KR101092964B1 - New varieties Porphyra tenera of slender-type by pure line method - Google Patents

New varieties Porphyra tenera of slender-type by pure line method Download PDF

Info

Publication number
KR101092964B1
KR101092964B1 KR1020100021674A KR20100021674A KR101092964B1 KR 101092964 B1 KR101092964 B1 KR 101092964B1 KR 1020100021674 A KR1020100021674 A KR 1020100021674A KR 20100021674 A KR20100021674 A KR 20100021674A KR 101092964 B1 KR101092964 B1 KR 101092964B1
Authority
KR
South Korea
Prior art keywords
leaf
weeks
new
varieties
wild type
Prior art date
Application number
KR1020100021674A
Other languages
Korean (ko)
Other versions
KR20110102590A (en
Inventor
황미숙
박은정
황은경
김성철
백재민
공용근
김승오
Original Assignee
대한민국
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 대한민국 filed Critical 대한민국
Priority to KR1020100021674A priority Critical patent/KR101092964B1/en
Publication of KR20110102590A publication Critical patent/KR20110102590A/en
Application granted granted Critical
Publication of KR101092964B1 publication Critical patent/KR101092964B1/en

Links

Images

Classifications

    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01HNEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H13/00Algae
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G33/00Cultivation of seaweed or algae

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Environmental Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Natural Medicines & Medicinal Plants (AREA)
  • Botany (AREA)
  • Developmental Biology & Embryology (AREA)
  • Marine Sciences & Fisheries (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)

Abstract

참김의 새로운 품종 개발을 위하여 진도에서 채집된 참김으로부터 사상체 유도 및 엽체 유도를 통하여 야생형(W)과 신품종(LB)을 선발한 후 유전적 순계를 개발하였다. 두 품종의 특성을 파악하기 위하여 실내배양을 통해 생장과 엽체 형태를 조사하였으며, 이 중 신품종(LB)은 야생형(W)보다 10~20℃에서 엽장 생장이 빠르고 엽체 형태는 세장형을 나타내는 신품종 참김에 관한 것이다.In order to develop new varieties of sesame, wild-type (W) and new varieties (LB) were selected from fowls collected from Jindo through filamentous and leaf-derived induction, and then genetic sequence was developed. In order to understand the characteristics of the two varieties, growth and leaf morphology were examined through indoor culture. Among them, new varieties (LB) showed faster leaf growth at 10-20 ℃ than wild type (W), and the foliar form was elongated. It's about Kim.

Description

순계화 방법에 의한 세장형 신품종 참김{New varieties Porphyra tenera of slender-type by pure line method}New varieties Porphyra tenera of slender-type by pure line method}

본 발명은 참김으로부터 사상체 유도 및 엽체 유도를 통하여 세장형 품종을 선발한 후 유전적 순계 유도방법에 따라 육종 개발된 참김의 신품종에 관한 것이다.The present invention relates to a new breed of sesame seed that has been bred and developed according to the genetic sequencing method after selecting an elongated variety through filamentous induction and leaf body induction from sesame seeds.

김은 분류학상 홍조식물문, 홍조강, 원시홍조아강, 김목, 김과, 김속에 속하는 해조류로 전 세계적으로 현재 133종 알려져 있고, 우리나라에는 17종이 전 연안에 분포하고 있다. 주요 양식종으로는 참김, 방사무늬김, 잇바디돌김, 모무늬돌김 4종이며, 2008년 기준 우리나라 연간 생산량이 22만톤(해조류 생산량의 24.4%), 생산액이 1,940억원(해조류 생산액의 57.7%)으로서 산업적으로 가장 비중 있는 해조류이다.The seaweed belongs to the taxonomy of red algae, red algae, primrose red sea, gimmo, lamiaceae, and gimsok. Currently, 133 seaweeds are known worldwide, and 17 are distributed throughout the coast in Korea. There are four types of aquaculture species: sesame, radiant pattern seaweed, it body seaweed seaweed and hair pattern seaweed seaweed. As of 2008, Korea's annual output is 220,000 tons (24.4% of seaweed production) and 194 billion won (57.7% of seaweed production). It is the most important seaweed in the industry.

이 중 참김과 방사무늬김은 예로부터 양식되어 온 일반 양식김으로서 부드러우며 구멍기가 없으므로 마른김으로 가공된 후 김밥용김과 재래김으로 유통되고 있다. 특히 참김은 70년대 이전까지 우리나라 양식김의 대표 종으로서 맛과 향이 우수하나, 인공채묘가 도입된 이후부터는 환경적응력이 뛰어난 방사무늬김이 주종을 이루게 되었다.Among them, sesame and radish patterned seaweed is a common cultured seaweed that has been cultured since ancient times. Since it is soft and has no holes, it is processed into dried seaweed and is distributed as seaweed laver and traditional seaweed. In particular, sesame seaweed has a good taste and aroma as a representative species of Korean cultured seaweed until the 1970s, but since the introduction of artificial seedlings, radial patterned seaweed with excellent environmental stress has become the dominant species.

일본의 경우도 우리나라와 유사한 상황으로서 인공채묘 도입 후 방사무늬김이 양식장 뿐 아니라 자연개체군에서도 우세하게 됨으로써 결국 참김은 자연 개체군의 유지도 어려워지게 되어 멸종위기종으로 선정되어 관리되어 오고 있는 실정이다.Japan's situation is similar to that of Korea, and after the introduction of artificial seedlings, the radiation pattern laver becomes dominant not only in the farm but also in the natural populations, so it is difficult to maintain the natural populations, and thus, it has been selected and managed as an endangered species.

우리나라에서는 참김이 아직 멸종위기종으로 선정되지는 않았으나 통영, 하동, 청산도 등 소수 지역에서만 생육이 확인되고 있고, 고유의 맛과 향을 가진 종이므로 향후 참김을 이용한 양식종이 개발될 경우 부드러운 촉감과 특유의 향미로 지역특산종으로 개발될 가능성이 크다.Although seaweed is not selected as an endangered species in Korea, its growth is confirmed only in a few regions such as Tongyeong, Hadong, and Cheongsan-do. It is likely to be developed as a local specialty due to its flavor.

우리나라 자생 참김은 엽체 형태가 원형에 가까운 데 비하여 일본에서 수입되고 있는 품종들은 모두 길쭉한 형태의 세장형으로서 방사무늬김에 속한다. 일반적으로 원형 또는 타원형 김은 재래김으로, 세장형 김은 김밥용 김으로 이용되는데, 자생 참김 중에서 세장형 품종이 개발된다면 이는 독특한 촉감과 향미를 지닌 김밥용 김으로 활용될 가능성이 높다.Korean native sesame seed has a leaf form that is close to a circular shape, but all the varieties imported from Japan are elongated, elongated forms and belong to the radiation pattern laver. Generally, round or oval laver is used as traditional laver, and elongated laver is used as laver laver. If elongated varieties are developed among native Korean laver, this is likely to be used as laver laver with unique touch and flavor.

한편 2012년부터는 수산식물(해조류) 분야에도 2012년 UPOV(국제식물신품종보호동맹)에 의한 식물신품종보호제도가 시행될 예정이다. 현재까지 김을 대량으로 생산하고 있는 국가는 한국, 일본, 중국의 3국으로, 그 중 일본에서 다수의 품종이 개발되어 한국과 중국에서 사상체로 유통되고 있다. 따라서 종자전쟁이라고도 불리는 신품종보호제도를 대비하는 차원에서 국산품종 개발을 서둘러야 하며, 다양한 육종기술을 이용하여 신품종을 개발한다면 일본품종에 대한 로열티 지급을 낮추고 나아가 국산품종의 수출을 통하여 로열티 수익을 창출할 수 있을 것이라고 판단된다.In 2012, the new plant protection system by the UPOV (International Plant Protection System for New Plants) will be implemented in the marine plants (seaweed) field. To date, there are three countries producing large amounts of seaweed, including Korea, Japan, and China, among which many varieties have been developed and distributed in Korea and China as Sasang. Therefore, in preparation for the new breed protection system, also called seed war, we must hurry to develop domestic varieties.If we develop new varieties using various breeding techniques, we can lower royalty payments to Japanese varieties and further generate royalties through export of domestic varieties. I think you can.

관련 특허출원으로 국내 등록특허공보 제10-0356102호에는 큰참김과 큰방사무늬김의 종간교잡에 의한 신품종 김 및 그 교잡방법이 개시되어있고, 국내 등록특허공보 제10-0769358호에는 방사무늬김 자생품종의 유전학적 구별방법 및 그에 이용되는 프라이머 세트가 개시되어있다.
As a related patent application, Korean Patent Publication No. 10-0356102 discloses a new breed of laver and its hybridization method by interbreed of large sesame and large radiation pattern laver, and Korean Patent Publication No. 10-0769358 discloses a radiation pattern laver. Genetic differentiation methods of native varieties and primer sets used therein are disclosed.

지구온난화에 대비하고, 어업인 소득을 증대시키기 위해서는 새로운 품종의 김 육종이 시급하다. 일반적으로 원형 또는 타원형 김은 재래김으로, 세장형 김은 김밥용 김으로 이용되므로 원형에 가까운 참김은 주로 재래김으로 가공되어 왔다. 세장형의 참김 신품종을 개발한다면 참김 특유의 부드러운 촉감과 독특한 향미를 지닌 양식김을 생산할 수 있으며, 이를 재래김 뿐 아니라 김밥용 김으로 가공할 수 있을 것이다.New varieties of seaweed breeding are urgently needed to prepare for global warming and to increase the income of fishers. Generally, round or oval laver is used as conventional laver, and elongated laver is used as laver laver. If you develop new varieties of sesame seaweed, you can produce cultured seaweed with soft touch and unique flavor, which can be processed into seaweed laver as well as traditional seaweed.

이와 같은 문제점을 해결하기 위해 참김의 새로운 품종을 개발하고자, 진도에서 채집된 참김으로부터 사상체 유도 및 엽체 유도를 통하여 야생형(W)과 신품종(LB)을 선발한 후 유전적 순계를 개발하였다. 두 품종의 특성을 파악하기 위하여 실내배양을 통해 생장과 엽체 형태를 조사하였으며, 이 중 신품종(LB)은 야생형(W)보다 10~20℃에서 엽장 생장이 빠르고 엽체 형태는 세장형을 나타내는 특징을 보였는바, 이 품종을 새로운 품종으로 제안하고자한다.In order to solve this problem, to develop new varieties of sesame seed, we developed the wild type (W) and new varieties (LB) through filamentous induction and frond induction from sesame seeds collected in Jindo and then developed the genetic sequence. In order to determine the characteristics of the two varieties, growth and leaf morphology were examined through indoor culture. Among them, the new varieties (LB) showed faster leaf growth at 10-20 ℃ than the wild type (W) and the leaf shape showed the elongated form. As you can see, I would like to propose this breed as a new breed.

참김의 새로운 품종 개발을 위하여 진도에서 채집된 참김 계통주로부터 사상체 유도 및 엽체 유도를 통하여 야생형(W)과 세장형 품종(LB)을 선발한 후 유전적 순계를 통한 신품종을 개발하였다.To develop new varieties of sesame seed, wild type (W) and elongate varieties (LB) were selected through filamentous induction and frond induction from sesame seedlings collected in Jindo, and new varieties were developed through genetic sequencing.

우리나라에서는 참김이 아직 멸종위기종으로 선정되지는 않았으나 통영, 하동, 청산도 등 소수 지역에서만 생육이 확인되고 있고, 고유의 맛과 향을 가진 종이므로 향후 참김을 이용한 양식종이 개발될 경우, 부드러운 촉감과 특유의 향미로 지역특산종으로 개발될 가능성이 크다.In Korea, sesame seed has not yet been selected as an endangered species, but growth is only seen in a few regions such as Tongyeong, Hadong, and Cheongsan-do, and has a unique taste and aroma. Due to its unique flavor, it is likely to be developed as a local specialty.

우리나라 자생 참김은 엽체 형태가 원형에 가까운 데 비하여, 일본에서 수입되고 있는 품종들은 모두 길쭉한 형태의 세장형으로서 방사무늬김에 속한다. 일반적으로 원형 또는 타원형 김은 재래김으로, 세장형 김은 김밥용 김으로 이용되는데, 자생 참김 중에서 세장형 품종이 개발된다면 이는 독특한 촉감과 향미를 지닌 양식김을 생산할 수 있으며, 이를 재래김 뿐 아니라 김밥용 김으로 가공할 수 있을 것이다. Korean native sesame seed has a leaf shape that is close to a circular shape, but all the varieties imported from Japan are elongated, elongated forms, and belong to the radiation pattern laver. In general, round or oval laver is used as traditional laver, and elongated laver is used as laver laver. If elongated varieties are developed among native Korean laver, this can produce cultured laver with unique touch and flavor. You can process it with seaweed laver.

또한, 신품종 김은 야생형보다 10~20℃에서 엽장 생장이 빠른 것으로 확인되어 지구온난화에 따른 고수온 현상에도 적합한 품종일 것으로 기대된다.In addition, new varieties of laver are found to grow faster at 10-20 ℃ than wild type, and are expected to be suitable for high water temperature caused by global warming.

한편, 2012년부터는 수산식물(해조류) 분야에도 2012년 UPOV(국제식물신품종보호동맹)에 의한 식물신품종보호제도가 시행될 예정이므로 신품종보호제도를 대비하는 차원에서 국산품종 개발을 서둘러야 하며, 다양한 육종기술을 이용하여 신품종을 개발한다면 일본품종에 대한 로열티 지급을 낮추고 나아가 국산품종의 수출을 통하여 로열티 수익을 창출할 수 있을 것이다.
In 2012, the new plant protection system under the UPOV (International Plant Protection System for New Plants) will be implemented in the marine plants (seaweed) field in 2012. Therefore, it is necessary to hurry to develop domestic breeds in preparation for a new breed protection system. Developing new varieties using technology could lower royalties for Japanese varieties and further generate royalties through exports of domestic varieties.

도 1 김 품종 개발 과정
도 2 유전적 순계 유도 방법
도 3 실내배양 12주 후 야생형(W)의 온도별 엽체 형태
도 4 야생형(W)의 온도별 엽장, 엽폭 및 엽장/엽폭 변화
도 5 실내배양 12주 후 신품종(LB)의 온도별 엽체 형태
도 6 신품종(LB)의 온도별 엽장, 엽폭 및 엽장/엽폭 변화
도 7 신품종(LB)과 야생형(W)의 온도별 엽장 비교
도 8 신품종(LB)과 야생형(W)의 온도별 엽폭 비교
도 9 신품종(LB)과 야생형(W)의 온도별 엽장/엽폭 비교.
Fig. 1 Kim Variety Development Process
Figure 2 Genetic pure system induction method
Figure 3 Leaf shape according to temperature of wild-type (W) after 12 weeks in indoor culture
Figure 4 Leaf, leaf width and leaf / leaf width change according to temperature of wild type (W)
Figure 5 Leaf morphology by temperature of new varieties (LB) after 12 weeks of incubation
Figure 6 Leaf temperature, leaf width and leaf / leaf width change of new varieties (LB)
Figure 7 Comparison of the length of the new species (LB) and wild type (W) by temperature
Figure 8 Leaf width comparison of new varieties (LB) and wild type (W)
Figure 9 Leaf / leaf width comparison of new varieties (LB) and wild type (W).

본 발명은 순계화 방법으로 개발되어 수온이 10~20℃에서 엽장 생장이 빠르고, 엽체 형태를 나타내는 엽장/엽폭은 10~20℃에서 4.66~5.88의 높은 값을 나타내는 세장형을 특징으로 하는 신품종 참김에 관한 것이다.The present invention has been developed by the method of netization, so that the temperature of the leaf growth is fast at 10 ~ 20 ℃, leaf / leaf width showing the morphology of the new species characterized by an elongated type showing a high value of 4.66 ~ 5.88 at 10 ~ 20 ℃ It's about Kim.

또한 본 발명은 엽장의 생장은 12주 배양시 18.00~102.40 mm로 생장하는 것을 신품종 참김을 제공한다.In another aspect, the present invention provides new varieties of sesame seed growth of 18.00 ~ 102.40 mm when grown for 12 weeks.

더욱 자세한 연구개발 방법을 도식화하여 도 1에는 김 품종 개발 과정을, 도 2에는 유전적 순계 유도 방법을 나타내었다.
A more detailed R & D method is shown in FIG. 1 to show the development of laver varieties, and FIG.

1. 유전적 순계 품종개발 및 순계엽체의 유도 및 채묘1. Development of Genetic Cultivated Cultivars and Induction and Harvesting

1) 유전적 순계 개발1) Genetic Strain Development

본 발명에서 사용한 참김(진도) 계통주는 2004년 3월 18일 진도 회동지역 자연개체군에서 채집한 참김 엽체로부터 사상체를 유도하여 국립수산과학원 해조류바이오연구센터에서 배양, 관리해 오던 사상체를 사용하였다.The sesame seed (Jindo) strain used in the present invention used the filament that was incubated and managed in the Seaweed Bio Research Center of the National Fisheries Research and Development Institute by inducing the filamentous body from the sesame seedlings collected from the natural population of Jindo Hoedong area on March 18, 2004.

이 사상체로부터 어린 엽체를 유도하였으며, 어린 엽체 배양 중에 발견된 원형(야생형, W)과 세장형(LB)의 엽체를 선발한 후 순계로서 개발하였다. 순계 개발을 위하여 우선 어린 엽체를 면도칼로 잘게 잘라 수온 조건과 광조건을 15℃, 80μmol*m-2*sec-1, 10L:14D 조건에서 단포자를 유도하였다.The young leaflets were derived from the filaments, and the round (wild type, W) and elongate (LB) leaflets found during the young leaflet culture were selected and developed as a net system. In order to develop the net system, the young leaflets were first chopped with a razor, and the spores were induced under water temperature and light conditions at 15 ° C and 80μmol * m -2 * sec -1 and 10L: 14D.

단포자로부터 발아한 엽체를 분리하여 선발한 후 동일 과정을 3회 반복하여 최종적으로 1개 엽체를 선발하였다. 이 엽체를 자가수정시킨 후 형성된 접합포자를 방출시켜 사상체로 발달하게 하였다. 사상체 중 1개 만을 선발하여 최종적으로 유전적 순계 사상체를 확보하였으며, 이와 같은 유전적 순계 개발은 양 품종의 비교를 위해 야생형(W)과 신품종(LB)에서 각각 수행되었다(도 1). 순계 사상체는 실내배양을 통하여 영구적으로 보존할 수 있으며 필요 시 언제든지 순계 엽체를 생산할 수 있다.
Germinated leaflets were isolated and selected from single spores, and the same procedure was repeated three times to finally select one leaflet. After self fertilization, the leaf spores were released to develop into filamentous filaments. Only one of the filaments was selected to finally secure the hereditary virulence filament. Such genetic virulence development was carried out in wild type (W) and new varieties (LB), respectively, for comparison of both varieties (FIG. 1). Genuine filaments can be permanently preserved through indoor culture and can be produced at any time if necessary.

2) 순계 엽체(배우체) 유도 및 채묘2) Induction and Seedling

순계 사상체를 20℃, 30μmol*m-2*sec-1, 14L:10D(장일) 조건에서 배양한 후 10L:14L(단일) 조건으로 변화시켜 각포자낭을 형성시켰다. 이 각포자낭이 충분히 성숙하였을 때 온도를 15℃로 내려주어 각포자 방출을 유도하였고, 배양용기에 나일론 섬유를 넣어 방출된 각포자를부착(채묘)시킨 후 새로운 엽체의 발달을 유도하였다(도 2).
Pure filaments were incubated at 20 ° C., 30 μmol * m −2 * sec −1 , 14L: 10D (long day), and then changed to 10L: 14L (single) condition to form keratocytes. When the cornea was sufficiently matured, the temperature was lowered to 15 ° C. to induce release of the cornea, and the development of new leaflets was induced after attaching (seeding) the released corneal spores into the culture vessel (Fig. 2). ).

2. 품종 개발된 참김과 야생형 참김의 특성비교2. Comparison of Characteristics of Varieties of Developing Sesame and Wild-type Sesame

1) 실내배양에 의한 생장 및 엽체 형태 조사1) Growth and Leaf Form Examination by Indoor Culture

야생형(W)과 신품종(LB)의 유전적 순계 엽체의 특성을 조사하기 위하여 온도(5, 10, 15, 20℃), 광도(40μmol*m-2*sec-1), 광주기(10L:14D) 조건에서 12주 동안 배양하면서 1주 간격으로 온도별 엽장과 엽폭 생장을 조사하였다. 또한 엽체의 형태를 확인하기 위하여 엽장/엽폭을 조사하였다.
Temperature (5, 10, 15, 20 ° C), luminous intensity (40μmol * m -2 * sec -1 ), photoperiod (10L :) to investigate the properties of genetically pure leaves of wild type (W) and new varieties (LB) 14D) were examined for the temperature and leaf width growth by temperature at weekly intervals for 12 weeks under conditions. The leaf / leaf width was also examined to determine the shape of the leaf.

2) 야생형(W)의 생장 및 엽체 형태 2) Growth and Leaf Forms of Wild-type (W)

도 3은 실내배양 12주 후 야생형(W)의 온도별 엽체 형태를 나타낸다. 표 1 - 표 3에는 야생형의 온도별 엽장, 엽폭 및 엽장/엽폭 변화를 수치적으로 나타내었으며, 도 4에는 야생형의 온도별 엽장, 엽폭 및 엽장/엽폭 변화를 도식화하여 나타내었다.Figure 3 shows the morphology of the morphology of the wild-type (W) temperature after 12 weeks of incubation. Table 1-Table 3 shows the changes in the wild type temperature, leaf width and leaf / leaf width by temperature, and Figure 4 shows the changes in the wild type temperature, leaf width and leaf / leaf width.

채묘 후 12주 동안 엽장을 조사한 결과, 5℃에서는 12주 째 91 mm로서 가장 높았으며 10℃에서 77 mm, 15℃에서 38 mm, 그리고 20℃에서 10 mm로서 온도가 높아질수록 엽장이 낮아졌다. 12주 째 엽폭은 15℃에서 29 mm로서 가장 높았으며 10℃에서 26 mm, 5℃에서 13 mm, 20℃에서는 13 mm 순이었으며, 엽장/엽폭은 5℃에서 6.8로서 가장 높았고 10, 15, 20℃에서 각각 2.9, 1.3, 0.8로서, 온도가 높아질수록 세장형에서 점차 원형으로 변하였다.
After 12 weeks of harvesting, the leaves were the highest at 91 ° C at 5 ° C and 77 mm at 10 ° C, 38 mm at 15 ° C, and 10 mm at 20 ° C. At 12 weeks, the leaf width was the highest at 29 ° C (29 mm), followed by 26 mm at 10 ° C, 13 mm at 5 ° C, and 13 mm at 20 ° C. The leaf / leaf width was the highest at 6.8 ° C at 5 ° C (10, 15, 20). It was 2.9, 1.3, and 0.8 at ° C, respectively, and gradually changed from elongate to circular with increasing temperature.

야생형(W)의 엽장 변화 (단위: mm)Changes in the length of wild type (W) in mm 0주Week 0 1주1 week 2주2 weeks 3주3 weeks 4주4 Weeks 5주5 Weeks 6주6 weeks 7주Week 7 8주8 Weeks 9주9 Weeks 10주10 Weeks 11주Week 11 12주12 Weeks 5℃5 ℃ 평균Average 0.05 0.05 0.15 0.15 0.19 0.19 0.24 0.24 0.40 0.40 0.72 0.72 3.20 3.20 6.40 6.40 14.2714.27 28.17 28.17 44.70 44.70 63.20 63.20 91.42 91.42 표준편차Standard Deviation 0.01 0.01 0.02 0.02 0.03 0.03 0.15 0.15 0.21 0.21 0.33 0.33 0.80 0.80 1.66 1.66 3.38 3.38 7.30 7.30 10.83 10.83 14.04 14.04 22.25 22.25 10℃10 ℃ 평균Average 0.05 0.05 0.12 0.12 0.30 0.30 0.52 0.52 0.94 0.94 1.70 1.70 2.96 2.96 5.65 5.65 11.76 11.76 25.52 25.52 41.06 41.06 58.63 58.63 77.08 77.08 표준편차Standard Deviation 0.01 0.01 0.02 0.02 0.06 0.06 0.12 0.12 0.29 0.29 0.57 0.57 1.61 1.61 2.42 2.42 5.91 5.91 13.65 13.65 21.51 21.51 30.84 30.84 41.53 41.53 15℃15 ℃ 평균Average 0.05 0.05 0.16 0.16 0.27 0.27 0.50 0.50 0.71 0.71 1.17 1.17 2.09 2.09 4.90 4.90 7.88 7.88 13.75 13.75 21.48 21.48 29.51 29.51 37.58 37.58 표준편차Standard Deviation 0.01 0.01 0.03 0.03 0.06 0.06 0.14 0.14 0.28 0.28 0.54 0.54 1.21 1.21 1.69 1.69 2.86 2.86 6.94 6.94 11.79 11.79 17.45 17.45 22.23 22.23 20℃20 ℃ 평균Average 0.05 0.05 0.23 0.23 0.31 0.31 0.62 0.62 0.88 0.88 1.25 1.25 1.70 1.70 2.01 2.01 2.68 2.68 4.43 4.43 6.55 6.55 8.14 8.14 9.82 9.82 표준편차Standard Deviation 0.01 0.01 0.02 0.02 0.06 0.06 0.19 0.19 0.20 0.20 0.28 0.28 0.22 0.22 0.32 0.32 0.57 0.57 1.08 1.08 1.83 1.83 2.82 2.82 3.43 3.43

야생형(W)의 엽폭 변화 (단위: mm)Leaf width change of wild type (W) in mm 0주Week 0 1주1 week 2주2 weeks 3주3 weeks 4주4 Weeks 5주5 Weeks 6주6 weeks 7주Week 7 8주8 Weeks 9주9 Weeks 10주10 Weeks 11주Week 11 12주12 Weeks 5℃5 ℃ 평균Average 0.01 0.01 0.04 0.04 0.07 0.07 0.09 0.09 0.14 0.14 0.28 0.28 1.39 1.39 2.05 2.05 3.68 3.68 5.81 5.81 8.01 8.01 10.23 10.23 13.40 13.40 표준편차Standard Deviation 0.00 0.00 0.01 0.01 0.01 0.01 0.05 0.05 0.05 0.05 0.13 0.13 0.40 0.40 0.30 0.30 0.44 0.44 0.72 0.72 1.01 1.01 1.33 1.33 1.72 1.72 10℃10 ℃ 평균Average 0.01 0.01 0.03 0.03 0.10 0.10 0.18 0.18 0.45 0.45 0.85 0.85 1.48 1.48 2.73 2.73 5.58 5.58 10.22 10.22 15.94 15.94 21.54 21.54 26.23 26.23 표준편차Standard Deviation 0.00 0.00 0.01 0.01 0.01 0.01 0.07 0.07 0.14 0.14 0.42 0.42 0.79 0.79 0.98 0.98 2.01 2.01 3.49 3.49 5.33 5.33 6.89 6.89 7.68 7.68 15℃15 ℃ 평균Average 0.01 0.01 0.05 0.05 0.08 0.08 0.24 0.24 0.41 0.41 0.81 0.81 1.54 1.54 3.61 3.61 6.44 6.44 10.40 10.40 15.93 15.93 20.84 20.84 28.93 28.93 표준편차Standard Deviation 0.00 0.00 0.01 0.01 0.02 0.02 0.08 0.08 0.15 0.15 0.35 0.35 0.78 0.78 0.94 0.94 2.23 2.23 3.62 3.62 6.10 6.10 7.55 7.55 11.53 11.53 20℃20 ℃ 평균Average 0.01 0.01 0.05 0.05 0.08 0.08 0.15 0.15 0.46 0.46 0.83 0.83 1.46 1.46 2.17 2.17 3.29 3.29 5.38 5.38 8.08 8.08 10.81 10.81 12.64 12.64 표준편차Standard Deviation 0.00 0.00 0.01 0.01 0.01 0.01 0.04 0.04 0.09 0.09 0.24 0.24 0.25 0.25 0.54 0.54 0.98 0.98 1.80 1.80 2.71 2.71 3.53 3.53 4.65 4.65

야생형(W)의 엽장/엽폭 변화 Leaf / leaf width change of wild type (W) 0주Week 0 1주1 week 2주2 weeks 3주3 weeks 4주4 Weeks 5주5 Weeks 6주6 weeks 7주Week 7 8주8 Weeks 9주9 Weeks 10주10 Weeks 11주Week 11 12주12 Weeks 5℃5 ℃ 평균Average 3.82 3.82 3.70 3.70 2.90 2.90 2.87 2.87 2.90 2.90 2.60 2.60 2.30 2.30 3.12 3.12 3.87 3.87 4.85 4.85 5.58 5.58 6.18 6.18 6.82 6.82 표준편차Standard Deviation 3.21 3.21 2.77 2.77 2.98 2.98 3.19 3.19 4.17 4.17 2.55 2.55 2.01 2.01 5.49 5.49 7.75 7.75 10.16 10.16 10.70 10.70 10.53 10.53 12.93 12.93 10℃10 ℃ 평균Average 3.82 3.82 4.71 4.71 2.96 2.96 2.83 2.83 2.11 2.11 2.01 2.01 2.00 2.00 2.07 2.07 2.11 2.11 2.50 2.50 2.58 2.58 2.72 2.72 2.94 2.94 표준편차Standard Deviation 3.21 3.21 3.24 3.24 4.65 4.65 1.80 1.80 2.05 2.05 1.38 1.38 2.04 2.04 2.47 2.47 2.94 2.94 3.91 3.91 4.03 4.03 4.47 4.47 5.41 5.41 15℃15 ℃ 평균Average 3.82 3.82 3.58 3.58 3.13 3.13 2.11 2.11 1.71 1.71 1.44 1.44 1.35 1.35 1.36 1.36 1.22 1.22 1.32 1.32 1.35 1.35 1.42 1.42 1.30 1.30 표준편차Standard Deviation 3.21 3.21 3.89 3.89 2.39 2.39 1.74 1.74 1.90 1.90 1.53 1.53 1.55 1.55 1.80 1.80 1.28 1.28 1.92 1.92 1.93 1.93 2.31 2.31 1.93 1.93 20℃20 ℃ 평균Average 3.82 3.82 4.77 4.77 3.71 3.71 4.11 4.11 1.92 1.92 1.50 1.50 1.17 1.17 0.93 0.93 0.82 0.82 0.82 0.82 0.81 0.81 0.75 0.75 0.78 0.78 표준편차Standard Deviation 3.21 3.21 3.76 3.76 4.34 4.34 4.80 4.80 2.27 2.27 1.17 1.17 0.91 0.91 0.59 0.59 0.58 0.58 0.60 0.60 0.68 0.68 0.80 0.80 0.74 0.74

3) 신품종(LB)의 생장 및 엽체 형태 3) Growth and Leaf Forms of New Varieties

도 5는 실내배양 12주 후 신품종(LB)의 온도별 엽체 형태를 나타낸다. 표 4 - 표 6에는 신품종의 온도별 엽장, 엽폭 및 엽장/엽폭 변화를 수치적으로 나타내었으며, 도 6에는 신품종의 온도별 엽장, 엽폭 및 엽장/엽폭 변화를 도식화하여 나타내었다.Figure 5 shows the morphology of the temperature of the new varieties (LB) after 12 weeks of incubation. Table 4-Table 6 shows the numerical changes of leaf length, leaf width, and leaf / leaf width according to the temperature of new varieties, and FIG. 6 shows the change of the leaf length, leaf width, and leaf / leaf width according to temperature of new varieties.

채묘 후 12주 동안 엽장을 조사한 결과, 8주까지는 10, 15, 5, 20℃ 순이었으나 그 이후에는 15, 10, 5, 20℃ 순이었으며, 5, 10, 15℃에서는 12주 째 100 mm 가까이 엽장이 높아졌으나 20℃에서는 18 mm에 불과하였다. 12주 째 엽폭은 15℃에서 22 mm로서 가장 높았으며 5, 10℃는 17 mm로 유사하였고 20℃에서는 13 mm로 가장 낮았다. 엽장/엽폭은 10℃에서 가장 높았으며 5, 15, 20℃ 순이었음. 12주 째 5, 10, 15℃에서는 각각 5.71, 5.88, 4.66의 값으로서 세장형이었으나 20℃에서는 1.36으로서 원형에 가까웠다.
The leaves were examined for 12 weeks after seeding, followed by 10, 15, 5, and 20 ℃ for up to 8 weeks, but after that, 15, 10, 5, and 20 ℃ were used, and after 5 weeks, near 100 mm for 12 weeks. The lobe increased but was only 18 mm at 20 ℃. At 12 weeks, the leaf width was the highest at 15 ° C (22 mm) and similar at 5 and 10 ° C (17 mm) and the lowest at 20 ° C (13 mm). The leaf / leaf width was the highest at 10 ° C, followed by 5, 15, and 20 ° C. At 5th, 10th, and 15th degree C at the 12th week, they were elongate as 5.71, 5.88, and 4.66, respectively.

신품종(LB)의 엽장 변화 (단위: mm)Changes in Leaf Length of New Varieties (LB) in mm 0주Week 0 1주1 week 2주2 weeks 3주3 weeks 4주4 Weeks 5주5 Weeks 6주6 weeks 7주Week 7 8주8 Weeks 9주9 Weeks 10주10 Weeks 11주Week 11 12주12 Weeks 5℃5 ℃ 평균Average 0.08 0.08 0.13 0.13 0.27 0.27 0.34 0.34 0.45 0.45 1.87 1.87 3.77 3.77 7.16 7.16 15.85 15.85 28.97 28.97 46.74 46.74 72.23 72.23 98.31 98.31 표준편차Standard Deviation 0.01 0.01 0.02 0.02 0.03 0.03 0.18 0.18 0.18 0.18 0.19 0.19 0.69 0.69 1.04 1.04 2.54 2.54 4.65 4.65 7.42 7.42 11.85 11.85 16.35 16.35 10℃10 ℃ 평균Average 0.08 0.08 0.15 0.15 0.25 0.25 0.99 0.99 2.64 2.64 6.04 6.04 14.12 14.12 28.01 28.01 36.26 36.26 46.44 46.44 59.67 59.67 79.74 79.74 100.42 100.42 표준편차Standard Deviation 0.01 0.01 0.02 0.02 0.10 0.10 0.15 0.15 0.52 0.52 1.31 1.31 2.06 2.06 5.66 5.66 6.18 6.18 9.08 9.08 10.84 10.84 13.79 13.79 17.59 17.59 15℃15 ℃ 평균Average 0.08 0.08 0.18 0.18 0.80 0.80 0.89 0.89 1.49 1.49 3.10 3.10 7.38 7.38 17.51 17.51 37.07 37.07 55.56 55.56 67.61 67.61 83.16 83.16 104.44 104.44 표준편차Standard Deviation 0.01 0.01 0.03 0.03 0.25 0.25 0.17 0.17 0.29 0.29 0.62 0.62 1.32 1.32 2.93 2.93 6.01 6.01 8.62 8.62 10.62 10.62 12.86 12.86 14.55 14.55 20℃20 ℃ 평균Average 0.08 0.08 0.21 0.21 0.29 0.29 0.40 0.40 0.69 0.69 1.16 1.16 1.81 1.81 3.00 3.00 4.99 4.99 8.95 8.95 11.58 11.58 15.57 15.57 17.95 17.95 표준편차Standard Deviation 0.01 0.01 0.03 0.03 0.05 0.05 0.09 0.09 0.09 0.09 0.18 0.18 0.30 0.30 0.65 0.65 1.61 1.61 3.36 3.36 4.43 4.43 5.79 5.79 6.04 6.04

신품종(LB)의 엽폭 변화 (단위: mm)Leaf width change of new varieties (LB) in mm 0주Week 0 1주1 week 2주2 weeks 3주3 weeks 4주4 Weeks 5주5 Weeks 6주6 weeks 7주Week 7 8주8 Weeks 9주9 Weeks 10주10 Weeks 11주Week 11 12주12 Weeks 5℃5 ℃ 평균Average 0.02 0.02 0.05 0.05 0.11 0.11 0.15 0.15 0.20 0.20 0.77 0.77 1.62 1.62 2.55 2.55 4.70 4.70 6.93 6.93 10.09 10.09 13.76 13.76 17.22 17.22 표준편차Standard Deviation 0.00 0.00 0.01 0.01 0.02 0.02 0.07 0.07 0.09 0.09 0.08 0.08 0.24 0.24 0.29 0.29 0.55 0.55 0.89 0.89 1.12 1.12 1.72 1.72 2.20 2.20 10℃10 ℃ 평균Average 0.02 0.02 0.06 0.06 0.09 0.09 0.40 0.40 1.20 1.20 2.11 2.11 3.12 3.12 5.48 5.48 6.61 6.61 8.13 8.13 10.42 10.42 13.93 13.93 17.09 17.09 표준편차Standard Deviation 0.00 0.00 0.01 0.01 0.04 0.04 0.05 0.05 0.18 0.18 0.40 0.40 0.54 0.54 0.86 0.86 1.19 1.19 1.43 1.43 1.64 1.64 2.14 2.14 2.78 2.78 15℃15 ℃ 평균Average 0.02 0.02 0.07 0.07 0.37 0.37 0.36 0.36 0.72 0.72 1.78 1.78 3.02 3.02 5.86 5.86 9.57 9.57 13.17 13.17 15.50 15.50 19.19 19.19 22.40 22.40 표준편차Standard Deviation 0.00 0.00 0.01 0.01 0.09 0.09 0.06 0.06 0.13 0.13 0.22 0.22 0.41 0.41 0.95 0.95 1.53 1.53 2.90 2.90 2.57 2.57 3.64 3.64 3.64 3.64 20℃20 ℃ 평균Average 0.02 0.02 0.07 0.07 0.12 0.12 0.20 0.20 0.38 0.38 0.85 0.85 1.44 1.44 2.88 2.88 4.27 4.27 6.74 6.74 8.95 8.95 11.36 11.36 13.23 13.23 표준편차Standard Deviation 0.00 0.00 0.01 0.01 0.02 0.02 0.02 0.02 0.08 0.08 0.22 0.22 0.37 0.37 0.61 0.61 0.94 0.94 1.91 1.91 2.36 2.36 3.24 3.24 3.52 3.52

신품종(LB)의 엽장/엽폭 변화Leaf / leaf width changes of new varieties (LB) 0주Week 0 1주1 week 2주2 weeks 3주3 weeks 4주4 Weeks 5주5 Weeks 6주6 weeks 7주Week 7 8주8 Weeks 9주9 Weeks 10주10 Weeks 11주Week 11 12주12 Weeks 5℃5 ℃ 평균Average 3.59 3.59 2.61 2.61 2.47 2.47 2.27 2.27 2.21 2.21 2.42 2.42 2.33 2.33 2.80 2.80 3.37 3.37 4.18 4.18 4.63 4.63 5.25 5.25 5.71 5.71 표준편차Standard Deviation 0.64 0.64 0.31 0.31 0.27 0.27 0.23 0.23 0.27 0.27 0.23 0.23 0.52 0.52 0.34 0.34 0.50 0.50 0.70 0.70 0.78 0.78 1.08 1.08 1.11 1.11 10℃10 ℃ 평균Average 3.59 3.59 2.67 2.67 2.72 2.72 2.49 2.49 2.20 2.20 2.86 2.86 4.53 4.53 5.11 5.11 5.49 5.49 5.71 5.71 5.73 5.73 5.72 5.72 5.88 5.88 표준편차Standard Deviation 0.64 0.64 0.34 0.34 0.45 0.45 0.39 0.39 0.52 0.52 0.70 0.70 0.74 0.74 0.82 0.82 1.01 1.01 1.06 1.06 1.23 1.23 1.35 1.35 1.36 1.36 15℃15 ℃ 평균Average 3.59 3.59 2.63 2.63 2.15 2.15 2.46 2.46 2.07 2.07 1.74 1.74 2.44 2.44 2.99 2.99 3.87 3.87 4.22 4.22 4.36 4.36 4.33 4.33 4.66 4.66 표준편차Standard Deviation 0.64 0.64 0.44 0.44 0.47 0.47 0.62 0.62 0.55 0.55 0.34 0.34 0.60 0.60 0.74 0.74 0.96 0.96 1.02 1.02 0.98 0.98 1.07 1.07 0.99 0.99 20℃20 ℃ 평균Average 3.59 3.59 3.08 3.08 2.43 2.43 2.01 2.01 1.81 1.81 1.37 1.37 1.26 1.26 1.04 1.04 1.17 1.17 1.31 1.31 1.29 1.29 1.37 1.37 1.36 1.36 표준편차Standard Deviation 0.64 0.64 0.55 0.55 0.61 0.61 0.56 0.56 0.43 0.43 0.31 0.31 0.33 0.33 0.25 0.25 0.38 0.38 0.45 0.45 0.45 0.45 0.50 0.50 0.35 0.35

4) 신품종(LB)과 야생형(W)의 생장 및 엽체 형태 비교4) Growth and Leaf Morphology of New Varieties (LB) and Wild-type (W)

신품종(LB)과 야생형(W)의 생장 및 엽체 형태를 비교하면 다음과 같다. 도 7은 신품종(LB)과 야생형(W)의 온도별 엽장을 비교한 결과를 나타내며, 도 8은 신품종(LB)과 야생형(W)의 온도별 엽폭의 변화를 나타내며, 도 9는 신품종(LB)과 야생형(W)의 온도별 엽장/엽폭의 비교를 나타낸다.
Comparing the growth and leaf shape of the new breed (LB) and wild type (W) is as follows. Figure 7 shows the result of comparing the temperature of the new species (LB) and wild type (W) by the temperature, Figure 8 shows the change of the leaf width by temperature of the new type (LB) and wild type (W), Figure 9 is a new breed (LB) ) Shows the comparison of the leaf / leaf width by temperature of wild type (W).

가) 수온에 따른 엽장을 비교하면 다음과 같다. A) Comparing the leaf length according to the water temperature is as follows.

5℃로 12주 동안 배양하였을 때 신품종(LB)은 98.3 mm, 야생형(W)은 91.4 mm로서 신품종(LB)의 엽장생장이 조금 더 빨랐고, 10℃로 12주 동안 배양하였을 때 신품종(LB)은 100.4 mm, 야생형(W)은 77.1 mm로서 신품종(LB)의 엽장생장이 1.3배 더 빨랐다. When it was incubated at 5 ℃ for 12 weeks, the new species (LB) was 98.3 mm and wild type (W) was 91.4 mm, and the growth rate of the new species (LB) was slightly faster. 100.4 mm of silver and 77.1 mm of wild type (W) showed 1.3 times faster leaf growth of new varieties (LB).

15℃로 12주 동안 배양하였을 때 신품종(LB)은 104.4 mm, 야생형(W)은 37.6 mm로서 신품종(LB)의 엽장생장이 2.8배 더 빨랐으며, 20℃로 12주 동안 배양하였을 때 신품종(LB)은 18.0 mm, 야생형(W)은 9.8 mm로서 신품종(LB)의 엽장생장이 1.8배 더 빨랐다. 이와 같은 결과를 도 7에 신품종(LB)과 야생형(W)의 온도별 엽장을 비교한 결과로 나타내었다.
When cultured at 15 ° C for 12 weeks, the new species (LB) was 104.4 mm and wild type (W) was 37.6 mm, which showed 2.8 times faster leaf growth of new varieties (LB). LB) was 18.0 mm and wild type (W) was 9.8 mm, which showed 1.8 times faster leaf growth of new varieties (LB). Such results are shown in FIG. 7 as a result of comparing the temperature of the new species (LB) and wild type (W) according to the temperature.

나) 수온에 따른 엽폭을 비교하면 다음과 같다. B) Comparison of leaf width according to water temperature is as follows.

5℃로 12주 동안 배양하였을 때 신품종(LB)은 17.2 mm, 야생형(W)은 13.4 mm로서 신품종(LB)의 엽폭 생장이 1.3배 더 빨랐고, 10℃로 12주 동안 배양하였을 때 신품종(LB)은 17.1 mm, 야생형(W)은 26.2 mm로서 야생형(W)의 엽폭 생장이 1.5배 더 빨랐다.When it was incubated at 5 ℃ for 12 weeks, the new species (LB) was 17.2 mm, wild type (W) was 13.4 mm, and the leaf growth of the new species (LB) was 1.3 times faster, and when it was incubated at 10 ℃ for 12 weeks, ) Showed 17.1 mm, wild type (W) was 26.2 mm, and the leaf growth of wild type (W) was 1.5 times faster.

15℃로 12주 동안 배양하였을 때 신품종(LB)은 22.4 mm, 야생형(W)은 28.9 mm로서 야생형(W)의 엽폭 생장이 1.3배 더 빨랐고, 20℃로 12주 동안 배양하였을 때 신품종(LB)은 13.2 mm, 야생형(W)은 12.6 mm로서 신품종(LB)의 엽장 생장이 조금 더 빨랐다. 이와 같은 결과를 정리하여 도 8에 신품종(LB)과 야생형(W)의 온도별 엽폭의 변화를 비교하여 나타내었다.
When it was incubated for 12 weeks at 15 ° C, the new species (LB) was 22.4 mm, wild type (W) was 28.9 mm, and the leaf growth of wild type (W) was 1.3 times faster, and when it was incubated for 12 weeks at 20 ° C, ), The wild type (W) was 12.6 mm, the growth of the new species (LB) was slightly faster. These results are summarized and shown in Figure 8 by comparing the change in the leaf width of the new species (LB) and wild type (W) by temperature.

다) 수온에 따른 엽체 형태(엽장/엽폭)를 비교하면 다음과 같다.C) Leaf shape (leaf / leaf width) according to water temperature is as follows.

5℃에서는 채묘직후 3.6에서 시작하여 6주 이후부터 다시 높아져 12주 째 신품종(LB)은 5.7, 야생형(W)은 6.8로서 야생형(W)의 값이 조금 더 높았다. 10℃에서는 신품종(LB)은 4주 이후 높아져 12주 째 5.8이었고, 야생형(W)은 8주 이후 조금씩 높아져 12주 째 2.9 였으므로, 신품종(LB)이 야생형(W)보다 2배 정도 길쭉한 형태를 나타내었다.At 5 ° C, it started at 3.6 after harvesting and increased again after 6 weeks. At 12 weeks, the new breed (LB) was 5.7 and wild type (W) was 6.8, which was slightly higher. At 10 ℃, the new breed (LB) increased after 4 weeks and was 5.8 at 12 weeks, while the wild type (W) was slightly higher after 8 weeks and was 2.9 at 12 weeks, so the new breed (LB) was twice as long as the wild type (W). Indicated.

15℃에서는 신품종(LB)은 5주 이후 높아져 12주 째 4.7이었고, 야생형(W)은 채묘이후 지속적으로 낮아져 12주 째 1.3이었으므로, 신품종(LB)이 야생형(W)보다 3.6배 정도 길쭉한 형태를 나타내었다. 20℃에서는 신품종(LB)과 야생형(W) 모두 채묘 후 지속적으로 값이 낮아져 12주에는 신품종(LB) 1.4, 야생형(W) 0.8로서 1.8배 정도 길쭉한 형태를 나타내었다. 이와 같은 결과를 정리하여 도 9에 신품종(LB)과 야생형(W)의 온도별 엽장/엽폭의 비교 결과로 정리하였다.
At 15 ℃, the new breed (LB) increased after 5 weeks and was 4.7 at 12 weeks, while the wild type (W) was continuously lowered after harvesting and was 1.3 at 12 weeks, so the new breed (LB) was 3.6 times longer than the wild type (W). Indicated. At 20 ° C, both new varieties (LB) and wild type (W) were continuously lowered after harvesting, and at 12 weeks, the new varieties (LB) and wild type (W) 0.8 were 1.8 times longer. These results are summarized in Fig. 9 as a comparison result of the leaf length / leaf width according to the temperature of the new breed (LB) and wild type (W).

3. 신품종김과 야생형(W)의 생장 차이점3. Growth Differences Between New Breed Kim and Wild-type (W)

1) 배양에 따른 형태의 차이점1) Differences in form by culture

참김은 일반적으로 채묘시기인 23~24℃를 지나면서 온도가 낮아지는 조건에 적응하며 생장하며. 생장 온도가 20℃ 이상이면 단포자 방출이 활발하여 엽장 생장이 떨어지게 되며, 최적 생장은 주로 10~15℃에서 일어나게 된다.Sesame seeds generally grow and adapt to the conditions where the temperature decreases after passing 23 ~ 24 ℃. If the growth temperature is 20 ℃ or more spore release is active and the leaf growth is falling, the optimum growth occurs mainly at 10 ~ 15 ℃.

한편, 12주 배양하는 동안 야생형(W)의 생장을 살펴보았을 때, 엽장은 온도가 높아질수록 생장이 낮아졌으며, 엽폭은 10, 15℃에서 높았던 반면 5, 20℃에서 낮았다. 또한, 엽체 형태는 온도가 높아질수록 세장형에서 원형으로 변하였다. 12주 배양하는 동안 신품종(LB)의 생장을 살펴보았을 때, 8주까지는 10, 15, 5, 20℃ 순이었으나 그 이후에는 15, 10, 5, 20℃ 순이었다. 엽폭은 15℃에서 가장 높았으며 5, 10℃는 유사하였고 20℃에서 가장 낮았다. 엽체 형태는 10℃에서 가장 높았으며 5, 15, 20℃ 순으로서, 5~15℃에서는 세장형이었으나 20℃에서는 원형을 나타내었다.On the other hand, when looking at the growth of wild-type (W) during the 12-week cultivation, the growth was lower as the temperature increases, the leaf width was higher at 10, 15 ℃ while lower at 5, 20 ℃. Also, the leaf morphology changed from elongate to circular with increasing temperature. When the growth of new varieties (LB) was observed during the 12-week incubation, it was 10, 15, 5, 20 ℃ in order up to 8 weeks, but after that, 15, 10, 5, 20 ℃. Leaf width was highest at 15 ℃ and similar at 5 and 10 ℃ and lowest at 20 ℃. The leaf shape was the highest at 10 ℃ and was in the order of 5, 15 and 20 ℃. It was elongated at 5 ~ 15 ℃ but circular at 20 ℃.

신품종(LB)은 야생형(W)에 비하여 5℃를 제외하고 10, 15, 20℃에서 엽장 생장이 빠른 특징을 나타내었으며, 엽폭은 5℃와 20℃에서 야생형(W)보다 조금 더 넓게 생장한 반면, 10℃와 15℃에서는 좁았다. 엽체 형태를 나타내는 엽장/엽폭 조사한 결과 신품종(LB)은 5℃를 제외한 10, 15, 20℃에서 4.66~5.88의 높은 값을 나타냄으로써 야생형(W)에 비하여 세장형을 나타내었다.The new varieties (LB) showed faster growth at 10, 15, and 20 ° C than 5 ° C compared to wild type (W), and the leaf width was slightly wider than wild type (W) at 5 ° C and 20 ° C. On the other hand, it was narrow at 10 ℃ and 15 ℃. As a result of the leaf / leaf width of the leaf type, new varieties (LB) showed an elongated form compared to wild type (W) by showing high values of 4.66-5.88 at 10, 15, and 20 ° C except 5 ° C.

이상의 결과를 종합하면 신품종(LB)은 야생형(W)보다 10~20℃에서 엽장 생장이 빠르고, 세장형인 새로운 품종임을 확인할 수 있었다.In summary, the new varieties (LB) was faster than the wild type (W) at 10-20 ℃ the growth rate of the leaves, was confirmed that the new breed is elongate.

Claims (2)

순계화 방법으로 개발되어 수온이 10~20℃에서 엽장 생장이 빠르고, 엽체 형태를 나타내는 엽장/엽폭은 10~20℃에서 4.66~5.88의 높은 값을 나타내는 세장형이고, 엽장의 생장은 12주 배양시 18.0~104.4 mm로 생장하는 것을 특징으로 하는 신품종 참김.Developed by the netting method, the leaf growth is fast at 10 ~ 20 ℃, and the leaf / leaf width which shows the shape of the leaf is elongate showing the high value of 4.66 ~ 5.88 at 10 ~ 20 ℃, and the growth of the leaf is 12 weeks culture. New varieties of patissier characterized by growing at 18.0-104.4 mm. 삭제delete
KR1020100021674A 2010-03-11 2010-03-11 New varieties Porphyra tenera of slender-type by pure line method KR101092964B1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
KR1020100021674A KR101092964B1 (en) 2010-03-11 2010-03-11 New varieties Porphyra tenera of slender-type by pure line method

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
KR1020100021674A KR101092964B1 (en) 2010-03-11 2010-03-11 New varieties Porphyra tenera of slender-type by pure line method

Publications (2)

Publication Number Publication Date
KR20110102590A KR20110102590A (en) 2011-09-19
KR101092964B1 true KR101092964B1 (en) 2011-12-19

Family

ID=44954148

Family Applications (1)

Application Number Title Priority Date Filing Date
KR1020100021674A KR101092964B1 (en) 2010-03-11 2010-03-11 New varieties Porphyra tenera of slender-type by pure line method

Country Status (1)

Country Link
KR (1) KR101092964B1 (en)

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
Aquaculture 274:126-131 (2008)

Also Published As

Publication number Publication date
KR20110102590A (en) 2011-09-19

Similar Documents

Publication Publication Date Title
Nassar et al. Cassava improvement: challenges and impacts
JP2007512025A (en) System and method for aquaculture of seaweed and other seaweeds in land-based seawater tanks
JP6043117B2 (en) New tomato multi-tailored grafted seedling and method for producing the same
KR101184318B1 (en) New cultivar of Porphyra yezoensis Ueda Jeonnam super gim 1ho
WO2019211736A1 (en) Non-gm improved tomato crops and methods for obtaining crops with improved inheritable traits
JP2015507938A (en) Cultivated Oryzasativa plants having a partial or complete multigenome and uses thereof
CN104620969A (en) Rapid breeding method of Sargassum fusiforme
KR20140038353A (en) Fruit formation in the absence of fertilisation
Roh Controlled flowering in the genus Lilium-review of the past achievements and the future direction of research
KR101001370B1 (en) Method for rearing new cultivar of porphyra yezoensis ueda and new cultivar of porphyra yezoensis ueda produced by the same
KR100492518B1 (en) A new species of plant BAEMOOCHAE and method for breeding the same
CN106665365B (en) A kind of heat-resisting Japonica two line hybrid rice new method
CN106879473B (en) A kind of method that larch Vitro Plant occurs
KR101092964B1 (en) New varieties Porphyra tenera of slender-type by pure line method
CN104823923B (en) A kind of mating system for preventing aphidius gifuensis population deterioration
Chen et al. Studies on colchicine-induced autotetraploid barley. I and II. Cytological and morphological observations
CN103210834A (en) Method for breeding non-glutinous rice and glutinous rice varieties through non-glutinous rice and glutinous rice hybridization
CN103999680B (en) Plasma treatment willow branch produces the method for bud mutation
KR101515542B1 (en) New Strawberry Variety Jukhyang and the Method for Breeding the Same
KR101239500B1 (en) Method for rapid multiplication of high quality yam plug seedlings
Jindal et al. PSM-1: Ahigh yielding bell pepper variety having seed production potential under North Indian plains
CA3003982C (en) Non-gm improved crops and methods for obtaining crops with improved inheritable traits
Saruwatari et al. Interspecific lily hybrids with the ability to flower precociously and to produce multiple flower stalks from Lilium formosanum
KR101983724B1 (en) New Thermophilic Strain of P. dentata (Kjellman) N. Kikuchi, M. Miyata
Luthra et al. Evaluation and selection of true potato (Solanum tuberosum) seed families in North-Central plains of India

Legal Events

Date Code Title Description
A201 Request for examination
A302 Request for accelerated examination
E902 Notification of reason for refusal
E701 Decision to grant or registration of patent right
GRNT Written decision to grant