KR101033306B1 - Herbal application - Google Patents

Abstract

The present invention has an antibacterial, anti-inflammatory, and anti-oxidant function, and is effective in inflammation and purulent skin diseases, rhinitis, and the like, and relates to a new herbal distillate for external preparations for preventing skin aging.

According to the present invention, there is provided a herbal distillate for external preparations, which is obtained by distilling the hot water extracts of the herbal medicines including pine seedlings, loss, donkey, cheonggung, white paper and antique.

Songjeol, loss, herbal distillation, external preparation, antibacterial, anti-inflammatory

Description

Herbal distillate for external preparations with antibacterial, anti-inflammatory and antioxidant functions {Herbal application}

The present invention relates to a herbal distillate for external preparations, and more particularly, has an antibacterial, anti-inflammatory and antioxidant function, and thus is effective in inflammation, purulent skin disease, rhinitis, etc., in a new herbal distillate for external preparations to prevent skin aging. It is about.

Chemicals such as steroids, antihistamines and antibiotics are used to treat inflammation and purulent skin diseases. Although these drugs have excellent anti-inflammatory and antibacterial effects, they can cause serious skin side effects when used for long periods of time, as well as resistance to these drugs, resulting in increased use of drugs or the use of stronger drugs. A problem arises.

In particular, steroids have the best anti-inflammatory and antihistamine effects, but have severe side effects and are therefore limited in certain cases. Therefore, even if you want to get a quick therapeutic effect using a steroid, there is a disadvantage that certain people, such as pregnant women or children can not use the steroid.

Due to the problems of the conventional chemicals, there is a need for the development of a therapeutic agent for inflammation and skin diseases that does not develop resistance and has no side effects.

The present invention is to solve the above problems, the objective of the present invention is a herbal ingredient has no side effects, not only resistance to the drug does not occur, but also excellent inflammation treatment and antibacterial action, antioxidant skin It is to provide a new herbal distillate for external preparation with excellent prevention effect.

According to a feature of the present invention, there is provided a herbal distillate for external preparation, characterized in that obtained by distilling the hot water extract of the herbal medicine, including the songjeol, loss, Angelica, cheonggung, white paper and the original.

According to another feature of the present invention, the process of mixing the songjeol, loss, Angelica, Cheongung, white paper, and the original and water, the process of heating the mixture to make a hot water extract, and the step of distilling the hot water extract Chinese herbal distillation for external preparations is provided.

According to another feature of the present invention, the songjeol and the loss, Angelica, Cheongung, white paper, the original to 23 ~ 27: 13 ~ 17: 4 ~ 2: 4 ~ 2: 3 ~ 1: 1.5 ~ 1 used by mixing Chinese herbal distillation liquid is provided.

Herbal distillate according to the present invention is an external drug having antibacterial, anti-inflammatory, and antioxidant functions. Therefore, when applied to the site to be treated the present invention, it is effective in the treatment of purulent skin disease such as acne, atopic skin disease, rhinitis, etc. Antioxidant action prevents skin aging and improves skin moisturizing power. In particular, the herbal distillation liquid according to the present invention is a herbal ingredient, because there is no side effect and does not cause resistance, anyone, including pregnant women and children, can be used with confidence.

       In addition, Songjeol has a strong divergence function and loss is a strong convergence function, by using a combination of the opposite properties of these properties, the side effects of each of these drugs is minimized, and each medicine is used alone Unexpected new effects are expected, and the effects of each medicine are doubled. For example, by using the songjeol and loss together, it has an excellent effect in the treatment of rhinitis, which is expected and difficult in each medicine.

Hereinafter, the present invention will be described in more detail.

The present invention relates to a herbal distillate for external preparations extracted from Chinese herbal medicine mixtures, which are composed of Sengjeol and Loss, and which are added to Angelica, Cheongung, Baekji, and Koh Bon.

Songjeol (松 節, Pini Densiflorae Nodi Lignum) is a node of pine, and it is written in the botanical tree and the imperial ridge of `` Temple of the White Pleated Wind, Parrot, and Fracture, '' and the liquor is strengthened when the liquor is eaten. Songjeol contains rosin, and the turpentine contained in rosin has bactericidal and anti-inflammatory effects, so it is effective for healing inflammation. It is reported to have a bactericidal effect.

The loss of Querci Acutissimae fructus is effective in blood donation, congestion, dysmenorrhea, testicular infection, etc. . Friedelin, β-amyrenone, lupenone, etc. contained in the loss are anti-inflammatory action, β-sitosterol, stigmasterol, compesterol, etc. are anti-inflammatory, sulfate action. Angelicae Gigantis radix is a perennial herb belonging to the obstetric family. It has the effect of hematopoiesis, landscaping pain, and diarrhea. Roots of Angelica sedation, sedative, jingyeong, lowering blood pressure, etc. When used in arthritis solution of root essential oil is said to have analgesic and anti-inflammatory effect, in oriental medicine is mainly used for recovery of postpartum anemia, gynecology, and pregnant women. Used for coughing and swelling

In addition, cheonggung (川芎, Cnidii, Rhizoma) is a perennial herb of the apiaceae family, cold elimination, hematopoietic calming, analgesic Cheongung has the effects on various symptoms such as sedation, jingyeong, anemia, and blood donation. White paper (白芷, Angelicae Dahuricae Radix) is a herbaceous herb that belongs to the obstetric family. Its effectiveness has been known for many years. Especially, mountain breeze dehumidification, vaccination, and dysmenorrhea (生肌 止 通), effective in pain and ache (牙痛), antibacterial and pain relief and promote the blood circulation of the gum is also effective.

Angelica Tenuissimae Radix is a perennial herb belonging to the ovary family and contains essential oils consisting mainly of 3-butyl phthalide and cnidilide. Many of these ancient texts have been known since ancient times, especially headache, phenomena, chills, fever, jijeoldong, analgesic, anti-influenza virus action, swelling pain, skin disease It is effective for treatment.

In particular, the present invention using the medicinal herbs, by using the songjeol and loss as the main component, the effect of the songjeol and loss is increased and the side effects caused by these medicines are reduced. Specifically, Songjeol contains a lot of essential oils such as turpentine oil, which dissipates heat, penetrates the clogging, and releases sweat. It is effective in preventing the discharge of stool, preventing diarrhea in feces, and taking sexual disorders such as oil wells and dreams. Therefore, side effects due to the divergence effect of the songjeol and the side effects of the convergence action of the loss may occur, the present invention minimizes the side effects caused by these herbs by using the songjeol and loss together. In addition, by using these medicines together can have a new effect that is difficult to expect when using each medicine alone, such as having a therapeutic effect of rhinitis that was difficult to expect in each medicine.

The present invention is produced by the following method. First, mix the songjeol, loss, donkey, cheonggung, white paper, and bone and pour water on it. Preferably, songjeol, loss donkey, cheongunggi, blank paper is mixed 23 to 27:13 to 17: 4 to 2: 4 to 2: 3 to 1: 1.5 to 1 weight ratio. When the medicinal herbs are mixed in such a ratio, side effects such as headache are minimized, skin irritation is also minimized, and the effects of the medicinal herbs are effectively reduced without being reduced.

Water is poured into a heating container and poured with boiled herbs to obtain hot water exports. This hot water export product is distilled off and the distillate is collected. In addition to water, the distillate contains volatile components such as essential oils of herbal medicines.

Example 1 Sample Preparation

20g of water is added to 150g of Songjeol, 900g of loss, 180g of Angelica, 180g of Cheongung, 120g of white paper, and 60g of old paper. 12L of Chinese herbal distillate is prepared by cooling the steam generated in the hot water container for 20 hours after the start of the hot water pouring.

The herbal distillate thus prepared is diluted or concentrated with sterile distilled water to prepare samples of various concentrations. Hereinafter, the herbal distillate obtained by cooling the steam generated in the hot water container is expressed as 100% herbal distillate, and each distillate according to the degree of dilution or concentration is relative% concentration based on the 100% herbal distillate. Indicated as. That is, 50% herbal distillate dilutes the 100% herbal distillate twice, and 200% herbal distillate dilutes the 100% herbal distillate twice.

Example 2 Antibacterial Activity

After smearing about 1 × 10 ⁶ anaerobic bacteria P. acnes onto RCM plate medium, a plurality of 10 mm diameter filter paper discs were soaked in various concentrations of herbal distillate, and the wet filter paper discs were smeared. It adhered to the plate medium. And the disc was incubated for 24 hours in a 37 ℃ incubation container in anaerobic conditions. In this way, the diameter of the clear zone in which the bacteria were not cultured was measured, and the results are shown in Table 1.

TABLE 1

Item

Diameter of Clear Zone (mm)
50% herbal distillate

20.50 ± 4.20
100% herbal distillate

30.00 ± 4.08
150% herbal distillate

31.25 ± 2.50
200% herbal distillate

38.75 ± 2.17
300% herbal distillate

51.25 ± 6.29
esthromycin

66.25 ± 20.97

As a result, the diameter of the clear zone increased as the concentration of the distillate of herbal medicine increased. The large diameter of the clear zone means that the antibacterial activity of the distillate is excellent and the growth of bacteria is suppressed. As the concentration of the herbal distillate was increased, the diameter of the clear zone was increased. Therefore, the higher the concentration of the herbal distillate was, the better the antibacterial activity was.

P. acnes is one of the major microorganisms that causes inflammation of the pores and sebaceous glands on the skin surface. By testing the antimicrobial activity of the herbal distillation solution according to the present invention to the P. acnes, it can be seen that the herbal distillation solution according to the present invention has a therapeutic effect against such skin inflammation. On the other hand, esthromycin used as a control group has a clear zone diameter of 66.25 ± 20.97, and 300% Chinese herbal distillate is 51.25 ± 6.29. .

Example 3 Anti-Integrity Experiment

THP-1, a human normal monocyte, was dispensed in 96 well plates at 1 × 10 ⁶ cells / well and heat treated at 95 ° C. for 5 minutes. Herbal distillate of 150% and 150%, respectively, were treated. After culturing the cells for 48 hours in this way, the contents of the inflammatory cytokine TNF-α and IL-8 were cultured in the culture medium using a mouse anti-TNF-α kit and a mouse anti-IL-8 ELISA kit. It measured, and the result is shown in Table 2.

TABLE 2

Item

TNF-α
IL-8
25% herbal distillate

0.238 ± 0.044
879.179 ± 420366
50% herbal distillate

0.162 ± 0.006
612.513 ± 4.441
100% Chinese Medicine Distillate

0.146 ± 0.019
502.256 ± 49.455
150% Chinese Medicine Distillate

0.143 ± 0.019
202.256 ± 71.474

       As a result, the higher the concentration of Chinese herbal distillate, the lower the concentration of TNF-α and IL-8.

The low concentrations of TNF-α and IL-8, which are markers indicating inflammation, mean that the anti-inflammatory properties are excellent, and as a result, the higher the concentration of the herbal distillate, the better the anti-inflammatory properties. P. acnes induces inflammation of acne by causing monocytes to secrete the inflammatory cytokines TNF-α and IL-8. According to the above experimental results, the herbal distillation according to the present invention inhibits the growth of P. acnes. By sterilization, it inhibits the secretion of TNF-α and IL-8, it can be seen that there is an effect to prevent inflammation.

Example 4 Antioxidant Experiment

50 ml of ethanol was added to 50 ml of sterile distilled water to dissolve 80 mg of 1,1-diphenyl-2-picrylhydrazyl (DPPH, Sigma, USA), and then divided into 180 wells in 96 wells, 25%, 50%, 100%, 150%, 200% and 300% herbal distillates were added and left at room temperature for 30 minutes. After that, the optical density was measured at 517 nm, and converted into scavenging activity. The results are shown in Table 3.

       TABLE 3

Item

DPPH quenching power (%)
25% distillate

16.98 ± 1.95
50% distillate

18.87 ± 0.95
100% distillate

31.75 ± 9.88
150% distillate

41.65 ± 2.01
200% distillate

37.14 ± 1.69
300% distillate

41.06 ± 0.62

The experimental results showed that the higher DPPH scavenging ability was observed. High DPPH scavenging ability means that the antioxidant function is excellent, it can be seen that the higher the concentration of herbal distillate, the better the antioxidant function.

Example 5 Lipid Peroxide Inhibitory Activity

0.05 ml of linoleic acid was mixed with 4 ml of 99% ethanol and 4 ml of 50 mM phosphate buffer (pH 7.0), and then 1 ml of 1%, 10%, 100%, 500%, and 1000% Chinese herbal distillate were added. It was. 0.948 ml of distilled water was added thereto to make the final volume 10 ml, and an automatic oxidation reaction was induced at 40 ° C. for 7 days. 200 μl of the reaction solution was added to a clean test tube, and 225 μl of 8.1% sodium dodesyl sulfate (SDS) solution was added thereto, followed by mixing for 5 seconds using a vortex mixer. 1.5 ml of 20% acetic acid was added thereto, 75 μl of distilled water was added, and mixed with a vortex mixer for 5 seconds. 1 ml of 1.2% thiobarbiture acid solution was added, covered with clean dry marble, and heated in a water bath for 30 minutes. Thereafter, the mixture was left at room temperature for 30 minutes, centrifuged at 3,000 rpm for 20 minutes, the supernatant was taken, the absorbance was measured at 532 nm, and converted into lipid peroxidation inhibitory. The results are shown in Table 4.

TABLE 4

Item

Lipid peroxidation inhibitory activity (%)
1% herbal distillate

12.48 ± 1.20
10% herbal distillate

19.84 ± 2.68
100% herbal distillate

32.72 ± 0.39
500% herbal distillate

45.99 ± 12.07

The experimental results showed that the higher the concentration of distillate, the higher the lipid peroxidation inhibitory ability. Cells by biochemical reaction ^{_{O 2 -, H 2 O 2}} , when oxidized by free radical, such as -OH lipid peroxide is generated and the generation including wrinkles, pigmentation, by reducing the skin moisture to cause skin aging. The herbal distillate according to the present invention is excellent in the ability to suppress the production of peroxylipid, which causes skin aging, thus delaying skin aging and improving skin moisturizing ability.

Claims (3)

Characterized in that obtained by distilling the hot water extract of the herbal medicine containing songjeol, loss, donkey, cheonggung, white paper and antiques 23 ~ 27: 13 ~ 17: 4 ~ 2: 4 ~ 2: 3 ~ 1: 1.5 ~ 1 weight ratio An external preparation pharmaceutical composition exhibiting an antibacterial or anti-inflammatory effect. The process of mixing Songjeol, Loss, Angelica, Cheongung, Cheonggi, white paper and the original with 23 ~ 27: 13 ~ 17: 4 ~ 2: 4 ~ 2: 3 ~ 1: 1.5 ~ 1 weight ratio, and heating the mixture An external preparation pharmaceutical composition exhibiting an antimicrobial or anti-inflammatory effect, comprising: making a hot water extract, and distilling the hot water extract. delete