KR20200136737A - Composition for Skin Moisturizing, Improving Skin Wrinkle and Elasticity comprising PQ1 Succinic Acid - Google Patents

Abstract

The present invention provides a cosmetic composition for moisturizing skin, reducing wrinkles or improving elasticity, comprising PQ1 succinic acid as an active ingredient. The present invention provides an effect of promoting the synthesis of collagen, elastic fibers and hyaluronic acid, moisturizing the skin, reducing skin wrinkles and enhancing elasticity.

Description

Composition for Skin Moisturizing, Improving Skin Wrinkle and Elasticity comprising PQ1 Succinic Acid}

The present invention relates to a cosmetic composition for moisturizing skin, improving wrinkles and improving elasticity, comprising PQ1 succinic acid as an active ingredient.

Skin wrinkles are known to be caused by a decrease in collagen and elastic fibers, which are major components of the extracellular matrix. Collagen is a major matrix protein produced by skin fibroblasts, and accounts for about 30% of the total weight of biological proteins. The main functions of collagen are known for its mechanical firmness, connective tissue resistance and tissue bonding, support for cell adhesion, and induction of cell division and differentiation (when organisms grow or heal wounds). In addition, collagen is a matrix protein that plays an important role in retaining moisture in the dermal layer. These matrix proteins adsorb moisture and increase moisture retention in the structures formed by them, allowing the skin to maintain a state containing adequate moisture. It is known to be involved in maintaining the elasticity of the skin. Elastic fibers account for 3% of the dry weight of the skin and provide elasticity to the skin. Elastic fibers can be classified into three types: oxytalan, elanin, and elastic fiber, and the type is determined by the combination of fibrillin and elastin. These collagens and elastic fibers are reduced by age and photoaging caused by ultraviolet irradiation, which is known to be closely related to the formation of wrinkles in the skin. In order to solve this problem, interest in substances that promote collagen and elastic fiber synthesis has increased, and known substances that promote collagen synthesis include vitamin C, retinoic acid, retinol, protein derived from animal placenta, There are various plant extracts such as fucoidan and chlorella. However, when these substances are applied to the skin, the amount of use of these substances is limited due to safety problems such as irritation and redness, or the effect is insignificant, and thus the effect of improving skin wrinkles could not be expected. Accordingly, there is an urgent need to develop a new anti-wrinkle and improved skin external composition composition that is safer and more effective than the existing external composition for skin.

On the other hand, the skin barrier not only protects against various stimuli from the outside, but also protects against excessive dissipation of moisture in the body through the skin.This protective function can only be maintained when the stratum corneum composed of keratinocytes is normally formed. I can. The stratum corneum of the epidermis contains 10% to 20% of moisture and is present on the outermost part of the human body, thereby inhibiting evaporation of water from the body while preventing excess moisture from penetrating from the outside. However, when the moisture content of the stratum corneum is less than 10%, the skin becomes rough and the body's protective function is lost. Epidermal keratinocytes produce an important matrix that traps moisture in the epidermal layer called hyaluronic acid. It is known that the amount of hyaluronic acid decreases with aging, and the decrease in the amount of hyaluronic acid in the skin with age is believed to be one of the direct causes of wrinkles and moisture content reduction in the skin. Three types of hyaluronic acid synthase known so far have been reported: hyaluronan synthase-1 (HAS-1), hyaluronan synthase-2 (HAS-2), and hyaluronan synthase-3 (HAS-3). There has been a lot of research on substances for skin moisturizing, and as the skin moisturizers known so far, humectants or NMF ingredients that absorb moisture, or natural water-soluble ingredients containing these ingredients were used. Rather than fundamentally promoting the synthesis of moisturizing ingredients, the efficacy is limited in the form of external supply. Therefore, there is a need to develop a safe moisturizer that promotes the generation of actual moisturizing factors in dry skin.

Under this background, the present inventors have made intensive research efforts on a substance capable of simultaneously solving skin wrinkles and moisturizing, and as a result, it was confirmed that PQ1 Succinic Acid exhibits an effect of promoting the synthesis of collagen, elastic fibers and hyaluronic acid. By confirming that it has a moisturizing, wrinkle improvement or elasticity enhancing effect, the present invention was completed.

Korean Patent Registration 10-1086504

One object of the present invention is to provide a cosmetic composition for moisturizing skin, improving wrinkles or improving elasticity, comprising PQ1 succinic acid as an active ingredient.

Another object of the present invention is to provide a pharmaceutical composition for skin moisturizing, wrinkle improvement or elasticity enhancement comprising PQ1 succinic acid as an active ingredient.

Another object of the present invention is to provide a quasi-drug composition for skin moisturizing, wrinkle improvement or elasticity enhancement comprising PQ1 succinic acid as an active ingredient.

This will be described in detail as follows. Meanwhile, each description and embodiment disclosed in the present invention can be applied to each other description and embodiment. That is, all combinations of various elements disclosed in the present invention belong to the scope of the present invention. In addition, it cannot be seen that the scope of the present invention is limited by the specific description described below.

As an aspect for achieving the above object, the present invention provides a cosmetic composition for moisturizing skin, improving wrinkles or improving elasticity, comprising PQ1 succinic acid as an active ingredient.

The PQ1 succinic acid may be represented by Formula 1 below.

[Formula 1]

In addition, PQ1 succinic acid has the chemical name N1-[6-methoxy-4-methyl-5-[3-(trifluoromethyl)phenoxy]-8-quinolinyl]-1,3-propanediamine butanedioic acid (C ₂₅ H ₂₈ F ₃ N ₃ O ₆ , molecular weight: 523.50 g/mol).

The PQ1 succinic acid is a material known as a gap junction enhancer. Gap junction promoters are water, ions less than 1200Da, low-molecular substances (Ca ²⁺ , IP3, nutrients, metabolites), glutamate, and the passage of electrical signals. , It is known to regulate differentiation and death.

These functional abnormalities of gap junctions have been observed in neurological diseases such as Parkinson's disease, arrhythmia, and diseases such as cancer, and a gap junction enhancer has become a target for the development of therapeutic agents to treat these diseases.

It has been reported that PQ1 succinic acid increases the gap junction activity in breast cancer cells and, when combined with an anticancer drug, increases the therapeutic effect of an anticancer drug, and is effective in the treatment of ischemia and parasitic infections, but there is no known about skin improvement. The present invention has come to complete a composition for improving skin wrinkles and skin using PQ1 succinic acid.

The cosmetic composition according to the present invention is a solution, external ointment, cream, foam, nutrient lotion, soft lotion, pack, softening water, emulsion, makeup base, essence, soap, liquid cleaning agent, bathing agent, sunscreen cream, sun oil, suspension, Emulsion, paste, gel, lotion, powder, soap, surfactant-containing cleansing, oil, powder foundation, emulsion foundation, wax foundation, can be prepared in a formulation selected from the group consisting of a patch and spray, but limited thereto. It is not.

The cosmetic composition may be characterized by moisturizing the skin, improving wrinkles and improving elasticity.

The "wrinkles" can be largely classified into facial wrinkles caused by facial expression muscles used to make facial expressions and fine wrinkles caused by overall or local skin weakness. Specifically, wrinkles may be caused by genes, reduction of collagen and elastic fibers present in the skin dermis, and external environment. For the purposes of the present invention, wrinkles may be used in the sense of including all facial wrinkles or fine wrinkles, but are not limited thereto. Meanwhile, the term "moisturizing" as used in the present invention refers to any action that maintains the smooth surface by supplying moisture to the skin or blocking the evaporation of moisture to maintain the skin's flexibility and induce uniform exfoliation of dead skin cells, “Dry” refers to a condition in which such skin moisturization is insufficient.

Specifically, the PQ1 succinic acid may provide a cosmetic composition characterized in that promoting collagen synthesis.

According to an embodiment of the present invention, it was confirmed that PQ1 succinic acid has the effect of increasing the total amount of collagen by promoting collagen synthesis (Table 1 and FIG. 1).

Through this, it was found that the composition containing PQ1 succinic acid is effective in moisturizing the skin, improving wrinkles, and enhancing elasticity.

Specifically, the PQ1 succinic acid can provide a cosmetic composition, characterized in that promoting the synthesis of elastic fibers.

According to an embodiment of the present invention, it was confirmed that PQ1 succinic acid has an effect of increasing the total amount of fibrillin by promoting fibrillin synthesis (Tables 2 and 2).

Specifically, the PQ1 succinic acid can provide a cosmetic composition, characterized in that promoting the synthesis of hyaluronic acid.

According to an embodiment of the present invention, it was confirmed that PQ1 succinic acid has an effect of increasing the synthesis of hyaluronic acid synthase (Tables 3 to 4 and FIGS. 3 to 4).

The HAS2 and HAS3 used in Tables 3 to 4 and FIGS. 3 to 4 will be described as follows. Hyaluronic acid (Hyaluronan, HA) is a major component of skin and extracellular matrix. It is well known for promoting the movement of nutrients and ions and retaining moisture as a major component of the extracellular matrix. In addition, it has been reported that more than 50% of HA present in the mammalian body is distributed in the skin, especially the intercellular spaces of the epidermis and the connective tissues of the dermis, which is involved in maintaining the intercellular space, division and differentiation of cells, migration, and immune regulation. , These HAs are known to be mainly synthesized by epidermal keratinocytes and dermal fibroblasts.

It has been reported that the amount of HA in human skin decreases with aging, and is considered to be one of the direct causes of the decrease in skin elasticity and moisture content. It has been reported that the synthesis of HA in the skin cell culture state is increased by various types of growth factors. It is known that the synthesis of HA proceeds by Hyaluronan synthases (HAS) on the inner surface of the cell membrane, and during the synthesis, it penetrates the cell membrane and accumulates in the extracellular matrix. Three types of HAS genes have been reported: Hyaluronan synthase-1 (HAS1), Hyaluronan synthase-2 (HAS2), and Hyaluronoan synthase-3 (HAS3) with high nucleotide sequence similarities.

Accordingly, the present invention increased the expression of HAS2 and HAS3 mRNA in epidermal cells by PQ1 succinic acid, which leads to an increase in the production of hyaluronic acid, confirming the effects of moisturizing, wrinkle improvement, and elasticity enhancement.

As used herein, the term "improvement" means to alleviate the symptoms of wrinkles and dryness of the skin by using the composition of the present invention, and "prevention" means preventing the generation of wrinkles and dry skin symptoms, or It includes anything that delays the occurrence of dry skin.

Specifically, the cosmetic composition further comprises one or more substances selected from the group consisting of oil, water, surfactant, moisturizer, alcohol, thickener, chelating agent, colorant, preservative and fragrance, to provide a cosmetic composition. However, it is not limited thereto.

It may vary depending on the cosmetically acceptable formulation contained in the cosmetic composition of the present invention.

When the formulation of the present invention is an ointment, paste, cream or gel, animal oil, vegetable oil, wax, paraffin, starch, tracant, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc, zinc oxide, or mixtures thereof Can be used.

In addition, when the formulation of the present invention is a powder or spray, lactose, talc, silica, aluminum hydroxide, calcium silicate, polyamide powder, or a mixture thereof may be used. In particular, in the case of a spray, additional chlorofluoro Propellants such as rohydrocarbon, propane/butane or dimethyl ether.

In addition, when the formulation of the present invention is a solution or emulsion, a solvent, a solubilizing agent or an emulsifying agent may be used, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1 ,3-butylglycol oil may be used, in particular cottonseed oil, peanut oil, corn germ oil, olive oil, castor oil and sesame oil, glycerol aliphatic ester, polyethylene glycol or fatty acid ester of sorbitan may be used. .

In addition, when the formulation of the present invention is a suspension, liquid diluents such as water, ethanol or propylene glycol, ethoxylated isostearyl alcohol, suspending agents such as polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, crystallites Sex cellulose, aluminum metahydroxide, bentonite, agar or tracant, and the like can be used.

In addition, when the formulation of the present invention is a soap, an alkali metal salt of a fatty acid, a fatty acid hemiester salt, a fatty acid protein hydrolyzate, isethionate, a lanolin derivative, an aliphatic alcohol, vegetable fat, glycerol, sugar, etc. can be used. have.

The cosmetic composition of the present invention is an auxiliary agent commonly used in the sitting fan extract or its fractions, such as hydrophilic or lipophilic gelling agents, hydrophilic or lipophilic activators, preservatives, antioxidants, solvents, fragrances, fillers, blockers, pigments, odorants. And dyes and the like.

Specifically, the PQ1 succinic acid may provide a cosmetic composition that is 0.0001% by weight to 10% by weight based on the total weight of the composition.

If the PQ1 succinic acid is less than 0.0001% by weight of the total composition weight, it may not be desirable because the original target activity cannot be sufficiently achieved, and if it exceeds 10% by weight, a distinct effect is not expected to increase as much as the increasing content. It can be uneconomical.

As another aspect for achieving the above object, the present invention provides a pharmaceutical composition for skin moisturizing, wrinkle improvement or elasticity enhancement comprising PQ1 succinic acid as an active ingredient.

The pharmaceutical composition of the present invention may be used as a single formulation, and may be prepared and used as a combined formulation, further including drugs known to have an effect of improving skin moisturization, wrinkle improvement and elasticity enhancement, and a pharmaceutically acceptable carrier or excipient It can be prepared in a unit dosage form by using and formulated into a unit dosage form, or can be prepared by placing it in a multi-dose container.

The pharmaceutical composition may be administered in a pharmaceutically effective amount.

The "pharmaceutically effective amount" means an amount sufficient to treat a disease with a reasonable benefit/risk ratio applicable to medical treatment, and the effective dose level is the type and severity of the subject, age, sex, activity of the drug, and Sensitivity to, time of administration, route of administration and rate of excretion, duration of treatment, factors including drugs used concurrently, and other factors well known in the medical field.

As another aspect for achieving the above object, it provides a quasi-drug composition for skin moisturizing, wrinkle improvement or elasticity enhancement comprising PQ1 succinic acid as an active ingredient.

In the present invention, the quasi-drug composition may have skin moisturization, wrinkle improvement, and elasticity enhancement effects, but is not limited thereto.

The quasi-drug composition of the present invention may further include a pharmaceutically acceptable carrier, excipient, or diluent as needed in addition to the above ingredients. The pharmaceutically acceptable carrier, excipient, or diluent is not limited as long as it does not impair the effects of the present invention, for example, fillers, extenders, binders, wetting agents, disintegrants, surfactants, lubricants, sweeteners, fragrances, preservatives, etc. Can include.

The "pharmaceutically acceptable carrier" may mean a carrier, excipient, or diluent that does not irritate an organism and does not inhibit the biological activity and properties of the compound to be injected, and specifically, a non-naturally occuring carrier). The kind of the carrier that can be used in the present invention is not particularly limited, and any carrier commonly used in the art and pharmaceutically acceptable can be used. Non-limiting examples of the carrier include saline, sterile water, Ringer's solution, buffered saline, albumin injection solution, dextrose solution, maltodextrin solution, glycerol, ethanol, and the like. These may be used alone or in combination of two or more.

The composition including a pharmaceutically acceptable carrier may be in various oral or parenteral formulations. In the case of formulation, it is prepared using diluents or excipients such as fillers, extenders, binders, wetting agents, disintegrants, and surfactants that are usually used. Specifically, solid preparations for oral administration include tablets, pills, powders, granules, capsules, and the like, and these solid preparations include at least one excipient, for example, starch, calcium carbonate, sucrose, lactose, It can be prepared by mixing gelatin or the like. Further, in addition to simple excipients, lubricants such as magnesium stearate and talc may also be used. Liquid preparations for oral use include suspensions, liquid solutions, emulsions, syrups, and other excipients, such as wetting agents, sweeteners, fragrances, and preservatives, in addition to water and liquid paraffin, which are commonly used simple diluents. have. Formulations for parenteral administration include sterile aqueous solutions, non-aqueous solutions, suspensions, emulsions, lyophilized formulations and suppositories. As the non-aqueous solvent and suspending agent, propylene glycol, polyethylene glycol, vegetable oil such as olive oil, injectable ester such as ethyl oleate, and the like can be used. As a base for suppositories, Witepsol, Macrogol, Tween 61, cacao butter, laurin paper, glycerogelatin, and the like can be used.

The quasi-drug composition of the present invention may exemplify a disinfectant cleaner, a shower foam, an ointment, a wet tissue, a coating agent, etc., but is not limited thereto, and the formulation method, dosage, usage method, component, etc. of quasi-drugs are known in the art. It can be appropriately selected from the techniques of.

In addition, it can be used in a method for improving skin wrinkles and dry skin, comprising administering a quasi-drug composition comprising the PQ1 succinic acid as an active ingredient of the present invention to individuals other than humans. The individual may include, without limitation, mammals, including mice, livestock, and humans.

The composition comprising PQ1 succinic acid of the present invention provides a method for moisturizing skin, improving wrinkles or enhancing elasticity, comprising administering to a subject.

The terms "PQ1 succinic acid", "wrinkle", "moisturizing", "dry", "prevention" and "improvement" used in the present invention are as described above.

The term "administration" used in the present invention means introducing the composition of the present invention to a patient by any suitable method, and the route of administration of the composition may be administered through any general route as long as it can reach the target tissue. . Intraperitoneal administration, intravenous administration, intramuscular administration, subcutaneous administration, intradermal administration, oral administration, topical administration, intranasal administration, but are not limited thereto. Since the composition of the present invention is effective in improving skin wrinkles, the route of administration of the composition may be applied to the skin and administered.

The composition of the present invention may be administered daily or intermittently, and the number of administrations per day may be administered once or divided into 2-3 times. In addition, the composition of the present invention may be used alone or in combination with other drug treatments to improve skin wrinkles. It is important to administer an amount capable of obtaining the maximum effect in a minimum amount without side effects in consideration of all the above factors, and can be easily determined by a person skilled in the art.

In the present invention, the term "individual" refers to all animals such as rats, mice, and domestic animals, including humans, where wrinkles or skin wrinkles have occurred or may occur. Specifically, it may be a mammal including a human.

Wrinkle improvement, skin moisturizing and elasticity enhancing composition comprising PQ1 succinic acid according to the present invention as an active ingredient provides an increasing effect of collagen, elastic fiber and hyaluronic acid synthase, and provides skin moisturizing, wrinkle improvement and elasticity enhancing effect.

1 is a graph showing the effect of promoting collagen synthesis according to the concentration of PQ1 succinic acid.
2 is a graph showing the effect of accelerating fibrillin synthesis according to the concentration of PQ1 succinic acid.
3 is a graph showing the effect of promoting hyaluronic acid synthase 2 (HAS2) production according to the concentration of PQ1 succinic acid.
4 is a graph showing the effect of promoting hyaluronic acid synthase 3 (HAS3) production according to the concentration of PQ1 succinic acid.

Hereinafter, the present invention will be described in more detail through examples. These examples are for explaining the present invention more specifically, and the scope of the present invention is not limited by these examples.

Example 1: Test for increasing the expression of collagen and fibrillin in human dermal fibroblasts

In order to confirm the effect of increasing the expression of collagen and fibrillin of PQ1 succinic acid, it was attempted to confirm the expression levels of collagen and fibrillin mRNA. The specific experimental method is as follows.

Human dermal fibroblats are dispensed into a cell culture plate (6-well cell culture plate) each 4x10 ⁵ for 24 hours in an animal cell culture medium (DMEM) containing 10% fetal bovin serum. Cultured. After replacing the cultured cells with an animal cell culture medium (DMEM) that does not contain 10% bovine serum, PQ1 succinic acid was diluted in the medium and treated for 24 hours. After 24 hours, the cells were recovered, washed with cooled phosphate buffer solution (PBS), and total RNA was extracted using an RNeasy mini kit (Qiagen, Germany). Reverse transcription reaction was performed for RNA using cDNA Synthesis Kit (PhileKorea, Korea). The synthesized cDNA was quantified and then diluted with water, and the same amount was used for all reactions. TaqMan® Universal Master Mix II and TaqMan® Gene Expression Assays (Thermo Fisher, USA) collagen type 1 (COL1) and fibrillin (FBN) primers were used. Real-time polymerase chain reaction (quantitative real time PCR) was performed using the StepOnePlus® Real-time PCR System (Applied Biosystems, USA).

Experimental results are shown by calculating the ΔΔCt method based on the housekeeping gene Glyceraldehyde-3-phosphate dehydrogenase (GAPDH). The expression levels of the COL1 and FBN genes of each sample were numerically calculated based on the mRNA expression level of the negative control group to which the extract was not added to about 1.0, and the results are shown in FIGS. 1 to 2 and Tables 1 to 2. TGFb 10ng/ml (Peptrone, Korea) was used as a positive control, and PQ1 succinic acid was tested by dividing into the experimental groups of 100νM and 200νM.

Table 1 and FIG. 1 are tables comparing the expression levels of collagen mRNA.

sample Collagen (COL1) mRNA increase amount Untreated control 1.00 Positive control (TGFb 10ng/ml) 2.57 PQ1 succinic acid 100νM 1.79 PQ1 succinic acid 200νM 3.24

Table 2 and Figure 2 is a table comparing the expression levels of fibrilin mRNA.

sample Fibrillin (FBN) mRNA increase Untreated control 1.00 Positive control (TGFb 10ng/ml) 2.91 PQ1 succinic acid 100νM 2.13 PQ1 succinic acid 200νM 4.60

Taken together, the above results showed that the experimental group treated with PQ1 succinic acid showed increased collagen and fibrillin mRNA increase compared to the negative control (untreated control), and in particular, the experimental group treated with PQ1 succinic acid 200νM was compared with the positive control. Even so, it was found that the expression level was significantly increased.

Through these results, it was confirmed that the PQ1 succinic acid of the present invention has effects such as skin moisturization, wrinkle improvement, and elasticity enhancement by increasing the expression levels of collagen and fibril (FIGS. 1 to 2).

Example 2: HAS (hyaluronan synthase) 2 and HAS (hyaluronan synthase) 3 expression increase test in human keratinocytes

Next, it was attempted to confirm the effect of increasing the expression of hyaluronic acid synthase of PQ1 succinic acid. The increase in hyaluronic acid synthase (HAS) leads to an increase in the production of hyaluronic acid (HA), which can have an effect on moisturizing, wrinkles, and elasticity. HAS2 and HAS3 were used as hyaluronic acid synthase.

The HAS2 and HAS3 are synthases that are located on the inner surface of the cell membrane to synthesize hyaluronic acid. The increase in the mRNA of HAS2 and HAS3 leads to the production of hyaluronic acid, which is a major component of the skin and extracellular matrix, so as to moisturize, improve wrinkles, and improve elasticity. You can see the effect.

Human keratinocytes (HaCaT) were dispensed into a cell culture plate (6-well cell culture plate) by 2x10 ⁵ each, and cultured in DMEM medium containing 10% fetal bovin serum for 24 hours. After replacing the cultured cells with an animal cell culture medium (DMEM) that does not contain 10% bovine serum and calcium (Ca ²⁺ ), PQ1 succinic acid (Sigma, USA) was diluted in the medium to 24 Time to process. After 24 hours, the cells were recovered, washed with cooled phosphate buffer (PBS), and total RNA was extracted using an RNeasy mini kit (Qiagen, Germany). Reverse transcription reaction was performed using RNA using cDNA Synthesis Kit (PhileKorea, Korea). The synthesized cDNA was quantified and then diluted with water, and the same amount was used for all reactions. TaqMan® Universal Master Mix II and TaqMan® Gene Expression Assays (Thermo Fisher, USA) HAS2, HAS3 primers were used. Real-time polymerase chain reaction (quantitative real time PCR) was performed using the StepOnePlus® Real-time PCR System (Applied Biosystems, USA).

Experimental results are shown by calculating the ΔΔCt method based on the housekeeping gene Glyceraldehyde-3-phosphate dehydrogenase (GAPDH). The expression levels of HAS2 and HAS3 genes of each sample were numerically calculated based on the mRNA expression level of the negative control without the addition of the extract to about 1.0, and the results are shown in FIGS. 3 to 4 and Tables 3 to 4 below. Ca ²⁺ 1.8mM was used as a positive control.

Table 3 and Figure 3 is a table comparing the expression level of HAS2 mRNA.

sample HAS2 mRNA increase Untreated control 1.00 Positive control (Ca ^{2 +} 1.8mM control) 2.70 PQ1 succinic acid 100νM 2.37 PQ1 succinic acid 200νM 3.83

Table 4 and Figure 4 is a table comparing the expression level of HAS3 mRNA.

sample HAS3 mRNA increase Untreated control 1.00 Positive control (Ca ²⁺ 1.8mM) 2.50 PQ1 succinic acid 100νM 1.87 PQ1 succinic acid 200νM 2.95

Taken together, the above results showed that the experimental group treated with PQ1 succinic acid showed increased HAS2 and HAS3 mRNA increase compared to the negative control.In particular, the experimental group treated with PQ1 succinic acid 200νM significantly increased expression compared to the positive control group. It could be seen that the amount was shown.

Through these results, it was confirmed that the PQ1 succinic acid of the present invention increased the expression of hyaluronic acid synthase to promote hyaluronic acid synthesis, thereby having effects such as skin moisturization, wrinkle improvement, and elasticity enhancement (FIGS. 3 to 4). .

Preparation Example 1: Preparation of a cosmetic containing 0.1% of PQ1 succinic acid

Using the ingredients and contents shown in Table 5 below, cream-formed cosmetics of the experimental group containing PQ1 succinic acid and the control group not containing PQ1 succinic acid were prepared (unit: wt%).

ingredient Experimental group Control PQ1 succinic acid 0.1 0 Purified water Balance Balance glycerin 8.0 8.0 Butylene glycol 4.0 4.0 Hyaluronic acid extract 5.0 5.0 Beta glucan 7.0 7.0 Carbomer 0.15 0.15 Caprylic/Capric Triglyceride 8.0 8.0 Squalane 5.0 5.0 Cetearyl glucoside 1.5 1.5 Sorbitan Stearate 0.4 0.4 Cetearyl alcohol 2.0 2.0 antiseptic Appropriate amount Appropriate amount Pigment Appropriate amount Appropriate amount Triethanolamine 0.15 0.15

Experimental Example 1: Changes in skin moisture content before and after application of a cosmetic containing PQ1 succinic acid

In order to confirm the skin moisturizing effect of the cosmetic composition of the present invention, it was attempted to check the rate of change of moisture content after 2 weeks by dividing into an experimental group in which a cosmetic containing PQ1 succinic acid was applied to the skin and a control group applied with a cosmetic composition not containing PQ1 succinic acid. . The specific experimental method is as follows.

For the evaluation, 20 healthy women aged 20 to 30 were selected, and a cosmetic containing PQ1 succinic acid was distributed to the control group and a cosmetics containing no PQ1 succinic acid was distributed to the control group, and the experimental group and the control group were used on the left and right cheeks, respectively. After 2 weeks, the skin moisture content was measured. Specifically, after washing the face of the 20 subjects with soap, the skin was adapted under constant temperature and humidity conditions (temperature 20±2°C, relative humidity 40±2%). Using a Corneometer (CM825), the skin surface electric capacity was repeatedly measured three times.

Table 6 below is a table comparing the rate of change in skin moisture content.

Moisture content change rate after 2 weeks (%) Experimental group 31.4 Control 9.3

In summary, it was confirmed that the experimental group using the cosmetic composition containing PQ1 succinic acid significantly improved and increased the moisture content of the skin than the control group using the cosmetic composition containing no PQ1 succinic acid.

Through these results, it was confirmed that the cosmetic composition including PQ1 succinic acid of the present invention is effective in improving wrinkles and moisturizing the skin by increasing the moisture content of the skin.

In summary, it was confirmed that the cosmetic composition containing PQ1 succinic acid of the present invention promotes the synthesis of collagen, elastic fibers and hyaluronic acid to moisturize the skin, improve wrinkles and improve elasticity.

From the above description, those skilled in the art to which the present invention pertains will understand that the present invention can be implemented in other specific forms without changing the technical spirit or essential features thereof. In this regard, it should be understood that the embodiments described above are illustrative in all respects and not limiting. The scope of the present invention should be construed that all changes or modifications derived from the meaning and scope of the claims to be described later rather than the above detailed description and equivalent concepts are included in the scope of the present invention.

Claims (9)

A cosmetic composition containing PQ1 succinic acid as an active ingredient for moisturizing skin, improving wrinkles or improving elasticity.
The method of claim 1,
The PQ1 succinic acid is 0.0001% to 10% by weight based on the total weight of the composition, the cosmetic composition.
The method of claim 1,
The PQ1 succinic acid is to promote collagen synthesis, cosmetic composition.
The method of claim 1,
The PQ1 succinic acid is to promote the synthesis of elastic fibers, cosmetic composition.
The method of claim 1,
The PQ1 succinic acid is to promote the synthesis of hyaluronic acid, cosmetic composition.
The method of claim 1,
The cosmetic composition further comprises one or more selected from the group consisting of oil, water, surfactant, moisturizer, alcohol, thickener, chelating agent, colorant, preservative and fragrance.
A pharmaceutical composition for skin moisturizing, wrinkle improvement or elasticity enhancement comprising PQ1 succinic acid as an active ingredient.
A quasi-drug composition for skin moisturizing, wrinkle improvement or elasticity enhancement comprising PQ1 succinic acid as an active ingredient.
Skin moisturizing, wrinkle improvement or elasticity enhancement method comprising the step of administering a composition comprising PQ1 succinic acid as an active ingredient to an individual other than humans.

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