 158 170 189 Loquat extract of Preparation Example 2
Treated fibroblasts 115 130 141 Loquat extract of Preparation Example 3
Treated fibroblasts 99 110 119 Control group 1 96 96 96 Control 2 68 68 68

As can be seen from the results of Table 7, the ethanol: butylene glycol: distilled water was treated with the loquat extract of Preparation Example 1 (invention) obtained by using an extraction solvent prepared by mixing in a weight ratio of 2: 1: 1. The percentage of collagen fiber biosynthesis of fibroblasts was much higher than that of other fibroblasts. The collagen fiber biosynthesis rate (%) was more than twice that of the control group 1 or the control group 2 not treated with loquat extract.

In addition, when the fibroblasts were treated with the loquat extract of Preparation Example 2 or 3, the collagen fiber biosynthesis rate (%) when treated with the loquat extract of 1.00% by weight relative to the total weight of the fibroblasts was 141% and 119%, respectively. It was. On the other hand, when the fibroblasts were treated with the loquat extract of the Preparation Example 1 (invention of the present invention), the collagen fiber biosynthesis rate (%) reached 158% even when only 0.01% by weight of the loquat extract relative to the total weight of the fibroblasts.

As can be seen from the above, the fact that the high rate of collagen fiber biosynthesis of fibroblasts treated with the loquat extract of the present invention shows that the loquat extract of the present invention has an excellent effect in preventing wrinkles caused by aging, especially photoaging. will be.

<Comparative Example 3>: Inhibitory effect of hyaluronicase of loquat extract

The loquat extract prepared by Preparation Examples 1 to 3 was tested to confirm that it has an effect of inhibiting the activity of hyaluronicase (sigma, type 4). The hyaluronicase is an enzyme that decomposes hyaluronic acid involved in the water retention of the cell. When the activity of the hyaluronicase is inhibited, the stability of the hyaluronic acid is increased, and thus the water retention capacity of the cell may be increased.

First, 10 mg of the loquat extract of Preparation Example 1 was dissolved in 100 mL of 0.1 M acetic acid buffer (pH 4.0) and placed in a test tube. Thereafter, 50 mL of acetic acid in which 0.1 mg of hyaluronase was dissolved was added to the test tube, and left at 37 ° C. for 20 minutes.

Next, 250 mL of acetic acid buffer in which 200 mg of sodium hyaluronate was dissolved was added to the test tube, and left at 37 ° C. for 40 minutes. Thereafter, 100 mL of 0.4 M sodium hydroxide was added to the test tube and cooled.

Next, 100 mL of boric acid buffer solution (pH 9.0) was added to the test tube, and the absorbance was measured at 585 nm of the sample incubated at 37 ° C. for 20 minutes. In addition, for the samples containing the loquat extract of Preparation Example 2 and loquat extract of Preparation Example 3, the absorbance was measured at 585 nm through the same steps as described above.

And the absorbance was measured at 585 nm using the sample which did not contain the loquat extract of manufacture example 1 thru | or manufacture example 3 as a control. The measurement result of absorbance is shown in following Table 8.

[Table 8]

sample Absorbance Contains loquat extract of Preparation Example 1 0.195 Contains loquat extract of Preparation Example 2 0.181 Contains loquat extract of Preparation Example 3 0.168 Control group 0.102

Using the absorbance of Table 8, the activity inhibitory effect of hyaluronicase was calculated. The inhibitory effect of hyaluronicase can be calculated by the following formula, and the results are shown in Table 9 below.

TABLE 9

sample Inhibitory Effect of Hyaluronase (%) Contains loquat extract of Preparation Example 1 91.17 Contains loquat extract of Preparation Example 2 77.45 Contains loquat extract of Preparation Example 3 64.71

As can be seen from the results of Table 9, hyaluronic agent of the loquat extract (Preparation of the present invention) of Preparation Example 1 obtained by using an extraction solvent prepared by mixing ethanol: butylene glycol: distilled water in a weight ratio of 2: 1: 1. The inhibitory effect of was 91.17%. On the other hand, the inhibitory effects of hyaluronicase of the loquat extracts of Preparation Example 2 and Preparation Example 3 were 77.45% and 64.71%, respectively. From the above results, it can be seen that the loquat extract of the present invention has a very high effect of inhibiting the activity of hyaluronase.

<Comparative Example 4>: Nutritional cream enhances skin elasticity

The nutrition cream 1 prepared in Preparation Example 4 to 3 and the control cream was tested to determine whether there is an effect to promote skin elasticity.

For the test, 32 healthy women (average age 34 years) of 28 years old or older and 40 years old were selected and divided into 4 groups, and each group was provided with nutrition cream 1, nutrition cream 2, nutrition cream 3, and control nutrition cream. Was used twice daily for 12 weeks.

Skin elasticity before using the nourishing cream (0 weeks) and skin elasticity after using the nourishing cream for 12 weeks is a skin elasticity measuring instrument (Cutometer SEM575, Courage + at a temperature of 20-25 ° C. and a relative humidity of 60%). Khazaka) was used for the measurement. The elastic modulus (Net elasticity, R5) can be calculated using the measured values, and the closer the elastic modulus to 1, the better the skin elasticity enhancing effect. The actual elastic modulus is calculated by the following equation, the results are shown in Table 10.

Actual modulus of elasticity (R5) = R5 (12 weeks)-R5 (0 weeks)

[Table 10]

Experimental group Actual modulus of elasticity (R5) When using Nourishing Cream 1 0.19 When using Nourishing Cream 2 0.09 When using Nourishing Cream 3 0.05 In case of using control nutrition cream 0.015

As can be seen from the results in Table 10, the actual modulus of elasticity in the case of using the nourishing cream 1 containing the loquat extract (original invention) of Preparation Example 1 was 0.19. The actual modulus of elasticity when using the nourishing cream 1 was 210% higher than that of the nourishing cream 2 containing the loquat extract of Preparation Example 2, and 380 compared to the case of using the nourishing cream 3 containing the loquat extract of Preparation Example 3 The percentage was higher and was 1267% higher than the control nutrition cream containing no loquat extract. In conclusion, it was found that the nutrition cream containing loquat extract of the present invention effectively enhances skin elasticity.

Comparative Example 5 Safety of Nutrition Cream

The test was performed to determine whether the nutrition cream 1 to nutrition cream 3 containing the loquat extract prepared in Preparation Example 4 is safe for human skin.

For the test, adult men and women (15 males, 15 females) were selected and the nourishing cream 1, the nourishing cream 2, and the nourishing cream 3 were applied to the inner soft skin of the upper arm. After 24 hours and 48 hours after the application of the nourishing cream, the skin condition (skin irritation, erythema, itching, allergy, etc.) was observed, and the result (number of subjects showing positive reaction / number of total subjects). ) Is shown in Table 11 below.

TABLE 11

Experimental group After 24 hours After 48 hours When using Nourishing Cream 1 0/30 0/30 When using Nourishing Cream 2 0/30 1/30 When using Nourishing Cream 3 0/30 0/30

As can be seen from the results in Table 11 above, no one showed positive reaction to skin irritation, erythema, itching, allergies, etc. after 24 hours of application of nutrition cream 1, nutrition cream 2, and nutrition cream 3 After 48 hours, there was only one subject who tested positive for skin irritation, erythema, itching, and allergies only when nutrition cream 2 was used. In general, it can be seen that loquat extract is safe for the skin.

In particular, none of the subjects using the Nourishing Cream 1 containing the loquat extract (invention of the present invention) of Preparation Example 1 had a positive reaction against skin irritation, erythema, itching, allergy and the like. As a result, it was confirmed that the nutrition cream containing the loquat extract of the present invention has excellent safety for human skin.

<Comparative Example 6>: skin elasticity enhancing effect of the flexible lotion

Test to determine whether the flexible lotion 1 to 3 and the control flexible lotion prepared in Preparation Example 5 has the effect of improving skin elasticity. Actual elastic modulus (R5) obtained by the same method as in Comparative Example 4 was shown in Table 12 below.

[Table 12]

Experimental group Actual modulus of elasticity (R5) When using flexible lotion 1 0.100 If you use flexible lotion 2 0.030 When using flexible lotion 3 0.010 When using a control flexible lotion 0.007

As can be seen from the results in Table 12, the actual modulus of elasticity was 0.100 when the flexible lotion 1 containing the loquat extract of the Preparation Example 1 (original invention) was used. The actual modulus of elasticity when the flexible lotion 1 was used was 330% higher than when the flexible lotion 2 containing the loquat extract of Preparation Example 2 was used, and 1000 compared to the case where the flexible lotion 3 containing the loquat extract of Preparation Example 3 was used. % High, 1428% higher than the control lotion containing no loquat extract. In conclusion, it was found that the flexible lotion containing the loquat extract of the present invention effectively enhances skin elasticity.

Comparative Example 7 Safety of the Flexible Lotion

A test was performed to determine whether the flexible lotion 1 to 3 prepared lotion 3 prepared in Preparation Example 5 were safe for human skin. The results obtained by testing in the same manner as in Comparative Example 5 are shown in Table 13 below.

[Table 13]

Experimental group After 24 hours After 48 hours When using flexible lotion 1 0/30 0/30 If you use flexible lotion 2 0/30 1/30 When using flexible lotion 3 1/30 1/30

As can be seen from the results in Table 13, after 48 hours after the application of the flexible lotion 2 and 3, there were one subject who showed a positive response to skin irritation, erythema, itching, allergy and the like. However, none of the subjects who used the flexible lotion 1 containing the loquat extract of the Preparation Example 1 (the present invention) showed a positive response to skin irritation, erythema, itching, and allergy even after 48 hours. From the above results, it was confirmed that the flexible lotion containing the loquat extract of the present invention is very safe for human skin.

<Comparative Example 8>: Skin elasticity enhancing effect of nourishing lotion

Nutritional lotion 1 to 3 and the control nutritional lotion prepared in Preparation Example 6 was tested to determine whether the effect to enhance the skin elasticity. The actual modulus of elasticity (R5) obtained by the test in the same manner as in Comparative Example 4 is shown in Table 14 below.

[Table 14]

Experimental group Actual modulus of elasticity (R5) When using nutrition lotion 1 0.140 When using nourishing lotion 2 0.070 When using nourishing lotion 3 0.035 When using a control nutrition lotion 0.011

As can be seen from the results in Table 14 above, the actual modulus of elasticity was 0.140 when the nutrient lotion 1 containing the loquat extract (original invention) of Preparation Example 1 was used. The actual modulus of elasticity in the case of using the nutrient lotion 1 was 200% higher than that in the case of using the nutrient lotion 2 containing the loquat extract of Preparation Example 2, and 400 compared to the case of using the nutrient lotion 3 containing the loquat extract of Preparation Example 3 % Higher, 1272% higher than the control lotion containing no loquat extract. In conclusion, it was found that the nourishing lotion containing the loquat extract of the present invention effectively enhances skin elasticity.

Comparative Example 9 Safety of Nutritional Lotion

The test to determine whether the nourishing lotion 1 to nutrient lotion 3 prepared in Preparation Example 6 is safe for human skin. The results obtained by testing in the same manner as in Comparative Example 5 are shown in Table 15 below.

TABLE 15

Experimental group After 24 hours After 48 hours When using nutrition lotion 1 0/30 0/30 When using nourishing lotion 2 1/30 0/30 When using nourishing lotion 3 0/30 1/30

As can be seen from the results in Table 15, after 24 hours of application of the nourishing lotion 2, there was one subject who showed a positive response to skin irritation, erythema, itching, allergy, etc., but after 48 hours None of the subjects tested positive after the trouble subsided. In the case of the nutrient lotion 3, one subject showed a positive reaction to skin irritation, erythema, itching, allergy, etc. after 48 hours.

On the other hand, among the subjects who used the nourishing lotion 1 containing the loquat extract (invention of the present invention) of Preparation Example 1, those who showed a positive response to skin irritation, erythema, itching, allergy, etc. not only after 24 hours but also after 48 hours There was no one. From the above results, it was confirmed that the nutritional lotion containing the loquat extract of the present invention is very excellent in safety for human skin.

<Comparative Example 10>: Essence enhance skin elasticity effect

Essences 1 to 3 and the control essence prepared in Preparation Example 7 was tested to determine whether there is an effect to enhance skin elasticity. Actual elastic modulus (R5) obtained by the same method as in Comparative Example 4 was shown in Table 16 below.

[Table 16]

Experimental group Actual modulus of elasticity (R5) When using essence 1 0.161 When using essence 2 0.075 When using essence 3 0.041 When using control essence 0.012

As can be seen from the results in Table 16, the actual modulus of elasticity was 0.161 when the essence 1 containing the loquat extract (original invention) of Preparation Example 1 was used. The actual modulus of elasticity when the essence 1 was used was 214.67% higher than that of the essence 2 containing the loquat extract of Preparation Example 2, and 392.68% higher than that of the essence 3 containing the loquat extract of Preparation Example 3. In conclusion, it can be seen that the essence containing loquat extract of the present invention effectively enhances skin elasticity.

Comparative Example 11 Safety of Essence

Essences 1 to 3 prepared in Preparation Example 7 was tested to determine whether it is safe for human skin. The results obtained by testing in the same manner as in Comparative Example 5 are shown in Table 17 below.

TABLE 17

Experimental group After 24 hours After 48 hours When using essence 1 0/30 0/30 When using essence 2 1/30 0/30 When using essence 3 0/30 1/30

As can be seen from the results in Table 17, after 24 hours after the application of Essence 2, there was one subject who showed a positive reaction to skin irritation, erythema, itching, allergy, etc., but after 48 hours None of the subjects were calm and positive. In the case of essence 3, there was one subject who showed a positive response to skin irritation, erythema, itching, allergy, and the like after 48 hours.

On the other hand, none of the subjects who used the essence 1 containing the loquat extract (the present invention) of Preparation Example 1 had positive reactions against skin irritation, erythema, itching, and allergies not only after 24 hours but also after 48 hours. There was no. From the above results, it was confirmed that the essence containing loquat extract of the present invention, the safety for human skin is very excellent.

The present invention has been described above in connection with specific embodiments of the present invention, but this is only an example and the present invention is not limited thereto. Those skilled in the art can change or modify the described embodiments without departing from the scope of the present invention, and such changes or modifications are within the scope of the present invention. In addition, the materials of each component described herein can be readily selected and substituted for various materials known to those skilled in the art. Those skilled in the art will also appreciate that some of the components described herein can be omitted without degrading performance or adding components to improve performance. In addition, those skilled in the art may change the order of the method steps described herein depending on the process environment or equipment. Therefore, the scope of the present invention should be determined by the appended claims and equivalents thereof, not by the embodiments described.

Claims (4)

delete delete delete Ebibotrya japonica Seed extract 8.00wt%, methylparaben 0.15wt%, hyaluronic acid extract 2.50wt% Essence consisting of 8.00 wt% glycerin, 0.10 wt% propylparaben, 0.60 wt% lecithin, 10.0 wt% macadamia nut oil, 0.07 wt% fragrance and 70.58 wt% purified water.