KR101001370B1 - Method for rearing new cultivar of porphyra yezoensis ueda and new cultivar of porphyra yezoensis ueda produced by the same - Google Patents

Abstract

PURPOSE: A method for raising novel species of Porphyra yezoensis is provided to yield novel species having wider leaf width than general laver and to produce cultured laver having soft texture and specific flavor. CONSTITUTION: A method for raising novel species of Porphyra yezoensis comprises: a step of culturing parental thallus of Porphyra yezoensis from 15°C to 20°C for 24 hours; a step of removing the parental thallus and culturing; a step of separating seed and sub-culturing; a step of culturing in a medium containing PES and sterilized seawater at 15°C in a bright place for 14 hours and dark place for 10 hours; a step of culturing Conchocelis phase from horizontal type to hanging type; and a step of culturing under 1,500 lux from April to May, 700-1,500 lux from mid May to June and 500-1,000 lux from July to August.

Description

Method for nurturing new varieties of radish patterned laver and new varieties of radiant patterned laver {Method for rearing new cultivar of porphyra yezoensis Ueda and new cultivar of porphyra yezoensis Ueda produced by the same}

The present invention relates to a new type of radiation pattern laver developed by breeding through the filamentous filamentous culture and shell filamentous cultivation by collecting the large leaf of the radiation pattern laver excellent in traits.

The seaweed belongs to the taxonomy of red algae, red algae, primrose red sea, gimparaemok, gimparae and gimsok. There are four types of aquaculture, radial patterned seaweed, sesame and seaweed, seaweed and seaweed. In 2008, Korea's annual output was 220,000 tons (24.4% of seaweed production) and 194 billion won (57.7% of seaweed production). It is the most important seaweed in the industry. The most abundant of these, and the one that has the greatest ripple effect on the laver industry is radiant laver. In particular, since the introduction of artificial seedlings, radial patterned laver with excellent environmental stress has become the dominant species. In Japan, the situation is similar to that of Korea, and after the introduction of artificial seedlings, the radiation pattern laver became dominant not only in the farm but also in the natural population. Radiation pattern laver is traditionally farmed laver in Korea, which is soft and has no holes, so it is processed into dry laver and is distributed as laver laver and laver.

In 2012, the new plant protection system under the UPOV (International Plant Protection Species) will be implemented in the marine plants (seaweed) field. Among the three countries, a number of varieties have been developed in Japan and distributed in Korea and China as filaments. Especially, in Japan, the elongated form of radish laver is imported. In order to prepare for the new breed protection system, also called seed war, it is necessary to hurry to develop domestic varieties.If developing new varieties using various breeding techniques, it is possible to lower royalty payments for Japanese varieties and further generate royalty income through export of domestic varieties. I think it will be.

Accordingly, the Haenam branch of Fisheries Technology Office, Jeollanam-do, started breeding on radiant laver from '08, and the result of the test was better than that of the current aquaculture. However, as for the algae, there is still a lack of cultivation criteria, so the new varieties are protected as patents.

As a related patent application, Korean Patent Publication No. 10-0356102 discloses new varieties of laver and its hybridization method by interbreed of large sesame and large radiation pattern laver, and Korean Pattern Patent Publication No. 10-0769358 Genetic differentiation methods of native varieties and primer sets used therein are disclosed.

In order to prepare for global warming and increase the income of fishers, it is urgent to breed new varieties of laver. In order to develop new varieties of radish laver, large-scale leaflets with excellent traits were collected from Song-myeon, Haenam-gun. As a result, it was possible to harvest new varieties of laver whose average leaf and leaf width were larger than those of the conventional laver, and the test results of the developed varieties showed better results than the current breeds. We proposed this as a new variety and attempted to register the breed. However, in Korea, the criteria for reviewing the breed have not been established yet. From this, by developing a new breed of radiant patterned seaweed with excellent traits, it is possible to produce cultured seaweed with its unique soft touch and unique flavor, and the produced seaweed can be processed into seaweed laver as well as traditional seaweed.

In order to develop new varieties of radish laver, we collected and selected large leaflets with excellent traits at the Sanjeongri branch of Song-myeon, Haenam-gun.

The growth rate of filamentous filamentous filament was faster than filamentous laver, and the spores of normal lattice laver released around 20 ℃, but the release time of new varieties of spores was around 24 ℃. This means that spores are released at high water temperatures, so early farming is possible. In addition, new varieties of laver can be sold at high prices because they have better ability to overcome pests than other laver.

Therefore, according to the development of domestic varieties, royalty payments to Japanese varieties can be lowered and, furthermore, royalty income can be generated through exports of domestic varieties. It is judged that the new plant protection system can be prepared for implementation.

Figure 1 Mother leaf
Fig. 2 Glass filamentous culture
Figure 3 submerged shell filamentous culture
Figure 4 Preparation for the sale of the humus-treated shell
Figure 5 Shell Formation for Test Form
Fig. 6 Maritime envelope type seedlings (9.23)
Fig. 7 Sapling confirmation (9.28)
Fig. 8 Lobe attached to gimbal
9 Microscope (× 40) of the leaf
10 Comparison between normal laver and new species laver
Fig. 11 Shell Formation Immediately Before Harvesting (Humidity Treatment)
Figure 12 Leaf color comparison between normal radish laver and new varieties laver (usually radiant laver: brown or dark brown, new laver laver: dark green or black green)

The present invention relates to a new type of radiation pattern laver, characterized in that the leaf length of the radish laver is 12,1 to 34.3 cm, the leaf width is 2.8 to 18.6 cm, and the growth is fast and the leaf color is dark green or black green. As a result, new varieties of laver with higher mean and leaf width than conventional laver could be harvested. More detailed breeding process is as follows.

1. New breeding process

1) Collecting parental thallus :

On March 20, 2008, large leaflets with excellent traits were collected and selected to develop new varieties of radiation-pattern laver. At this time, the collected leaf size was 108 cm long and 79 cm wide.

2) Cultivation of glass filaments : '08. 5 ~ present

In total, the cells of the mother leaf body were not mature, and then put the leaf body in a 5L Erlenmeyer flask for 7 to 10 days, and aeration culture was performed by increasing the water temperature from 15 ° C. to 20 ° C. at 500 Lux and 24 hours of light period. . After microscopic confirmation of the maturation of the cells, stationary culture was performed.

Microscopy confirmed the release of superspores and neutrophils from the stationary culture, and then the parental body was removed and subjected to stationary culture again.

After 30 days, it was visually confirmed that the spores adhered to the bottom of the saale and grew into a glassy filament, and then removed using a Pasteur pipette and precision tweezers, and then cultured in a new 500 mL Erlenmeyer flask.

The growth conditions were cultured in a culture medium in which sterile seawater and PES medium were added to the glass filaments and adjusted to a water temperature of 15 ° C., an illuminance of 500 Lux, a photoperiod of 14 hours, and a flash memory of 10 hours.

3) Shell carcass culture : '09. 4. 13 ~ 9. 15 (155 days)

The cultured glass filaments were rotated for 20 to 30 seconds with a blender and shredded to 300-500 µm. The glass filaments were sparged with 0.01 g of one shell (50 shells) to start culture of shell filaments.

After incorporating the glass filament into the shell after 20 days, the glass filament was immersed in the shell, and in the planar culture state, the hydrangea was produced and changed to the drop cultivation.

During the incubation period, the roughness and water temperature management were controlled at about 500 Lux or less for 2 days at the beginning of glass filament immersion, and then the water temperature was 13 ~ 16 ℃ and the illuminance was less than 1,500 Lux until mid-April to May. The water temperature was maintained at 16 ~ 25 ℃, roughness 700 ~ 1,500 Lux, and July ~ August at 25 ~ 30 ℃ and roughness 500 ~ 1,000 Lux. In addition, the change of culture seawater was carried out every 15 to 30 days.

4) Chapel Shell  Pretreatment

After incubating the shells, the shells were pretreated before seeding. The treatment method was a combination of humidity treatment and dark treatment. The treatment period was carried out for 7 days from September 15 to 22, 2009.

After preliminary test of the release of each spore at the water temperature of 24 ℃, after confirming the release status, the shells were transferred to Haenam branch culture room of Fisheries Technology Office, Jeollanam-do, and 100% humidity treatment and dark treatment were carried out simultaneously to promote the maturation of the spores and suppress the release of each spore. It was stored in the bag until the day of the seedling.

5) Seedlings

The marine seeding was carried out in the sea bag type, and the shell requirement was 4 boxes (2.2 × 40m) per book (200 shells). Seedling days of each test ground are as follows.

-Volcanic Mt. Marge Line (Classic Type): '09. 9. 23

-Sulfate Oxygen Branch (holding stock): '09. 9. 29

-Songmyeon fishery line (type): '09. 9. 30

6) Spores With net  Confirm

After 7 days of harvesting, 10 cm of the test company was cut and transported in Kim Mang, and the germination status of each spore was confirmed by a fluorescent microscope in a laboratory.

7) Training Management

After harvesting, Kim Mang was grown for 0.5 ~ 1㎝ in nursery for 20 ~ 30 days and transferred to the nursery. Three hours / day exposure was controlled at the aquaculture farm and 10 hours / day exposure at the aquaculture farm.

The fishing environment, such as the average water temperature and specific gravity of the test farm, is shown in the following table.

Average water temperature and specific gravity of the test farm division
Water temperature (℃) Specific gravity (1.0-) September October November December September October November December this year 23.50 19.80 14.30 10.3 23.6 24.1 25.7 26.2 Average year 23.50 19.40 14.70 10.2 22.8 24.0 24.6 25.5 Average annual 0 +0.4 -0.5 +0.1 +0.8 +0.1 +0.4 +0.7

※ Salinity concentration 32 ~ 33 ‰ during farming, pH 8.1 ~ 8.4

8) Growth Survey

In order to measure the traits of cultured seaweed, 30 fish were harvested at random from each fishery and variety. The harvesting of seaweed in Haenam was first performed 60 to 70 days after harvesting, and then collected at intervals of 20 to 25 days.

The average leaf and leaf width of seaweed collected by each fishery are as follows.

(A) Songmyeon roe: 11. 25

-New varieties of laver: Average leaf length 27.03㎝ (13.6 ~ 51㎝), leaf width 10.97㎝ (4.6 ~ 18.6㎝)

-Normal laver: Average leaf length 17.87㎝ (13 ~ 25.8㎝), leaf width 5,37㎝ (2.8 ~ 7.9㎝)

(B) Volcanic cotton jungma: 12. 10

-New varieties of laver: Average leaf length 18.42㎝ (12.3 ~ 34.3㎝), leaf width 6.95㎝ (4.1 ~ 11.7㎝)

-Normal laver: Average leaf length 9.48㎝ (5.3 ~ 18.4㎝), leaf width 1.89㎝ (0.9 ~ 3.4㎝)

(C) Surface sulfate oxygen: 12.9

-New varieties of laver: Average leaf length 19.1㎝ (12.1 ~ 30.3㎝), leaf width 7.34㎝ (2.8 ~ 16.7㎝)

-Normal laver: Average leaf length 10.16㎝ (5.7 ~ 19.9㎝), leaf width 1.7㎝ (0.7 ~ 3.6㎝)

Comparing the average leaf growth of the new varieties of laver and normal laver collected from these three branches, the average leaf length of the new varieties was 21.52 cm and the leaf width was 8.42 cm, while the average leaf length of the general laver was 12.5 cm and the leaf width was 2.99 cm. In comparison with the difference in growth of two varieties, the new varieties of laver were 9.02㎝ in leaf length and 5.43㎝ in width.

In addition, comparing the price of the consigned sale of the collected seaweed as follows.

-Fish nest laver (11.25): New varieties of seaweed 55,000 won / 60㎏, average laver 35,000 won / 60㎏

-Volcano Stomach Market (12.14): New species seaweed 88,000 won / 60㎏, average seaweed 60,000 / 60㎏

-Sulfuric Acid Stomach Market (12.19)

In other words, new varieties of seaweed are sold at a high price of about 17,000 ~ 28,000 won compared to general laver, and it has been found that they are favored by consumers.

2. New varieties of laver  form Radiation pattern  difference

1) Morphological differences between shells and the release timing of spores

The differences between the new varieties of laver and cultured radish laver are as follows. Fig. 11 shows the shell shaped body just before the seedling (humidity treatment).

Comparing the growth rate of shell shells, the growth rate of filamentous bodies was faster than that of shell shells put in early (February-March) even though the glass shells were in late (April).

In addition, the shells of radiant laver were initially gray and gradually turned black, and the color gradually became lighter when it was released. (Figure 11)

In general, the spores of the radiation pattern laver is released around 20 ℃, the release time of the new varieties of spores around 24 ℃, the release time is fast. This means that spores are released at high water temperatures, so early farming is possible.

2) Morphological differences in the leaf body

In the case of laver leaves, radiation pattern laver was collected around October 20 every year. However, in 2009, red leaf disease was so severe that the lamellar leaves were severely defoliated and the quality was poor. But new varieties of seaweed overcame the disease during the disease season. This suggests that the ability to cope with pests is better than that of other seaweeds and therefore can be sold at high prices. In addition, it grew 1.5 to 2 times faster than other radish lavers drawn at the same time, and the normal laver lattice was brown or dark brown, whereas the color of the leaves of new varieties was dark green or black green (Fig. 2). . Gray green, dark green, or black green is the Munsell symbol and corresponds to colors in the range of 4G-5G.

Claims (9)

Performing aeration (air injection) culturing for 24 hours while raising the water temperature gradually from 15 ° C. to 20 ° C. in the parental thallus of the radiation pattern laver selected from the natural group;
Confirming the release of the superspores and the neutral (single) spores from the mother leaf during the incubation, removing the mother leaf, and performing the stationary culture;
After 30 days after confirming that the spores grew into glass filaments, the glass filaments were removed and subcultured to derive the glass filaments from the mother leaf;
Culturing the glass filaments in an incubator adjusted to 15 ° C., specified 14 hours, and memorized 10 hours in a culture solution to which sterile seawater and PES medium were added;
Start the culture of the Conchocelis phase by spraying a thin glass filament on the shell for 20-30 seconds with a blender.After checking the infiltration of the glass filament on the shell, change the shell from horizontal to hanging. As a step of culturing filaments, the initial roughness of the glass filament infiltrated into the shell was controlled for 48 hours at 500 lux or less, and then 1,500 lux or less until mid-April-May, 700-1,500 lux by mid-May-June, Maintaining 500-1,000 lux by July-August; And
New breeding method of the radiation pattern laver including the seedling step.
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