KR101128477B1 - Cosmetic Composition for Acne Improvement and Method for Preparing Therof - Google Patents

Abstract

The present invention relates to a cosmetic composition for improving acne, characterized by containing the lacquer extract, the cosmetic composition has no skin irritation by the lacquer component and has the effect of inhibiting the growth of acne bacteria, and at the same time the activity of 5α-reductase It has the effect of suppressing.

Sumac extract, skin irritation, acne bacteria, 5α-reductase, cosmetic composition

Description

Cosmetic composition for acne improvement and its manufacturing method {Cosmetic Composition for Acne Improvement and Method for Preparing Therof}

The present invention relates to a cosmetic composition for improving acne containing lacquer extract and a method for producing the same. More specifically, the present invention contains an lacquer extract containing 0.5 to 3.0% by weight of camphorol glycosides, which has an anti-acne bacterium effect without irritating the skin, and also contains an lacquer extract that inhibits the activity of 5α-reductase. It relates to a cosmetic composition for improvement and a method for producing the same.

Acne refers to an inflammatory skin disease that occurs in hair follicles and sebaceous glands. The causes of such acne include an increase in sebum secretion, narrowing of hair follicles, and proliferation of acne bacteria (Propionibacterium acnes). Sex hormones that increase after puberty, especially male hormones, excessively increase the secretion of sebum.Because the lipid composition of sebum is different from the skin epidermis, it is well oxidized and is easily used as an energy source of bacteria. May lead to the growth of acne can lead to acne. The increased acne is also recognized as an antigen, causing an inflammatory response. This causes papules and worsens when the hair follicle wall is destroyed and tissues are damaged, such as cysts or pustules. To alleviate the symptoms of acne, sebum, which is the energy source of acne, should be reduced or the sebum secreted frequently should be removed. And acne bacteria actively proliferate under anaerobic conditions, so if you remove the exfoliation of skin to prevent hair follicles narrowing, or inhibit the growth of acne bacteria itself, acne symptoms can be alleviated.

 To suppress acne bacteria, antibiotics such as tetracycline, minocycline and erythromycin, external medications such as benzoyl peroxide, anti-inflammatory agents for treating inflammation, and hormone preparations for sebum control may be used, but this is a method in the pharmaceutical field.

In the cosmetic field, a method of removing dead skin cells and sterilizing acne bacteria by adding ingredients such as resorcinol, sulfur, salicylic acid and benzoyl peroxide to cosmetics has been mainly used. However, due to these ingredients, the skin is easily irritated and may cause side effects such as redness or dryness easily. Due to such side effects, the concentration of the cosmetics may be limited, and thus the acne bactericidal effect is not great. Therefore, attempts to achieve acne improvement effects using natural and safe herbal extracts have been conducted and there are no satisfactory research results so far.

On the other hand, lacquer trees are deciduous trees that live in various parts of our country. Dried lacquer can be used as a pain medicine, insect pest medicine, cough medicine, and it is also effective in skin diseases and wounds. In addition, lacquer is known to have effects of anticancer activity, antioxidant activity, hangover relief and liver function recovery activity. Nevertheless, lacquer has been extremely limited in its use because it has a property of rejection upon contact with the skin.

Accordingly, the present inventors have an antibacterial effect, in particular, an anti-acne bactericidal effect without irritating the skin, and have an effect of inhibiting the activity of 5α-reductase, a cosmetic composition capable of improving acne containing lacquer extract and its preparation It is time to develop a method.

An object of the present invention is to provide a cosmetic composition for improving acne containing lacquer extract without skin irritation.

Another object of the present invention is to provide a cosmetic composition for improving acne containing a lacquer extract having an antibacterial effect, in particular, an anti acne bacterium effect.

Another object of the present invention is to provide a cosmetic composition for improving acne containing lacquer extract having an antibacterial effect, in particular, an anti acne bacterium effect, without stimulating the skin having the effect of inhibiting the activity of 5α-reductase.

Another object of the present invention is to provide a method for producing a cosmetic composition for improving acne containing lacquer extract, which has no skin irritation, has an anti-acne bactericidal effect, and at the same time has an effect of inhibiting the activity of 5α-reductase.

The above and other objects of the present invention can be achieved by the present invention described below.

The present invention relates to a cosmetic composition for improving acne, characterized by containing the lacquer extract.

In one embodiment of the present invention, the sumac extract is characterized in that it comprises 0.5 to 3.0% by weight of camphorol glycosides.

In another embodiment of the present invention, the lacquer extract is a step of fermenting and ripening raw lacquer tree, drying the fermented and aged lacquer tree, steaming the dried lacquer with an extraction solvent to obtain an extract, and aging the extract. It may be prepared by a method comprising the step of filtering the aged extract.

In another embodiment of the present invention, the lacquer extract is an aqueous solution or water containing 0.01 to 60% by weight of one or more components selected from the group consisting of alcohol, glycerin, alkylene glycol, benzene, chloroform, hexane, acetone It can be extracted using as an extraction solvent.

In another embodiment of the present invention, the lacquer extract may be included as 0.001 to 30% (w / v) as its dry weight in the cosmetic composition for improving acne containing lacquer extract.

In order to achieve the above another technical problem, the present invention comprises the steps of fermenting and maturing the sumac; Drying the fermented and aged lacquer; Steaming the dried lacquer with an extraction solvent to obtain an extract; Aging the extract; Filtering it; And it provides a method for producing a cosmetic composition for improving acne, comprising the step of mixing the aged extract with the oily raw material.

Acne-improving cosmetic composition containing the lacquer extract according to the present invention has an antibacterial effect, in particular, prevents the growth of acne bacteria without stimulating the skin, and inhibits excessive sebum production by inhibiting the activity of 5α-reductase.

Hereinafter, the present invention will be described in more detail.

The present invention provides a cosmetic composition for improving acne, characterized by containing the lacquer extract.

The sumac extract is characterized in that it comprises 0.5 to 3.0% by weight of camphor roll glycosides. Camphorol is represented by the chemical formula of C15H10O6 and refers to one of the flavonoids having a typical C6-C3-C6 structure. The sumac extract preferably comprises 0.9 to 1.5% by weight of camphorol glycosides.

The present inventors have found that not only has an anti-acne bacterium effect on the lacquer extract, but also inhibits the activity of 5α-reductase, the most important enzyme in male hormone biosynthesis. Inhibiting the activity of 5α-reductase inhibits male hormone biosynthesis and, consequently, excessive sebum production. That is, it is possible to obtain an effect of blocking sebum production, which is one of the most important factors for puberty acne production. The present inventors have come to invent a cosmetic composition for improving acne using these new properties of the sumac extract.

In one embodiment, the lacquer extract is a step of fermenting and ripening raw lacquer, drying the fermented and aged lacquer, obtaining dried extracts by extracting the dried lacquer with an extraction solvent, aging the extract, aging It can be prepared by a method comprising the step of filtering the extract.

Fermentation and ripening of raw lacquer can be done by soaking in alcohol for 5-30 days. It is preferable to use the alcohol contained in the range of 1% to 50% of the concentration, and edible alcohols such as makgeolli and shochu may be preferably used. Fermentation and maturation of raw lacquer occurs over 10 to 30 days, but if the period is shorter than 1 day, fermentation and ripening does not occur sufficiently, and if fermentation is more than 30 days, there is a risk of excessive time waste and fermentation. If not sufficiently fermented and aged for more than 1 day, the finally obtained lacquer extract may cause irritation to the skin, so a sufficient fermentation and ripening process is very important. Preferably fermented and aged fermented lacquer for 13 to 17 days is good. Since the fermentation and aging process is sufficient as described above, it is possible to obtain a lacquer extract without skin irritation that can be used for cosmetic purposes.

The drying of fermented and aged lacquer is not particularly limited in its drying method, but it is preferable to leave it for 8 hours or more in the shade to avoid direct sunlight and to dry it.

In the step of obtaining the extract by drying the dried lacquer with an extraction solvent, water is used as the extraction solvent, or at least one component selected from the group consisting of alcohol, glycerin, alkylene glycol, benzene, chloroform, hexane, acetone 0.01 An aqueous solution containing from 60% by weight is used. The steaming time with the extraction solvent is 1 hour or more, preferably 8 to 10 hours.

The extract obtained by steaming with the extraction solvent is subjected to a step of aging for 1 to 30 days. If the period is shorter than 1 day, ripening will not occur sufficiently, and if fermented for 30 days or more, there is a risk of corruption. Preferably aged for 13 to 17 days.

Filtering the aged extract to separate the solids to produce a lacquer extract that can be used as a raw material of cosmetics, and when the extract is mixed with an oily material cosmetic composition for improving acne that can be useful in the formulation of various cosmetics Is obtained.

The sumac extract of the present invention may be prepared by other methods than the method described in detail above. For example, a method may be employed in which a poison lacquer is heated to remove toxicity and a extract is extracted from the heat treated lacquer. As such, the extraction method of the extract for removing the toxicity of lacquer can be easily selected and used by those skilled in the art, regardless of the extraction method, acne using the anti-acne bacterium effect, 5α-reductase activity inhibitory effect of the lacquer extract All cosmetic compositions for improvement belong to the scope of the present invention.

The sumac extract may be included as 0.001 to 30% (w / v) as the dry weight of the sumac extract-containing acne improvement cosmetic composition. In other words, the lacquer extract with respect to 100 ml of the total composition solution may be included in a ratio of 0.001 ~ 30g as its dry weight. If the content is more than 30% (w / v), the active ingredient may be excessive skin irritation occurs, while if the content is less than 0.001% (w / v) acne improvement may be insignificant.

The cosmetic composition for improving acne may further include an oily material. Oily raw material is a base added for moisturizing action, which is a basic function of cosmetics, specifically, glycerin, butylene glycol, propylene glycol, higher alcohols, fatty acid esters, triglycerides, ethylene oxide, propylene oxide, ceramide, triethanol amine, cytosterol, Castor oil, Yaja oil, Olive oil, Carnauba wax, Candelilla nap, Soybean oil, Cottonseed oil, Avocado oil, Cholesterol, Lanolin, Beeswax, Squaline, Uji, Whale oil, Egg yolk oil, Tador oil, Liquid paraffin, Solid paraffin, Wased Lean, microcrystalline wax, selesin, and the like may be used, but are not limited thereto. The oily material may be easily selected and used by those skilled in the art according to the use of the cosmetic composition.

The cosmetic composition for improving acne may further include a surfactant. Surfactants are for emulsifying, solubilizing, penetrating, specifically, higher alcohol phosphate esters, higher alcohol sulfate ester salts, anionic surfactants such as higher fatty acid soaps, cationic surfactants such as quaternary ammonium salts, nonionic surfactants, and amphoterics. Surfactants and the like may be used, but are not limited thereto. The surfactant may be easily selected and used by those skilled in the art according to the use of the cosmetic composition.

The cosmetic composition for improving acne may be an antiseptic, colorant, flavoring agent, antioxidant, astringent, deodorant, antihistamine, hormone, metal ion sequestrant, rubbery, starch, polymer compound, wetting agent, powder, and sweating depending on the purpose of the final cosmetic. It may further comprise an inhibitor and other additives, the appropriate selection of one or more of the additives may be easily carried out by those of ordinary skill in the art belonging to the present invention.

Example

1. Preparation of sumac extract and measurement of antimicrobial effect

Example 1

5 Kg of bark of raw lacquer was crushed to a length of 10 cm, then immersed in makgeolli and left at 4 ° C. for 15 days to ferment and mature the raw lacquer. Thereafter, the resultant was dried using a sieve, which was left to dry in the shade for 8 hours to avoid direct sunlight, and the dried product was triturated for 8 hours using 5 Kg of purified water as an extraction solvent to obtain an extract. The extract thus obtained was aged at 4 ° C. for 15 days and filtered to prepare a lacquer extract. The final yield of the sumac extract thus obtained was 400 ml.

1 ml of the lacquer extract prepared as described above was dissolved in 9 ml of a 1,3-butylene glycol aqueous solution, and 30 µl of the solution was added to a 5 mm diameter paper disk, and the bacteria were treated under the following conditions. Incubated. Table 1 shows the results of measuring the diameters of the clear zones appearing around the paper disks after incubation.

Candida albicans (ATCC 10259): The nutrient agar medium was used and incubated for 48 hours at 37 ° C., aerobic conditions.

Escherichia coli (ATCC 25922): Tryptic soy agar was used as a medium and incubated for 48 hours at 37 ° C., aerobic conditions.

-Streptococcus aureus (Staphylococcus aureus, ATCC 29737): The nutrient agar medium was used and incubated for 48 hours at 37 ℃, aerobic conditions.

Bacillus subtilis (ATCC 6633): Agar agar medium and incubated for 48 hours at 37 ℃, aerobic conditions.

Streptococcus mutans (ATCC 25175): Brain heart infusion agar was used as a medium and incubated for 24 hours at 37 ° C., aerobic conditions.

Staphylococcus epidermidis (ATCC 12228): Agar agar medium was used and incubated for 48 hours at 37 ° C., aerobic conditions.

-Acne (Propionibacterium acne, ATCC 6919): Reinforced clostridial agar (reinforced clostridial agar) was used as a medium and incubated for 72 hours at 37 ℃, anaerobic conditions.

Examples 2 to 13

A lacquer extract was prepared in the same manner as in Example 1, except that the following solvent was used as the extraction solvent. Final yields of the extraction solvent and lacquer extract used in Examples 2 to 13 are as follows.

              Extraction solvent final yield (ml)

Example 2 30% Ethanol 348

Example 3 50% Ethanol 321

Example 4 30% n-propanol 410

Example 5 30% Isopropanol 427

Example 6 30% n-butanol 389

Example 7 30% Glycerin 405

Example 8 30% Propylene Glycol 394

Example 9 30% Butylene Glycol 391

Example 10 30% Benzene 355

Example 11 30% Chloroform 370

Example 12 30% Hexane 396

Example 13 30% Acetone 343

Using each of the lacquer extract of Examples 2 to 13 prepared as described above to incubate each bacterium in the same manner as in Example 1, after measuring the diameter of the clear zone appearing around the paper disk after the culture Is shown in Table 1.

Comparative Example 1

Instead of the lacquer extract, 1 ml of distilled water was dissolved in 9 ml of 1,3-butylene glycol aqueous solution, and 30 µl of the solution was dropped onto a 5 mm diameter paper disk and incubated in the same manner as in Example 1. Table 1 shows the results of measuring the diameters of the clear zones appearing around the paper disks after incubation.

[Table 1]

sample
Use strain
Candida Albican
Escherichia coli
Streptococcus aureus
Bacillus subtilis
Streptococcus mutans
Steffilo Caucus Epidermidis
Acne Bacteria
Example 1
21
28
24
24
26
21
25
Example 2
19
21
24
22
27
21
25
Example 3
19
23
23
22
21
28
25
Example 4
18
27
23
22
25
22
24
Example 5
19
25
25
24
22
22
26
Example 6
21
28
29
23
21
29
26
Example 7
22
26
28
23
21
28
27
Example 8
31
25
28
26
28
29
25
Example 9
18
21
28
21
26
27
26
Example 10
22
22
25
21
26
27
26
Example 11
21
23
25
25
28
25
23
Example 12
18
24
26
26
28
26
25
Example 13
21
24
26
21
26
25
22
Comparative Example 1
0
0
0
0
0
0
0

                                                          (Unit: mm)

Looking at the results of Table 1, Examples 1 to 13 all have antimicrobial activity, and it can be seen that there is an excellent effect, especially for acne bacteria closely related to the present invention. On the other hand, the comparative example in which distilled water was added instead of the lacquer extract showed no antibacterial activity.

The antimicrobial activity of Example 8 was the best, and in the following, a cosmetic composition for improving acne containing lacquer extract was prepared using Example 8.

2. Determination of 5α-reductase Enzyme Inhibitory Effect of Rhus verniciflua Extract

5α-reductase is the most important enzyme in male hormone biosynthesis and is the most important factor in puberty acne production through excess sebum production. By measuring the inhibitory effect of the test substance on 5α-reductase, the indirect effects on sebum production inhibition and acne inhibition can be identified. Treating the HEK293 (Human Embryonic Kidney) cell line, a human-derived 5α-reductase secretion cell, with the lacquer extract of Example 8 to determine whether the secretion of 5α-reductase was inhibited by the treatment of the lacquer extract of Example 8 Measured. HEK293 cell lines, 5α-reductase secreting cells, were cultured at 37 ° C. and 5% CO 2 with medium containing 10% fetal bovine serum to RPMI 1640. After culturing the cells at a concentration of 106 cells / well in a 24-well plate and confirming the cell attachment, the test substance (lacquer extract of Example 8) was added to the cell culture solution by concentration to incubate for 24 hours. It was. The culture medium was recovered, and according to the method of Baerbel P. et al. (Mol Cell Biochem 270: 201-208, 2005), the substrate 4-androstene-3,17-dione was reacted with 5α-androstane-3, by the action of 5α-reductase. The rate of change to 17-dione was measured. According to the following formula, 5α-reductase enzyme activity inhibition rate was calculated, and the result is shown in Table 2.

 Inhibition rate of enzyme activity (%) = 100- (production amount in the presence of each test substance / substrate change amount of control group X 100)

TABLE 2 Inhibition rate of reductase

Test substance (treatment concentration) Enzyme Inhibition Rate (%) Extract 1.0%  12.4 Extract 2.0% 26.8 Extract 3.0%  40.9 Control group 0

As can be seen in Table 2, the lacquer extract of the present invention concentration-dependently inhibit 5α-reductase activity, an enzyme important for sebum production.

3. Analysis of flavone glycoside content

Kaempferol glycoside content of the lacquer extract of Example 8 was measured using a standard kaempferol. The results are shown in Table 3 below.

[Table 3]

Number of experiments Kempferol Glycoside Content (% by weight) Example 8-Once 1.03 Example 8-2 Times 0.98 Example 8-3 Times 1.05 Average 1.02

Looking at the above test results, it can be seen that the lacquer extract of the present invention is particularly excellent in acne improvement when the content of the camphor roll glycoside is more than 0.90% by weight.

4. Acne improver containing lacquer extract Cosmetics  Preparation of the composition and measurement of skin irritation

(1) Preparation of cosmetic composition for acne improvement

The unit of numerical values used in Tables 4 to 8 is% (w / v).
Formulation Example 1

Formulation Example 1 relates to a lotion (skin lotion) in the formulation of the cosmetic composition for improving acne containing lacquer extract, it was prepared as shown in Table 4. Here, the lacquer extract is that of Example 8.

[Table 4]

number
Raw material
Formulation Example 1
Comparative Formulation Example 1
One
Sumac extract (Example 8)
10.0
-
2
glycerin
3.0
3.0
3
Butylene Glycol
2.0
2.0
4
Propylene glycol
2.0
2.0
5
Polyoxyethylene (60) Cured Castor Oil
1.00
1.0
6
ethanol
10.0
10.0
7
Triethanolamine
0.1
0.1
8
antiseptic
a very small amount
a very small amount
9
Pigment
a very small amount
a very small amount
10
Spices
a very small amount
a very small amount
11
Purified water
Balance
Balance

11, 2, 3, 4, and 8 were added in order to dissolve and then dissolved. 5 was heated to 60 ° C. for dissolution and 10 times were added to 11 to add and stir. Finally, 1,6,7,9 was added to the mixture and stirred sufficiently, and then passed through a mitrofluidizer and aged.

Formulation Example 2

Formulation Example 2 As related to nutrition lotion in the formulation of the cosmetic composition for improving acne-containing lacquer extract, it was prepared as shown in Table 5. The sumac extract here is that of Example 8.

TABLE 5

number
Raw material
Formulation Example 2
Comparative Formulation Example 2
One
Sumac extract (Example 8)
10.0
-
2
Cytostolol
1.70
1.70
3
Polyglyceryl 2-oleate
1.50
1.50
4
Ceramide
0.7
0.7
5
Ceteares-4
1.2
1.2
6
cholesterol
1.5
1.5
7
Dicetylphosphate
0.4
0.4
8
Concentrated glycerin
5.0
5.0
9
Sunflower oil
10.0
10.0
10
Carboxy Vinyl Polymer
0.2
0.2
11
Xanthan Gum
0.3
0.3
12
antiseptic
a very small amount
a very small amount
13
Spices
a very small amount
a very small amount
14
Purified water
Balance
Balance

Non-ionic amphiphilic lipids were prepared by homogenizing the raw materials 2, 3, 4, 5 and 6 at a constant temperature. The nonionic amphiphilic lipid and the raw materials 1,7,8 and 14 are mixed and homogenized at a constant temperature to pass through a microfluidizer, and then the raw material 9 is gradually added at a constant temperature to homogenize and then microfluda again. Passed it through again. 10, 11, 12, 13 was added and dispersed to stabilize and mature.

Formulation Example 3

Formulation Example 3 relates to the nutritional cream of the formulation of the cosmetic composition for improving acne containing lacquer extract, it was prepared as shown in Table 6. Here, the lacquer extract is that of Example 8.

TABLE 6

number
Raw material
Formulation Example 3
Comparative Formulation Example 3
One
Sumac extract (Example 8)
10.0
-
2
Cytostolol
4.0
4.0
3
Polyglyceryl 2-oleate
3.0
3.0
4
Ceramide
0.7
0.7
5
Ceteares-4
2.0
2.0
6
cholesterol
3.0
3.0
7
Dicetylphosphate
0.4
0.4
8
Concentrated glycerin
5.0
5.0
9
Sunflower oil
22.0
22.0
10
Carboxy Vinyl Polymer
0.5
0.5
11
Triethanolamine
0.5
0.5
12
antiseptic
a very small amount
a very small amount
13
Spices
a very small amount
a very small amount
14
Purified water
Balance
Balance

Non-ionic amphiphilic lipids were prepared by homogenizing the raw materials 2, 3, 4, 5 and 6 at a constant temperature. The nonionic amphiphilic lipid and the raw materials 1,7,8 and 14 are mixed and homogenized at a constant temperature to pass through a microfluidizer, and then the raw material 9 is gradually added at a constant temperature to homogenize and then microfluda again. Passed it through again. 10, 11, 12, 13 was added and dispersed to stabilize and mature.

Formulation Example 4

Formulation Example 4 relates to the massage cream in the formulation of the cosmetic composition for improving acne-containing lacquer extract, it was prepared as shown in Table 7. Here, the lacquer extract is that of Example 8.

TABLE 7

number
Raw material
Formulation Example 4
Comparative Formulation Example 4
One
Sumac extract (Example 8)
10.0
-
2
Beeswax
5.0
5.0
3
Polysorbate 60
1.5
1.5
4
Sorbitan sesquioleate
0.8
0.8
5
Liquid paraffin
40.0
40.0
6
Squalane
5.0
5.0
7
Caprylic / Capric Triglycerides
4.0
4.0
8
Carboxy Vinyl Polymer
0.2
0.2
9
glycerin
5.0
5.0
10
Butylene glycol
3.0
3.0
11
Propylene glycol
3.0
3.0
12
Triethanolamine
0.2
0.2
13
antiseptic
a very small amount
a very small amount
14
Pigment
a very small amount
a very small amount
15
Spices
a very small amount
a very small amount
16
Purified water
Balance
Balance

While mixing 8,9,10,11,13,16 and heating it between 80 ~ 85 ℃ and putting it into the manufacturing part, the emulsifier is activated and 2,3,4,5,6,7,12 is 80 ~ 85 ℃. Heated through and emulsified. After emulsification, the mixture was cooled to 50 ° C. while stirring using a stirrer, and 14 times were added thereto. After cooling to 25 ℃ it was aged.

Formulation Example 5

Formulation Example 5 relates to the nutritional essence of the formulation of the cosmetic composition for improving acne containing lacquer extract, it was prepared as shown in Table 8. Here, the lacquer extract is Example 8

[Table 8]

number
Raw material
Formulation Example 5
Comparative Formulation Example 5
One
Sumac extract (Example 8)
10.0
-
2
Cytostolol
1.70
1.70
3
Polyglyceryl 2-oleate
1.50
1.50
4
Ceramide
0.7
0.7
5
Ceteares-4
1.2
1.2
6
cholesterol
1.5
1.5
7
Dicetylphosphate
0.4
0.4
8
Concentrated glycerin
5.0
5.0
9
Sunflower oil
15.0
15.0
10
Carboxy Vinyl Polymer
0.2
0.2
11
Xanthan Gum
0.2
0.2
12
antiseptic
a very small amount
a very small amount
13
Spices
a very small amount
a very small amount
14
Purified water
Balance
Balance

Non-ionic amphiphilic lipids were prepared by homogenizing the raw materials 2, 3, 4, 5 and 6 at a constant temperature. The nonionic amphiphilic lipid and the raw materials 1,7,8 and 14 are mixed and homogenized at a constant temperature to pass through a microfluidizer, and then the raw material 9 is gradually added at a constant temperature to homogenize and then microfluda again. Passed it through again. 10, 11, 12, 13 was added and dispersed to stabilize and mature.

(2) Skin irritation test method

The experiment on the skin irritation effect of the formulation Examples 1 to 5 was measured as follows.

Ten subjects underwent a skin patch test on the upper arm using a Finn chamber. Psoriasis, eczema, and other skin lesion holders, or those who are pregnant, nursing or contraceptives, or antihistamines, were excluded from this study. Wipe the test site with 70% ethanol and dry it. Then, 10 μl of samples prepared according to Formulation Examples 1 to 5 were added dropwise to a Fin chamber after each sample was prepared in 10 upper arms. It was fixed on the site. After the patch was applied for 24 hours to remove the patch, the test sites were marked with a pen, and after 30 minutes, 24 hours, and 48 hours, the test sites were observed.

The determination was made after 30 minutes of patch removal, after 24 hours, and after 48 hours. Skin response was classified according to the criteria of the International Contact Dermatitis Research Group (ICDRG) in Table 9. As a result of the patch test, skin irritation was determined according to the determination table in Table 10 after calculating the mean score according to the following formula for calculating the average skin reactivity.

[Table 9] Judging Criteria for International Contact Dermatitis

sign
Criteria
Translate
score
-
Negative
Non-irritating
0
± Doubtful or
slight reaction and erytherma Unstimulated (detected as faint or barely
Mild erythema)
0.5
+
Erythema + Induration Hard irritation (a clear but weak erythema,
Edema, papules)
One
++ Erythema + Induration +
Vesicle
Severe stimulation (obvious erythema, papules, vesicles)
2
+++
Erythema + Induration + Bullae
Strong stimulation (severe erythema, alveolar vesicles, skin formation)
3

* Mean score calculation formula

                                   (A + B + C) × 100

 Mean score = ----------------------------------------------- ----

               3 (maximum score) × No. of total subjects × No.of evalution

n n n

A = ∑Score i B = ∑Score j C = ∑Score K

i = 1 j = 1 k = 1

i = subject number 30 minutes after patch removal

j = subject number 24 hours after patch removal

k = subject number 48 hours after patch removal

Score _{i, j, k} = 30 minutes after the patch was removed, 24 hours after the removal, and 48 hours after the evaluation results were applied to both the scores indicated by the score, erythema and edema according to the ICDRG criteria.

TABLE 10

Judgment
Mean score
No irritation (1)
0.00-0.75
Unstimulated (2)
0.76-1.50
Light Stimulation (3)
1.51-2.50
Medium stimulation (4)
2.51-4.00
Strong stimulus (5)
4.01 ~

* Basic Subject Information

Table 11 shows the information of the subjects who participated in this human test.

TABLE 11

No.
name
age
gender
One
SHB
41
M
2
AGN
41
M
3
JWC
40
M
4
YYJ
29
F
5
YMK
35
F
6
KKH
32
M
7
LCI
40
M
8
PWG
29
M
9
PDS
25
M
10
CM
37
F

A total of 10 people participated in this study, consisting of 7 males and 3 females, with an average age of 35 years.

(3) Skin irritation test result

As described above, the results obtained by performing a skin irritation test for each of Formulation Examples 1 to 5 samples are summarized in Tables 12 to 16 below.

TABLE 12 Patch Test Results Table of Formulation Example 1

Table 13 Patch Test Result Table of Formulation Example 2

Table 14 Patch Test Result Table

Table 15 Patch Test Result Table of Formulation Example 4

Table 16: Patch Test Result Table of Formulation Example 5

As can be seen in Tables 12 to 16, Formulation Examples 1 to 5 were all skin irritation test results showed that there is no irritation or unstimulated cosmetic composition.

5. Determination of Acne Improvement Effect of Cosmetic Composition for Improving Acne-Containing Lacquer Extract

Using the prepared Formulation Example 1, the effect on acne dermatitis was tested. To evaluate the improvement effect on acne dermatitis, a clinical trial was conducted on 30 males and females aged 10-30 with acne skin. After classifying acne dermatitis symptoms into three stages according to the degree, the ratio of the degree of acne dermatitis symptom is severe: usually: weak = 1: 1: 1 ratio, and then observe the effect according to each symptom degree. Compared. The evaluation was based on the use of two or more times daily, and after one month of use, the user was evaluated on the following 7-point satisfaction scale. The results are shown in Table 17 below.

TABLE 17

Skin Symptoms Testing personnel Acne improvement Weakness 10 5.3 usually 10 5.7 Severe 10 5.6

According to the results of the evaluation, Formulation Example 1 was found to have an acne-improving effect, and in particular, it was found to be superior to moderate or severe acne skin rather than mild acne skin.

Simple modifications and variations of the present invention can be easily made by those skilled in the art, and all such modifications or changes can be seen to be included in the scope of the present invention.

Claims (7)

In the cosmetic composition for improving acne comprising a lacquer extract, The extract of the lacquer for acne improvement cosmetic composition, characterized in that it comprises 0.5 to 3.0% by weight of camphor roll glycosides. delete The method of claim 1, wherein the lacquer extract is a step of fermenting and ripening raw lacquer for 5 to 30 days, drying the fermented and aged lacquer, and drying the lacquer to dry for 1 to 10 hours to obtain an extract. Acne, cosmetic composition for improving acne characterized in that it is produced by a method comprising the step of aging the extract, filtering the aged extract. The method of claim 1, wherein the lacquer extract is an aqueous solution or water containing 0.01 to 60% by weight of at least one component selected from the group consisting of alcohol, glycerin, alkylene glycol, benzene, chloroform, hexane and acetone as an extractant Acne improvement cosmetic composition, characterized in that to be extracted. The cosmetic composition for improving acne according to claim 1, wherein the lacquer extract is contained in an amount of 0.001 to 30% (w / v) as its dry weight. The cosmetic according to any one of claims 1 to 5, wherein the cosmetic is cosmetics, latexes, creams, packs, powders, foundations, lipsticks, soft liquors, eye cosmetics, soaps, and sunscreens. Acne improvement cosmetic composition, characterized in that any one selected from the group consisting of. Fermenting and maturing the sumac for 5 to 30 days; Drying the fermented and aged lacquer; Steaming the dried lacquer with an extractant for 1 to 10 hours to obtain an extract; Aging the extract; Filtering it; And Mixing the aged extract with an oily raw material; Method for producing a cosmetic composition for improving acne comprising a.