KR100975593B1 - Organic acid for laver farming - Google Patents

Abstract

PURPOSE: A treating agent of activation for laver is provided to effectively remove other seaweed and to enhance food stability with high efficiency. CONSTITUTION: A treat agent for activation for laver is manufactured by adding citric acid to pyroligneous liquor mixture, adding hydrochloric acid, and adjusting the inorganic concentration by 25-30%. The pyroligneous liquor mixture contains 0.1-10 wt% of chiosan oligosaccharide mixture and 0.1-10 wt% of antimicrobial extract in 89-99.8 wt% of pyroligneous liquor. The chitosan oligosaccharide mixture is prepared by dissolving chitosan in 1.5-2 % of acetic acid solution, adding 0.2-0.3% of chitosan hydrolase, performing lysis at 48°C for 42 hours, and adding 0.2-2 wt% of alginic acid. The antibiotic extract is olive, cinnamon, ginger, pine tree, spearmint, or herb extract.

Description

Active ingredient for seaweed farming {ORGANIC ACID FOR LAVER FARMING}

The present invention relates to an active treatment agent for seaweed farming, which can be expected to promote the growth and color-improving effect of seaweed by effectively treating the algae, various bacteria generated in the seaweed farm.

The aquaculture is greatly affected by the natural environment such as temperature, water temperature and rainfall. In particular, if the growth of various aquaculture facilities, changes in the fishery environment due to reclamation and reclamation, and the influx of pollutants such as factory wastewater and domestic sewage increase significantly, the crops of the aquaculture show a phenomenon of crossover every year.

Production of aquaculture laver has been deteriorated due to the aging of wheat and fisheries, causing damage to varieties and deterioration of varieties. . In addition, the quality of the product is reduced by inducing the quantitative production rather than the qualitative production due to the successful sale of seaweed laver. The quality deterioration resulting from the abuse of the use of an active treatment agent is a serious condition.

In relation to this, the laver farms are using active treatment agents to promote the growth and color of laver by removing the algae of laver and laver, and improving the color of laver. The use of hydrochloric acid as an inorganic acid is preferred to the use of organic acid as an active treatment agent by recognition of the rate and efficacy of acid treatment.

In order to solve this problem, 'Kim Aquaculture Organic Acid Compounds' of Korean Laid-Open Patent Publication No. 1998-069576 (October 26, 1998), 'Organic Acid Compounds for Kim Aquaculture' of Korean Laid-Open Patent Publication No. 1998-072363 (1998.11.05), 'Organic acid composition for control of algae attachment to laver farming net' in Korean Patent Laid-Open No. 2000-0051208 (2000.08.16), 'Oil organic acid for seaweed farming for laver farming and its preparation method in Korean Patent No. 10-0478492 (2005.03.14) ', Republic of Korea Patent No. 10-0551565 (2006.02.06) has been disclosed in the' environmentally friendly seaweed organic acid treatment agent for seaweed and its use method '.

However, the prior art has a problem that the practical use is very rarely used in the field due to the decrease in the treatment efficiency of the organic acid, which is still an active treatment agent, and the industrial applicability is very low.

Korean Patent Publication No. 1998-069576 (October 26, 1998) Korean Patent Publication No. 1998-072363 (Nov. 1998) Republic of Korea Patent Publication 2000-0051208 (2000.08.16) Republic of Korea Patent Registration 10-0478492 (2005.03.14) Republic of Korea Patent Registration 10-0551565 (2006.02.06)

In order to solve the above problems of the prior art, an object of the present invention is to remove the algae and excellent cleaning effect of the algae, substantially improving the fishery utilization of the fishery industry to increase the industrial applicability, and to provide a good environment for seaweed farming It is to provide an active treatment agent for seaweed farming that can increase productivity and increase the added value of fisherman.

In order to achieve the above object,

The present invention is to adjust the concentration of the active treatment agent to 25 to 30% by adding citric acid to the wood vinegar mixture, and for the active treatment agent for seaweed farming adjusted to the concentration of inorganic acid to 8.0 to 9.5% by adding hydrochloric acid,

The wood vinegar mixture is prepared by adding 0.1 ~ 10wt% of water-soluble chitosan, 0.1 ~ 10wt% of antimicrobial species extract to 89 ~ 99.8wt% of wood vinegar, or

Chitosan oligosaccharide mixture 0.1 ~ 10wt%, antimicrobial species extract 0.1 ~ 10wt% to wood vinegar 89 ~ 99.8wt% as the main technical configuration.

And, the wood vinegar is made of zelkova, birch, chestnut, apple, pear, cottonwood, acacia, plum, peach, bamboo, sage tree of any one or two or more trees made of charcoal, The charcoal is put into a carbonization furnace to maintain charcoal at 1,000 to 1,200 ° C. to make charcoal, and the smoke produced in this process is forcedly collected at 98 to 110 ° C. and condensed. It was prepared by selecting the negative wood vinegar and filtering with filter paper,

The antimicrobial species extract is washed with water, any one or two or more antimicrobial species selected from olive, cinnamon, ginger, pine, algae grass, spearmint, persimmon oil, tansy, sage, herbs to remove foreign substances After cutting the antimicrobial species from which the foreign substances were removed to a size of 1 to 2 mm, the ethanol aqueous solution having a concentration of 30 to 60% was added thereto, and aged for 30 to 50 days, and the antimicrobial extract of the lower layer obtained by centrifuging the matured antimicrobial extract. The extracted antimicrobial extract was separated and extracted with 1-pentanol (pH 6.5) as an eluent in liquid column chromatography, and the extracted extract was centrifuged at 2,000G to 3,000G. Antibacterial extract of the lower layer,

The water-soluble chitosan is dissolved in chitosan and then insoluble using a glass filter (glass filter) to remove the insoluble, and recrystallized by adding sodium hydroxide to wash the filtrate until the precipitated crystals are neutralized with distilled water, and then ethanol , Diethyl ether, and then dried under reduced pressure at 55 ~ 65 ℃ to prepare a deacetylated chitosan, and the chitosan thus prepared was dissolved in acetic acid solution and then frozen at -65 ~ -75 ℃ 22 ~ 25 Lyophilized for hours,

The chitosan oligosaccharide mixture was prepared by dissolving chitosan in 1.5 to 2% acetic acid solution to a final concentration of 5.5 to 6%, and then adding chitosanase to enzymatically decompose chitosan oligosaccharide 98 to 46 to 50 hours for 40 to 45 hours. It is characterized in that it was prepared by adding 0.2 ~ 2wt% alginic acid to 99.8wt%.

As described above, the active treatment agent for seaweed farming according to the present invention has a high effect on the removal of algae and microbial cleaning, which has a great effect on the growth of seaweed, by promoting the growth and color-improving effect of seaweed, fishermen at work It can be used for practical use to prevent food safety and environmental damage of fisheries by preventing illegal work using hydrochloric acid anymore.

In addition, the efficacy is superior to the existing active treatment, it is highly selective, has the advantage that can shorten the working time and inexpensive.

Detailed description of the technical configuration will be described with an embodiment.

Seaweed farming has been established in the mass production system by developing and supplying breeding farming, artificial seedling and freezing net technology. However, deterioration of the productivity and quality of seaweed due to the attachment of algae due to the aging of coastal fisheries and the neglect of gimbal management In order to remove algae and various bacteria, an active treatment agent is used. However, hydrochloric acid, an inorganic acid that has been used illegally, not only affects the safety of natural foods, but also causes problems such as environmental pollution and destruction of marine ecosystems of seaweed farms. It should be used as a seaweed activating agent to reduce pollution of the fishery environment, to control pirates, to be harmless to human body, and to decompose quickly among natural systems.

In the present invention, the concentration of the active treatment agent (organic acid) is adjusted by adding citric acid to the wood vinegar mixture, and hydrochloric acid is added thereto to adjust the concentration of the inorganic acid to 8.0 to 9.5%, but the present invention is used as a laver cultivation active agent. The characteristic is in the technical configuration of the wood vinegar mixture.

The wood vinegar mixture is as described above,

The wood vinegar mixture is prepared by adding 0.1 ~ 10wt% of water-soluble chitosan, 0.1 ~ 10wt% of antimicrobial species extract to 89 ~ 99.8wt% of wood vinegar, or

Chitosan oligosaccharide mixture 0.1 ~ 10wt%, antimicrobial species extract 0.1 ~ 10wt% to 89 ~ 99.8wt% wood vinegar is prepared.

The wood vinegar mixture is to increase the effect of removing algae and cleaning of bacteria by adding 0.1 ~ 10wt% of water-soluble chitosan or chitosan oligosaccharide mixture and 0.1 ~ 10wt% of antibacterial species extract to wood vinegar, water-soluble chitosan or chitosan oligosaccharide mixture and antibacterial species extract When the addition ratio of is less than 0.1wt%, it is difficult to expect the effect of removing algae and cleaning of bacteria, and when it exceeds 10wt%, it is uneconomical due to the increase of the manufacturing cost, so the ratio of adding to wood vinegar is 0.1 ~ 10wt% It is desirable to maintain the range. In consideration of both the removal of the algae and the effect and economical efficiency of the microorganisms, the addition ratio of the water-soluble chitosan or chitosan oligosaccharide mixture and the antimicrobial species extract is preferably 2wt%.

The wood vinegar is made of zelkova, birch, chestnut, apple, pear, cottonwood, acacia, plum, peach, bamboo, gingko tree made of one or two or more trees made of charcoal, and then Was added to the carbonization furnace to maintain at 1,000 ~ 1,200 ℃ to make charcoal, and the smoke produced in this process by forced collection at 98 ~ 110 ℃ condensed wood vinegar prepared for 2 weeks, the wood vinegar of the middle layer except the upper and lower layers It was prepared by filtering by filter paper.

If the collection temperature of the smoke generated in the process of making the charcoal wood is less than 98 ℃ can collect a large amount of wood vinegar, there is a problem that the efficiency of the wood vinegar is lowered by collecting the wood vinegar similar to water, exceeding 110 ℃ If the harmful components such as tar can be increased, the collection temperature is preferably maintained at 98 ~ 110 ℃.

In general, wood vinegar is produced by removing smoke from the process of carbonizing wood at 500-700 ℃ and liquefying it in contact with the outside air, and then aging for 6 months to remove toxic and harmful substances. The main ingredient is acetic acid and other active treatments, and it is composed of minerals such as calcium, potassium, sodium, and more than 200 organic substances such as vitamins B1 and B2.

In the preparation of the antimicrobial species, the conditions for adding the antimicrobial species are 30 to 60% of ethanol concentration, 35 to 40 ℃ aging, 30 to 50 days of aging period, the concentration of ethanol first It is limited to the range in which the active ingredient contained in the species can be separated well, and the aging temperature is taken into consideration of the temperature at which the activity of the microorganism is maximized. The separation of the active ingredient is not made well, and if it exceeds 50 days, the separation of the active ingredient is almost made, so the improvement in yield cannot be expected.

Therefore, it is preferable to separate the active ingredient contained in the antimicrobial species within the range of the concentration of ethanol, the ripening temperature, and the ripening period.

Gravity acceleration for centrifugation of the extract is maintained between 2,000G ~ 3,000G. At this time, if the gravity acceleration is less than 2,000G, centrifugation is not performed well in the extract, and if it exceeds 3,000G, no clear improvement of the separation degree is observed. Therefore, the gravity acceleration is centrifuged in the range of 2,000G to 3,000G. It is preferable to separate.

The water-soluble chitosan is dissolved in nitric acid, and then removed an insoluble matter using a filter (filter), and recrystallized by adding sodium hydroxide to wash the filtrate until the precipitated crystals are neutralized with distilled water, and then ethanol, di After washing with ethyl ether, drying under reduced pressure at 55-65 ° C. to prepare deacetylated chitosan, and dissolving the chitosan thus prepared in an acetic acid solution and freezing at −65-−75 ° C. for 22-25 hours. It is prepared by lyophilization.

The chitosan is a kind of dietary fiber having a basic structure of (1-4) -linked 2-amino-deoxy-β-D-glucan and is widely distributed in shells of shellfish such as crabs and shrimps, insect skins, mushrooms and fungi. Obtained from chitin. The free cations produced by deacetylation of the —NHCOCH ₃ group bonded to carbon 2 result in various physiological activities.

The chitosan oligosaccharide mixture was prepared by dissolving chitosan in 1.5 to 2% acetic acid solution to a final concentration of 5.5 to 6%, and then chitosan oligosaccharide which was enzymatically decomposed at 48 ° C. for 42 hours by adding 0.2 to 0.3% concentration of chitosanase. It is prepared by adding 0.2 ~ 2wt% alginic acid to 98 ~ 99.8wt%.

The chitosanase is chitinase or chitosanase.

The alginic acid is a natural alginic acid extracted from seaweed (Undaria pinnatifida) and kelp (Laminaria japonica), the extraction of the natural alginic acid is hydrothermal extraction at 90 ~ 100 ℃, the extraction temperature is a temperature range in consideration of the high yield of natural alginic acid to be.

Specific examples of the mixing ratio of the wood vinegar mixture is shown in Table 1 below.

(Unit: g) Oak Wood Vinegar Water soluble chitosan Antibacterial Species Extract Formulation Example 1 89.0 6.0 5.0 Formulation Example 2 99.8 0.1 0.1 Formulation Example 3 99.7 0.1 0.2 Formulation Example 4 89.7 10 0.3 Formulation Example 5 99.7 0.2 0.1 Formulation Example 6 89.7 0.3 10 Formulation Example 7 96.0 2.0 2.0

The oak wood vinegar of Table 1 is made of oak wood charcoal, and the charcoal is put into a carbonization furnace to maintain at 1,200 ℃ to make charcoal, and the smoke generated in this process is collected by condensation at 105 ℃ after the wood vinegar 2 After standing for a day, the wood vinegar of the middle layer except for the upper layer and the lower layer was selected and filtered with filter paper to prepare oak vinegar liquid. The reason for going through the two-week stationary process of the wood vinegar manufacturing process is to remove some light oil and tar material contained in the wood vinegar.

In the case of using the chitosan oligosaccharide mixture of the water-soluble chitosan of Table 1 to be used, the same as the amount of water-soluble chitosan used in addition to wood vinegar,

The water-soluble chitosan is dissolved in nitric acid, and then removed an insoluble matter using a filter (filter), and recrystallized by adding sodium hydroxide to wash the filtrate until the precipitated crystals are neutralized with distilled water, and then ethanol, di After washing with ethyl ether and drying under reduced pressure at 60 ° C., deacetylated chitosan was prepared. The chitosan thus prepared was dissolved in an acetic acid solution and then frozen at −70 ° C. and lyophilized for 23 hours. do.

In addition, the antimicrobial hydrolyzate extract of Table 1 was washed with 3 kg of olives under high pressure water treatment to remove foreign substances, and then the olives were cut to 2.5 cm in length and added to 42% ethanol, and aged at 35 ° C. for 40 days. The aged antimicrobial extract is centrifuged at 2,500G for 50 minutes to use the antimicrobial extract taken from the lower layer.

The active treatment agent used in the conventional seaweed farming was less effective in removing algae and cleaning of bacteria, so fish farmers were reluctant to use it. Providing an active treatment agent provides a product that can be used practically in the field of fishermen, thereby increasing food stability, thereby increasing the consumer's purchasing power, thereby increasing industrial availability.

Claims (5)

In the active treatment agent for seaweed farming, in which the concentration of inorganic acid is adjusted to 8.0 to 9.5% by adding hydrochloric acid to the active agent having a concentration adjusted to 25 to 30% by adding citric acid to the wood vinegar mixture,
The wood vinegar mixture is prepared by adding 0.1 ~ 10wt% chitosan oligosaccharide mixture, 0.1 ~ 10wt% antibacterial species extract to 89 ~ 99.8wt% wood vinegar,
The chitosan oligosaccharide mixture was prepared by dissolving chitosan in 1.5 to 2% acetic acid solution to a final concentration of 5.5 to 6%, and then chitosan oligosaccharide which was enzymatically decomposed at 48 ° C. for 42 hours by adding 0.2 to 0.3% concentration of chitosanase. It was prepared by adding 0.2-2 wt% of alginic acid to 98-99.8 wt%,
The antimicrobial species extract is washed with water, any one or two or more antimicrobial species selected from olive, cinnamon, ginger, pine, algae grass, spearmint, persimmon oil, tansy, sage, herbs to remove foreign substances After cutting the antimicrobial species from which the foreign substances were removed to a size of 1 to 2 mm, the ethanol aqueous solution having a concentration of 30 to 60% was added and aged for 30 to 50 days, and the antimicrobial extract at the bottom was centrifuged. The extracted antimicrobial extract was separated and extracted with 1-pentanol (pH 6.5) as an eluent in liquid column chromatography, and the extracted extract was centrifuged at 2,000G to 3,000G. An active treatment agent for seaweed farming, characterized in that the antibacterial extract of the lower layer. delete delete delete delete