KR101099921B1 - A process for processing lactic acid bacteria proliferator to enhanced flavor - Google Patents

Abstract

The present invention relates to a method for producing a lactic acid bacterium proliferator, and more specifically, after mixing alcoholic extract and purified water, and dissolving it, after undergoing a first deodorization process using aeration and a second deodorization process using activated carbon, By performing the primary filtration step of filtration with a centrifuge and the secondary filtration step of filtration using a filter press, the present invention relates to a method for producing a lactic acid bacterium multiplier, in which odor is removed and the flavor is improved.
Lactic acid bacteria growth method production method according to the present invention,
A raw material mixing step (first step) of mixing the ethanol extract and the purified water;
A stirring step (second step) of dissolving the mixture of the first step;
A first deodorizing step (third step) of deodorizing the solution of the second step using aeration;
A second deodorizing step (fourth step) of deodorizing the first deodorant of the third step using activated carbon;
A first filtration step (fifth step) of filtering the second deodorant liquid of the fourth step with a centrifuge;
A secondary filtration step (sixth step) for filtering the first filtrate of the fifth step using a filter press;
A concentration step (seventh step) of concentrating the second filtrate of the sixth step using a vacuum concentrator; And
And a sterilization step (eighth step) for sterilizing the concentrated liquid of the seventh step.

Description

A process for processing lactic acid bacteria proliferator to enhanced flavor}

The present invention relates to a method for producing lactic acid bacteria growth agent, and more particularly, after mixing alcoholic extract and purified water, followed by a first deodorization process using aeration and a second deodorization process using activated carbon. By performing the primary filtration step of filtration with a centrifuge and the secondary filtration step of filtration using a filter press, the present invention relates to a method for producing a lactic acid bacterium multiplier, in which odor is removed and the flavor is improved.

Modern people are disrupting the balance of gut bacteria due to various factors such as irregular diet, stress, abuse and overuse of antibiotics, fatigue and lack of sleep, and aging. If this balance is broken, the lactic acid bacteria will be reduced, resulting in abnormal metabolism. For example, more harmful bacteria cause problems with fat metabolism, which leads to hyperlipidemia and hypercholesterolemia. In addition, Escherichia coli produces an enzyme that synthesizes 'nitrosoamine', which causes colon cancer, and causes nutritional disorders by breaking down vitamin B1. Among the harmful bacteria, there is a bacterium that synthesizes skin cancer causing factor 'Phenol' and 'Tyramine' which increases blood pressure. In the intestinal environment in which lactic acid bacteria predominate, pathogens that invade the body are not excreted in the intestine but are excreted, but when the balance of intestinal bacteria is broken, the pathogens are easily settled in the intestine and multiply, and eventually, various diseases are caused.

To solve this situation, many studies have been conducted to reduce the proportion of harmful bacteria in our body, and fermented milk products using lactic acid bacteria and lactic acid bacteria effective for maintaining the health of the human body are being actively developed. The role of such lactic acid bacteria is as follows.

First, it boosts the digestive absorption of our food and activates metabolic functions. Fiber is not easily broken down in the intestine, whereas gut bacteria break down the fiber to promote digestion. Second, it is involved in the synthesis of steroid hormones and vitamins B1, B2, folic acid and vitamin K. Third, it is easy to absorb minerals such as iron and calcium, which are difficult to absorb in the intestine. Fourth, to strengthen the immune system to increase the body's resistance. Fifth, the harmful substances or carcinogens are broken down and excreted in vitro through feces. Sixth, intestinal pH is adjusted to the acidic side, and intestinal peristalsis is active by preventing constipation. At the same time, it activates the nutrient absorption of nutrient-absorbing cells in the intestinal wall. Seventh, to prevent the infection of pathogens or harmful bacteria. When intestinal bacteria settle on the wall of the digestive tract, pathogens or harmful bacteria are not located on the barrier, which prevents the growth of harmful bacteria. Eighth, it regulates the digestive absorption of triglycerides and cholesterol ingested through meals, and promotes the excretion of excess triglycerides and cholesterol attached to the inner walls of blood vessels.

Most of the modern people are taking the lactic acid bacteria through commercially available fermented milk, the number of which is not enough to fight the harmful bacteria present in the body.

For this reason, natural food extracts such as unfermented extract, yeast extract, oligosaccharides, carrots, ginger, green onions, etc., made with sake liquor, are used as lactic acid bacteria growth agents to increase the number of lactic acid bacteria and bifidus bacteria in fermented milk products.

However, the use of natural food extracts such as yeast extracts, oligosaccharides, carrots, ginger, green onions, etc. among the lactic acid bacteria growth agents, the growth of lactic acid bacteria and bifidus bacteria have a problem that is still insufficient to combat harmful bacteria present in the body.

In particular, the unfermented extract of the lactic acid bacteria growth agent shows the greatest effect on the growth of lactic acid bacteria and bifidus bacteria, the situation is reluctant to reduce the flavor due to the unique flavor and taste.

As such, lactic acid bacteria and bifidus bacteria are increased without sacrificing flavor and texture, reducing the ratio of harmful bacteria in the body and improving the pleasant effect, it is urgent to develop effective lactic acid bacteria growth agent to maintain the health of the human body.

The present invention has been made to solve the problems of the prior art as described above, an object of the present invention is to mix the ethanol extract and purified water and then stirred, the first deodorization process using aeration and the second using activated carbon After the deodorization process, by performing a primary filtration step of filtration with a centrifuge and a secondary filtration step of filtration using a filter press, the odor is removed already to provide a method for producing lactic acid bacteria growth agent with improved flavor.

Lactic acid bacteria growth method production method according to the present invention for achieving the above object,

A raw material mixing step (first step) of mixing unfermented extract and purified water;

A stirring step (second step) of dissolving the mixture of the first step;

A first deodorizing step (third step) of deodorizing the stirred liquid of the second step using aeration;

A second deodorizing step (fourth step) of deodorizing the first deodorant of the third step using activated carbon;

A first filtration step (fifth step) of filtering the second deodorant liquid of the fourth step with a centrifuge;

A secondary filtration step (sixth step) for filtering the first filtrate of the fifth step using a filter press;

A concentration step (seventh step) of concentrating the second filtrate of the sixth step using a vacuum concentrator; And

And a sterilization step (eighth step) for sterilizing the concentrated liquid of the seventh step.

Hereinafter, described in detail the production method of lactic acid bacteria growth agent of the present invention based on the accompanying drawings.

1 is a process flow diagram schematically showing a method for producing lactic acid bacteria growth agent according to the present invention.

1. Raw material mixing process (1st process)

This process is a process of mixing the raw materials,

By mixing the unfermented extract and purified water, to prepare a mixture so that the concentration of the unfermented extract is 10 to 20% by weight.

The ethanol extract refers to the extract of the by-products remaining after making the sake, if the concentration of the ethanol extract in the mixture is less than 10% by weight can be economical cost due to the water removal of the mixture, if it exceeds 20% by weight There is a difficulty in deodorization is undesirable.

In mixing the raw materials in this process, it is preferable to maintain a temperature of 30 ~ 50 ℃ for ease of mixing, if the temperature is less than 30 ℃ undesired floating and lumps during the raw material mixing is not preferable, 50 ℃ If it exceeds, it is not preferable because the modification of the raw material components may occur.

2. Stirring Process (2nd Process)

This step is a step for dissolving the unfermented extract in purified water in the mixture obtained in the first step,

The mixture of the first step is carried out by stirring at 80 ~ 85 ℃ 10-20 minutes in a stirring tank.

There is no particular limitation on the stirring method in this stirring step, and can be carried out by a conventional method using a conventional stirrer.

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 In stirring the mixture in this process, the stirring time is preferably 10 to 20 minutes, if the time is less than 10 minutes, the dissolution of the mixture is insufficient, may cause suspended matter and lumps, if more than 20 minutes bubbles of the mixture May occur, which is undesirable.

And in order to achieve the ease of stirring a mixture and improving the flavor of a lactic acid bacteria growth agent, it is preferable to keep stirring temperature at 80-85 degreeC.

According to this process, the mixture which passed through the stirring process is called a stirring liquid.

3. First deodorization process (3rd process)

This step is a step of deodorizing the stirred solution first,

The stirring solution of the second step is deodorized for 2 to 3 hours using aeration.

Aeration may be performed by injecting air into the stirring tank. Preferably, the air is filtered from the bottom of the stirring tank by using the air filtered by the ceramic filter and the heaper filter to deodorize the stirring liquid.

Deodorization time in this process is preferably 2 to 3 hours, but if the deodorization time is less than 2 hours, the deodorizing effect of the stirring solution is insufficient, so it is difficult to improve the deodorization of the lactic acid bacteria growth agent is not preferable, if it exceeds 3 hours Is not economical as there is no effect improvement.

In this process, the stirred deodorized liquid is called primary deodorized liquid.

4. Second deodorization process (4th process)

This process is to deodorize the first deodorant again,

Activated charcoal is introduced into the stirring tank with the primary deodorant of the third process to remove the taste, color, odor and organic components contained in the primary deodorant, thereby preparing a secondary deodorant.

The activated carbon is produced by heating the coconut shell at a high temperature, preferably has a particle size of 100 ~ 300mesh, the ability of the methylene blue decolorization to adsorb 180ml (180g of methylene blue 0.12% solution with 1g of activated carbon): Activated charcoal (KS M 1802: Test Method of Activated Carbon) is used to remove and deodorize organic matter in the primary deodorant with specific adsorptive power.

The deodorization of the primary deodorant liquid is to remove as much as possible the taste, color, odor and organic components which are not removed by the primary deodorant.

5. Primary filtration process (5th process)

This process is the process of primary filtration of the second deodorant,

The secondary deodorizing liquid of the fourth process is removed to the maximum by using a centrifugal separator to prepare a primary filtrate.

In order to remove the precipitate as much as possible, this filtration process is preferably carried out using a centrifuge. Specifically, the secondary deodorant of the fourth process is centrifuged at 15,000 rpm or more using a conventional centrifuge, for example, a horizontal continuous centrifuge, and separated into a precipitate having a high specific gravity and a light primary filtrate. do.

6. Second filtration process (sixth process)

This step is to filter the primary filtrate again,

The primary filtrate of the fifth step is filtered under pressure using a filter press so that the concentration of solids in the filtrate is 10 to 20% by weight.

The secondary filtration of the primary filtrate is to remove as much of the precipitate as is not removed by the primary filtration.

If the concentration of the solids in the filtrate is less than 10% by weight, economical costs may occur due to the delay of the working time during the filtration, and if it exceeds 20% by weight, it is not preferable because of the difficulty of filtration.

In the second filtration of the primary filtrate in the present process, the filtration temperature is preferably 60 ~ 90 ℃, if the temperature is less than 60 ℃ may be a slow filtration rate, if it exceeds 90 ℃ filtrate component It is not preferable because denaturation may occur.

And, the filtration pressure is preferably 0 ~ 8kg / ㎠ to remove the precipitate of the filtrate as much as possible.

According to this process, the primary filtrate which passed through the secondary filtration process is called secondary filtrate.

7. Concentration process (7th process)

This process is to concentrate the secondary filtrate,

The secondary filtrate of the sixth step is concentrated to 40 to 60% by weight solids using a vacuum concentrator.

The concentration of the secondary filtrate in the present process so that the solid content is 40 to 60% by weight, for ease of spray drying, if the content of the solid content is less than 40% by weight, economical in accordance with the delay of the operation time during spray drying Cost may occur, and if it exceeds 60% by weight is difficult to spray drying is not preferable.

The secondary filtrate that has undergone the concentration step of this process is called a concentrate.

8. Sterilization Process (8th Process)

This process is to sterilize the concentrate,

The concentrated solution of the seventh step is sterilized for 10-20 minutes at 90 ~ 100 ℃.

At this time, the sterilization temperature is 90 ~ 100 ℃ to prevent the mixing of other microorganisms other than lactic acid bacteria, sterilization time is 20 minutes or less, preferably 10 to 20 in consideration of the efficiency of the process period, while maximizing the sterilization effect It is preferred to be minutes.

According to this process, the concentrate after the sterilization process is called a sterilization solution.

Lactic acid bacteria growth agent prepared according to the present invention can be commercialized by packaging the sterilization liquid in the liquid state prepared as described above.

In addition, the method for producing lactic acid bacteria growth agent of the present invention may further include the following processes after the eighth process, as shown in FIG. 2.

9. Mixing process (ninth process)

This process is a process of blending bactericide and lactose or dextrin,

Lactose or dextrin is blended into the sterilizing solution of the eighth step by adding 10 to 20% by weight relative to the total weight of the final formulation.

The sterilizing solution which has undergone the compounding step of this step is called a blending solution.

10. Spray drying process (Step 10)

This process is a process of spray drying the mixture,

The compounding solution of the ninth step is carried out using a spray dryer.

In this process, a spray dryer is used to spray dissolved substances into heated air or gas to evaporate the water in the concentrate, leaving only the solid powder.

11. Powder filtration process (11th process)

This process is a process of filtering the powder,

The spray-dried powder of the tenth step is filtered to 30 ~ 40mesh using a vibrating body.

The body size of the vibrating body for removing foreign matters in this process is preferably 30 ~ 40mesh, if the body size of the vibrating body is less than 30 mesh is difficult to remove foreign matters, it is not preferable, if more than 40 mesh improves further effects This is uneconomical.

The lactic acid bacteria growth agent prepared according to the present invention may be packaged into a powdered lactic acid bacteria growth agent prepared as described above.

According to the production method of lactic acid bacteria growth agent according to the present invention, by mixing the alcoholic extract and purified water and stirring, by performing a primary deodorization step using aeration and a second deodorization step using activated carbon, unfermented extracts Lactobacillus multiplier with improved aroma and color can be obtained by removing unique smell and color.

In addition, by performing the primary filtration step of filtration with a centrifugal separator and the secondary filtration step of filtration using a filter press, it is possible to obtain a lactic acid bacteria growth agent having improved flavor by removing foreign substances.

1 is a process flow diagram schematically showing a method for producing lactic acid bacteria growth agent according to Example 1 of the present invention.
Figure 2 is a process flow diagram schematically showing a method for producing lactic acid bacteria growth according to Example 2 of the present invention.

The present invention will be described in more detail with reference to the following examples. However, the following examples are only intended to specifically illustrate the present invention, it will be apparent to those skilled in the art that the scope of the present invention is not limited. In other words, simple modifications and variations of the present invention can be easily made by those skilled in the art, and all such modifications and changes are considered to be included within the scope of the present invention.

Example  1: made according to the invention Lactobacillus Growth Agent

The ethanol extract and purified water were mixed at a weight ratio of 1: 5 to prepare a mixture such that the concentration of the ethanol extract is 17% by weight at a temperature of 40 ℃, and dissolved by stirring for 20 minutes at 85 ℃ in a stirring tank. Thereafter, primary deodorization was carried out for 2 hours using aeration, and activated carbon having a particle size of 200 mesh and an MB decolorization force was introduced into a stirring tank having a primary deodorizing solution to prepare a secondary deodorizing solution.

Centrifuge the secondary deodorant at 15,000rpm or more using a horizontal continuous centrifuge, separate the heavy specific precipitate into a light primary filtrate, and filter the primary filtrate with a filter press of 3kg. The secondary filtrate was prepared by pressure filtration to a concentration of 15% by weight at a temperature of / cm 2, 80 ℃.

The secondary filtrate was concentrated using a vacuum concentrator to 50% by weight, and then sterilized at 90 ° C. for 20 minutes to prepare a lactic acid bacteria growth agent.

Example  2 prepared according to the invention Lactobacillus Growth Agent

The ethanol extract and purified water were mixed at a weight ratio of 1: 5 to prepare a mixture such that the concentration of the ethanol extract was 17% by weight at a temperature of 40 ℃, and stirred for 20 minutes at 85 ℃ in a stirring tank. Thereafter, primary deodorization was carried out for 2 hours using aeration, and activated carbon having a particle size of 200 mesh and a methylene blue decolorization force was introduced into a stirring tank having a primary deodorant, thereby preparing a secondary deodorant. .

Centrifuge the secondary deodorant at 15,000rpm or more using a horizontal continuous centrifuge, separate the heavy specific precipitate into a light primary filtrate, and filter the primary filtrate with a filter press of 3kg. The secondary filtrate was prepared by pressure filtration to a concentration of 15% by weight at a temperature of / cm 2, 80 ℃.

The secondary filtrate was concentrated using a vacuum concentrator to 50% by weight, sterilized at 90 ° C. for 20 minutes, and lactose was added by adding 10% by weight to the total weight of the final formulation.

Then, after spraying the blended solution using a spray dryer, the spray-dried powder was filtered through 40mesh to prepare a lactic acid bacteria growth agent.

Comparative example  1: existing Lactobacillus Growth Agent  Manufactured according to the manufacturing method Lactobacillus Growth Agent

A ethanol extract and purified water were mixed at a weight ratio of 1: 5 to prepare a mixture such that the concentration was 17 wt% at a temperature of 40 ° C, and stirred for 20 minutes at 85 ° C in a stirring tank. After centrifugation of the stirred solution at 15,000 rpm or more using a horizontal continuous centrifuge, the precipitate was separated into heavy precipitates and light filtrates, and the filtrate was concentrated to 50% by weight using a vacuum concentrator. , Sterilized for 20 minutes at 90 ℃.

Experiment 1: sensory test

Lactobacillus multiplier prepared according to the present invention (Examples 1 and 2) and Lactobacillus multiplier prepared according to the conventional lactic acid bacteria multiplier (Comparative Example 1) trained professional sensory test personnel (more than 2 years experience sensory test Thirty (50 males and 25 females) with a relatively good taste were selected and divided into five groups (1: very bad, 2: bad, 3) by dividing them into aroma, taste, color, neatness, and preference. : Usually, 4: good, 5: very good). The results are shown in Table 1 below.

incense flavor color neatness preference Example 1 4.87 4.82 4.88 4.81 4.85 Example 2 4.85 4.8 4.89 4.83 4.84 Comparative Example 1 3.45 3.86 4.06 3.79 3.82

As can be seen from Table 1, Examples 1 and 2 show a higher score than Comparative Example 1 in all items of the sensory test.

Examples 1 and 2 showed higher scores in all items than Comparative Example 1 by mixing alcoholic extract and purified water, followed by stirring, followed by a first deodorization step using aeration and a second deodorization step using activated carbon. By removing the peculiar smell and color of alcohol extract, the improved flavor and color of Lactobacillus fungicide was highlighted, and by performing the primary filtration process to filter by centrifuge and the secondary filtration process to filter by filter press. This is because flavor and cleanliness have been improved by removing foreign substances.

Experiment 2: According to Raw Material Bifidobacteria  Measure

In Example 1, except for the difference in the type of raw material, the number of bifidus bacteria of the lactic acid bacteria growth agent (Comparative Examples 2 to 4) prepared in the same manner as in Example 1 was measured, and the results are as follows. Table 2 shows.

division Example 1 Comparative Example 2 Comparative Example 3 Comparative Example 4 Type of raw materials Alcoholic Extract Yeast extract oligosaccharide Carrot Extract Bifidobacteria 100 million ~ 150 million 20 million to 50 million 20-40 million 20-40 million Sensuality
(5-point scale method)
5
2
One
One Judgment fitness incongruity incongruity incongruity

As can be seen from Table 2, the number of bifidus bacteria of Example 1 is significantly higher than that of Comparative Examples 2 to 4. Comparing Example 1 using the ethanol extract and Comparative Examples 2 to 4 using the yeast extract, oligosaccharide, and carrot extract, respectively, Example 1 shows good results in the bifidus bacteria, which is different raw materials from ethanol extract It can be seen that it shows an excellent effect in improving the number of bifidus bacteria compared to.

Claims (8)

A raw material mixing step (first step) of mixing the ethanol extract and the purified water;
A stirring step (second step) of dissolving the mixture of the first step;
A first deodorizing step (third step) of deodorizing the stirred liquid of the second step using aeration;
A second deodorizing step (fourth step) of deodorizing the first deodorant of the third step using activated carbon;
A first filtration step (fifth step) of filtering the second deodorant liquid of the fourth step with a centrifuge;
A secondary filtration step (sixth step) for filtering the first filtrate of the fifth step using a filter press;
A concentration step (seventh step) of concentrating the second filtrate of the sixth step using a vacuum concentrator; And
Method for producing a lactic acid bacteria growth agent comprising a sterilization step (eighth step) for sterilizing the concentrate of the seventh step.
According to claim 1, After the eighth step,
A blending step (ninth step) of blending lactose or dextrin with the sterilizing solution of the eighth step;
A spray drying step (10th step) of spray drying the blended liquid of the ninth step; And
Method for producing a lactic acid bacteria growth agent, characterized in that further comprising a powder filtration step (11th step) to filter the spray-dried powder of the tenth step to 30 ~ 40mesh.
The method according to claim 1 or 2,
In the mixing step of the first step, Lactobacillus multiplier production method characterized in that the ethanol extract and purified water is mixed at a temperature of 30 ~ 50 ℃, so that the concentration of ethanol extract is 10 to 20% by weight.
The method according to claim 1 or 2,
In the stirring step of the second step, the mixture is dissolved by stirring for 10 to 20 minutes at 80 ~ 85 ℃ in a stirring tank for producing a lactic acid bacteria growth agent.
The method according to claim 1 or 2,
In the first deodorization step of the third step, a method for producing lactic acid bacteria growth agent, characterized in that deodorization for 2 to 3 hours using aeration. The method according to claim 1 or 2,
In the second deodorizing step of the fourth step, the lactic acid bacteria growth agent production method characterized in that the use of activated carbon having a particle size of 100 ~ 300mesh, methylene blue decolorization power 180.
The method according to claim 1 or 2,
In the second filtration step of the sixth step, the lactic acid bacteria growth agent production method characterized in that the filtration at a temperature of 60 ~ 90 ℃ so that the concentration of solids in the filtrate is 10 to 20% by weight.
The method of claim 2,
In the blending step of the ninth step, lactobacillus growth agent manufacturing method characterized in that the lactose or dextrin in 10 to 20% by weight relative to the total weight of the final blending compound.

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