KR20130109646A - Composition and method for controlling plant disease caused by rhizoctonia cerealis or rhizoctonia solani using an extract of albizzia julibrissin - Google Patents

Abstract

PURPOSE: A composition for preventing plant diseases is provided to show an antifungal activity against Rhizoctonia cerealis and Rhizoctonia solani using an Albizzia julibrissin extract and to prevent plant diseases. CONSTITUTION: A composition for preventing plant diseases caused by Rhizoctonia cerealis and Rhizoctonia solani contains an Albizzia julibrissin extract or a butanol fraction of the extract as an active ingredient. The extract is prepared from Albizzia julibrissin leaves or fruits with C1-C5 alcohols, water, or a mixture thereof. A method for preventing the plant diseases comprises the step of making the composition and a plant come in contact.

Description

Composition and Method for Controlling Plant Disease Caused by Rhizoctonia cerealis or Rhizoctonia solani Using an Extract of Albizzia julibrissin}

The present invention is Albizzia julibrissin ) relates to a plant disease control composition and a method for controlling plant disease by lyxtonia cereals or lyxtonia solani .

In 2009 and 2010, some wheat cultivation areas in Jeollanam-do and North Jeolla Province caused yellowing, leaf spots and reduced yields due to stem death and dobok. The cause was caused by Rhizoctonia cerealis . It was confirmed by a sharp eyespot. Raiztonian cereals kill young seedlings of wheat or cause mozzarella, but more frequently form lesions on the stem base of plants (Lipps and Herr, Phytopathology 72: 1574-1577, 1982; Clarkson et al., Plant Pathology 32: 421- 428, 1983; Cromey et al., New Zealand J. Crop and Hort. Sci. 30: 9-17, 2002) If the onset persists, premature maturation or damage to lodging occurs (Cromey et al., Plant Pathology 58: 268-272, 2005). ). In Korea, Kim et al. (Korean Society of Plant Pathology 7: 52-54, 1991) report the distribution and pathogenicity of the soil in Korea, so it was named as Wheat Leaf Crest Disease. It is also a disease caused by R.cerealis in Korea, besides the sharp eyespot of wheat and barley, sheath eyespot (Kim and Kim, Kor. J. Plant Pathol. 4: 207-217 , 1988), yellow patch of grass (Kim et al., Korean Journal of Grassland 6: 99-112, 1992).

On the other hand, Rhizoctonia solani is a soil infectious pathogen, a multi-purpose pathogen with a wide host in the world, and vegetables such as radish leaf blight, rice leaf blight blight, tomato, eggplant, bell pepper, cabbage and green onion It is known to cause pathogens such as mozzarella disease, rice husk blight, strawberry sprout blight, and horseradish stem rot (Kwan Wan-gyu, 1995. Diagnosis and control of crop Leytonia disease).

Raizantonia solany also invade seeds of crops such as beans, eggplants, peppers, and tomatoes. Once settled in the soil, they can continue to exist. Raiztonia solani are spread from infected areas to healthy areas by rainwater, irrigation, flooding, and other farming equipment.

Most Lysatonia solani have an infectious temperature of 15 to 18 ° C, and some species cause disease well even at temperatures up to 35 ° C. In general, plant diseases caused by Raiztonia solani are more likely to occur in moderately wet soils than in flooded or dry soils. Infection of young plants is also more severe if the plants grow slowly due to inadequate circumstances.

Currently, there is a chemical control method using thiophanate methyl as a method for controlling plant diseases caused by lysatonia fungi, and Stenotrophomonas maltophilia BW as an antagonist against lyc tonia solani . -13 strain (Kim et al., Res. Plant Dis. 11 (2): 152-157, 2005), and trichoderma harzianum (Lee et al., Plant pathology journal 27 (1): 68 -77, 2011), Bacillus amyloliquefaciens KS-4 (Bacillus-amyloliquefaciens KS-4, Accession No .: KACC 91247P) and the like (Korean Patent No. 10-0781472), clove (Eugenia caryophyllata) Biological control methods, such as the use of cloves, have been proposed.

The present invention also provides a method using a silk tree extract as a biological control method.

An object of the present invention is to provide a plant disease control composition by lyxtonia cereals or lyxtonia solanie using the extract of Albizia julibrissin.

It is another object of the present invention to provide a method for controlling plant diseases by Raiztonia cereals or Raiztonia solani using the extract of Albizia julibrissin.

It is another object of the present invention and specific objects will be presented below.

In the present invention, 80% ethanol extracts of the leaves and berries of the larvae and the sequential fractions of the extracts of the butanol layer are the causative agents of the causative organisms such as lysatonia cereals and radish leaf blight, which are the cause of wheat leaf stalk disease. It was completed by confirming that Tonia Solani has antifungal activity.

In consideration of the above, the control agent composition of the present invention is characterized in that it comprises a fraction of the butanol layer of the extract of the Albizia julibrissin or the Albizia julibrissin as an active ingredient.

In addition, in the present specification, "extracted tree extract", regardless of the extraction method, the extract of the tree branches, leaves, berries, roots or mixtures thereof, the lower alcohol having 1 to 5 carbon atoms, such as methanol, ethanol, butanol, water or these Means an extract obtained by extraction with a mixed solvent of. Regardless of the extraction method, it is to be understood that the extraction method may be applied to any method such as cooling, refluxing, heating, ultrasonic radiation, and the like, as long as the extraction object is extracted through immersion in the extraction solvent. Nevertheless, the "Prune extract" means preferably an extract obtained by extracting with water, ethanol or a mixed solvent thereof, more preferably a mixed solvent of water and ethanol, in particular 70-90% aqueous ethanol solution (% Means v / v). The extract may be filtered, concentrated and the phase may be liquid or powder.

In addition, in the present specification, the "fraction of the butanol layer of the silk tree extract" refers to the solid silk tree extract obtained from the silk tree extract, preferably a mixed solvent of water and ethanol, in particular 70-90% ethanol aqueous solution. Suspension of a butanol layer obtained by suspending in a mixed solvent of water, in particular 10-40% methanol, and then sequentially dividing with hexane, dichloromethane, ethyl acetate and butanol. It is meant to include a fraction of the butanol layer obtained by suspending in a mixed solvent, in particular, 10-40% methanol, adding butanol, leaving the mixture to stand, and then separating the butanol layer. By "sequentially fractionating" here is meant that fractions of a mixed solvent of water and methanol are continued to be used after fractionation to fractionate into solvents in the order listed above.

Herein, the meaning of the above-mentioned "active ingredient" means an ingredient that alone exhibits the desired activity or can exhibit activity together with a carrier which is itself inactive.

Also in this specification, "control" means the prevention, treatment or alleviation of symptoms of plant diseases caused by Laytonia serials or Laitonia solani.

In addition, in the present specification, "plant diseases caused by Raiztonia cereals or Raiztonia solani" is a case of plant diseases caused by Raiztonia cereals, as shown above, leaf blight of wheat and barley, wheat seedling disease , Rice leaf blight, grass pressed blight, plant disease caused by Raizonia solani, radish leaf blight, rice blight blight, tomato, eggplant, bell pepper, cabbage, green onion and other vegetables It includes stem rot diseases such as rice husk blight, strawberry sprout blight and wasabi.

The control agent composition of the present invention may be included in any amount within the range of 1 to 99% by weight of the active ingredients, depending on its formulation, subjects, parts, etc., based on the total weight of the control agent composition of the present invention, It will typically be included in the range of 1 to 30% by weight, usually in the range of 10 to 20% by weight in liquids, and typically in the range of 30 to 40% by weight in powders.

The control composition of the present invention may be prepared as a formulation of the active ingredients, or may be mixed with an agriculturally acceptable carrier to be formulated into an emulsion, solution, suspension, hydrating agent, flow agent, powder, granule, dispersant, and the like.

In the above, the "agrochemically acceptable carrier" is combined with the active ingredient of the control agent composition of the present invention to promote the spreading, storage and / or transport of the active ingredient without inhibiting the activity of the active ingredient and acceptable to plants It refers to any substance that does not exhibit the above toxicity.

Carriers that can be used are soil natural minerals (e.g. kaolin, clay, talc, chalk), soil synthetic minerals (e.g. highly dispersed silica, silicate), carbon based materials (charcoal, bitumen, etc.), sulfur, natural or synthetic Resins, fertilizers, cellulose based materials (sawdust and corncobs), water, aromatic solvents (e.g. Solvesso products, xylene), paraffins (e.g. mineral oil fractions), alcohols (e.g. methanol, butanol, pentanol, Benzyl alcohol), ketones (e.g. cyclohexanone, methyl hydroxybutyl ketone, diacetone alcohol, mesityl oxide, isophorone), lactones (e.g. gamma-butyrolactone), pyrrolidone (pyrrolidone) , N-methylpyrrolidone, N-ethylpyrrolidone, n-octylpyrrolidone), acetates (glycol diacetate), glycols, dimethyl fatty acid amides, fatty acids, fatty acid esters, and mixtures thereof.

The control composition of the present invention may also include one or more adjuvants such as wetting agents, dispersants, antifreezes, thickeners, preservatives, electrodeposition agents.

Such adjuvants may be selected and used appropriately from those known in the art, for example, alkylnaphthalene sulfonate (particularly diisopropylnaphthalenesulfonate or diisobutylnaphthalenesulfonate) may be used as the wetting agent, and nonionic as a dispersant. Dispersants (ethylene oxide / propylene oxide block polymers, alkylphenol polyglycol ethers, tristyrylphenol polyglycols, etc.), anionic dispersants (ligninsulfonic acid, naphthalenesulfonic acid, phenolsulfonic acid, dibutylnaphthalenesulfonic acid, alkylarylsulfonates, alkyl sulfides) Pate, alkylsulfonate, etc.) or mixtures thereof, methanol, ethanol, isopropanol, butanol, glycol, glycerin, diethylene glycol and the like can be used, and as a thickener, cellulose derivatives, polyacrylic acid derivatives, xanthan Modified clay As the preservative, dichlorophene, isothiazolene, benzyl alcohol, etc. can be used, and the electrodeposition agent is polyvinyl alcohol, polyvinylpyrrolidone, polyacrylate, polymethacrylate, polybutene, polyisobutylene , Polystyrene, polyethyleneamine, polyethyleneamide, polyethyleneimine, polyether and the like can be used.

The control composition of the present invention may further contain other known components such as herbicide components, insecticidal components, bactericidal components, etc., depending on the purpose of use, the environment of the arable land, and the like.

In another aspect, the present invention relates to a method for controlling plant diseases by Magna Porte Grisea using the control composition of the present invention as described above.

Plant disease control method by Magna Porte Grisse of the present invention is to contact the plant composition of the present invention to wheat, barley, rice, grass, radish, tomato, eggplant, bell pepper, cabbage, vegetables, strawberries, horseradish Characterized in that it comprises a step.

The contacting step will usually be achieved by spraying on affected rice, grass, barley, wheat, etc., but by spraying or incorporating into soil, which is a habitat environment of rice, grass, barley, wheat or the like, or spraying on seeds or seedlings thereof. Or by spraying or mixing the growth medium or soil in its habitat.

Alternatively, the step of contacting may be made by mixing with a fungicide, insecticide, herbicide, fertilizer and the like.

How much to use the active ingredient of the control agent of the present invention in plants such as rice, grass, barley, wheat, etc., the growth degree of rice, grass, barley, wheat and the like, the degree of disease, soil conditions, rainfall, use It will be determined by considering the kind of active ingredient and the like comprehensively. Usually, it is used at 10 to 500 g, preferably 30 to 250 g per hectare, and when spraying and mixing the soil, it is used at 10 to 1000 g, preferably 100 to 500 g per hectare.

As described above, according to the present invention, the control composition of plant diseases caused by Cerealis or Lysatonia solani and the Cerealis using the composition or the extract of the silk tree extract and butanol layer fractionated from the extract It is possible to provide a method for controlling plant diseases by Laitonton solani.

Figure 1 is a schematic diagram of the production process of the extract of the silk tree leaf and fractions.
Figure 2 is a schematic diagram of the manufacturing process of the extract and the silk tree fruit.
Figure 3 is a photograph showing the antifungal activity of the leaf extract and the fraction of Lycitonia cereals.
Figure 4 is a photograph showing the antifungal activity against the lysatonia cereals of the extract and fractions of the Albizia julibrissin.
Figure 5 is a photograph showing the antifungal activity against the lysatonia solani of the extract and the silk leaf.
Figure 6 is a photograph showing the antifungal activity of the Lycitonia solanie of the extract and fractions of the Albizia julibrissin.

Hereinafter, the present invention will be described with reference to Examples and Experimental Examples. However, the scope of the present invention is not limited to these examples and experimental examples.

< Example > With extracts of the leaves and berries Fraction  Produce

<Example 1> Preparation of Albizia tree leaf extracts and fractions

3 kg of silk leaf leaf powder powder was added to 60 L of 80% ethanol, extracted by stirring for 24 hours, filtered through a filter paper (Advantec NO.2), the filtrate was concentrated under reduced pressure and lyophilized to obtain a powdery extract.

10 g of the obtained ethanol extract was suspended and dissolved in 100% by weight of 20% methanol, followed by hexane (n-hexane), dichloromethane (CH ₂ Cl ₂ ), ethyl acetate (EtOAc) and butanol (BuOH). Fractions yielded a fraction of 0.9123 g in the hexane layer, 0.3472 g in the dichloromethane layer, 0.7619 g in the ethyl acetate layer, 3.5719 g in the butanol layer and 4.3976 g in the remaining layer (20% methanol solvent layer).

A schematic diagram of the fraction preparation process from the 80% ethanol extract and 80% ethanol extract is shown in FIG.

<Example 2> Preparation of silk tree fruit extract and fractions

5.648kg of the Albizia julibrissin powder powder was added to 100L of 80% ethanol, followed by extraction with stirring for 24 hours. The filtrate was concentrated under reduced pressure and freeze-dried to obtain a powdery extract.

10 g of the obtained ethanol extract was suspended and dissolved in 100% of 20% methanol, and then sequentially with hexane (n-hexane), dichloromethane (CH ₂ Cl ₂ ), ethyl acetate (EtOAc) and butanol (BuOH). Fractions yielded a fraction of 0.6273 g in the hexane layer, 0.2012 g in the dichloromethane layer, 0.3025 g in the ethyl acetate layer, 3.3146 g in the butanol layer and 5.204 g in the remaining layer (20% methanol solvent layer).

A schematic of the process for preparing fractions from the 80% ethanol extract and 80% ethanol extract is shown in FIG. 2.

< Experimental Example > With extracts of the leaves and berries Fraction  Raiztonia Cereals  And Raiztonia Solanie  About Antifungal  Activity evaluation

In order to evaluate the antifungal activity of extracts and fractions of the leaves and berries of Raspberry on Lysatonia cereals and Lysatonia solani, After 5 days of incubation on potato agar medium (PDA, potato dextrose agar), 6 mm diameter mycelia were taken from the edges of the flora, and 50 µl each of extract and 40 µl of fractions of 50 ml / mL paper disk were 8 mm in diameter. After dropping in, inoculated in potato agar medium and cultured for 27 to 5 days to measure the production of the flora.

The antifungal activity of the extract and the extract of the Albizia julibrissin on Lysatonia cereals are shown in FIG. The antifungal activity of the extract and the fraction of the Albizia julibrissin against Solani are shown in FIG.

3 to 6, 1 is control (EtOH 50% itself), 2 is residue layer (H ₂ O), 3 is 80% ethanol extract (Crude extract), 4 is butanol layer fraction (BuoH), 5 is control (EtOH 100% itself), 6 is a fraction of the hexane layer (Hex), 7 is a fraction of the ethyl acetate layer (CH ₂ Cl), 8 is a fraction of the ethyl acetate layer (EtoAc).

Referring to Figures 3 to 6, the high anti-gom palm activity in the order of fractions of the butanol layer and 80% ethanol extract for both lyxonia serials and lyxonia solani for all the larva leaves / fruit extracts / fractions. And the remaining fractions showed no activity.

Claims (8)

Contains the fraction of the butanol layer of the silk extract or the silk extract as an active ingredient
Plant disease control composition by lyxtonia cereals or lyxtonia solani using a silk extract.
The method of claim 1,
The extract of the Albizia julibrissin is an extract obtained by extracting a nymph leaf or a fruit with a lower alcohol having 1 to 5 carbon atoms, water or a mixed solvent thereof.
Plant disease control composition by lyxtonia cereals or lyxtonia solani using a silk extract.
The method of claim 1,
The fraction of the butanol layer of the extract of the silk tree is characterized in that the fraction of (I) below or the fraction of (II) below
Plant disease control composition by lyxtonia cereals or lyxtonia solani using a silk extract.
(I) A fraction of the butanol layer obtained when the solid silkworm extract obtained as a mixed solvent of water and ethanol is suspended in a mixed solvent of methanol and water, and then sequentially partitioned into hexane, dichloromethane, ethyl acetate and butanol.
(II) A fraction of the butanol layer obtained by suspending the solid silk tree extract obtained from the mixed solvent of water and ethanol in a mixed solvent of methanol and water, adding butanol and standing still, and then separating the butanol layer.
The method according to claim 2 or 3,
The mixed solvent of ethanol and water is characterized in that 70 to 90% ethanol
Plant disease control composition by lyxtonia cereals or lyxtonia solani using a silk extract.
The method of claim 3,
The mixed solvent of ethanol and water is 70 to 90% ethanol, the mixed solvent of methanol and water is characterized in that 10 to 40% methanol
Plant disease control composition by lyxtonia cereals or lyxtonia solani using a silk extract.
The method of claim 1,
The composition further comprises an agriculturally acceptable carrier and one or more adjuvants selected from the group consisting of wetting agents, dispersants, antifreezes, thickeners, preservatives and electrodeposition agents.
Plant disease control composition by lyxtonia cereals or lyxtonia solani using a silk extract.
A method comprising contacting a plant with a plant disease composition according to any one of claims 1 to 6, wherein
A method for controlling plant diseases by Laitontonia cerealless or Laitontonia solanie.
The method of claim 7, wherein
The plant is wheat, barley, rice, grass, radish, tomato, eggplant, bell pepper, cabbage, vegetables, berries or horseradish
A method for controlling plant diseases by Laitontonia cerealless or Laitontonia solanie.

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