KR101086504B1 - Composition applicable to skin and capable of improving blood circulation and enlarging blood vessels - Google Patents

Abstract

The present invention (1) licorice (licorice root) extract, Curcuma longa extract, Grape extract, Grape seed oil, grapefruit extract, Bamboo extract, Raspberry extract, Cinnamon extract, At least one extract selected from the group consisting of Hovenia dulcis extract, Japonica extract, Alder extract or mixtures thereof; (2) at least one member selected from the group consisting of ammonia, nitrite, nitroglycerline, silver nitrate, magnesium nitrite, ammonium nitrate or mixtures thereof; And (3) malic acid, hyaluronic acid, citric acid, citric acid, vitamin C, tocopherol, tocopherol, aspartic acid, tartaric acid, succinic acid, retinol (retinol), glutamic acid (glutamic acid) and the present invention relates to a composition for skin application having a blood circulation improvement and vasodilation function comprising at least one selected from the group consisting of additives. The invention also relates to a method for improving blood circulation and dilatating blood vessels comprising applying the composition to the skin.

cAMP, cGMP, Adenylyl cyclase, guanylyl cyclase, improved blood circulation, vasodilation, skin application

Description

Composition for skin application with improved blood circulation and vasodilation {Composition applicable to skin and capable of improving blood circulation and enlarging blood vessels}

The present invention (1) licorice (licorice root) extract, Curcuma longa extract, Grape extract, Grape seed oil, grapefruit extract, Bamboo extract, Raspberry extract, Cinnamon extract, At least one extract selected from the group consisting of Hovenia dulcis extract, Japonica extract, Alder extract or mixtures thereof; (2) at least one member selected from the group consisting of ammonia, nitrite, nitroglycerline, silver nitrate, magnesium nitrite, ammonium nitrate or mixtures thereof; And (3) malic acid, hyaluronic acid, citric acid, citric acid, vitamin C, tocopherol, tocopherol, aspartic acid, tartaric acid, succinic acid, retinol (retinol), glutamic acid (glutamic acid) and the present invention relates to a composition for skin application having a blood circulation improvement and vasodilation function comprising at least one selected from the group consisting of additives. The present invention also relates to a method for improving blood circulation and dilatating blood vessels comprising applying the composition to the skin.

In the human body, blood circulation is regulated by a variety of factors, including physical factors such as hematocrit, viscosity, and shear stress caused by the blood, as well as changes in blood lipid composition and blood cells. (Platelets) and the like. These various factors maintain homeostasis well under normal conditions, but when blood circulation control functions are disturbed by diseases such as diabetes, drugs, and smoking, excessive aggregation of plasma, platelets, red blood cells, and abnormal haemostasis occurs. Blood flow is impaired.

Blood circulation improvers restore these inhibited blood flows to normal conditions, preventing aging of cells and organs, and promoting cell growth and regeneration, resulting in stiff shoulders and neck, limbs, cold legs, and scalp caused by peripheral blood circulation disorders. Reduces hair loss symptoms due to blood circulation disorders. However, although various medicines are being developed to improve blood circulation, most of the products are oral preparations, and the inconvenience of having to stop taking them due to symptoms such as stomach discomfort, diarrhea, constipation, anorexia and bloating Occurs.

On the other hand, if adenylyl cyclase (Adenylyl cyclase) is activated, cAMP production is increased and cAMP eventually activates protein kinase A (Protein kinase A), protein kinase A inhibits Rho kinase (Rho kinase) activity. As a result, myosin phosphatase is activated, dephosphoralation of myosin occurs, and the binding of myosin and actin is lost, thereby smoothing smooth muscle.

In addition, when soluble guanylyl cyclase is activated, cGMP production is increased and cGMP eventually activates protein kinase G, which activates myosin phosphatase. The combination of myosin and actin breaks down, smoothing muscles.

Therefore, a substance that artificially activates adenyl cyclase and guanyl cyclase proteins to induce cAMP and cGMP production can be used to alleviate smooth muscle. However, among the various oral foods and medicines currently developed for improving blood circulation, a substance that can be assured of efficacy is rare, and there are disadvantages in that it takes the inconvenience and side effects of long-term use.

Under this background, the present inventors have found that the blood circulation disorder and peripheral vascular dysfunction, cold hands and feet, Raynaud's phenomenon, erectile dysfunction, impaired sexual sensation, scalp without causing side effects that occur in oral blood circulation improvers. As a result of efforts to develop a skin coating composition that can improve the symptoms of hair loss caused by blood circulation disorders, cAMP and cGMP are artificially activated by activating adenylyl cyclase and guanylyl cyclase proteins. The present invention has been developed a composition exhibiting blood circulation improvement and vasodilation activity through the mechanism of inducing production, thereby completing the present invention.

One object of the present invention is (1) licorice (licorice root) extract, curcuma longa extract, grape extract, grape seed oil, grapefruit extract, bamboo extract, raspberry extract, cinnamon ( One or more extracts selected from the group consisting of Cinnamon extract, Hovenia dulcis extract, Japonica extract, Alder extract or mixtures thereof; (2) at least one member selected from the group consisting of ammonia, nitrite, nitroglycerline, silver nitrate, magnesium nitrite, ammonium nitrate or mixtures thereof; And (3) malic acid, hyaluronic acid, citric acid, citric acid, vitamin C, tocopherol, tocopherol, aspartic acid, tartaric acid, succinic acid, retinol (retinol), glutamic acid (glutamic acid) and an acid for additives to provide a composition for skin application having a blood circulation improvement and vasodilation function comprising at least one selected from the group consisting of.

Another object of the present invention is to provide a method for improving blood circulation and dilatating blood vessels, comprising applying the composition to the skin.

In one embodiment, the present invention comprises (1) licorice extract, turmeric extract, grape extract, grape seed oil, grapefruit extract, bamboo extract, raspberry extract, cinnamon extract, hollyhock extract, japonica extract, alder extract or mixtures thereof At least one extract selected from the group; (2) at least one member selected from the group consisting of ammonia, nitrite, nitroglycerin, silver nitrate, magnesium nitrate, ammonium nitrate or a mixture thereof; And (3) a composition exhibiting blood circulation improvement and vasodilating activity comprising at least one selected from the group consisting of malic acid, hyaluronic acid, citric acid, vitamin C, tocopherol, aspartic acid, tartaric acid, succinic acid, retinol, glutamic acid, and an acid for additives. It is about.

The first component included in the composition of the present invention is a substance that induces cAMP production by artificially activating the Adenylyl cyclase protein, preferably licorice root extract, Curcuma longa extract , Grape extract, grape seed oil, grapefruit extract, bamboo extract, raspberry extract, cinnamon extract, honenia dulcis extract, japonica extract, alder extract or these At least one extract selected from the group consisting of: Preferably, these components may be included in 2.0 to 20% by weight based on the total weight of the composition.

Licorice (licorice root) in the present invention is a perennial herb of the Rosaceae legume, in particular the root is also used as a sweetener or medicinal. Licorice is also used for detoxification, detoxification, anti-inflammatory, relieving irritation, promoting wound healing and anti-allergy.

Turmeric (Curcuma longa) in the present invention is a perennial herb of Ginger-necked Gingeraceae, is a medicinal plant that grows and grows in Southeast Asia including India and Okinawa and is cultivated in the south-south region of Korea. It is said to have such an effect. Curcumin, a representative component of turmeric, is known to be effective in improving hyperlipidemia and reducing cholesterol concentration by promoting bile secretion in hyperlipidemia models. As a result of the inventor's analysis through in vitro experiments and computer remodeling, the curcumin which is the main raw material of turmeric extract in the present invention increases methylation by mixing the gel composition (A) and (B) of Example 1. In this case, adenylyl cyclase was further activated, and muscle relaxation was induced by breaking the bond between myosin and actin. The structural formula of curcumin is

The structural formula of the curcumin derivative induced methylation is as follows.

Grape in the present invention (Grape) is a typical alkaline food containing abundant vitamins B1, B2, C, sugars, citric acid and citric acid, and other plant fibers, such as pectin, potassium, iron. Among them, potassium helps diuretic to reduce edema and improve blood circulation, and carbohydrates are easily digested in the body, which is effective in speeding up recovery of patients after fatigue or illness. In the present invention, grape extract or grape seed oil may be used. It is also possible to use grapefruit extract with or in place of grape extract.

In the present invention, the bamboo extract lowers bad cholesterol and increases good cholesterol, which not only regulates triglycerides and blood cholesterol, but also regulates excessive blood coagulation, improving blood circulation, improving blood vessel elasticity, and damaged parts of blood. It is an effective function to regenerate.

Raspberry (Raspberry) extract in the present invention is rich in phytoestrogens, phytoestrogens, effective in preventing prostate cancer and pancreatic cancer, rich in polyphenols to hold free radicals in the body and soften blood vessels to prevent adult diseases There is no benefit to it.

In the present invention, Cinnamon (Cinnamon) is a herb belonging to the family Lauraceae, southern China and northern Vietnam is the main origin, has a lower fever, central inhibitory, platelet aggregation inhibitory, skin darkening prevention, and mainly antipyretic fever It is used for cold, analgesic, and dysmenorrhea due to aroma, stomach condition, and the effect of clay.

In the present invention, the hawthorn (Hovenia dulcis) is a broad-leaved large tree belonging to the sea buckthorn, called sesame or sesame tree, it is known that it has the effect of improving the function of the liver and to release the poison accumulated in the liver, in particular It is known to have a function to relieve hangover symptoms such as headache, dizziness, bad breath, and exhaustion after drinking alcohol.

The composition of the present invention may include an extract of the japonica family, preferably Swertia japonica extract or Alnus japonica extract. Bitter grass (Swertia japonica) is a gentian and biennial herb, refers to wild grass or bitter grass including medicinal herbs, baekryeup, and is known to have a function of gastric juice secretion or digestive juice secretion. Alnus japonica (Anus japonica) is a deciduous arborescent tree of the birch family, and is known to have a function of eliminating hangovers, releasing alcoholism and improving liver function.

The natural extract of the present invention can extract each plant using water, an organic solvent, or a mixed solvent thereof. All of these components may be mixed and extracted at the same time, or individual components may be extracted and mixed under optimum conditions.

Natural extracts used in the present invention can also be obtained by methods well known in the art, it is also available commercially. As an example of obtaining a natural extract, after drying and pulverizing the natural plant of the present invention, the pulverized product is lowered with 1 to 4 carbon atoms, such as water, methanol, ethanol and butanol, in volumes of about 2 to 20 times the dry weight. The polar solvent of the alcohol or a mixed solvent thereof may be extracted using an extraction method such as hot water extraction, cold needle extraction, reflux cooling extraction or ultrasonic extraction. In the case of hot water extraction, for example, the components of the composition of the present invention are added to water and heated at a temperature of at least 70 ° C, preferably at least 80 ° C, more preferably at least 90 ° C, for at least 2 hours, preferably at least 3 hours, The heated mixture can be prepared by filtration. When heating at a high temperature, the time is reduced, but the active ingredient is destroyed and can be lost. When heating at a temperature lower than the temperature, it is important to control the temperature and time properly because the active ingredient of the mixture is not completely extracted. The extract obtained in this way can be used after being dried or in an extracted state. When extracted using an organic solvent, alcohols such as methanol, ethanol, isoprotanol, butanol, ethylene, acetone, ether, hexane, chloroform, ethyl acetate, DMF (N, N-dimethylformamide), DMSO (dimethylsulfoxide) Side), mixtures thereof and the like can be extracted with an organic solvent at room temperature or by heating.

In one specific embodiment, the plants are collected and ground finely and refluxed with 5 to 20 volumes of water or anhydrous or hydrous lower alcohol, ethylacetate, acetone or hexane of 5 to 20 volumes by weight of the dry matter. It may be extracted by heating for 1 to 5 hours at 40 to 100 ℃ in an extractor equipped with, or by immersing at room temperature for 1 to 30 days. The extract extracted by heat extraction or cold extraction is filtered to remove suspended solid particles, for example, by filtering the particles using nylon or the like, or filtering using a freeze filtration method, and then using them as they are or using lyophilization, vacuum drying, and hot air. Drying, spray drying, etc. may be used for drying.

In addition, a solvent fraction obtained by adding water to the alcohol concentrate and suspending and fractionating using a nonpolar solvent such as hexane, chloroform or ethyl acetate may be used.

In addition, the solvent fraction is separated by silica gel column chromatography using a mobile solvent comprising a mixed solvent selected from a polar solvent such as methanol, ethanol, propanol or isopropanol and a non-polar solvent such as ethyl acetate, hexane, dichloromethane or chloroform as a mobile phase. Thus, the active ingredient with improved blood circulation and vasodilation may be used separately.

The second component included in the composition of the present invention is a substance that induces the production of cGMP by artificially activating a guanylyl cyclase protein, preferably ammonia, potassium nitrite or nitrite It is at least one selected from the group consisting of nitrites such as sodium nitrite, nitroglycerline, silver nitrate, magnesium nitrite, ammonium nitrate or mixtures thereof. Preferably, these components may be included in 2.0 to 20% by weight based on the total weight of the composition.

In addition, the third component included in the composition of the present invention is malic acid, hyaluronic acid, citric acid, citric acid, vitamin C, tocopherol, aspartic acid, aspartic acid, tartaric acid ), Succinic acid, retinol, glutamic acid and at least one acid selected from the group consisting of additive acids. Preferably, these components may be included in 2.0 to 20% by weight based on the total weight of the composition.

The composition of the present invention is a composition which can be applied to the skin, characterized in that it is preferably provided in a composition in the form of a lotion or a gel. Since conventional blood circulation improving agents are mostly oral preparations, there are inconveniences in that they should be discontinued due to symptoms such as stomach discomfort, diarrhea, constipation, anorexia, bloating, and the like. In addition, commercially available gels or lotions have a problem in that when an acid is present in the components, the raw materials are easily diffused into the air because the structure of the gels or lotions is rapidly destroyed and easily liquefied.

However, when xanthan gum is included in an amount of 0.1 to 2.0% based on the total weight of the gel or lotion as in the present invention, the solid state of the gel or lotion is maintained for a longer period of time as compared to the existing gel or lotion, and the raw material is It is stably fixed so that more active ingredients remain on the skin for a long time and can continuously penetrate into the skin.

In the present invention, xanthan gum is a pathogenic bacterium Xanthomonas Campestris is a purified and dried fermented polysaccharide that oxidizes glucose and accumulates outside the cells. , The ratio of mannose, glucose and glucuronic acid is 2: 2: 1 (see FIG. 1). Xanthan gum is a creamy or white fragrance-free powder that is soluble in water, insoluble in ethanol, ether, etc., and has a high viscosity.

The lotions or compositions of the present invention maintain their lotion or gel solid state because of their increased resistance to acids due to the presence of xanthan gum. Adding glycerin to the lotion or gel composition further enhances this effect. Therefore, in the present invention, by containing xanthan gum, or xanthan gum and glycerin, the raw material component continuously penetrates into the remaining and skin for a long time, thereby more effectively exhibiting blood circulation improvement and vasodilation activity.

In addition, animal and vegetable fats, oils, waxes, paraffins, starches, tragacanths, cellulose derivatives, polyethylene glycols, silicones, bentonites to provide compositions in the form of gels or lotions. Selected from the group consisting of silicic acid, talc, zinc oxide, Allantoin, Dipotassium Glycyrrhizate, Glycereth-26, Glucose, or mixtures thereof It may further comprise one or more.

The composition of the present invention can be applied and blended as necessary the usual ingredients to be formulated in general skin cosmetics.

In particular, the compositions of the present invention may further comprise a transdermal penetration enhancer. As used herein, the term "transdermal penetration enhancer" is a composition that allows a desired component to penetrate into the blood vessel cells of the skin at a high absorption rate. Preferably other phospholipid components, liposome components and the like used in lecithin cosmetics are included, but are not limited to these.

In addition, as the oil which can be mainly used as an oil phase component, one or more selected from vegetable oil, mineral oil, silicone oil and synthetic oil can be used. More specifically, mineral oil, cyclomethicone, squalane, octyldodecyl myristate, olive oil, Vitis binifera seed oil, macadamia nut oil, glyceryl octanoate, castor oil, ethylhexyl isononanoate, dimethicone Chicon, cyclopentasiloxane, sunflower seed oil, etc. can be used.

In addition, 0.1 to 5% by weight of a surfactant, a higher alcohol, and the like may be added to reinforce the emulsifying ability. Such surfactants may be used conventional surfactants such as nonionic surfactants, anionic surfactants, cationic surfactants, amphoteric surfactants, phospholipids, and the like, specifically, sorbitan sesquinolate, polysorbate 60 Glyceryl stearate, lipophilic glyceryl stearate, sorbitan oleate, sorbitan stearate, die-cetyl phosphate, sorbitan stearate / sucrosecoate, glyceryl stearate / polyethylene glycol- 100 stearate, ceteareth-6 oleate, arachidyl alcohol / behenyl alcohol / arachidyl glucoside. Polypropylene glycol-26-butes-26 / polyethylene glycol-40 hydrogenated castor oil and the like can be used. As the higher alcohol, alcohols having 12 to 20 carbon atoms, such as cetyl alcohol, stearyl alcohol, octyldodecanol, isostearyl alcohol, etc. may be used alone or in combination of one or more thereof.

The aqueous phase component may further add 0.001 to 5% by weight of one or more thickeners such as carbomer, xanthan gum, bentonite, magnesium aluminum silicate, cellulose gum, dextrin palmitate and the like to adjust the viscosity or hardness of the aqueous phase.

In addition, the composition of the present invention, if necessary, active ingredients such as higher fatty acids, vitamins, sunscreens, antioxidants (butyl hydroxyanisole, propyl gallic acid, elixolic acid, tocopheryl acetate, butylated hydroxytoluene). Preservatives (methylparaben, butylparaben, propylparaben, phenoxyethanol, imidazolidinyl urea, chlorphenesin, etc.), colorants, pH adjusters (triethanolamine, citric acid, citric acid, sodium citrate, malic acid, malic acid) Sodium, fmaric acid, sodium pramate, succinic acid, sodium succinate, sodium hydroxide, sodium hydrogen phosphate, etc., moisturizers (glycerine, sorbitol, propylene glycol, butylene glycol, hexylene glycol, diglycerine, Components such as betaine, glycerin-26, methylgluse-20, and the like may be further added.

In addition, the composition of the present invention further comprises a substance capable of auxiliaryly providing essential nutrients to the skin, and may preferably contain supplements including, but not limited to, natural flavors, cosmetic flavors, or herbal medicines.

The composition of the present invention can be used with confidence because there is little toxicity and side effects.

The composition of the present invention is effective in improving blood circulation and vasodilation. Therefore, the composition of the present invention can be used to improve blood circulation disorders and peripheral vascular circulation disorders, cold hands and feet, Raynaud 'phenomenon, erectile dysfunction, decreased sexuality in women, hair loss symptoms due to scalp blood circulation disorders, etc. Can be.

In addition, the composition of the present invention is effective in promoting hair growth or preventing hair loss. As used herein, the term "anti-hair loss" or "promoting hair growth" is used in the same sense, which has the same meaning as another term wool or hair growth promotion used in the art.

In a specific embodiment of the present invention, the effect of measuring the K + channel activity of the composition of the present invention using a patch clamp technique, it was found that the composition of the present invention increases the membrane current of the K + channel to activate the K + channel ( 8). This is because the composition of the present invention phosphorylates the K + channel by the activity of cAMP and cGMP and protein kinase G activity, which in turn activates the K + channel to induce a membrane current, the same mechanism as the sulfonylurea receptor (SUR) 2B K + channel activity. to be. Therefore, it can be seen that the present invention is effective in promoting hair growth or preventing hair loss through activation of the K + channel.

As another aspect, the present invention relates to a method for vasodilation and blood circulation improvement comprising applying the composition to the skin. When applied to the skin, the composition of the present invention can improve peripheral circulation and improve blood flow, thereby improving coldness of the hands and feet, and lowering body temperature.

The gel or lotion composition for skin application of the present invention does not cause side effects that occur in oral blood circulation improving agents, blood circulation disorders and peripheral blood vessel disorders, cold hands and feet, Raynaud's phenomenon (Raynaud 'phenomenon, erectile dysfunction, women's Decreased sensation, hair loss due to blood circulation disorders, etc. are effective to improve.

Hereinafter, the present invention will be described in more detail with reference to Examples. However, the following examples are illustrative of the present invention and are not intended to limit the scope of the present invention.

Example  1. Preparation of Vasodilation Lotion and Gel Composition

1-1: Preparation of Vasodilation Lotion Composition (A)

In order to prepare the lotion composition (A) according to the present invention, first, glycerin (glycerin), xanthan gum, allantoin (allantoin), dipotassium glycyrrhizate, glycerin-26 (Glycereth- 26) Glucose, Curcuma longa Extract, Grape Extract, Bamboo Extract, Hyaluronic Acid, Nitrous Acid (sodium salt), Tocopherol , Aspartic acid, Citric acid and Methylparaben were mixed in a stirrer or mixer at a predetermined ratio under aseptic conditions and then heated to 50 to 100 degrees Celsius to prepare Formulation # 1. made. In addition, hydrogenated lecithin, glyceryl stearate SE, PEG-100 stearate, cetearyl alcohol, caprylic acid, capric glyceride ), Mineral oil, grape seed oil (vitis vinifera seed oil), dimethicone (dimethicone), olive oil (olea europaea fruit oil) and propylparaben were mixed and heated to 50 to 100 degrees Celsius to prepare Formula # 2. Next, after mixing and emulsifying Formulation # 1 and Formulation # 2 at 2000 to 10000 rpm, the mixture was cooled to 50 degrees Celsius, and additionally cooled to 30 degrees Celsius after adding fragrance to prepare a lotion composition (A).

1-2: Preparation of Vasodilation Lotion Composition (B)

In order to prepare the lotion composition (B) according to the present invention, first, glycerin, xanthan gum, allantoin, dipotassium glycyrrhizate, glycerin-26 (Glycereth-) 26), licorice root extract, raspberry extract, cinnamon extract, honenia dulcis extract, japonica extract, phosphatidylcholine lipoid, retinol, chrysin (chrysin), nitrous acid (sodium salt), aspartic acid, citric acid and methylparaben in aseptic conditions in predetermined proportions in a mixer or mixer. After mixing, the mixture was heated at 50 to 100 degrees Celsius to prepare Formulation # 1. In addition, hydrogenated lecithin, glyceryl stearate SE, PEG-100 stearate, cetearyl alcohol, caprylic acid, capric glyceride ), Mineral oil, grape seed oil (vitis vinifera seed oil), dimethicone (dimethicone), olive oil (olea europaea fruit oil) and propylparaben were mixed and heated to 50 to 100 degrees Celsius to prepare Formula # 2. Next, after mixing and emulsifying Formulation # 1 and Formulation # 2 at 2000 to 10000 rpm, the mixture was cooled to 50 degrees Celsius, and further cooled to 30 degrees Celsius after adding fragrance to prepare a lotion composition (B).

1-3: Preparation of Vasodilatation Gel Composition (A)

The gel composition (A) according to the present invention comprises a xanthan gum, glycerin, sodium nitrite salt, citric acid (extreme), tocopherol, turmeric extract and bamboo extract and adjust the final 100% with distilled water under a sterilizer or mixer. ), The mixture was heated at 50 to 100 ° C, cooled to 50 degrees Celsius after emulsification, and further cooled to 30 degrees Celsius after adding the fragrance.

1-4: Preparation of Vasodilatation Gel Composition (B)

Gel composition (B) according to the present invention, xanthan gum, glycerin, chrysine, retinol, acid type (ascorbic acid, glutamic acid, etc.), sodium nitrite salt (trace amount), citric acid (trace amount), tocopherol, turmeric extract and bamboo extract After mixing 100% with final distilled water and mixed in a stirrer or mixer under sterile drying, heated at 50 ~ 100 ℃, cooled to 50 degrees Celsius after emulsification, and added to the flavor to 30 degrees Celsius Prepared by further cooling.

Example  2: according to the invention Xanthan Gum  Suitable as fixation material for gel

In this example, suitability was evaluated as a trap material of the xanthan gum gel and known control gel according to the present invention. Specifically, Carbopol gels; Cabopol gel + raw material; Trans gels; Trans gel + raw material; Xanthan gum gel; Xanthan gum gel + raw material; KY gel; And KY gel + gel solid state retention of the raw material (%) was observed.

There was no difference in percent gel solids retention between Carbopol gels, Trans gels, xanthan gum gels and KY gels containing no raw materials. However, in the presence of raw materials, Carbopol gel, Trans gel and KY gel showed a sharp drop in solid state retention (%), whereas xanthan gum gel did not change at all (FIG. 1A).

In addition, Carbopol + TEA gel; Carbopol + TEA Gel + Raw Material; Xanthan gum + glycerin; And it was observed whether the xanthan gum + glycerine + raw material gel retention. As expected, Carbopol + TEA gels remain gel solid when no raw materials are present, but the gel structure is rapidly destroyed and liquefied when the raw materials are present, whereas xanthan gum + glycerin changes completely regardless of the presence of raw materials This suggests that the resistance to acid increased due to the presence of xanthan gum (FIG. 1B).

Example  3: vasodilation efficacy of the gel or lotion composition according to the invention

In this example, the vasodilatation efficacy of the gel or lotion composition and the control composition according to the present invention was evaluated. Specifically, 30 minutes after applying the gel composition according to the present invention prepared as shown in Example 1 and the control gel composition to the hands, the degree of peripheral vasodilation was compared.

Details of the subjects who participated in this experiment are as follows:

Experimental group

Gender: 4 males / 5 females,

Age: 20s-2, 30s-4, 40s-1, 50s-2

HEALTH AND SKIN CONDITIONS: Both were healthy and normal.

Control group

Gender: 3 male / 3 female,

Ages: 20s-2, 30s-2, 40s-2

HEALTH AND SKIN CONDITIONS: Both were healthy and normal.

In the subject to which the gel composition according to the present invention was applied, the skin color remained red due to peripheral vasodilation, whereas in the subject to which the control gel composition was applied, the skin color was pale due to vascular recontraction. This means that the gel or lotion composition according to the invention has a higher skin moisturizing and raw material absorption compared to the control gel composition (FIG. 2).

In addition, 5 minutes after each of the gel lotion compositions according to the present invention were applied by 1 g or more, compared to before the gel composition according to the present invention, the skin color of these subjects was changed to a deep scarlet color. Means that the blood vessels expanded after the gel composition according to the invention was applied (FIG. 3).

Example  4: effect of improving blood circulation of gel composition according to the present invention

In this example, changes in blood circulation due to administration of the gel composition according to the present invention were investigated. Specifically, before the application of the gel composition according to the present invention prepared as shown in Example 1 and 5 minutes after application, the skin color and vascular pulse wave measurement device by the expansion of the blood vessels (Fig. 4b) The vascular pulse wave (Pulse Height) change was observed.

Details of the subjects who participated in this experiment are as follows.

Gender: 10 male / 4 female

Ages: 20s-2, 30s-8, 40s-4

HEALTH AND SKIN CONDITIONS: Both were healthy and normal.

Compared to before the gel composition according to the invention was applied, 5 minutes after the gel composition according to the invention was applied, the vascular pulse wave increased 1.4 times in these subjects. This result represents the mean value of the data obtained from these subjects (FIG. 4A).

Example  5: improved blood circulation and vasodilation of the gel composition according to the present invention

In this example, the blood circulation improvement and vasodilation efficacy of the gel composition according to the present invention was evaluated. Specifically, before and after 5 minutes of application of the gel composition according to the present invention prepared as shown in Example 1, blood flow by capillary dilation using a peripheral capillary measurement device (FIG. 5C) An increase was observed.

Details of the subjects who participated in this experiment are as follows:

Gender: 4 males / 6 females,

Ages: 30s-4 people, 40s-2 people, 50s-4 people

Health status and skin conditions: Except for men in their 50s who had hyperlipidemia in their blood due to tobacco and alcohol, the rest of the participants had no health problems, and the skin was also normal.

The shape of the normal capillary and its morphology is shown in Figure 5a. In addition, an increase in blood flow was clearly observed in these subjects 5 minutes after the gel composition according to the present invention was applied, compared to before the gel composition according to the present invention, which is shown in FIG. 5B. . The figure shows an increase in capillary blood flow in men in their fifties who were most serious of vascular problems with hyperlipidemia.

Example  6: improvement of blood circulation and vasodilation of the lotion composition according to the present invention

In this example, the vasodilation efficacy of the lotion composition according to the present invention was evaluated. Specifically, after applying the lotion composition according to the present invention prepared as shown in Example 1 by 1g to the ear and hand by using a thermal imaging camera to compare the heat changes.

In the subject to which the lotion composition according to the present invention was applied, body heat increased after application compared to before application (FIG. 6A). In addition, it was found that the body heat increased even after applying the lotion composition of the present invention to the hands of the hands with cold hands and feet symptoms (Fig. 6b).

Example  7: of the extract contained in the gel or lotion according to the invention cAMP  Produce efficacy

In this example, the cAMP production efficacy of the extract component and the known control extract component included in the gel or lotion according to the present invention was evaluated. Specifically, a control group treated with 1 μM of forskolin, an adenylyl cyclase active substance, in human kidney cells (Human Embryonic Kidney cell), an experimental group treated with turmeric extract 1 mg / ml, and turmeric The amount of cAMP production in the experimental group treated with 1 mg / ml of the mixture of the extract, the bamboo extract and the japonica extract was measured.

Specifically, the HEK cells were stimulated with agonists for 20 minutes in the presence of a phosphodiesterase inhibitor Ro 20-1724 (5 μM), and the reaction was terminated by repeating the freezing and thawing cycle three times. The sample obtained thereafter was obtained by Brown et al. According to the method, centrifuged at 12,000xg for 4 minutes at 4 ℃. The cAMP assay measured competitive binding of ³ H-labeled cAMP and unlabeled cAMP to cAMP-binding proteins isolated from bovine adrenal cortex. Binding ³ H-cAMP present in the supernatant was determined by Liquid Scintillation Counter. Each sample was incubated for 2 hours at 4 ° C. with 50 μl of ³ H-labeled cAMP (5 μCi) and 100 μl of binding protein. Protein-bound cAMP was separated from unbound cAMP by uptake of free cAMP on 100 μl of charcoal and centrifuged at 4 ° C. at 12,000 × g. 200 μl of the supernatant was mixed in an Eppendorf tube containing 1.2 ml scintillation and radioactivity was measured. It was determined using a standard curve of cAMP concentration in the sample and expressed in picomoles per microgram of protein.

As a result, the cAMP production amount was 125% for turmeric extract, the turmeric extract, bamboo extract and japonica extract up to 150% based on 100% cAMP production amount when treated with known control group forskolin. Increased (FIG. 7).

Example  8: promoting hair growth or preventing hair loss of the composition according to the present invention

The inventors examined whether the composition according to the invention activates the K + channel involved in the activity of the hair follicle.

Specifically, as an electrophysiological method, a cell-attached patch configuration was used among patch clamp techniques. Channel activity was measured using a patch-clamp amplifier (Axopatch-1D, Axon Instruments; Foster City, Calif.). 5- to 10-M resistance pipettes were extracted from borosilicate glass capillaries using a vertical puller (model PP-83, Narishige Tokyo, Japan) using a vertical puller (model PP-83, Narishige Tokyo, Japan). The tips of these pipettes were coated with Sylgard and subjected to a slight fire polish. The membrane current was digitized at a sampling rate of 20 kHz and stored in digital format on a digital audiotape using a recorder (model DTR-1200, Biologic; Grenoble, France). To analyze single channel activity, data was transferred to a personal computer with pCLAMP software (version 6.3; Axon Instruments, Union City, CA) via an analog-to-digital conversion interface (Digidata-1200, Axon Instruments). Pinacyl (50 μM) was used as the K + channel agent, and 0.1 mg / ml of a 1: 1 mixture of the compositions A and B of the present invention was used as an agonist, and muscle cells were used.

As a result, as shown in Figure 8 it can be seen that the composition of the present invention to increase the membrane current of the K + channel to activate the K + channel. This is the same mechanism that the composition of the present invention phosphorylates the K + channel by the activity of cAMP and cGMP and the protein kinase G activity, which in turn activates the sulfonelurea receptor. Therefore, it can be seen that the present invention is effective in promoting hair growth or preventing hair loss through activation of the K + channel.

1 shows the differences in solid state retention (FIG. 1A) and gel state (FIG. 1B) of known control gels and gels according to the invention.

Figure 2 shows the active ingredient fixation efficacy at 30 minutes after application of known control gels and gels or lotions according to the invention.

Figure 3 shows a photograph comparing the blood circulation improving efficacy of the known control and the lotion or gel of the present invention.

Figure 4 shows the value of the blood vessel pulse wave (Pulse Height) change (Fig. 4a) and the pulse wave measuring device (Fig. 4b) by the expansion of the skin.

FIG. 5 shows a normal capillary morphology (FIG. 5A), a blood flow increase picture by capillary expansion (FIG. 5B), and a peripheral capillary measurement device (Hin Hi Tech, Korea, USA) (FIG. 5C).

Fig. 6 shows a thermographic change 6a of the ear after application of the lotion according to the present invention, a thermography change 6b of the hand after application of the lotion, and a thermal imaging camera FLIR Therma CAM ^™ , Sweden (Fig. 6c). .

Figure 7 shows a graph comparing the amount of cAMP production induced by the known control extract and the extract according to the present invention.

Figure 8 shows the figure showing the effect of the composition according to the invention on the K + channel membrane current increase and decrease.

Claims (11)

(1) licorice root extract, curcuma longa extract, grape extract, grape seed oil, grapefruit extract, bamboo extract, raspberry extract, cinnamon extract, holly tree ( At least one extract selected from the group consisting of Hovenia dulcis extracts, Japonica extracts, alder extracts, or mixtures thereof; (2) at least one member selected from the group consisting of ammonia, nitrite, nitroglycerline, silver nitrate, magnesium nitrite, ammonium nitrate or mixtures thereof; And (3) malic acid, hyaluronic acid, citric acid, citric acid, vitamin C, tocopherol, aspartic acid, aspartic acid, tartaric acid, succinic acid, retinol Retinol), glutamic acid (glutamic acid), and an acid for additives comprising at least one selected from the group consisting of, the composition for applying the skin having blood circulation improvement and vasodilation. The composition of claim 1, wherein the composition is in the form of a gel or lotion. The composition of claim 2, wherein the composition further comprises xanthan gum or glycerin. 4. Animal and vegetable fats, oils, waxes, paraffins, starches, tragacanths, cellulose derivatives, polyethylene glycols, silicones, bentonites. 1 selected from the group consisting of silicic acid, talc, zinc oxide, Allantoin, Dipotassium Glycyrrhizate, Glycereth-26, Glucose, or mixtures thereof A composition further comprising at least a species. The composition of claim 1 further comprising a transdermal penetration enhancer. The composition of claim 5, wherein the transdermal penetration enhancer comprises at least one selected from lecithin, other phospholipid components used in cosmetics, or liposome components. The method of claim 1, further comprising an adjuvant selected from the group consisting of emulsifiers, surfactants, thickeners, preservatives, antioxidants, sunscreens, colorants, pH adjusters, moisturizers, lubricants, natural flavors, cosmetic flavors, and herbal medicines. A composition comprising a. According to claim 1, wherein the composition improves the symptoms of hair loss due to impaired blood circulation, peripheral vascular dysfunction, cold hands and feet, Raynaud 'phenomenon, erectile dysfunction, decreased female sexuality, or scalp blood circulation disorder A composition, characterized in that. The composition of claim 1, wherein the composition is for promoting hair growth or preventing hair loss. The composition of claim 1, wherein the turmeric extract comprises methylated curcumin derivatives. delete

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