KR101247927B1 - Composition for prevention or relief of alcoholic hangover - Google Patents

Abstract

PURPOSE: A composition for preventing or relieving an alcoholic hangover is provided to have excellent alcohol decomposition ability and acetaldehyde decomposition ability. CONSTITUTION: A composition for preventing or relieving an alcoholic hangover comprises a water caltrop extract, a fermented extract of Artemisia capillaris Thunb, green leaves, mulberry leaves, balsam pear, kuzu vine, and lotus root, in which the Artemisia capillaris Thunb, green leaves, mulberry leaves, balsam pear, kuzu vine, and lotus root are fermented with a mixed spawn of Streptomyces thermophilus, Lactobacillus acidophilus, Saccharomyces cerevisiae, and Bacillus subtilis in a weight ratio of 1:1:1:1; and a rice embryo complex fermented extract, at a weight ratio of 1:75-175:1-8. The water caltrop extract is extracted with water, alcohol with 1-4 carbons, ethylacetate, chloroform, hexane, dichloromethane, or their mixture. The rice embryo complex fermented extract is obtained by fermenting rice embryo, rice bran, and soybean-originated peptide by at least one microorganism selected from the group consisting of Saccharomyces microorganisms, and Issatchenkia microorganisms. The Saccharomyces microorganism is at least one selected from the group consisting of Saccharomyces cerevisiae, Saccharomyces cerevisiae, and Saccaromyces bayanus. The issatchenkia microorganism is at least one selected from the group consisting of Issatchenkia orientalis and issatchenkia occidentalis. [Reference numerals] (AA) Alcohol; (BB) Rice embryo complex fermented extract; (CC) Caltrop + plant fermentation; (DD) Caltrop + plant fermentation + rice embryo complex fermented extract; (EE) Plant fermentation + rice embryo complex fermented extract; (FF) Caltrop + rice embryo complex fermented extract; (GG) Yeo-myoung 808(existing commercial product); (HH) Caltrop 400; (II) Plant fermentation

Description

Composition for prevention or relief of alcoholic hangover}

The present invention relates to a composition for preventing or eliminating hangovers.

Alcohol is on the rise in consumption to relieve stress of modern people. However, drinking large amounts of alcohol causes symptoms such as nausea, vomiting, dizziness, thirst, lethargy, headache, and muscle aches from alcoholic hangovers, resulting in socioeconomic damages due to poor work efficiency (Swift R, Davidson D). Alcohol hangover: mechanisms and mediators.Alcohol Health Res World, 22 (1): 54-60, 1998).

Alcohol is metabolized through three pathways when absorbed into the body.At low alcohol levels, alcohol is present in the endoplasmic reticulum by the action of ADH (alcohol dehydrogenase) and ALDH (acetaldehyde dehydrogenase) in the gastrointestinal tract or liver. It is metabolized to acetaldehyde and acetic acid by the microsomal ethanol oxidizing system (MEOS), and then finally decomposed into carbon dioxide and water through the action of catalase present in the peroxisome (Korea) Korean Patent Publication No. 10-2012-0016688, February 27, 2012, paragraph [0005].

When the proper amount of alcohol is introduced, the metabolic system works smoothly and does not cause any symptoms caused by alcohol, but when the large amount of alcohol is introduced, the balance of the metabolic system is destroyed and thus the homeostasis cannot be maintained. Acetaldehyde, a primary metabolite made by oxidizing alcohol, is a major factor in hangover, and the effects of hangover by acetaldehyde are greater than alcohol itself through alcohol toxicity. Acetaldehyde produced in the body is transmitted to the brain and converted into many harmful compounds, which can cause symptoms such as increased pulse, sweating, flushing, nausea, and vomiting (Korea Patent Publication No. 10-2012-0016688, Feb. 27, 2012, Paragraph [0006].

Therefore, in order to effectively solve the alcoholic hangover in a short time, it is required to develop a composition having excellent alcohol decomposing ability and acetaldehyde decomposing ability which can effectively decrease the concentration of acetaldehyde with the decrease of the alcohol concentration in the body. This is a composition that has good alcohol decomposing ability but not acetaldehyde decomposing ability, and may be ineffective in relieving hangover due to accumulation of acetaldehyde in the body, and in the case of a composition which does not have good alcohol decomposing ability but excellent acetaldehyde decomposing ability. Because it takes a long time to decompose all the alcohol in the body, even low levels of acetaldehyde in the body may be exposed for a long time may not be effective.

Korea Patent Publication No. 10-2012-0016688, February 27, 2012, [0005]-[0006] paragraph

Swift R, Davidson D, Alcohol hangover: mechanisms and mediators. Alcohol Health Res World, 22 (1): 54-60, 1998

Accordingly, the present inventors have tried to find a composition that is effective in preventing or eliminating hangovers because of excellent alcohol decomposing ability and acetaldehyde decomposing ability, and thus (a) dried extract, (b) plant fermentation extracts of jinjin wormwood, green leaf, mulberry leaf, bitter gourd, 칡 and lotus root. And (c) found that the composition containing the embryo embryo complex fermentation extract has excellent alcohol decomposing ability and acetaldehyde decomposing ability and completed the present invention.

Accordingly, an object of the present invention is to provide a composition effective in preventing or eliminating hangover due to excellent alcohol decomposing ability and acetaldehyde decomposing ability.

In order to achieve the above object, the present invention (a) dried extract, (b) jinjin mugwort, green leaf, mulberry leaf, Yeoju, 칡 and lotus root plant fermentation extract and (c) embryo embryo complex fermentation extract preventing or eliminating It provides a composition for.

Dermis is Trapa , belonging to the Hydrocaryaceae family japonica Flerov or other cognate plant. Marm is an annual herb that grows in water. About 15% of the walnut fruit, which is a rhombus, is composed of starch. In addition, it contains glucose, protein, vitamin C, and vitamin B, and has a slightly sweet taste.

In the present invention, dried is also included in the scope of the present invention processed natural dry, such as semi-dry, drying. Also, in the present invention, the whole or part of the dried leaves may be used, for example, leaves, stems, roots, fruits, seeds, etc. of the dried leaves, and preferably dried leaves may be used.

In the present invention (a) the dry extract may be prepared by extracting the extract itself with a conventional extraction method, for example, hot water extraction or an organic solvent, as it is or in the form of finely divided or powdered. In addition, the dried extract of the present invention may be a dry powder obtained by drying the extract or commercially available (a) dried extract powder. In the preparation of the extract, dried, undried, or a mixture of them can be used. (a) The dried extract is, for example, after cutting the dried herbal medicine, about 3 hours to 1000 times the weight of water, organic solvent or a mixed solvent thereof at room temperature to 60 ~ 130 ℃ extraction temperature for about 1 hour After extracting repeatedly from 1 to 5 times by an extraction method such as hot water extraction, cold sediment extraction, ultra-high pressure extraction or reflux cooling extraction for from 10 days, the extract may be prepared by filtration, concentrated under reduced pressure or lyophilized. Examples of the organic solvent include alcohols having 1 to 4 carbon atoms, ethyl acetate, chloroform, hexane, dichloromethane, and the like.

In the present invention (a) the dry extract may be extracted with water, alcohol having 1 to 4 carbon atoms, ethyl acetate, chloroform, hexane, dichloromethane or a mixed solvent thereof.

In the present invention (b) the plant fermentation extracts of jinjin mugwort, green leaves, mulberry leaves, bitter gourd, 칡 and lotus root (hereinafter referred to as plant fermentation extract) is Streptomyc thermophilus ( Streptomycesthermophilu s) , Lactobacillus Ecija FIG filler's (Lactobacillusacidophilus), saccharose in my process three Levy jiae (Saccharomycescerevisiae), B. subtilis (Bacillussubtilis) a 1: 1: 1: 1 into effect obtained by fermentation by a mixture of microorganisms mixed in a weight ratio of Extract.

Inemisia Artemisia belongs to the Asteraceae capillaris Or other homologous plants. It is also called wormwood or heat keeper, and the herbal name is Injin Ho. Injin mugwort is also called Sajeolbong, Chosun Injinho, Sineho, Injin, Whiteho, Gainjin, Seokjinjin, Injingo, and Danggangmugwort. Injin mugwort was discovered in ancient China by the name of Hua Ta, and used as a herbal medicine in oriental medicine. It is a perennial herb, 30-100 cm long, completely divided into idols, and yellow flowers bloom between August and September and mature around October. Young sprouts and leaves have been used as mugwort rice cakes and mugwort teas since ancient times.

Injin mugwort in the present invention is a natural state, semi-dried, dried and processed injin mugwort is also included in the scope of the present invention. For example, in the present invention, the jinjin mugwort may use a lyophilized powder form. In the present invention, the jinjin mugwort may be used in whole or in part of the jinjin mugwort, preferably jinjin mugwort.

In the present invention is nokyeop Thea belonging to theaceae (Theaceae) sinensis L. or other homogenous root plant. Green leaves are also commonly called green tea leaves. Green leaf is a food material widely used at home and abroad and has been ingested mainly in the form of tea.

Water or alcohol extract of green tea is currently used as a raw material of food, it is known that the use of antioxidants, toxin removal effect, vascular disease prevention effect, cancer prevention effect and the like.

In the present invention, the green leaf is processed in the natural state, semi-dried, dried green leaves also fall within the scope of the present invention. For example, the green leaves in the present invention may use a lyophilized powder form.

Mulberry is Morus belonging to Moraceae alba Linne or other related plant leaves.

In the present invention, the mulberry leaves in the natural state, semi-dried, dried and processed mulberry leaves are also included in the scope of the present invention. For example, in the present invention, the mulberry leaf may use a lyophilized powder form.

The hostess is Momordica belonging to the family Cucurbitaceae . charantia Linn or other cognate plant. Yeoju is native to tropical Asia and is a vine annual herb. It is commonly called bitter melon or balsam pear. Stems are thin, grow to 3-7m long, and may grow larger. The leaves are alternate, the petioles are long, the fruits are long oval, the both ends are narrow, hump-like projections, and when the green or ripening ripens to yellow-green, light green, yellow-red, the seeds are irregularly divided and wrapped in a crimson flesh. Yeoju grows or grows naturally throughout Korea.

In the present invention, the processed bitter gourd, such as natural state, semi-dry and dried, is also included in the scope of the present invention. For example, the litchi in the present invention may use a lyophilized powder form. In addition, in the present invention, all or part of the bitter gourd, such as leaves, stems, roots, fruits, seeds, etc. of the bitter gourd may be used, and preferably the fruit of the bitter gourd may be used.

Pueraria belonging to the family Legume ( Fabaceae / Leguminosae ) lobata Ohwi or other cognate plant. 칡 is a deciduous vine that grows in the foothills of Korea. The leaf is composed of 3 leaflets of double leaf, the small leaves are broad ovate, flat or shallow at 2-3 edges, the flowers are genus inflorescences, attached to the axillas and fuchsia, and the fruits are nectar, linear, and brown. Bristles are dense, 4-9 cm long. Blooming season is August and fruiting season is September-October.

In the present invention, 칡 is processed in the natural state, semi-drying, drying and the like is also included in the scope of the present invention. For example, in the present invention, thin lyophilized powder may be used. In addition, in the present invention, 칡 may be used in whole or in part, such as leaves, stems, roots, fruits, seeds, etc. of the 칡, preferably the root of 칡 may be used.

Lotus root is Nelumbo belonging to the family Nelumboaceae nucifera Root stem of Gaertn or other cohort of related plants.

In the present invention, the lotus root is processed in the natural state, semi-dried, dried, etc. also fall within the scope of the present invention. For example, in the present invention, lotus root may be used as a lyophilized powder.

In the present invention (b) plant fermentation extract is a mixture of Streptomyces thermophilus, Lactobacillus ecidophilus, Saccharomyces cerevisiae, Bacillus subtilis in a weight ratio of 1: 1: 1: 1 Spawn preparation step for preparing spawn; 1 to 1 part by weight of the mixed seedlings and 50 to 200 parts by weight of phosphorus mugwort based on 1 part by weight of the mixed seedlings, 50 to 250 parts by weight of green leaves are mixed and put into a container, and the container is supplied with oxygen using a bubbler for 2-4 months. A primary culture step of preparing a primary culture by culturing at room temperature; Prepare 50 to 200 parts by weight of phosphorus mugwort, 50 to 200 parts by weight of green leaves, 50 to 200 parts by weight of mulberry leaves, 50 to 250 parts by weight of bitter gourd, 50 to 100 parts by weight, and 25 to 75 parts by weight of lotus root based on 1 part by weight of the mixed seed. Secondary culture step of preparing a secondary culture solution by mixing in the primary culture solution and incubated at room temperature for 1 to 2 months in the same environment; And it can be prepared by a process comprising a aging step of aging the secondary culture at room temperature for 1 to 2 months.

In the present invention, room temperature means 15 to 25 ℃.

The process may further comprise a filtration and concentration step of filtering and concentrating the secondary culture.

The filtration is preferably filtered by adding water and carbohydrate degrading enzyme to the secondary culture in order to enhance the filtration efficiency.

The carbohydrate degrading enzymes include Promozyme, Cellluclast, Maltogenase, Maltogenase, Biscozyme, Teramyl, Dextrozyme, Ultraflo. And one or more selected from the group consisting of AMG may be used, preferably Teramyl and Biscozyme are mixed in the same weight ratio, wherein the carbohydrate decomposition for a total of 100 parts by weight of the fermentation culture It is preferable to include the enzyme in 0.001 to 1 parts by weight.

In the present invention (c) the embryo embryo complex fermentation extract is fermented by at least one microorganism selected from the group consisting of Saccharomyces ( Saccharomyces ) and Isschenchen genus microorganisms derived from the embryos, rice bran and legumes Fermented extract obtained.

In the present invention (c) the embryo complex composite fermentation extract is 1-10% by weight of embryos, 1-10% by weight of rice bran, 0.1-5% by weight of peptides derived from legumes, 0.1-5% by weight of phytic acid, 0.5-5% by weight of sodium diphosphate %, Potassium diphosphate 0.05 to 2% by weight, glucose 0.5 to 5% by weight, antifoaming agent 0.001 to 0.1% by weight and the balance of water in the group of Saccharomyces genus and Issatchenkia genus It can be obtained by fermentation by one or more microorganisms selected.

The rice bran may be skim rice bran, the legume-derived peptide may be a soybean peptide.

Saccharide in my process in the microorganism Saccharomyces as MY process three Levy jiae (Saccharomycescerevisiae), saccharose in my process bowl radio (SaccharomycesBoulardii), saccharose in my process bar Janus (Saccharomycesbayanus) and the like, preferably a saccharide as MY process three It can be Leviathan.

Moving may be Escherichia Chen (Issatchenkia) in the microorganism Escherichia director Chen Oriental less (Issatchenkiaorientalis), director Chen Escherichia Occidental less (Issatchenkiaoccidentalis) is incorporated and the like, preferably moving Chen Escherichia Oriental less MFST1 (IssatchenkiaorientalisMFST1).

The microorganism of the genus Saccharomyces is cream tan, and the microorganism of the genus Issatchenkia is cream white. Both microorganisms are a type of diffuser yeast that breed by germination and are resistant to heat and predominate when fermentation is above a certain temperature during fermentation process. It has the advantage of receiving less and changing quality due to various germs.

The fermentation is preferably incubated for 24 to 72 hours at a temperature of 25 to 35 ℃ when using the Saccharomyces genus, 35 to 45 ℃ when using the genus Issatchenkia Incubation for 40 to 52 hours is preferred.

In the present invention, the embryo complex composite fermentation extract is 1-10% by weight of embryos, 1-10% by weight of rice bran, 0.1-5% by weight of peptides derived from legumes, 0.1-5% by weight of phytic acid, 0.5-5% by weight of sodium diphosphate, and A raw material mixing step of adding and stirring a protease to a raw material including 0.05 to 2 wt% of potassium phosphate, 0.5 to 5 wt% of glucose, 0.001 to 0.1 wt% of an antifoaming agent, and the balance of water; A fermentation step of inoculating the raw material mixture with one or more microorganisms selected from the group consisting of Saccharomyces genus and Issatchenkia genus microorganisms; And filtration and concentration steps of filtering and concentrating the fermentation culture.

The filtration is preferably filtered by adding water and carbohydrate degrading enzyme to the fermentation culture in order to enhance the filtration efficiency.

The carbohydrate degrading enzyme may be one or more selected from the group consisting of promozyme, cellulose, maltogenase, biscozyme, thermamil, dextrozzyme, ultraflo and AMG, preferably thermamil and bisco Zyme is used by mixing in the same weight ratio, and it is preferable to include carbohydrate degrading enzyme in 0.001 to 1 parts by weight based on 100 parts by weight of the fermentation culture.

The protease is preferably added in an amount of 0.001 to 1 part by weight based on 100 parts by weight of the raw material.

In the present invention, the weight ratio of (a) dry extract, (b) jinjin mugwort, green leaf, mulberry leaf, Yeoju, 칡 and lotus root of plant fermentation extract and (c) ungerminated composite fermentation extract of the hangover prevention composition in the present invention is 1:75 ~ 175: 1 to 8, for example 1: 125: 2.

In the present invention, the composition for preventing or eliminating hangover is effective in preventing or eliminating hangover in addition to (a) dried fermented extracts, (b) plant fermentation extracts of green leaves, mulberry leaves, bitter gourds, silkworms and lotus roots, and (c) complex fermented extracts of embryonic embryos. Known natural products or known extracts thereof, or active functional substances may be further included. These components can be used independently or in combination.

Known natural products or extracts thereof that are known to be effective in preventing or eliminating hangovers are turmeric, turmeric fruit, black soybeans, golden, wolfberry, yeast, wasabi, plantain, fresh vinegar, mugwort, buttercup, bean sprout, persimmon leaf, root , Bamboo, alder, citrus, astragalus, spot, prickly pear or extract thereof.

Active functional substances known to be effective in preventing or eliminating hangovers may be taurine, L-aspartic acid, glutathione, nicotinic acid amide, fumaric acid, propolis, metaonine, betaine and the like.

The ratio of the known natural products or extracts thereof and the active functional substances which are known to be effective in preventing or eliminating the hangover is not so important but is generally selected from 1 to about 90 parts by weight per 100 parts by weight of the composition of the present invention.

Hangover in the present invention means an alcoholic hangover showing symptoms such as nausea, vomiting, dizziness, thirst, lethargy, headache, myalgia due to large intake of alcohol.

Prevention in the present invention means any action that inhibits or delays hangover by administration of the composition according to the present invention.

Resolving in the present invention means any action that improves or advantageously changes the hangover by administration of the composition according to the present invention.

The present invention is also for preventing or eliminating hangovers comprising (a) dried extract, (b) jinjin mugwort, green leaf, mulberry leaf, Yeoju, 칡 and lotus root fermentation extract and (c) embryo fermentation extract according to the present invention Provide dietary supplements.

In the present invention, the health functional food is fully established in the functional and safety for the human body newly defined through the law on health functional food in 2002, the health functional food raw materials or ingredients recognized in the Korea Food and Drug Administration Notice 2004-12 It is characterized by health foods listed in the regulation list.

The health functional food for preventing or eliminating hangovers may further comprise food acceptable additives.

As foods to which (a) dried extract of the present invention, (b) plant fermentation extracts of jinjin wormwood, green leaves, mulberry leaves, bitter gourd, 칡 and lotus root, and (c) composite fermented extract of embryonic embryos, various foods, for example For example, beverages, gums, teas, vitamin complexes, dietary supplements, and the like, may be used in the form of pills, powders, granules, acupuncture tablets, capsules, or beverages.

At this time, the amount of the (a) dried extract, (b) jinjin mugwort, green leaf, mulberry leaf, bitter gourd, lotus root and lotus root fermentation extract and (c) micro-fermented fermentation extract in food or beverage is generally the health food of the present invention Food may be included in 0.01 to 95% by weight, preferably 0.1 to 80% by weight of the total food weight, in the case of a health beverage composition may be included in a ratio of 0.1 to 100g based on 100ml.

The health beverage composition of the present invention contains the plant fermentation extracts of (a) dried leaf extract, (b) jinjin mugwort, green leaf, mulberry leaf, bitter gourd, lotus root and lotus root as essential ingredients in the indicated ratio, and (c) the embryo fermented complex fermentation extract. There is no particular limitation on the liquid component except for the above, and it may contain various flavors or natural carbohydrates and the like as additional ingredients, like ordinary drinks.

Examples of the above-mentioned natural carbohydrates include monosaccharides such as glucose, fructose and other disaccharides, oligosaccharides such as maltose and sucrose polysaccharides such as dextrins and cyclodextrins. And sugar alcohols such as xylitol, sorbitol, and erythritol. As natural flavors other than those described above, natural flavors (such as tau martin, stevia extract (e.g., rebaudioside A, glycyrrhizin)) and synthetic flavors (saccharin, aspartame, etc.) have.

In addition to the above, the composition of the present invention includes various nutrients, vitamins, minerals (electrolytes), flavors such as synthetic flavors and natural flavors, coloring and neutralizing agents (such as cheese and chocolate), pectic acid and salts thereof, alginic acid and salts thereof. Organic acids such as citric acid, protective colloidal thickeners, pH adjusters, stabilizers, preservatives, glycerin, alcohols, carbonation agents used in carbonated drinks and the like. In addition, the compositions of the present invention may contain flesh, concentrates for the production of natural fruit juices and fruit juice beverages and vegetable beverages. These components can be used independently or in combination. The proportion of such additives is not so critical but is generally selected in the range of 1 to about 90 parts by weight per 100 parts by weight of the composition of the present invention.

The composition according to the present invention has excellent alcohol decomposing ability and acetaldehyde decomposing ability, and is effective in preventing or eliminating hangovers.

1 is a graph showing the result of measuring the ethanol concentration in the plasma sample according to Example 1, the x-axis represents the elapsed time (minutes) after administration of the trigger (alcohol) and the y-axis represents the ethanol concentration.
Figure 2 is a graph showing the result of measuring the acetaldehyde concentration in the plasma sample according to Example 2, the x-axis shows the elapsed time (minutes) after administration of the trigger (alcohol) and the y-axis shows the acetaldehyde concentration.

Hereinafter, the present invention will be described in more detail with reference to the following examples and experimental examples. However, the following examples are not intended to limit the scope of the present invention, which will be construed as to aid the understanding of the present invention.

Manufacturing example  1. Preparation of Dried Extract

180 g of dried dried ginseng from Gyeongdong Market was placed in 3 L of water and extracted with hot water for 6 hours at an extraction temperature of 100 ° C. The extract was filtered and concentrated, and then lyophilized to obtain a white powder.

Manufacturing example  2. Inosugi Preparation of Plant Fermentation Extracts (Plant Fermentation Extracts) of Green, Green Leaves, Mulberry Leaves, Yeoju, Fructus and Lotus Root

Injin mugwort, green leaf, mulberry leaf, Yeoju, 칡 and lotus root obtained from Gyeongdong market were washed and then drained and heat-treated in a hot air dryer at 60 ° C. for 2 hours and then lyophilized to obtain a powder form. 100 g of phosphorus mugwort in the form of lyophilized powder and 100 g of green leaves were mixed, and 8L of distilled water was added thereto, followed by stirring to mix the raw materials.

Streptomyces thermophilus (ATCC19282), Lactobacillus essidophilus (KCTC3142), Saccharomyces cerevisiae (KCTC7904) and Bacillus subtilis (KCTC1022) were added to the raw material mixture in a 1: 1: 1: 1 ratio. 1 g of the mixed seed mixed in the weight ratio of and then incubated for 3 months at room temperature with agitation (120rpm) to prepare a primary culture.

50g of the lyophilized powder, green leaf 50g, mulberry leaf 100g, Yeoju 100g, 및 100g and lotus root 50g into the primary culture solution and 4g of distilled water was added, followed by incubation at room temperature for 2 months (120rpm) 2 Primary culture was prepared.

The culture was compressed to treat activated carbon and filtered with diatomaceous earth. In order to facilitate filtration, the cultures were hydrolyzed in 5-fold portions, and a mixture of termamyl and biscozyme (Novozymes, Denmark) in a 1: 1 ratio was added at 0.001% by weight. The filtered culture solution was concentrated to a solid content of more than 30% in a water bath at 45 ℃ to obtain a plant fermentation extract of dark brown liquid phosphorus mugwort, green leaves, mulberry leaves, Yeoju, 칡 and lotus root.

Manufacturing example  3. Embryonic embryo  Preparation of Complex Fermented Extract

According to Example 1 of Korean Patent No. 10-1118683, a microembryonic complex fermentation extract was prepared. Specifically, embryos 40 g, skim rice bran 30 g, soybean peptide 4 g, phytic acid 5 g, disodium hydrogen phosphate 10 g, dipotassium hydrogen phosphate 2.5 g, glucose 15 g, antifoaming agent (GS850, Kumkang Silicon Tech Co., Ltd.) 0.25 g And 0.05% (w / w) of protease (Protamex, Novozymes, Denmark) was added to the medium containing 500 g of water, and the raw materials were mixed by stirring. At this time, the soybean peptide was used by grinding with a grinder (HM-350, Hyun Dae Household Appliances Co., Korea).

1% (w / w) of Saccharomyces cerevisiae or Isachenchia orientalis MFST1 (absorbance of 2.5 or more at 660 nm) was inoculated into the medium having the medium composition, followed by aeration and agitation at 30 ° C. or 40 ° C. for 48 hours ( 120 rpm).

The culture was compressed to treat activated carbon and filtered with diatomaceous earth. In order to facilitate filtration, the cultures were hydrolyzed in 5 times the amount, and mixed with Termamyl and Biscozyme (Novozymes, Denmark) in a 1: 1 ratio and added 0.001% by weight. The filtered culture was concentrated to a solid content of more than 30% in a 45 ℃ water bath to obtain a brown liquid embryo fermentation extract.

Manufacturing example  4. Dried Extract, Fermented Plant Extract and Embryonic embryo  Composition containing a complex fermentation extract

The composition was prepared by mixing 400 mg of dried extract prepared in Preparation Example 1, 50 g of a dark brown liquid plant fermentation extract prepared in Preparation Example 2, and 0.8 g of the embryo embryo complex fermentation extract prepared in Preparation Example 3. .

Example  1. Confirmation of average blood alcohol concentration

1. Test substance, positive control substance, negative control substance and causing substance

(1) Dried Extract + Plant Fermentation Extract + Embryonic Complex Fermentation Extract

The composition comprising the dry extract, plant fermentation extract and the embryo embryo complex fermentation extract prepared in Preparation Example 4 was mixed to 50ml of distilled water to prepare a total of 100ml.

(2) dry extract

400 mg of the white powdered powder dry powder prepared in Preparation Example 1 was measured and dissolved in 100 ml of distilled water.

(3) plant fermented extract

50 g of the plant fermentation extract prepared in Preparation Example 2 was measured and mixed with 50 ml of distilled water to prepare a total of 100 ml.

(4) Microembryonic Fermentation Extract

1.6 g of the embryo fermented extract prepared in Preparation Example 3 was measured and dissolved in 100 ml of distilled water.

(5) Dried Extract + Plant Fermentation Extract

White powder powder dry powder 400mg prepared by Preparation Example 1, 50g of the plant fermentation extract prepared by Preparation Example 2 was measured and mixed in 50ml of distilled water to prepare a total of 100ml.

(6) Dried Extract + Embryonic Complex Fermentation Extract

400 mg of the white powdered powder dried powder prepared in Preparation Example 1, 0.8 g of the microembryonic complex fermentation extract prepared in Preparation Example 3 were measured, and dissolved in 100 ml of distilled water.

(7) Plant Fermented Extract + Embryonic Complex Fermented Extract

50 g of the plant fermentation extract prepared in Preparation Example 2, 0.8 g of the embryo embryo complex fermentation extract prepared in Preparation Example 3 were measured and mixed with 50 ml of distilled water to prepare a total of 100 ml.

(8) positive control and negative control

As a positive control, commercially available Dawn 808 (Grammy, Korea) was used (positive control) and water was used as a negative control (negative control).

(9) causing substances

40% alcohol (J.T. Baker, USA) was used.

2. Test equipment

Microplate Reader (Spectra Max M2, Molecular Device, Sunnyvale, CA, USA), 10, 100, 200 and 1,000 μl pipettes (Eppendorf, Germany), Centrifuge (Union 5KR, Hanil, Korea), Vortex Mixer ( VM-3000, TALBOYS, USA).

3. Test animal Nonclinical Test system )

(1) Experimental animals

Sprague Dawley Rat obtained from Hallim experimental animals was used. Age and body weight range from 7 weeks of age and 200-220 g of male.

(2) Cycle period

The experimental animals were donated to the test after purification for 7 days after acquisition.

(3) Breeding conditions

Breeding conditions were raised in the rat animal room under the conditions of temperature 23 ± 3 ℃, relative humidity 55 ± 15%, ventilation frequency 10-20 times / hour, lighting time 12 hours (lighting at 8 am to 8 pm). The polycarbonate cages (W 235 x L 380 x H 175 mm) containing silver rugs were raised to less than 3 animals / cage during quarantine and purifying periods. Feed fed to the experiment was to receive a solid feed for experimental animals sterilized by irradiation irradiated from Hallim experimental animals freely ingested.

4. Administration of Test Substance

Test substance, positive control substance and negative control substance were performed by pretreatment before administration of the trigger (alcohol). Test substance, positive control and negative control were administered 30 minutes after the administration of the trigger.

The administration route of test substance, positive control substance, negative control substance and inducing substance (alcohol) was proceeded orally. Oral administration was performed by grasping and fixing the cervical part of the test animal with one hand and directly using the oral stainless sonde with the other hand in the stomach. The dose was 1.7 mL / kg for test substance and negative control, 2.5 mL / kg for positive control and 8.4 mL / kg for 40% alcohol. In the fasting test group, the frequency of fasting was fasted at least 8 hours before the administration.

Test substance and control substance administration were as shown in Table 1 below.

[Table 1]

5. Biological Sample Analysis

(1) Collection of biological samples (plasma samples)

Blood collection was performed at the start of administration of the test substance. The blood collection time was 30% after administration of the test substance, positive control and negative control, 40% alcohol, 30, 150, 270, 390 minutes after 40% alcohol administration. About 800 μl of blood was collected from the jugular vein, centrifuged at 4,000 rpm for about 10 minutes, and the upper layer (plasma) was separated. The separated plasma was stored in a deep freezer set at −70 ° C. until analysis.

(2) Measurement and result of alcohol concentration

Plasma sample concentration analysis for alcohol was performed using a kit (r-biopharm, Roche), and the microplate reader (Spectra Max M2, Molecular Device, Sunnyvale, CA,) after treatment according to the pretreatment method according to the manufacturer's instructions. USA).

100 μl of the plasma sample was mixed into 3 ml of the reaction mixture (potassium phosphate buffer pH 9 + NAD tablet). After incubation at 20 ° C. for 3 minutes, the absorbance at 340 nm was measured (A1). 50 μl of ADH (alcohol dehydrogenase) was added to the mixed solution, incubated at 20 ° C. for 5 minutes, and the absorbance was measured at 340 nm (A2).

Alcohol Concentration = 0.7256 / 6.3 * A

A (measured change in blood) = sample (A2-A1)-blank (A2-A1)

The average value of the result of measuring the ethanol concentration in the plasma sample is shown in Table 2 and FIG.

At all blood collection periods, the dry extract + plant fermentation extract + embryo germ extract fermentation group were the extracts of the dry extract group, the plant fermentation extract group, the embryo embryo extract fermentation extract group, the dry extract + plant fermentation extract group, the dry extract + embryo fermentation complex fermentation extract It was confirmed that the concentration of ethanol in the blood was lower than that of the group, plant fermentation extract + embryo fermentation complex fermentation extract group, as well as the positive control soldier Dawn 808. In particular, as shown in FIG. 1, the dry extract + plant fermentation extract + embryo embryo fermentation extract group showed a significant decrease in ethanol concentration after 150 minutes. In addition, it was confirmed that the concentration of alcohol in the blood was significantly reduced in the dry extract + plant fermentation extract + embryo embryo fermentation extract group at 390 minutes, the final blood collection time. Therefore, the composition of the dried extract + plant fermentation extract + embryo embryo complex fermentation extract group was confirmed that the alcohol decomposition ability is significantly superior to the composition of all other groups.

[Table 2]

Example  2. Identification of Acetaldehyde Average Concentration in Residual Blood

Example 1 In Confirmation of Average Residual Blood Alcohol Concentration 5. Means of Acetaldehyde in Residual Blood were measured by the same treatment except for the measurement and results of (2) Alcohol concentration in the biological sample analysis.

Analysis of the concentration of plasma samples for acetaldehyde using a kit (Megazyme, MALTECH), after the treatment according to the pretreatment method according to the manufacturer's instructions of the kit microplate reader (Spectra Max M2, Molecular Device, Sunnyvale, CA, USA).

2 ml of distilled water was mixed into 200 µl of the plasma sample. To this was added a reaction mixture (potassium phosphate buffer pH 9). 200 µl of the NAD was added thereto and incubated at 20 ° C. for 3 minutes, and then absorbance was measured at 340 nm (A1). 50 μl of ALDH (acetaldehyde dehydrogenase) was added to the mixed solution, and incubated at 20 ° C. for 5 minutes, and the absorbance was measured at 340 nm (A2).

Acetaldehyde concentration = 116.7325 / 630 * A

A (measured change in blood) = sample (A2-A1)-blank (A2-A1)

The average value of the result of measuring the acetaldehyde concentration in the plasma sample is shown in Table 3 and FIG.

At the time of blood collection, 150 minutes, 270 components, 390 components, the dry extract + plant fermentation extract + embryo embryo fermentation extract group was the dry extract group, plant fermentation extract group, embryo embryo fermentation extract group, dried extract + plant fermentation extract group, It was confirmed that the concentration of acetaldehyde in the blood was lower than the dry extract + embryo embryo fermentation extract group, the plant ferment extract extract + embryo embryo complex fermentation extract group, as well as the positive control soldier Dawn 808. In addition, as shown in FIG. 2, the dry extract + plant fermentation extract + embryo embryo fermentation extract group was confirmed to remain low while the fluctuation range of acetaldehyde concentration after 150 minutes.

As shown in FIG. 1, the dry extract + plant fermentation extract + embryo embryo fermentation extract group significantly reduced alcohol concentration after 150 minutes, despite the fact that acetaldehyde production due to alcohol metabolism was significantly higher than in all other groups. And lower acetaldehyde concentration than all other groups means that the composition of the dry extract + plant fermentation extract + embryo embryo fermentation extract group is significantly superior to the composition of acetaldehyde than all the other compositions.

[Table 3]

The results of Examples 1 and 2 is that the composition of the dry extract + plant fermentation extract + embryo embryo complex fermentation extract group has better alcohol decomposing ability and acetaldehyde decomposing ability than the other group composition to resolve the alcoholic hangover in a short time Shows that there is.

Claims (9)

(a) water chestnut extract, (b) injinssuk, nokyeop, mulberry leaves, bitter gourd, the arrowroot and lotus root Streptomyces Thermo filler's (Streptomycesthermophilus), Lactobacillus Ecija FIG filler's (Lactobacillusacidophilus), saccharose in my process three Levy jiae (Saccharomycescerevisiae ), Plant fermentation extracts of Injin mugwort, green leaf, mulberry leaf, Yeoju, 칡 and lotus root fermented by mixed spawn mixed Bacillus subtilis in a weight ratio of 1: 1: 1: 1, and (c) embryo embryo complex Hangover prevention or remedy composition comprising a fermentation extract. The method of claim 1, wherein the (a) dried extract is a composition for preventing or eliminating hangover that is extracted with water, alcohol having 1 to 4 carbon atoms, ethyl acetate, chloroform, hexane, dichloromethane or a mixed solvent thereof. delete The method of claim 1, wherein the (c) embryo embryo complex fermentation extract is one or more microorganisms selected from the group consisting of Saccharomyces ( Saccharomyces ) and Issatchenkia genus microbial, rice bran and legume-derived peptides It is fermented by the composition for preventing or eliminating hangovers. The method of claim 4, wherein the Saccharomyces genus microorganism is at least one selected from the group consisting of Saccharomyces cerevisiae, Saccharomyces bowladi and Saccharomyces bayanus for preventing or eliminating hangovers Composition. According to claim 4, Issatchenkia genus microorganism is at least one selected from the group consisting of Ischenchen orientalis and Ischenchen oxydentalis Hangover prevention or hangover composition. (a) dried leaf extract, (b) plant fermented extracts of green leaves, mulberry leaves, bitter gourd, larva and lotus root, and (c) composite fermented extract of embryonic embryo, wherein , Mulberry leaf, Yeoju, 칡 and lotus root plant fermentation extract and (c) embryo embryo complex fermentation extract weight ratio of 1:75 ~ 175: 1 ~ 8 hangover prevention or remedy composition. (a) Dried Extract, (b) Insam Worm, Green Leaves, Mulberry Leaf, Litchi, Root and Lotus Roots ), Plant fermentation extract of Injin mugwort, green leaf, mulberry leaf, Yeoju, 칡 and lotus root fermented by mixed spawn mixed Bacillus subtilis in a weight ratio of 1: 1: 1: 1, and (c) embryo embryo complex Health functional food for preventing or eliminating hangover, including fermented extract. (a) dried leaf extract, (b) plant fermented extracts of green leaves, mulberry leaves, bitter gourd, larva and lotus root, and (c) composite fermented extract of embryonic embryo, wherein Plant fermentation extracts of mulberry leaves, Yeoju, 칡 and lotus root and (c) non-embryonic complex fermentation extract weight ratio of 1:75 ~ 175: 1 ~ 8 Hangover preventive or remedy health functional food.

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