KR100936704B1 - 효소 연료전지 - Google Patents
효소 연료전지 Download PDFInfo
- Publication number
- KR100936704B1 KR100936704B1 KR1020070115130A KR20070115130A KR100936704B1 KR 100936704 B1 KR100936704 B1 KR 100936704B1 KR 1020070115130 A KR1020070115130 A KR 1020070115130A KR 20070115130 A KR20070115130 A KR 20070115130A KR 100936704 B1 KR100936704 B1 KR 100936704B1
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- Prior art keywords
- fuel cell
- enzyme
- anode
- quinone
- immobilized
- Prior art date
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- H—ELECTRICITY
- H01—ELECTRIC ELEMENTS
- H01M—PROCESSES OR MEANS, e.g. BATTERIES, FOR THE DIRECT CONVERSION OF CHEMICAL ENERGY INTO ELECTRICAL ENERGY
- H01M8/00—Fuel cells; Manufacture thereof
- H01M8/16—Biochemical fuel cells, i.e. cells in which microorganisms function as catalysts
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E60/00—Enabling technologies; Technologies with a potential or indirect contribution to GHG emissions mitigation
- Y02E60/30—Hydrogen technology
- Y02E60/50—Fuel cells
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P70/00—Climate change mitigation technologies in the production process for final industrial or consumer products
- Y02P70/50—Manufacturing or production processes characterised by the final manufactured product
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- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Engineering & Computer Science (AREA)
- Manufacturing & Machinery (AREA)
- Sustainable Development (AREA)
- Sustainable Energy (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Electrochemistry (AREA)
- General Chemical & Material Sciences (AREA)
- Inert Electrodes (AREA)
- Fuel Cell (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
Description
Claims (9)
- 금으로 되어 있는 전극을 시스타민(cystamine)으로 처리하여 아민기(NH2)를 부착한 후, 상기 아민기와 퀴논계 전자전달 매개체를 아마이드 결합을 통해 고정화한 다음, 고정화된 퀴논계 전자전달 매개체에 연료의 산화반응효소를 고정화한 애노드(anode);백금으로 되어 있는 캐소드(cathode); 및상기 효소에 의해 산화 가능한 연료, 니코틴아마이드 아데닌 디뉴클레오티드(NAD+) 및 염화칼슘(CaCl2)을 포함하는 전해질을 포함하는 효소 연료전지.
- 삭제
- 제 1항에 있어서, 상기 애노드의 아민기와 퀴논계 전자전달 매개체의 아마이드 결합 및 퀴논계 전자전달 매개체와 산화반응효소의 고정화는 엔에틸디메틸아미노프로필 카보디아미드 하이드로클로라이드(N-ethyl-3-(3-dimethylaminopropyl) carbodimide hydrochloride, EDC), dicyclohexylcarbodiimide (DCC), diisopropylcarbodiimide (DIC) 중 선택되는 어느 하나의 커플링제(coupling agent)에 의해 유도되는 것을 특징으로 하는 효소 연료전지.
- 제 1항에 있어서, 상기 연료는 젖산(lactate)이고, 상기 산화반응용 효소는 젖산 탈수소효소(lactate dehydrogenase)이며, 상기 애노드에 고정화된 퀴논계 전 자전달 매개체는 피롤로퀴놀린 퀴논(pyrroloquinoline quinone)인 것을 특징으로 하는 효소 연료전지.
- 제 1항에 있어서, 상기 전해질의 구성성분은 10-30 mM의 젖산, 10-30 mM의 NAD+ 및 1-15 mM의 염화칼슘을 포함하는 것을 특징으로 하는 효소 연료전지.
- 제 5항에 있어서, 상기 전해질의 구성성분의 함량은 통계학적 분석방법의 하나인 반응표면 분석방법에 의해 결정하는 것을 특징으로 하는 효소 연료전지.
- 제 1항에 있어서, 온도를 유지하기 위한 워터재킷(water jacket)을 더 포함하는 것을 특징으로 하는 효소 연료전지.
- 삭제
- 하기 단계를 포함하는 효소 연료전지의 제조방법:a) 금으로 된 애노드를 왕수처리한 후, 시스타민(cystamine)으로 처리하여 아민기(NH2)를 부착하는 단계;b) 엔에틸디메틸아미노프로필 카보디아미드 하이드로클로라이드(N-ethyl-3-(3-dimethylaminopropyl) carbodimide hydrochloride)와 피콜로 퀴놀린 퀴논(pyrroloquinoline quinone)을 헤페스 버퍼(HEPES buffer) 용액에서 상기 아민기가 부착된 애노드와 반응시켜 피콜로퀴놀린 퀴논이 고정화된 애노드를 얻는 단계;c) 엔에틸디메틸아미노프로필 카보디아미드 하이드로클로라이드와 젖산 탈수소효소 용액을 인산 버퍼(phosphate buffer) 용액에서 상기 피콜로퀴놀린 퀴논이 고정화된 애노드와 반응시켜 피롤로퀴놀린 퀴논과 젖산 탈수소효소가 고정화된 애노드를 얻는 단계; 및d) 상기 피롤로퀴놀린 퀴논과 젖산 탈수소효소가 고정화된 애노드, 백금으로 된 캐소드, 그리고 젖산, 니코틴아마이드 아데닌 뉴클레오티드(NAD+) 및 염화칼슘을 포함하는 전해질을 연료전지용 용기에 장착하는 단계.
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KR1020070115130A KR100936704B1 (ko) | 2007-11-12 | 2007-11-12 | 효소 연료전지 |
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KR20090048987A KR20090048987A (ko) | 2009-05-15 |
KR100936704B1 true KR100936704B1 (ko) | 2010-01-13 |
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JP2006127957A (ja) | 2004-10-29 | 2006-05-18 | Sony Corp | 燃料電池及びその製造方法 |
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JP2006127957A (ja) | 2004-10-29 | 2006-05-18 | Sony Corp | 燃料電池及びその製造方法 |
Non-Patent Citations (3)
Title |
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논문1: CURRENT SCIENCE* |
논문2: J. Am. Chem. Soc* |
논문3: 화학공학의 이론과 응용 |
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