KR101146104B1 - The composition effective against wrinkles mainly comprised of ginsenoside rg3, rg6 and rg2, and the skin treatment composition - Google Patents

Abstract

PURPOSE: An agent for improving anti-wrinkling and skin elasticity is provided to enable long-term application without side effect. CONSTITUTION: An agent for improving anti-wrinkling contains 20-40 wt% of Rg3, 50-70 wt% of Rg6, and 2-10 wt% of Rg2 as a main ingredient. The agent is manufactured in the form of a skin softener, nutrition lotion, massage cream, or pack. The composition contains ginsenoside Rg3, ginsenoside Rg6, and ginsenoside Rg2 in a ratio of 2:7:1-4:5:1.

Description

[0001] The present invention relates to a skin wrinkle remedy comprising, as main components, ginsenoside algin 3, algin 6 and algin 2, and a skin wrinkle improving cosmetic comprising the same, wherein the ginsenosides Rg3, Rg6 and Rg2,

The present invention relates to a skin wrinkle improving and skin elasticity enhancing agent and a cosmetic preparation containing the same, and more particularly, to a skin wrinkle improving and skin elasticity enhancing agent maximizing the performance of blending certain components of ginsenosides in an appropriate ratio, And to cosmetics containing the same.

The composition according to the present invention contains ginsenosides Rg ₃ (structural formula 1), Rg ₆ (structural formula 2) and Rg ₂ (structural formula 3) separated from red ginseng and ginseng. The study, the three ginsenosides of the present inventors 2 in a ratio of 1 (Rg ₃ 20 ~ 40 wt%, Rg ₆ 50 ~ 70% by weight, Rg _2, 10% by weight): 7: 1 to 4: 5 The combination of fibroblast proliferation, collagen synthesis, elastin synthesis, and skin immunity enhancement were the most excellent.

The above-mentioned cosmetic compositions for improving skin wrinkles and skin elasticity and cosmetic compositions containing the same are excellent in improving the wrinkles of skin and improving elasticity, and there is no side effect to the skin. .

Skin wrinkles are the most important and natural symptom of aging skin and are often the norm when measuring the degree of aging. It is impossible to prevent skin aging. However, since skin plays an important role in appearance, various attempts have been made to suppress and slow down skin wrinkling, which is a representative symptom of skin, regardless of age, place and method.

Skin aging can be divided into endogenous aging and photoaging. While intrinsic aging has the characteristics of relatively slight fine wrinkles, dryness of the skin, and skin elasticity, photoaging has comparatively coarse and deep wrinkles and many fine wrinkles, and early onset features. The beginning of skin wrinkling starts from the aging of the dermis, and the bending of the papillary layer, which is the boundary between the epidermis and the dermis, gradually expands and the elasticity of the elastic fiber is lost and the elasticity of the elastin is changed. The folds are folded.

Methods of preventing and improving wrinkles that have been used so far include methods of supplying nutrients to the skin itself, helping skin regeneration, or eliminating environments that cause skin aging. As a cosmetic material for this purpose, alpha hydroxy acids which induce skin elasticity through induction of collagen synthesis of dermal layer by inducing a kind of chemical peeling, or antioxidant which inhibits cell aging by inhibiting free radical generation , Coenzyme Q, Polypodium Leucotomos, Pycnogenol, and Vitamin A (retinol) have been used as antioxidants. Also, products containing vegetable extracts such as Ferulic aicd and Grape seed extracts or moisturizers such as hyaluronic acid have been used representatively.

However, products containing these ingredients are not only sensitive to changes over time, but also involve significant skin irritation when they penetrate or decompose into the skin above effective concentrations. Therefore, it is necessary to develop new raw materials that can provide more fundamental help to skin aging and satisfy drug safety and skin safety.

Because of this, plant-derived extracts and ingredients are in the spotlight as the largest category in the herbal cosmetics market. Among them, cosmetics using ginseng extract have been developed, but there are many factors that can develop single functional ingredients or complex ingredients separated from ginseng as functional cosmetics in the future.

Panax Ginseng CA Meyer is a perennial herbaceous plant belonging to the genus Araliaceae, which is a representative medicinal plant widely known and used not only in Oriental medicine but also as a folk remedy. Ginseng contains 60% ~ 70% carbohydrates such as starch, and contains components such as triterpenoid saponins, polyacetylenes, phenolic compounds, polysaccharides and peptidoglycans. It also contains proteins, peptides, alkaloids, essential oils and phytosterols, amino acids, nucleic acids, vitamins, lignans, and minerals.

 In general, the physiological activity of ginseng is caused by saponin, polysaccharides and peptides, and many studies have been conducted centering on ginsenoside. Thus, central nervous system regulation, memory, improvement of learning efficacy and anti- Since various pharmacological actions such as cancer cell proliferation and transfer inhibition action, antioxidation action, antidiabetic action, liver function improvement, radiation defense action and inhibition of thrombogenesis have been scientifically proved, the demand for medicines and functional supplements Is increasing.

It has been known that ginsenosides differ in their pharmacological action depending on the type and type of glycoside bond, and they act individually or in combination depending on their chemical structure, thus affecting various physiological functions.

The effects of ginsenoside on the skin include reduction of skin cell death by ultraviolet rays and protection of keratinocytes by ultraviolet rays ( J. Invest. Dermatol. 121: 607-613, 2003), prevention of wrinkle formation by oral administration ( J. Ginseng Res. 31: 86-92, 2007), prevention of keratinization ( Korean J. Dermatol . 28 (4): 434-440, 1990), acne prevention and treatment ( Korean J. Dermatol. 14 (4): 335-339 , 1976), increased skin elasticity and hydratability ( Fitoterapia 57 (1): 15-28, 1986), Fitoterapia 57 (4): 217-222, 1986), inhibition of collagen degradation ( Mol. 476-483, 1999), whitening effect ( Journal of the Society of Cosmetic of Korea, 27 (2) 45-56, 2001).

In particular, ginsenoside Rg ₃ has been reported to rapidly restore potent anti-cancer, anti-cancer, anti-immune, anti-cancer, and post-operative immunity. In addition, platelet aggregation inhibition and anti- , And anti-cancer drug resistance-inhibiting action. Skin-related efficacy increases the synthesis of the major components of the extracellular matrix, such as collagen and fibronectin, in skin fibroblasts, controlling the AP-1 and TGF-β1 expression of influencing the decomposition of the component (J. Soc Cosmet Scientists Korea 30 ( 2):... 2521-2525, 2004) , and also contains a large amount of ginsenoside Rg ₃ (Korean Academy of Industrial Science, 11 (9): 3325-3329, 2010), which is effective for improving skin wrinkles. Also, it was confirmed that ginsenoside Rg ₃ had a skin improving effect, and a cosmetic composition containing a single ginsenoside Rg ₃ was patented (No. 2003-65882).

Ginsenoside Rg is ₆ (3β, 2α, 12β) -trihydroxydammar -20 (21), 24-diene-6-O-α-L-rhamnopyranosyl (1 → 2) , wherein as -β-D-glucopyranoside and complement activation It was studying the pharmacological effects associated with platelet aggregation patent on a cosmetic for improving wrinkles associated with ginsenosides Rg ₆ is not filed on the bar.

Ginsenoside Rg ₂ of ginseng has been reported to have the effect of protecting the damaged skin cells against DNA recovery ( Naunyn-Schmied Arch pharmacol , 382: 89-101, 2010), and that ginsenoside Rg2 A cosmetic composition for skin protection comprising as a main component has been patented (No. 10-2005-0088741).

As described above, ginsenoside Rg ₃ and ginsenoside Rg ₂ have been studied and developed in relation to skin aging and improvement. However, when the ginsenosides were separated from red ginseng, the extraction yields of Rg ₂ and Rg ₃ were very low and their utilization as a cosmetic material was low because they were expensive compared with other chemical materials as natural materials. Also, when purely separated ginsenoside Rg ₂ and ginsenoside Rg ₃ are used as cosmetic compositions or cosmetics containing the same, there are cases in which they are respectively used. However, when they are contained in a specific ratio, There was no specific case for this.

In short, the pharmacological effects of various single components of ginsenoside have already been proven by scientific results, and many developments have already been made with regard to cosmetic compositions and cosmetics containing them. Nevertheless, the research on the complex action of ginsenosides is insignificant, and very little development has been done on them. Cosmetics and cosmetics including conventional ginosenoids have been developed with a comprehensive purpose for a wide range of functions such as ultraviolet ray blocking, whitening, moisturizing or skin-friendly functions. Therefore, I could not detect the advantage differentiated from the cosmetic. In addition, cosmetic compositions and other cosmetics containing Rg ₂ and Ginsenoside Rg ₃ , which have already been developed with cosmetic compositions, have been developed.

SUMMARY OF THE INVENTION The present invention has been made in view of the above circumstances, and it is an object of the present invention to overcome the limitations of the prior art and effectively formulate a specific component of ginsenoside derived from red ginseng and ginseng to improve skin wrinkles and skin elasticity And the like.

The present inventors have developed an excellent cosmetic preparation effective for improving skin wrinkles and elasticity and at the same time, in order to further maximize the effect, various ginsenosides were blended and their proportions were adjusted. As a result, the effects of fibroblast proliferation effect, collagen synthesis effect , Elastin synthesis effect, skin immunity enhancement effect and the like, and the content ratio of each component was confirmed.

The present invention having the above object is characterized in that it is a skin wrinkle improving agent comprising, as a main component, ginsenoside Rg ₃ , ginsenoside Rg ₆ and ginsenoside Rg ₂ .

Wherein the content of each ginsenoside is 20 to 40% by weight of Rg ₃ , 50 to 70% by weight of Rg ₆ , and 10% by weight of Rg ₂ .

The present invention is also characterized in that it is a skin wrinkle-improving cosmetic preparation containing the skin wrinkle-improving agent.

The skin wrinkle-improving cosmetic agent is any one of a softening agent, a nutritional lotion, a massage cream, or a pack.

In addition, the skin wrinkle-improving cosmetic of the present invention is characterized in that ginsenoside Rg ₃ , ginsenoside Rg ₆ and ginsenoside Rg ₂ are contained in the total cosmetics in an amount of 0.0001 to 20.0% by weight as solids.

A composition comprising the active ingredients ginsenoside Rg ₃ , ginsenoside Rg ₆ and ginsenoside Rg ₂ according to the present invention can be used alone, in combination with ginsenoside Rg ₃ , ginsenoside Rg ₆ and ginsenoside Rg ₂ The skin wrinkle improvement and the elasticity enhancement effect are superior. Further, when they are blended with 2: 7: 1 to 4: 5: 1 (20 to 40% by weight of Rg ₃ , 50 to 70% by weight of Rg ₆ and 10% by weight of Rg ₂ )

In addition, the ginsenoside Rg ₃ , ginsenoside Rg ₆ and ginsenoside Rg ₂ according to the present invention are natural extracts and have no adverse effects compared to other synthetic single raw materials. In fact, It can be applied to the skin by being manufactured with cosmetics which are proved to be absent and applied to the skin for a long period of time.

Rg _3, Rg _6, and Rg _2, respectively _, at the time of development of an extraction process and a processing process in which the composition ratio of ginsenosides Rg _3, Rg _6, and Rg ₂ satisfies the above-mentioned excellent range of effects, It is possible to reduce the economical burden because it is possible to use it as an industrial material.

Hereinafter, the present invention will be described in detail.

The cosmetic agent characterized by containing ginsenoside Rg ₃ , ginsenoside Rg ₆ and ginsenoside Rg ₂ as active ingredients according to the present invention in a ratio of 2: 7: 1 to 4: 5: 1, It can be used as a cosmetic composition that exhibits an effect of improving wrinkles and improving elasticity.

The amount of the cosmetic preparation containing the ginsenoside Rg ₃ , ginsenoside Rg ₆ and ginsenoside Rg _{2 according} to the present invention applied to the human skin can be appropriately selected according to the condition of the skin, To be in the range of 0.0001% to 12.0% by mass as a solid content. If the above composition is less than 0.0001%, the effect is less likely to be exhibited, and if it exceeds 12%, if the effect of increasing the effect is less than the application amount, the formulation of the product becomes unstable.

The composition of the present invention can be applied to the skin by preparing it as a lotion (skin), a nutritional lotion (lotion), a nutritional cream, a massage cream, an essence or a pack in a conventional manner.

When the cosmetic is manufactured by such a formulation, the other ingredients are not particularly limited and can be freely selected by a conventional method.

It can also be used in combination with various substances (or medicines) which are effective for the ultraviolet blocking effect (prevention of aging of the skin), maintenance of cell activity (anti-aging of the skin), whitening effect, antioxidation, moisturization and prevention of skin roughness.

When the active ingredient of the composition according to the present invention is formulated at a ratio of 2: 7: 1 to 4: 5: 1 of ginsenoside Rg ₃ , ginsenoside Rg ₆ and ginsenoside Rg ₂ , And it can be safely used because there is no side effect as evidenced by experimental results to be described later.

The mixture ratio of ginsenoside Rg ₃ , ginsenoside Rg ₆ and ginsenoside Rg ₂ is 2: 7: 1 (20 to 40% by weight of Rg ₃ , 50 to 70% by weight of Rg ₆ and 10% by weight of Rg ₂ ) When the concentration of ginsenoside Rg ₆ is more than 70% by weight, the effect of improving skin wrinkles and elasticity is not sufficient, and when it is less than 50% by weight, the effect is not sufficiently increased.

The present invention is explained in more detail by the following examples and experimental examples, but the present invention is not limited thereto in any way.

[Ginsenoside Rg ₃ , Ginsenoside Rg ₆ And ginsenoside Rg ₂ Gt;

(1) Preparation of ginsenoside Rg ₃

The ginseng or red ginseng was washed with purified water, dried and crushed into small pieces, and then extracted with 10 times the dry weight of ethanol as a solvent. Then, the ginseng crude saponin was separated from the obtained extract by a conventional method. 10 g of crude saponin was dissolved in 35 ml of 50% acetic acid and heated for 2 hours at 70 캜 with stirring, and then the reaction mixture was cooled to room temperature. The reaction solution was diluted to 300 ml with distilled water and extracted three times with 150 ml of n-butanol. The combined extracts were washed twice with saturated sodium bicarbonate solution and the n-butanol layer was concentrated under reduced pressure. The reaction mixture was separated by silica gel column chromatography (chloroform: ethanol: water = 100: 30: 10, lower layer) to obtain ginsenoside Rg ₃ .

(2) Preparation of ginsenoside Rg ₆

The ginseng or red ginseng was washed with purified water, dried and crushed into small pieces, and then extracted with 10 times the dry weight of ethanol as a solvent. Then, the ginseng crude saponin was separated from the obtained extract by a conventional method. Ginsenoside Rg ₆ was isolated from the separated crude saponin using a Purp Elsy (Waters Co., Ltd., Delta prep 4000, USA). The separated ginsenoside Rg ₆ was qualitatively analyzed by HPLC (Waters 2690 Seperations Module, Waters 2487 Dual λ Absorbance Detector, USA) to confirm that the purity was 96% or more.

(3) Production of ginsenoside Rg ₂

The ginseng or red ginseng was washed with purified water, dried and crushed into small pieces, and then extracted with 10 times the dry weight of ethanol as a solvent. Then, the ginseng crude saponin was separated from the obtained extract by a conventional method. 10 g of crude saponin was dissolved in 35 ml of 50% acetic acid, and the mixture was heated under reflux for 3 hours and then concentrated under reduced pressure to obtain about 5 g. The reaction product was dissolved in distilled water and separated into a column packed with an alkylbenzene-based porous resin to obtain ginsenoside Rg ₂ .

[Experimental Example 1] Measurement of fibroblast proliferation effect

The human skin fibroblast CCD986sk was dispensed into each well of a 96-well microplate at 5 × 10 ³ cells / well and cultured in DMEM containing fetal bovine serum Dulveccos Modified Eagles Media) for 24 hours at 37 < 0 > C. Retinol was treated as a positive control and 100 μl of the mixture of prepared ginsenosides Rg ₃ , Rg ₆ , Rg ₂ and ginsenoside Rg ₃ / Rg ₆ / Rg _{2 was} added to the culture medium. The control group was supplemented with a basal diet not treated with ginsenosides Rg ₃ , Rg ₆ , Rg ₂ and a mixture of ginsenosides Rg ₃ / Rg ₆ / Rg ₂ . Then, the cells were cultured in a CO ₂ (5%) incubator at 37 ° C for 72 hours, and a solution of 0.2% MTT (3- (4,5-dimethylthiazol-2-yl) -2,5-diphenyltetrazolium bromide) 50 μl was added to each well and allowed to stand for 4 hours. After removing the supernatant, 150 μl of DMSO (dimethyl sulfoxide) was added and formazan was dissolved by shaking for 10 minutes. The absorbance of formazan dissolved in DMSO was measured at 570 nm using a microplate reader. This was compared with the absorbance of the control group to show the fibroblast proliferation effect.

Cell proliferation effect (%) = (absorbance of experimental group-absorbance of control group) / absorbance of control group * 100

As a result of the experiment, fig. 1, the positive control group, retinol, exhibited cell proliferation of about 90%, and about 96 to 101% of ginsenoside Rg ₃ , ginsenoside Rg ₆ and ginsenoside Rg ₂ alone, Of fibroblast proliferation. Ginsenoside Rg ₃ , ginsenoside Rg ₆ and ginsenoside Rg ₂ at a ratio of 2: 7: 1 to 4: 5: 1 to exhibit a fibroblast proliferation effect of about 152 to 165% It can be confirmed that the effect is specifically increased in the treatment.

Fig. 1. Graph showing the fibroblast proliferation effect of Rg ₃ / Rg ₆ / Rg ₂ mixture according to the present invention

[Experimental Example 2] Activity inhibition of MMPs (Matrix metalloproteinases)

Prepared ginsenoside Rg _3, Rg _6, Rg ₂ and ginsenoside Rg ₃ / Rg ₆ / Rg ₂ mixture and a skin fibroblast describing the inhibitory effect of Matrix metalloproteinases by the retinol positive control group in Experiment Example 1 (human skin fibroblast).

In order to measure the activity of MMPs, gelatin zymography was performed. CCD986sk culture was cultured under the conditions described in Experimental Example 1, and centrifuged at 400 xg to obtain supernatant. Then, 2X sample buffer (Invitrogen Co., USA) Youngdong samples were prepared. The gel was electrophoresed using a gel containing 0.1% gelatin added to 10% polyacrylamide, and the gel was renaturated with 2.5% Trino X-100 (Sigma Co., Ltd USA) for 30 minutes, (50 mM Tris-HCl, 150 mM NaCl, 50 mM CaCl 2, 1 μM ZnCl 2, 0.02% NaN 3, pH 7.5). Developing buffer was removed and the gel was stained with 0.5% (w / v) Coomassie brilliant blue G-250 (Bio-Rad Laboratories, USA) for 1 hour and decolorized with methanol: acetic acid: water : 6)) to confirm the extent of gelatin degradation.

MMPs inhibition rate (%) = (1-B / A) x 100

(A: absorbance of the control group, B: absorbance of the test substance-treated cell group)

MMPs are degrading factors that inhibit collagen synthesis. Inhibition of MMPs activity will soon reduce collagen degradation, delaying the maintenance of skin elasticity and wrinkling. fig. 2, ginsenoside Rg ₃ , ginsenoside Rg ₆ and ginsenoside Rg ₂ In the case of each treatment alone, the effect of MMPs was observed to be about 26%, while the effect was increased to about 36% or more when mixed in a specific ratio. Especially, 2: 7: 1 to 4: 5: 1 showed higher inhibition of MMPs than retinol.

[Fig. 2] Activity inhibition of MMPs

[Experimental Example 3] Measurement of collagen synthesis amount

The mixture of ginsenosides Rg ₃ , Rg ₆ , Rg ₂ and ginsenoside Rg ₃ / Rg ₆ / Rg ₂ was dissolved in PBS buffer at a proper concentration and used in the experiment. The control group was supplemented with the basic medium, and the positive control group was retinol Respectively. The culture medium of CCD986sk cell line cultured under the conditions described in Experimental Example 1 was replaced with DMEM (Dulveccos Modified Eagles Media) supplemented with no serum. Then, the extract was added to CCD986sk cell culture plate to a constant concentration and cultured for 24 hours, 48 hours Lt; / RTI > After culturing for a given period of time, each cell culture solution was collected and the amount of procollagen type I C-peptide present in the culture solution collected using a collagen protein measurement kit (Takara Shuzo Co., Ltd., Japan) Production increase effect was investigated. To this end, the primary collagen antibody was coated on a 96-well microplate, and each collected CCD986sk cell culture was added and reacted at 37 ° C for 3 hours. After removing the cell culture medium, the cells were washed three times with PBS buffer solution. The reaction was stopped by addition of 1 N sulfuric acid solution, and the absorbance at 450 nm was measured using an ELISA reader. The absorbance value obtained by the above method was substituted into the standard curve obtained from the standard solution, and the amount of collagen production in the CCD986sk cell culture fluid to which each sample was added was calculated. Collagen production synthesis rate was obtained from the following equation.

Collagen production synthesis rate (%) = [(Ax100) / B] -100

(A: collagen production amount of the culture solution to which the sample is added, and B: collagen production amount of the culture solution to which no sample is added)

Collagen, which is the main constituent of the skin, is present at high concentrations in skin, bone, ligament, cartilage and tooth, and it has mechanical strength of skin, resistance of connective tissue and binding force of tissue, support of cell adhesion, induction of cell division and differentiation And so on. It is also reduced by aging and photoaging by ultraviolet irradiation, which is closely related to the wrinkling of the skin. Therefore, the discovery of a material that promotes collagen synthesis is very useful as a raw material for cosmetics.

fig. 3, the effect of ginsenoside Rg ₆ alone was promoted by about 37%, whereas the effect of ginsenoside Rg ₆ was decreased by 50% (w / w) or less. The mixed treatment of ginsenoside Rg ₃ , ginsenoside Rg ₆ and ginsenoside Rg ₂ resulted in a maximum of about 70% at a composition ratio of 2: 7: 1 to 4: 5: 1, compared to the positive control retinol It was confirmed that the effect was increased. Therefore, in the effect of promoting collagen synthesis, mixing of ginsenoside Rg ₃ , ginsenoside Rg _6, and ginsenoside Rg ₂ at a ratio of 2: 7: 1 to 4: 5: 1, .

[Fig. 3] Results of measurement of collagen synthesis amount

[Experimental Example 4] Elastase activity inhibition

The prepared ginsenosides Rg ₃ , Rg ₆ , Rg ₂ and ginsenoside Rg ₃ / Rg ₆ / Rg ₂ The inhibitory effect of the mixture on the elastase activity was examined using porcine pancreas elastase (Sigma Co., Ltd., USA) and N-succinyl- (L-Ala) 3-p-nitroanilide USA). ≪ / RTI > Rinsing ginsenosides Rg ₃ , Rg ₆ , Rg ₂ And ginsenoside Rg _3/6 Rg / Rg ₂ 0.5 ml of the mixture solution was added to the test tube and 0.5 ml of porcine pancreas elastase (1 U / ml) solution dissolved in 100 mM Tris-HCl buffer solution (pH 8.0) was added thereto and dissolved in DMSO to prepare 3.2 μM N-succinyl- 3-p-nitroanilide (0.1 ml) was added thereto, followed by reaction at room temperature for 20 minutes. Elastase activity was determined by measuring the amount of ρ-nitroanilide produced from the substrate using a spectrophotometer at 410 nm.

Elastase inhibition rate (%) = (1-B / A) x 100

(A: absorbance of the control group, B: absorbance of the test substance-treated cell group)

Collagen and elastin in the dermal tissue of the skin form a network structure and maintain the elasticity of the skin. However, due to the internal and external stresses such as age and ultraviolet rays, the elasticity and the elasticity decrease, and when the elastin network structure is broken by the overexpressed elastase, the skin becomes wrinkled and wrinkles occur.

fig. As shown in Fig. 4, treatment with ginsenoside Rg ₆ alone showed a relatively high elastase inhibitory effect of about 51%. Also, when the ginsenosides Rg ₃ / Rg ₆ / Rg ₂ were mixed, it was confirmed that the effect was greatly increased to about 82% at a composition ratio of 2: 7: 1 to 4: 5: 1. However, 5: 4: If the composition ratio of more than 1, that ginsenoside Rg ₆ to about 50% (w / w) following process can be seen that the effect is gradually decreased.

As a result of measuring the elastase inhibitory activity associated with wrinkle formation _, the elastase inhibitory activity was increased by treating a mixture of Rg _3, Rg ₆ and Rg ₂ when the ginsenosides Rg _3, Rg ₆ and Rg ₂ were treated alone, Especially in the ratio of 2: 7: 1 to 4: 5: 1, exhibiting the most effective elastase inhibitory activity.

[Fig. 4] Comparison of inhibitory activity of elastase activity of Rg ₃ / Rg ₆ / Rg ₂ mixture according to the present invention

[Preparation Examples 1 and 2] Softening time (skin)

The softening skin containing the ginsenosides Rg ₃ , ginsenoside Rg ₆ and ginsenoside Rg ₂ of the present invention was prepared in a conventional manner at the following proportions:

[Fig. 5] Ingredient ratio of softened longevity (unit:% by weight)

[Production Examples 3 and 4] Nutritional lotion (lotion)

Nutritional lotions containing the ginsenoside Rg ₃ , ginsenoside Rg ₆ and ginsenoside Rg ₂ of the present invention were prepared in a conventional manner at the following proportions.

[Fig. 6] Composition ratio of nutrition lotion (unit:% by weight)

[Production Examples 5 and 6] Nourishing cream

Nutrition creams containing the ginsenosides Rg ₃ , ginsenoside Rg ₆ and ginsenoside Rg ₂ of the present invention were prepared in a conventional manner at the following proportions:

[Fig. 7] Composition ratio of nutritional cream (Unit:% by weight)

[Production Examples 7 and 8]

A pack containing the ginsenoside Rg ₃ , ginsenoside Rg ₆ and ginsenoside Rg ₂ of the present invention was prepared in a conventional manner at the following proportions.

[Fig. 8] Component ratio of pack (unit:% by weight)

[Experimental Example 5] Stability test of formulation

For the formulation stability test of the cosmetic composition of the present invention, the cosmetic composition prepared in Preparation Examples 1 to 8 was placed in an opaque glazed container and kept in a constant temperature bath maintained at 45 캜 for 12 weeks. ≪ / RTI > and stored for 12 weeks in a fully shaded refrigerator and the degree of discoloration was measured. The degree of discoloration of the product was classified into the following six grades and evaluated.

0: No change, 1: Very little separation (discoloration), 2: Little separation (discoloration)

3: Severely separated (discolored), 4: Severely separated (discolored), 5: Severely separated (discolored)

[Fig. 9] Degree of discoloration of each formulation

The cosmetic composition of the present invention does not cause discoloration and separation phenomenon, and the amount of the cosmetic preparation containing the ginsenoside Rg ₃ , ginsenoside Rg ₆ and ginsenoside Rg _{2 according} to the present invention is 0.0001% to 12.0% Lt; RTI ID = 0.0 > of < / RTI > the stability of the formulation.

[Experimental Example 6] Skin stability test

In order to test skin safety of the compositions of the present invention, the following safety tests were conducted on the cosmetic compositions formulated in Preparation Examples 1 to 8.

1) Primary skin irritation test

The cosmetic preparation was applied to the skin of male white rabbits with hair on the dorsal skin removed once a day, five times a week to a thickness of 2 mg / cm 2 or more. The application was carried out for 2 weeks, and hair removal was carried out every week at the final application day. The test was performed at 24, 48, and 72 hours after application with the primary irritation test using erythema and edema as an index.

As a result of the test, it was judged to be negative because no erythema and edema were observed in all experimental groups.

2) Skin Cumulative Irritation Test

The cosmetic preparation was applied to the skin of male white rabbits with hair on the dorsal skin removed once a day, five times a week to a thickness of 2 mg / cm 2 or more. The application was carried out for 2 weeks, and hair removal was carried out every week at the final application day. The determination was carried out using the erythema and edema as the indicators by the primary irritation method on the day of each application and the day after the last application.

As a result of the test, skin irritation caused by repeated application of the preparation was not observed in all the test groups and it was judged as negative.

From the above results, it can be seen that when the ginsenosides Rg ₃ , Rg ₆ and Rg ₂ are blended according to the present invention, the skin elasticity and wrinkle can be improved, : 5: 1 ratio (Rg ₃ 20-40 wt%, Rg ₆ 50 to 70% by weight, and Rg ₂ 10% by weight).

Claims (5)

delete A skin wrinkle improving agent comprising 20 to 40% by weight of ginsenoside Rg ₃ , 50 to 70% by weight of Rg ₆ , and 10% by weight of Rg ₂ as main components. A skin wrinkle-improving cosmetic preparation containing the skin wrinkle-improving agent according to claim 2. The method of claim 3,
Wherein the skin wrinkle improving agent is any one of a softening agent, a nutritional lotion, a massage cream or a pack. The method of claim 3,
Wherein ginsenoside Rg ₃ , ginsenoside Rg ₆ and ginsenoside Rg ₂ are contained in the total cosmetics in a solid content of 0.0001 to 20.0% by weight.