KR101168567B1 - Composition and Method for Controling Post-harvest Disease of Citrus Fruit or Sweet Potato - Google Patents

Abstract

The present invention provides a method of controlling citrus or sweet potato reservoir control composition and control method of citrus or sweet potato storage bottles using Anagalarosaponin IV, Anagalarosaponin Ⅷ or Anagallysin C isolated from Shrimp Shrimp Extract It starts. The Sagefish Extract, Anagalarosaponin IV, Anagallosaponin VII and Anagallycin C are all antifungal against penicillium digititatum and / or penicillium italicom, which are the main pathogens of citrus or sweet potato storage diseases. It is active.

Description

Composition and Method for Controlling Post-harvest Disease of Citrus Fruit or Sweet Potato

The present invention relates to a tangerine or sweet potato reservoir control composition and a method of controlling tangerine or sweet potato reservoir bottles.

The biggest problem during distribution and storage after citrus harvesting is the citrus storage bottle, which is corrupted by the penicillium spp. ( Picicillium spp.), Especially during storage, which causes severe damage.

Penicillin-based gyundeul causing citrus storage disease, the cause of green mold disease on citrus Penny Room Solarium digital Tatum (P. digitatum), causing the blue mildew Penny Room Solarium Italy Com (P. italicum), and Penny chamber causing whisker mold P. ulaiense has been reported, most of which is caused by Penicillium digitatum and P. italicum Brown & Eckert, Compendium of Citrus Diseases 2nd, pp. 41-42, 2000; Holmes et al., Phytopathology , 84, pp719-727, 1994).

In order to control them, various methods including drug control have been developed. In particular, drug control using Imazalil is widely used in the United States (Eckert et al., Plant Disease, 78, pp971 to 974, 1994; Schirra). Et al., Proc. Int. Soc. Citriculture, pp 1060-1063, 1992; Smilanick et al., Plant Disease, 81, pp 379-382, 1997). Further biological using a number of micro-organisms in addition to yeast is Candida oleo pillar of microbial pesticides using (Candida oleophila) and the bacterium Pseudomonas ache dog (Pseudomonas syringae) is already commercialized, and is commercially available in the Aspire ^TM and Biosave ^TM is the tradename, respectively, Control has been attempted (Droby et al., Biological Control , 12, pp 97-101, 1998; Arras, Postharvest Biology and Technology , 8, pp 191-198, 1996).

On the other hand, when penicillium italicum stores sweet potatoes at 16 ° C or less, it invades other pathogens when the sweet potato tissue is sick or injured, causing sweet potato storage disease (also called sweet potato blue mold disease).

The sweet tissue of sweet potatoes is rather hard, dry, slightly moist, and brown. It is characterized by the growth of blue mold on the diseased tissue, and the appearance of white mycelium masses. Eventually, the blue mold packaging mass forms on the hyphae.

There is no effective control method for sweet potato storage bottles. However, methods are proposed to manage them carefully so as not to injure them during storage and to keep the temperature below 16 ° C.

The present invention is an anti-fungal activity in penicillium digittatum or penicillium italicomb that causes citrus or sweet potato storage disease ( Lysimachia) mauritiana Lamack) discloses an active substance isolated from the extract and the extract.

It is an object of the present invention to provide a citrus or sweet potato storage bottle control composition using the magpie swimming extract and the active material isolated from the extract.

Another object of the present invention is to provide a method for controlling citrus or sweet potato storage bottles using the composition.

The present invention is a penicillium digi which is the main pathogen of citrus or sweet potato storage bottles, which is 80% ethanol extract of the magnolia swim (outpost), fractions such as butanol of the extract, and three active substances isolated from the fractions. It was completed by confirming that it has antifungal activity against tatum and / or penicillium italicum.

The three active substances Ⅳ saponin to get ah, respectively (Anagallosaponin Ⅳ), ah out as saponin Ⅷ (Anagallosaponin Ⅷ) and O out of the lysine C, and all materials known as (Anagallisin C) (Chem Pharm Bull . 42 (9): 1750-5, 1994; Phytochemistry . 37 (5): 1397-402, 1994; Journal of Asian Natural Products Research , 10, 265-270, 2008; Journal of Natural Products , 58 (10): 1492-1497, 1995; CAS No.:162763-02-2 (Anagallosaponin IV); CAS No .: 160632-43-9 (Anagallosaponin VII), CAS No .: 114318-84-2 (Anagallisin C)), and its chemical formula can be found in the following [Formulas 1 to 3]. The three substances are separated from the magnolia swim or are known to contain these substances according to the methods set forth in the Examples below. arvensis L.) (Noburu et al ., Chem Pharm Bull . 42 (9): 1750-5, 1994), Lysimachia , Magpie christinae ) (Tian et al., Journal of Asian Natural Products Research, 10, 265-270, 2008) , myrsinaceae plants in Mir ahwooseu new lease Tralee (Myrsine australis ) (Hegde et al., Journal of Natural Products , 58 (10): 1492-1497, 1995) and the like. All documents cited above are considered to be part of this specification.

(Formula 1)

Anagalrosaponin Ⅳ

(Formula 2)

Anagalrosaponin Ⅷ

(Formula 3)

Anagallysin C

In view of the foregoing, the citrus or sweet potato reservoir control composition of the present invention is a magnolia swimming extract, a fraction of the magnolia swimming extract, anagalarosaponin IV, angalalosaponin Ⅷ or ananaglycine C as an active ingredient. It is characterized by including.

In the present specification, the term "active ingredient" means a component that can exhibit activity alone or in combination with a carrier which is not active in itself.

In addition, in the present specification, "scatfish swimming extract" refers to the extracts, stems, leaves, roots, fruits, seeds and / or flowers of the magpie swimming, which are to be extracted regardless of the extraction method, such as methanol, ethanol, butanol, etc. It means an extract obtained by extraction with a lower alcohol having 1 to 5 carbon atoms, acetone, hexane, ethyl acetate, dichloromethane, water or a mixed solvent thereof. Regardless of the extraction method, it is to be understood that the extraction method may be applied to any method such as cooling, refluxing, heating, ultrasonic radiation, and the like, as long as the extraction object is extracted through immersion in the extraction solvent. Nevertheless, the "squash swim extract" preferably means an extract obtained by extracting with water, ethanol or a mixed solvent thereof, more preferably a mixed solvent of water and ethanol, in particular 70-90% of ethanol. In% means volume%, which is the same below). The extract may be filtered, concentrated and the phase may be liquid or powder.

In addition, in the present specification, "a fraction of magpie swimming extract" refers to a magnolia swimming extract, preferably a mixed solvent of water and ethanol, in particular 70 to 90% ethanol of a magpie swimming extract obtained from a mixed solvent of methanol and water. Suspended in ˜40% methanol, and then fractionated in any solvent layer obtained by fractionation with butanol or hexane, and further, the magpie swimming extract is suspended in a mixed solvent of methanol and water, in particular 10-40% methanol, Means an extract obtained by sequentially dividing with dichloromethane, ethyl acetate and butanol. By "sequentially fractionating" here is meant that fractions of water and methanol layer continue to be used after fractionation with solvents in the order listed above.

Also herein, "control" means the prevention, treatment or alleviation of symptoms of citrus or sweet potato storage disease.

In addition, in the present specification, "citrus" means a citrus ( C. natsudaidai Hayata), citron grown in southern Europe, lemon (lemon) grown in California, the United States, paragraph or grapefruit grown in the tropics, etc. Paragraphs, such as grape fruit grown in the US, Floridi, etc., grape fruit, sour oranges grown in India, Italy, etc. It includes all the citrons grown in Korea, tangerines grown in Jeju Island, Korea, gold persimmons grown in China, and other tangerines.

The active ingredient in the control composition of the present invention is in the range of 1 to 99% by weight based on the total weight of the control composition of the present invention according to its formulation, its use time (right after harvest, immediately after harvest, etc.), and the like. It may be included in any amount, it will usually be included in the range of 1 to 30% by weight, usually in the liquid formulation will be included in the range of 10 to 20% by weight, in powder powder usually 30 to 40% by weight.

The control composition of the present invention may be prepared as a formulation of the active ingredients, or may be mixed with an agriculturally acceptable carrier to be formulated into an emulsion, solution, suspension, hydrating agent, flow agent, powder, granule, dispersant, and the like.

In the above, the "agrochemically acceptable carrier" is combined with the active ingredient of the control agent composition of the present invention to promote the spreading, storage and / or transport of the active ingredient without inhibiting the activity of the active ingredient and to plant or human body. Any substance that does not exhibit acceptable toxicity.

Carriers that can be used are soil natural minerals (e.g. kaolin, clay, talc, chalk), soil synthetic minerals (e.g. highly dispersed silica, silicate), carbon based materials (charcoal, bitumen, etc.), sulfur, natural or synthetic Resins, fertilizers, cellulose based materials (sawdust and corncobs), water, aromatic solvents (e.g. Solvesso products, xylene), paraffins (e.g. mineral oil fractions), alcohols (e.g. methanol, butanol, pentanol, Benzyl alcohol), ketones (e.g. cyclohexanone, methyl hydroxybutyl ketone, diacetone alcohol, mesityl oxide, isophorone), lactones (e.g. gamma-butyrolactone), pyrrolidone (pyrrolidone) , N-methylpyrrolidone, N-ethylpyrrolidone, n-octylpyrrolidone), acetates (glycol diacetate), glycols, dimethyl fatty acid amides, fatty acids, fatty acid esters, and mixtures thereof.

The control composition of the present invention may also include one or more adjuvants such as wetting agents, dispersants, antifreezes, thickeners, preservatives, electrodeposition agents.

Such adjuvants may be selected and used appropriately from those known in the art, for example, alkylnaphthalene sulfonate (particularly diisopropylnaphthalenesulfonate or diisobutylnaphthalenesulfonate) may be used as the wetting agent, and nonionic as a dispersant. Dispersants (ethylene oxide / propylene oxide block polymers, alkylphenol polyglycol ethers, tristyrylphenol polyglycols, etc.), anionic dispersants (ligninsulfonic acid, naphthalenesulfonic acid, phenolsulfonic acid, dibutylnaphthalenesulfonic acid, alkylarylsulfonates, alkyl sulfides) Pate, alkylsulfonate, etc.) or mixtures thereof, methanol, ethanol, isopropanol, butanol, glycol, glycerin, diethylene glycol and the like can be used, and as a thickener, cellulose derivatives, polyacrylic acid derivatives, xanthan Modified clay As the preservative, dichlorophene, isothiazolene, benzyl alcohol, etc. can be used, and the electrodeposition agent is polyvinyl alcohol, polyvinylpyrrolidone, polyacrylate, polymethacrylate, polybutene, polyisobutylene , Polystyrene, polyethyleneamine, polyethyleneamide, polyethyleneimine, polyether and the like can be used.

In another aspect, the present invention relates to a method for controlling citrus or sweet potato storage bottles using the control composition of the present invention as described above.

The method for controlling citrus or sweet potato storage bottles of the present invention is characterized by comprising the step of contacting the control composition of the present invention with citrus.

The contacting step will typically be accomplished by spraying directly on the citrus or sweet potato after harvest or in circulation, but may also be applied by spraying just before harvest or by fumigation of the citrus or sweet potato cellar.

As described above, according to the present invention, it is possible to provide a citrus or sweet potato storage bottle control composition using the magpie swimming extract and the active substance isolated from the extract and a tangerine or sweet potato storage bottle control composition using the composition. .

Figure 1 shows the results of antifungal activity evaluation of the 80% ethanol extract of Shrike.
Figure 2 is an evaluation of the antifungal activity of each fraction of the magpies.
Figure 3 shows the antifungal activity of the fourth, seventh and eighth purified tablets obtained by performing reversed phase silica gel column chromatography and normal phase silica gel column chromatography in butanol fractions.
Figure 4 is a schematic diagram of the separation process of the active material.
FIG. 5 is the 1 H NMR spectrum of Purification No. 4 (Angalalosaponin IV). FIG.
FIG. 6 is a 13C NMR spectrum and a DEPT spectrum of Purification No. 4 (Anagalosaponin IV). FIG.
FIG. 7 is an LC-MS / MS spectrum of Purification No. 4 (Anagalosaponin IV). FIG.
FIG. 8 is a 1 H NMR spectrum of Purification No. 7 (Anagalosaponin VII). FIG.
Figure 9 is a 13C NMR spectrum of Purification No. 7 (Analogalasponin VII).
FIG. 10 is an LC-MS / MS spectrum of Purification No. 7 (Anagalosaponin VII). FIG.
FIG. 11 is the 1H NMR spectrum of the eighth purified product (anagallysin C).
Figure 12 is a 13C NMR of Purification No. 8 (Anagalizin C).
FIG. 13 is an LC-MS / MS spectrum of Purification No. 8 (anagallysin C). FIG.

Hereinafter, the present invention will be described with reference to Examples and Experimental Examples. However, the scope of the present invention is not limited to these examples and experimental examples.

<Example 1> Preparation of swimming Conger blackcurrant extract and fractions

1 kg of fine valued swimming (outpost) was added to 80% ethanol, immersed for 48 hours, extracted three times, and the extract was filtered under reduced pressure to remove the solvent and freeze-dried to give 275 g (27.5%) of a powdery extract. .

Then, 275 g of the obtained ethanol extract was suspended and dissolved in 100-fold 20% methanol, and then sequentially partitioned into hexane (n-hexane), dichloromethane (CH ₂ Cl ₂ ), ethyl acetate (EtOAc) and butanol (BuOH). 8.35 g (3.0%) in hexane layer, 9.01 g (3.3%) in dichloromethane layer, 18.72 g (6.8%) in ethyl acetate layer, 82.42 g (29.9%) in butanol layer and 115.03 g (41.8%) in residual water layer Fractions).

<Example 2> Shrimp Swallow extract and fractions Evaluation of antifungal activity against green mold disease ( P. digitatum ) and blue mold disease ( P. italicum )

<Example 2-1> Conger blackcurrant swimming evaluate antifungal activity of 80% EtOH extract

In order to evaluate the mycelial formation inhibitory ability of myrtle swimming extract, fungal mycelium with a diameter of 6 mm at the edges of mycelium after 5 days of incubation on potato agar medium (PDA, potato dextrose agar) Sections were collected and inoculated in potato agar medium in which the magnolia swim extracts were added at a concentration of 2 mg / mL, 1 mg / mL, and 0.5 mg / mL, and cultured at 27 ° C. for 30 days to determine the production of the flora. The results are shown in Figure 1, the larvae extract did not grow mycelia at all, the same as the chemical pesticides (brand name: Befran) used as a positive control in green fungal disease, the mycelia growth to some extent in blue fungal disease However, the inhibitory effect was more than 90% compared to the 80% EtOH treatment group which was used as an untreated group or an extraction solvent as a negative control (1, (-) control; 2, 80% EtOH; 3, (+) control). 4, 2 mg / mL extract; 5, 1 mg / mL extract; 6, 2 mg / mL extract).

<Example 2-2> Conger Magpie swimming Fraction Antifungal power rating

In order to evaluate the mycelial formation inhibitory ability of anti-fungal fractions in the waters of S. oleracea extract, green fungal disease and blue mold disease fungi were cultured on potato agar medium (PDA) for 5 days, and then the diameters at the edges of the floras. Mycelial slices of 6 mm were collected, and 40 μL of the large-sized Swallow fractions of 50 mg / mL were dropped on a 6 mm diameter paper disk, inoculated in potato agar medium, and cultured at 27 ° C. for 10 days to measure the production of mycelia. The results are shown in Figure 2, the butanol fraction of the magnolia swimming fraction showed the best antifungal activity in both green and blue mold disease (1 and 5, (-) control; 2, water fraction; 3, 80% ethanol extract; 4, butanol fraction; 6, ethyl acetate fraction; 7, dichloromethane fraction; 8, hexane fraction).

<Example 3> Slaughterhouse Swimming Separation and Purification of Active Ingredients from Fractions and Their Purified Products Evaluation of Mycelial Growth Inhibitory Effect against Green Fungal Diseases ( P. digitatum ) and Blue Mold Diseases ( P. italicum )

After confirming the antifungal power of the 80% ethanol extract of the larvae swimming, 12 g of butanol fraction was subjected to column chromatography using reverse phase silica gel to obtain a total of 10 purified products. The column size was 7 cm × 20 cm and 20, 40, 60, 80, 100% methanol was used as the mobile phase. When the antifungal activity was evaluated for the 10 tablets by the same method as in <Example 2-2>, the antifungal activity of the 9th (GB-9) purified product was excellent and 1.1g of the 9th purified product Normal silica gel column chromatography was performed. The column size was 2.5 cm × 50 cm and chloroform: methanol (1: 1, v / v) was used as the mobile phase. The mobile phase was flowed at a rate of 1.5 ml per minute and a total of eight tablets were obtained.

Similarly, the antifungal activity was evaluated in the same manner as in <Example 2-2> with respect to the total eight purified products. ) And 8 (GB-9-8, 114 mg) tablets showed the highest activity. Figure 3 shows the results of the antifungal activity test of the 4th, 7th and 8th purification.

When the 4th, 7th and 8th purifications were developed on TLC (silica gel 60 F 254, chloroform; methanol = 1: 1, v / v), the Rf was 0.6, 0.3, 0.28 and showed a single spot, respectively. 4 is a schematic diagram showing the separation process of the active material.

Example 4 Identification of Active Material

Example 4-1 Identification of the Active Substance in Purified Product No. 4 (GB-9-4) -Anagalrosaponin IV

In order to analyze the structure of the isolated active material, 20 mg of purified No. 4 was dissolved in methanol-d4 and the NMR spectrum was measured. Eight methyl groups were identified in the 1 H NMR spectrum (FIG. 5), and one of them was identified as a methyl group (δ 2.1 ppm, s) connected to an acetyl group. In addition, it was expected that the glycoside form was determined from signals around 5-3.0 ppm. 13C NMR spectrum (Fig. 6) was confirmed from the carbonyl carbon of the acetyl group 172.6ppm 107.3, 105.6, 104.8, and the expected that attached to the glycoside per delayed four into four anomeric carbon at 104.3 per literature (Phytotherapy research , 12, s70-s73, 1998) and 13-C NMR chemical shift. -O- [β-D-xylopyranosyl- (1 → 2) -O-β-D-glucopyranosyl- (1 → 4)]- It was confirmed that the glycoside in the form of [O-β-D-glucopyranosyl- (1 → 2)]-O-α-L-arabinopyranosyl. In addition, it was confirmed that 8 quaternary carbons, 24 CH, 14 CH ₂ , and 8 CH ₃ in the DEPT spectrum (FIG. 6) showed that the total carbon number of the compound was 54. Excluding the carbon number per 4 from the total carbon number of the compound, the structure of the aglycone having 32 carbon atoms was expected to be a triterpenoid form composed of 5-rings bound by acetyl groups. In addition, the 77.5ppm signal, CH ₂ , was moved to the low magnetic field, indicating that the epoxy was bound to oxygen in the ring. Therefore, as a result of searching the literature ( Chem . Phram . Bull . 42 (9) 1750-1755, 1994), the structure of the active substance was identified as Anagalarosponin IV of [Formula 1].

LC-MS / MS analysis was performed to determine the molecular weight of the identified material. Anagallosaponin IV had a molecular weight of 1104.57 and was analyzed to be 1128.19 in sodium-bound form by LC-MS / MS analysis. As a result of performing MS / MS for more accurate structure confirmation, it was possible to obtain Anagallosaponin IV-specific cleavage patterns as shown in FIG. 7.

Example 4-2 Identification of the Active Substance in Purified Product No. 7 (GB-9-7) -Anagalrosaponin Ⅷ

In order to analyze the structure of the separated active material, 20 mg of purified No. 7 was dissolved in methanol-d4 and NMR spectrum was measured. Seven methyl groups were identified in the 1 H NMR spectrum (FIG. 8), and one of them was identified as a methyl group (δ 2.1 ppm, s) connected to an acetyl group. In addition, it was expected that the glycoside form was determined from signals around 5-3.0 ppm. 13C NMR spectra (Figure 9) were confirmed at the carbonyl carbon of the acetyl group 172.6ppm 107.4, 104.8, 104.8, and the expected that attached to the glycoside per delayed four into four anomeric carbon at 104.3 per literature (Phytotherapy research , 12, s70-s73, 1998) and 13-C NMR chemical shift. -O- [β-D-xylopyranosyl- (1 → 2) -O-β-D-glucopyranosyl- (1 → 4)]- It was confirmed that the glycoside in the form of [O-β-D-glucopyranosyl- (1 → 2)]-O-α-L-arabinopyranosyl. 13C NMR spectra predicted that the total carbon number of the compound was 54, and the structure of the aglycone with 32 carbon atoms, excluding the carbon number per 4 from the total carbon of the compound, was expected to be in the form of tirterpenoids composed of five rings bound by acetyl groups. It was. Unlike Anagallosaponin IV, 13OH 28.3ppm signal disappeared and 64.6ppm signal appeared, suggesting that OH was bound to methyl group 23. In addition, the signal of 77.5ppm showed that the epoxy form. Therefore, as a result of searching the literature ( Chem . Phram . Bull . 42 (9) 1750-1755, 1994), the structure of the active substance was identified as Anagalarosponin 의 of [Formula 2].

LC-MS / MS analysis was performed to determine the molecular weight of the identified material. Anagalarosponin VIII had a molecular weight of 1120.57 and was analyzed to be 1144.05 in sodium-bound form by LC-MS / MS analysis. As a result of performing MS / MS to confirm the structure more accurately, it was possible to obtain the cleavage pattern inherent in anagalaroponin Ⅷ as shown in FIG. 10.

Example 4-3 Identification of the Active Substances in Purification No. 8 (GB-9-8) -Anagallysin C

In order to analyze the structure of the isolated active material, 20 mg of purified No. 8 was dissolved in methanol-d4 and NMR spectrum was measured. Six methyl groups were identified in the 1 H NMR spectrum (FIG. 11). In addition, it was expected that the glycoside form was determined from signals around 5-3.0 ppm. In addition, unlike the compounds 4 and 7 identified above, no carbonyl carbon of the acetyl group was observed in the 13C NMR spectrum (FIG. 12), and 4 sugars were obtained from 4 anomer carbons per 107.3, 104.7, 104.7, and 104.2. In comparison with the expected glycosides ( Phytotherapy research , 12, s70-s73, 1998) and the 13C NMR chemical shift, -O- [β-D-xylopyranosyl- (1 → 2) -O-β It was confirmed that the glycoside in the form of -D-glucopyranosyl- (1 → 4)]-[O-β-D-glucopyranosyl- (1 → 2)]-O-α-L-arabinopyranosyl. 13 NMR showed that the total carbon number of the compound was 52. When the total carbon number of the compound was excluded, the structure of the aglycone having 30 carbon atoms was expected to be in tirterpenoid form. And 64.5ppm signal was confirmed in the same manner as the single compound 7 and the methyl group 23 was confirmed that the OH is bound form, it can be seen that the epoxy form of the 77.5ppm signal. Therefore, as a result of searching the literature ( Chem . Phram . Bull . 42 (9) 1750-1755, 1994), the structure of the active substance was identified as anagalicin C.

LC-MS / MS analysis was performed to determine the molecular weight of the identified material. Anagallysin C had a molecular weight of 1062.23 and was analyzed to be 1086.13 in sodium-bound form by LC-MS / MS analysis. As a result of performing MS / MS to confirm the structure more accurately, it was possible to obtain the cleavage pattern peculiar to analagasin C as shown in FIG. 13.

Claims (7)

An active ingredient comprising one of the following (I) to (V) showing antimicrobial activity against penicillium digitatum and penicillium italicum , the causative agents of citrus or sweet potato storage diseases Citrus or sweet potato reservoir control composition.
(I) magpie swimming extract,
(Ⅱ) fractions of the magnolia swimming extract,
(III) anagalarosponin Ⅳ,
(IV) anagalarosponin shock and
(Ⅴ) Anagallysin C
The method of claim 1,
The extract is citrus or sweet potato storage bottle control composition, characterized in that obtained by dipping magpie swimming in a mixed solvent of water and ethanol.
The method of claim 1,
The fraction is a citrus or sweet potato storage bottle control composition, characterized in that one of the following (I) and (II)
(I) a fraction of any solvent layer obtained by suspending magnolia swimming in a mixed solvent of water and ethanol, suspended in a mixed solvent of methanol and water, and then fractionating with butanol or hexane; And
(II) Hexane layer or butanol obtained when the extract obtained by dipping magpie swimming in a mixed solvent of water and ethanol is suspended in a mixed solvent of methanol and water, and then sequentially fractionated using hexane, dichloromethane, ethyl acetate and butanol. Fractions of layers.
The method of claim 2,
The mixed solvent of ethanol and water is citrus or sweet potato storage bottle composition, characterized in that 70 to 90% ethanol.
The method of claim 3,
The mixed solvent of ethanol and water is 70 to 90% ethanol, the mixed solvent of methanol and water is 10 to 40% methanol, citrus or sweet potato storage bottle control composition.
The method of claim 1,
The composition of claim 1, wherein the composition further comprises an agriculturally acceptable carrier and at least one auxiliary agent selected from the group consisting of wetting agents, dispersants, antifreeze agents, thickeners, preservatives and electrodeposition agents.
A method of controlling citrus or sweet potato storage bottles comprising the step of contacting the citrus or sweet potato reservoir control composition according to any one of claims 1 to 6 with the tangerine or sweet potato.

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