KR101124699B1 - METHOD FOR BREEDING Hosta plantaginea GEUMBAEKRO - Google Patents

Abstract

The present invention relates to a method of growing a daylily new species Geumbaekro, and more particularly, by growing the mutant individual by gamma ray treatment of the daylily cultivar seed, the edge of the leaf gradually grows from pale golden yellow to white and the central part is greenish yellow. The present invention relates to a method for cultivating a daylily new breed Geumbaekro that can cultivate a new daylily with a pattern.

The growing method of daylily new species Geumbaekro according to the present invention, the step of irradiating for 24h at 25Gy dose using ⁶⁰ Co gamma rays as a source of leaf mutation in the daylily cultivars seed without any pattern on the leaves, Obtaining new mutants and asexual breeding of the selected mutant system, wherein the edges of the leaves gradually grow from light golden yellow to white and have a greenish yellow pattern with a greenish yellow pattern. It is characterized by nurturing.

Daylily, Gamma Ray

Description

How to cultivate daylily new breed Geumbaekro {METHOD FOR BREEDING Hosta plantaginea GEUMBAEKRO}

The present invention relates to a method of growing a daylily new species Geumbaekro, and more particularly, by growing the mutant individual by gamma ray treatment of the daylily cultivar seed, the edge of the leaf gradually grows from pale golden yellow to white and the central part is greenish yellow. The present invention relates to a method for cultivating a daylily new breed Geumbaekro that can cultivate a new daylily with a pattern.

Daylily ( Hosta plantaginea ) is a perennial herbaceous plant, 40-56 cm long, with light purple or light white flowers in August-September, oval, 15-22 cm long, 10-17 cm wide, glossy green The part is heart type.

Daylily is used as a plant for differentiation and landscaping, but it is also used as a houseplant or paper plant, but when only the leaves are observed, the color is rather monotonous, and it is too tall to be used for differentiation or flowerbed because it is somewhat monotonous. In order to make up for the shortcomings, it is necessary to cultivate mutant plants with leaves that are short and have high ornamental values.

Most daylily grown as new varieties in Korea depend on the breeding method. As of 2008, three varieties have been registered with the National Seed Management Center. However, in the daylily breeding method, artificial mutation breeding method can play an important role as a niche breeding method that can efficiently breed varieties at low cost in a short period of time.

The important ornamental parts of flower crops are flowers, leaves and grasses, and various breeding methods are applied to foster new varieties of flowers. Among them, artificial mutations using radiation, etc., improve the genetic traits desired by breeders while maintaining the existing good traits. It has the advantage of greatly reducing the breeding period (Cheong et al., 2004; Kwon et al., 1981).

The present invention was conceived by recognizing the above point is an object of the present invention by gamma ray treatment of daylily cultivars seed by asexual propagation of mutated individuals, the edge of the leaf gradually grows from pale golden yellow to white and the central part of green yellow It has the advantage of being able to cultivate a new kind of daylily with a pattern.

In order to achieve the above object, the growing method of daylily new species Geumbaekro according to the present invention is irradiated for 24h at 25Gy dose using gamma ray of ⁶⁰ Co as a source of leaf mutation in the daylily cultivars seed having no pattern on the leaves. And seeding the gamma-ray treated seeds for a plurality of generations to obtain complete mutants having a fixed leaf color or leaf size of the individual among the seeds, and a step of asexual propagation of the selected mutant system by dispensing. Including, daylily new varieties have the following characteristics.

Color: White (N155A)

Makeup: 11cm

Fragrance: Yes

Early (young leaf) edge Leaf color: Yellow (Yellow-group, 3C)

Late leaf color: white group (155B)

Central leaf color: Green group (143C, 144C)

Leaf Length: 17cm

Lobe width: 11.5cm

Lobe type: heart type, slightly wavy edge

According to the above configuration, the method of cultivating daylily new varieties Geumbaekro according to the present invention by gamma-ray treatment of daylily cultivars seed by asexual propagation of the mutated individual, the edge of the leaf gradually grows from pale golden yellow to white and the central part is greenish yellow. It has the advantage of being able to cultivate a new kind of daylily with a pattern.

Hereinafter, with reference to the drawings and examples will be described in more detail the method for growing a daylily new breed Geumbaekro according to the present invention.

The species used to cultivate a new species of Hosta plantaginea (hereinafter referred to as "Geumbaekro") was a cultivar whose green color was both green and growing leaves (Green group, 144A). 1 is a photograph of existing cultivars.

Hosta plantaginea is a perennial herbaceous plant of the family Liliaceae. It is collected and grown in native plants. Its flower length is 40-56cm and the pure white flowers bloom in August-September. Seeds were harvested between October and November 2002, stored at 6 ± 2 ° C in a cold store, and used as samples for gamma ray treatment in March of the following year.

Gamma-ray treatment was performed for 24 hours at the Korea Atomic Energy Research Institute at low levels of 0, 10, 15, 20, 25, and 30 Gy.

Sowing of gamma ray-treated seeds and untreated seeds was carried out on April 17 in Mugaon House of Chonnam Agricultural Research and Development Institute (Wando) by using a connection port containing horticultural soils. It was carried out once every two days to prevent drying, and other management was carried out in accordance with the practice. The germination of M generation ₁ was investigated at the time of germination, germination, germination rate, and survival rate at the interval of 2 ~ 4 days from the end of April, and the selection of the mutant system was different in the color of leaf compared to the mother The leaves were selected on the 25th of October to be divided into individuals with a large difference compared to normal plants. For the selection of the mutant system, on May 25, the plants that were judged to be different from those of the normal plants such as herb and leaf deformation and half-leaf were selected as the mutant system, and the size and number of leaves and leaves were examined on October 20.

The M ₂ V ₁ generation repeated the characterization and selection every year and selected 8 lines from the M ₂ V ₄ generation for characterization and DNA (RAPD) analysis.

After a treatment with gamma rays in daylily seeds M ₁ generation (2003) when using the gamma rays according to the LD ₅₀ represents the closest survival in the 57% survival rate at 30Gy treatment handle the daylily seed is the appropriate treatment dose. In the _second generation of M (2004), 12 dwarf and leaf mutant strains were selected. When gamma rays were irradiated on the daylily seeds as described above, the anti-reflective dose was determined to be 30 Gy, and a total of 12 lines including 3 dwarf mutations, 6 leaf mutations, and 3 variant mutations at the same time in M ₂ generations. Was selected as a promising system.

Table 1 summarizes germination and survival rates of daylily seeds following gamma ray treatment.

Treatment
Dosage (Gy) Sprouting
Time Germination
Time Germination
Rate (%) Seedling survival
Rate (%) 0 May 4 May 9 89 87 10 May 4 May 7 63 61 15 May 4 May 8 63 61 20 May 4 May 10 62 59 25 May 4 May 14 59 57 30 May 4 May 14 57 52

Of the mutation systems selected for the M ₂ V ₁ generation, the variants with fixed variants until the M ₂ V ₄ generation (2007) were 8, 1 in 15Gy, 1 in 20Gy, 2 in 30Gy and 25Gy. Four systems were selected.

FIG. 2 is a photograph of daylily leaves selected from various leaf variants of M ₂ V ₄ generation by gamma ray treatment on daylily seeds.

Figure 3 shows the phylogenetic tree (dendrgram) of the daylily varieties of the selected lineage, referring to Figure 3 actually showed a difference in leaf variation, but the genetic difference by DNA analysis was not large.

HW25-03-01 of the lineage in Figures 2 and 3 is a new breed "Geum Egret" having the characteristics described below.

After 165 days of sowing with gamma-irradiated and untreated seeds, grass height was 4.5cm, while 25Gy treated plants grew 3.5cm. In 2004, four gamma-irradiated 25Gy treatments were used to select four strains in which leaf variations were found in the plant, and in 2005, the strains were transferred to glass greenhouses. This system, which was found to be fixed in the observation process, was preliminarily tested in the glass greenhouse of the Orchard Research Institute (Wando), Jeonnam Agricultural Research and Development Institute, from 2006 to 2008. The new species of daylily is named "Geumbaek-ro", which has the edge of the leaf growing gradually from light golden color to white and the central part has green-yellow pattern.

"Golden egret" is shorter than the existing cultivar and similar in leaf type, but the leaf color is unique compared to the existing species. The front of the leaves of the existing cultivated species is green group (144A), but the "Golden Egret" means that the edges of the leaves grow gradually in the yellow-group (3C) and the white group (155B). Similar to the existing cultivars, the green group (143C, 144C) leaves are 17cm long and 11.5 wide, which is close to the heart and has a weak wavy edge.The leaf surface is glossy and smooth. It is a feeling.

The color is white group (N155A), the same as the cultivated species, and the number of flowers is 8, and the flowers are 11cm long and 8.4cm wide.

The features of the "Gold Egret" as described above are as follows.

1.Color: White (N155A)

2. Makeup: 11cm

3. Fragrance: Yes

4. Color of early (young leaf) edge leaf: Golden (Yellow-group, 3C)

5. Color of leaf at late edge (white leaf): White (155B)

6. The color of the leaf at the center: Green group (143C, 144C)

7.leaf: 17cm

8. Lobe width: 11.5cm

9. Lobe type: heart type, slightly wavy edge

Figures 4a to 4e is a picture showing the daylily new species "gold egret" according to the present invention, Figure 4a is a "gold egret" the whole view, Figure 4b is a view of the young leaves of the leaf edge of the "gold egret" is changed. , FIG. 4C is a view of a late leaf with the leaf edge of "Gold Egret" mutated, FIG. 4D is a bud photo of "Gold Egret", and FIG. 4E is a flower picture of "Gold Egret".

The cultivation of the "Golden Egret" having the above characteristics is managed according to the existing cultivars, and the caution is that the leaves have a pattern, so when cultivated under strong light, the burnt-out phenomena may appear severely. It is good to grow indoors.

Due to the nature of the mutation, depending on the cultivation environment and management methods, the leaf pattern, color and shape of the leaf may be slightly different.

The growth method of the daylily new varieties Geumbaekro described in the above, shown in the drawings is only one embodiment for carrying out the present invention, and should not be construed as limiting the technical idea of the present invention. The scope of protection of the present invention is defined only by the matters set forth in the claims below, and the embodiments improved and changed without departing from the gist of the present invention will be apparent to those skilled in the art. It will be said to belong to the protection scope of the present invention.

1 is a photograph of the existing cultivars

FIG. 2 is a photograph of daylily leaves selected from various leaf mutant systems of M ₂ V ₄ generation by gamma ray treatment on daylily seeds.

FIG. 3 shows a phylogenetic tree (dendrgram) of daylily varieties of selected strains.

4a to 4e are photographs showing the daylily new species "Geumbaekro" according to the present invention;

Claims (1)

Irradiation of daylily cultivars without leaves on leaves for 24h at 25Gy dose using ⁶⁰ Co gamma rays as the source of leaf mutation, and seeding the gamma-rayed seeds for multiple generations, and the color of the individual among the seeds Or obtaining complete mutants having a fixed size of leaves, and augmenting the selected mutant system by dispensing, A growth method of a daylily new breed Geumbaekro to cultivate a daylily new breed having the following characteristics. Color: White (N155A) Makeup: 11cm Fragrance: Yes Early (young leaf) edge Leaf color: Yellow (Yellow-group, 3C) Late leaf color: white group (155B) Central leaf color: Green group (143C, 144C) Leaf Length: 17cm Lobe width: 11.5cm Lobe type: heart type, slightly wavy edge

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