KR101158203B1 - Composition comprising the extract of Opuntia humifusa for preventing or treating uterine cervical cancer - Google Patents

Abstract

The present invention relates to a pharmaceutical composition and a health functional food composition for the prevention or treatment of cervical cancer, which contains an extract of the millennial cactus organic solvent as an active ingredient. The millennial cactus organic solvent extract according to the present invention has relatively low side effects and toxic expression rate, is bio-friendly, and has an effect of inhibiting the growth of cervical cancer cells, delaying cell cycle and apoptosis, and thus useful for preventing or treating cervical cancer. Can be used.

Description

Composition for the extract of Opuntia humifusa for preventing or treating uterine cervical cancer}

The present invention relates to a composition for the prevention or treatment of cervical cancer containing Opuntia humifusa organic solvent extract as an active ingredient. More specifically, the pharmaceutical composition for the prevention or treatment of cervical cancer containing the millennial cactus organic solvent extract, which can prevent or treat cervical cancer as an active ingredient, by inhibiting the growth of cervical cancer cells, delaying cell cycle and inducing apoptosis. A composition and a nutraceutical composition.

Advances in technology have made it possible to treat many diseases. Nevertheless, chronic degenerative diseases caused by environmental factors such as eating habits are increasing, and cancer is a representative disease and is a great threat to human welfare in modern society. In the case of cervical cancer, in particular, the prevalence decreases due to early diagnosis, but it is still the cause of cancer mortality in women after breast cancer.

Currently, the development of treatment for cancer patients has been continuously studied, but most of the anti-cancer drugs widely used in everyday life are synthetic materials, and the side effects are very serious, and the problem is frequently raised. Recently, in order to develop effective anti-cancer drugs with few side effects, a lot of biologically active substances are searched from natural plant sources. Therefore, it is necessary to develop anti-cancer agents derived from natural products with low toxicity and effective to prevent cancer after developing cancer.

On the other hand, palm cactus is one of the 4,000 species of cactus known all over the world. Mostly tropical and subtropical, Opuntia ficus-indica , an introduced species in Korea, is cultivated and produced mainly in the warm province of Jeju Island. Many studies have already been made at home and abroad and commercialized in the form of health food.

In contrast, Opuntia humifusa is a kind of palm cactus, but it is a perennial plant that grows in the temperate zone with four distinct seasons and can be overwintered. It is a native cactus of Korea, and it can be produced by open field cultivation throughout the Korean peninsula. It is mainly produced in the region (Han Seung-ho, Kim Gil-su, Kim Bok-hyun, etc., development of safe production technology for natural cactus and development of treatment for contact dermatitis, 2008).

Millennial cactus is not only less known than early centennial, but little information is revealed through research. Millennial cactus is an early research stage, and it is a native cactus, which has been studied mainly in Korea, and studies on the effects of osteoporosis prevention and breast cancer cell growth inhibition (Korean J. Food & Nutr. Vol. 2009) and anticancer activity of cervical cancer cell line using fermentation is known. In particular, Korean Patent No. 10-0698533 discloses a method for preparing a composition for the prevention and treatment of antimicrobial infections and anticancer activity using fermentation broth of wintering cactus, but water, methanol and ethanol extracts do not have anticancer activity. It is described that it should go through the step of adding fermentation. Therefore, in the case of the patent, the process has to be complicated by adding a yeast or a lactic acid bacterium.

Natural second cactus compared to baeknyeoncho phenol (phenol) and flavonoids (flavonoid) component content is higher functionality seen by periods, such as on the human health identified as bar is expected to excel and more varied, the present inventors have cheonnyeoncho Cactus (Opuntia humifusa As a result of continuous studies on the prevention or treatment of cervical cancer by the hexane and ethyl acetate extract of), it was confirmed that the inhibition of cell proliferation by MTT assay in HeLa cells, cervical cancer cells, and flow cytometry. Through the confirmation of the delay of cell division and induction of apoptosis, the present invention was completed.

Therefore, it is an object of the present invention to provide a pharmaceutical composition for the prevention or treatment of cervical cancer, which contains the cheonnyeoncho cactus organic solvent extract as an active ingredient.

Another object of the present invention is to provide a health functional food composition for the prevention or improvement of cervical cancer, which contains the cheonnyeoncho cactus organic solvent extract as an active ingredient.

Other objects and advantages of the present invention will become more apparent from the following detailed description of the invention, claims and drawings.

In order to achieve the above object, the present invention provides a pharmaceutical composition for the prevention or treatment of cervical cancer, containing the cheonnyeoncho cactus organic solvent extract as an active ingredient.

In addition, the present invention provides a health functional food composition for the prevention or improvement of cervical cancer, containing the cheonnyeoncho cactus organic solvent extract as an active ingredient.

The composition is characterized in that the induction of cell cycle delay and apoptosis of cervical cancer cells.

In addition, the organic solvent is characterized in that the hexane or ethyl acetate.

Hereinafter, the present invention will be described more specifically.

The present invention provides a composition for the prevention or treatment of cervical cancer, containing the cheonnyeoncho cactus organic solvent extract as an active ingredient.

In the present invention, the composition was treated to cervical cancer cells (HeLa) to evaluate the effect of the cheonnyeoncho cactus hexane and ethyl acetate extract on cervical cancer cell growth inhibition and cell cycle. The aim of this study was to determine the possibility of preventing or improving cervical cancer of Chun-Yang Cactus Extract.

As a result, the composition containing the millennial cactus organic solvent extract according to the present invention has been shown to be effective in inhibiting cervical cancer cell growth and cell cycle progression. More specifically, the effect of inhibiting cancer cell proliferation of HeLa cells, which are cervical cancer cells, of the millennial cactus organic solvent extract according to the present invention is as follows. When hexane extracts were treated at concentrations of 100, 500, and 1000 ㎍ / ml, all proliferation of HeLa cells was significantly inhibited. When the concentration of extract was added at 500 ㎍ / ml, the inhibitory effect was higher than 50%. (FIG. 1). And cancer cell growth inhibitory effect of ethyl acetate extract on HeLa cells showed more than 40% proliferation inhibitory effect in OHF-E and OHR-E (Fig. 2). In addition, when hexane or ethyl acetate extract was treated with 100 ㎍ / ml and incubated for 24, 72, 120 hours it was shown to inhibit the cervical cancer cells in a time-dependent manner (Fig. 3 and 4).

On the other hand, tissue homeostasis is regulated by the balance between cell proliferation and apoptosis, and cancer is caused when cell proliferation, cell cycle progression, and apoptosis have an unbalanced balance. Therefore, inhibiting cell proliferation of cancer cells, delaying cell cycle progression and inducing apoptosis are effective methods of inhibiting cancer. In the present invention, by selecting the extract of the millennial cactus effective in the above cytotoxicity experiments and investigated the effect on the cell cycle progression of each cancer cell. After incubating for 24 hours by adding 100 ㎍ / ml of the extract to the cell culture, the cell cycle was analyzed by flow cytometry by staining cellular DNA with propidium iodide.

As a result, as shown in Figure 5, in the HeLa cells treated with millennial cactus OHF-E, OHS-H, OHSt-H significantly increased G1 cells compared to the control group (P <.05). On the other hand, cells of S and G2 / M phases were significantly decreased by P. C. extract (P <.05), indicating that C. C. extract induced G1 arrest of HeLa cells. Treatment with OHF-E and OHR-E showed an increase in sub-G1 cells. This can be interpreted as an increase in cells in which apoptosis has occurred. HeLa cells were treated with OHF-E, OHSt-E and OHS-H at 100 ㎍ / ml for 24 hours, and the number of non-apoptotic cell deaths in the treated extracts was significantly higher than that of the control. It was found to increase (P <.05), especially the ethyl acetate extract of the stem showed the highest apoptosis effect (Fig. 6).

Therefore, the composition containing the cheonnyeoncho cactus organic solvent extract according to the present invention as an active ingredient can be used as a pharmaceutical composition for the prevention or treatment of cervical cancer based on natural extracts.

In the composition of the present invention, the millennial cactus extract may be extracted using water, an organic solvent or a mixed solvent thereof, which is a well-known extractant for extracting natural products, but preferably hexane or ethyl of cactus millet cactus. As the acetate (ethyl acetate) extract, the extract was shown to have an excellent anticancer effect. Hexane extraction is widely applicable to trace components and is mainly used for oil extraction. The concentration of hexane and ethyl acetate is not particularly limited, but when the hexane or ethyl acetate of 80% or more can increase the extraction efficiency. The hexane extract may be further fractionated using an organic solvent having a different polarity, and may be further purified using various chromatography.

In the composition of the present invention, the cheonnyeoncho cactus extract is not particularly limited in the extraction site, and is an organic solvent extract of fruit (seeds left after separating the seeds from the fruit), seeds, stems and roots, not the fermentation broth itself. It is extracted using. However, hexane extracts of cheonnyeoncho seeds, ethyl acetate extracts of fruits or stems are preferred because they have a high anticancer effect of delaying cell cycle and inducing apoptosis.

In the present invention, the extract should include any of the extracts, fractions and purified products obtained in each step of extraction, fractionation and purification, any dilution or concentrate thereof, or dried products thereof.

In the composition of the present invention, preferably the cheonnyeoncho cactus extract based on the total weight of the composition may comprise 0.0001 to 90% by weight. In the compositions of the present invention, they may be formulated in the form of powders, granules, tablets, capsules, injections, creams, gels, patches, sprays or ointments by conventional methods known in the art.

In addition, the pharmaceutical composition containing the millennial cactus organic solvent extract of the present invention as an active ingredient may further include appropriate carriers, excipients and diluents commonly used in the manufacture of pharmaceutical compositions. Carriers, excipients and diluents that may be included in the composition containing the millennial cactus extract as an active ingredient include lactose, dextrose, sucrose, oligosaccharide, sorbitol, mannitol, xylitol, erythritol, maltitol, starch, acacia rubber, alginate , Gelatin, calcium phosphate, calcium silicate, cellulose, methyl cellulose, microcrystalline cellulose, polyvinylpyrrolidone, water, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate and mineral oil.

When formulated, diluents or excipients such as fillers, extenders, binders, wetting agents, disintegrating agents, and surfactants are usually used. Solid preparations for oral administration include tablets, pills, powders, granules, capsules, and the like, and the solid preparations may include at least one excipient such as starch, calcium carbonate, sucrose, or the like in the extract. Or lactose, gelatin, or the like is mixed. In addition to simple excipients, lubricants such as magnesium styrate talc are also used. Oral liquid preparations include suspending agents, liquid solutions, emulsions, and syrups, and may include various excipients, such as wetting agents, sweeteners, fragrances, and preservatives, in addition to commonly used simple diluents such as water and liquid paraffin. . Formulations for parenteral administration include sterile aqueous solutions, non-aqueous solvents, suspensions, emulsions, lyophilized preparations, suppositories. As the non-aqueous solvent and suspending agent, propylene glycol, polyethylene glycol, vegetable oil such as olive oil, injectable ester such as ethyl oleate and the like can be used. Examples of the suppository base include witepsol, macrogol, tween 61, cacao butter, laurin, glycerogelatin and the like.

Preferred dosages of the extracts of the present invention vary depending on the condition and weight of the patient, the extent of the disease, the form of the drug, the route of administration and the duration, and may be appropriately selected by those skilled in the art. For the desired effect, the extract of the present invention is preferably administered at 0.0001 to 1000 mg / kg on a daily basis, 0.01 to l50 mg / kg to be more effective. The administration may be carried out once a day or divided into several times. The dose is not intended to limit the scope of the invention in any way.

Extracts of the invention can be administered by a variety of routes. All modes of administration may be expected, for example, by oral, rectal or intravenous, intramuscular, subcutaneous, intra-uterine or intracerebroventricular injections.

According to another aspect of the present invention, the present invention is to provide a health functional food composition for the prevention or improvement of cervical cancer containing an extract of millennial cactus organic solvent as an active ingredient.

The composition is characterized by inducing growth inhibition of cervical cancer cells, cell cycle delay and apoptosis. Therefore, the food composition containing millennial cactus extract according to the present invention can be used as a health functional food composition for the prevention or improvement of cervical cancer.

In the present specification, the term "food" means a natural product or processed product containing one or more nutrients, and preferably means a state in which it can be directly eaten through some processing process. Includes food, food additives, nutraceuticals and beverages. In the present specification, the health functional food means a food manufactured and processed using raw materials or ingredients having functional properties useful for the human body according to Act No. 6767 of the Health Functional Food Act, and the term "functionality" refers to the structure of the human body and It means ingestion for the purpose of obtaining useful effects on health use such as nutrient control or physiological action with respect to function.

In the food composition of the present invention, preferably the extract may be included in 0.0001 to 90% by weight relative to the total weight of the composition. The food of the present invention may further comprise a food acceptable food supplement, and may be formulated in the form of pills, powders, granules, acupuncture, tablets, capsules or beverages.

For example, the health functional food in the form of tablets may be prepared as it is or by adding an excipient, a binder, a disintegrating agent or other additives to make a granular form in a suitable manner, and then compressing by adding a lubricant or the like or preparing a health in a tablet form. It is made by directly compressing the functional food as it is or by mixing it evenly with an excipient, binder, disintegrant or other suitable additives, or mixing the health functional food as it is or evenly adding the appropriate additive to the prepared granules, The excipient, binder or other suitable additives are added to the functional food, and the powder mixed evenly is wetted with a solvent, the wet powder is molded into a mold at low pressure, and then dried and prepared by a suitable method. In addition, the nutraceutical can be added to the health functional food in the form of tablets, if necessary, can be avoided with a suitable epidermis.

Among the health functional foods in the form of capsules, the hard capsules are usually prepared by mixing the capsules evenly with the health functional foods or the excipients suitable for the health functional foods, granulated by a suitable method or granulated with a suitable epidermal agent as it is or Soft capsules are prepared by filling them, and soft capsules are usually filled with capsules containing glycerin or sorbitol in a capsule form containing gelatin or appropriate excipients suitable for health functional foods or health functional foods. It is molded and prepared, and a coloring agent preservative etc. can be added to the said capsule base as needed.

The health functional food in the form of a cyclic form is usually prepared by mixing excipients, binders, disintegrants and the like evenly in the health functional foods and forming them into spherical forms in a suitable manner. Or they may be greeted with a suitable substance.

In addition, the composition of the present invention can be used as a food supplement of health supplements for the prevention of related diseases. Foods to which the extract of the present invention may be added include various foods, for example, beverages, gums, teas, vitamin complexes, dietary supplements, and the like, which are pills, powders, granules, tablets, tablets, capsules or beverages. Available in form.

At this time, the amount of the extract in the food or beverage, in general, can be added to 0.0001 to 90% by weight of the total food weight of the food composition of the present invention, 0.0001 to 20% by weight based on 100ml for the health beverage composition Can be added.

Food additives other than the extracts defined in the present invention include food additives customary in the art, such as flavors, flavors, colorants, fillers, stabilizers and the like, and are exemplified below.

In the case of the beverage composition, there are no particular limitations on the liquid components contained in the indicated ratios other than the extract as essential components, and may contain various flavors or natural carbohydrates, etc. as additional components, as in general beverages. Examples of the natural carbohydrates include, for example, monosaccharides such as glucose and fructose, for example, disaccharides such as maltose and sucrose, for example, conventional sugars such as polysaccharides such as dextrin and cyclodextrin, and xylitol. Sugar alcohols such as sorbitol and erythritol. As flavoring agents other than those mentioned above, natural flavoring agents (tauumatin, stevia extract (e.g., Rebaudioside A, glycyrrhizin, etc.) and synthetic flavoring agents (saccharin, aspartame, etc.) can be advantageously used. The proportion of natural carbohydrates is generally about 1 to 20 g, preferably about 5 to 12 g per 100 ml of the composition of the present invention.

In addition to the above, the composition of the present invention includes various nutrients, vitamins, minerals (electrolytes), flavors such as synthetic flavors and natural flavors, coloring and neutralizing agents (such as cheese and chocolate), pectic acid and salts thereof, alginic acid and its Salts, organic acids, protective colloidal thickeners, pH adjusters, stabilizers, preservatives, glycerin, alcohols, carbonation agents used in carbonated drinks, and the like. In addition, the compositions of the present invention may contain flesh for the production of natural fruit juices and fruit juice drinks and vegetable drinks. These components can be used independently or in combination. The proportion of such additives is not so critical but is generally selected in the range of 0.001 to about 20 parts by weight per 100 parts by weight of the composition of the present invention.

As described above, the millennial cactus organic solvent extract according to the present invention has an effect of inhibiting the growth of cervical cancer cells, delaying cell cycle and apoptosis, and thus can be effectively used for the prevention or treatment or improvement of cervical cancer. In addition, the cheonnyeoncho cactus extract according to the present invention as a natural extract can relatively low side effects and toxic expression rate and can promote the effect of preventing, treating or improving cervical cancer biocompatible.

Furthermore, the present invention is expected to increase the value of millennial cactus extract and to increase the income of workers in related industries by expanding the application range of millennial cactus extract from simple foods to preventive or therapeutic drugs.

1 is a view showing the effect of inhibiting the growth of cervical cancer cells of cheonnyeoncho cactus hexane extract according to the present invention. Mean ± standard error (n = 3), different superscripts indicate statistically significant difference (p <0.05).
Figure 2 is a diagram showing the effect of inhibiting the growth of cervical cancer cells of cheonnyeoncho cactus ethyl acetate extract according to the present invention. Mean ± standard error (n = 3), different superscripts indicate statistically significant difference (p <0.05).
Figure 3 is a view showing the growth inhibitory effect of cervical cancer cells during prolonged treatment of cheonnyeoncho cactus hexane extract according to the present invention. Mean ± standard error (n = 3), different superscripts indicate statistically significant difference (p <0.05).
Figure 4 is a view showing the growth inhibitory effect of cervical cancer cells during prolonged treatment of the millennial cactus ethyl acetate extract according to the present invention. Mean ± standard error (n = 3), different superscripts indicate statistically significant difference (p <0.05).
Figure 5 is a view showing the cell cycle delay effect of cervical cancer cells during the millennial cactus extract treatment according to the present invention.
Figure 6 is a view showing the apoptosis effect of cervical cancer cells during the treatment of millennial cactus extract according to the present invention.

Hereinafter, the present invention will be described in more detail with reference to the accompanying drawings. It is to be understood by those skilled in the art that these embodiments are only for describing the present invention in more detail and that the scope of the present invention is not limited by these embodiments in accordance with the gist of the present invention .

Example 1. Preparation of Millennial Cactus Extract

1-1. Manufacture of Millennial Cactus Fruit Extract

After lyophilization and pulverization of 1.36 kg of the fruit (pulp from which seeds were isolated) of Opuntia humifusa , 8 L of methanol was added and extracted at room temperature three times at room temperature for 48 to 72 hours, and then filter paper (Whatman No. 2, Maidstone, England) and concentrated with a rotary vacuum evaporator (R-210 Rotavapor, BUCHI Co.) to obtain 230g Cactus fruit extract was used as a sample of the following experimental example (hereinafter referred to as "OHF-M" Business card).

230 g of the millennial cactus fruit extract (OHF-M) was sequentially fractionated with 2 L of hexane, 2 L of ethyl acetate, and 2 L of water, and then concentrated and freeze-dried to obtain 25 g of hexane-soluble fraction (OHF-H) of the millennial cactus. 4 g of (OHF-E) and 201 g of water-soluble fraction (OHF-W) were each obtained and used as a sample for the following experimental example.

1-2. Manufacture of Millennial Cactus Seed Extract

Except for using 1.53 kg of the seed of Opuntia humifusa (Ountia humifusa ), the same process as in Example 1-1 was carried out to obtain 80 g of millennial cactus seed extract was used as a sample of the following experimental example (hereinafter, "OHS -M ”).

80 g of the Millennial Cactus Seed Extract (OHS-M) was sequentially fractionated with 2L of hexane, 2L of ethyl acetate and 2L of water, and then concentrated and freeze-dried to obtain 21 g of hexane-soluble fraction (OHS-H) of the Millennial Cactus Seed (OHS-H) 3.1 g of (OHS-E) and 55.9 g of water-soluble fraction (OHS-W) were each obtained and used as samples in the following experimental example.

1-3. Manufacture of Millennial Cactus Stem Extract

Except for using 800 g of the stem of Opuntia humifusa ( Ountia humifusa ), the same process as in Example 1-1 was obtained to obtain 80 g of the cheonnyeon cactus stem extract was used as a sample of the following experimental example (hereinafter, "OHSt- Named M ”).

80 g of the cheonnyeon cactus stem extract (OHSt-M) was sequentially fractionated with 2L of hexane, 2L of ethyl acetate, and 2L of water, and then concentrated and freeze-dried to obtain 20 g of hexane-soluble fraction (OHSt-H) of cheonnyeon cactus stem, ethyl acetate-soluble fraction 1.7 g of (OHSt-E) and 58.3 g of water-soluble fraction (OHSt-W) were each obtained and used as samples in the following experimental example.

1-4. Manufacture of Millennial Cactus Root Extract

Except for using 16 g of the root of Opuntia humifusa ( Ountia humifusa ), the same process as in Example 1-1 was carried out to obtain 2 g of millennial cactus root extract was used as a sample of the following experimental example (hereinafter, "OHR- Named M ”).

2 g of cheonnyeon's cactus root extract (OHR-M) was sequentially fractionated with 2L of hexane, 2L of ethyl acetate and 2L of water, and then concentrated and freeze-dried to hexane soluble fraction (OHR-H) of 0.35g, ethyl acetate soluble. 0.24 g of fraction (OHR-E) and 1.41 g of water-soluble fraction (OHR-W) were each obtained and used as samples in the following experimental example. The yield of the extract for each part of the millennial cactus is shown in Table 1 below.

menstruum Millennial Cactus ( Opuntia humifusa ) Fruit(%) strain(%) stem(%) Root(%) Methanol 16.91 5.23 10 12.50 Hexane 1.83 1.37 2.50 2.18 Ethyl acetate 0.29 0.20 0.21 1.50 water 14.78 3.65 7.29 8.81

Example 2. Cell Culture

Cancer cell line used in the present invention, HeLa (human cervix adenocarcinoma), which is a cervical cancer cell, was purchased from Korean Cell Line Bank (KCLB). HeLa cell lines in Dulbecco's modified Eagle's medium (DMEM, Caisson, USA) with 10% fetal bovine serum (Gemcell, Gemini, USA) and 1% L-glutamine (Caisson, USA) and 1% penicillin and streptomycin (Caisson). , USA) was used to culture in a 37 ° C 5% CO ₂ incubator.

Example 3. Measurement of cervical cancer cell proliferation inhibitory effect (Cytotoxicity)

Inhibition of cancer cell proliferation of millennial cactus extract was performed using 3- (4,5-dimethylthiazol-2-yl) -2,3 diphenyltetrazolium bromide (MTT) assay (Roche, Germany). Cell lines were dispensed in 96-well plates at a density of 1 x 10 ⁴ cells / well and incubated in a 5% CO ₂ incubator at 37 ° C for 24 hours, followed by extracting 0, 100, 500, 1000 ㎍ / ml It was added at the concentration and incubated for 24 hours. Add 10 μL of MTT labeling reagent (Roche, Germany) to each well, incubate for 4 hours (37 ^o C, 5% CO ₂ ), and add 100 μL of solubilization solution (Roche, Germany) to the incubator. (incubator) was kept for 10 hours, and absorbance was measured at 550 nm with an ELISA reader (PowerWaveXS, Bio-tek, Winooski, USA). The results are shown in FIGS. 1 to 4.

Example 4 Cervical Cancer Cell Cycle Analysis

Dispense HeLa cells into 6 well-plates at a density of 3 x 10 ⁵ cells / well and incubate for 24 hours (37 ° C, 5% CO ₂ ), then incubate for 24 hours with 100 μg / mL aliquot It was. Cell monolayers were washed with PBS and then cells were collected by adding trypsin-EDTA. The collected cells were stored in cold 70% ethanol for 1 hour at -20 ° C, fixed, and then stained for 30 minutes with propidium iodide (Sigma), followed by flow cytometry using FACSCalibur (Becton Dickinson, USA). The cell cycle was analyzed by flow cytometry.

Example 5 Cervical Cancer Cell Death Analysis

After dispensing the cells into 6 well-plates, 100 μg / mL of the 1000-million cactus extract was incubated for 24 hours in the same manner as above, and the cells were collected by washing the cell monolayer with PBS and adding trypsin-EDTA. Annexin V-FITC (BD Pharmingen, USA) was mixed with 50 μg / mL propidium iodide in a 1: 100 ratio and the cells were stained for 15 minutes in the dark, followed by flow cytometry using FACSCalibur (Becton Dickinson). Apoptotic cells were counted by flow cytometry.

In the present invention, 100% hexane (hexane) was used to extract the active ingredient of millennial cactus. The millennial cactus was ground into a mixer, homogenized, and then stirred for 24 hours by adding hexane (hexane). After waiting for about 5 hours, the supernatant was separated, filtered through an organic solvent-only filter, and the filtered liquid was placed in a steam extractor to separate hexane (hexane) component to extract only the active ingredient of millennial cactus.

Formulation Example 1 Preparation of Health Functional Food

1000 years Cactus Extract 1000 mg

(OHSt-E)

Vitamin mixture proper amount

70 μg of Vitamin A Acetate

Vitamin E 1.0 mg

Vitamin B1 0.13 mg

Vitamin B2 0.15 mg

Vitamin B6 0.5 mg

0.2 μg of vitamin B12

Vitamin C 10 mg

10 μg biotin

Nicotinic Acid 1.7 mg

50 μg folic acid

Calcium Pantothenate 0.5mg

Mineral mixture

Ferrous Sulfate 1.75 mg

Zinc Oxide 0.82 mg

Magnesium carbonate 25.3 mg

Potassium monophosphate 15 mg

Dibasic calcium phosphate 55 mg

Potassium Citrate 90 mg

Calcium Carbonate 100 mg

Magnesium chloride 24.8 mg

Although the composition ratio of the above-mentioned vitamin and mineral mixtures is mixed with a component suitable for a health food in a preferred embodiment, the compounding ratio may be arbitrarily modified, and the above ingredients may be mixed according to a general health functional food manufacturing method. Next, the granules may be prepared and used for preparing the health functional food composition according to a conventional method.

Formulation Example 2. Preparation of Health Functional Drink

1000 years Cactus Extract 1000 mg

(OHSt-E)

Citric acid 1000 mg

100 g oligosaccharides

Plum concentrate 2 g

1 g of taurine

Add 900 ml of purified water

After mixing the above components in accordance with the conventional method for preparing a health functional beverage, and stirring and heating at 85 ℃ for about 1 hour, the resulting solution is filtered and obtained in a sterilized 2 L container, sealed sterilization and refrigerated and then It is used for the manufacture of the health functional beverage composition of the invention. Although the composition ratio is a mixture of the components suitable for the preferred beverage as a preferred embodiment, the blending ratio may be arbitrarily varied according to the regional and national preferences such as the demand level, the demanding country, and the intended use.

Although specific portions of the present invention have been described in detail above, it will be apparent to those skilled in the art that these specific techniques are merely preferred embodiments, and thus the scope of the present invention is not limited thereto. It is therefore intended that the scope of the present invention be defined by the appended claims and their equivalents.

Claims (6)

A pharmaceutical composition for the prevention or treatment of cervical cancer, comprising the cheonnyeoncho cactus ethyl acetate extract as an active ingredient, and having the activity of inhibiting the growth of cervical cancer cells, delaying the cell cycle and apoptosis. delete delete A health functional food composition for preventing or improving cervical cancer, comprising the cheonnyeoncho cactus ethyl acetate extract as an active ingredient, and having growth inhibition of cervical cancer cells, cell cycle delay and apoptosis. delete delete

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