KR101072897B1 - COMPOSITION FOR ALLEVIATING A HANGOVER COMPRISING AN EXTRACT OF Trapa japonica Flerov - Google Patents

Abstract

The present invention relates to a hangover relief composition containing a crude herbal extract extract, the composition for hangover elimination of the present invention containing an extract of dried, soft, and strip or as an active ingredient And it promotes the activity of ALDH and has the effect of promoting the decomposition of alcohol and acetaldehyde, so can be useful as a hangover food, beverage.

Dried, lotus root, earthly, brownish, hangover

Description

Hangover Relief Composition Containing Dried Extracts {COMPOSITION FOR ALLEVIATING A HANGOVER COMPRISING AN EXTRACT OF Trapa japonica Flerov}

The present invention relates to a hangover relief composition containing as an active ingredient extracts of dried, soft, and earth or dried, soft, earth, and brown.

Many people are interested in drugs that can get rid of hangovers because of heavy drinking and frequent drinking. Hangovers are often caused by headaches or heartburn, which are caused by alcohol being converted to acetaldehyde, which is harmful to the body during metabolic processes, which is harmful to the digestive system, including the brain and liver.

Unlike other foods, alcohol that can't be stored in tissues is oxidized to acetaldehyde by the catalytic action of alcohol dehydrogenase (ADH), and then to acetic acid and hydrogen by the catalytic action of aldehyde dehydrogenase (ALDH). (Jae-Yeol Cho et al., Korean Food Science Society, 29 (1), 167-172, 1997). 90% of the acetic acid formed is directly CO ₂ and H ₂ O and about 10% is completely degraded via the TCA cycle (Cho Jae-Hyeol et al . ; Lieber CS et al. , Grune amd Stration , 253, 1986).

In addition, acetaldehyde, known as a cause of hangover, has a high affinity with sulfur-containing substances such as cysteine and glutathione in the liver microsomes, and is known to cause liver damage such as fatty liver and liver necrosis by inducing lipid changes in the plasma membrane. (Kaufman N. et al. , AM.A. Arch.Path , 70, 331, 1960).

Alcohol affects the body depending on the amount of intake.In a short time, excessive alcohol intake causes vomiting, headache, lowering blood pressure, tachycardia and shock, along with accumulation of aldehydes, especially in liver lipid oxidation or endoplasmic reticulum. It impedes metabolism, and chronic adaptation leads to adaptation, which increases the metabolism of alcohol and drugs and accelerates the production of lipoproteins, resulting in damage to liver cells (Rosser BG et al. , Gastroenterology , 108, 252, 1995). These harmful phenomena are caused by the destruction of hepatocytes by excessive NADH formed by the peroxidation reaction occurring during the production of acetaldehyde and the catalyst of MEOS (Microsomal Ethanol Oxidizing System), the chemical equilibrium of hepatocytes, metabolic abnormalities and the inhibition of mitochondrial function. (Bunsel RG et al. , Behav. Vrain Res. 1, 351, 1980). These harmful reactions and harmful substances cause hepatic cell damage, leading to the accumulation of fatigue substances such as lactic acid, reducing the activity of ALDH, inhibiting the activation of vitamins, reducing the amount of vitamins in the blood and inhibiting the synthesis of muscle protein in the heart. do. Moreover, increased hydrogen is directly or indirectly involved in the synthesis of fatty acids to form fats and the like, resulting in the pathological phenomenon of fatty liver (Lieber CS et al. , Nutr. Sci. Viaminol. , 29, 509, 1983).

On the other hand, research and experiments on a number of substances that can reduce the toxicity of alcohol or inhibit the expression of toxicity are in progress, and as a result, various health supplements containing ingredients or artificial ingredients extracted from natural foods or herbal ingredients Food is being developed in this regard.

However, in order to solve the harmful effects caused by the hangover or alcohol toxicity caused by drinking, a large number of drinks manufactured using herbal or artificial preparations have been developed and sold. However, there are still no satisfactory effects. to be.

Trapa japonica Flerov is an annual herb belonging to the Hydrocaryaceae, which grows in water. About 15% of the walnut fruit, which is a dried fruit, is composed of starch, and also contains components such as glucose, protein, vitamin C, and vitamin B, and has a slightly sweet taste.

The lotus root is a seed removed from the mature fruit shell of the lotus ( Nelumbo nucifera Gaertn.), Which has a green core inside, has no smell, and tastes slightly sweet. The components of lotus root meat are large amounts of starch and protein, fat, carbohydrates, calcium, phosphorus, iron, etc., and it is excellent for cultivating planting, protecting kidneys and spleen, and converging the intestines. It also has a good effect on macrophages.

Earthling refers to the fruit or seed of the fruit of the buckthorn of Hovenia dulcis Thunb., Which is a flat round, slightly smelly and tastes bitter. Earthlings are used for fever, dry mouth, hiccups, vomiting, etc. to help diuresis and to cure soy liver with alcoholism.

Browning refers to the flowers of Pueraria lobata (Willd.) Ohwi, which controls headaches, fever, thirst, anorexia, bloating and vomiting caused by heavy drinking.

Accordingly, the present inventors have completed the present invention by confirming that the composition containing the extract of dried, soft, and earth or the extract of dried, soft, earth, and browning as an active ingredient has an excellent effect on the hangover.

SUMMARY OF THE INVENTION An object of the present invention is to provide a hangover herb composition for promoting hangover activity of ADH and ALDH, which has an effect of promoting decomposition of alcohol and acetaldehyde.

In accordance with the above object, the present invention provides a hangover relief composition containing the extract of dried, soft and the earth as an active ingredient.

In another aspect, the present invention provides a hangover relief composition containing the extract of dried, lotus root, earth and browning as an active ingredient.

Hangover-relieving composition of the present invention containing the extract of dried, softened and stripped or dried, softened, stripped and browned as an active ingredient to promote the decomposition of alcohol and acetaldehyde by promoting the activity of ADH and ALDH It can be usefully used as a food and beverage for hangover.

Hangover composition of the present invention is an active ingredient of the extract of dried, soft, and earth or the extract of dried, soft, earth, and browning of 0.01 to 95% by weight, preferably 1 to 80% by weight of the total composition Include as.

In addition, the hangover relief composition of the present invention extracts the raw material before extraction of dried, soft meat and earth strips in a weight ratio of 1.0 to 8.0: 0.75 to 3.0: 1, preferably 2.0 to 7.0: 1.0 to 2.0: 1.

In addition, the hangover relief composition of the present invention is a weight ratio of 1.3 ~ 16.0: 0.7 ~ 6.0: 0.6 ~ 4.0: 1, preferably 2.0 ~ 8.0: 1.0 ~ 3.0: Extract at a weight ratio of 1.0 to 2.0: 1. Preferred weight ratios may vary depending on the condition of the raw materials.

In addition, it is preferable that the dryness of the hangover relief composition of this invention contains an outer shell.

In addition, the hangover composition of the present invention may further include extracts of other herbal medicine known in the art that is effective in relieving hangover.

The herbal extract contained in the hangover relief composition of the present invention may be prepared by extracting the herbal medicine itself as it is or in the form of a fine or powdered form, using a conventional extraction method, for example, hot water extraction or an organic solvent. In addition, the hangover composition of the present invention may comprise a mixture of a dry powder or a commercial herbal extract powder obtained by drying the extract as a herbal extract. At the time of extract preparation, the herbal medicines may be dried, not dried, or mixed with each other.

The herbal extracts contained in the hangover relief composition of the present invention may be, for example, chopped dried herbal medicines, and then stored at room temperature to 60 to 130 ° C. with water, an organic solvent or a mixed solvent of about 3 to 1000 times the weight. After extracting repeatedly 1 to 5 times by the extraction method such as hot water extraction, cold sedimentation, ultrahigh pressure extraction or reflux cooling extraction for about 1 hour to 10 days at the extraction temperature of the extract, the extract is filtered, concentrated under reduced pressure or lyophilized Can be. Examples of the organic solvent may include C _1-4 lower alcohol, ethyl acetate, chloroform, hexane, dichloromethane, and the like.

The herbal extract of the present invention, as confirmed in the test example of the present invention, exhibits the effect of promoting ADH activity and promoting ALDH activity, and shows the effect of promoting alcohol and acetaldehyde degradation in an in vivo experiment using rats. Therefore, the hangover relief composition of the present invention containing the herbal extract as an active ingredient shows an excellent hangover relief effect.

The present invention also provides a hangover relief health promoting food containing the herbal extract as an active ingredient.

The health promoting food of the present invention comprises the herbal extract in an amount of 0.01 to 95% by weight, preferably 1 to 80% by weight of the total food.

In addition, the weight ratio and manufacturing method of the herbal extracts contained in the health promoting food of the present invention is as described above.

The health promoting food of the present invention may be in the form of powder, granules, tablets, capsules or beverages, and may further include other natural or synthetic substances for health promotion and additives for commercialization.

Examples of the health promoting food of the present invention include various foods, beverages, gums, vitamin complexes, and health supplements.

The present invention also provides a hangover drink containing the herbal extract as an active ingredient.

The beverage of the present invention comprises the herbal extract in an amount of 0.01 to 95% by weight, preferably 1 to 80% by weight of the total composition.

In addition, the weight ratio and manufacturing method of the herbal medicines included in the beverage of the present invention are as described above.

Health drink of the present invention may contain the herbal extract in the ratio of 1 to 100g based on 100ml. In addition, if the extract contains the extract as an essential ingredient in the indicated ratio, there is no particular limitation on the liquid component, and may contain various flavors or natural carbohydrates, etc. as additional ingredients, as in general beverages. Examples of the above-mentioned natural carbohydrates include monosaccharides such as glucose, fructose and other disaccharides such as maltose, sucrose and the like, and conventional sugars such as polysaccharides such as dextrin, cyclodextrin, And sugar alcohols such as xylitol, sorbitol, and erythritol. As flavoring agents other than those mentioned above, natural flavoring agents (tauumatin, stevia extract (for example, rebaudioside A, glycyrrhizin, etc.) and synthetic flavoring agents (saccharin, aspartame, etc.) can be used.

In addition to the above, the health-enhancing food for hangover of the present invention includes various nutrients, vitamins, minerals (electrolytes), synthetic flavors such as synthetic flavors and natural flavors, coloring and neutralizing agents (such as cheese, chocolate), pectic acid and salts thereof. , Alginic acid and salts thereof, organic acids, protective colloidal thickeners, pH adjusters, stabilizers, preservatives, glycerin, alcohols, carbonation agents used in carbonated drinks and the like. In addition, the health promoting food of the present invention in the form of a beverage may contain pulp for the production of natural fruit juice and fruit juice beverage and vegetable beverage. These components can be used independently or in combination. The proportion of such additives is generally selected from the range of 1 to about 90 parts by weight per 100 parts by weight of the health promoting food of the present invention.

Hereinafter, the present invention will be described in more detail with reference to the following examples and test examples. However, the following Examples and Test Examples are only for illustrating the present invention, but the scope of the present invention is not limited thereto.

Example 1 Preparation of Extract

Extracts of each of dried, soft, earthy and browned powders were obtained through hydrothermal leaching after adding water to each of them, as shown in Table 1 below. Natural raw materials were all used in a dried state.

Example 2: Preparation of Hangover Composition

After drying at a rate of 70 g / L dry (including skin), 15 g / L soft skin, and 15 g / L soft earth (including fruit), dried, hydrothermal leaching for 6 hours to obtain a mixed composition A. And, after mixing the dry, softened, earthed and browned at the rate of dry 25g / L, softened meat 15g / L, earthen (including fruit) 15g / L and browned 10g / L, and mixed for 6 hours by hydrothermal leaching Obtained.

Test Example 1: Method for Measuring ADH Activity

Functional Test Guide of Functional Foods Modified by Blandino's Method (Blandino, A. et al., Biotech. Letts., 19 (7), 65118, 1997) as the Index of NADH Production at Absorbance 340nm (Ⅰ) Based on the method described in the Korea Food and Drug Administration, March 2004, the enzyme source was changed to yeast ADH.

Test Example 2: ALDH Activity Measurement Method

Functional test guide (I) of health functional foods modified from Bostian's method (Bostian. Et al., Biochem. J. , 173, 787, 1978) as an indicator of the rate of NADH production at absorbance of 340 nm (Food and Drug Administration, The method described in March 2004) was used.

Test Example 3 ADH and ALDH Activity Promoting Effect of Dried Extract

In order to examine the effect of promoting ADH and ALDH activity of the dried extract, by measuring the increase rate of ADH and ALDH activity of the dried extract of Example 1 (external coating, 60g / L) using the method of Test Examples 1 and 2 Tables 2 and 3, respectively, are shown.

As a result, the dryness including the skin showed the effect of increasing the ADH and ALDH activity.

Experimental Example 4: ALDH Activity Promoting Effect of Lotus Root Extract

In order to determine the effect of promoting the ALDH activity of the extract of the soft lotus, the increase rate of ALDH activity of the soft extract of Example 1 (22g / L) of Example 1 using the method of Test Example 2 is shown in Table 4.

As a result, the extract of lotus root meat showed an effect of increasing ALDH activity.

Test Example 5: ADH and ALDH activity promoting effect of the extract

In order to determine the effect of promoting ADH and ALDH activity of the extract extract, after diluting the extract (34g / L) of Example 1 using the method of Test Examples 1 and 2 by measuring the rate of ADH and ALDH activity increase Tables 5 and 6 show, respectively.

As a result, the extract of the earth showed an effect of increasing the ADH and ALDH activity.

Test Example 6 Effect of Promoting ADH and ALDH Activity of Gall Powder Extract

In order to determine the effect of promoting the ADH and ALDH activity of the galvanized extract, by diluting the galvanized extract of Example 1 (50g / L) using the method of Test Examples 1 and 2, respectively, the rate of increase in ADH and ALDH activity was measured. 7 and 8 are shown.

As a result, the extract of browning showed an effect of increasing ADH and ALDH activity.

Experimental Example 7: ADH activity promoting effect of the hangover relief composition

In order to determine the effect of promoting the ADH activity of the hangover relief composition, by using the method of Test Example 1, the growth rate of ADH activity of the hangover drink and the hangover composition A and B of Example 2 were measured, respectively, to Table 9 and 10 is shown.

As a result, the composition A of Example 2 exhibited a relatively high ADH activity promoting effect, composition B showed a more than the commercially available ADH activity promoting effect.

Experimental Example 8: ALDH activity promoting effect of the hangover relief composition

In order to investigate the ALDH activity promoting effect of the hangover relief composition, by using the method of Test Example 2 to measure the growth rate of the ALDH activity of the hangover beverage commercial products (condition) and the hangover composition A and B of Example 2 Tables 11 and 12, respectively, are shown.

As a result, both compositions A and B of Example 2 exhibited higher ALDH activity promoting effects than commercial products.

Test Example 9 Effect of Alcohol and Acetaldehyde Degradation Promoting Effect of Hangover Relief Composition in vivo  Experiment method

In vivo experiments were conducted to determine the effects of alcohol and acetaldehyde degradation on the hangover relief composition. 69 groups of 9 groups (Sample A, B, C, pre-alcohol-injected group, alcohol-administered sample and sample D, E, control group) 69 (Samples A, B, C, D, E (Sprague Dawley rat, male, 215-225 g each, 7 weeks old) were fasted for 18 hours before the experiment, and the samples A, B, C, D and E (Table 13) was administered before or after oral administration of 40 ml ethanol at 8.4 ml / kg. Sample dosages and dosing periods are shown in Table 14. In Table 14, pretreatment means that the sample is administered 30 minutes before alcohol administration, and post-treatment means that the sample is administered 30 minutes after alcohol administration.

Thereafter, 30 minutes, 150 minutes and 270 minutes after the administration of alcohol in the pretreatment, 30 minutes, 150 minutes and 270 minutes after the administration of the sample in the post-treatment, the blood was collected by partial blood collection. Thereafter, serum was separated by centrifugation at 3,000 rpm and 20 min, and blood alcohol concentration and acetaldehyde concentration were measured through a kit test (Ethanol & Acetaldehyde Kit ( r- Bioform, Roche)). The measurement method used the manufacturer's protocol.

Test Example 10: Promoting Alcohol Degradation of Hangover Relief Composition in vivo  effect

Table 15 to 18 shows the effect of reducing the blood alcohol concentration of each sample measured in Test Example 9.

AUC (Area Under Curve) analysis: Area under the drug concentration-time curve in blood, indicating the degree of bioabsorption of the drug and reflecting the total amount of active drug that has reached systemic circulation.

In Table 18, it was expressed as +, when there is no statistical significance compared to the control group-(same as below).

As a result of animal experiments using blood alcohol concentration, when comparing sample C and sample E, dried, soft meat, earth and browning extract showed an excellent alcohol degradation promoting effect compared to commercial products.

Experimental Example 11: Accelerating Acetaldehyde Degradation of the Hangover Relief Composition in vivo   effect

Table 19 to 22 show the effect of reducing the acetaldehyde concentration in blood of each sample measured in Test Example 9.

As a result of animal experiments using blood acetaldehyde concentration, when comparing samples C and D and E, dried, soft meat, earth and browned extracts showed better acetaldehyde degradation promoting effects than commercial products.

Hereinafter, the formulation example for the hangover composition of this invention is illustrated.

Formulation Example 1 Preparation of Concentrate

600 mg herbal composition of Example 2

Oligosaccharide 600 mg

Pear Concentrate 500 mg

Mixed Vitamin 30 mg

Strawberry flavor

Purified water was added to make a total of 30 ml. The above components were mixed according to a conventional method for preparing a liquid, and then filled into a polyethylene cloth and sterilized to prepare a concentrate.

Formulation Example 2 Preparation of Health Promoting Food

150 mg of the herbal composition of Example 2

Lactose 120 mg

Whey Calcium 30 mg

Vitamin D3 30 mg

Vitamin C 20 mg

Green Tea Extract Powder

Calcium pantothenate

L-cystine titrant

Xylitol Proper

Magnesium stearate appropriate amount

The composition ratio of the above-mentioned vitamin and mineral mixtures is a composition suitable for a relatively healthy food in a preferred embodiment, but the composition ratio may be arbitrarily modified, and the above ingredients are mixed according to a conventional health food manufacturing method. Granules were prepared, and health promoting foods were prepared according to conventional methods.

Formulation Example 3 Preparation of Beverage

0.25 g citric acid

Fructose 35 g

60 g of grape juice

0.2 g of lemon juice

1000 ml total by adding the herbal composition of Example 2

The above ingredients were mixed according to the conventional healthy beverage preparation method, filtered and obtained in a 100 ml container, sealed and sterilized, and then refrigerated.

Claims (8)

Hangover-relieving composition containing the extract of dried skin, lotus root meat and earth earth as an active ingredient. Hangover relief composition containing the extract of dried, soft, earth and gallium including the outer shell as an active ingredient. The method according to claim 1 or 2, The extract is characterized in that the extract is included in an amount of 0.01 to 95% by weight of the total composition. The method of claim 1, The composition of the extract, characterized in that the weight ratio of the raw material before extraction of the dry, soft meat and the earth is 1.0-8.0: 0.75-3.0: 1. The method of claim 2, The composition of the extract, characterized in that the weight ratio of the raw material before extraction of the dry, soft meat, earth and browning is 1.3-16.0: 0.7-6.0: 0.6-4.0: 1. delete Hangover-relieving drink containing extracts of dried skin, lotus root meat and earthworms containing the outer shell as an active ingredient. Hangover-relieving drink containing extracts of dried skin, lotus root meat, earth and gallium, including the shell as an active ingredient.