KR100926047B1 - Composition for Improving a Atopy Epidermal Inflammation - Google Patents

Abstract

The present invention discloses an atopic dermatitis improver composition.

Specifically, the present invention discloses an atopic dermatitis improving agent composition comprising a rice bran fermentation product obtained by fermenting rice bran powder with Aspergillus duck, Lactobacillus vulgaris and / or Streptococcus thermophilus.

Atopy, Rice Bran Ferment

Description

Composition for Improving a Atopy Epidermal Inflammation}

The present invention relates to an atopic dermatitis improving agent composition.

Atopy is derived from the Latin word "atopia," meaning "weird." Atopic dermatitis is a disease characterized by severe itching and chronic skin rashes, the most common form of infantile eczema.

The cause of atopic dermatitis is not yet clearly identified, but a person with atopic dermatitis usually has an allergic reaction to foreign objects such as house dust, mites, animal hair, food, pollen or mold. In addition, it is estimated that atopic dermatitis is caused by disturbance of immune system in that it is accompanied by allergic rhinitis, asthma, conjunctivitis, enteritis.

Atopic dermatitis usually begins with infantile eczema, commonly referred to as febrile fever, in the acute phase itching, redness, swelling, swelling like millet, small blisters, moist, scaby, and itchy skin during chronic periods. This can cause symptoms such as hardening, thickening, thinning, or rice scales such as sitting, blacking, or whitening.

Among the currently used therapeutic drugs, steroid-based drugs have excellent anti-inflammatory and immunosuppressive effects, but when used for a long time, they cause side effects such as hairs on the skin of the right area, atrophy of the skin, less skin pigment, and thinner skin.

Recently, in order to minimize side effects of existing chemicals, efforts have been made to find natural extracts effective for atopic dermatitis. For example, mixed extracts of red ginseng, baekrye, jimo and leaflet veins are used as active ingredients in treating atopic dermatitis. Korean Patent No. 0513659, Korean Patent No. 0584039, which discloses vine tea extract, Korean Patent No. 0511494, which discloses sewage extract, and the like.

The present invention also discloses an atopic dermatitis improving agent using a natural extract.

The technical problem of the present invention is to provide an atopic dermatitis improving composition.

The specific technical problem of the present invention will be presented below.

In order to solve the above technical problem, the present inventors first mix water and / or glucose with rice bran powder, as confirmed in the following examples, and aspergillus duck, Lactobacillus vulgaris and / or Streptococcus spp. Mophilus was inoculated to obtain the fermented product.

Then, each of the above fermented products (① fermentation product of Aspergillus duck material of rice bran powder, ② lactobacillus vulgaris fermentation product of rice bran powder, ③ fermentation product of Streptococcus rice bran powder, ④ aspergillus duck material of rice bran powder) Fermented product obtained by fermenting the fermented product again with Lactobacillus vulgaris, ⑤ Fermented product obtained by re-fermenting the fermented product of Aspergillus duck material of rice bran powder, and ⑥ Aspergillus duck of rice bran powder A fermentation product of ash was fermented by Lactobacillus Bulgaricus and Streptococcus thermophilus), to prepare a cosmetic lotion composition, and the composition was applied to patients suffering from atopic dermatitis. As a result, the inventors found that atopic dermatitis was more pronounced than rice bran powder in which all of the fermented products were not fermented. It was confirmed jinim for improvement.

The present invention is provided based on these experimental results.

Therefore, the atopic dermatitis improving composition of the present invention is characterized in that it comprises rice bran fermentation obtained by fermenting rice bran powder with Aspergillus duck, Lactobacillus vulgaris and / or Streptococcus thermophilus.

In the present specification, including the claims, the "branch rice" is a by-product obtained in the process of refining from brown rice to white rice, and refers to the inclusion of rice bran and whistle layer. It is to be understood that the rice bran and the whistle layer are included in the rice bran even if they additionally include the rinds and / or seedlings forming the rice bran (the outer shell of brown rice) and the endosperm and / or the endosperm.

In addition, in the present specification, including the claims, the term "atopic dermatitis" is defined to include all types of diseases classified as atopic dermatitis in the art, regardless of the direct or indirect cause of its occurrence. Atopic dermatitis is generally classified into infantile atopic dermatitis, pediatric atopic dermatitis, adult atopic dermatitis, and maternal atopic dermatitis, depending on the time of its onset or subject of the invention, where atopic dermatitis is all these types. It should be understood as including atopic dermatitis. This is because the atopic dermatitis improving agent composition of the present invention showed an itch improvement effect for up to 87% of randomly selected patients, as the following experimental example shows.

In addition, in this specification, including the claims, the "improvement" is meant to include treatment, prevention or alleviation of symptoms.

Also in the context of the present specification, including the claims, the meaning of the term "Brain Fermentation" means that it is fermented with the microorganisms listed above: Aspergillus duck, Lactobacillus vulgaris and / or Streptococcus thermophilus. As long as it is obtained, it is understood that no additives are added to the rice bran powder to promote fermentation during the fermentation process. It was fermented by adding glucose to promote fermentation by Aspergillus duckwood, as shown in the following Examples and Experimental Examples, even when glucose was not added (i.e., aspergillus duckwood in a mixture of rice bran powder and water). Even when the fermentation was added), the atopic dermatitis improved effect was less than the fermented product with the addition of glucose, but was confirmed to be significantly higher than the unfermented rice bran powder.

It is also understood that in the manufacture of rice bran fermentation, any further steps are included to facilitate fermentation. In the following Examples and Experimental Examples, although the effect of atopic dermatitis improved slightly when the rice bran powder was fermented with malt and then fermented without the saccharification, the effect of improving the atopic dermatitis was not fermented. It was still higher than the powder.

It should also be understood that in the production of rice bran fermentation, fermentation time and fermentation temperature are irrelevant. In the following examples, the rice bran fermented product was obtained by fermentation at a specific temperature and for a specific time, but all the rice bran fermented products obtained in the following examples had a higher atopic improvement effect than the rice bran powder which had not been fermented. As long as fermentation occurs, it is obvious that the resulting rice bran fermentation product will have a higher atopic improvement effect than the rice bran fermentation product.

However, preferably, the rice bran fermentation product is obtained by fermenting rice bran powder with Aspergillus duck, followed by fermentation with Lactobacillus vulgaris and / or Streptococcus thermophilus, more preferably aspergillus bran powder. It refers to what is obtained by fermentation with Lactobacillus vulgaris and Streptococcus thermophilus after fermentation with a duck material, most preferably after fermentation of rice bran powder with Aspergillus duck material under the specific fermentation process described in the following Examples. Means obtained by fermentation with Lactobacillus bulgaricus and Streptococcus thermophilus.

On the other hand, the composition for improving atopic dermatitis of the present invention may include the rice bran fermentation, the active ingredient in any amount, depending on the purpose, formulation, formulation purpose, etc., usually 0.01 weight based on the total weight of the composition % To 99.99 weight%.

In a specific embodiment, the composition for improving atopic dermatitis of the present invention can be understood as a cosmetic composition.

When the composition for improving atopic dermatitis of the present invention is understood as a cosmetic composition, the cosmetic composition may be prepared in various forms, for example, emulsion, lotion, cream (oil-in-water type, water-in-oil type, multiphase), solution , Suspensions (anhydrous and aqueous), anhydrous products (oil and glycols), gels, masks, packs or powders and the like.

The cosmetic composition for improving atopic dermatitis of the present invention may include an acceptable carrier in cosmetic preparations in addition to rice bran fermentation products.

Herein, "acceptable carriers in cosmetic preparations" are compounds or compositions already known and used that may be included in cosmetic preparations, or compounds or compositions which will be developed in the future, which have no toxicity, instability or irritation more than the body can adapt to in contact with the skin. Say that.

The carrier may be included in the cosmetic composition for improving atopic dermatitis of the present invention from about 0.01% by weight to about 99.99% by weight based on the total weight thereof.

However, since the ratio varies depending on the form of the above-described preparation of the cosmetic of the present invention and its specific application site (face, hand, etc.), its preferred application amount, etc., the ratio is in any aspect to the present invention. It should not be understood as limiting the scope of.

Meanwhile, examples of the carrier include alcohols, oils, surfactants, fatty acids, silicone oils, wetting agents, moisturizers, viscosity modifiers, emulsions, stabilizers, sunscreen agents, colorants, fragrances, and the like.

Alcohols, oils, surfactants, fatty acids, silicone oils, wetting agents, humectants, viscosity modifiers, emulsions, stabilizers, sunscreens, colorants, compounds / compositions that can be used as fragrances, etc., which can be used as the carrier are already known in the art. As a result, those skilled in the art can select and use appropriate materials / compositions.

In another specific embodiment, the composition for improving atopic dermatitis of the present invention can be understood as a pharmaceutical composition.

The pharmaceutical composition of the present invention may include pharmaceutically acceptable conventional ingredients in addition to the rice bran fermentation, which is an active ingredient, and these pharmaceutically acceptable conventional ingredients are commonly used in pharmaceutical preparations, and include lactose. , Dextrose, sucrose, sorbitol, mannitol, starch, acacia rubber, calcium phosphate, alginate, gelatin, calcium silicate, microcrystalline cellulose, polyvinylpyrrolidone, cellulose, water, syrup, methyl cellulose, methylhydroxy Benzoate, propylhydroxybenzoate, talc, magnesium stearate, mineral oil, and the like.

The pharmaceutical compositions of the present invention may also further comprise lubricants, wetting agents, sweetening agents, flavoring agents, emulsifying agents, suspending agents, preservatives and the like as additives. Components suitable for each use such as lubricants, wetting agents, etc. are already known in the art, so those skilled in the art can select and use appropriate components.

The ingredients may be included in the pharmaceutical composition of the present invention in an amount of 0.01% to about 99.99% by weight based on its total weight.

On the other hand, the pharmaceutical composition of the present invention can be administered by various routes, for example, orally or rectally, or by injection into veins, muscles, subcutaneous, intrauterine dura, and the like. Preferably it is applied topically to the affected area.

The pharmaceutical compositions of the invention may be prepared in unit dose form or may be prepared by incorporation into a multidose container. In this case, the formulation may be in the form of a solution, suspension, or emulsion, or may be in the form of elexirs, extracts, powders, granules, tablets, ointments, and the like.

The pharmaceutical composition of the present invention usually has a daily dosage in the range of 0.001 to 150 mg / kg body weight within a range that is not toxic to the human body, and may be administered once or in several divided doses. However, since the dosage of the pharmaceutical composition of the present invention is determined in view of various related factors such as the route of administration, the age, sex, weight of the patient, and the severity of the patient, the dosage may limit the scope of the present invention in any aspect. It should not be understood as.

In another specific embodiment, the composition for improving atopic dermatitis of the present invention can be understood as a soap composition.

When the composition for improving atopic dermatitis of the present invention is understood as a soap composition, the soap composition of the present invention may be prepared by adding a rice bran fermentation product to a soap base, and includes an skin moisturizer, an emulsifier, a water softener, and the like as an additive. Can be prepared.

As the soap base material, vegetable oils such as palm oil, palm oil, soybean oil, perm oil, olive oil, palm kernels, or animal oils such as tallow, pork, sunny and fish oils can be used, and the skin moisturizing agent is glycerin, erythritol and polyethylene glycol. , Propylene glycol, butylene glycol, pentylene glycol, hexyl glycol, isopropyl myristate, silicone derivatives, aloe vera, sorbitol and the like can be used, the emulsifiers include natural oils, wax fatty alcohols, hydrocarbons, natural plant extracts, etc. May be used, and tetrasodium idieti or the like may be used as the water softener.

The soap composition of the present invention may further include an antimicrobial agent, a dispersant, a foam inhibitor, a solvent, a scale inhibitor, a corrosion inhibitor, a perfume, a pigment, a metal ion blocking agent, an antioxidant, a preservative, and the like as an additive.

In the soap composition of the present invention, the soap base or additives may be included in an amount generally used in the art, and the soap base is generally 99.9 to 50 wt% based on the total content of the soap composition. Can be added.

As described above, according to the present invention can provide an atopic dermatitis improving agent composition using rice bran fermentation.

It will be described below with reference to Examples and Experimental Examples. However, the scope of the present invention is not limited to these examples and experimental examples.

< Example > Rice bran  Preparation of Fermentation Composition

Example 1 Preparation Example 1 of Rice Bran Fermentation Composition

Water is added to the rice bran powder obtained by crushing rice bran, which is a by-product of milling brown rice with white rice, in a weight ratio of 3: 1 (rice bran powder: water), and sterilized at 120 ° C. and 1.4 atm for 30 minutes. A rice bran fermentation composition was prepared by adding 50 ml of Aspergillus duckwood (1.0 × 10 ⁸ cell / ml) culture solution to about 1 kg of the sterilized rice bran powder and water mixture and fermenting at about 30 ° C. for 4 days. This fermented product was used in the following experimental example after sterilizing for 30 minutes at 120 degreeC and 1.4 atmospheres.

Example 2 Preparation Example 2 of Rice Bran Fermentation Composition

Water and glucose are added in a weight ratio of 3: 1: 0.3 (rice bran powder: water: glucose) in the rice bran powder obtained by crushing rice bran, which is a by-product of milling brown rice with white rice. After sterilization for 5 minutes, 50 ml of Aspergillus duckwood (1.0 X 10 ⁸ cell / ml) culture solution was added to about 1 kg of the sterilized rice bran powder, water and glucose mixture and fermented at about 30 ° C. for 4 days. Fermentation compositions were prepared. This fermented product was used in the following experimental example after sterilizing for 30 minutes at 120 degreeC and 1.4 atmospheres.

Example 3 Preparation Example 3 of Rice Bran Fermentation Composition

Water is added to the rice bran powder obtained by crushing rice bran, a by-product produced in the process of refining brown rice into white rice, in a weight ratio of 3: 1 (rice bran powder: water), and then roughly roughly equivalent to 1% of the weight of rice bran. Malt (malt) was added and glycosylated at about 60 ° C. for about 6 hours. The glycosylated saccharified solution was sterilized at 120 ° C and 1.4 atm for 30 minutes, and 50 ml of Aspergillus duckwood (1.0 X 10 ⁸ cell / ml) culture solution was added to about 1 L of the sterilized saccharified solution, and about 30 ° C. Fermentation for 4 days in to prepare a rice bran fermentation composition. This fermented product was used in the following experimental example after sterilizing for 30 minutes at 120 degreeC and 1.4 atmospheres.

Example 4 Preparation Example 4 of Rice Bran Fermentation Composition

To about 1 kg of the sterilized rice bran fermentation of <Example 2> 50 ml of Lactobacillus vulgaris (1.0 X 10 ⁸ Cell / ㎖) culture solution was added to ferment for 3 days at about 40 ℃ to prepare a rice bran fermentation composition. . This fermented product was used in the following experimental example after sterilizing for 30 minutes at 120 degreeC and 1.4 atmospheres.

<Example 5> Preparation of rice bran fermentation Composition Example 5

50 ml of Streptococcus thermophilus (1.0 X 10 ⁸ Cell / ml) culture solution was added to about 1 kg of the sterilized rice bran fermentation product of <Example 2>, followed by fermentation at about 40 ° C. for 3 days. Prepared. This fermented product was used in the following experimental example after sterilizing for 30 minutes at 120 degreeC and 1.4 atmospheres.

Example 6 Preparation Example 6 of Rice Bran Fermentation Composition

In about 1 kg of the sterilized rice bran fermentation of <Example 2> 1: 1 of Lactobacillus vulgaris (1.0 X 10 ⁸ Cell / ㎖) culture medium and Streptococcus thermophilus (1.0 X 10 ⁸ Cell / ㎖) culture solution 1: 1 ml of mixture was added and fermented at about 40 ° C. for 3 days to prepare a rice bran fermentation composition. This fermented product was used in the following experimental example after sterilizing for 30 minutes at 120 degreeC and 1.4 atmospheres.

< Production Example > Preparation of Cosmetic Water Composition

The ingredients in Table 1 were uniformly mixed to prepare a lotion composition including the rice bran fermentation composition of each example and the rice bran powder (not fermented) used in the examples.

TABLE 1

Preparation of Cosmetic Water Composition

division Preparation Example 1 Preparation Example 2 Preparation Example 3 Preparation Example 4 Preparation Example 5 Preparation Example 6 Comparative example Stearic acid 0.8 0.8 0.8 0.8 0.8 0.8 0.8 glycerin 5.0 5.0 5.0 5.0 5.0 5.0 5.0 Cetostearyl alcohol 2.0 2.0 2.0 2.0 2.0 2.0 2.0 Beta Glucan 6.0 6.0 6.0 6.0 6.0 6.0 6.0 Cetyl octanoate 4.0 4.0 4.0 4.0 4.0 4.0 4.0 Capryl / Capric Tree Cleanser 6.0 6.0 6.0 6.0 6.0 6.0 6.0 Squalane 4.0 4.0 4.0 4.0 4.0 4.0 4.0 Sorbitan stearate 0.4 0.4 0.4 0.4 0.4 0.4 0.4 Triethanol amine 0.1 0.1 0.1 0.1 0.1 0.1 0.1 Methylparaben 0.2 0.2 0.2 0.2 0.2 0.2 0.2 Incense incense 1.0 1.0 1.0 1.0 1.0 1.0 1.0 Fermented product of <Example 1> 2.0 Fermented product of <Example 2> 2.0 Fermented product of <Example 3> 2.0 Fermented product of <Example 4> 2.0 Fermented product of <Example 5> 2.0 Fermented product of <Example 6> 2.0 Unfermented Rice Bran Powder 2.0 Purified water to 100 to 100 to 100 to 100 to 100 to 100 to 100 * Unit is weight percent

< Experimental Example > Therapeutic Effect Test of Atopic Dermatitis

Twenty patients with atopic dermatitis were used as a group of experimental groups, and each of the experimental groups was applied to the cosmetic composition of each preparation for a total of 8 weeks each morning.

The results of a questionnaire on whether the atopic severity index (EISI score) and itching were alleviated after 8 weeks after the application of the cosmetic lotion composition of the preparation example were shown in Tables 2 and 3, respectively (Table 3). Figures in> indicate the proportion of subjects who have had itching).

The atopic severity index is determined by a dermatologist in the four stages of erythema, edema, stiffness, papules, abrasions, and sensitization on the body parts of the head, neck, upper limbs, torso, and lower extremities of atopic dermatitis patients. It is a numerical value that indicates the degree of skin lesions by grading and summing the area scores. The higher the value, the more severe the atopic symptoms. The lower the value, the better the skin improvement effect.

TABLE 2

EASI score

division Before application (average value) After application (average value) Preparation Example 1 4.7 3.9 Preparation Example 2 4.8 3.7 Preparation Example 3 5.3 3.9 Preparation Example 4 6.7 4.7 Preparation Example 5 6.5 4.9 Preparation Example 6 6.9 3.1 Comparative example 6.7 6.5

TABLE 3

Degree of improvement of itching

division Percentage of Subjects Who Improved Preparation Example 1 15% Preparation Example 2 18% Preparation Example 3 24% Preparation Example 4 35% Preparation Example 5 32% Preparation Example 6 87% Comparative example 7%

Looking at the <Table 2> and <Table 3> it can be seen that the effect of improving atopic dermatitis was very slight in the case of using non-fermented rice bran powder (in the case of Comparative Example). However, when the rice bran fermentation product is used, the effect of improving the atopic dermatitis is higher than that of the rice bran, which has not been fermented. In the case of Example 6), the improvement effect was the highest.

The present inventors assume that the fermentation of three types of rice bran powder has a high effect of improving atopic dermatitis due to the generation of new bioactive substances having good activity in improving atopic dermatitis during fermentation of rice bran powder. have.

Claims (6)

The mixture of water and glucose added to rice bran powder was sterilized, and the sterilized mixture was inoculated with Aspergillus duckwood and fermented. Atopic dermatitis improvement composition comprising the obtained rice bran fermentation product as an active ingredient. delete delete The method of claim 1, The composition is a cosmetic composition for improving atopic dermatitis. delete The method of claim 1, The composition is a soap composition for improving atopic dermatitis.