KR100887590B1 - Sample attaching device - Google Patents

Abstract

Disclosed is a biological sample attachment device. The biological sample attachment device of the present invention comprises: a filter accommodating portion in which a filter is accommodated; A storage container operating unit including a storage container holder capable of mounting the storage container, and a storage container conveying device capable of horizontally and vertically transferring the storage container holder; A storage container lid opening portion formed at a lower side of the storage container holder and capable of opening a lid of the storage container mounted on the storage container holder; A filter operation unit formed at a lower side of the storage container lid opening and configured to communicate with the filter storage unit to mount a filter supplied from the filter storage unit, and to move horizontally and vertically; An air adjusting unit formed at a position adjacent to the filter operating unit and movable vertically for close contact with the filter mounted on the filter operating unit; Located on the upper side of the air control, characterized in that it comprises a slide operation unit having a slide holder for mounting the slide, and a slide transfer device for transferring the slide holder horizontally and vertically.

Sample, Attachment, Preservation Container, Filter, Slide

Description

Sample Attaching Device

1 is a perspective view schematically showing the structure of a biological sample attachment device according to an embodiment of the present invention.

2 is a view showing a filter housing according to an embodiment of the present invention.

3 is a view showing a storage container operating unit according to an embodiment of the present invention.

Figure 4 is a view showing another container opening storage lid in an embodiment of the present invention.

5 is a view showing a filter control unit and an air control unit according to an embodiment of the present invention.

6 is a view illustrating a slide operation unit and a slide storage unit according to an embodiment of the present invention.

7 is a view showing a centrifugal separator according to an embodiment of the present invention.

8 is a view illustrating a process of fusion of the biological sample in the storage container according to the invention to the filter.

9 is a view illustrating a process of attaching a biological sample fused to a filter to a slide according to the present invention.

10 is a view showing a combined state of the storage container according to an embodiment of the present invention.

11 is a view showing an exploded state of the storage container according to an embodiment of the present invention.

12 is a diagram illustrating a filter according to an embodiment of the present invention.

13 is a view showing a conventional biological sample attachment process.

Explanation of symbols on the main parts of the drawings

 10: biological sample attachment device 100: filter housing

200: storage container operation unit 300: storage container lid opening

400: filter operation unit 500: air control unit

600: slide operation unit 700: slide storage unit

800: centrifuge 810: display unit

820: control panel 900: storage container

950 filter

The present invention relates to a biological sample attachment device, and more particularly, to a biological sample attachment device in which a process of attaching the collected biological sample to a slide with a thin and uniform thickness without contamination is performed by an automatic means.

FIG. 13 is a view illustrating a conventional biological sample attachment process. Referring to this, the storage container 1 is a container containing the collected biological sample. In order to mix the biological sample, the user must shake the manual storage container 1 by hand. . Since the storage container 1 is limited in its use to store the liquid, when the biological sample is transferred to the slide, a separate container 2 for accommodating the liquid is required, and the container 2 is open at the upper side. Because of this structure, contact with air is inevitable.

In addition, the filter 4 with the collecting membrane 3 attached to the upper side is provided at the lower side of the container 2, and the piston 6 is supported by the main body 5 and supports the filter 4 and enters into the main body 5. ), The liquid in the container 2 is sucked in.

As described above, a storage container 1 for dispersing a biological sample attached to a collecting brush in a liquid is conventionally required, and the container is shaken by shaking the storage container 1 to separate the liquid in a state where the biological sample is sufficiently spread in the liquid. Move on. The filter 4 is provided below the container 2, and the biological sample is caught by the sampling membrane 3 of the filter 4 by suction of the liquid by the piston 6 below the filter 4.

In order to attach the biological sample filtered on the collecting membrane 3 to the slide, the container 2 and the main body 4 must be separated. At this time, the liquid left in the container 2 flows down and cannot be reused. In addition, the entire process of attaching the biological sample in the storage container (1) to the slide is manually performed by the user, so it takes a long time, and impurities may be transferred during each process, thereby reducing the accuracy of the sample inspection. Therefore, there is a need for improvement.

The present invention has been created by the necessity as described above, and relates to a biological sample attachment device that the process of attaching the collected biological sample to the slide with a thin and uniform thickness without contamination in order to increase the accuracy of the sample inspection is made by automation means. .

Biological sample attachment device according to the present invention:

A filter accommodating part accommodating a filter; A storage container operating unit including a storage container holder capable of mounting a storage container, and a storage container conveying device capable of horizontally vertically transferring the storage container holder; A storage container lid opening portion formed under the storage container holder and capable of opening a lid of the storage container mounted on the storage container holder; A filter operation unit formed at a lower side of the storage container lid opening and configured to communicate with the filter accommodating part to mount the filter supplied from the filter accommodating part, and to move horizontally and vertically; An air adjusting unit formed at a position adjacent to the filter operation unit and movable vertically for close contact with the filter mounted on the filter operation unit; Located on the upper side of the air control unit, and includes a slide holder for mounting a slide and a slide operation unit having a slide feeder for transferring the slide holder horizontally and vertically.

Preferably, the slide storage unit further comprises a slide storage unit for separating and storing the slide attached to the biological sample from the slide holder.

Preferably, the method further includes a centrifugal separator for centrifuging the biological sample stored in the storage container evenly in the storage liquid before placing the storage container on the storage container holder.

Preferably, the storage vessel holder and the upper cradle is mounted one end of the storage vessel; It is provided with a lower cradle to which the other end of the storage container is mounted, and in the upper cradle and the lower cradle, a portion of the contact with the outer surface of the storage container is formed with a spring ball for firmly fixing the storage container.

Preferably, the storage container transfer device to transfer the storage container holder to the storage container lid opening side when the lid of the storage container to open, the storage container when the storage container is to be in close contact with the filter. The cradle is transferred to the filter operation unit on which the filter is mounted.

Preferably, the storage container lid opening is opposed to the storage container holder, the insertion projection corresponding to the insertion groove portion of the storage container lid is formed, the rotatable swivel; It is formed on the lower side of the swivel and provided with a support for supporting the swivel, the opening of the storage container lid after the close coupling of the insertion groove and the insertion projection by the transfer to the storage container lid opening of the storage container holder It is made by the rotation of the swivel.

Preferably, the filter operation unit and the vertical transfer unit that can mount the filter, the vertical transfer of the filter; It is formed on the lower side of the vertical conveying portion, and has a horizontal conveying portion capable of conveying the filter horizontally.

Preferably, the air control unit further includes a vibration generating unit for generating a fine vibration during the fusion process so that the biological sample in the storage container is easily fused to the filter.

Preferably, when the biological sample in the storage container is to be fused to the filter, the storage container operation unit and the filter operation unit are respectively transported so that the storage container and the filter are in close contact with each other.

More preferably, when the storage container is in close contact with the filter, the air control unit is combined with the lower side of the filter to suck air from the filter side.

Preferably, the slide holder further includes a temperature holding unit for maintaining the slide at an appropriate temperature so that the biological sample is easily attached to the slide.

Preferably, when the biological sample fused to the filter is to be attached to the slide, the filter operation unit and the slide operation unit are respectively transferred to closely adhere the filter and the slide.

More preferably, when the filter and the slide are in close contact with the air control unit is combined with the filter lower side to supply air to the filter side.

Hereinafter, with reference to the accompanying drawings will be described an embodiment of a biological sample attachment device according to the present invention. In this process, the thickness of the lines or the size of the components shown in the drawings may be exaggerated for clarity and convenience of description. In addition, terms to be described later are terms defined in consideration of functions in the present invention, which may vary according to the intention or convention of a user or an operator. Therefore, the definitions of these terms should be made based on the contents throughout the specification.

Before describing the biological sample attachment device according to the present invention will be described with respect to the storage container and filter used in the device.

10 is a view showing a combined state of the storage container according to an embodiment of the present invention, Figure 11 is a view showing an exploded state of the storage container according to an embodiment of the present invention, Figure 12 is a view of the present invention 1 is a diagram illustrating a filter according to an embodiment.

10 and 11, the storage container 900 is a place where the biological sample collected from the target organism is stored and stored in a preservation liquid, and includes a lid 910, a filtration membrane mounting part 920, and a body 930.

The lid 910 forms a disc shape, and an insertion groove 911 into which the insertion protrusion 311 of the storage container lid opening 300 to be described later is inserted is formed at the center of one surface thereof. In addition, the insertion groove 911 is formed with a locking plate 912 which is caught with the insertion protrusion 311 when the insertion protrusion 330 is rotated by the rotation of the rotary table 310, the lid 910 of the storage container 900 is It can be opened. The other surface of the lid 910 is formed with a threaded portion 913 extending outward for coupling with the filter membrane mounting portion 920.

The filtration membrane mounting portion 920 is formed with a plurality of locking grooves 921 on the outer surface, the spring ball 214 of the storage container holder 210 to be described later is seated in the locking groove 921 to press the filter membrane mounting portion 920. As a result, the storage container 900 can be firmly fixed to the storage container holder 210. A threaded portion 922 is formed at an inner circumference of the outer surface of the filtration membrane mounting portion 920 to correspond to the threaded portion 913 of the lid 910 for coupling with the lid 910. A cylindrical protrusion 923 extending outward is formed near the center of the filtration membrane mounting part 920, and a filtration membrane made of a plurality of minute pores to prevent the liquid from passing rapidly. 924 is formed. Since the biological sample in the storage container 900 must pass through the filtration membrane 924 in order to be fused to the filter 950, the diameter of the pores of the filtration membrane 924 should be larger than the size of the target biological sample.

Body 930 is formed in a cylindrical shape, a plurality of stirring wings 931 are formed on the inner surface for more even stirring during centrifugation. The filtration membrane mounting portion 920 and the body 930 are coupled to each other, the threaded portion 932 is formed on the outer surface of the body 930, correspondingly the threaded portion (not shown) on the inner surface of the filtration membrane mounting portion 920 ) Is formed.

Referring to FIG. 12, the filter 950 is for permeating the preservative liquid in the biological sample and the preservative solution contained in the storage container 9900 and filtering only the biological sample. The horizontal plate 951, the extension 952, the protrusion 953, Membrane membrane 954.

The horizontal plate 951 is formed in a plate shape having a rectangular cross section, and guide grooves 955 are formed at each side thereof, and are guided by the guide rails 130 of the filter accommodating part 100 to be described later.

On one side of the horizontal plate 951 is formed a cylindrical extension portion 952 extending outward, and the other side is also formed with a protrusion 953 extending outwardly.

An upper side surface of the protrusion 953 is formed to be concave inward, and a plurality of small holes 956 are formed.

The membrane membrane 954 penetrates the reservoir solution contained in the reservoir 900 and is coupled to the upper edge of the protrusion 953 to filter only the biological sample. Each of the plurality of minute pores constituting the membrane membrane 954 is a biological It should have a smaller diameter than the sample.

1 is a perspective view schematically showing the structure of a biological sample attachment device according to an embodiment of the present invention.

Referring to FIG. 1, the biological sample attachment device 10 of the present invention includes a filter accommodating part 100, a storage container operation part 200, a storage container lid opening part 300, a filter operation part 400, , An air conditioner 500, a slide operation part 600, a slide storage part 700, and a centrifuge part 800.

2 is a view showing a filter housing according to an embodiment of the present invention.

1 and 2, the filter accommodating part 100 is formed to be able to enter and exit into the main body of the biological sample attachment device 10, and a pair of filter laminates 110 formed to face each other from the upper side to the lower side. ) Is formed. In the present embodiment, two pairs of filter laminates 110 are provided, but the present invention is not limited thereto. One or three filter laminates 110 may be provided.

A lowering prevention jaw (not shown) is formed at the bottom of the filter laminate 110 to prevent the filter 950 from falling further, and the guide groove 955 of the filter 950 is formed at the inner side of the filter laminate 110. A guide rail 130 corresponding to the guide rail 130 is formed to guide the falling down of the filter 950.

3 is a view showing a storage container operating unit according to an embodiment of the present invention.

1 and 3, the storage container operating unit 200 includes a storage container holder 210 and a storage container conveying device 220.

The storage container holder 210 is a place where the storage container 900 is mounted in a state in which the lid 910 faces downward, and includes an upper holder 211 and a lower holder 212 having a U shape. Spring balls 213 and 214 are formed at portions of the upper cradle 211 and the lower cradle 212 in contact with the storage container 900 such that the storage container 900 is firmly fixed to the storage container holder 210. Specifically, the spring ball 213 of the upper cradle 211 is to press the body 930 of the storage container 900, the spring ball 214 of the lower cradle 212 is caught by the side of the filter membrane mounting portion 920 The storage container 900 is firmly fixed to the storage container holder 210 by being seated in the groove 921 to pressurize it.

The storage container transfer device 220 is formed on one side of the storage container holder 210, and is a device for horizontally transferring the storage container holder 210 vertically.

Figure 4 is a view showing another container opening storage lid in an embodiment of the present invention.

1 and 4, the storage container lid opening 300 is formed on the lower side of the storage container holder 210 to open the lid 910 of the storage container 900 mounted on the storage container holder 210. It may be, and consists of a rotary table 310 and the pedestal (320).

Swivel table 310 is rotatably configured to open the lid 910, the center is formed concave to correspond to the appearance of the lid 910. In the concave surface of the swivel table 310, an insertion protrusion 311 inserted into the insertion groove 911 of the lid 910 is formed to protrude.

Pedestal 320 is a configuration formed on the lower side of the rotary table 310 to support the rotary table (310).

5 is a view showing a filter control unit and an air control unit according to an embodiment of the present invention.

1 and 5, the filter operation unit 400 is formed below the storage container lid opening 300 and is formed to communicate with the filter accommodation unit 100 to filter 950 from the filter accommodation unit 100. ) May be supplied, and the filter 950 may be horizontally and vertically transferred, and may include a vertical transfer part 410 and a horizontal transfer part 420.

The vertical transfer part 410 is a place where the filter 950 supplied from the filter accommodating part 100 is mounted, and when the biological sample in the storage container 900 is fused to the filter 950 or fused to the filter 950. When the biological sample is attached to the slide 970, the mounted filter 950 is formed to be movable vertically to be in close contact with the storage container 900 or the slide 970.

The horizontal transfer unit 420 is configured to be horizontally moved, and is formed below the vertical transfer unit 410. The vertical conveying part 410 and the horizontal conveying part 420 are connected by the vertical conveying axis 430, and the vertical conveying part 410 is vertically conveyed with respect to the horizontal conveying part 420 by the operation of the vertical conveying axis 430.

The air conditioner 500 is positioned adjacent to the filter operation unit 400, is formed in a cylindrical shape, and is configured to be vertically movable. The upper side of the air control unit 500 is formed with a communication unit 510 extending upward.

When the biological sample in the storage container 900 is to be fused to the filter 950, the storage container 900 and the filter 950 are brought into close contact by the transfer of the storage container holder 210 and the vertical transfer unit 410, respectively. The communicating portion 510 of the air cutout 500 is inserted into the extension 952 of the filter 950. Thereafter, when the air on the filter 950 side is sucked by the operation of the air conditioner 500, a pressure difference is generated between the storage container 900 and the filter 950, whereby a biological sample in the storage container 900 is generated. Is transmitted through the filtration membrane 924 and fused onto the membrane membrane 954 of the filter 950. On the other hand, the air control unit 500 is provided with a vibration generating unit 520 for generating a fine vibration, the air control unit coupled with the filter 950 to facilitate this when the biological sample is fused to the filter 950 ( To generate a small vibration.

6 is a view illustrating a slide operation unit and a slide storage unit according to an embodiment of the present invention.

1 and 6, the slide control unit 600 includes a slide holder 610, a slide feeder 620, and a temperature maintaining unit 630.

The slide holder 610 is a place where the slide is mounted, the slide transfer device 620 is a device that can transfer the slide holder 610 horizontally and vertically. The temperature maintaining part 630 is embedded in the slide holder 610, and maintains the mounted slide at an appropriate temperature so that the biological sample can be easily attached to the slide.

The slide storage unit 700 is located at the lower side of the slide operation unit 600, and is a space for separating and storing the slide on which the biological sample is attached from the slide holder 610.

7 is a view showing a centrifugal separator according to an embodiment of the present invention.

1 and 7, the centrifuge 800 is centrifuged to evenly spread the biological sample contained in the stock solution in the stock container 900 before placing the stock container 900 in the stock container holder 210. Wherein, in the present embodiment is disclosed as being formed on the right side of the biological sample attachment device 10, but is not limited thereto.

Hereinafter will be described the operating principle of the biological sample attachment device according to an embodiment of the present invention. FIG. 8 is a view illustrating a process of fusion bonding a biological sample in a storage container according to the present invention to a filter, and FIG. 9 is a view illustrating a process of attaching a biological sample fused to a filter to a slide according to the present invention.

The preservative liquid of the preservation vessel 900 is centrifuged through the centrifuge 800 so that the biological sample collected from the target organism is evenly spread in the preservation liquid contained in the preservation vessel 900.

The preservation vessel 900 containing the biological sample having the centrifugation process is completed is mounted on the preservation vessel holder 210 with the lid 910 facing downward. Insertion protrusion 311 of the rotating table 310 into the insertion groove 911 of the lid 910 by the storage container holder 210 is transferred to the storage container lid opening 300 by the operation of the storage container conveying device 220. ) Is inserted. Thereafter, the lid 910 is released from the filter membrane mounting portion 920 by the rotation of the rotary table 310, and the lid 910 is opened. On the other hand, the insertion groove 911 is formed with a locking plate 912 to prevent the insertion projections 311 when the rotation of the rotary table 310 is prevented from turning in the insertion groove 911.

After the opening of the lid 910 is completed, the storage container transfer device 220 and the filter operation unit 400 are operated to bring the filter membrane mounting unit 920 and the filter 950 of the storage container 900 into close contact with each other. The unit 500 is raised to insert the communication unit 510 into the extension 952 of the filter 950. Thereafter, the air on the filter 950 side is sucked into the air control unit 500 through the air control unit 500. As air on the filter 950 is sucked, a pressure difference occurs between the storage container 900 and the filter 950, and the biological sample in the storage container 900 passes through the filtration membrane 924 to allow the membrane of the filter 950 to pass through. Fused to the membrane 954. Membrane membrane 954 is composed of a plurality of fine pores, the diameter of each pore is smaller than the biological sample, so that even if the preservative liquid passes through the membrane membrane 954, the biological sample does not penetrate the membrane membrane 954 to be filtered Will be. In addition, in order to more easily and quickly fuse the biological sample from the storage container 900 to the membrane membrane 954, a process of generating minute vibrations by the vibration generator 520 installed in the air conditioner 500 is performed in parallel. do. On the other hand, if the biological sample is evenly fused to the membrane membrane 954, the control unit detects it and stops the air intake by the air control unit 500.

After completion of the fusion of the biological sample to the membrane membrane 954, the storage container holder 210 is returned to its original position by the storage container conveying device 220, the slide holder 610 by the slide feeder (620) filter operation unit ( The slide 970 and the filter 950 are brought into close contact with each other. At this time, since the communication part 510 of the air conditioner 500 is inserted and maintained in the extension part 952 of the filter 950, when the close contact is completed, the filter is operated by the operation of the air conditioner 500. Air is supplied to the 950 side. As air is supplied to the filter 950 side, the biological sample fused to the membrane membrane 954 is attached to the slide in a thin and uniform thickness.

After the attachment of the biological sample to the slide, the filter operation unit 400, the air control unit 500, and the slide operation unit 600 is returned to the original position, the slide mounted on the slide holder 610 is a slide feeder 620 It is separated from the slide holder 610 by the operation of the) is moved to the slide storage unit 700 located below the slide operation unit 400.

Although the present invention has been described with reference to the embodiments shown in the drawings, this is merely exemplary, and those skilled in the art to which the art belongs can make various modifications and other equivalent embodiments therefrom. Will understand. Therefore, the true technical protection scope of the present invention will be defined by the claims below.

As described above, the biological sample attachment device according to the present invention can prevent the attachment of contaminants, etc. that may occur during each process by automating the entire process of attaching the collected biological sample to the slide, unlike the conventional invention, The thickness of the biological sample attached to the slide can be made thin and uniform to increase the accuracy of the sample inspection. In addition, the present invention can provide a fine vibration upon fusion of the biological sample to the filter to facilitate faster and easier fusion of the biological sample. In addition, the present invention allows the biological sample to be more easily attached to the slide by maintaining the slide at an appropriate temperature.

Claims (13)

A filter accommodating part in which a filter is accommodated; A storage container operating unit including a storage container holder capable of mounting a storage container, and a storage container conveying device capable of horizontally vertically transferring the storage container holder; A storage container lid opening portion formed under the storage container holder and capable of opening a lid of the storage container mounted on the storage container holder; A filter operation unit formed at a lower side of the storage container lid opening and configured to communicate with the filter accommodating part to mount the filter supplied from the filter accommodating part, and to move horizontally and vertically; An air adjusting unit formed at a position adjacent to the filter operation unit and movable vertically for close contact with the filter mounted on the filter operation unit; Biological sample attachment device, characterized in that it comprises a slide operation unit which is located on the upper side of the air control unit, a slide holder for mounting the slide, and a slide transfer device for transferring the slide holder horizontally and vertically. The method of claim 1, Located on the lower side of the slide operation unit, the biological sample attachment device further comprises a slide storage unit for separating and storing the slide attached to the biological sample from the slide holder. The method of claim 1, And a centrifugal separator for centrifuging the biological sample stored in the storage container evenly in the storage liquid before placing the storage container in the storage container holder. The method of claim 1, The storage vessel holder and the upper cradle is mounted one end of the storage vessel; It is provided with a lower cradle to which the other end of the storage container is mounted, Biological sample attachment device, characterized in that the upper portion and the lower holder in contact with the outer surface of the storage container is formed a spring ball for firmly fixing the storage container. The method of claim 1, The storage container transfer apparatus transfers the storage container holder to the storage container lid opening side when the lid of the storage container is to be opened, and transfers the storage container holder to the filter when the storage container is in close contact with the filter. Apparatus for attaching a biological sample, characterized in that the transfer to the side of the filter operation. The method of claim 1, The storage container lid opening is opposed to the storage container holder, the insertion protrusion corresponding to the insertion groove of the storage container lid is formed, and a rotatable swivel; It is provided on the lower side of the swivel and has a support for supporting the swivel, The opening of the storage container lid is a biological sample attachment device, characterized in that made by the rotation of the swivel after the close coupling of the insertion groove and the insertion protrusion by the transfer to the storage container lid opening of the storage container holder. The method of claim 1, The filter operation unit may mount the filter, and a vertical transfer unit capable of vertically transferring the filter; And a horizontal transfer unit formed below the vertical transfer unit and capable of horizontally transferring the filter. The method of claim 1, The air conditioning unit further comprises a vibration generating unit for generating a fine vibration during the fusion process so that the biological sample in the storage container is easily fused to the filter. The method of claim 1, When the biological sample in the storage container is to be fused to the filter, the storage container operating unit and the filter operation unit by transferring each of the storage container and the filter, characterized in that the filter and the filter in close contact. The method of claim 9, And the air control unit is coupled to the lower side of the filter when the storage container is in close contact with the filter to suck air from the filter side. The method of claim 1, The slide holder further comprises a temperature holding unit for maintaining the slide at an appropriate temperature so that the biological sample is easily attached to the slide. The method of claim 1, When the biological sample fused to the filter is to be attached to the slide, the filter operation unit and the slide operation unit to transfer the filter and the slide, characterized in that the filter and the slide in close contact with each other. The method of claim 12, And the air control unit is coupled to the lower side of the filter when the filter is in close contact with the slide to supply air to the filter side.

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