KR101168566B1 - Compostion and Method for Controling Plant Disease Occurred by Magnaporthe grisea - Google Patents

Abstract

The present invention relates to a plant disease control composition and magnaporte griesae by Magnaporte grisea using Anagala saponin Ⅳ, Anagala saponin Ⅷ, or Anagalisin C isolated from the magpie swimming extract. A plant disease control method by the same is disclosed. The larvae extract, Anagalarosaponin IV, Anagalarosaponin VII, and Desglucoalogalside B all exhibit antifungal activity against Magnaporte greece.

Description

Composition and Method for Controlling Plant Disease Occurred by Magnaporthe grisea

The present invention relates to a plant disease control composition and a plant disease control method by Magna Porte Grisse.

Magnaporthe grisea is a causative agent of fungal diseases and belongs to the fungal follicles. The pathogens overwinter in the form of conidia and then scatter and form a lesion by attaching and invading the host. Optimal growth temperature of the mycelia is 27 ~ 28 ℃.

Magnaporthe grisea ) (the name of the incomplete generation is Pyricularia grisea ) belongs to the asymptomatic fungus of the fungal system, and mainly exists as conidia. The colorless conidia are pear-shaped and have two diaphragms. Optimal mycelial growth temperature for pathogens is 27-28 ° C. The time required for intrusion is about 6 hours at 24 ° C and 8 hours at 20 ° C and 28 ° C.

The pathogens overwinter in the form of conidia and then scatter and form a lesion by attaching and invading the host. When there are frequent rains and lack of sunshine, cold and humid weather continues, when the soil temperature is low, the soil moisture content is low, and when nitrogen fertilizer is overused, the planting is poor when the planting is poorly ventilated and the rice transplanting season is late. It is a good condition.

The host plants of Magna Forte Grissea are rice plants such as rice, grass, wheat, barley, and blood.

Rice blast is a disease that causes great damage at all stages of growth from the early to late stages of rice production in the world where rice is grown (Leung & Taga, Advances in Pathology Genetic of Plant Pathogenic Fungi , pp. 175-). 188, 1988). The disease occurs under conditions of frequent rain and insolation, and according to the damage area, it is divided into leaf blast, ear blast, ear branch blast, rice blast, node blast, and leaf blast. The cases of young mothers are called fever.

Turf blast is mainly caused by St. Augustinegrass, Kentucky bluegrass, Perennial ryegrass, and tall fescue, and sometimes occurs in Korean grass. It is a kind. Round or oval, pale gray lesions formed on the leaves or stems of grass, and these lesions overlap, causing the entire leaves to brown and die. In 18 samples of 6 golf courses in Jeju Island, 25.87% of the diseases were found. Dallas Spot 20.69%. Turf fever was found in 19.28% (Jeju Environment Daily December 14, 2009 article).

Methods to reduce the blast damage caused by Magna Forte Grisse include the use of resistant varieties, fertilization control, seedling control such as vegetarian density control, chemical control using chemical pesticides such as tricyclazol and Capropamid, and microbial culture such as actinomycetes. Patent No. 2006-0019866), extracts such as pomegranate peel or leek seed ( The Korean Journal of Mycology , 33 (2): 86-88), rate control (Korean Patent Publication No. 2006-0039954), etc. It can be divided into.

The present invention also provides a biological control method.

An object of the present invention is Conger blackcurrant swimming (Lysimachia mauritiana Lamack) to provide a plant disease control agent composition according to Magna Forte so years old child with active substance isolated from the extract and the extract.

Another object of the present invention is to provide a method for controlling a plant disease by Magna Porte Grisse using the composition.

The present invention is directed against 80% ethanol extracts of magnolias (outposts), fractions such as butanol and the like, and three active substances isolated from the fractions against magnaporte grisea, a pathogen such as rice blast. It is completed by confirming having fungal activity.

The three active substances are anagallosaponin IV and angallosaponin IV, respectively.(Anagallosaponin iii) and Anagallisin C, both known substances (Chem Pharm Bull . 42 (9): 1750-5, 1994;Phytochemistry . 37 (5): 1397-402, 1994;Journal of Asian Natural Products Research, 10, 265-270, 2008;Journal of Natural Products58 (10): 1492-1497, 1995; CAS No.:162763-02-2 (Anagallosaponin IV); CAS No .: 160632-43-9 (Anagallosaponin VII), CAS No .: 114318-84-2 (Anagallisin C)), and the chemical formula thereof can be found in the following [Formulas 1 to 3]. The three materials are separated from the magpie swimming, or are known to contain these materials according to the methods set forth in the Examples below.Anagallis arvensis L.) (Noburu et al.,Chem Pharm Bull . 42 (9): 1750-5, 1994), Rishimacia CristinaLysimachia christinae) (Tian et al., Journal of Asian Natural Products Research, 10, 265-270, 2008), Myrtle Australis, the Forbidden Plant (Myrsine australis(Hegde et al.,Journal of Natural Products, 58 (10): 1492-1497, 1995) and the like. All documents cited above are considered to be part of this specification.

(Formula 1)

Anagalrosaponin Ⅳ

(Formula 2)

Anagalrosaponin Ⅷ

(Formula 3)

Anagallysin C

In view of the foregoing, the control agent composition of the present invention is characterized by comprising a magpie swimming extract, a fraction of a magpie swimming extract, an gallo saponin IV, an gallo saponin Ⅷ or an agalagasin C as an active ingredient. do.

In the present specification, the term "active ingredient" means a component that can exhibit activity alone or in combination with a carrier which is not active in itself.

In addition, in the present specification, "scatfish swimming extract" refers to the extracts, stems, leaves, roots, fruits, seeds and / or flowers of the magpie swimming, which are to be extracted regardless of the extraction method, such as methanol, ethanol, butanol, etc. It means an extract obtained by extraction with a lower alcohol having 1 to 5 carbon atoms, acetone, hexane, ethyl acetate, dichloromethane, water or a mixed solvent thereof. Regardless of the extraction method, it is to be understood that the extraction method may be applied to any method such as cooling, refluxing, heating, ultrasonic radiation, and the like, as long as the extraction object is extracted through immersion in the extraction solvent. Nevertheless, the "squash swim extract" preferably means an extract obtained by extracting with water, ethanol or a mixed solvent thereof, more preferably a mixed solvent of water and ethanol, in particular 70-90% of ethanol. In% means volume%, which is the same below). The extract may be filtered, concentrated and the phase may be liquid or powder.

In addition, in the present specification, "a fraction of magpie swimming extract" refers to a magnolia swimming extract, preferably a mixed solvent of water and ethanol, in particular a magpie swimming extract obtained from 70 to 90% ethanol, in particular a mixed solvent of methanol and water Suspended in 10-40% methanol, which means a fraction of a solvent layer obtained by fractionation with butanol or hexane, and further, after the suspension of the magpie swimming extract is suspended in a mixed solvent of methanol and water, in particular 10-40% methanol, Means an extract obtained by sequentially fractionating with hexane, dichloromethane, ethyl acetate and butanol. By "sequentially fractionating" here is meant that fractions of water and methanol layer continue to be used after fractionation with solvents in the order listed above.

In addition, in this specification, "control" means the prevention, treatment or alleviation of symptoms of plant diseases caused by Magna Porte Grisse.

In addition, in the present specification, "plant diseases caused by Magna Forte Grisse" means a rice blast and grass blast, and barley, wheat, blood and the like are also host plants of Magna Porte grisea, meaning that they also include blasts of these plants. .

The control agent composition of the present invention may be included in any amount within the range of 1 to 99% by weight of the active ingredients, depending on its formulation, the subject to be applied, etc., based on the total weight of the control agent composition of the present invention, It will typically be included in the range of 1 to 30% by weight, usually in the range of 10 to 20% by weight in liquids, and typically in the range of 30 to 40% by weight in powders.

The control composition of the present invention may be prepared as a formulation of the active ingredients, or may be mixed with an agriculturally acceptable carrier to be formulated into an emulsion, solution, suspension, hydrating agent, flow agent, powder, granule, dispersant, and the like.

In the above, the "agrochemically acceptable carrier" is combined with the active ingredient of the control agent composition of the present invention to promote the spreading, storage and / or transport of the active ingredient without inhibiting the activity of the active ingredient and acceptable to plants It refers to any substance that does not exhibit the above toxicity.

Carriers that can be used are soil natural minerals (e.g. kaolin, clay, talc, chalk), soil synthetic minerals (e.g. highly dispersed silica, silicate), carbon based materials (charcoal, bitumen, etc.), sulfur, natural or synthetic Resins, fertilizers, cellulose based materials (sawdust and corncobs), water, aromatic solvents (e.g. Solvesso products, xylene), paraffins (e.g. mineral oil fractions), alcohols (e.g. methanol, butanol, pentanol, Benzyl alcohol), ketones (e.g. cyclohexanone, methyl hydroxybutyl ketone, diacetone alcohol, mesityl oxide, isophorone), lactones (e.g. gamma-butyrolactone), pyrrolidone (pyrrolidone) , N-methylpyrrolidone, N-ethylpyrrolidone, n-octylpyrrolidone), acetates (glycol diacetate), glycols, dimethyl fatty acid amides, fatty acids, fatty acid esters, and mixtures thereof.

The control composition of the present invention may also include one or more adjuvants such as wetting agents, dispersants, antifreezes, thickeners, preservatives, electrodeposition agents.

Such adjuvants may be selected and used appropriately from those known in the art, for example, alkylnaphthalene sulfonate (particularly diisopropylnaphthalenesulfonate or diisobutylnaphthalenesulfonate) may be used as the wetting agent, and nonionic as a dispersant. Dispersants (ethylene oxide / propylene oxide block polymers, alkylphenol polyglycol ethers, tristyrylphenol polyglycols, etc.), anionic dispersants (ligninsulfonic acid, naphthalenesulfonic acid, phenolsulfonic acid, dibutylnaphthalenesulfonic acid, alkylarylsulfonates, alkyl sulfides) Pate, alkylsulfonate, etc.) or mixtures thereof, methanol, ethanol, isopropanol, butanol, glycol, glycerin, diethylene glycol and the like can be used, and as a thickener, cellulose derivatives, polyacrylic acid derivatives, xanthan Modified clay As the preservative, dichlorophene, isothiazolene, benzyl alcohol, etc. can be used, and the electrodeposition agent is polyvinyl alcohol, polyvinylpyrrolidone, polyacrylate, polymethacrylate, polybutene, polyisobutylene , Polystyrene, polyethyleneamine, polyethyleneamide, polyethyleneimine, polyether and the like can be used.

The control composition of the present invention may further contain other known components such as herbicide components, insecticidal components, bactericidal components, etc., depending on the purpose of use, the environment of the arable land, and the like.

In another aspect, the present invention relates to a method for controlling plant diseases by Magna Porte Grisea using the control composition of the present invention as described above.

The method for controlling plant diseases by Magnaporte Grissea of the present invention is characterized by comprising the step of contacting the plant composition of the present invention, such as rice, grass, barley, wheat.

The contacting step will usually be achieved by spraying on the affected rice, grass, barley, wheat, or by spraying or incorporating into soil, which is the habitat environment of rice, grass, or the like, or by spraying the seeds or seedlings thereof It may also be achieved by spraying or mixing the growth medium or soil, which is the habitat environment for

Alternatively, the step of contacting may be made by mixing with a fungicide, insecticide, herbicide, fertilizer and the like.

The amount of the active ingredient of the present invention's control agent in plants such as rice and grass is comprehensively determined by the extent of growth of rice, grass, etc., degree of disease, soil conditions, rainfall, types of active ingredients to be used, and the like. Will be taken into account. Usually, 10 to 500 g, preferably 30 to 250 g per hectare, and 10 to 1000 g, preferably 100 to 500 g when spraying and mixing the soil.

As described above, according to the present invention, the plant disease control agent composition by Magna Porte Grissa using the magpie swimming extract and the active substance isolated from the extract and the plant disease control method by Magna porte Grissa using the composition Can be provided.

Figure 1 shows the results of antifungal activity evaluation of Magnaporte grisea of the magpies 80% ethanol extract.
FIG. 2 shows the results of evaluation of antifungal activity against Magnaporte griesea in each fraction of magpies.
Fig. 3 shows the results of antifungal activity test against Magna Porte Grisea from No. 4, No. 7, and No. 8 purified silica obtained by performing reverse phase silica gel column chromatography and normal phase silica gel column chromatography in butanol fractions.
Figure 4 is a schematic diagram of the separation process of the active material.
FIG. 5 is the 1 H NMR spectrum of Purification No. 4 (Angalalosaponin IV). FIG.
FIG. 6 is a 13C NMR spectrum and a DEPT spectrum of Purification No. 4 (Anagalosaponin IV). FIG.
FIG. 7 is an LC-MS / MS spectrum of Purification No. 4 (Anagalosaponin IV). FIG.
FIG. 8 is a 1 H NMR spectrum of Purification No. 7 (Anagalosaponin VII). FIG.
Figure 9 is a 13C NMR spectrum of Purification No. 7 (Analogalasponin VII).
FIG. 10 is an LC-MS / MS spectrum of Purification No. 7 (Anagalosaponin VII). FIG.
FIG. 11 is the 1H NMR spectrum of the eighth purified product (anagallysin C).
FIG. 12 is 13 C NMR of Purification No. 8 (Anagalizin C). FIG.
FIG. 13 is an LC-MS / MS spectrum of Purification No. 8 (anagallysin C). FIG.

Hereinafter, the present invention will be described with reference to Examples. However, the scope of the present invention is not limited to these examples.

<Example 1> Preparation of Conger swimming blackcurrant extract and fractions

1 kg of fine valued swimming (outpost) was added to 80% ethanol, immersed for 48 hours, extracted three times, and the extract was filtered under reduced pressure to remove the solvent and freeze-dried to give 275 g (27.5%) of a powdery extract. .

Then, 275 g of the obtained ethanol extract was suspended and dissolved in 100-fold 20% methanol, and then sequentially partitioned into hexane (n-hexane), dichloromethane (CH ₂ Cl ₂ ), ethyl acetate (EtOAc) and butanol (BuOH). 8.35 g (3.0%) in hexane layer, 9.01 g (3.3%) in dichloromethane layer, 18.72 g (6.8%) in ethyl acetate layer, 82.42 g (29.9%) in butanol layer and 115.03 g (41.8%) in residual water layer Fractions).

<Example 2> Conger blackcurrant Antifungal activity evaluation of the Magna Forte years old child so the rice blast fungi in swimming extracts and fractions

<Example 2-1> Conger blackcurrant swimming evaluate antifungal activity of the 80% ethanol extract

In order to evaluate the mycelial formation-inhibiting ability of fungi in 80% ethanol extracts, the rice bran fungus Magnaforte greece is cultured on potato agar medium (PDA) for 5 days and 6mm in diameter at the edges of the mycelia. Mycelial slices were harvested and inoculated on potato agar medium supplemented with 1 mg / mL of Shrike Swimmer extract and incubated at 27 ° C. for 30 days.

The results are shown in Figure 1, the mylar swimming extract did not grow mycelia at all until 15 days after inoculation, but began to form a little after 20 days, but more than 85% of the inhibitory effect was observed after 30 days.

<Example 2-2> Conger Magpie swimming Fraction Antifungal Activity Assessment

In order to evaluate the mycelial formation inhibitory ability against antifungal activity of fractions in Sagefish extract, M. grisea fungus was cultured for 5 days on potato dextrose agar (PDA) and 6mm in diameter at the edges of mycelia. Mycelial slices were collected and dropped into 40 μL of 50 mg / mL saponica swimming fractions, respectively, on 6 mm diameter paper disks, inoculated in potato agar medium, and cultured at 27 ° C. for 7 days to determine the production of mycelia.

The results are shown in Figure 2, but the hexane and butanol fractions of the Swallowtail fractions showed antifungal activity, but the superior antifungal activity was observed in the butanol fraction compared to 80% ethanol extract (1 and 5, (-) control). 2, water fraction; 3, 80% ethanol extract; 4, butanol fraction; 6, ethyl acetate fraction; 7, dichloromethane fraction; 8, hexane fraction).

<Example 3> Slaughterhouse Swimming Separation of the active ingredient fractions? Purification and the rice blast fungus Magna Forte Evaluation of antifungal activity against greece

12 g of butanol fraction showing the highest antifungal activity in <Example 2-2> was subjected to column chromatography using reverse phase silica gel to obtain a total of 10 purified products. The column size was 7 cm × 20 cm and 20, 40, 60, 80, 100% methanol was used as the mobile phase. When the antifungal activity of the 10 purified products was evaluated in the same manner as in <Example 2-2> (please confirm) , 9 (GB-9) of the purified products had excellent antifungal activity. Pure silica gel column chromatography was performed on 1.1 g of the purified product. The column size was 2.5 cm × 50 cm and chloroform: methanol (1: 1, v / v) was used as the mobile phase. The mobile phase was flowed at a rate of 1.5 ml per minute and a total of eight tablets were obtained.

Similarly, the antifungal activity was evaluated in the same manner as in <Example 2-2> with respect to the total eight purified products. ) And 8 (GB-9-8, 114 mg) tablets showed the highest activity. Figure 3 shows the results of the antifungal activity test of the 4th, 7th and 8th purification.

When the 4th, 7th and 8th purifications were developed on TLC (silica gel 60 F 254, chloroform; methanol = 1: 1, v / v), the Rf was 0.6, 0.3, 0.28 and showed a single spot, respectively. 4 is a schematic diagram showing the separation process of the active material.

Example 4 Identification of Active Material

Example 4-1 Identification of the Active Substance in Purified Product No. 4 (GB-9-4) -Anagalrosaponin IV

In order to analyze the structure of the isolated active material, 20 mg of purified No. 4 was dissolved in methanol-d4 and the NMR spectrum was measured. Eight methyl groups were identified in the 1 H NMR spectrum (FIG. 5), and one of them was identified as a methyl group (δ 2.1 ppm, s) connected to an acetyl group. In addition, it was expected that the glycoside form was determined from signals around 5-3.0 ppm. 13C NMR spectrum (Fig. 6) was confirmed from the carbonyl carbon of the acetyl group 172.6ppm 107.3, 105.6, 104.8, and the expected that attached to the glycoside per delayed four into four anomeric carbon at 104.3 per literature (Phytotherapy research , 12, s70-s73, 1998) and 13-C NMR chemical shift. -O- [β-D-xylopyranosyl- (1 → 2) -O-β-D-glucopyranosyl- (1 → 4)]- It was confirmed that the glycoside in the form of [O-β-D-glucopyranosyl- (1 → 2)]-O-α-L-arabinopyranosyl. In addition, it was confirmed that 8 quaternary carbons, 24 CH, 14 CH ₂ , and 8 CH ₃ in the DEPT spectrum (FIG. 6) showed that the total carbon number of the compound was 54. Excluding the carbon number per 4 from the total carbon number of the compound, the structure of the aglycone having 32 carbon atoms was expected to be a triterpenoid form composed of 5-rings bound by acetyl groups. In addition, the 77.5ppm signal, CH ₂ , was moved to the low magnetic field, indicating that the epoxy was bound to oxygen in the ring. Therefore, as a result of searching the literature ( Chem . Phram . Bull . 42 (9) 1750-1755, 1994), the structure of the active substance was identified as Anagalarosponin IV of [Formula 1].

LC-MS / MS analysis was performed to determine the molecular weight of the identified material. Anagallosaponin IV had a molecular weight of 1104.57 and was analyzed to be 1128.19 in sodium-bound form by LC-MS / MS analysis. As a result of performing MS / MS for more accurate structure confirmation, it was possible to obtain Anagallosaponin IV-specific cleavage patterns as shown in FIG. 7.

Example 4-2 Identification of the Active Substance in Purified Product No. 7 (GB-9-7) -Anagalrosaponin Ⅷ

In order to analyze the structure of the separated active material, 20 mg of purified No. 7 was dissolved in methanol-d4 and NMR spectrum was measured. Seven methyl groups were identified in the 1 H NMR spectrum (FIG. 8), and one of them was identified as a methyl group (δ 2.1 ppm, s) connected to an acetyl group. In addition, it was expected that the glycoside form was determined from signals around 5-3.0 ppm. 13C NMR spectra (Figure 9) were confirmed at the carbonyl carbon of the acetyl group 172.6ppm 107.4, 104.8, 104.8, and the expected that attached to the glycoside per delayed four into four anomeric carbon at 104.3 per literature (Phytotherapy research , 12, s70-s73, 1998) and 13-C NMR chemical shift. -O- [β-D-xylopyranosyl- (1 → 2) -O-β-D-glucopyranosyl- (1 → 4)]- It was confirmed that the glycoside in the form of [O-β-D-glucopyranosyl- (1 → 2)]-O-α-L-arabinopyranosyl. 13C NMR spectra predicted that the total carbon number of the compound was 54, and the structure of the aglycone with 32 carbon atoms, excluding the carbon number per 4 from the total carbon of the compound, was expected to be in the form of tirterpenoids composed of five rings bound by acetyl groups. It was. Unlike Anagallosaponin IV, 13OH 28.3ppm signal disappeared and 64.6ppm signal appeared, suggesting that OH was bound to methyl group 23. In addition, the signal of 77.5ppm showed that the epoxy form. Therefore, as a result of searching the literature ( Chem . Phram . Bull . 42 (9) 1750-1755, 1994), the structure of the active substance was identified as Anagalarosponin 의 of [Formula 2].

LC-MS / MS analysis was performed to determine the molecular weight of the identified material. Anagalarosponin VIII had a molecular weight of 1120.57 and was analyzed to be 1144.05 in sodium-bound form by LC-MS / MS analysis. As a result of performing MS / MS to confirm the structure more accurately, it was possible to obtain the cleavage pattern inherent in anagalaroponin Ⅷ as shown in FIG. 10.

Example 4-3 Identification of the Active Substances in Purification No. 8 (GB-9-8) -Anagallysin C

In order to analyze the structure of the isolated active material, 20 mg of purified No. 8 was dissolved in methanol-d4 and NMR spectrum was measured. Six methyl groups were identified in the 1 H NMR spectrum (FIG. 11). In addition, it was expected that the glycoside form was determined from signals around 5-3.0 ppm. In addition, unlike the compounds 4 and 7 identified above, no carbonyl carbon of the acetyl group was observed in the 13C NMR spectrum (FIG. 12), and 4 sugars were obtained from 4 anomer carbons per 107.3, 104.7, 104.7, and 104.2. In comparison with the expected glycosides ( Phytotherapy research , 12, s70-s73, 1998) and the 13C NMR chemical shift, -O- [β-D-xylopyranosyl- (1 → 2) -O-β It was confirmed that the glycoside in the form of -D-glucopyranosyl- (1 → 4)]-[O-β-D-glucopyranosyl- (1 → 2)]-O-α-L-arabinopyranosyl. 13 NMR showed that the total carbon number of the compound was 52. When the total carbon number of the compound was excluded, the structure of the aglycone having 30 carbon atoms was expected to be in tirterpenoid form. And 64.5ppm signal was confirmed in the same manner as the single compound 7 and the methyl group 23 was confirmed that the OH is bound form, it can be seen that the epoxy form of the 77.5ppm signal. Therefore, as a result of searching the literature ( Chem . Phram . Bull . 42 (9) 1750-1755, 1994), the structure of the active substance was identified as anagalicin C.

LC-MS / MS analysis was performed to determine the molecular weight of the identified material. Anagallysin C had a molecular weight of 1062.23 and was analyzed to be 1086.13 in sodium-bound form by LC-MS / MS analysis. As a result of performing MS / MS to confirm the structure more accurately, it was possible to obtain the cleavage pattern peculiar to analagasin C as shown in FIG. 13.

Claims (9)

Plant disease control composition by magnaporte grisea comprising magpie swimming extract, fractions of magpie swimming extract, angalalo saponin IV, angalalo saponin Ⅷ, or desgluco galloside B as an active ingredient.
The method of claim 1,
The extract is a plant disease control composition according to Magna Porte Grisce characterized in that obtained by dipping magpie swimming in a mixed solvent of water and ethanol.
The method of claim 1,
The fraction is one of the following (I) and (II) plant composition for controlling plant diseases by Magna Porte Grisse
(I) a fraction of any solvent layer obtained by suspending magnolia swimming in a mixed solvent of water and ethanol, suspended in a mixed solvent of methanol and water, and then fractionating with butanol or hexane; And
(II) Hexane layer or butanol obtained when the extract obtained by dipping magpie swimming in a mixed solvent of water and ethanol is suspended in a mixed solvent of methanol and water, and then sequentially fractionated using hexane, dichloromethane, ethyl acetate and butanol. Fractions of layers.
The method of claim 2,
The mixed solvent of ethanol and water is a plant disease control composition of Magna Porte Grisse, characterized in that 70 to 90% ethanol.
The method of claim 3,
The mixed solvent of ethanol and water is 70 to 90% ethanol, and the mixed solvent of methanol and water is 10 to 40% methanol.
The method of claim 1,
The composition comprises a pesticide acceptable carrier and at least one auxiliary agent selected from the group consisting of wetting agents, dispersants, antifreeze agents, thickeners, preservatives and electrodeposition agents, plant disease control by Magna Porte Grisce Composition.
The method of claim 1,
The composition is a plant disease control agent composition according to Magna Porte Grissa, characterized in that the plant disease caused by Magna Porte Grisse is a plant disease affecting rice, grass, wheat, barley or blood.

A method for controlling a plant disease by Magnaporte grisea comprising the step of contacting the plant composition with the plant disease composition according to any one of claims 1-7.
The method of claim 8,
The plant is a rice, grass, barley, wheat or blood method of controlling plant diseases by Magna Porte Grisse, characterized in that.

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