KR101983724B1 - New Thermophilic Strain of P. dentata (Kjellman) N. Kikuchi, M. Miyata - Google Patents

Abstract

The present invention relates to a novel thermophilic porphyra dentata, which can contribute to an increase in the income of fishermen and a laver-related industry. According to the present invention, the novel thermophilic porphyra dentata is deposited with accession number KCTC18673P, and has a higher leaf grow rate at 25°C than 20°C or less.

Description

{New Thermophilic Strain of P. dentata (Kjellman) N. Kikuchi, M. Miyata}

The present invention relates to a new type of high temperature intrinsic mottle flounder excellent in growth of leaves at high temperature.

A variety of new varieties have been developed to increase productivity and quality in Korea, Japan, and China. The production of Korean aquaculture has increased from 200,000 tons to 410,000 tons in 2014 And the export value is more than $ 300 million (as of 2015).

In the domestic pyropia tenera [Pyropia tenera (Kjellman) N. Kikuchi , M. Miyata, MS Hwang & HG Choi], the radiation pattern Kim [P. yezoensis (Ueda) MS Hwang & HG Choi], all pattern Seaweed [P. seriata ( Kjellman, N. Kikuchi, M. Miyata, and P. dentata (Kjellman) N. Kikuchi and M. Miyata. Especially, it has about 70 ~ 80% of the radiant pattern which has a lot of pores and strong flavor.

It is known that appetite rot is favored by aquaculture farmers as an industrial high value species because it has good taste, incense, texture and color and is traded at a high unit price at the early stage of farming. These buddhist rotifers are native early cultivars of Korea and grow at the early stage of the cultivation. Because they do not release single spore, they can not be continuously produced after 1 to 2 times of collection. Output and price are unstable.

According to the research report of the National Fisheries Research and Development Institute ("Improvement of Rice Varieties"; Hwang Mi-Sook, 2009), the growth rate of the rhizome at 15 ℃ is the fastest at 10 ℃ (Fig. 1). Most of the varieties, such as radishes and roots, have inhibited leaf growth at 20 ℃. This is a characteristic of the Kim variety that corresponds to the fact that the harvesting of the cultivated roots is carried out in the winter season from December to March. If high - temperature - tolerant rice varieties with excellent leaf growth at high temperature are developed, it will be possible to harvest the kimchi in the summer season.

According to recent reports (end of 2016), the surface water temperature in Korea is 1.39 ℃ in the East Sea, 0.91 ℃ in the South Sea, and 1.20 ℃ in the Yellow Sea from 1968 to 2015 ℃ and 1.11 ℃, the water temperature around the year 2100 can be 4 ~ 6 ℃ higher than 2000.

The higher the seawater temperature is, the lower the growth and the disease, and the lower the yield will be expected.

"Improvement of Rice Varieties: Searching for Good Varieties and Selective Breeding" Journal of the Korean Fisheries Society, 2009

It is an object of the present invention to provide a novel Kim variety having high temperature tolerance and excellent leaf growth at a high temperature.

In order to accomplish the above object, the present invention relates to a new high-temperature resistant new variety varieties [deposit number: KCTC18673P].

The new Kimchi varieties according to the present invention are characterized by having a higher leaf growth rate at 25 占 폚 than at 20 占 폚 or lower.

The new variety of Kimchi according to the present invention is characterized by having the following Kimchi characteristics:

(a) Leaf shape: Linear

(b) Mean length, width and thickness of leaf lobes: 30.2, 3.8 cm, 83 탆

(c) Middle and lower color of foliage: black green, yellow green

(d) Single spore release: none

(e) Shell crumb color: red purple

(f) Mounting of foliage: Yes

(g) Reproduction type of leaves: male and female.

Further, the present invention relates to a kim or a Kim by-product made of the above-mentioned Newborn fledgling variety material.

As described above, the new varieties according to the present invention [Accession No .: KCTC18673P] exhibits the highest growth rate at a high temperature of 25 ° C or higher, whereas the conventional obturator bulb shows the highest growth rate at around 15 ° C.

Accordingly, the new varieties of the present invention are not only economically viable as varieties of Kim corresponding to the rise in sea water temperature due to global warming, but also can be produced during the year beyond the conventional rice cultivation period in the winter season, It will make a great contribution to the Kim industry.

Fig. 1 is a graph showing the growth rate (foliage) of the indoor cultivating buds by temperature.
Fig. 2 is a photograph of a shell crust of a new varieties of obtuse birds according to the present invention. Fig.
Fig. 3 is a photograph of a spore sac and a leaf of Kimbil in a new varieties of the obovate according to the present invention.
Fig. 4 is a photograph of a shell-like body of a new brand-new work piece according to the present invention and a general artist's work piece.
Fig. 5 is a photograph of a petal of a new varieties of obtuse birds according to the present invention.
FIG. 6 is a microscope photograph showing a stationary protrusion of a new type of body floss according to the present invention. FIG.
FIG. 7 is a photograph showing the top and bottom colors of a green leaf of a new name variety according to the present invention. FIG.
Fig. 8 is a photograph showing the shape of a new type of an obturator flap according to the present invention and a general obturator flap;
Fig. 9 is a photograph showing the shape of a new varieties of the obtuse birds according to the present invention and the common obtuse birch.
Fig. 10 is a graph showing the growth of the new varieties of the present invention with temperature according to temperature.

DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS Hereinafter, the present invention will be described in detail with reference to the accompanying drawings and embodiments. However, the drawings and the embodiments are only illustrative of the contents and scope of the technical idea of the present invention, and the technical scope of the present invention is not limited or changed. It will be apparent to those skilled in the art that various changes and modifications can be made within the scope of the technical idea of the present invention based on these examples. Also, when reference numerals are used in the drawings, the drawings are used only for illustration purposes and are not intended to limit the elements.

The new varieties according to the present invention are obtained by collecting the leaves of the pine needles which have good coloring in the islands of the west coast, proliferating the pine needles, inducing mutation, and selecting the leaves having excellent growth rate. The selected leaf body has a different characteristic from that of conventional artificial buds, and unlike conventional artificial buds, it has the highest growth rate at a high temperature of 25 ° C or higher. Therefore, the present inventors designated this as "Gold No. 3" in the name of "Itbadudo" and deposited it with the deposit number KCTC18673P at the Life Resource Center of the Korea Research Institute of Bioscience and Biotechnology.

[Example]

1. Selection of the best leaves

We collected Kimbok which has good coloring in Koyang Island rocks in Gunsan City, Jeonbuk Province. As a result of species analysis, the collected Kim was identified as Pyropia dentata (Kjellman) Kikuchi & Miyata.

The harvested leaf bodies were aerated in the range of 1000 lux to 2000 lux (16: 8: L: D) according to a conventional method. In order to prevent infestation of the foliage, it was converted into a three-day cycle.

Spongiospores were collected from the collected spores. Gamma rays were irradiated at 1KGy on a gamma ray obtained from Korea Atomic Energy Research Institute (KERI) located in Jeongeup, and then spores were released under the conditions of water temperature 23 ℃ and 1000lux ~ 2000lux (16: 8: L: D).

Ten well - grown leaves were separated and cultured in two ventilated incubators with 5 experimental groups. In one of them, the formation of rumen was observed. The formation of obturator flame means that the female gametophyte and male gametophyte have been modified.

The process of inducing the scar from the spore formed above and isolating only one strand of the formed strands of colony and culturing for 15 days was repeated to finally select the pure strain.

2. Confirmation of the suitability of selected buddhist

The same test form process as that of the ordinary Kim style was conducted to confirm that the above-mentioned net rudder floss with excellent growth rate was suitable for the actual style.

First, the selected preliminary glass scrap was mass-cultured at 20 ° C under 300 lux to 800 lux (14: 10: L: D). Next, approximately 18,000 shells were attached to the 3m × 10m tank of the Seonjin Fishery Shell Culture Center located in Maejiri, Seochon-gun, Chungnam Province in January 2016 (first) and January 2017 (second) (Fig. 2). In Fig. 2, A is an early stage of incubation of shell crust, B is a middle phase culture of shell crust, and C and D are fluorescence microscopic photographs of crustal intrusion.

In late August, 2016 (1st) and August, 2017 (2nd) in 2019, the seedlings were sown in Jinjin Fishery Co., Ltd.

As can be seen from the figure, after 1 week of harvesting, leaves did not fall off but grew vigorously at Kimbal in outdoor farms. In Fig. 3, A is the obtuse spindle spore, B is the spore immediately after germination, C is 5 days after seedlings, and D is 10 days after seedlings (Scale bar: AB: 50 μm , CD: 200 μm ).

3. Characteristic analysis of selected bodybuilding purse

(1) Comparison of traits

At the same time, the quality of Kimbok was investigated during the process of conformity assessment.

① According to the KS standard color, the shell of a new kind of shell of the present invention is reddish purple (see FIG. 4), which is brighter than that of the existing one.

② Leaf shape of new varieties is linear and length and leaf width of leaves are longer than existing varieties (Fig. 5).

③ There is a mount on the side of the foliage of new varieties (Fig. 6).

④ New variety The middle part of the foliage is black green and the bottom is yellow green (Fig. 7).

(2) Comparison of characteristics and form of new varieties and control varieties according to the present invention

The new varieties and control varieties were collected two times in the process of confirming the suitability of the varieties and their characteristics (Table 1 ~ 3 below) and morphology (Figures 8 ~ 10) were compared.

[Table 1] Characteristic characteristics of 13 traits of the new varieties of the ungermic flounder according to the present invention

As can be seen from the above Tables and figures, it was confirmed that the new variety according to the present invention is significantly superior to the control varieties in terms of morphological characteristics but also in leaf size.

(3) Investigation of leaf growth rate of new varieties according to the present invention

The length and width growth of new cultivars were examined under the culture conditions of 1000 lux to 2000 lux (16: 8: L: D) at the respective water temperatures of 10, 15, 20 and 25 ℃ with the prepared ventilator (Table 2 10).

[Table 2] Growth characteristics (leaf length length cm)

[Table 3] Growth characteristics (leaf width cm)

The length of foliage was the highest at 25 ℃ at 25 ℃, 24 ± 1.9cm at 21 ℃, 21 ± 3.2cm at 20 ℃, 17 ± 2.4cm at 15 ℃ and 8 ± 1.9cm at 10 ℃. The width of the leaves was also 7 ± 1cm at 25 ℃ and the fastest growth was 3.2 ± 1.2cm at 10 ℃, 4 ± 0.9cm at 15 ℃ and 6.2 ± 0.6cm at 20 ℃.

This shows that the new varieties of this embodiment of the present invention can grow rapidly at 25 ° C and 27 ° C, which is the seawater temperature at the end of August. As shown in FIG. 1, the length of the leaves is about 0.8-4.5 cm at the 6-week course at 15 ° C, at which the ear bud bud is best developed. On the other hand, the new variety according to the present invention, The length was about 10 cm and showed good growth rate in high temperature seawater.

Claims (4)

New varieties of high temperature tolerance deposits deposited with deposit number KCTC18673P.
The method according to claim 1,
A new varieties of high temperature resistance resistant new bodied rhododendrons, characterized in that the leaf growth rate at 25 ° C is higher than at 20 ° C or lower.
The method according to claim 1,
New varieties of high-temperature-tolerant new deep-bodied flora characterized by having the following characteristics:
(a) Leaf shape: Linear
(b) Mean length, width and thickness of leaf lobes: 30.2, 3.8 cm, 83 탆
(c) Short spore release: None
(d) Foliation:
(e) Reproduction type of leaves: male and female. delete

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