KR100908110B1 - Athlete's foot composition containing Nahanbaek essential oil - Google Patents

Abstract

The present invention is an essential oil containing anti-fungal fungal activity (Thujopsis dolobrata oil), that is, Nahanbaek that can prevent athlete's foot by inhibiting the growth of trichophyton mentagrophytes, the main causative agent of athlete's foot It relates to a composition for treating or preventing athlete's foot comprising an oil.

The composition contains Nahanbaek oil, which is used as an antimicrobial agent to prevent and treat athlete's foot by inhibiting the growth of Trichophyton mentagrophytes, and contains Thujopsis dolobrata oil in an amount of 8,000 to 200,000 ppm. It is characterized by comprising a heat-resistant oil of 0.01% to 20% by weight in a composition such as soap or aerosol spray.

Description

A composition using medical cure or precaution for athlete's foot containing Thujopsis dolobrata oil having antifungal}

The present invention relates to a composition for treating or preventing athlete's foot, including Nahanbaek essential oil, and more particularly, essential oil of Nahubaek (Thujopsis dolobrata sp.) Plant that inhibits the growth of Trichophyton mentagrophytes. It relates to a composition for treating or preventing athlete's foot.

Athlete's foot is one of the most common symptoms of fungal infections. The causative agents of athlete's foot eczema are trichophyton rubrum, trichophyton metagrophyte and epidermophytone phlocosome, among the fungi (dermatophytes) having affinity for skin, and they penetrate keratin of skin and cause itching, redness, inflammation, etc. It is said to cause symptoms.

The exacerbation of these dermatophite infections depends on the environment of the site of infection and the individual's ability to immunize.

Closures by shoes, socks, etc. increase the heat and humidity of the affected area and promote the growth of dermatophyte.

Athlete's foot is a parasitic and multiplying skin disease that fungus dissolves and exfoliates the skin's keratin. The fungus's keratin is rich and moist and warm (toes, soles, nails, flanks, around the groin, and flesh overlap). It occurs in most parts of the body. In general, it is easy to think that only the foot athlete's foot is athlete's foot, but it includes not only the feet and hands, but also the stiffness around the groin, the tinnitus on the nails of the nails, the tinea on the head, and the tincture on the body.

Athlete's foot is a very common skin disease, and statistically, more than 10% of the population suffers from athlete's foot, most frequently in their 20s and 40s. Rarely occurs in children.

Athlete's foot is not a negligible disease, and if left untreated, the fungus can penetrate other parts of the body, such as the instep or toenails. If not properly rooted, they tend to continue to recur and often become 'compatibility diseases' that have to live together for the rest of their lives.

Athlete's condition suddenly worsens when the temperature is about 15 degrees and the humidity is around 70%. Usually it starts to feel more itchy at the beginning of the rainy season. Athlete's most probable environment is 37 degrees Celsius, moderate humidity, and nutrients. Summer has all of these conditions, so the athlete's fungus multiplies in the summer. However, the fungus sticks to the skin even in winter, but only weakens its activity. This is because fungi, which had a strong propagation ability in a humid and warm environment, cannot hide well in winter and hide in the skin.

According to Clin. Exp. Dermatol. 17, 1992, such athlete's foot eczema is more pronounced in adults than in young children, and 10 to 15% of the adult population of developed countries have athlete's foot eczema or have been infected.

 In order to treat athlete's foot eczema, a method of killing the causative agent of athlete's foot eczema or inhibiting the growth of bacteria is considered. In this case, a keratin softener may be used together to remove stratum corneum where the athlete's foot fungus lives or to improve symptoms. Also, by washing and drying the affected area well, lowering the heat and humidity of the infected area to create an unfavorable condition for the growth of athlete's foot eczema may also help treat athlete's foot eczema.

As a treatment for athlete's foot eczema, antifungal agents and salicylic acid-prescribed external preparations, ointments, and sprays have already been developed and commercialized. However, athlete's foot is difficult to cure without recurring treatment without recurring symptoms.

Therefore, in order to completely treat athlete's foot eczema, it is necessary to continue treatment with antifungal agents even after symptom improvement to prevent re-infection.

The present inventors completed the present invention by confirming that Nahanbaek oil has antifungal activity against athlete's foot.

The present invention has been made to solve the above problems, the object of the composition is to kill allergens that cause skin allergy due to the overexposure of chemicals due to industrialization and to promote skin regeneration action to treat allergies and athlete's foot fungus To provide a composition for the treatment or prevention of athlete's foot using essential oil components of the plant of the genus Thujopsis.

In addition, Nahanbaek essential oil, which is very useful for the prevention or treatment of athlete's foot, has an antimicrobial effect on Trichophyton mentagrophytes, which is a causative agent of athlete's foot, and contains Nahanbaek essential oil when preparing a conventional soap or aerosol spray composition. It is to provide a composition for treating or preventing athlete's foot containing oil.

According to a feature of the present invention for achieving the above object, trichophyton mentagrophytes (Trichophyton mentagrophytes) by inhibiting the growth of athlete's foot fungi containing thujopsis dolobrata oil in an amount of 200,000 ppm that can prevent or treat athlete's foot antibacterial activity Eggplant provides a composition for treating or preventing athlete's foot containing Nahanbaek oil used as an antimicrobial agent.

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The composition for the treatment and prevention of athlete's foot containing Nahanbaek oil according to the present invention is a composition of soap or aerosol spray using Nahanbaek oil having antibacterial activity against Trichophyton mentagrophytes, the causative agent of athlete's foot. It is effective in treating or preventing athlete's foot.

The present invention is an essential oil containing Nahanbaek oil having an active athlete's foot fungus, that is, athlete's foot including Nahanbaek oil that can prevent athlete's foot by inhibiting the growth of Trichophyton mentagrophytes, which is the main causative agent of athlete's foot It relates to a composition for treatment or prophylaxis.

The present inventors confirmed that including Nahanbaek oil in the prophylactic and therapeutic products of athlete's foot such as soap or aerosol spray can effectively prevent or treat athlete's foot by inhibiting the antibacterial activity of athlete's foot.

Examples of compositions according to the invention are compositions for the prevention and treatment of athlete's foot, such as soaps, aerosol sprays, the composition of which can be determined without difficulty by one of ordinary skill in the art.

1 is a culture photograph showing the antibacterial activity of Nahanbaek oil against Athlete's foot (T.mentagrophytes) by the composition of the present invention, Figure 2 is a picture of Nahanbaek oil against athlete's foot (T.mentagrophytes) by the composition of the present invention Figure 3 is a graph showing the concentration-dependent antimicrobial activity, Figure 3 shows a culture photograph of the athlete's foot antibacterial activity of griseofulvin (GFV) as a comparative group, Figure 4 shows the concentration dependency of griseofulvin (GFV) to T.mentagrophytes as a comparison group The graph of antimicrobial activity is shown.

Hereinafter, exemplary embodiments of the present invention will be described in detail with reference to the accompanying drawings.

In the antibacterial activity test for athlete's foot fungus of Nahanbaek oil of the present invention, trichophyton mentagrophytes (KCTC 6316), the causative agent of athlete's foot fungus, were distributed, and lyophilized powder cells were dissolved in distilled water to each solid medium. Three-point smearing is incubated to the culture conditions (28 ° C).

The athlete's foot was used as a medium published by the place of purchase (BRC-KCTC BRC-KCTC), all the medium was sterilized at 121 ℃, 15 minutes, injected into a petri dish was performed as a solid plate medium, liquid As a medium, broth of the same composition without agar powder was used. Athlete's foot cultured by three-point smearing is partially stored and liquid fungus is cultured again for athlete's foot growth inhibition experiment.

The cells were lyophilized and streaking in a solid medium using a holding loop, followed by incubation at a specified temperature and incubation days. After incubation, a single colony was inoculated into the liquid medium.

After inoculation into the liquid medium, the culture was carried out in a shaker at 30 ° C., and the growth of the strain was measured at 600 nm using a UV / VIS spectrophotometer. Antimicrobial activity was measured.

TABLE 1

Athlete's foot composition table

  Badge number 340  Badge Name Sabourauds (Agar)  Furtherance Peptone 10.0 g Glucose 40.0 g Distilled water 1 liter (Agar 20.0 g)

Experimental Example 1.

Antifungal Activity Measurement

A single colony of the strains cultured above is taken and inoculated in 2 ml sterile liquid medium. After incubation by incubation in a shaking incubator or incubator for a specified temperature and incubation time, the culture medium is diluted to adjust the bacteria to absorbance at 600 nm.

Take 200 ~ 300 µl of the liquid medium and spread it evenly on a solid medium using a spreader. Place a sterile diameter 6 mm filter paper disc on the plate. 5 μl of each essential oil sample is injected into the filter paper disc, inhaled, placed on a clean bench, and incubated in the incubator for 48 hours at 28 ℃ (or 28 ～ 30 ℃) according to the incubation time of each strain. After incubation, antifungal activity was confirmed by measuring the diameter (mm) of the clear zone (CZ) around the filter paper disc.

As a result, it can be seen that Nahanbaek oil has an antimicrobial effect in a test strain of Tricophyton mentagrophytes.

In particular, as shown in FIG. 2, when Thujopsis dolobrata oil is 200,000 ppm, the clear zone is the largest, and when 8,000 ppm, the clear zone is small. That is, the higher the concentration, the larger the clear zone can be seen.

TABLE 2

Strain # Name Concentration (ppm) remark  6316 T.mentagrophytes One hundred oil 2 200,000 (1) 40,000 (1´) 8,000 (1˝) Clear zone 31.5 ± 2.12 20 ± 0 11.5 ± 0.7

 -Clear zone measurement results for antibacterial activity of Nahanbaek oil against T.mentagrophytes (KCTC 6316)

In addition, as shown in Figure 4 attached Thujopsis dolobrata oil as a control for trichophyton mentagrophytes (Trichophyton mentagrophytes, KCTC 6316) as a control for the results of the antimicrobial activity of each concentration of griseofulvin (GFV) Is shown in Table 3.

TABLE 3

Strain # Name Concentration (ppm) remark 6316 1 (10,000) 2 (1,000) 3 (100) griseofulvin (GFV) 21.3 ± 5.13 17.3 ± 6.81 11 ± 4.58 Clear zone (mm) Figure 4

 -Result of antimicrobial activity of griseofulvin (GFV) substance

As shown in FIG. 3, the griseofulvin (GFV) also has antibacterial activity in a test strain of Tricophyton mentagrophytes (KCTC 6316).

In particular, as shown in Table 3, when griseofulvin (GFV) is 10,000 ppm, the clear zone is the largest, and when it is 100 ppm, the clear zone is small. That is, the higher the concentration, the larger the clear zone can be seen.

Nahanbaek oil and the control group griseofulvin (GFV) according to the present invention has an inhibitory effect of tricophyton mentagrophytes (Trichophyton mentagrophytes), the higher the concentration can be seen that the growth zone (clear zone) appears large.

In other words, it can be seen that Nahanbaek oil has an antimicrobial effect against T. mentagrophytes (T. mentagrophytes) can be composed of a composition that can prevent or treat athlete's foot.

Therefore, the composition for treating and preventing athlete's foot against the antibacterial activity of Trichophyton mentagrophytes bacteria with respect to the Nahanbaek oil of the present invention, that is, prepared by containing Nahanbaek Oil in the composition of soap or aerosol spray can do.

Formulation Example 1. Soap

In order to prepare soap for treating and preventing athlete's foot containing Nahanbaek oil of the present invention, it may be prepared according to a manufacturing method in a conventional soap manufacturing field as described in Table 4 below.

TABLE 4

Ingredient Content (% by weight) Nahanbaek oil 0.1 to 20% Ketoconazole 4 % Purified water 2 % Tricholic acid 2 % Mineral oil One % Polyethylene glycol 0.5% Spices 0.1% Tribromsala 0.1% Hexachlorophene 0.1% Ethylenediamine Acetate 0.1% Titanium dioxide 0.1% Soap base up to 100% Sum 100

Formulation Example 2. Aerosol Spray

In order to prepare aerosol sprays for treating and preventing athlete's foot containing the hanbaek oil of the present invention, it can be prepared according to the manufacturing method in the conventional aerosol spray manufacturing field as described in Table 5 below.

TABLE 5

Ingredient Content (% by weight) Nahanbaek oil 0.1 to 20% Purified water 20.0% Surfactants 0.1% or more Alkali metal benzoate 0.01% or more Hydrocarbon Gas Propellant up to 100% Sum 100

The present invention is not limited to the embodiments described above, and various modifications and changes can be made by those skilled in the art, which are included in the spirit and scope of the present invention as defined in the appended claims.

1 is a photograph showing the antimicrobial activity of Nahanbaek oil against athlete's foot (T.mentagrophytes) by the composition of the present invention

Figure 2 is a graph showing the concentration-dependent antibacterial activity of Nahanbaek oil against athlete's foot (T.mentagrophytes) by the composition of the present invention

Figure 3 is a culture photograph showing the fungal antibacterial activity of griseofulvin (GFV) as a comparative group

Figure 4 is a graph showing the concentration-dependent antibacterial activity of griseofulvin (GFV) against athlete's foot (T.mentagrophytes) as a comparative group

Claims (3)

Trichophyton mentagrophytes (Trichophyton mentagrophytes) At least 200,000 ppm of Thujopsis dolobrata oil, which inhibits the growth of athlete's foot, can be prevented or cured of athlete's foot. Therapeutic or prophylactic composition delete delete

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